Description:FIELD OF THE INVENTION

[0001] The present invention relates to a medicinal plants-based hair color and care powder composition and method for preparation thereof that is made from natural ingredients to provide protection against premature greying by shielding hair follicles from UV radiation in view of restoring hair’s natural shine and promoting growth.

BACKGROUND OF THE INVENTION

[0002] There is an increasing demand for a medicinal plants-based hair color and care products due to the rising awareness of skin sensitivities and allergies caused by common ingredients in traditional hair care products. Many commercially available powders, shampoos, and conditioners contain fragrances, preservatives, and chemicals that can irritate the scalp or trigger allergic reactions in sensitive individuals. This has led to a growing need for hair care solutions that are gentle, hypoallergenic, and safe for all skin types. The natural powders offer an alternative by using natural, non-irritating ingredients, reducing the risk of allergies while effectively cleaning, conditioning, and nourishing the hair. Such compositions cater to individuals with sensitive skin or allergies, offering a safer and more comfortable hair care experience.

[0003] Currently, there are several alternatives to traditional hair care products, such as fragrance-free or hypoallergenic powders. These often use milder, plants-based ingredients or compositions specifically developed for sensitive skin. However, many of these alternatives still contain preservatives, chemicals, or fillers that cause irritation or allergic reactions in some individuals. Additionally, some natural-based hair care powders may lack sufficient cleansing or conditioning power, leaving hair feeling dry or unclean. Moreover, fragrance-free products may have a less pleasant sensory experience, and some hypoallergenic options might not provide the same level of effectiveness in managing specific hair concerns like dandruff or oil control. However, these products may still contain preservatives, fragrances, or surfactants that irritate sensitive skin, especially for those with specific allergies to certain plants extracts or synthetic chemicals.

[0004] Additionally, many natural hair care powders available today, such as dry shampoos made with cornstarch or rice powder absorb excess oil. While these options are typically free from harsh chemicals, they may leave a powdery residue on the hair and scalp, which could be difficult to remove or may not provide adequate cleaning. Furthermore, some natural products might not offer enough conditioning or moisturizing properties, leading to dryness or fizziness in hair.

[0005] US8586105B2 discloses a topical hair care formulation and method of making includes combining herbal powders to form a herbal powder mixture. The herbal powder mixture is steeped in an amount of carrier oil for a period of time to form an infused oil. A portion of an amount of olive oil is combined with an amount of biotin and an amount of an organosulfur compound to form a natural mixture. A remainder of the amount of olive oil is combined with the infused oil. An amount of jojoba oil is combined with the infused oil. An amount of rosemary oil is combined with the infused oil. An amount of lavender oil is combined with the infused oil. The natural mixture is combined with the infused oil. The infused oil is settled for a period of time to form the topical hair care formulation. Although US’105 discloses a topical hair care formulation and method of making includes combining herbal powders to form a herbal powder mixture. However, the cited art lacks in providing a hair care solution for delaying the onset of grey hair through protection against UV radiation.

[0006] WO2014174116A2 discloses a composition for dyeing keratin fibres, comprising i) at least % and preferably at least 5% of red henna (Lawsonia inermis, alba) powder and/or of powder of indigo-producing plant(s), ii) at least one oil, iii) optionally at least one surfactant, and iv) water or an aqueous phase, preferably water, it being understood that the ingredients 10 ii), iii) and iv) are predispersed together to form a predispersion and the ingredient(s) i) are then mixed into the said predispersion or the said predispersion is mixed with the ingredient(s) i); the invention also relates to a composition comprising the said composition and a supplement of water or of aqueousphase, the process for dyeing keratin fibres using the said compositions, and to the use of the said compositions for dyeing keratin fibres.1 The compositions and the poultice according to the invention make it possible to dye keratin fibres with strong, chromatic dyeing results that are resistant to washing, perspiration, sebum and light, and that are moreover long-lasting, without impairing the said fibres. Furthermore, the use of the compositions or of the poultice does not give off any raw material 20 dust (dust-free). These compositions are easy to use, in total safety and with no risk of staining. In addition, the composition and the dyeing active agent(s) remain stable on storage. The treated keratin fibres have a very pleasant cosmetic aspect, their integrity is respected. Although WO’116 discloses a composition for dyeing keratin fibres. However, the cited art lacks in providing a hair care solution that is capable of stimulating melanocyte activity to increase melanin production.

