Description:FORM 2

THE PATENTS ACT 1970
(SECTION 39 OF 1970)

&

THE PATENT RULES, 2003

COMPLETE SPECIFICATION
(Section 10 and Rule 13)

PESTICIDAL COMPOSITION OF TRICYCLAZOLE, AZOXYSTROBIN, AND PYMETROZINE

We, COROMANDEL INTERNATIONAL LIMITED,
an Indian company incorporated under Companies Act of 1956,
having its registered office at Coromandel House, Sardar Patel Road,
Secunderabad – 500 003, Telangana, India.

The following specification particularly describes the invention, and the manner in which it is to be performed.

FIELD OF THE INVENTION

The present invention relates to novel synergistic pesticidal composition of fungicides and an insecticide. Particularly, the present invention relates to a pesticidal composition of tricyclazole, azoxystrobin, and pymetrozine along with suitable agrochemical additives in synergistic effective amounts for the broad-spectrum control of pests.

BACKGROUND OF THE INVENTION

Pesticides are substances which are used to control pests (Insects, Diseases, or weeds). The Food and Agriculture Organization (FAO) has defined pesticide as: any substance or mixture of substances intended for preventing, curating, controlling or destroying any pest, including vectors of human or animal disease, unwanted species of plants or animals, causing harm during or otherwise interfering with the production, processing, storage, transport, or marketing of food, agricultural commodities, wood and wood products or animal feedstuffs, or substances that may be administered to animals for the control of insects, arachnids, or other pests in or on their bodies. The term includes substances intended for use as a plant growth regulator, defoliant, desiccant, or agent for thinning fruit or preventing the premature fall of fruit. Also used as substances applied to crops either before or after harvest to protect the commodity from deterioration during storage and transport.

It is known in the art to use several fungicides and insecticides individually or in binary combinations. However, as resistance develops over time, greater dosages are required, which has a detrimental impact on plant health and yield. Therefore, a combination is required that not only lessens the likelihood of resistance development but also enhances the spectrum of disease, pest management, crop yield, and health.

Tricyclazole is a protective triazole fungicide and disclosed in US4064261. It is chemically known as 5-methyl-1,2,4-triazolo (3,4-b) benzothiazole.

Tricyclazole is highly resistant to erosion and does not need to be refilled in the event of rain after one hour of spraying. Mainly to inhibit spore germination and attachment spore formation, thereby effectively preventing the invasion of pathogens and reducing the production of bacterial spores.

Azoxystrobin is a systemic, broad-spectrum fungicide, chemically known as Methyl (2E)-2-(2-{[6-(2-cyanophenoxy) pyrimidin-4-yl]oxy}phenyl)-3-methoxyprop-2-enoate.

Azoxystrobin possesses a broad spectrum of activity, in common with other QoI inhibitors. Examples of the fungal groups on which it is effective are Ascomycota, Deuteromycota, and Basidiomycota, as well as the oomycetes.

Pymetrozine was first disclosed in US4931439 and US4996325. Pymetrozine is chemically known as (E)-4,5-dihydro-6-methyl-4-(3-pyridylmethyleneamino)-1,2,4-triazin-3(2H)-one.

The mode of action of pymetrozine in insects has not been precisely determined biochemically, but it may involve effects on nutriregulation or nerve-muscle interaction. Physiologically, it appears to act by preventing these insects from inserting their stylus into the plant tissue.

IN315396 GSP CROP SCIENCE describes an agrochemical composition comprising of bioactive amount of Pymetrozine along with Triazole, wherein Triazole may be selected from Propiconazole, Tebuconazole, Hexaconazole, Flusilazole or Tricyclazole.
IN358361 GSP CROP SCIENCE describes a synergistic pesticidal composition of bioactive amounts of A) Pymetrozine B) an insecticide and C) a fungicide wherein at least one insecticide is selected from Clothianidin, Flonicamid, Flupyradifurone, Imidacloprid, Nitenpyram, Sulfoxaflor, Thiamethoxam, Tolfenpyrad and Triflumezopyrim and at least one fungicide is selected from Cyproconazole, Difenoconazole, Epoxiconazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole.

