DESC:TECHNICAL FIELD
The present invention relates to a composition for improving soil, particularly the present invention relates to a multienzyme mix based composition for soil drenching and a method for preparing the same.
BACKGROUND
Soil quality is imperative for growing improved vegetation that can resist pests and diseases and yield plentifully, but also function as a vital living system, within ecosystem and land-use boundaries, to sustain diverse biological productivity, promote the quality of air and water environments, and maintain plant, animal, and human health. Improving soil quality is a continuing process and currently, many chemical fertilizers are widely used in agriculture to boost crop yields and improve soil fertility. However, they have several limitations affecting our ecosystem and thus the economy.
Chemical fertilizers primarily cause soil degradation and pollution. Many chemical fertilizers contain high levels of nitrogen salts, phosphorus, and other chemicals that can leach into groundwater and surface water, causing eutrophication and algal blooms. This predominantly leads to oxygen depletion and may negatively impact aquatic life. Further, chemical fertilizers can deplete soil organic matter harming the microbes present in the soil, reducing soil fertility, and changing the nature of soil by making it either too acidic or too alkaline, making it less conducive to plant growth.
Chemical fertilizers often have heavy metals such as Lead, Mercury, Cadmium, and Uranium, that can harm human health, causing damage to the kidneys, liver, and lungs. In addition, these heavy metals are also associated with other human health hazards.
Most significantly, chemical fertilizers come at a high cost and yield short-term benefits. The utilization of chemical fertilizers in recent years has led to contamination of waterways, chemical damage to crops, intensified air pollution, soil acidification, and depletion of soil minerals. This results in diminishing returns on investment, posing risks of crop failure, pest infestations, and soil erosion. Additionally, chemical fertilizers are easily washed away by water, contributing to pollution, and adversely impacts soil-dwelling microbes, ultimately reducing soil fertility. Additionally, their expensive nature presents a major disadvantage. Moreover, these fertilizers provide temporary benefits. Finally, they have the potential to modify soil composition, leading to an undesirable shift towards either excessive acidity or alkalinity.
Biofertilizers have emerged as an alternative as they can significantly replace chemical nitrogen and phosphorus, stimulate plant growth, activate the soil biologically, and naturally restore soil fertility among many other such benefits compared to the existing chemical fertilizers. Many methods are followed in biofertilizers applications such as soil application, improvement of seeds and seedlings drenching, and foliar spray. Although, biofertilizer application through drenching process ensures direct contact with plant parts and effective in rapid usage of biochemicals for instantaneous results, many drenching mixtures are dependent on factors such as plant location, variety, and plant product characteristics such as solubility, absorption and translocation.
Thus, there is a need for developing new compositions to the existing chemical and biofertilizers for promoting plant growth, crop yield and to overcome the abovementioned problems.
SUMMARY
The present disclosure relates to a discloses a composition for soil drenching comprising a multienzyme mixture with 99 – 99.9 w/w of multienzymes and 0.1 - 1% of agriculturally accepted excipients. The multienzymes further comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase.
According to one aspect of present disclosure, is to provide the composition in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray, oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable, concentrate, nano emulsions, water dispersible granules, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion, soluble powder, wettable powder, an encapsulated granule and the like.
According to another aspect of present disclosure the multienzyme mixture is selected from animals, plants, insects, or biological extracts.
According to one aspect of present disclosure, the agriculturally accepted excipients comprise malt dextrose and enzyme stabilizer.
According to another aspect of present disclosure, the composition for drenching is applied to soil after sowing of crops that are grown at a temperature range of 10 °C to 55°C.
According to one aspect of present disclosure is to develop a method of preparing composition for drenching for soil application, wherein the method comprising: (i) weighing enzyme powders, mixing thoroughly in suitable mixer machines; (ii) preparing multienzyme mixture comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase; (iii) adding suitable agriculturally accepted excipients selected from a group comprising plant growth hormones, preservatives, anti-foaming agents, surfactants, dispersing agents, wetting agents, suspension agents, thickeners, binders, emulsifiers, and the like; and (iv) stabilizing the pH of the mixture to a range of 4.5 to 8 to obtain the composition for soil drenching.
