DESC:FORM 2

THE PATENTS ACT, 1970
(SECTION 39 OF 1970)
&
The Patents Rules, 2003

COMPLETE SPECIFICATION
(Section 10 and Rule 13)

SYNERGISTIC PESTICIDAL NEONICOTINOID COMBINATION FORMULATIONs, PROCESS FOR PREPARING AND USE THEREOF

We, COROMANDEL INTERNATIONAL LIMITED,
an Indian company incorporated under Companies Act of 1956, having its principal place of business at Coromandel House, Sardar Patel Road, Secunderabad – 500 003, Telangana, India.

The following specification particularly describes the invention and the manner in which it is to be performed:
FIELD OF THE INVENTION

The present invention relates to synergistic pesticidal composition comprising combination of a neonicotinoid and an aromatic ether derivative.

The present invention also relates to synergistic pesticidal composition comprising combination of a neonicotinoid, an aromatic ether derivative and other agrochemical auxiliaries.

The present invention specifically relates to emulsifiable concentrate (EC) composition comprising combination of Nitenpyram and Pyriproxyfen.

The present invention specifically relates to emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen at a weight ratio of 1 : 5 to 5 : 1 of first active ingredient to the second active ingredient.

The present invention specifically relates to emulsifiable concentrate composition comprising combination of Nitenpyram, Pyriproxyfen, emulsifier and other agrochemical auxiliaries.

The present invention also relates to a process for the preparation of emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen, wherein the composition is prepared by simple blending and milling process.

BACKGROUND OF THE INVENTION
Nitenpyram is a synthetic, nicotine-related insecticide, has an effect on the nicotinic acetylcholine receptors. This compound is an insect neurotoxin belonging to the class of neonicotinoids which works by blocking neural signalling of the central nervous system. It does so by binding irreversibly to the nicotinic acetylcholine receptor (nACHr) causing a stop of the flow of ions in the postsynaptic membrane of neurons leading to paralysis and death.

Nitenpyram is chemically known as (E)-N-(6-chloro-3-pyridylmethyl)-N-ethyl-N'-methyl-2-nitrovinylidenediamine and has the following structure:

Pyriproxyfen is a pesticide which is found to be effective against a variety of insects. It was introduced to the US in 1996, to protect cotton crops against whitefly. It has also been found useful for protecting other crops. It is also used as prevention for flea control on household pets, for killing indoor and outdoor ants and roaches. Methods of application include aerosols, bait, carpet powders, foggers, shampoos and pet collars. Pyriproxyfen is a juvenile hormone analog and an insect growth regulator. It prevents larvae from developing into adulthood and thus rendering them unable to reproduce. Pyriproxyfen affects many types of insects, including fleas, cockroaches, ticks, ants, carpet beetles and mosquitoes.

Pyriproxyfen is chemically known as 4-phenoxyphenyl(R/S)-2-(2-pyridyloxy)propylether 2-[1-(4-phenoxyphenoxy)propan-2-loxy]pyridine and has the following structure:

Nitenpyram has been used on many commercial crops, such as cotton and corn, and can be applied in various ways. Commonly used techniques are dusting and seed treatment. Seed treatment allows for a long lasting immunity to insects damaging the crops. The use of Nitenpyram has been shown to be highly effective in protecting crops, as it's generally less toxic for non-target organisms, while killing off crop-destroying insects.

Nitenpyram is used for controlling of Aphids, Thrips, Leafhoppers, Whitefly, and other sucking insects on rice and glasshouse crops. Nitenpyram applied on rice, applied at 15-75 g/ha (foliar), 75-100 g/ha (dust) or 300-400 g/ha (soil treatment) and also for control of fleas on cats and dogs.

Though, conventionally each of these active ingredients are used individually, combination product is not approved which has advantages compared to the mono product. Hence, there is increased interest in the use of active ingredients in combination to give synergistic results.

The given below prior-art references show the use of combinations of Nitenpyram and Pyriproxyfen generically in different ratios.

CN101796957A generically claims combination of Nitenpyram and Pyriproxyfen in the weight ratio of 40: 1~1: 40. The use of carrier, auxiliary agent and the functional additive etc. of adaptation for use, as thinner, antifreezing agent, thickener, stabilizing agent and defoamer etc. The combinations can be made into different formulation products according to instructions for use, as emulsifiable concentrates, wetting powder, microemulsion, water emulsions, water suspensions, water dispersible granules etc.

