The invention relates to a mutant α-hemolysin (α-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically...

The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is...

A piston head for a syringe pump comprises a barrier portion (11) for driving fluid through a syringe pump barrel (30), wherein a peripheral section (13,14) of the barrier portion (11) is shaped to seal against the syringe pump barrel (30); and a resilient member (15) arranged to resist deformation of the shaped per...

An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is...

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a RecD helicase. The helicase controls the movement of the target polynucleotide through the pore.

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and an XPD helicase. The helicase controls the movement of the target polynucleotide through the pore.

An apparatus for supporting an array of layers of amphiphilic molecules the apparatus comprising: a body (11) formed in a surface of the body an array of sensor wells (10) capable of supporting a layer of amphiphilic molecules (30) across the sensor wells the sensor wells each containing an electrode (12) for connec...

A time ordered series of measurements of a polymer made during translocation of the polymer through a nanopore are analysed. The measurements are dependent on the identity of k mers in the nanopore a k mer being k polymer units of the polymer where k is a positive integer. The method involves deriving from the serie...

The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses apta...

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to s...

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.

The invention relates to mutant forms of Msp. The invention also relates to nucleic acid characterisation using Msp.

The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providi...

The invention relates to constructs comprising a nucleic acid binding protein and a surface. At least one native accessible cysteine residue is removed from the binding protein. The binding protein is attached to the surface via one or more accessible cysteine residues. The removal of other accessible cysteine re...

The invention relates to a new method of determining the presence absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

An apparatus for sensing of an interaction of a molecular entity with a membrane protein in a lipid bilayer comprises an array of sensor elements (21) arranged to output an electrical signal that is dependant on occurrences of the interaction. A detection circuit (3) comprised detection channels (30) capable of ampl...

To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of...

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide thro...

A pump for use in low profile applications comprises a barrel (30) for holding fluid; and a piston (20) that converts a rotational driving force into a longitudinal driving motion within the barrel. The pump provides space saving advantages by reducing the need for external equipment and mechanisms around the pump f...

A sequence of polymer units in a polymer (3) eg. DNA is estimated from at least one series of measurements related to the polymer eg. ion current as a function of translocation through a nanopore (1) wherein the value of each measurement is dependent on a k mer being a group of k polymer units (4). A probabilistic m...

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a...

Apparatus and methods for controlling the insertion of a membrane channel into a membrane are disclosed. In one arrangement a first bath holds a first liquid in contact with a first surface of a membrane. A second bath holds a second liquid in contact with a second surface of the membrane. The membrane separates the...

A microfluidic device for analysing a test liquid comprises: a sensor (235), such as a membrane provided with nanopores, provided in a sensing chamber (237); a sensing chamber inlet channel (261) and a sensing chamber outlet channel (262), each connecting to the sensing chamber for respectively passing liquid into a...