A Herbal Formulation And A Method For The Prepaparation Thereof


Updated about 2 years ago

Abstract

The present invention deals with a herbal formulation comprising an extract of Cymbopogon winterianus (Java citronella), Lantana Camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus spp., Azadirachta indica.Ocimum kilimandscharicum and Veteveria zizanioides which is effective against mosquito, bacteria, virus and fungi. Effectiveness observed is 98% within 5hrs. Further, the present invention also deals with a method for the preparation of said formulation.

Information

Application ID 921/DEL/2006
Invention Field TRADITIONAL KNOWLEDGE BIOTECHNOLOGY
Date of Application 2006-03-31
Publication Number 14/2012

Applicants

Name Address Country Nationality
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH ANUSANDHAN BHAWAN, RAFI MARG, NEW DELHI-110001, INDIA. India India

Inventors

Name Address Country Nationality
SINGH RAJ SHEKHER CMRI, BARWA ROAD, DHANBAD-826001. India India
SINGH SHAILENDRA KUMAR CMRI, BARWA ROAD, DHANBAD-826001. India India
PAL DEBJIT CMRI, BARWA ROAD, DHANBAD-826001. India India
GUPTA ASHA CMRI, BARWA ROAD, DHANBAD-826001. India India

Specification

Field of the invention;
The present invention relates to the herbal formulation comprising extracts of the plants selected from the group comprising of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus sp., Azadirachta indica, Ocimum kilimandscharicum, Vetiveria zizanioides effective against mosquitoes, bacteria, viruses, fungi or mixed population thereof.
More particularly, the present invention relates to a process for the preparation of said herbal formulation.
Background and Prior art of the invention:
Herbal insecticides are in great demand due to increased pollution threat to soil and water bodies by insecticides laden with inorganic chemicals. Chemical insecticides cause a great damage to soil and water bodies by leaching of toxic chemicals. Sole reliance on and heavy usage of organochlorine, organophosphate, carbamate and pyrethroid insecticides has spawned environmental problems and triggered public concern. Ever-tightening regulations on pesticide use also point toward the need to develop alternative, less pesticide-centered management approaches. It is generally assumed, however, that pesticides provide a major non-point source of environmental pollutants. Current public concern about some chemicals in agriculture causing health and environmental problems, and the requirements of some overseas countries for no chemical residues in farm/agricultural products has reinforced the need to develop techniques to eliminate or reduce the present level of chemical inputs. The present invention is completely herbal, organic, degradable, non-polluting, easy to use, is cheap and affordable. Reference may be made to U.S. Pat. No. 6,623,766 (WO03079796) "Process for insecticidal formulation effective in controlling malarial vector, mosquitoes" (CSIR patent) wherein essential oil of Foeniculum vulgare and other plants from the family Apiaceae, Geraniaceae, Lamiaceae, Meliaceae, Myrtaceae, Poaceae, Zinziberaceae or mixtures thereof are taken. The essential oil may be extracted from Eucalyptus citridiora, Mentha spicata var. viridis, Mentha arvensis, Mentha piperata, Ocimum basilicum, Cymbopogon species, Pelargonium graveolens and/or Zinziber offlcinale or mixtures thereof. The essential oil of the referred patent is derived from Foeniculum vulgare varying from ours having entirely different components. The drawbacks are, it is effective against mosquito larvae of various species selected from the