[0007] Conventionally, many herbal powder compositions are available in the market. However, the available compositions do not contain blend of different herbal ingredients that provide a complete hair care solution by sstimulating melanocyte activity to increase melanin production, while also delaying the onset of grey hair through protection against UV radiation.

[0008] In order to overcome the aforementioned drawbacks, there exists a need in the art to develop a medicinal plants-based hair color and care powder composition that provide a natural solution made from a blend of herbal extracts to provide an effective, nourishing and zero allergy alternative to traditional hair dyes by using plants-based ingredients to color hair while promoting its health and strength, thus providing optimal care to the hair.

OBJECTS OF THE INVENTION

[0009] The principal object of the present invention is to overcome the disadvantages of the prior art.

[0010] An object of the present invention is to develop a medicinal plants-based hair color and care powder composition that consists of natural ingredients in view of restoring the natural shine of hair, providing a smoother and shinier finish.

[0011] Another object of the present invention is to develop a medicinal plants-based hair color and care powder composition that provides a blended herbal powder to be effectively incorporated into hair colorants and hair care masks/ubtans in view of promoting natural hair growth and preventing hair loss.

[0012] Another object of the present invention is to develop a medicinal plants-based hair color and care powder composition that exhibits protective properties against premature greying by providing a protective barrier to shield the hair from ultraviolet (UV) radiation that damage hair follicles and reduce melanin production, leading to premature greying.

[0013] Yet another object of the present invention is to develop a medicinal plants-based hair color and care powder composition that have the ability to delay or prevent the onset of grey hair by stimulating melanocyte activity within the hair follicles, thus encouraging the production of melanin.

[0014] The foregoing and other objects, features, and advantages of the present invention will become readily apparent upon further review of the following detailed description of the preferred embodiment as illustrated in the accompanying drawings.

SUMMARY OF THE INVENTION

[0015] The present invention relates to a medicinal plants-based hair color and care powder composition and method for preparation thereof that incorporates all natural ingredients to encourage hair growth by stimulating melanocyte activity to increase melanin production, and delaying the onset of grey hair.

[0016] According to an embodiment of the present invention, a medicinal plants-based hair color and care powder composition, comprises of: i) Lawsonia Inermis (Henna) leaf extract powder in the range of 30-40% v/v, ii) Cassia Angustifolia (Senna) leaf extract powder in the range of 25-35% v/v, iii) Withania Somnifera (Ashwagandha) root extract powder in the range of 3-8% v/v, iv) Aloe Barbadensis (Aloe Vera) leaf extract powder in the range of 8-12% v/v, v) Rosmarinus Officinalis (Rosemary) leaf extract powder in the range of 3-8% v/v, vi) Azadirachta Indica (Neem) leaf extract powder in the range of 3-8% v/v, vii) Tribulus Terrestris (Gokshura) seed extract powder in the range of 2-6% v/v, and viii) Alizarin (Ratanjot) root extract powder in the range of 3-8% v/v.

[0017] According to another embodiment of the present invention, a method for preparation of the a medicinal plants-based hair color and care powder composition comprises of the following steps: a) taking Cassia Obovata (Colorless Henna) leaves and demineralized water in a ratio of 1:5 in a round bottom flask and attaching the flask to a heating mantle; b) attaching Clevenger apparatus with water tubing, metal rotating clamp to the support stand rod and collection flask, followed by adjusting temperature of distillation to 80-110oC in order to obtain steam distilled Cassia Obovata leaf extract, c) adding dry Lawsonia Inermis (Henna) leaves for maceration process for a time duration of 70-75 hours to obtain a solution, followed by adding the solution to a round bottom flask, d) setting up steam distillation and Clevenger apparatus and adjusting temperature to 80-110oC in order to obtain steam distilled Lawsonia Inermis leaf extract and Lawsonia Inermis leaf precipitate, followed by drying the precipitate through incubation at a temperature of 100-110oC and grinding into fine powder to obtain Lawsonia Inermis leaf extract powder, e) subjecting Cassia Angustifolia (Senna) leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Cassia Angustifolia (Senna) leaf extract powder and subjecting the powder to grinding to obtain fine powder, followed by blending the Lawsonia Inermis leaf extract powder and Cassia Angustifolia (Senna) leaf extract powder for 5-15 minute minutes in order to obtain powder blend 1, f) subjecting Withania Somnifera root extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Withania Somnifera root extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 1 powder and Withania Somnifera root extract powder for 5-15 minute minutes in order to obtain powder blend 2, g) subjecting Aloe Barbadensis leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Aloe Barbadensis leaf extract powder and subjecting the powder to grinding to obtain fine powder, followed by mixing the powder blend 2 and the Aloe Barbadensis leaf extract powder for 5-15 minute minutes in order to obtain powder blend 3, h) subjecting Rosmarinus Officinalis leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Rosmarinus Officinalis leaf extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 3 powder and Rosmarinus Officinalis leaf extract powder for 5-15 minute minutes in order to obtain powder blend 4, i) subjecting Azadirachta Indica leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Azadirachta Indica leaf extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 4 powder and the Azadirachta Indica leaf extract powder for 5-15 minute minutes in order to obtain powder blend 5, j) subjecting Tribulus Terrestris seed extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Tribulus Terrestris seed extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 5 powder and the Tribulus Terrestris seed extract powder for 5-15 minute minutes in order to obtain powder blend 6, and k) subjecting Alizarin root extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Alizarin root extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 6 powder and the Alizarin root extract powder for 5-15 minute minutes in order to obtain powder blend 7 i.e. a medicinal plants-based hair color and care powder composition.