According to another aspect of present disclosure, the obtained composition is in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray, oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable, concentrate, nano emulsions, water dispersible granules, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion, wettable powder, an encapsulated granule and the like.
According to one aspect of present disclosure is to select the enzymes from animals, plants, insects, or biological extracts.

According to one aspect of present disclosure, the composition is applied to soil, plant, crops, leaf, seeds, seedlings, or roots growing at a temperature range of 10 °C to 55°C.
According to another aspect of present disclosure, the application of the enzyme mixture to the soil has increased its nutritional value and improved the uptake of nutrients by both soil microbes and plants, resulting in higher quality and higher yield crop production. The enzyme mix also has the potential to enhance soil texture, solubilize organic and inorganic components, release essential nutrients like phosphorus, nitrogen, sugars, macro and micronutrients, and trace elements, balance the absorption of nitrogen, phosphorus, and potassium, improve crop stress resistance, and ultimately lead to improved or enhanced crop yield and quality.
According to one aspect of present disclosure, the multienzyme mixture comprising 99 – 99.9 w/w of multienzymes further comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase, along with suitably added agriculturally added excipients show improved activity.
These and other aspects of the embodiments herein will be better appreciated and understood when considered in conjunction with the following description and the accompanying drawings. It should be understood, however, that the following descriptions, while indicating preferred embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.
BRIEF DESCRIPTION OF DRAWINGS
The above and still further features and advantages of embodiments of the present disclosure become apparent upon consideration of the following detailed description of embodiments thereof, especially when taken in conjunction with the accompanying drawings, and wherein:
Figure 1 illustrates Effect of F1 on greenness of leaves using Leaf Color Chart (LCC) for Paddy crop.
Figure 2 illustrates Effect of F1 on shoot and root length of Paddy.
Further to facilitate understanding, reference numerals have been used, where possible, to designate elements common to the figures.
DETAILED DESCRIPTION
Various aspect of the present disclosure provides a composition for improving soil and crop health. The following description provides specific details of certain aspects of the disclosure illustrated in the drawings to provide a thorough understanding of those aspects. It should be recognized, however, that the present disclosure can be reflected in additional aspects and the disclosure may be practiced without some of the details in the following description.
The various aspects including the example aspects are now described more fully with reference to the accompanying drawings, in which the various aspects of the disclosure are shown. The disclosure may, however, be embodied in different forms and should not be construed as limited to the aspects set forth herein. Rather, these aspects are provided so that this disclosure is thorough and complete, and fully conveys the scope of the disclosure to those skilled in the art. In the drawings, the sizes of components may be exaggerated for clarity.
The subject matter of example aspects, as disclosed herein, is described with specificity to meet statutory requirements. However, the description itself is not intended to limit the scope of this patent. Rather, the inventor/inventors have contemplated that the disclosed subject matter might also be embodied in other ways, to include different features or combinations of features similar to the ones described in this document, in conjunction with other technologies. Generally, the various aspects including the example aspects relate to a composition for improving soil fertility.
As mentioned, there is a need for improved composition that are alternative to the microbes that promotes plant growth, improve crop yield. The present aspect, therefore, provides an multienzyme composition to promote plant health and improve crop yield, The present disclosure also provides a method for preparation of the same.
The enzymes and agriculturally accepted excipients used in the present disclosure for preparing the compositions are commercially available (Ultreze Enzymes Private Ltd & Advanced Enzymes Pvt Limited, India) and are accordingly procured. Further, the agriculturally accepted excipients comprise malt dextrose and enzyme stabilizer.
As used in here, the term “seed germination” refers to, but not limited to, the process of development of a new plant from a dormant embryo within the seed under the availability of suitable conditions like temperature, water, air, and light, resulting in the rupture of the seed coat.
As used in here, the term “crop vigour” refers to, but not limited to, the combined factors of the seed that influence its potential level of activity and performance during germination and seedling emergence.