IN202111057805 SEEDLING IND describes a synergistic pesticidal composition for the control of plant pests. More particularly, the present invention relates to novel synergistic pesticidal composition comprising (A) Pymetrozine or its agrochemically acceptable salts; (B) Isoprothiolane or its agrochemically acceptable salts; (C) at least one strobilurin fungicide selected from Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Trifloxystrobin, Kresoxim-methyl and Picoxystrobin or its agrochemically acceptable salts; (D) one or more agrochemically acceptable excipients.

However still there is a need for a composition of specific fungicides and insecticides combinations, which overcomes some of the existing problems and can be prepared easily without much complex manufacturing process.

The object of the present invention is to provide a novel and effective synergistic pesticidal composition which has both preventive and curative action and is active against all life stages of major plant pathogens.

OBJECTIVE OF THE INVENTION

One object of the present invention is to provide a novel and effective synergistic pesticidal composition demonstrating high efficacy and high selectivity.

In an object of the present invention is to provide an improved combinations of fungicides and insecticide for the broad-spectrum control of pests.

Another object of the present invention is to provide a novel synergistic pesticidal composition comprising of tricyclazole, azoxystrobin, pymetrozine, and suitable agrochemical additives.

Another object of the present invention is to provide improved combinations of fungicides and insecticide for the broad-spectrum control of pests.

Another object of the present invention is to provide a method and a synergistic composition for controlling insect pests and fungal diseases.

SUMMARY OF THE INVENTION

The present invention discloses a novel synergistical pesticidal composition comprising of fungicide and an insecticide, wherein the fungicides are tricyclazole, and azoxystrobin, and an insecticide is pymetrozine, along with suitable agrochemical additives in synergistically effective amounts.

According to the present invention, a pesticidal composition comprising of tricyclazole is present in a range from 10% (w/w) to 35% (w/w); azoxystrobin is present in a range from 5% (w/w) to 20% (w/w); pymetrozine is present in a range from 1% (w/w) to 15% (w/w), and suitable agrochemical additives in synergistically effective amounts.

According to the present invention, the suitable agrochemical additives are selected from the group comprising a wetting agent, a dispersing agent, a disintegration agent, a defoamer, an anticaking agent, and a filler.

According to the present invention, the wetting agent is selected from the group comprising of sodium alkyl naphthalene sulfonate blend, sodium isopropyl naphthalene sulfonate, naphthalene sulfonic acid and phenol sulphonic acid sodium salt, iso decyl alcohol ethoxylates, and sodium alkyl (butyl and dibutyl) naphthalene sulfonate, present in a range from 1% to 3% (w/w).

According to the present invention, the dispersing agent is selected from the group comprising of alkyl naphthalene sulfonate formaldehyde condensate, sodium ligno sulphonates, kraft lignin polymer, mixture of naphthalene sulfonic acid and phenol sulphonic acid condensate sodium salt, modified styrene acrylic polymer, modified sodium ligno sulphonates, and combination thereof, present in a range from 1% to 10% (w/w).

According to the present invention, the disintegration agent is selected from ammonium sulphate, sodium sulphate (anhydrous), and urea formaldehyde copolymer, present in a range from 10% to 25% (w/w).

According to the present invention, the defoamer is silicon emulsion, present in a range from 0.1% to 2% (w/w).

According to the present invention, an anti-caking agent is silicon dioxide, present in a range from 0.1% to 5% (w/w).

According to the present invention, the filler is china clay, present in Q.S.

In an aspect of the present invention, the composition is formulated as water dispersible granule (WDG).

In an aspect of the present invention provides a method of protecting a plant propagation material, a plant, parts of a plant and/or plant organs that grow at a later point in time against pathogenic damage or pest damage by applying present composition to the plant propagation material.

In an aspect of the present invention, the pesticidal composition controls various pests in field crops, vegetables, oil seeds, and pulses, horticulture & forestry.

