The patent is related to the process for the purification of Pneumocandin B0, a secondary metabolite obtained from fermentation of anamorphic fungi Glarea lozoyensis. Pneumocandin B0 is a key intermediate for potent antifungal drug Caspofungin Acetate.

The present invention provides a stable genetic mutant, Glarea lozoyensis JCCC-1097 and process of using the strain for production of Pneumocandin B0 in high yield with reduced level of structural analogues especially Pneumocandin C0