group Aedes aegyptii, Anopheles stephensi, Anopheles quadrimaculatus and Culex spp. contrasting the present invention which is effective against all the groups of mosquitoes, bacteria, viruses and fungi.
Reference may be made to European Pat. No. EP0908171 which claims to have a herbal formulation for cleaning composition in the form of a dry powder containing a herb acting as a detergent (e.g. Bassia malabaricd), a herb acting as a foam booster (e.g. Cocos nuciferd), a herb acting as a foaming agent (e.g. Sapindus trifoliatus), a herb acting as a colouring agent (e.g. Hibiscus rosa sinesis) and a herb acting as a conditioner (e.g. Trigonella foenum graecum). Besides cleaning this also acts as an insecticide/insect repellent with a herb having antibiotic properties such as Melia azadirachta. The referred patent is completely herbal in nature. The drawbacks are, it is a cleaning composition rather than an insecticide, the elements of composition are also completely different, and the claim to act as an insecticide is entirely secondary.
Reference may be made to Chinese Pat. No. CN1451281 disclose a vegetative insecticide for killing cabage caterpillar, slug, snail, etc which is prepared from stemona root through soaking it in alcohol having high effect and no environmental pollution. The drawbacks are, that it is claimed to be effective against caterpillar, slug, and snail and has different elements of composition.
Reference may be made to Chinese Pat. No. CN1242947 the biological insecticide which integrates insecticide, repellent and antimold into one body, is easy to prepare, convenient for use, safe for human body, free from environmental pollution and has no adverse effects to books, paper and film, etc. and is made up by using (wt%) pyrethrins 10%, eucalyptus oil 3%, pine cone oil 5%, menthanol 2%, natural camphor 10%, Chinese herbal medicine powder 10%, diatomaceous earth 20%, paraffin wax 30% and Chinese prickly ash 10%. The drawbacks are that it is not completely herbal in nature and differs in one or more elements of composition and objectivity having insecticidal, repelling and antimold properties into one single component.
Reference may be made to Chinese Pat. No. CN1120890 is used in prevention and elimination of diseases and pests in vegetables, fruits, crops and other plants. It is prepared with traditional herbal medicinal material, surfactant, kerosene and water through lixiviation and mixing and has long-lasting effect and is innocuous to people and farm animals. The drawbacks are that it is composed of inorganic elements as surfactant and has different elements of composition. Reference may be made to Chinese Pat. No. CN 1081818 which is an inorganic insecticide for preventing and eliminating plant diseases and insect pests composed of Chinese herbal

medicines and features broad spectrum, high effect, low poison and low cost suitable for crops, vegetable, tree, fruit tree, flower and grass. The drawbacks are that it is not organic in nature, is not specific to mosquito, bacteria, virus and fungi, in-spite of having high spectrum of application and is composed of entirely different ingredients.
Reference may be made to Chinese Pat. No. CN 1076082 plant insecticide which has strong killing power to aphis, cabbage caterpillar, bollworm and other pests is made up of multiple Chinese herbal medicines, solvent, and emulsifier through crushing, extraction, concentration and proportioning. It features low consumption, broad spectrum in killing insects, long useful life, accelerating growth of crops, and very weak poison to people and animal. The drawback is that besides having different target of application it has altogether different ingredients and differs in objectivity.
Reference may be made to Chinese Pat. No. CN 1063601 which claims for a method for the preparation of highly efficient, low residue insecticide for agricultural purpose from traditional Chinese herbal plant and agricultural chemicals. It comprises of bittered bark liana, flavescent sophora, China berry, tobacco, datura flower or Chinese herbal medicines, golden ox-knee root, insect flower, arisaema tuber, castor oil as raw materials through breaking, soaking, boiling and concentration. The drawbacks are that it has altogether different composition and is mainly used in resisting insect pest, insecticide, and hastening the growth of plants with low toxicity for crops, forest, fruits and vegetables etc. contrasting the present formulation having applicability specific to mosquito, bacteria, virus and fungi.
Reference may be made to Chinese Pat. No. CN 1067783 disclose a synergistic agricultural insecticide saving fertilizers to the tune of 40%. The drawbacks are that it has high components of fertilizer and has low specificity.
Reference may be made to Chinese Pat. No. CN1059256 made from organic solvent extracts of hot pepper and toosendan fruit which relates to a farm insecticide curing various destructive insects of vegetables, fruit trees and crops which is effective, non-toxic and free from the public hazard. The drawbacks are it has no effective specificity against mosquito, bacteria, virus and fungi, has different constituents and is dissimilar in objectives. Reference may be made to Japanese Pat. No. JP56077212 claims to have an insecticide, including a Chinese herbal crude drugs Phellodendron amurense RUPRECHT, Artemisia princeps Pamp., etc., and is oxidized with potassium permanganate, benzoyl peroxide or bleaching powder to give an agricultural chemical or an insecticide having a fortified insecticidal action. A diluting agent, an emulsifier, a dispersing agent or a penetrant may be further added thereto, and the insecticidal action may be further fortified by the electrostatic