In another aspect of the present invention, the pesticidal composition can be applied as a foliar spray to cotton, wheat, soyabean, pulses, and vegetables including but not limited to okra, tomato, sugar beet, egg-plants, lettuce, iceberg lettuce, pepper, cucumber, squash, melon, bean, dry-beans, peas, leek, garlic, onion, cabbage, carrot, tuber such as potato, sugar cane, tobacco, coffee, turf and forage, cruciferous, cucurbits, grapevines, pepper, fodder beet, oil seed rape, pansy, impatiens, petunia and geranium, etc.

The present invention also relates to the process for preparing the synergistic pesticidal composition comprising of Tricyclazole, Azoxystrobin, and Pymetrozine, along with suitable agrochemical additives.

DESCRIPTION OF THE INVENTION

The term "comprising", which is synonymous with "including", "containing", or "characterized by” here is defined as being inclusive or open-ended, and does not exclude additional, unrecited elements or method steps, unless the context clearly requires otherwise.

It is to be noted that, as used in the specification, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
As used herein, the term “synergistic composition” may be understood to include effective combination of more than one agrochemical that allows the application of the said agrochemical in a much lower dosage, which results in a less dosage treatment for the crops.

As used herein, the term “agrochemical additives” may be understood to include a range of surfactants, dispersing agents, pigments, solvents, co-solvents, defoamers, and emulantioxidants, lighton inhibitors, viscosity modifiers, suspending agents, dyes, antioxidants, light absorbers, mixing aids, neutralizers or pH adjusting substances and buffers, corrosion inhibitors, fragrances, wetting agents, absorption enhancers, micronutrients, plasticizers, lubricants, thickeners, anti-freezing agents, sterilization agents, biocide, adjuvant among others.
In an embodiment of the present invention provides a novel synergistical pesticidal composition comprising of fungicides, and an insecticide, wherein the fungicides are Tricyclazole, and Azoxystrobin, and an insecticide is Pymetrozine, along with suitable agrochemical additives in synergistically effective amounts.

The pesticidal composition of the present invention may be formulated as Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) Tablet (TB), Water dispersible granules (WG or WDG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC), a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW) among others. . Preferably, the composition of the present invention is in form of water dispersible granules (WDG).

In an embodiment of the present invention provides a novel synergistical pesticidal composition comprising of fungicides, and an insecticide, wherein the fungicides are tricyclazole, and azoxystrobin, and an insecticide is pymetrozine, along with suitable agrochemical additives in synergistically effective amounts.

The present invention provides a synergistic pesticidal composition comprising of tricyclazole is present in a range from 10% (w/w) to 35% (w/w); azoxystrobin is present in a range from 5% (w/w) to 20% (w/w); pymetrozine is present in a range from 1% (w/w) to 15% (w/w), and suitable agrochemical additives.

According to the present embodiment, the suitable agrochemical additives are selected from the group comprising a wetting agent, a dispersing agent, a disintegration agent, a defoamer, an anticaking agent, and a filler.

A wetting agent is a substance that when added to a liquid increases the spreading or penetration power of the liquid by reducing the interfacial tension between the liquid and the surface on which it is spreading. Wetting agents are used for two main functions in agrochemical compositions: during processing and manufacture to increase the rate of wetting of powders in water to make concentrates for soluble liquids or suspension concentrates; and during mixing of a product with water in a spray tank or other vessel to reduce the wetting time of wettable powders and to improve the penetration of water into water-dispersible granules.

Accordingly, the composition of the present invention contains a wetting agent selected from the group comprising of sodium alkyl naphthalene sulfonate blend, sodium isopropyl naphthalene sulfonate, naphthalene sulfonic acid and phenol sulphonic acid sodium salt, iso decyl alcohol ethoxylates, and sodium alkyl (butyl and dibutyl) naphthalene sulfonate, present in a range from 1% to 3% (w/w).

A dispersant or a dispersing agent is a substance which absorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersants are added to agrochemical compositions to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into water in a spray tank. They are widely used in wettable powders; suspension concentrates and water-dispersible granules.