As used in here, the term “Plant height” refers to, but not limited to, the distance from the upper boundary of the main photosynthetic tissues (excluding inflorescences) on a plant to the ground level.
As used in here, the term “Root weight and shoot weight” refers to, but not limited to, the proportion of a plant's biomass that is allocated to its root and shoot system respectively.
As used in here, the term “Tillers count” refers to, but not limited to, the total tiller number per metre row of crop.
As used in here, the term “Productive tiller count or number” refers to, but not limited to, the quantity of tillers that are responsible for the production of spikes and seeds. Typically, the assessment of productive tiller number is conducted during the maturity phase of the crop.
As used in here, the term “panicle length” refers to, but not limited to, the length from the panicle neck to the panicle tip of the main panicle.
As used in here, the term “panicle weight” refers to, but not limited to, the weight of panicle from each plant.
As used in here, the term “Stem girth” refers to, but not limited to, the thickness or width of the stem.
As used in here, the term “Drenching” refers to, but not limited to, a method or practice utilized to apply specific formulations, such as dry, slurry, liquid, or others, to the intended plant material or soil, for a specific duration, while ensuring the pH level and temperature is suitable for the needs.
As used in here, the term “Broadcasting or Broadcast planting” refers to, but not limited to, a technique used for sowing seeds by dispersing them across the soil. The seeds are distributed over the soil through mechanical or manual methods. Initially, the seeds are evenly scattered and subsequently covered with planking. Furthermore, the application of broadcasting has been employed to disperse powdered enzyme formulations across paddy fields by blending them with soil or sand in order to enhance their quantity and envelop the complete acreage.
In an embodiment, the present disclosure provides a composition for soil drenching, comprising a multienzyme mix (a) 99 – 99.9 w/w of multienzymes further comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase; and (b) 0.1 - 1% of agriculturally accepted excipients.
In another embodiment, the enzyme xylanase used in the multienzyme mixture for drenching aids in converting stored food into a form that can be used by the plant. They are essential for cell elongation and the ripening of fruits, carrying out various important functions in these processes.
In an embodiment, the enzyme laccases used in the multienzyme mixture for drenching play a crucial role in the oxidation of various (poly) phenolic compounds such as lignin and thus contributing to delignification and enhancing recalcitrant soil organic matter (SOM) turnover, ultimately resulting in increased soil nutrition values.
In an embodiment, the enzyme phytase used in the multienzyme mixture for drenching increase phosphorus levels in soil, which is essential for crop growth. These enzymes break down organic phosphorus compounds in soil, releasing inorganic phosphates that can be used by plants and soil organisms. It is important to note that a high concentration of phytate is not necessary for seed or plant growth, as phytase enzymes help break down phytin during germination to release nutrients for the plants. The phytase enzymes in the multienzyme mixture play a key role in breaking down phytate into inorganic phosphate and reducing myo-inositol levels. The presence of high levels of phytate is not required for seed or plant survival, as phytase enzymes aid in nutrient release during germination.In another embodiment, the cellulases and endo glucanase used in the multienzyme mixture for drenching may promote availability of nutrients to the soil. Adding organic matter to the soil in the form of plant residues and litter may give high amounts of cellulose, group of multienzymes involved in the decomposition of cellulose such as cellulases, and glucose polymers. Cellulases and glucanase are significant soil multienzymes that can decompose the cellulase and make it available to soil microbial community in the form of nutrients. Most plant grains typically consist of approximately 70-80% starch. The function of these enzymes in the process of starch hydrolysis is crucial for seed germination. The enzyme ß-glucanase used in the multienzyme mixture for drenching play important roles in formation of intermediates during cell wall lignification, cell wall degradation in the endosperm during germination and activation of phytohormones and activation of chemical defenses. Also, the enzyme cellulase used in the multienzyme mixture for drenching primarily helps in the repair and organization of cellulose microfibrils during the production of cellulose in plants life and also in hydrolyzing stored carbohydrates to provide energy for germination and establishment of plants.