treatment. The drawbacks are that it is not herbal in nature and has few chemicals as
ingredient.
Reference may be made to Chinese Pat. No. CN 1397169 claims to have an insecticide in the
form of liquid, powder, or fumigant is prepared from eight Chinese-medicinal plants including
cnidium fruit, coptis root, tobacco leaf and datura flower. Its advantages are broadspectrum and
fewer residues. The drawbacks are that it is composed of different ingredient and has different
area of application in spite being herbal in nature.
All of the above patents in reference differs from the present formulation in principal constituent or in basic objectives or have chemicals as one of the ingredients posing consistent threat to environment. In the light of the above drawbacks a specific formulation was desired which is effective against mosquitoes, bacteria, virus and fungi, which is organic, non-toxic, non-polluting and easily digestible and degradable. With the known qualities of Cymbopogon winterianus as antibacterial, antibiotic, fungicide, mosquito repellent together with leaf extract of Lantana camara, Nicotiana tabacum, Tagetes erecta and Acacia catechu, Eucalyptus sps., Azadirachta indica, Ocimum kilimandscharicum (camphor Basil) and Vetiveria zizanioides which invigorates the insecticidal properties and effectiveness of the formulation. The developed formulation is antibiotic, antiviral, fungicide and is completely herbal in nature causing no persistent toxicity to soil and water, thus is easily degradable. Most of the hitherto known prior art even if they are herbal in nature vary in one or more of the principal element, constituents, objectives or method of use. In the light of the above there is scope of providing a herbal formulation acting as insecticide which is easier to use, is cheaper, degradable and is effective against mosquitoes, bacteria, virus and fungi.
Objects of the invention:
The main object of the present invention is to provide a herbal formulation comprising extracts of the plants selected from the group comprising of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus sp., Azadirachta indica, Ocimum kilimandscharicum, Vetiveria zizanioides, effective against mosquitoes, bacteria, virus and fungi or mixed population thereof.
Another object of the present invention is to provide a process for the preparation of the said herbal formulation.

Summary of the invention;
Accrodingly, the present invention provides a herbal formulation comprising extracts of the plants selected from the group comprising of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus sp., Azadirachta indica, Ocimum kilimandscharicum, Vetiveria zizanioides, optionally along with suitable diluents and additives, wherein the weight percentage is in the range of:
extract of Cymbopogon winterianus 16 - 24%,
extract of Lantana camara 11-15%,
extract of Nicotiana tabacum 12-18%,
extract of Tagetes erecta 1 - 12%,
extract of Acacia catechu 5 - 9%,
extract of Eucalyptus sp. 13-18%,
extract of Azadirachta indica 5 - 9%,
extract of Ocimum kilimandscharicum 5 - 9% and
extract of Vetiveria zizanioides 10-15%,
wherein the said weight percentages are percentages of the total weight of the composition.
In an embodiment of the invention, the said formulation is in the form of a liquid, spray etc.
In still an embodiment of the invention, the extracts are obtained from plant parts selected from the group consisting of leaf, rhizome, wood, seed or aerial parts.
In still another embodiment of the invention, the extract of Cymbopogon winterianus, Lantana camara, ,Nicotiana tabacum, Tagetes erecta, Eucalyptus spp. and Ocimum kilimandscharicum used are leaf extract.
In yet an embodiment of the invention, the extract of Azadirachta indica used is leaf and seed extract.
In yet another embodiment of the invention, the extract of Acacia catechu used is wood extract.
Further, in an embodiment of the invention, the extract of Vetiveria zizanioides is root extract.

In still another embodiment of the invention, the said formulation is effective against vector up to 98% within 5 hours of application.
In still another embodiment of the invention, the said vector is selected from the group consisting of mosquitoes, bacteria, viruses, fungi or mixed population thereof.
In yet an embodiment of the invention, the mosquito is preferably Culexpipiens.
Further, in an embodiment of the invention, bacteria is selected from the group comprising Xanthomonas sps., Pseudomonas sps., Erwinia sps.
In still another embodiment of the invention, the virus is selected from the plant viruses, Virioids of family Pospiviroidae, Avsunviroidae and Satellite viruses having both single and double stranded RNA exemplified by Potato virus I (Solarium virus), Potato virus X (Solarium virus I), Potato virus Y, Potato virus A (Solarium virus 3), Tomato mosaic virus, Potato yellow dwarf virus, Papaya leaf curl virus (Tobacco virus 16 or Nicotiana virus 10), Tobacco mosaic virus, Cucumber mosaic virus, Potato mild or latent mosaic virus, Potato vein bending virus, Hibiscus virus I, Banana virus I (Musa virus I), Phaseolus Virus I.
In yet another embodiment of the invention, the fungi is selected from the group comprising Helminthosporium sps., Pyricularia sps., Ustilago sps., Puccinia sps., Peronospora sps., Erysiphe sps., Fusarium sps., Sphaerotheca sps., Phytophthora sps., Alternaria sps., Mycospherellasps., Cercosporasps., Botrytis sps.
In still an embodiment of the invention, the process for preparation of the said herbal formulation comprising the following steps of:
(a) obtaining plant material of the plants selected from the group consisting of
Cymbopogon w interianus , Lantana camara, Nicotiana tabacum, Tagetes
erecta, Acacia catechu, Eucalyptus spp, Azadirachta indica, Ocimum
kilimandscharicum, Vetiveria zizanioides;
(b) drying the plant material of step (a) in shade;
(c) powdering the dried plant material of step (b) to obtain a coarse plant powder
material;