CN101971810A generically claims combination of Nitenpyram and Pyriproxyfen. The combination composition disclosed in this application contains one or more auxiliary agents such as emulsifier, wetting agent, dispersant, defoamer, stabilizing agent, adhesive etc. and processes. Preparation 4 discloses microemulsion composition which contains water. However, the specific compositions of Nitenpyram and Pyriproxyfen of the present invention are not disclosed in these documents.

Accordingly, there is a need in the art to provide pesticidal synergistic compositions which are environmentally safer and stable pesticidal compositions having two active ingredients with different physical and chemical properties with enhanced efficacy and stable product during storage.

It was surprisingly and unexpectedly found by the present inventors that a selective combination composition of two active ingredients comprising Nitenpyram, Pyriproxyfen and other agrochemical auxiliaries, possesses enhanced efficacy, improved penetration and long duration control of phytopathogens as compared to its other traditional formulations with long storage stability across a wide temperature range and having no adverse impact on photosynthetic activity in plant and seed quality.

OBJECTIVE OF INVENTION
The main objective of the present invention is to provide synergistic pesticidal composition comprising combination of neonicotinoid and an aromatic ether derivative.

Another objective of the present invention is to provide synergistic pesticidal composition comprising combination of a neonicotinoid, an aromatic ether derivative and other agrochemical auxiliaries.

Another objective of the present invention is to provide emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen.

Another objective of the present invention is to provide synergistic emulsifiable concentrate composition comprising combination of Nitenpyram, Pyriproxyfen and other agrochemical auxiliaries.
Another objective of the present invention is to provide emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen at a weight ratio of 1 : 5 to 5 : 1 of first active ingredient to the second active ingredient.

Another objective of the present invention is to provide emulsifiable concentrate composition comprising combination of Nitenpyram, Pyriproxyfen, emulsifier and other agrochemical auxiliaries.

Another objective of the present invention is to provide a process for the preparation of emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen, wherein said composition is prepared by simple blending and homogenization process.

SUMMARY OF INVENTION
Accordingly, the present invention provides synergistic pesticidal composition comprising combination of neonicotinoid and an aromatic ether derivative.

In one embodiment, the present invention provides synergistic pesticidal composition comprising combination of a neonicotinoid, an aromatic ether derivative and other agrochemical auxiliaries.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprising combination of Nitenpyram, Pyriproxyfen and other agrochemical auxiliaries.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen at a weight ratio of 1 : 5 to 5 : 1 of first active ingredient to the second active ingredient.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen at a weight of 1 : 5 to 5 :1 of first active ingredient to the second active ingredient, emulsifiers and other agrochemical auxiliaries.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprises:
1% to 30% (w/w) of Nitenpyram as first active ingredient,
1% to 40% (w/w) of Pyriproxyfen as second active ingredient,
1% to 30% (w/w) of emulsifiers, and
0.1% to 70% (w/w) of other additives / auxiliaries.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprises:
1% to 30% (w/w) of Nitenpyram,
1% to 40% (w/w) of Pyriproxyfen,
1% to 30% (w/w) of emulsifiers,
1% to 70% (w/w) of solvents, and optionally
10% to 20% (w/w) of inert carrier or fillers.

In another embodiment, the present invention provides an emulsifiable concentrate composition comprising:
1% to 30% (w/w) of Nitenpyram,
1% to 40% (w/w) of Pyriproxyfen,
1% to 10% (w/w) of alkoxylated butyl ether EO-PO,
1% to 20% (w/w) of tristyryl phenol ethoxylate,
1% to 30% (w/w) of dimethyl sulfoxide, and
1% to 40% (w/w) of diethylene glycol dimethyl ether.
In another embodiment, the present invention provides a process for preparing emulsifiable concentrate composition comprising the steps of:
a. weighing all the active ingredients,
b. mixing all the ingredients in homogenizer,
c. filtering the obtained material and packing.

