Description:PLEASE SEE THE ATTACHMENTS. , Claims:1. A cartridge (100) for analysis of a biological fluid, the cartridge (100) comprising:
a base plate (1) defining a flow surface,
the base plate (1) defining an inlet (2) for introducing the biological fluid,
wherein the flow surface of the base plate (1) includes:
10 a first passage (3) fluidly connected to the inlet (2), configured to receive
the biological fluid from the inlet (2), wherein the first passage (3) fluidly
connects to a first analysis chamber (4); and
a second passage (5) extending from the first passage (3), the second
passage (5) configured to receive the biological fluid from the first passage (3)
15 and in fluid connection with a reagent chamber (7), wherein the reagent chamber
(7) comprises a plurality of reagents to lyse, react and stain the biological fluid
in a predetermined time; and
wherein the second passage (5) is configured to extend from the reagent
chamber (7) and fluidly connect to a second analysis chamber (6) on the base
20 plate (1), to receive stained biological fluid from the reagent chamber (7).
2. The cartridge (100) as claimed in claim 1, comprises a feed boot (2’) defined around
circumference of the inlet (2) configured to guide the biological fluid into the inlet (2).
25 3. The cartridge (100) as claimed in claim 1, the second passage (5) comprises a serpentine
passage (8) defined between the reagent chamber (7) and the second analysis chamber
(6) configured to mix the plurality of reagents from the reagent chamber (7) during flow
of the biological fluid.
30 4. The cartridge (100) as claimed in claim 3, wherein diameter of the serpentine passage
(8) is less than diameter of the second passage (5) for restricted flow of the biological
fluid.
5. The cartridge (100) as claimed in claim 3, comprises a third analysis chamber (9)
35 connected between the serpentine passage (8) and the second analysis chamber (6) to
delay the flow of the biological fluid to the second analysis chamber (6).
6. The cartridge (100) as claimed in claim 5, wherein depth of the first analysis chamber
(4) is in a range of 10 to 50 microns, depth of the second analysis chamber (6) is 3 to 5
21
times to the depth of the first analysis chamber (5 4) and depth of the third
analysis chamber (9) is 5 to 12 times to the depth of the second analysis chamber (6).
7. The cartridge (100) as claimed in claim 5, wherein the first analysis chamber (4) allows
imaging of the biological fluid by a first microscopic analysis, the second analysis
10 chamber (6) allows imaging of the biological fluid by a second microscopic analysis
and wherein the third analysis chamber (9) allows to analyze the biological fluid by a
third microscopic analysis.
8. The cartridge (100) as claimed in claim 5, wherein the biological fluid is configured to
15 flow from one end to another end of each of the first analysis chamber (4), the second
analysis chamber (6) and the third analysis chamber (9).
9. The cartridge (100) as claimed in claim 8, wherein flow of the biological fluid in the
first analysis chamber (4), the second analysis chamber (6) and the third analysis
20 chamber (9) is balanced by capillary force and an atmospheric pressure.
10. The cartridge (100) as claimed in claim 9, wherein the base plate (1) is coated with a
surface modification reagent to allow the biological fluid to flow on the flow surface
by the capillary force.
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11. The cartridge (100) as claimed in claim 1, wherein depth of the reagent chamber (7) is
in a range of 0.3 to 3 mm.
12. The cartridge (100) as claimed in claim 1, wherein the plurality of reagents in the
30 reagent chamber (7) are in a semi-solid or gel form with viscosity in a range of 6
centipoise to 910 centipoise.