[0018] While the invention has been described and shown with particular reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

[0019] These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:
Figure 1 illustrates a flow chart depicting a method for preparation of a medicinal plants-based hair color and care powder composition;
Figure 2 illustrates another flow chart of the proposed method in continuation with the figure 1;
Figure 3 illustrates another flow chart of the proposed method in continuation with the figure 2;
Figure 4 illustrates a graphical representation of cell proliferation rate relative to the control group; and
Figure 5 illustrates a graphical representation of RT PCR analysis of cyclin D1 expression in DP Cells after 12 hrs. of incubation with the indicated concentration of a medicinal plants-based hair color and care powder composition.

DETAILED DESCRIPTION OF THE INVENTION

[0020] The following description includes the preferred best mode of one embodiment of the present invention. It will be clear from this description of the invention that the invention is not limited to these illustrated embodiments but that the invention also includes a variety of modifications and embodiments thereto. Therefore, the present description should be seen as illustrative and not limiting. While the invention is susceptible to various modifications and alternative constructions, it should be understood, that there is no intention to limit the invention to the specific form disclosed, but, on the contrary, the invention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the invention as defined in the claims.

[0021] In any embodiment described herein, the open-ended terms "comprising," "comprises,” and the like (which are synonymous with "including," "having” and "characterized by") may be replaced by the respective partially closed phrases "consisting essentially of," consists essentially of," and the like or the respective closed phrases "consisting of," "consists of, the like.

[0022] As used herein, the singular forms “a,” “an,” and “the” designate both the singular and the plural, unless expressly stated to designate the singular only.

[0023] The present invention relates to a medicinal plants-based hair color and care powder composition and method for preparation thereof consisting of natural herbs to promote hair growth by stimulating melanocyte activity to increase melanin production, as well as delaying the onset of grey hair by creating a barrier against UV radiation, thus providing a complete haircare solution with no side-effects.

[0024] According to an embodiment of the present invention a medicinal plants-based hair color and care powder composition, comprises of: i) Lawsonia Inermis (Henna) leaf extract powder in the range of 30-40% v/v, ii) Cassia Angustifolia (Senna) leaf extract powder in the range of 25-35% v/v, iii) Withania Somnifera (Ashwagandha) root extract powder in the range of 3-8% v/v, iv) Aloe Barbadensis (Aloe Vera) leaf extract powder in the range of 8-12% v/v, v) Rosmarinus Officinalis (Rosemary) leaf extract powder in the range of 3-8% v/v, vi) Azadirachta Indica (Neem) leaf extract powder in the range of 3-8% v/v, vii) Tribulus Terrestris (Gokshura) seed extract powder in the range of 2-6% v/v, and viii) Alizarin (Ratanjot) root extract powder in the range of 3-8% v/v.

[0025] The preferable value of i) Lawsonia Inermis (Henna) leaf extract powder is 35% v/v, ii) Cassia Angustifolia (Senna) leaf extract powder is 30% v/v, iii) Withania Somnifera (Ashwagandha) root extract powder is 6% v/v, iv) Aloe Barbadensis (Aloe Vera) leaf extract powder is 10% v/v, v) Rosmarinus Officinalis (Rosemary) leaf extract powder is 5% v/v, vi) Azadirachta Indica (Neem) leaf extract powder is 5% v/v, vii) Tribulus Terrestris (Gokshura) seed extract powder is 4% v/v, and viii) Alizarin (Ratanjot) root extract powder is 5% v/v.