In another embodiment, the proteases used in the multienzyme mixture for drenching may increase the concentration of nitrogen in the soil and may further enhance the plant growth. Protease is a soil multienzyme important in the mineralization of organic nitrogen content in the soil. They hydrolyze proteins to amino acids, that can act as nitrogen sources for the soil microbes or mineralize to release ammonia. The breakdown of seed protein during seed germination is a crucial metabolic process that involves multiple steps and is facilitated by Proteolytic enzymes or proteases. This process involves the hydrolysis or catalysis of stored seed proteins, leading to the release of free amino acids. These liberated amino acids play a vital role in the synthesis of proteins within the endosperm and embryo, thus priming the germination process.
In an embodiment, the lipase used in the multienzyme mixture for drenching may convert lipids in soil organic matter, allowing release of bio-available nutrients for the plant, and stimulating natural microbial soil activity. Lipase breaks down lipids in soil organic matter, releasing nutrients that can be used by plants and promoting microbial activity in the soil.
In another embodiment, the composition for soil drenching may further include one or more agriculturally accepted excipients such that the one or more agriculturally accepted excipients may be selected from one or more of, a carrier, binder, thickener, emulsifiers, adjuvants, surfactants, coating agents, suspending agents, stabilizers, wetting agents, gelling agents, and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of excipients, including known, related, and later developed technologies.
In an embodiment, the source of multienzymes used in the multienzyme mixture for soil drenching is selected from a group, but not limited to, animal, plants, insects, microbes, or biological extracts.
In another embodiment, the emulsifier used in the composition for soil drenching may be selected from a group, but not limited to, an ethoxylated alcohols, polyalkoxylated butyl ethers, calcium alkyl benzene stilphonates, polyalkylene glycol, polyoxyethylenesorbitan esters, polyoxyethylenesorbitanmonolaurate and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of emulsifier, including known, related, and later developed technologies.
In an embodiment, the binder used in the composition for soil drenching may be selected from a group, but not limited to, a soy flour, polyvinyl acetates, polyvinyl alcohols, hydroxypropyl methyl cellulose, polysaccharides, proteins, polyethylene glycol, polyvinyl pyrrolidones, styrene acrylic polymer and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of binder, including known, related, and later developed technologies.
In another embodiment, the anti-foaming agent used in the composition mixture for soil drenching may be selected from a group, but not limited to, a polyalkyne oxide modified heptamethyltrisiloxane, 4-(5-dodecyl) benzenesulfonate, docusate (dioctyl sodium sulfosuccinate), benzalkaonium chloride, perfluorooctanesulfonate, naphthalene sulfonic acid, formaldehyde condensate, sodium, polypropylene polyoxyethylene and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of anti-foaming agent, including known, related, and later developed technologies.
In an embodiment, the thickener used in the compositionf or soil drenching may be selected from a group, but not limited to, a guar gum, locust bean gum, carrageenan, alginates, methyl cellulose, sodium carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC). Other types of anti-precipitating agents are based on modified starches, polyacrylates, polyvinyl alcohol and polyethylene oxide, xanthan gum, and a mixture thereof. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of thickener, including known, related, and later developed technologies.
In another embodiment, the suspension agent used in the compositionfor soil drenching may be selected from a group, but not limited to, a sodium carboxymethylcellulose, polyvinylpyrrolidone, polysaccharides, clays, diatomaceous earth powder, and a mixture thereof. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of suspension agent, including known, related, and later developed technologies.
In an embodiment, the wetting agent used in the compositionfor soil drenching may be selected from a group, but not limited to, a sodium lauryl sulfate, sodium dioctyl sulfosuccinate, alkyl phenol ethoxylates and aliphatic alcohol ethoxylates and a mixture thereof. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of wetting agent, including known, related, and later developed technologies.
In another embodiment, the dispersing agent used in the compositionfor soil drenching may be selected from a group, but not limited to, a sodium lignosulfonate, sodium naphthalenesulfonate, formaldehyde, Tristyrylphenolethoxylate phosphate esters, aliphatic alcohol ethoxylates, alkylethoxylates, EO-PO block copolymers and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of dispersing agent, including known, related, and later developed technologies.