In another embodiment, the present invention provides a process for preparing emulsifiable concentrate composition comprising the steps of:
a. weighing and mixing Nitenpyram, Pyriproxyfen, alkoxylated butyl ether EO-PO, tristyryl phenol ethoxylate, dimethyl sulfoxide and diethylene glycol dimethyl ether.
b. filtering the obtained material and packing.

DETAILED DESCRIPTION OF THE INVENTION
The term "comprising", which is synonymous with "including", "containing", or "characterized by" here is defined as being inclusive or open-ended, and does not exclude additional, unrecited elements or method steps, unless the context clearly requires otherwise.

The present invention provides synergistic composition comprising combination of 1 : 5 to 5 : 1 of first active ingredient to the second active ingredient, more preferably in the ratio of 1 : 3 to 3 : 1. However, variables of the ratios of each of the active ingredients can be used and the selection of the particular amount is dependent upon many factors including, for example, type of formulations, the crop, disease sought to be controlled and environmental conditions. The selection of the proper quantity of active agent to be applied, however, is within the expertise of one skilled in the art.

The composition of the present invention could be applied to plants, seeds, fruits, soil as preventive and curative state.
ECs are versatile formulations that can be applied with many types of sprayers. EC compositions enable spontaneous emulsification upon dilution and are easy to handle and measure. Very little agitation is required to mix them with water and will not separate; little visible residue on the plant; do not wear sprayer parts or plug screens or nozzles; seldom leaves visible residue

The emulsifier used in the composition allows the active ingredient in the solvent to mix with water, these forms an emulsion. The emulsifying surfactant system enabling the EC to form an oil-in-water emulsion when the formulation is added to water is typically a mixture of two or more surfactants, at least one of which is a nonionic surfactant.

The surfactants customarily employed in formulation technology, which are described, inter alia, in “Mc Cutcheon's detergents and emulsifiers annual” MC Publishing Corp., Ridgewood N.J., 1981, Stache, H., “Tensid-Taschenbuch”, Carl Hanser Verlag, MunichNienna, 1981 and M. and J. Ash, “Encyclopedia of Surfactants”, Vol I-III, Chemical Publishing Co., New York, 1980-81, are also suitable for preparation of the herbicidal compositions according to the invention.

Anionic surfactants suitable for use in the invention may be any known in the art. The anionic surfactants may be polyarylphenol polyalkoxyether sulfates and/or phosphates; C8-18 alcohol polyalkoxyether phosphates, carboxylates, and/or citrates; alkyl benzenesulfonic acids; C8-20 alkyl carboxylates including fatty acids; C8-20 alcohol sulfates; C8-20 alcohol phosphate mono- and diesters; C8-20 alcohol and (C8-20alkyl)phenol polyoxyethylene ether carboxylates, sulfates and sulfonates; C8-20 alcohol and (C8-20 alkyl)phenol polyoxyethylene phosphate mono- and diesters; C8-20 alkylbenzene sulfonates, naphthalene sulfonates and formaldehyde condensates thereof; lignosulfonates; C8-20 alkyl sulfosuccinates and sulfosuccinamates; C8-20 acyl glutamates, sarcosinates, isethionates and taurates; water-soluble soaps and mixtures thereof.
Exemplary polyarylphenol polyalkoxyether sulfates and phosphates include polyarylphenol polyethoxyether sulfates and phosphates, polyarylphenol polypropoxyether sulfates and phosphates, polyarylphenol poly(ethoxy/propoxy)ether sulfates and phosphates, and salts thereof. The term “aryl” includes, for example, phenyl, tolyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl, styryl, pyridyl, quinolinyl, and mixtures thereof. Exemplary polyarylphenol polyethoxyether sulfates and phosphates include distyrylphenol polyethoxyether sulfates and phosphates, and tristyrylphenol polyethoxyether sulfates and phosphates. The polyarylphenol polyalkoxether sulfates and phosphates may have a degree of alkoxylation (e.g., ethoxylation) of between about 1 and about 50, preferably between about 2 and about 40, more preferably between about 5 and about 30. Commercially available polyarylphenol polyalkoxyether sulfates and phosphates include, for example, SOPROPHOR® 4 D 384 (tristyrylphenol (EO)16 sulfate ammonium salt), SOPROPHOR® 3 D 33 (tristyrylphenol (EO)16 phosphate free acid), Sophrophor BSU (Tristyryl phenol ethoxylate), SOPROPHOR® FLK (tristyrylphenol (EO)16 phosphate potassium salt) and SOPROPHOR® RAM/384 (tristyrylphenol polyethoxylated ether sulfate neutralized with polyethoxylated oleylamine). In other embodiments, the polyarylphenol polyalkoxyether sulfates and phosphates may be mono-arylphenol polyalkoxyether sulfates and phosphates, such as styrylphenol polyethoxyether sulfates and phosphates.