[0026] The herbs used herein (except Lawsonia Inermis (Henna) leaf extract) are subjected to hydro-distillation with the help of Clevenger apparatus to obtain precipitate.

[0027] The precipitates are prepared by using plants part (leaves/stem/flower/root) and demineralized water in a ratio of 1:5.

[0028] The extraction is carried out at a temperature in the range of 80-110oC for 7-8 hours.

[0029] According to another embodiment of the present invention, a method for preparation of the medicinal plants-based hair color and care powder composition comprises of the following steps: a) taking Cassia Obovata (Colorless Henna) leaves and demineralized water in a ratio of 1:5 in a round bottom flask and attaching the flask to a heating mantle; b) attaching Clevenger apparatus with water tubing, metal rotating clamp to the support stand rod and collection flask, followed by adjusting temperature of distillation to 80-110oC in order to obtain steam distilled Cassia Obovata leaf extract, c) adding dry Lawsonia Inermis (Henna) leaves for maceration process for a time duration of 70-75 hours to obtain a solution, followed by adding the solution to a round bottom flask, d) setting up steam distillation and Clevenger apparatus and adjusting temperature to 80-110oC in order to obtain steam distilled Lawsonia Inermis leaf extract and Lawsonia Inermis leaf precipitate, followed by drying the precipitate through incubation at a temperature of 100-110oC and grinding into fine powder to obtain Lawsonia Inermis leaf extract powder, e) subjecting Cassia Angustifolia (Senna) leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Cassia Angustifolia (Senna) leaf extract powder and subjecting the powder to grinding to obtain fine powder, followed by blending the Lawsonia Inermis leaf extract powder and Cassia Angustifolia (Senna) leaf extract powder for 5-15 minute minutes in order to obtain powder blend 1, f) subjecting Withania Somnifera root extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Withania Somnifera root extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 1 powder and Withania Somnifera root extract powder for 5-15 minute minutes in order to obtain powder blend 2, g) subjecting Aloe Barbadensis leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Aloe Barbadensis leaf extract powder and subjecting the powder to grinding to obtain fine powder, followed by mixing the powder blend 2 and the Aloe Barbadensis leaf extract powder for 5-15 minute minutes in order to obtain powder blend 3, h) subjecting Rosmarinus Officinalis leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Rosmarinus Officinalis leaf extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 3 powder and Rosmarinus Officinalis leaf extract powder for 5-15 minute minutes in order to obtain powder blend 4, i) subjecting Azadirachta Indica leaf extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Azadirachta Indica leaf extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 4 powder and the Azadirachta Indica leaf extract powder for 5-15 minute minutes in order to obtain powder blend 5, j) subjecting Tribulus Terrestris seed extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Tribulus Terrestris seed extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 5 powder and the Tribulus Terrestris seed extract powder for 5-15 minute minutes in order to obtain powder blend 6, and k) subjecting Alizarin root extract precipitate to incubation for dying at 100-110oC temperature to obtain in order to obtain Alizarin root extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 6 powder and the Alizarin root extract powder for 5-15 minute minutes in order to obtain powder blend 7 i.e. the medicinal plants-based hair color and care powder composition.

[0030] The powders of the extracts used herein are mixed with the help of a Cone Blender Machine.

[0031] The powders used herein are sieved through a mesh of size 100.

EXAMPLE

[0032] Referring to Figure 1, a flow chart depicting a method for preparation of a medicinal plants-based hair color and care powder composition is illustrated. The method for preparation of the medicinal plants-based hair color and care powder composition comprises of the following steps: firstly, collection of herbal plants residue after extracting liquid extract, followed by drying herbs extract recommended temperature in a hot air oven is between 40–60°C for 4-5 Hours.

[0033] Then, the powdering was achieved by any suitable means, such as grinding or milling machine. The grinding machine was operated at slow rpm with a big mesh size to produce herbal material of a relatively larger size, whereas a high speed and small mesh size to form the powder. Particles larger than the mesh remain in the machine and may be subjected to further be grinding. The particle size of powdered herbal extract was minimum 95% passed through a mesh of 100 mesh size.