(d) extracting the powdered plant material of step (c) with aqueous alcohol at a
temperature up to 50°C to obtain an aqueous alcoholic extract;
(e) diluting the extract obtained from step (c) with distilled water up to 20% to
obtain the desired formulation.
In yet an embodiment of the invention, the alcohol used is ethanol.
In still another embodiment of the invention, the ratio of the water to alcohol is in the range of 80:20 to 85:15.
In yet another embodiment of the invention, the ratio of plant parts and aqueous alcohol used is in the range of 1:15 to 2:20.
Detailed description of the invention;
The formulation for herbal insecticide of the present invention is derived only from the natural ingredients such as leaf extract of Java citronella grass (Cymbopogon winterianus), Lantana (Lantana camara), tobacco (Nicotiana tabacum), African marigold (Tagetes erecta), Khair tree (Acacia catechu), Eucalyptus sps., Neem (Azadirachta indica), Camphor Basil (Ocimum kilimandscharicum) and Vetiver grass (Vetiveria zizanioides). It is an effective insecticide derived only from natural source. It is a novel product as it is very effective against mosquitoes, bacteria, viruses and fungi, includes no inorganic chemical ingredients so as to cause pollution to nearby water and soil, is easily digestible eliminating the dangers of further toxicity, is easy to use and make and is cheap.
The fresh / aqueous /ethanolic extracts is derived from plant extract of Java citronella, Lantana, Tobacco, African marigold, Arecanut palm, Neem, Ocimum and Vetiver grass are thoroughly mixed to prepare the mother liquor. It is then diluted to 20 % for commercial grade product which is very effective against mosquito, bacteria and virus. It assimilates the effective properties of Java citronella, Lantana, Tobacco, African marigold, Arecanut palm, Neem, Ocimum and Vetiver grass. The properties are derived from citronellal, citronellol, citronellic acid and hydroxyl citronellic acid present in Cymbopogon winterianus, Umuhengerin (effective against Staphylococcus aureus, Salmonella typhimurium, Candida tropicalis, Aspergillus niger, A.fumigatus, Trichophyton mentagrophytes and Microsporium canis) found in Lantana camara, nicotine in Nicotiana tabacum, thiophenes (antibacterial and insecticidal),

ocimenones (anti-fungal and larvicidal), dihydrotagetone, tagetones, piperitone (strongly antibacterial) found in Tagetes erecta. a, P and Y-catechin, catechutanic acid, tannins, 1-epicatechin, taxifolin (antibacterial, antifungal, antiviral, anti-inflammatory) present in Acacia catechu, tannins and citronellol present in Eucalyptus sps., azadirachtin and triterpenoids 6 alpha-O-acetyl-7-deacetylnimocinol and meliacinol present in Azadirachta indica, camphor and 1,8-cineole present in Ocimum kilimandscharicum and vetiverol, vitivone, terpenes such as vetivenes present in Vetiveria zizanioides which acts as antiseptic.

Fresh / Aqueous Extracts

Natural Extracts in Ethanol

Solution
Dilution
Packaging
Ready for Use
The formulation for herbal insecticide of the present invention is derived only from the natural ingredients such as plant extracts of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus sps., Azadirachta indica, Ocimum kilimandscharicum and Vetiveria zizanioides. It utilizes the effective properties of Cymbopogon winterianus (Java citronella), Lantana camara, Nicotiana tabacum, Tagetes erecta, Acacia catechu, Eucalyptus sps., Azadirachta indica, Ocimum kilimandscharicum and Vetiveria zizanioides and is most effective against mosquitoes, bacteria, viruses and fungi. The properties are derived from citronellal, citronellol, citronellic acid and hydroxyl citronellic acid present in Cymbopogon winterianus, Umuhengerin (0.2 mg/ml) effective against Staphylococcus aureus, Salmonella typhimurium, Candida tropicalis, Aspergillus niger, Aspergillus fumigatus, Trichophyton mentagrophytes and Microsporium canis (J. Nat. Products, 1988) present in Lantana camara, nicotine in Nicotiana tabacum, thiophenes known for antibacterial and insecticidal properties present in Tagetes erecta. Some typical constituents

