DESC:FIELD OF THE INVENTION
The present invention relates to improved agrochemical combinations for the control of soil borne and seed borne diseases and pests. More particularly, the present invention relates to a pesticidal composition comprising of a) pyraclostrobin, b) thiophanate-methyl, c) thiamethoxam and their compositions for the treatment of plant propagation materials.
BACKGROUND OF THE INVENTION
Pest, disease, and weeds are the frightening trio which is responsible for the considerable number of losses in agriculture. Besides these usual suspects, rodents, nematodes, plant parasites, and snails have also been able to impose enormous losses to the crops. The damage would run to alarming numbers where the management strategies are not adopted suitably.
Hence, responsible crop production and management requires adoption and implementation of practices that critically address these issues of crop loss. Therefore, crop protection practices have become an important aspect of agriculture. Usage of fungicides as chemical means of controlling crop diseases pertains to be the utmost priority for reducing yield losses under favorable conditions. With the increase in adoption of number of fungicides in the current agricultural practices, the pathogen population exposed to the repeated use of same fungicides develops resistance. Hence it results in the poor management of disease and opts for alternative measures to reduce the resistance development in pathogen population. Thus, monitoring the resistance development in pathogen seems an important context to have effective control of the disease by efficient fungicides.
Combinations of fungicides and insecticides are often used to broaden the spectrum of control of insect and fungal pests, and to reduce dosages, thereby reducing environmental impact, and decreasing the chances of developing resistance. The combination of fungicides and insecticides at times demonstrates an additive or synergistic effect that results in an improved control of the pests.

Damage to seeds from insects and fungal pests is a major concern for the agriculturalist. There are various diseases such as seed rot which reduce germination rates and cause considerable decrease in yield. Treating the seeds with fungicides and/or insecticides can help reduce damage caused by soil pests. Additional potential advantages of treating seeds or other plant propagation materials are improvements in germination rates, increased yield, and improved plant health.
WO2006069654A2 discloses novel active substance combinations containing an active substance selected from neonicotinoid clothianidin, imidacloprid, thiacloprid, dinotefuran, acetamiprid, nitenpyram and thiamethoxam series and at least one type of active substance selected from a strobilurin fungicide, picoxystrobin, pyraclostrobin, dimoxystrobin, metominostrobin and orysastrobin series.
The mixing of insecticides with fungicides results in incompatibility of physical nature and may alter efficacy of the active ingredients. Hence, it requires due trial and experimentation to assess the compatibility of fungicides with insecticides and their influence on crops. Many pesticides with combination of one or more actives have been used by farmers. But still there is a requirement for new combinations which can provide efficacy, low-cost benefit ratio, broad spectrum protection and decreased environmental load.

OBJECT OF THE INVENTION
One objective of the present invention is to provide an improved agrochemical combination of fungicides and insecticides for the control of soil borne and seed borne diseases in a wide range of crops but not limited to soybean, ground nut, and corn crops.
An object of the present invention is to provide a seed treatment composition comprising of a) pyraclostrobin present in a range from 1% (w/w) to 5% (w/w), b) thiophanate-methyl present in a range from 8% (w/w) to 16% (w/w), c) thiamethoxam present in a range from 20% (w/w) to 35% (w/w), and suitable agrochemical additives.
Another object of the present invention is to provide seed treatment composition formulated as a flowable concentrate for seed treatment (FS) and water dispersible powder for slurry seed treatment (WS).
Another objective of the present invention is to provide a method and a composition for controlling insect pests and fungal diseases by treating plant propagation material.
Yet another objective of the present invention is to provide improved agrochemical combinations of fungicides and insecticides that promote plant health.

SUMMARY OF THE INVENTION
The present invention aims to provide an improved agrochemical combination of fungicides and insecticides for the control of soil borne and seed borne diseases in crops but not limited to soyabean, ground nut, and corn crops.
In an aspect of the present invention, a seed treatment composition is provided comprising of a) pyraclostrobin present in a range from 1% (w/w) to 5% (w/w), b) thiophanate-methyl present in a range from 8% (w/w) to 16% (w/w), c) thiamethoxam present in a range from 20% (w/w) to 35% (w/w), and suitable agrochemical additives.
In an aspect of the present invention, suitable agrochemical additives are selected from a wetting agent, a dispersing agent, an anti-freezing agent, a biocide, a defoamer, a rheology modifier, a binder, a pigment, a co-filler, a filler and combination thereof.
In an aspect of the present invention, the pesticidal composition comprises the wetting agent selected from the group comprising polyoxyethylene polyoxy propylene condensate, alcohol ethoxylates, sodium alkyl naphthalene sulphonate blend, and sodium isopropyl naphthalene sulfonate, present in a range from 1% to 5% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the dispersing agent selected from the group comprising tristyryl phenyl ether phosphate TEA salt, acrylic co-polymer, tristyryl phenol ethoxylates, sodium ligno sulphonates, alkyl naphthalene sulphonates formaldehyde condensate, sodium naphthalene sulphonate blend, and combination thereof, present in a range from 1% to 10% (w/w).
In an aspect of the present invention, the pesticidal composition comprises an anti-freezing agent propylene glycol, present in a range from 2% to 10% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the rheology modifiers xanthan gum present in a range from 1% to 15% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the binder selected from vinyl acetate copolymer, vinyl pyrrolidone, and poly vinyl pyrrolidine, present in a range from 0.5% to 3.0% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the pigment selected from naphthol AS-based pigments, Permanent Red FGR 03, and Permanent Red FGR 112, present in a range from 0.5% to 4.0% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the biocide 1, 2-Benzisothiazol-3(2H)-one, present in a range from 0.05% to 0.5% (w/w).
In an aspect of the present invention, the pesticidal composition comprises the defoamer selected from silicon powder, and silicone emulsion, present in a range from 0.5% to 2.0% (w/w).
In an aspect of the present invention, the pesticidal composition comprises co-filler silicon dioxide, present in a range from 2% to 5% (w/w).
In an aspect of the present invention, the pesticidal composition comprises filler selected from distilled water, and china clay, present in Q.S.
In an aspect of the present invention, the seed treatment composition is formulated as a flowable concentrate for seed treatment (FS) and water dispersible powder for slurry seed treatment (WS).
In yet another aspect of the present invention, a process is provided for preparing a flowable concentrates for seed treatment (FS) composition comprising of a) pyraclostrobin, b) thiophanate-methyl and c) thiamethoxam.
In yet another aspect the present invention provides a process for preparing a water dispersible powder for slurry seed treatment (WS) composition comprising of a) pyraclostrobin, b) thiophanate-methyl and c) thiamethoxam.

