ABSTRACT AN ARTIFICIAL INTELLIGENCE METHOD TO PREDICT SELECTIVITY OF TRANSAMINASES AND ENANTIOMERIC EXCESS OF TRANSAMINASE-KETONE REACTIONS. The present invention describes identifying if a given Transaminase is (R)-Selective or (S)-Selective. Based on the binding of the substrate in the active site of a given tran...

The invention describes the methods of directing CRISPR complex formation in bacterial cells and utilizing the CRISPR-Cas system to edit a gene of interest in a plasmid. An AI incorporated CRISPR tool to engineer enzymes for better activity and allow cells to undergo specific and random mutation for a variety of res...

USE OF ENGINEERED CYTOSKELETAL AND STRUCTURAL PROTEINS TO FUNCTION AS ENZYMES AND ENZYME IMMOBILISATION MEDIA. Nitrilases can hydrolyse nitriles into their corresponding carboxylic acids and ammonia, but its applications in processes have been severely hampered by their labile and unstable nature. The lack of purifi...

ENGINEERING OF PROTEINS TO HAVE MULTIPLE ACTIVE SITES TO CATALYZE MULTIPLE REACTIONS. The engineering of proteins to include multiple active sites for catalyzing various reactions represents a significant advancement in enzyme engineering. Currently, most industrial enzymatic processes involve enzymes that catalyze ...

The present invention provides a method for synthesizing nicotinic acid from 3-picoline using transformed recombinant host cells with synthetically designed gene constructs as whole cell biocatalysts. Adaptive engineering of aromatic ring metabolizing genes isolated from microorganisms enables efficient metabolism o...

A METHOD FOR ENGINEERING PROTEINS. Presented herein is a method for protein engineering that constructs a three-dimensional gridspace around a protein. Different probes, simulating the interactions of amino acids are placed within the grid. Pair Interaction Energy is calculated using FMO technique for probe-protein ...

The present invention provides an engineered nitrilase polypeptide designed to convert (1-cyanomethyl)cyclohexane-1-carbonitrile into (1-cyanocyclohexyl)acetic acid. Using a conglomeration of efficient enzyme engineering methods, the nitrilase has been engineered to enhance its stability and activity compared to nat...

ABSTRACT METHOD FOR CAPTURING ATOMIC DETAILS OF PROTEINS USING A 3D GRID FOR MUTATIONAL ANALYSIS. This invention presents a novel method for engineering glucose dehydrogenase (GDH) proteins by utilizing an atomistic grid-based computational method that analyzes and compares protein atomic compositions. The method i...

ABSTRACT ENGINEERED CYTOSKELETAL FTSZ POLYPEPETIDE WITH NITRILASE ACTIVITY. Nitrilases can hydrolyse nitriles into their corresponding carboxylic acids and ammonia, but its applications in processes have been severely hampered by their labile and unstable nature. The lack of purified enzyme preparations has prompted...

ENGINEERED LEUCINE DEHYDROGENASE AND APPLICATIONS THEREOF. Leucine dehydrogenase is a NAD+ dependent oxidoreductase, engineered to perform reductive amination and oxidative deamination. The engineered Leucine dehydrogenase used to prepare amino acids from the keto acid through reductive amination and L-amino acids t...

ENGINEERED FORMATE DEHYDROGENASES AND USES THEREOF. Formate dehydrogenases (FDHs) are widely utilized in biotransformation processes, particularly in the regeneration of NAD(P)+/NAD(P)H-dependent oxidoreductases. These enzymes are essential for the commercial synthesis of chiral chemicals, but they suffer from the d...

ENGINEERED THREONINE DEAMINASE FOR AMINO TRANSFERASE ACTIVITY. Threonine deaminases catalyze the deamination process, which is crucial for synthesizing unconventional a-keto acids that serve as precursors for various amino acids and pharmaceutical intermediates. However, the application of natural threonine deaminas...