Accordingly, the composition of the present invention contains a dispersing agent is selected from the group comprising of alkyl naphthalene sulfonate formaldehyde condensate, sodium ligno sulphonates, kraft lignin polymer, mixture of naphthalene sulfonic acid and phenol sulphonic acid condensate sodium salt, modified styrene acrylic polymer, modified sodium ligno sulphonates, and combination thereof, present in a range from 1% to 10% (w/w).

The alkyl naphthalene sulfonates used in the compositions preferably have alkyl groups with 1 to 10 carbon atoms, such as methyl, isopropyl, n-butyl, sec-butyl, and nonyl. Especially preferred products are sodium butyl naphthalene sulfonate and sodium nonyl naphthalene sulfonate.

Disintegrating agents are additive that can be some time useful for improving dispersibility of herbicide formulation. Accordingly, the composition of the present invention contains a disintegration agent selected from ammonium sulphate, sodium sulphate (anhydrous), and urea formaldehyde copolymer, present in a range from 10% to 25% (w/w).

Defoamer is a chemical reagent developed for defoaming and foam suppression based on the principle of foaming. The defoamer suitable for the purpose of the present invention is silicon emulsion, present in a range from 0.1% to 2% (w/w).

An anti-caking agent is an additive used in the pesticide industry to avoid the formation of lumps. The anti-caking agent suitable for the purpose of the present invention is silicon dioxide, present in a range from 0.1% to 5% (w/w).

A filler refers to solid chemicals that are added to an herbicide formulation to aid in the delivery of the active ingredient. Accordingly, the composition of the present invention contains a filler is selected from china clay, starch, and Aluminium hydroxide, present in Q.S.

In an embodiment of the present invention, the composition is formulated as water dispersible granule (WDG).

The process for preparing the present agrochemical composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the composition. However, all such variations and modifications are still covered by the scope of present invention.

Examples:

Example 1: Tricyclazole 22.5% + Azoxystrobin 12% + Pymetrozine 7.5% WDG

S. No. Composition Function Charge in (%)
1. Tricyclazole technical Active ingredient 22.5
2. Azoxystrobin technical Active ingredient 12.0
3. Pymetrozine technical Active ingredient 7.5
4. Sodium alkyl naphthalene Sulfonate blend Wetting agent 2.0
5. Alkyl naphthalene sulfonate formaldehyde condensate Dispersing agent 4.0
6. Ammonium sulphate Disintegrating Agent 15.0
7. Silicone emulsion Defoamer 0.5
8. Silicon dioxide Anti caking agent 3.0
9. China clay Filler Q.S.

The process for manufacture of composition is as follows: 22.5 gm of Tricyclazole, 12.0 gm of Azoxystrobin, 5.5 gm of Pymetrozine, 2.0 gm of sodium alkyl naphthalene sulfonate blend, 4.0 gm of alkyl naphthalene sulfonate formaldehyde condensate, 15.0 gm of ammonium sulphate, 0.5 gm of silicon emulsion, 3.0 gm of silicon dioxide, and required quantity of china clay were weighed in the pre-blender reactor and mixed for 1 hour. The mixture was milled through air jet mill instrument (Inlet pressure 2-3 kg/cm2, grinding pressure 6 kg/ cm2) to obtain the desired particle size (D90 < 12 microns). Collecting the milled material and post blending the material for 1 hour. Extruding the granule at 35 rpm (1.0 mesh), collecting the water dispersible granules, and drying at 50 °C for 30 min (moisture content it should be < 5%) followed by packing the material in a suitable package.
Example 2: Tricyclazole + Azoxystrobin + Pymetrozine WDG
S. No. Composition Function (% w/w) Range
1. Tricyclazole technical Active ingredient 10-35%
2. Azoxystrobin technical Active ingredient 5-20%
3. Pymetrozine technical Active ingredient 1-15%
4. Sodium alkyl naphthalene sulfonate blend, sodium isopropyl naphthalene sulfonate, naphthalene sulfonic acid and phenol sulphonic acid sodium salt, iso decyl alcohol ethoxylates, and sodium alkyl (butyl and dibutyl) naphthalene sulfonate. Wetting agent 1-3%
5. Alkyl naphthalene sulfonate formaldehyde condensate, sodium ligno sulphonates, kraft lignin polymer, mixture of naphthalene sulfonic acid and phenol sulphonic acid condensate sodium salt, modified styrene acrylic polymer, modified sodium ligno sulphonates, and combination thereof. Dispersing agent 1-10%
6. Ammonium sulphate, sodium sulphate (anhydrous), and urea formaldehyde copolymer. Disintegrating Agent 10-25%
7. Silicone emulsion Defoamer 0.1-2%
8. Silicon dioxide Anti caking agent 0.1-5%
9. China clay Filler Q.S.