DESCRIPTION OF THE INVENTION
The following detailed description is presented to enable any person skilled in the art to make and use the invention. For purposes of explanation, specific nomenclature is set forth to provide a thorough understanding of the present application. However, it will be apparent to one skilled in the art that these specific details are not required to practice the invention. Descriptions of specific applications are provided only as representative examples. The present application is not intended to be limited to the embodiments shown but is to be accorded the widest possible scope consistent with the principles and features disclosed herein.
It is to be noted that, as used in the specification, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
Similarly, the words "comprise," "comprises," and "comprising" are to be interpreted inclusively rather than exclusively. Likewise, the terms "include," "including" and "or" should all be construed to be inclusive, unless such a construction is clearly prohibited from the context. However, the embodiments provided by the present disclosure may lack any element that is not specifically disclosed herein. Thus, a disclosure of an embodiment defined using the term "comprising" is also a disclosure of embodiments "consisting essentially of” and "consisting of” the disclosed components. Where used herein, the term "example" particularly when followed by a listing of terms, is merely exemplary and illustrative, and should not be deemed to be exclusive or comprehensive. Any embodiment disclosed herein can be combined with any other embodiment disclosed herein unless explicitly indicated otherwise.
The expression of various quantities in terms of “% w/w” or “%” means the percentage by weight, relative to the weight of the total solution or composition unless otherwise specified.
The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control of fungi, insects-pests or disease.
As used herein, the term "effective amount" means the amount of the active substances in the compositions to achieve an observable effect on growth, including the effects of necrosis, death, retardation, prevention, removal, destruction, or otherwise diminishing the occurrence and activity of the target organism. An effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like.
As used herein, the terms "crops" and "vegetation" can include, for instance, dormant seeds, germinant seeds, emerging seedlings, plants emerging from vegetative propagules, immature vegetation, and established vegetation.
As used herein, immature vegetation may be understood to include small vegetative plants prior to reproductive stage, and mature vegetation may be understood to include vegetative plants during and after the reproductive stage.
The present invention aims to provide an improved agrochemical combination of fungicides and insecticides for the control of soil borne and seed borne diseases in soybean. Preferably, a combination of pyraclostrobin, thiophanate-methyl and thiamethoxam.
Pyraclostrobin is a quinone outside inhibitor-type fungicide used in agriculture. Among the Q?Is, it lies within the strobilurin chemical class. It has been widely employed to control plant diseases by inhibiting the mitochondrial respiration of pathogenic fungi.
Pyraclostrobin is a carbamate ester chemically known as the methyl ester of [2-({[1-(4-chlorophenyl)-1H-pyrazol-3-yl] oxy} methyl) phenyl] methoxy carbamic acid and has the following structure:

Thiophanate-methyl fungicide, is widely used to control some of the most common fungal diseases in crops, is metabolized in animals into benzimidazole compounds, including the reproductive toxicant carbendazim. However, standard toxicological tests did not indicate that thiophanate-methyl may cause testicular toxicity and/or embryotoxicity, which are typical effects of many benzimidazoles.
Thiophanate-methyl is chemically known as dimethyl N, N'- [1,2-phenylenebis (azanediyl carbonothioyl)] dicarbamate and has the following structure:

Thiamethoxam is a broad-spectrum, systemic insecticide, which means it is absorbed quickly by plants and transported to all its parts, including pollen, where it acts to deter insect feeding.
Thiamethoxam is chemically known as, 3-[(2-Chloro-5-thiazolyl) methyl] tetrahydro-5-methyl-N-nitro-4H-1,3,5-oxadiazin-4-imine and has the following structure:

In a preferred embodiment, the present invention provides an improved agrochemical combination of fungicides and insecticides for the control of soil borne and seed borne diseases in soybean, ground nut, and corn crops.
In an embodiment the present invention provides a seed treatment composition comprising of a) pyraclostrobin present in a range from 1% (w/w) to 5% (w/w), b) thiophanate-methyl present in a range from 8% (w/w) to 16% (w/w), c) thiamethoxam present in a range from 20% (w/w) to 35% (w/w), and suitable agrochemical additives.
In an embodiment of the present invention, the seed treatment composition is formulated as a flowable concentrate for seed treatment (FS) and water dispersible powder for slurry seed treatment (WS).
In another embodiment, the compositions of the present invention may typically be produced by mixing the actives in the composition with an inert carrier and adding surfactants and other excipients and carriers as needed for various formulations. During application, a common excipient can be mixed with the composition.
Suitable additives may be solid or liquid and are generally a substance commonly used in formulation processing, for example, a wetting agent, a dispersing agent, an anti-freezing agent, a biocide, a defoamer, a rheology modifier, a binder, a pigment, a co-filler, a filler and combination thereof.
The pesticidal composition of the present invention may be formulated as Granular composition (GR), Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC), a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW). Preferably, the composition of the present invention is formulated as a flowable concentrate for seed treatment (FS) and water dispersible powder for slurry seed treatment (WS).
In addition, by treating soil with the pesticidal composition of the present invention, it becomes possible to simultaneously control pests inhabiting the soil and pests inhabiting the ground. In addition, in this method, since the dosage and the number of applications of the active ingredients are reduced and the effect is long-lasting effect, it is possible to provide a comprehensive control method.
A wetting agent is a substance that when added to a liquid increases the spreading or penetration power of the liquid by reducing the interfacial tension between the liquid and the surface on which it is spreading.
Accordingly, the composition of the present invention contains a wetting agent selected from the group comprising of polyoxyethylene polyoxy propylene condensate, alcohol ethoxylates, sodium alkyl naphthalene sulphonate blend, and sodium isopropyl naphthalene sulfonate, present in a range from 1% to 5% (w/w).
A dispersant or a dispersing agent is a substance which absorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersants are added to agrochemical compositions to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into water in a spray tank.
Accordingly, the composition of the present invention contains a dispersing agent selected from the group comprising of tristyryl phenyl ether phosphate TEA salt, acrylic co-polymer, tristyryl phenol ethoxylates, sodium ligno sulphonates, alkyl naphthalene sulphonates formaldehyde condensate, sodium naphthalene sulphonate blend, and combination thereof, present in a range from 1% to 10% (w/w).
The alkyl naphthalene sulfonates used in the compositions preferably have alkyl groups with 1 to 10 carbon atoms, such as methyl, isopropyl, n-butyl, sec-butyl, and nonyl. Especially preferred products are sodium butyl naphthalene sulfonate and sodium nonyl naphthalene sulfonate.
An anti-freezing agent is defined as an additive that, when added to a water-based fluid, will reduce the freezing point of the mixture. The suitable anti-freezing agent for the purpose of the present invention is propylene glycol, present in a range from 2% to 10% (w/w).
Rheology modifiers are designed to bring superior structuring to formulations, allowing to control the viscosity and are easy to use and provide better stability, and suspensibility performance. Flowable formulations require a rheology modifier to prevent sedimentation phenomena. A rheological modifier suitable for use in the present invention is Xanthan gum present in a range from 1% to 15% (w/w).
The binders suitable for the purpose of the present invention are vinyl acetate copolymer, vinyl pyrrolidone, and poly vinyl pyrrolidine, present in a range from 0.5% to 3.0% (w/w).
The pigment suitable for the purpose of the present invention is naphthol AS-based pigments, Permanent Red FGR 03, chemically known as [(4E)-N-(2-methylphenyl)-3-oxo-4-[(2,4,5-trichlorophenyl) hydrazinylidene] naphthalene-2-carboxamide 3-hydroxy-n-(2-methylphenyl)-4-((2,4,5-trichlorophenyl)azo)naphthalene-2-carboxamide)], and Permanent Red FGR 112, chemically known as [(4E)-N-(2-methylphenyl)-3-oxo-4-[(2,4,5-trichlorophenyl) hydrazinylidene] naphthalene-2-carboxamide 3-hydroxy-n-(2-methylphenyl)-4-((2,4,5-trichlorophenyl)azo) naphthalene-2-carboxamide)], present in a range from 0.5% to 4.0% (w/w).
The biocide suitable for the purpose of the present invention is 1, 2-Benzisothiazol-3(2H)-one, present in a range from 0.05% to 0.5% (w/w).
Defoamer is a chemical reagent developed for defoaming and foam suppression based on the principle of foaming. The defoamer suitable for the purpose of the present invention is silicon powder, and silicone emulsion, present in a range from 0.5% to 2.0% (w/w).
A co-filler suitable for the purpose of the present invention is silicon dioxide, present in a range from 2% to 5% (w/w).

A filler refers to solid chemicals that are added to a pesticide formulation to aid in the delivery of the active ingredient. Accordingly, the composition of the present invention contains a filler selected from distilled water, and china clay, present in Q.S.
In yet another embodiment the present invention provides a process for preparing a flowable concentrates for seed treatment (FS) composition of a) pyraclostrobin, b) thiophanate-methyl and c) thiamethoxam.
In yet another embodiment the present invention provides a process for preparing a water dispersible powder for slurry seed treatment (WS) composition of a) pyraclostrobin, b) thiophanate-methyl and c) thiamethoxam.
The composition of the present invention can also be applied in conjunction with one or more herbicides, fungicides, insecticides, nematicides, growth factor enhancers and/or one or more fertilizers.
The composition of the present invention may be applied by one of the methods selected from atomization, spreading, dusting, spraying, diffusion, immersion, irrigation, injection, mixing, sprinkling (water immersion), foaming, dressing, coating, blasting, and fumigation.
The present invention is further illustrated by the following examples which are provided merely to be exemplary of the inventions and are not intended to limit the scope of the invention. Certain modifications and equivalents will be apparent to those skilled in the art and are intended to be included within the scope of the present invention.

Examples:

Example 1: Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% (FS)
S. No. Composition Function Charge in (%)
1 Pyraclostrobin (Technical) Active ingredient 2.5
2 Thiophanate-methyl (Technical) Active ingredient 12.0
3 Thiamethoxam (Technical) Active ingredient 25.0
4 Polyoxyethylene polyoxy propylene condensate Wetting agent 2.0
5 Tristyryl phenyl ether phosphate TEA salt Dispersing agent 2.0
6 Propylene glycol Anti-freezing agent 10.0
7 1, 2-Benzisothiazol-3(2H)-one Biocide 0.05
8 Silicone emulsion Defoamer 0.50
9 Xanthan gum Rheology modifier 10.0
10 Vinyl pyrrolidone Binder 0.50
11 Naphthol AS pigment
(Permanent Red FGR 03) Pigment 2.0
12 Distilled Water Filler Q.S.