of Tagetes sps. oils are reported to have specific properties i.e. ocimenones show anti-fungal and larvicidal activity and dihydrotagetone, tagetones and piperitone possess a strong inhibition activity against the growth of gram positive and gram negative bacteria. Catechin, catechutanic acid, tannins (Proc. Chem. Soc. Lond., 1902, 139; 1904,171; 1905, 398). Wood contains a, ft and Y-catechin (J. Indian Chem. Soc., 1930, 279; 1931, 143); l-epicatechin (J. Sci. industr. Res., 1948, 59B), taxifolin has antibacterial, antifungal, antiviral, anti-inflamatory and anti-oxidant properties (Harbone, J.B., et. al. 1999. Phytochemistry dictionary, Tailor and Francis Ltd., London) present in Acacia catechu, tannins and citronellol present in Eucalyptus sps., azadirachtin and triterpenoids 6 alpha-O-acetyl-7-deacetylnimocinol and meliacinol present in Azadirachta indica, camphor and 1,8-cineole present in Ocimum kilimandscharicum and vetiverol, vitivone, terpenes such as vetivenes from Vetiveria zizanioides which acts as antiseptic
Tagetes erecta flowers contain quercetagetin (J. Indian Chem. Soc., 1938, 87); quarcetagitrin, a glucoside of quercetagetin is isolated from dried flowers (Proc. Indian Acad. Sci., vol.HA, 1941, 289; Chem. Abstr., 1942, 2555). Leaves and flowers yield essential oils (0.3 and 0.34%) respectively, contains a-and /?-pinenes, dipentene, menthol and geraniol (Indian perfum. 1980, 24,163; Chem. Abstr., 1981,94,145159 j); leaf essential oil contained linalool (26.80), (+) limonene (19.67), tagetone (13.39), /?-ocimene (7.61)/5-phellandrene (4.97) and linalyl acetate (3.56%), a-pinene and nonanal (J. Indian Chem. Soc., 1981, 58,104); quercetagetin and quarcetagitrin is also isolated from seeds (Khim. Prir. Soedin. 1983,104; Chem. Abstr., 1983, 98, 194981 n); 8-hydroxyquercetagetin obtained from flowers (Proc. Indian Natl Sci. Acad. 1983, 49A, 408); kaempferol, its 7-0-rhamnoside and kaempferitrin isolated from leaves; 6-hydroxykaempferol-7-O-gIucoside, quercetagetin and quercetagetrin from flowers (Fitoterapia 1983, 549) (Ref. Rastogi and Mehrotra ed. Compedium of Indian Medicinal plants; 3;623;1993). Extract of the flowers are used in bleeding piles, unhealthy ulcers while leaf, flower and seed extract is used in boils and wounds. The essential oils exhibited antimicrobial activity against fungi, gram-positive and gram-negative micro-organisms (Flavour Fragrance J. 1986, 1, 169. Chem. Abstr. 1987,107,161480z; Herba Hung. 1987, 26, 49; Chem Astr. 1987, 107, 151202w).
The fresh / aqueous /ethanolic extracts of the above plants were prepared and thoroughly mixed and the resulting solution was diluted to 20%. This is ready for use as an insecticide, which was tested to be effective against mosquito, bacteria, virus and fungi. It killed the above vector within 1 hr of application and 98% of lethality was tested within 5 hrs. of application.
The novel formulation of the present invention provides for herbal insecticide having a composition which is not a mere admixture but a synergistic mixture having properties which are distinct and different from mere aggregation of the properties of the individual ingredients. The present formulation is completely herbal in nature, being easily digestible by the ambient environment, is effective against mosquito, bacteria, viruses and fungi, has no observed toxicity to vegetation, is completely devoid of inorganic chemicals and is economically viable. The present herbal formulation obliterates the side effects of inorganic chemicals and the resultant occurrence of diseases such as cancer, etc., due to presence of toxicants in the cereals, fruits and vegetables.
The novelty of providing a totally herbal insecticide has been realized by the non-obvious inventive steps of providing a synergistic mixture of the ingredients constituting the formulation for herbal insecticide of the present invention.
The following examples are given by way of illustration of the present invention and should not be construed to limit the scope of present invention.
Example 1
Cymbopogon winterianus (Java citronella) 22.76 %
Lantana camara (leaf extract) 13.32%
Nicotiana tabacum 15.51%
Tagetes erecta 7.66 %
Acacia catechu 5.46%
Eucalyptus spp. 14.22 %
Azadirachta indica 5.46%
Ocimum kilimandscharicum 5.17%
Vetiveria zizanioides 10.44 %
The aqueous/fresh extracts were prepared individually and they were thoroughly mixed with the ethanolic extracts. 'The resulting solution was ready for use. The present formulation is an effective insecticide but has more pronounced impact on mosquito and viruses.
11

