Background: PCR based diagnosis for visceral leishmaniasis is far from being applied in field, despite numerous published primers. Although the technique is simple, selection of appropriate primer is crucial for the success of the investigative technique. The present study was planned to design a Leishmania specific...

In regions endemic for Visceral leishmaniasis (VL), asymptomatic infection is common and only a small proportion of persons infected with L.donovani develop clinical VL, characterized by febrile splenomegaly and pancytopenia. To detect infection in healthy persons, the Montenegro test or Leishmania skin test (LST) i...