A process of refolding a recombinant protein from inclusion bodies (IBs) formed inside a host cell, is provided. The process includes homogenising a wet cells slurry to obtain a cell lysate; incubating the cell lysate with a reducing agent to obtain a reduced cell lysate; isolating reduced IBs from the reduced cell ly...

An expression vector for production of a recombinant protein in a host cell is provided. The expression vector includes a nucleotide sequence of Sequence ID No 2 encoding for a leader peptide of sequence ID No 3.

A process for production of insulin or insulin analogues by expression of Insulin or Insulin analogues through an expression vector in a host cell is provided. The expression vector includes a leader peptide of SEQ ID NO 3; a nucleotide sequence encoding an affinity tag linked to C-terminal end or N terminal end of nu...

A machine or apparatus for refolding a protein of interest produced recombinantly in a host cell in form of inclusion bodies is provided. The apparatus includes an inclusion bodies (IB) solubilisation tank to solubilise the inclusion bodies; a plurality of refolding vessels or reactors arranged in series to receive ...

ABSTRACT A method or assay to detect E.coli host cell protein (HCP) in biopharmaceutical cultures or samples comprising of the addition of an antibody specific to host cell protein to form an anti-E.coli HCP antibody-HCP conjugate; the addition of a labelled detection antibody which binds to the anti-HCP antibody-H...

An expression vector for production of insulin or insulin analogues in a host cell is provided. The expression vector includes a leader peptide of SEQ ID NO 3; a nucleotide sequence encoding an affinity tag linked to C-terminal end or N terminal end of nucleotide sequence of the leader peptide; and a nucleotide sequ...

An expression vector for production of a recombinant protein in a host cell is provided. The expression vector includes a nucleotide sequence of Sequence ID No 2 encoding for a leader peptide of sequence ID No 3.

A method or assay to detect porcine trypsin in biopharmaceutical cultures or samples comprising of the addition of an antibody specific to porcine trypsin to form an anti-trypsin antibody-trypsin conjugate; the addition of a labelled detection antibody which binds to the anti-trypsin antibody-trypsin conjugate and fo...