Procedure of preparation of Pyraclostrobin 2.5% + Thiophanate Methyl 12% + Thiamethoxam 25% (FS):
Step 1: According to the batch size, raw materials were mixed in following order to form a slurry
? Filler
? Biocide
? Anti-freezing agent
? Defoamer
? Wetting agent
? Dispersing agent
? Pyraclostrobin technical
? Thiophanate Methyl technical
? Thiamethoxam technical
? Pigment
? Binder
Step 2: Pre-mixing the slurry in a mixing chamber.
Step 3: The slurry is milled through bead mill instrument.
Step 4: Collecting the wet milled sample and washing the bead mill with balance amount of distilled water and transfer it to sample. Check input verses output. Check particle size of milled slurry. If it is as per specification (d50 value >5µ) then material is transferred to mixing chamber and mixing is started.
Step 5: Preparing a 2% xanthan gum solution in another high-speed shear mixture.
Step 6: Adding 2% xanthan gum solution to the above milled slurry under stirring condition as
per output received. Continue stirring for 1 hour and then send the sample for analysis.
Step 7: Packing the material in a suitable bottle.

Example 2: Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% (FS)
S. No. Composition Function Charge in (%)
1. Pyraclostrobin (Technical) Active ingredient 2.5
2. Thiophanate-methyl (Technical) Active ingredient 12.0
3. Thiamethoxam (Technical) Active ingredient 25.0
4. Alcohol ethoxylates Wetting agent 2.5
5. Acrylic copolymer Dispersing agent 3.0
6. Propylene glycol Anti-freezing agent 5.0
7. 1, 2-Benzisothiazol-3(2H)-one Biocide 0.1
8. Silicone emulsion Defoamer 0.5
9. Xanthan gum Rheology modifier 15.0
10. Vinyl acetate copolymer Binder 1.5
11. Naphthol AS Pigment 2.0
12. Distilled Water Filler Q.S.

The manufacturing procedure for composition of Example 2 is same as the one described in Example 1.
Example 3: Pyraclostrobin 5% + Thiophanate methyl 16% + Thiamethoxam 35% (FS)
S. No. Composition Function Charge in (%)
1. Pyraclostrobin (Technical) Active ingredient 5.0
2. Thiophanate-methyl (Technical) Active ingredient 16.0
3. Thiamethoxam (Technical) Active ingredient 35.0
4. Sodium alkyl naphthalene sulphonate blend Wetting agent 3.0
5. Tristyryl phenol ethoxylates TEA salt Dispersing agent 3.0
6. Propylene glycol Anti-freezing agent 5.0
7. 1, 2-Benzisothiazol-3(2H)-one Biocide 0.05
8. Silicone emulsion Defoamer 1.0
9. Xanthan gum Rheology modifier 5.0
10. Polyvinyl pyrrolidone Binder 0.5
11. Naphthol AS pigment
(Permanent Red FGR 112) Pigment 0.5
12. Distilled Water Filler Q.S.

The manufacturing procedure of composition of Example 3 is similar to the one described in Example 1.

Example 4: Pyraclostrobin 1% + Thiophanate methyl 8% + Thiamethoxam 20% (FS)
S. No. Composition Function Charge in (%)
1. Pyraclostrobin (Technical) Active ingredient 1.0
2. Thiophanate-methyl (Technical) Active ingredient 8.0
3. Thiamethoxam (Technical) Active ingredient 20.0
4. Sodium ligno sulphonates Dispersing Agent 5.0
5. Sodium alkyl naphthalene sulphonate blend Wetting agent 5.0
6. Silicone emulsion Defoamer 1.0
7. 1, 2-Benzisothiazol-3(2H)-one Biocide 0.1
8. Polyvinyl pyrrolidone Binder 2.0
9. Xanthan gum Rheology Modifier 10.0
10. Propylene glycol Anti-freezing agent 8.0
11. Naphthol AS pigment
(Permanent Red FGR 112) Pigment 2.0
12. Distilled Water Filler Q.S.

The manufacturing procedure for the composition of Example 4 is similar to the one described in Example 1.

Example 5: Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% (WS)

S. No. Composition Function Charge in (%)
1 Pyraclostrobin (Technical) Active ingredient 2.5
2 Thiophanate-methyl (Technical) Active ingredient 12.0
3 Thiamethoxam (Technical) Active ingredient 25.0
4 Sodium ligno sulphonates Dispersing Agent 5.0
5 Alkyl naphthalene sulphonates formaldehyde condensate Dispersing Agent 2.0
6 Sodium Isopropyl Naphthalene Sulphonate Wetting Agent 3.0
7 Silicon dioxide Co-filler 2.0
8 Vinyl Acetate Copolymer Binder 1.5
9 Naphthol AS pigment
(Permanent Red FGR 03) Pigment 2.0
10 China Clay Filler Q. S.

Procedure of preparation of Pyraclostrobin 2.5% + Thiophanate Methyl 12% + Thiamethoxam 25% WS.

Step 1: According to the batch size, raw materials were mixed in following order.
? Pyraclostrobin technical
? Thiophanate Methyl technical
? Thiamethoxam technical
? Wetting agent
? Dispersing agent
? Co-filler
? Pigment
? Binder
? Filler
Step 2: The sample is pre-mixed in a blender.
Step 3: The sample is milled, through Air Jet mill instrument. Particle Size of milled material is d50 value is <5µm
Step 4: Collecting the Air Jet milled sample and post blending in a blender.
Step 5: Submitting the sample for quality control for complete analysis, and
Step 6: Packing the material in suitable bottle.
Example 6: Pyraclostrobin 1% + Thiophanate methyl 8% + Thiamethoxam 20% (WS)
S. No. Composition Function Charge in (%)
1. Pyraclostrobin (Technical) Active ingredient 1.0
2. Thiophanate-methyl (Technical) Active ingredient 8.0
3. Thiamethoxam (Technical) Active ingredient 20.0
4. Polyvinyl pyrrolidone Binder 2.0
5. Sodium naphthalene sulphonate blend Dispersing agent 5.0
6. Sodium isopropyl naphthalene sulphonate Wetting Agent 5.0
7. Silicon dioxide Co-filler 5.0
8. Naphthol AS pigment
(Permanent Red FGR 03) Pigment 3.0
9. China clay Filler Q.S.

