Provided are computer implemented systems and methods for detecting one or more analytes in an immunoassay. Embodiment systems and methods of the disclosure can train a machine learning network using immunoassay reference data on an analyte and output a set of target experimental input parameters to detect the analy...

Disclosed herein are isolated polypeptides and antibodies with improved sensitivity and decreased background signals and methods of using the same. Specifically, the antibodies comprise polypeptides comprising a sequence-defined CH2 domain linked to hinge domain.