Example 2
Cymbopogonwinterianus (Java citronella) 16.22%
Lantana camara (leaf extract) 14.32 %
Nicotiana tabacum 16.77%
Tagetes erecta 9.15%
Acacia catechu 5.83%
Eucalyptus spp. 14.24%
Azadirachta indica 6.61 %
Ocimum kilimandscharicum 5.58 %
Vetiveria zizanioides 11.28 %
This formulation is found to be effective against mosquito, bacteria and virus.
Example 3
Cymbopogon \vinterianus (Java citronella) 17.78%
Lantana camara (leaf extract) 11.11 %
Nicotiana tabacum 13.33%
Tagetes erecta 11.11%
Acacia catechu 8.89 %
Eucalyptus spp. 13.33%
Azadirachta indica 7.67 %
Ocimum kilimandscharicum 6.67 %
Vetiveria zizanioides 10.11 %
This is the normal formulation for insecticide, found to be effective against mosquito, bacteria virus and fungi.
It is the combination of plant extracts and natural organic compounds. The disclosed details reveal the need of a product which is completely natural to spray and is effective against mosquito, bacteria and virus. Although, many herbal insecticides which are mostly Chinese are likely to hit the market sooner or later, our product provides an effective organic insecticide effective against mosquito, bacteria, viruses and fungi and is eco-friendly in nature without

causing any toxicity to the ambient environment. It killed the vector within 1 hr of application and 98% of lethality was tested within 5 hrs. of application.
Advantages of the present invention:
The main advantages of the present invention are:
1. The present formulation is herbal in nature with no inorganic chemicals so as to cause
any toxicity to nearby soil and water bodies. It is completely natural and is bio
degradable.
2. It has no observed toxicity against vegetation.
3. It is effective against mosquito, bacteria, virus and fungi.
4. It is easy to make and use.
5. It is very cheap and is within the reach of the general customer.

We claim;
1. An herbal formulation comprising extracts of the plants selected from the group
comprising of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum,
Tagetes erecta, Acacia catechu, Eucalyptus sp., Azadirachta indica, Ocimum
kilimandscharicum, Vetiveria zizanioides, optionally along with suitable diluents and
additives, wherein the weight percentage is in the range of:
extract of Cymbopogon \vinterianus 16-24%,
extract of Lantana camara 11-15%,
extract of Nicotiana tabacum 12-18%,
extract of Tagetes erecta 7 - 12%,
extract of Acacia catechu 5 - 9%,
extract of Eucalyptus sp. 13 - 18%,
extract of Azadirachta indica 5 - 9%,
extract of Ocimum kilimandscharicum 5 - 9% and
extract of Vetiveria zizanioides 10-15%,
wherein the said weight percentages are percentages in the total weight of the composition.
2. A formulation as claimed in claim 1, wherein the said formulation is in the form of a
liquid, spray etc.
3. A formulation as claimed in claim 1, wherein the extracts are obtained from plant
parts selected from the group consisting of leaf, rhizome, wood, seed or aerial parts.
4. A formulation as claimed in claim 1, wherein the extract of Cymbopogon winterianus,
Lantana camara, .Nicotiana tabacum, Tagetes erecta, Eucalyptus spp. and Ocimum
kilimandscharicum used are leaf extract.
5. A formulation as claimed in claim 1, wherein the extract of Azadirachta indica used is
leaf and seed extract.
6. A formulation as claimed in claim 1, wherein the extract of Acacia catechu used is
wood extract.
7. A formulation as claimed in claim 1, wherein the extract of Vetiveria zizanioides is
root extract.
8. A formulation as claimed in claim 1, wherein the said formulation is effective against
vector up to 98% within 5 hours of application.