The manufacturing procedure for composition of Example 6 is similar to the one described in Example 5.
Efficacy study of Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% in flowable concentrate for seed treatment (FS):

SYNERGY STUDIES:
A synergistic effect exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components.
After calculating % disease control, the synergism was calculated by the below formula: The synergistic pesticidal action of the inventive mixtures calculated by Colby’s formula is as follows:
Appropriate analysis of plant response to pesticide combination is critical in determining the type of activity observed. The most widely used model is the one Gowing* derived and Colby** modified. Gowing described a mathematical formula for calculating the predicting response values for pesticide mixtures. He suggested the expected (E) percent inhibition of growth induced by pesticide A plus pesticide B is as follows, *(Jerry Flint et al, 1988) ***

The synergistic action expected for a given combination of three active components can be calculated as follows:
(AB + AC + BC) ABC
Expected (E) = A + B + C - +
100 10000
Where:
“E” represents expected percentage of pesticidal control for the combination of the two or three active ingredients at defined doses (for example equal to A, B and C, respectively).
“A” is the percentage of pesticidal control observed by compound A at a defined dose.
“B” is the percentage of pesticidal control observed by the compound B at a defined dose.
“C” is the percentage of pesticidal control observed by the compound C at a defined dose.
The synergistic action expected for given combination of two active components can be calculated as follows:
AB
Expected (E) = A + B -
100
Where:
A - Percentage of pest control observed by compound A.
B - Percentage of pest control observed by compound B.

When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergistic effect. (Ratio of O/E > 1, means synergism observed.)

Reference:
*Gowing, D. P. 1960. Comments on tests of herbicide mixtures. Weeds 8:379–391.
**Colby, S. R. 1967. Calculating synergistic and antagonistic responses of herbicide combinations. Weeds 15:20–22
*** Jerry Flint et al, 1988. Analyzing Herbicide Interactions: A Statistical Treatment of Colby's Method. Weed Technology 2: 304-309

SOYBEAN:
Trial 1: Bio-efficacy against Damping-off and Stem rot:
The soil borne fungi viz., Rhizoctonia, Fusarium and Phytophthora cause severe damage to fresh growing seedlings and serious threat to many field crops at early stage of development and can also affect the crop stand and yield. Rhizoctonia (Root and stem rot) and Sclerotium (Collar rot) are common soybean diseases that typically cause most damage to seedlings but can also damage older plants. It can kill and stunt plants to result in significant yield losses, or the lesions can be superficial and have minimal effects on plant health. For a soil-inhabiting pathogen, many environmental and soil factors are responsible for the development of disease. These soil and seeds born fungi are widely distributed in tropical, subtropical, and temperate regions and attack large number of plants.

The Damping-off, and Stem rot is caused by the fungal pathogen Phythophthora sojae. Phythophthora sojae infected germinating seeds rot before or shortly after emergence. Damping-off and stem rot infected seedling rapidly wilt, turn yellow, and die. Dead leaves characteristically remain attached to the plant. Stems of killed plants exhibit a dark, red-brown stem lesion starting at the soil line and extending up to the second or third node. The root rot phase of phytophthora root and stem rot (PRSR) is not as readily discerned as the killing stem rot phase of the disease. Overall, the root volume of diseased plants is reduced compared with healthy plants. Main and secondary roots appear brown in color and nodule formation is dramatically reduced. The infection spreads most quickly when susceptible varieties are grown under favorable conditions such as after heavy rainfall, the warm temperature (28 to 32°C), high humidity (95% or above), and dense stands with a heavily developed canopy.
The field trial was conducted to evaluate the efficacy of the individual and their binary and ternary mixtures of Pyraclostrobin, Thiophanate-methyl and Thiamethoxam against Damping-off, and Stem rot in Soybean crops. The trial was conducted with randomized block design with net plot size of 5m x 6m. Soybean crops were raised with all standard agronomic practices. The seed rate for Soybean was taken as 75 kg/ha. The “Seed treatment” of product was done before sowing of crop in main field. Seed treatment was done with seed dressing machine by preparing slurry.
The Percent Disease Control (PDC) observation of Damping-off and stem rot were recorded at 15 DAA and 30 DAA (Days after application) as per plot basis. The visual observations were recorded for % disease control per plot basis.
The observations were recorded for % disease control from entire plot against Untreated check. The observations were recorded at 15 DAA and 30 DAA. The data pertaining to the percent Damping-off and Stem rot control is presented in Table 1.
Table 1: Damping-off and stem rot disease control in Soybean:

Compositions Dose
(g A.I./ha) Percent disease control (PDC) – Damping-off and stem rot
15 DAA 30 DAA
Pyraclostrobin 20% WG 18.75 GAH
(0.25 GA/Kg of seed) 60 50
Thiophanate-methyl 70% WP 90 GAH
(1.2 GA/Kg of seed) 70 60
Thiamethoxam 25% WG 187.5 GAH
(2.5 GA/Kg of seed) 20 10
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 18.75 + 90 GAH
(0.25 + 1.2 GA/Kg of seed) 85 75
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 90 + 187.5 GAH
(1.2 + 2.5 GA/Kg of seed) 75 60
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 18.75 + 187.5 GAH
(0.25 + 2.5 GA/Kg of seed) 70 50
Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS 296.25 GAH
(3.95 GA/Kg of seed) 95 90
*GA/Kg – gram active/ Kg of seed

The solo, binary combinations, and ternary combinations of three active ingredients were tested and as per table 1, the ternary combination provides excellent efficacy than the solo and binary combinations.
The trial results show that the ternary combination of Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS composition is very promising and consistent against damping-off and stem rot disease of soybean in terms of efficacy as well as residual control.