Exemplary C8-18 alcohol polyethoxyether phosphates, carboxylates and citrates include STEPFAC® 8180 (tridecylalcohol (EO)3 phosphate), STEPFAC® 8181 (tridecylalcohol (EO)6 phosphate), STEPFAC® 8182 (tridecylalcohol (EO)12 phosphate), EMCOL® CN-6 (tridecylalcohol (EO)6 carboxylate). The C8-18 alcohol polyethoxyether phosphates, carboxylates and citrates may have a degree of ethoxylation of between 1 and 25, preferably between 1 and 20.

Exemplary alkylbenzene sulfonic acids and salts thereof include dodecylbenzene sulfonic acid, and metal (for example sodium or calcium, Atlox™ 4838B), ammonia or amine salts of the alkylbenzene sulfonic acids, including dodecylbenzene sulfonic acid. Amine neutralized versions include primary amines, diamines, triamines and alkanol amines.

Additional preferred anionic surfactants include (C8-12 alkyl)phenol polyoxyethylene ether sulfates, and (C8-12 alkyl)phenol polyoxyethylene phosphate mono- and diesters, accompanied in each case by monovalent counterions. In one embodiment the monovalent counterion for a (C8-12 alklyl)phenol polyoxyethylene ether sulfate or a (C8-12 alkyl)phenol polyoxyethylene phosphate is a protonated polyoxyethylene C12-20 alkylamine surfactant. More specifically, polyoxyethylene tallowamine salt of a nonylphenol polyoxyethylene ether sulfate, nonylphenol polyoxyethylene phosphate, and a blend of such nonylphenol polyoxyethylene phosphate with polyoxyethylene tallowamine.

The composition of the present invention may also include non-ionic surfactant as additional emulsifier, which is a water-soluble, 20 to 250 ethylene glycol ether groups containing polyadducts of ethylene oxide and propylene oxide, ethylene diamino polypropylene glycol and alkyl polypropylene glycol with 1 to 10 carbon atoms in the alkyl moiety, the substances normally contain 1 to 5 ethylene glycol units per propylene glycol unit. Examples of non-ionic surfactants are nonylphenol polyethoxy ethanols, vegetable oil polyglycol ethers, polyadducts of ethylene oxide and propylene oxide, tributyl phenoxy polyethoxy ethanol (Rthylan NS 500), octyl phenoxy polyethoxy ethanol.

The emulsifier is present in the composition in a range of 1% to 30%, more preferably 2% to 20% of the total weight of the composition.

Solvent as used herein is a suitable water-immiscible solvents selected from the group consisting of aromatic hydrocarbons, aliphatic hydrocarbons, dialkylene glycol dialkyl ethers (diethylene glycol dimethyl ether), dimethyl sulfoxide. ketones, alkyl esters of acetic acid or mixtures thereo. Aromatic and aliphatic hydrocarbons such as hexane, cyclohexane, benzene, toluene, xylene, mineral oil or kerosene or substituted naphthalenes, polar aprotic solvents such as DMSO, DMF etc, mixtures of mono- and polyalkylated aromatics are commercially available under the registered trademarks Solvesso® and Shellsol® and Petrol Spezial®.

The solvents are present in the composition in a range of 1% to 70%, more preferably 30% to 70% of the total weight of the composition.

Nitenpyram and Pyriproxyfen may be present in the composition or applied in any amounts relative to each other, to provide the enhanced or synergistic effect of the mixture. Nitenpyram and Pyriproxyfen may be present in the synergistic pesticidal composition in any suitable amount, and is generally present in an amount of from 1% to 50% by weight of the composition, preferably from 1% to 40% by weight of the composition. However, variables of the ratios of each of the active ingredients can be used and the selection of the particular amount is dependent upon many factors including, for example, type of formulations, the crop, disease sought to be controlled and environmental conditions. The selection of the proper quantity of active agent to be applied, however, is within the expertise of one skilled in the art.