9. A herbal formulation as claimed in claim 8, wherein the said vector is selected from
the group consisting of mosquitoes, bacteria, viruses, fungi or mixed population
thereof.
10. A herbal composition as claimed in claim 9, wherein the mosquito is preferably Culex
pipiens (common mosquito).
11. A herbal formulation as claimed in claim 9, wherein bacteria is selected from the
group comprising Xanthomonas sps., Pseudomonas sps., Erwinia sps.
12. A composition as claimed in claim 9, wherein the virus is selected from the plant
viruses, Virioids of family Pospiviroidae, Avsunviroidae and Satellite viruses having
both single and double stranded RNA exemplified by Potato virus I (Solanum virus),
Potato virus X (Solanum virus I), Potato virus Y, Potato virus A (Solanum virus 3),
Tomato mosaic virus, Potato yellow dwarf virus, Papaya leaf curl virus (Tobacco
virus 16 or Nicotiana virus 10), Tobacco mosaic virus, Cucumber mosaic virus, Potato
mild or latent mosaic virus, Potato vein bending virus, Hibiscus virus I, Banana virus I
(Musa virus I), Phaseolus Virus I.
13. A composition as claimed in claim 9, wherein the fungi is selected from the group
comprising Helminthosporium sps., Pyricularia sps., Ustilago sps., Puccinia sps.,
Peronospora sps., Erysiphe sps., Fusarium sps., Sphaerothecasps., Phytophthorasps.,
Alternaria sps., Mycospherella sps., Cercospora sps., Botrytis sps.
14. A process for the preparation of an insecticidal herbal formulation of claim 1, wherein
the said process comprising the steps of:

(a) obtaining plant material of the plants selected from the group consisting of
Cymbopogon winterianus , Lantana camara, Nicotiana tabacum, Tagetes
erecta, Acacia catechu, Eucalyptus spp, Azadirachta indica, Ocimum
kilimandscharicum, Vetiveria zizanioides;
(b) drying the plant material of step (a) in shade;
(c) powdering the dried plant material of step (b) to obtain a coarse plant powder
material;
(d) extracting the powdered plant material of step (c) with aqueous alcohol at a
temperature up to 50°C to obtain an aqueous alcoholic extract;
(e) diluting the extract obtained from step (c) with distilled water up to 20% to
obtain the desired formulation.
15. A process as claimed in claim 14, wherein the plant material used is selected from the group consisting of leaf, rhizome, wood, seed or aerial parts.

16. A formulation as claimed in claim 14, wherein the alcohol used is ethanol.
17. A process as claimed in claim 14, wherein the ratio of the water to alcohol is in the
range of 80:20 to 85:15.
18. A process as claimed in claim 14, wherein the ratio of plant parts and aqueous alcohol
used is in the range of 1:15 to 2:20.
19. An herbal formulation comprising extracts of the plants selected from the group
comprising of Cymbopogon winterianus, Lantana camara, Nicotiana tabacum,
Tagetes erecta, Acacia catechu, Eucalyptus sp., Azadirachta indica, Ocimum
kilimandscharicum, Vetiveria zizanioides and a process for the preparation thereof
substantially as herein described with reference to the examples and drawings
accompanying this specification.

Documents

Name Date
921-del-2006-form-18-(31-01-2008).pdf 2008-01-31
921-del-2006-correspondence-others-(31-01-2008).pdf 2008-01-31
921-del-2006-form-5.pdf 2011-08-20
921-del-2006-form-3.pdf 2011-08-20
921-del-2006-form-2.pdf 2011-08-20
921-del-2006-form-1.pdf 2011-08-20
921-del-2006-description (provisional).pdf 2011-08-20
921-del-2006-description (complete).pdf 2011-08-20
921-del-2006-abstract.pdf 2011-08-20
921-del-2006-correspondence-others.pdf 2011-08-20
921-del-2006-claims.pdf 2011-08-20
921-DEL-2006_EXAMREPORT.pdf 2016-06-30
921-DEL-2006-1.PDF 2017-11-23
921-DEL-2006-HearingNoticeLetter.pdf 2017-12-01

Orders

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