Table 2: Damping-off and stem rot disease control in Soybean at 30 DAA:

Compositions Dose
(g A.I./ha) Percent disease control – Damping-off and stem rot
Expected Observed
Pyraclostrobin 20% WG 18.75 GAH
(0.25 GA/Kg of seed) 50
Thiophanate-methyl 70% WP 90 GAH
(1.2 GA/Kg of seed) 60
Thiamethoxam 25% WG 187.5 GAH
(2.5 GA/Kg of seed) 10
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 18.75 + 90 GAH
(0.25 + 1.2 GA/Kg of seed) 80 75
Ratio of O/E 0.93
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 90 + 187.5 GAH
(1.2 + 2.5 GA/Kg of seed) 64 60
Ratio of O/E 0.93
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 18.75 + 187.5 GAH
(0.25 + 2.5 GA/Kg of seed) 55 50
Ratio of O/E 0.90
Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS 296.25 GAH
(3.95 GA/Kg of seed) 82 90
Ratio of O/E 1.097

The results in table 2 clearly demonstrate synergy between Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% FS in controlling damping-off and stem rot disease. As can be seen from table 2, the ternary combination provides better results than the binary compositions. The large difference between the observed and the expected efficacy clearly demonstrates the synergistic effect of the combination.

Trial 2: Bio-efficacy against Stem fly:
The Stem Fly or bean fly (Ophiomyia phaseoli Tryon.) is a major pest insect in different countries across the globe. The extent of damage varies from crop to crop and season to season, being especially severe to seedlings. The adult stem fly deposits their eggs in puncture of the leaf tissues, the first pair of leaves of bean seedlings being favorite sites for oviposition and cause extensive tunnelling to young plants. Overall plant growth is stunted, and it may die; yield losses in some east-Asian countries can come to 30 to 50% and even to 100%.
The field trial was conducted to evaluate the efficacy of the individual and their binary and ternary mixtures of Pyraclostrobin, Thiophanate-methyl and Thiamethoxam against Stem fly in Soybean crop. The trial was conducted with randomized block design with net plot size of 5m x 6m. Soybean crops were raised with all standard agronomic practices. The seed rate for Soybean was taken as 75 kg/ha. The “Seed treatment” of product was done before sowing of crop in main field. Seed treatment was done with seed dressing machine by preparing slurry.
The percent control of Stem fly observation was recorded from the plot size of 5m x 6 m. The observations were recorded at 10 DAA and 20 DAA (Days after application). The visual observations were recorded for % disease control per plot basis.

The percentage of Stem fly control observations were recorded from plot basis against untreated check at an observation interval of 10 DAA and 20 DAA (Days after application). The data pertaining to the percent count of Stem fly presented in Table 3.

Table 3: Bio efficacy of Stem fly in Soybean at 10 and 20 DAA:
Compositions Dose
(g AI/ha) Percent Stem fly control
10 DAA 20 DAA
Pyraclostrobin 20% WG 18.75 GAH
(0.25 GA/Kg of seed) 10 5
Thiophanate- methyl 70% WP 90 GAH
(1.2 GA/Kg of seed) 20 10
Thiamethoxam 25% WG 187.5 GAH
(2.5 GA/Kg of seed) 85 85
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 18.75 + 90 GAH
(0.25 + 1.2 GA/Kg of seed) 15 10
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 90 + 187.5 GAH
(1.2 + 2.5 GA/Kg of seed) 85 82
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 18.75 + 187.5 GAH
(0.25 + 2.5 GA/Kg of seed) 85 82
Pyraclostrobin 2.5% + Thiophanate- methyl 12% + Thiamethoxam 25% FS 296.25 GAH
(3.95 GA/Kg of seed) 92 89
*GA/Kg – gram active/ Kg of seed

The trial results show good efficacy of Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% FS combinations against Stem fly of Soybean at 10 and 20 days after application.
The solo, and binary applications of all three active ingredients of Pyraclostrobin, Thiophanate methyl, and Thiamethoxam were not able to provide satisfactory control of Stem fly disease. The seed treatment combination product of the present invention, Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% (FS) found promising against Stem fly control up to the 20 days after application.

Table 4: Stem fly control in Soybean at 20 DAA
Compositions Dose
(g AI/ha) Percent Stem fly control
Expected Observed
Pyraclostrobin 20% WG 18.75 GAH
(0.25 GA/Kg of seed) 5
Thiophanate- methyl 70% WP 90 GAH
(1.2 GA/Kg of seed) 10
Thiamethoxam 25% WG 187.5 GAH
(2.5 GA/Kg of seed) 85
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 18.75 + 90 GAH
(0.25 + 1.2 GA/Kg of seed) 14.5 10
Ratio of O/E 0.69
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 90 + 187.5 GAH
(1.2 + 2.5 GA/Kg of seed) 86.5 82
Ratio of O/E 0.95
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 18.75 + 187.5 GAH
(0.25 + 2.5 GA/Kg of seed) 85.75 82
Ratio of O/E 0.95
Pyraclostrobin 2.5% + Thiophanate- methyl 12% + Thiamethoxam 25% FS 296.25 GAH
(3.95 GA/Kg of seed) 87.15 89
Ratio of O/E 1.02

The results in table 4 clearly demonstrate synergy between Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS in controlling Stem fly of Soybean. As can be seen from table 4, the ternary combination provides better results than the binary compositions. The large difference between the observed and the expected efficacy clearly demonstrates the synergistic effect of the combination.