The manufacturing process is given below:
Step-1: According to the batch size, all raw materials were mixed in the following order.
• Tricyclazole Technical
• Azoxystrobin Technical
• Pymetrozine Technical
• Wetting agent
• Dispersing agent
• Anticaking agent
• Disintegrating agent
• Defoamer
• Filler
Step 2: Pre-mix the sample in a blender.
Step 3: Milling the sample, through Air Jet mill instrument.
Step 4: Collecting the Air Jet milled sample and post blend in a blender.
Step 5: Preparing a mixture of water and defoamer for dough preparation.
Step 6: Extruding the granules through Basket extruder, the temperature of extruder does not go beyond 40 0C.
Step 7: Collecting the WDG and dry in fluid bed dryer (FBD) @ 60°C, check the moisture content it should be < 5.0%.
Note: While drying the WDG in FBD, air flow rate should be maintained between 50-70.
Step 8: Submitting the sample for Analysis.
Step 9: Packing the material.

Evaluation of synergistic effect of the pesticidal composition of the present invention:

A synergistic effect exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components.

After calculating % disease control, the synergism was calculated by below formula:

The synergistic pesticidal action of the inventive mixtures calculated by Colby’s formula as follows:

Appropriate analysis of plant response to fungicide combination is critical in determining the type of activity observed. The most widely used model is the one Gowing* derived and Colby** modified. Gowing described a mathematical formula for calculating the predicting response values for pesticide mixtures. He suggested the expected (E) percent inhibition of growth induced by pesticide A plus pesticide B is as follows, *(Jerry Flint et al, 1988) ***

The synergistic action expected for a given combination of three active components can be calculated as follows:
(AB + AC + BC) ABC
Expected (E) = A + B + C - +
100 10000

Where:
“E” represents expected percentage of pesticidal control for the combination of the two or three active ingredients at defined doses (for example equal to A, B and C, respectively).
“A” is the percentage of pesticidal control observed by the compound (Tricyclazole) at a defined dose.
“B” is the percentage of pesticidal control observed by the compound (Azoxystrobin) at a defined dose.
“C” is the percentage of pesticidal control observed by the compound (Pymetrozine) at a defined dose.

The synergistic action expected for given combination of two active components can be calculated as follows:

AB
Expected (E) = A + B –
100
Where:
A - Percentage of pest control observed by compound A.
B - Percentage of pest control observed by compound B.

When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergistic effect. (Ratio of O/E > 1, means synergism observed.)

Reference:
*Gowing, D. P. 1960. Comments on tests of herbicide mixtures. Weeds 8:379–391.
**Colby, S. R. 1967. Calculating synergistic and antagonistic responses of herbicide combinations. Weeds 15:20–22
*** Jerry Flint et al, 1988. Analyzing Herbicide Interactions: A Statistical Treatment of Colby's Method. Weed Technology 2: 304-309

Field Bio-efficacy trials: Rice Sheath blight, Brown planthopper (BPH), & Rice blast.