[0034] To start the preparation, Cassia Obovata (Colorless Henna) leaves and demineralized water was taken in a ratio of 1:5 in a round bottom flask and attaching the flask to a heating mantle, followed by attaching Clevenger apparatus with water tubing, metal rotating clamp to the support stand rod and collection flask, and adjusting temperature of distillation to 80-110oC in order to obtain steam distilled Cassia Obovata leaf extract.

[0035] Then, adding dry Lawsonia Inermis (Henna) leaves for maceration process for a time duration of 70-75 hours to obtain a solution, followed by adding the solution to a round bottom flask, and setting up steam distillation and Clevenger apparatus at 80-110oC in order to obtain steam distilled Lawsonia Inermis leaf extract and Lawsonia Inermis leaf precipitate. The obtained precipitate was dried through incubation at a temperature of 100-110oC and grinding into fine powder to obtain Lawsonia Inermis leaf extract powder,

[0036] Similarly, Cassia Angustifolia (Senna) leaf extract precipitate was subjected to incubation for dying at 105oC temperature to obtain in order to obtain Cassia Angustifolia (Senna) leaf extract powder and grinded to obtain fine powder, followed by blending the Lawsonia Inermis leaf extract powder and Cassia Angustifolia (Senna) leaf extract powder for 10 minute minutes in order to obtain powder blend 1.

[0037] After that, Withania Somnifera root extract precipitate was incubated for dying at 105oC temperature to obtain Withania Somnifera root extract powder, and subjecting the powder to grinding, followed by mixing the powder blend 1 powder and Withania Somnifera root extract powder for 10 minute minutes in order to obtain powder blend 2.

[0038] Then, Aloe Barbadensis leaf extract powder was prepared by incubating Aloe Barbadensis leaf extract precipitate for dying at 105oC temperature and grinding to obtain fine powder. The Aloe Barbadensis leaf extract powder was mixed with powder blend 2 for 10 minute minutes in order to obtain powder blend 3.

[0039] The powder blend 3 was mixed with Rosmarinus Officinalis leaf extract powder, obtained in the same manner as other precipitates were incubated for dying at 105oC, and powdered by grinding. The mix of powder blend 3 and Rosmarinus Officinalis leaf extract powder obtained powder blend 4. Then, the powder blend 4 powder was mixed with Azadirachta Indica leaf extract powder for 5-15 minute minutes in order to obtain powder blend 5.

[0040] The powder blend 5 was mixed with Tribulus Terrestris seed extract powder for 10 minute minutes in order to obtain powder blend 6.

[0041] At last step, Alizarin root extract precipitate was dried to obtain Alizarin root extract powder, and grinding the powder to obtain optimum sized particles. The obtained powder blend 6 was then mixed with Alizarin root extract powder for 10 minute minutes in order to obtain powder blend 7 i.e. the medicinal plants-based hair color and care powder composition.

CHARACTERIZATION

[0042] The efficacy of the prepared medicinal plants-based hair color and care powder composition was tested through Primary Irritation Patch Test, Melanin content or Anti-greying effect, and Cell Proliferation Assay Using Cyclin D1 Expression Marker in Dermal Papilla.

Primary Irritation Patch Test

[0043] For conducting the patch test, 24 healthy adult humans were selected as subjects. The evaluation of the product to be tested was carried out versus a positive control: 1% (w/w) SLS, and versus a negative control: 0.9% isotonic solution. Out of 24 subjects screened, none were found screen failures, none were dropouts. 24 subjects underwent the Trial. The mean age of the subjects was 36.4 years. The mean height of the subjects was 161cm. The mean weights of the subjects were 67 kg. Data from all 24 subjects who completed the study were analyzed. There were no subjects lost to follow up. There was no subject who withdrawn the consent. Result: The medicinal plants-based hair color and care powder composition was assessed by qualified dermatologists and was found to be non-irritating and showed zero allergy during the 24-hour Primary Irritation Patch Test. Starting on day 1st, followed by 2nd day. A follow up visit was done after 1 week of patch removal, and the composition was still found non-irritating and showed zero allergy.

Melanin content or Anti-greying effect

[0044] The study was done to observe the effects of the medicinal plants-based hair color and care powder composition on melanin production in melanocyte cells, specifically using the WM 266-4 cell line. Melanocytes are specialized cells located in the hair follicles that produce melanin, the pigment responsible for hair color. Over time, melanocyte activity decreases, leading to the greying of hair. The WM 266-4 melanocyte cell line was treated with varying concentrations of the medicinal plants-based hair color and care powder composition to assess its impact on melanin production. The control group consisted of untreated melanocytes, while the experimental groups were treated with the test article. The cells were incubated for a defined period, and the melanin content was then measured using standard biochemical techniques.