GROUND NUT:
Trial 3: Bio-efficacy against Collar rot and White grub in Groundnut:
The soil borne fungi viz., Sclerotium, Aspergillus are important seeds and soil borne diseases of groundnut (Arachis hypogaea L.) which deteriorates kernel quality and reduces yield. It can kill and reduces the plant population per hectare to result in significant yield losses. For a soil-inhabiting pathogen, many environmental and soil factors are responsible for the development of disease. These soil and seeds born fungi are widely distributed in tropical, subtropical, and temperate regions and attack large numbers of plants.
Collar rot caused by Aspergillus niger van Teighem is one of the most important diseases of groundnut which is more extensive in the kharif than the rabi/summer seasons and causes more damage in sandy loam and medium black soil. Annual world yield loss caused by collar rot is more than 10 per cent (Pande and Rao, 2000) and is more prevalent in soils with low moisture content and high temperature, approximately 30ºC (Kishore et al., 2007). The pathogen survives in plant debris in the soil, not necessarily from a groundnut crop. Soil-borne conidia cause disease to carry over from season to season. The other primary source is the infected seeds. The pathogen is also seedborne in nature. The mycelium of the fungus is hyaline to sub-hyaline. Conidiophores arise directly from the substrate and are septate, thick walled, hyaline or olive brown in colour. The vesicles are mostly globes and have two rows of hyaline phialides viz., primary and secondary phialides. The conidial head is dark brown to black. The conidia are globose, dark brown in colour and produced in long chains.
The field trial was conducted to evaluate the efficacy of the individual and their binary and ternary mixtures of Pyraclostrobin, Thiophanate-methyl and Thiamethoxam against collar rot and white grub in groundnut crop. The trial was conducted with randomized block design with net plot size of 5m x 6m. Groundnut crops were raised with all standard agronomic practices. The seed rate for groundnut was taken as 100 kg/ha. The “Seed treatment” of product was done before sowing of crop in main field. Seed treatment was done with seed dressing machine by preparing slurry.
The Percent Disease Control (PDC) observation of Collar rot was recorded at 15 DAA and 30 DAA (Days after application) as per plot basis. The visual observations were recorded for % disease control per plot basis.
The observations were recorded for % disease control from entire plot against Untreated check. The observations were recorded at 15 DAA and 30 DAA. The data pertaining to the percent Collar rot control is presented in Table 5.

Table 5: Collar rot disease control in Groundnut:

Compositions Dose
(g AI/ha) Percent disease control – Collar rot
15 DAA 30 DAA
Pyraclostrobin 20% WG 20 GAH
(0.2 GA/Kg of seed) 65 55
Thiophanate-methyl 70% WP 120 GAH
(1.2 GA/Kg of seed) 75 65
Thiamethoxam 25% WG 250 GAH
(2.5 GA/Kg of seed) 20 10
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 20 +120 GAH
(0.2 + 1.2 GA/Kg of seed) 90 80
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 120 + 250 GAH
(1.2 + 2.5 GA/Kg of seed) 75 60
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 20 + 250 GAH
(0.2 + 2.5 GA/Kg of seed) 85 55
Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS 390 GAH
(3.9 GA/Kg of seed) 95 93
*GA/Kg – gram active/ Kg of seed

The solo, binary combinations, and ternary combinations of three active ingredients were tested and as per table 5, the ternary combination provides excellent efficacy than the solo and binary combinations.
The trial results show that the ternary combination of Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS composition found to be very promising and consistent against collar rot disease of groundnut in terms of efficacy as well as residual control.

Table 6: Collar rot disease control in Groundnut at 30 DAA:

Compositions Dose
(g AI/ha) Percent disease control – Collar rot
Expected Observed
Pyraclostrobin 20% WG 20 GAH
(0.2 GA/Kg of seed) 55
Thiophanate-methyl 70% WP 120 GAH
(1.2 GA/Kg of seed) 65
Thiamethoxam 25% WG 250 GAH
(2.5 GA/Kg of seed) 10
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 20 +120 GAH
(0.2 + 1.2 GA/Kg of seed) 84.25 80
Ratio of O/E 0.94
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 120 + 250 GAH
(1.2 + 2.5 GA/Kg of seed) 68.5 60
Ratio of O/E 0.87
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 20 + 250 GAH
(0.2 + 2.5 GA/Kg of seed) 59.5 55
Ratio of O/E 0.92
Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS 390 GAH
(3.9 GA/Kg of seed) 85.8
93

Ratio of O/E 1.08

The results in table 6 clearly demonstrate synergy between Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% FS in controlling Collar rot disease. As can be seen from table 6, the ternary combination provides better results than the binary compositions. The large difference between the observed and the expected efficacy clearly demonstrates the synergistic effect of the combination.

Trial 4: Bio-efficacy against White grub:
White grubs (Holotrichia serrata) are soil-inhabiting polyphagous pests and are also known as root grubs. They feed on organic matter in soil as well as the root system of many economic crops. They belong to scarabidae family of coleopteran insect order. Holotrichia serrata is most destructive in states like, Karnataka, Andhra Pradesh, Tamil Nadu and Maharashtra and later it has spread to western and peninsular India. White grubs prefer light clay soils rich in organic matter; aeration; moisture and soil temperature around 25ºC. They have a single generation in a year where grub (larvae) is the damaging stage and adults are free living.
The field trial was conducted to evaluate the efficacy of the individual and their binary and ternary mixtures of Pyraclostrobin, Thiophanate-methyl and Thiamethoxam against White grub in groundnut crops. The trial was conducted with randomized block design with net plot size of 5m x 6m. Groundnut crops were raised with all standard agronomic practices. The seed rate for groundnut was taken as 100 kg/ha. The “Seed treatment” of product was done before sowing of crop in main field. Seed treatment was done with seed dressing machine by preparing slurry.
The percent control of White grub observation was recorded from the plot size of 5m x 6m. The observations were recorded at 15 DAA and 30 DAA (Days after application). The visual observations were recorded for % white grub control per plot basis.
The percentage of white grub control observations were recorded from plot basis against untreated check at an observation interval of 15 DAA and 30 DAA (Days after application). The data pertaining to the percentage count of White grubs (Holotrichia serrata) is presented in Table 7.
Table 7: Bio efficacy of White grub in Groundnut at 15 and 30 DAA:
Compositions Dose
(g A.I./ha) Percent White grub Control
15 DAA 30 DAA
Pyraclostrobin 20% WG 20 GAH
(0.2 GA/Kg of seed) 5 5
Thiophanate- methyl 70% WP 120 GAH
(1.2 GA/Kg of seed) 10 5
Thiamethoxam 25% WG 250 GAH
(2.5 GA/Kg of seed) 80 75
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 20 +120 GAH
(0.2 + 1.2 GA/Kg of seed) 15 8
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 120 + 250 GAH
(1.2 + 2.5 GA/Kg of seed) 82 72
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 20 + 250 GAH
(0.2 + 2.5 GA/Kg of seed) 85 70
Pyraclostrobin 2.5% + Thiophanate- methyl 12% + Thiamethoxam 25% FS 390 GAH
(3.9 GA/Kg of seed) 90 80