Rice Sheath blight:

The sheath blight is caused by the fungal pathogen Rhizoctonia solani. This is potentially devasting disease can infect rice foliage at any stage of crop development. Its major threat to many crops and can also affect the crop stand and yield Rhizoctonia solani fungus is cosmopolitan, polyphagous, widely distributed in tropical, subtropical, and temperate regions and for its development required hot and humid environment. The fungus spreads in the field by growing its runner hyphae from tiller to tiller, from leaf to leaf, and from plant to plant, resulting in a circular pattern of damage. The infection spreads most quickly when susceptible varieties are grown under favorable conditions such as warm temperature (28 to 32°C), high humidity (95% or above), and dense stands with a heavily developed canopy.

Brown planthopper (BPH):

Two species of planthopper infest rice. These are the brown planthopper (BPH), Nilaparvata lugens (Stal); and the white backed planthopper (WBPH), Sogatella furcifera (Horvath). High population of planthoppers cause leaves to initially turn orange yellow before becoming brown and dry and this is a condition called hopper burn that kills the plant. BPH can also transmit Rice Ragged Stunt and Rice Grassy Stunt diseases. Neither disease can be cured. Planthoppers can be a problem in rainfed and in irrigated wetland environments. It also occurs in areas with continuous submerged conditions in the field, high shade, and humidity. Closed canopy of the rice plants, densely seeded crops, excessive use of nitrogen, and early season insecticide spraying also favors insect development.

Hopper burn is like the feeding damage or "bug burn" caused by the rice black bug. To confirm hopper burn caused by planthoppers, check for the presence of sooty molds at the base of the plant. The feeding damage caused by planthoppers results in the yellowing of the plants. At high population density, hopper burns, or complete drying of the plants is observed. At this level, crop loss may be 100%.

Rice blast disease:
The rice blast is caused by the fungal pathogen Magnaporthe oryzae. This is potentially devasting disease can infect rice foliage. The symptoms of rice blast include lesions that can be found on all parts of the plant, including leaves, leaf collars, necks, panicles, pedicels, and seed. It’s a major threat to rice crops and can also affect the crop stand and yield. Infection of rice occurs when conidia are deposited on rice tissues and germinate by producing a germ tube and an appressorium. The appressorium is a melanized structure, and from it develops an infection peg which penetrates the tissue. After penetration, the primary infection hypha grows rapidly and ramifies within susceptible tissues. Growth within tissues of resistant cultivars is often inhibited. Generally, rice blast is favored by moderate temperatures (24 oC) and periods of high moisture that are 12 hours or longer, conditions readily attainable in flooded rice fields. Spores produced as the primary inoculum on the overwintering tissues produce the initial infections on young seedlings when the spores that are deposited on leaves, germinate, and invade leaf tissues.

The field trial was conducted to evaluate the efficacy of innovative mixtures of Tricyclazole, Azoxystrobin, Pymetrozine against rice sheath blight, brown plant hopper (BPH), and rice blast diseases in rice crop. The trial was conducted with randomized block design with net plot size of 5m x 6m. rice crop was raised with all standard agronomic practices. Spraying was done with manual operated backpack knapsack sprayer with 500 L of water spray volume per hectare at 45 days after transplanting.

The visual observations were recorded for % disease control from ten hills per plot. The observations were recorded before spraying, 7 DAA (Days after application) and 14 DAA (Days after application).

Table 1: Control of Rice Sheath blight, Brown planthopper (BPH), & Rice blast on rice crop:
Compositions Dose
(g AI/ha) % disease control –
rice sheath blight % disease control – BPH % disease control – rice Blast
07 DAA 14 DAA 07 DAA 14 DAA 07 DAA 14 DAA
Tricyclazole 75% WP 225 70 75 10 5 70 75
Pymetrozine 50 % WG 120 0 0 85 90 0 0
Azoxystrobin 23% SC 75 65 70 0 0 50 55
Tricyclazole 75% WP + Pymetrozine 50% WG 225 + 120 70 70 82 85 65 70
Tricyclazole 75% WP + Azoxystrobin 23% SC 225 + 75 80 90 0 0 75 80
Pymetrozine 50 % WG + Azoxystrobin 23% SC 120 + 75 65 65 82 85 45 50
Tricyclazole 22.5% + Azoxystrobin 12% + Pymetrozine 7.5 WDG 225+75+
120 100 100 88 92 100 100

The innovative mixture of Tricyclazole 22.5% + Azoxystrobin 12% + Pymetrozine 7.5% WDG provides an excellent control of rice sheath blight, brown plant hopper (BPH), and rice blast diseases with long residual control.