Formulation includes water soluble concentrate (SL), an emulsifiable concentrate (EC), an emulsion (EW), a microemulsion (ME), a suspension concentrates (SC), an oil-based dispersions (OD), a flowable suspension (FS), water soluble powder (WS), water-dispersible granule (WDG), water-soluble granule (SG), a water-dispersible powder (WP), a water soluble powder (SP), a granule (GR), an encapsulated granule (CG), a fine granule (FG), a macrogranule (GG), an aqueous suspoemulsion (SE), capsule suspension (CS) and a microgranule (MG). Preferably, the synergistic pesticidal composition can be formulated as emulsifiable concentrate (EC).
The EC compositions of the present invention can be diluted with water or water solutions of agronomic compounds before use to produce a sprayable suspension composition which is used in treating plants or increasing plant growth, Dilution in water usually results in suspensions, emulsions, suspoemulsions or solutions of the agrochemical active ingredient at a concentration of at least 0.001 g/l.

A particular mode of administering the composition of the present invention is the administration to the aboveground parts of plants, in particular to the leaves thereof (leaf-application). The number of applications and the administered doses are chosen in accordance with the biological and climatic conditions of life of the causative agent. The antifungal compositions though, can also be applied to the soil and get into the plants through the root system (systemic activity), in case the locus of the plants is sprayed with a liquid composition or if the components are added to the soil in a solid formulation e.g. in the form of wettable powder (soil application).

When using the active compound combinations according to the invention, the application rate can be varied within a relatively wide range, depending on the kind of application. For the treatment of parts of plants, the active-compound combination application rates are generally between 0.1 and 10000 g/ha, preferably between 100 and 2500 g/ha. For seed dressing, the active compound combination application rates are generally between 0.001 and 50 g per kilogram of seed, preferably between 0.01 and 20 g per kilogram of seed. For the treatment of the soil, the active-compound combination application rates are generally between 0.1 and 10000 g/ha preferably between 1 and 5000 g/ha.

The following examples describes the nature of the invention which are given only for the purpose of illustrating the present invention in more detail and are not limitative and relate to solutions, which have been particularly effective on bench scale.

EXAMPLES

Example 1: Composition of Nitenpyram 15% and Pyriproxyfen 20% EC:
S. No. Ingredients/Raw Materials Quantity (% w/w)
1. Nitenpyram technical @ 93.75 b)15% 16
2. Pyriproxyfen technical @ 94.2 b) 20% 21.23
3. Dimethyl Sulfoxide 18
4. Diethylene glycol Dimethyl ether 34.77
5. Alkoxylated butyl ether EO-PO (Ethylan NS 500) 5
6. Tristyryl phenol ethoxylate (Sophrophor BSU) 5
Total 100

Manufacturing process

All the raw materials were weighed dispensed and the mixture was mixed in a homogenizer for 60 minutes. The mixture was passed through filter cloth and the material was collected and packed.

Field efficacy trials for Example 1

Trial 1: Cotton
Cotton is one of the most grown commercial crops of India. It grows well in almost all areas of the country but mainly grown in nine states major states which includes North India, Central India, and South India. It is a member of Malvaceae family. However, the cultivation of cotton possesses a great threat starting from planting up to the harvest of bolls due to the attack of enormous pest and disease complex. Among all the pests, sucking pests are many and they cause a huge loss by affecting the vegetative growth of the plants. Sucking pest complex is enormously important.

To reduce losses in sucking pests, field efficacy trials were conducted in cotton on sucking pest complex viz., Leafhopper, Amrasca biguttula biguttula (Ishida), Aphid, Aphis gossypii (Glover), Whitefly Bemisia tabaci (Gennadius) and Thrip, Thrips tabaci (Linnman) which cause severe damage.

