Phytoremediation Method For Degraded Spaces Using Genetically Modified Vegetal Species

Abstract

Abstract: The invention is related to the search, selection and genetic modification of wild vegetal species; moreover it consists in defining the effect of the introduction of various genes on the phytoremediation properties of the model vegetal species, Populus tremula x tremuloides cv. Etropole proposed for wet grounds, and Nicotiana glauca for dry grounds. Starting species used for genetic modification are wild type (wt) species, selected among specific contaminated spaces. Genetic modification of Populus tremula x tremuloides cv. Etropole species consists in the introduction of TAPCS1 and AtPCSI genes, and YCF gene in a second step. The genetic modification of Nicotiana glauca consists in the introduction of TaPCSI and YCF gens; or the TaPCSI, YCF1 and CBP4 genes. Modifications enhanced the biomass production; and at the same time the tolerance to heavy and radioactive elements of different toxicity; to industrial and domestic detergents; it increased the tolerance to spillage of crude oils and hydrocarbon solvents, or light, medium or heavy oil refining products; enhanced phytoremediation of spaces contaminated with sodium ions in conditions of salty waters, infiltrations of sea water in soils; proposed species enhanced the tolerance to vegetal diseases; and to low humidity and high environmental temperatures of desert spaces.

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