Description:Begin with shrimp shell waste. Wash, dry, and grind the shells to obtain shrimp shell powder. Demineralization treats the shrimp shell powder with distilled water and HCl solution to remove minerals such as magnesium, sodium and calcium content. Deproteination involves the mixing with alkaline solutions (1.3 N, 4% HCl, 14 mL/g, 24 hours) to remove proteins and lipids. Deacetylations further treat the obtained chitin (from deproteinization) with alkaline hydrolysis subject to a sodium hydroxide (1.25 N, 55% NaOH, 12 mL/g, 24 hours) to convert it into chitosan powder.
For the detection of chitosan, 2-3 drops of potassium iodide solution are added to the chitosan dissolved solution. Potassium iodide is commonly used as a reagent to test for the presence of polysaccharides due to its characteristic reaction with glycosidic linkages. If chitosan is present in the solution, a precipitate forms turning dark brown. Following the addition of Potassium iodide, the solution is acidified with 3-6 drops of sulphuric acid. Then the dark brown precipitate undergoes another colour change, turning dark purple. Also the pH sensor is used to find the presence of the chitosan in its dissolved state, whose value is around 7.4.
For the preparation of thin film from the chitosan powder, we use maceration to extract substances from the pumpkin leaves, resulting in a pumpkin leaves extract. Film Formation combines the pumpkin leaves extract with chitosan (a biopolymer) and glycerol (a plasticizer). The mixture is then used for biodegradable film forming.

, Claims:1. Extraction of chitosan from shrimp shells is used to detect the presence of chitosan in shrimp shells and to prepare the thin film.
2. Extraction of chitosan from shrimp shells is used to detect the presence of chitosan in shrimp shells and to prepare the thin film of claim 1, wherein said this paper attempts to explain the process, and assess its impact.
3. Extraction of chitosan from shrimp shells is used to detect the presence of chitosan in shrimp shells and to prepare the thin film of claim 1, wherein said this paper has many applications.
4. Extraction of chitosan from shrimp shells is used to detect the presence of chitosan in shrimp shells and to prepare the thin film of claim 1, wherein said that this paper discusses the major advantages and how it can improve.