Trials were conducted to evaluate the efficacy of innovative mixtures of Nitenpyram and Pyriproxyfen, these trials were conducted with randomized block design with net plot size of 5m x 6m. Cotton crop was raised with all standard agronomic practices. Spraying was done with manual operated backpack sprayer with 500 liter of water spray volume per hectare at 60 days after sowing. The observations were recorded by counting the No. of sucking insects (Whitefly adult and Nymph, Jassids and Thrips) per leaf, six leaves per plant and five plants per plot. The observations were recorded before spraying and 10 DAA (Days after application).

Table 1: Sucking Pests control in Cotton

Compositions
Dose Gram Active/hectare % Insect Control at 10 DAA

Whiteflies adults
Wflies Nymphs
Thrips
Jassids
Aphids
Nitenpyram 15% + Pyriproxyfen 20% EC (Example 1) 60+80 90.6 92.8 90.6 95.2 93.8
Nitenpyram 25% SP 60 32.0 33.8 37.9 45.3 43.8
Pyriproxyfen 10% EC 80 52.8 56.2 42.1 39.6 37.8
Nitenpyram 15% + Pyriproxyfen 20% EC 75+100 94.8 95.2 93.8 97.8 96.0
Nitenpyram 25% SP 75 35.5 36.9 38.9 47.6 44.5
Pyriproxyfen 10% EC 100 53.7 56.9 45.3 42.5 40.8
SP- Soluble powder, WP Wettable Powder, EC Emulsifiable Concentrate, DC Dispersible concentrate DAA Days after Application

Results given in Table No 1, shows excellent efficacy which include the combination insecticide Nitenpyram +Pyriproxyfen against sucking pest complex Thrips, Jassids and Aphids.

The solo application as well as the combination of two active ingredients tested here against Thrips, Jassids and Aphids. Solo product application was not able to provide satisfactory control of Thrips, Jassids and Aphids, whereas synergistic effect has been seen in combination Nitenpyram + Pyriproxyfen EC composition in controlling sucking pests complex, which has excellent efficacy as well as residual control.

Trial 2 - Tomato Pests:

Tomato (Solanum lycopersicon L.) is one of the most popular and widely grown solanaceous crop in many countries including India. The estimated area under tomato in India is about 80.85 lakh ha with a production of 19.69 lakh tonnes and productivity 24.4 MT/ha of fruits.

Tomato growers regularly experienced the economic damage caused tomato Fruit borer (Helicoverpa armigera Hubner), Leaf miner (Liriomyza trifolii Blanchard), Thrips (Thrips tabaci L.), White flies (Bemisia tabaci Gennadius), Cutworm (Agrotis ipsilon Root), Leaf eating caterpillar (Spodoptera litura Fabr).

The field trials were conducted to evaluate the efficacy of innovative mixtures of Nitenpyram + Pyriproxyfen against sucking pests of Tomato viz., Whitefly, Bemisia tabaci, Aphid, Toxoptera sp. Thrips, Thrips tabaci, F. rankliniella which cause severe damage.

Trials were conducted with randomized block design with net plot size of 5m x 6m. Tomato crop was raised with all standard agronomic practices. Spraying was done with manual operated backpack sprayer with 500 liter of water spray volume per hectare at 50 days after sowing. The observations were recorded by counting the no. of sucking insects (Whitefly adults, Nymphs, and Thrips) per leaf, six leaves per plant and five plants per plot. The observations were recorded before spraying and 10 DAA (Days after application).

Table 2: Sucking Pest control in Tomato

Active Dose (GAH) % Insect Control at 10 DAA
Whiteflies adults Wflies Nymphs Thrips
Nitenpyram 15% + Pyriproxyfen 20% EC 60+80 88.32 89.8 85.78
Nitenpyram 25% SP 60 36.5 37.4 35.8
Pyriproxyfen 10% EC 80 55.6 59.5 45.5
Nitenpyram 15% + Pyriproxyfen 20% EC 75+100 91.8 92.5 87.9
Nitenpyram 25% SP 75 39.8 40.5 37.0
Pyriproxyfen 10% EC 100 56.2 60.8 47.5
SP- Soluble powder, WP Wettable Powder, EC Emulsifiable Concentrate, DC Dispersible concentrate DAA Days after Application

The trial results given in Table No. 2, has shown excellent efficacy of Nitenpyram +Pyriproxyfen EC Insecticide combination against Whiteflies (Adult and Nymph) and Thrips. The solo application as well as the combination of two active ingredients tested here against Whitefly nymphs and adults, Thrips. Solo products was not able to provide satisfactory control of Whiteflies and also for Thrips, whereas the synergistic effect has been seen of Nitenpyram +Pyriproxyfen EC combination against Whiteflies and Thrips, which has also shown excellent efficacy as well as residual control.