[0045] Result: The results showed a significant increase in melanin content in the melanocytes treated with the medicinal plants-based hair color and care powder composition compared to the control group. Specifically, the melanin content in the treated cells was increased by 44.95% relative to the untreated control sample. This substantial increase suggests that the medicinal plants-based hair color and care powder composition actively promotes the synthesis of melanin in melanocytes, potentially enhancing pigmentation within the hair follicles.

Cell Proliferation Assay Using Cyclin D1 Expression Marker in Dermal Papilla

[0046] This study focused on evaluating the effects of the medicinal plants-based hair color and care powder composition on the proliferation of dermal papilla cells (DPCs). Dermal papilla cells are located in the hair follicles that help in regulating hair growth and the overall health of the hair follicle. The study assesses the impact of the medicinal plants-based hair color and care powder composition on this process, comparing its effects to those of a control group that received no treatment. The study involved treating dermal papilla cells (DPCs) with different concentrations of the medicinal plants-based hair color and care powder composition, specifically at 5% and 10% concentrations, while a control group was used for comparison. Proliferation rates were quantified by cell count and other methods to measure cellular activity.

[0047] In addition to measuring cell proliferation, the study also focused on DNA synthesis, which occurs in the S phase of the cell cycle. DNA synthesis is a key indicator of cell division and proliferation. To assess this, cyclin D1 expression was measured in the treated and control groups using reverse transcription polymerase chain reaction (RT-PCR), a molecular biology technique that quantifies the expression of specific genes. Cyclin D1 is a regulatory protein that plays a critical role in the progression of the cell cycle, particularly during the transition from the G1 to the S phase, where DNA synthesis occurs.

[0048] Result: Referring to Figure 4, a graphical representation of cell proliferation rate relative to the control group is illustrated. The results revealed that the medicinal plants-based hair color and care powder composition significantly enhanced the proliferation of dermal papilla cells compared to the vehicle control group. Referring to Figure 5, a graphical representation of RT PCR analysis of cyclin D1 expression in DP Cells after 12 hrs is illustrated. Specifically, at a 5% concentration of the test article, cell proliferation increased by 35.2%, and at a 10% concentration, the increase was even more significant, with a 97.8% rise in cell proliferation compared to the control group.

[0049] These results suggest that the herbal composition has a potent stimulatory effect on dermal papilla cell activity, potentially promoting hair follicle regeneration and hair growth. Furthermore, the study found that the medicinal plants-based hair color and care powder composition promoted DNA synthesis during the S phase of the cell cycle that indicated enhanced cell division and DNA replication, crucial for the maintenance and regeneration of hair follicles.

[0050] Although the field of the invention has been described herein with limited reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the invention, will become apparent to persons skilled in the art upon reference to the description of the invention. , Claims:1) A medicinal plants-based hair color and care powder composition, comprising:

i) Lawsonia Inermis (Henna) leaf extract powder in the range of 30-40% v/v;

ii) Cassia Angustifolia (Senna) leaf extract powder in the range of 25-35% v/v;

iii) Withania Somnifera (Ashwagandha) root extract powder in the range of 3-8% v/v;

iv) Aloe Barbadensis (Aloe Vera) leaf extract powder in the range of 8-12% v/v;

v) Rosmarinus Officinalis (Rosemary) leaf extract powder in the range of 3-8% v/v;

vi) Azadirachta Indica (Neem) leaf extract powder in the range of 3-8% v/v;

vii) Tribulus Terrestris (Gokshura) seed extract powder in the range of 2-6% v/v; and

viii) Alizarin (Ratanjot) root extract powder in the range of 3-8% v/v.

2) The composition as claimed in claim 1, wherein said herbs (except Lawsonia Inermis (Henna) leaf extract) are subjected to hydro-distillation with the help of Clevenger apparatus to obtain precipitate.

3) The composition as claimed in claim 1, wherein each of said precipitates are prepared by using plants part (leaves/stem/flower/root) and demineralized water in a ratio of 1:5.

4) The composition as claimed in claim 1, wherein extraction is carried out at a temperature in the range of 80-110oC for 7-8 hours.

5) A method for preparation of said a medicinal plants-based hair color and care powder composition as claimed in claim 1 and 3, is incubation of said precipitates for dying said at 100-110oC temperature.