Table 2: Synergistic activity against rice sheath blight, brown plant hopper (BPH), & rice blast disease.
Compositions Dose
(g AI/ha) % disease control – rice sheath blight % disease control- BPH control % disease control –
rice blast
Expected Observed Expected Observed Expected Observed
Tricyclazole 75% WP 225 75 5 75
Pymetrozine 50 % WG 120 0 90 0
Azoxystrobin 23% SC 75 70 0 55
Tricyclazole 75% WP + Pymetrozine 50% WG 225 + 120 75 70 90.8 88 75 70
Ratio of O/E 0.93
0.96 0.93
Tricyclazole 75% WP + Azoxystrobin 23% SC 225 + 75 92.5 90 0 0 88.75 85
Ratio of O/E 0.97
0.00 0.95
Pymetrozine 50 % WG + Azoxystrobin 23% SC 120 + 75 70 65 90 85 55 50
Ratio of O/E 0.92
0.94 0.90
Tricyclazole 22.5% + Azoxystrobin 12% + Pymetrozine 7.5 WDG 225+75+
120 92.5 100 90.5 92 88.75 98
Ratio of O/E 1.08
1.02 1.10

It can be seen that the synergism was observed between Tricyclazole 22.5% + Azoxystrobin 12% + Pymetrozine 7.5 WDG in terms of excellent controlling the rice sheath blight, brown plant hopper (BPH), and rice blast diseases in rice crop.
, Claims:We Claim:

1. A synergistic pesticidal composition comprising tricyclazole, azoxystrobin, pymetrozine and agrochemical additives.

2. The composition as claimed in claim 1, wherein in the composition tricyclazole is present in a range from 10% (w/w) to 35% (w/w); azoxystrobin is present in a range from 5% (w/w) to 20% (w/w); pymetrozine is present in a range from 1% (w/w) to 15% (w/w), and agrochemical additives.

3. The composition as claimed in claim 1, wherein the composition is formulated as water dispersible granules (WDG) form.

4. The composition as claimed in claim 1, wherein the agrochemical additives are selected from a wetting agent, a dispersing agent, a disintegration agent, a defoamer, an anticaking agent, and a filler.

5. The composition as claimed in claim 4, wherein the wetting agent is selected from the group comprising of sodium alkyl naphthalene sulfonate blend, sodium isopropyl naphthalene sulfonate, naphthalene sulfonic acid and phenol sulphonic acid sodium salt, iso decyl alcohol ethoxylates, and sodium alkyl (butyl and dibutyl) naphthalene sulfonate, present in a range from 1% to 3% (w/w).

6. The composition as claimed in claim 4, wherein the dispersing agent is selected from the group comprising of alkyl naphthalene sulfonate formaldehyde condensate, sodium ligno sulphonates, kraft lignin polymer, mixture of naphthalene sulfonic acid and phenol sulphonic acid condensate sodium salt, modified styrene acrylic polymer, modified sodium ligno sulphonates, and combination thereof, present in a range from 1% to 10% (w/w).

7. The composition as claimed in claim 4, wherein the disintegration agent is selected from ammonium sulphate, sodium sulphate (anhydrous), and urea formaldehyde copolymer, present in a range from 10% to 25% (w/w).

8. The composition as claimed in claim 4, wherein the defoamer is silicon emulsion, present in a range from 0.1% to 2% (w/w).

9. The composition as claimed in claim 4, wherein the anti-caking agent is silicon dioxide, present in a range from 0.1% to 5% (w/w).

10. The composition as claimed in claim 4, wherein the filler is china clay, present in Q.S.

Dated this fifteenth (15th) day of May 2024

Sahadev Katam,
General Manager, Legal-IPR,
Coromandel International Limited,
Registered Indian Patent Agent (IN/PA/5560).