The trial results show good efficacy of Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% FS combinations against White grubs (Holotrichia serrata) in groundnut at 15 and 30 days after application.
The solo, and binary combinations of all three active ingredients of Pyraclostrobin, Thiophanate methyl, and Thiamethoxam were not able to provide satisfactory control of White grub. The seed treatment combination product of the present invention, Pyraclostrobin 2.5% + Thiophanate methyl 12% + Thiamethoxam 25% (FS) found promising against White grub (Holotrichia serrata) control up to the 30 days after application.

Table 8: White grub control in Groundnut at 30 DAA
Compositions Dose
(g A.I./ha) Percent White grub (Holotrichia serrata) control
Expected Observed
Pyraclostrobin 20% WG 20 GAH
(0.2 GA/Kg of seed) 5
Thiophanate- methyl 70% WP 120 GAH
(1.2 GA/Kg of seed) 5
Thiamethoxam 25% WG 250 GAH
(2.5 GA/Kg of seed) 75
Pyraclostrobin 20% WG +
Thiophanate-methyl 70% WP 20 +120 GAH
(0.2 + 1.2 GA/Kg of seed) 9.75 8
Ratio of O/E 0.82
Thiophanate-methyl 70% WP + Thiamethoxam 25% WG 120 + 250 GAH
(1.2 + 2.5 GA/Kg of seed) 76.25 72
Ratio of O/E 0.94
Pyraclostrobin 20% WG +
Thiamethoxam 25% WG 20 + 250 GAH
(0.2 + 2.5 GA/Kg of seed) 76.25 70
Ratio of O/E 0.91
Pyraclostrobin 2.5% + Thiophanate- methyl 12% + Thiamethoxam 25% FS 390 GAH
(3.9 GA/Kg of seed) 77.43 80
Ratio of O/E 1.03

The results in table 8 clearly demonstrate synergy between Pyraclostrobin 2.5% + Thiophanate-methyl 12% + Thiamethoxam 25% FS in controlling White grub (Holotrichia serrata) of groundnut. As can be seen from table 8, the ternary combination provides better results than the binary compositions. The large difference between the observed and the expected efficacy clearly demonstrates the synergistic effect of the combination.
,CLAIMS:We Claim:

1. A seed treatment composition comprising of a) pyraclostrobin present in a range from 1% (w/w) to 5% (w/w), b) thiophanate-methyl present in a range from 8% (w/w) to 16% (w/w), c) thiamethoxam present in a range from 20% (w/w) to 35% (w/w), and agrochemical additives.

2. The composition as claimed in claim 1, wherein the agrochemical additives are selected from a wetting agent, a dispersing agent, an anti-freezing agent, a biocide, a defoamer, a rheology modifier, a binder, a pigment, a co-filler, a filler and combination thereof.

3. The composition as claimed in claim 2, wherein the wetting agent is selected from the group comprising polyoxyethylene polyoxy propylene condensate, alcohol ethoxylates, sodium alkyl naphthalene sulphonate blend, and sodium isopropyl naphthalene sulfonate, present in a range from 1% to 5% (w/w).

4. The composition as claimed in claim 2, wherein the dispersing agent is selected from the group comprising tristyryl phenyl ether phosphate TEA salt, acrylic co-polymer, tristyryl phenol ethoxylates, sodium ligno sulphonates, alkyl naphthalene sulphonates formaldehyde condensate, sodium naphthalene sulphonate blend, and combination thereof, present in a range from 1% to 10% (w/w).

5. The composition as claimed in claim 2, wherein the anti-freezing agent is propylene glycol, present in a range from 2% to 10% (w/w).

6. The composition as claimed in claim 2, wherein the rheological modifier is xanthan gum present in a range from 1% to 15% (w/w).

7. The composition as claimed in claim 2, wherein the binder is selected from the group comprising vinyl acetate copolymer, vinyl pyrrolidone, and poly vinyl pyrrolidine, present in a range from 0.5% to 3.0% (w/w).

8. The composition as claimed in claim 2, wherein the pigment is selected from the group comprising naphthol AS-based pigments, [(4E)-N-(2-methylphenyl)-3-oxo-4-[(2,4,5-trichlorophenyl)hydrazinylidene] naphthalene-2-carboxamide3-hydroxy-n-(2-methylphenyl)-4-((2,4,5-trichlorophenyl) azo)naphthalene-2-carboxamide)], and [(4E)-N-(2-methylphenyl)-3-oxo-4-[(2,4,5-trichlorophenyl) hydrazinylidene] naphthalene-2-carboxamide 3-hydroxy-n-(2-methylphenyl)-4-((2,4,5-trichlorophenyl)azo) naphthalene-2-carboxamide)], present in a range from 0.5% to 4.0% (w/w).

9. The composition as claimed in claim 2, wherein the biocide is 1, 2-Benzisothiazol-3(2H)-one, present in a range from 0.05% to 0.5% (w/w).

10. The composition as claimed in claim 2, wherein the defoamer is selected from silicon powder, and silicone emulsion, present in a range from 0.5% to 2.0% (w/w).

11. The composition as claimed in claim 2, wherein the co-filler is silicon dioxide, present in a range from 2% to 5% (w/w) and the filler is selected from distilled water, and china clay, present in Q.S.

12. The composition as claimed in claim 1, wherein the composition is in form of flowable concentrate for seed treatment (FS) and water dispersible powder for slurry seed treatment (WS).