,CLAIMS:
WE CLAIM:
1. An emulsifiable concentrate composition comprising combination of Nitenpyram and Pyriproxyfen at a weight ratio of 1 : 5 to 5 : 1 of first active ingredient to the second active ingredient, emulsifiers and other agrochemical auxiliaries.

2. The emulsifiable concentrate composition as claimed in claim 1, wherein said composition comprises:
1% to 30% (w/w) of Nitenpyram as first active ingredient,
1% to 40% (w/w) of Pyriproxyfen as second active ingredient,
1% to 30% (w/w) of emulsifiers, and
0.1% to 70% (w/w) of other additives / auxiliaries.

3. The emulsifiable concentrate composition as claimed in claims 1 and 2, wherein said composition comprises:
1% to 30% (w/w) of Nitenpyram,
1% to 40% (w/w) of Pyriproxyfen,
1% to 30% (w/w) of emulsifiers,
1% to 70% (w/w) of solvents, and optionally
10% to 20% (w/w) of inert carrier or fillers.

4. The emulsifiable concentrate composition as claimed in claims 1 to 3 wherein said composition comprises:
1% to 30% (w/w) of Nitenpyram,
1% to 40% (w/w) of Pyriproxyfen,
1% to 10% (w/w) of alkoxylated butyl ether EO-PO,
1% to 20% (w/w) of tristyryl phenol ethoxylate,
1% to 30% (w/w) of dimethyl sulfoxide, and
1% to 40% (w/w) of diethylene glycol dimethyl ether.

5. The emulsifiable concentrate composition as claimed in claims 1 to 2, wherein said emulsifier is selected from polyarylphenol polyalkoxyether sulfates and/or phosphates; C8-18 alcohol polyalkoxyether phosphates, carboxylates, and/or citrates; alkyl benzenesulfonic acids; C8-20 alkyl carboxylates including fatty acids; C8-20 alcohol sulfates; C8-20 alcohol phosphate mono- and diesters; C8-20 alcohol and (C8-20alkyl)phenol polyoxyethylene ether carboxylates, sulfates and sulfonates; C8-20 alcohol and (C8-20 alkyl)phenol polyoxyethylene phosphate mono- and diesters; C8-20 alkylbenzene sulfonates, naphthalene sulfonates and formaldehyde condensates thereof; lignosulfonates; C8-20 alkyl sulfosuccinates and sulfosuccinamates; C8-20 acyl glutamates, sarcosinates, isethionates and taurates; water-soluble soaps.

6. The emulsifiable concentrate composition as claimed in claims 1 to 2, wherein said solvent is selected from aromatic hydrocarbons, aliphatic hydrocarbons, diethylene glycol dimethyl ether, dimethyl sulfoxide, ketones, alkyl esters of acetic acid or mixtures thereof; aromatic and aliphatic hydrocarbons such as hexane, cyclohexane, benzene, toluene, xylene, mineral oil or kerosene or substituted naphthalenes, polar aprotic solvents such as DMSO, DMF etc, mixtures of mono- and polyalkylated aromatics commercially available under the registered trademarks Solvesso® and Shellsol® and Petrol Spezial®.

7. The process for the preparation of emulsifiable concentrate composition as claimed in claim 2, wherein said process comprising the steps of:
a. weighing all the active ingredients,
b. mixing all the ingredients in homogenizer, and
c. filtering the obtained material and packing.

8. The process for the preparation of emulsifiable concentrate composition as claimed in claims 4 and 7, wherein said process comprising the steps of:
a. weighing and mixing Nitenpyram, Pyriproxyfen, alkoxylated butyl ether EO-PO, tristyryl phenol ethoxylate, dimethyl sulfoxide and diethylene glycol dimethyl ether, and
b. filtering the obtained material and packing.

Dated this Twenty Ninth (29th) day of July, 2021

__________________________________
Dr. S. Padmaja
Agent for the Applicant
IN/PA/883