In an embodiment, the anti-foaming agent used in the composition for soil drenching may be selected from a group, but not limited to, a silicone and non- silicone.
In another embodiment, the surfactants used in the composition for soil drenching may be selected from a group, but not limited to, an alkyl ethoxylates, linear aliphatic alcohol ethoxylates, aliphatic amine ethoxylates, sorbitan monooleates, sorbitan monooleate ethoxylates, methyl oleate esters and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of surfactants, including known, related, and later developed technologies.
In an embodiment, the non-silicone used in the composition for soil drenching may be selected from a group, but not limited to, an octanol, nonanol, silicon dioxide and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of anti- foaming agent, including known, related, and later developed technologies.
In an embodiment, the silicones used in the composition for soil drenching are selected from a group comprising silicone oils, caron black, alumina oil, colloidal silica, aqueous dimethylpolysiloxane emulsions and a mixture thereof.
In another embodiment, the composition for soil drenching further includes a preservative selected from a group, but not limited to, a propionic acid and its sodium salt, sorbic acid and its sodium or potassium salts, benzoic acid, and its sodium salt, p-hydroxybenzoic acid sodium salt, methyl p-hydroxybenzoate, 1,2 -benzisothiazalin-3-one (BIT) and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of preservative, including known, related, and later developed technologies.
In an embodiment, the composition for soil drenching may further include a plant growth hormone selected from a group, but not limited to, an auxin, gibberellin, cytokinin, abscisic acid or ethylene. In some respects, the auxin may be selected from a group, but not limited to, a naphthalene acetic acid, 2,4 dichlorophenoxyacetic acid, Indole-3-acetic acid (IAA), Indole butyric acid (IBA) and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of hormone, including known, related, and later developed technologies.
In another embodiment, the composition for soil drenching may be in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray and the like. In some aspects of the present disclosure, the multienzyme composition may be in form of an oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable concentrate, nano emulsions, water dispersible granules, water soluble powder, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion or wettable powder, or an encapsulated granule. Embodiments of the present disclosure are intended to include and/or otherwise cover any form of the enzymatic composition, including known, related, and later developed technologies.
In an embodiment, the composition for soil drenching is provided for drenching mix is prepared by mixing cellulase in a range of 2% to 15% w/w of the composition with lipase in a range of 2% to 25% w/w of the composition. Correspondingly the Beta Glucanase in a range of 2% to 25% w/w of the composition, xylanase in a range of 2% to 25% w/w of the composition, protease in a range of 2% to 25% w/w of the composition, laccase in a range of 2% to 35% w/w of the composition, and phytase in a range of 2% to 35% w/w of the composition is added. Further, for liquid formulations.
In another embodiment, the composition is applied to soil after sowing of crops that are grown at a temperature range of 10 °C to 55°C.
In an embodiment, the composition for drenching is capable of increasing soil fertility.
In another embodiment, the composition for soil drenching is provided for enhancing seed germination.
In an embodiment, the composition for soil drenching is provided for promoting seed germination.
In another embodiment, the composition for soil drenching is applied to soil, plant, seeds, seedlings, or roots.
In an embodiment, the composition for soil drenching is used for increasing crop yield.
In another embodiment, the crop may be selected from a group, but not limited to, a cereal, pulses, fruit, and vegetable crops and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of the crop, including known, related, and later developed technologies.
In an embodiment, the composition for soil drenching provides better tolerance against pest.
In another embodiment, the agriculture pests may be selected from a group, but not limited to, soil borne diseases and pathogens, that may include nematodes, mites, aphids, whiteflies, thrips, diamondback moth (Plutella xylostella), and the like. Embodiments of the present disclosure are intended to include and/or otherwise cover any type of the agriculture pest, including known, related, and later developed technologies.
In an embodiment, the composition for soil drenching may contain one or more multienzymes in combinations but not limited to: phytases, any phosphohydrolases, cellulases, any proteolytic multienzyme, glucanases, endo glycosidases, multicopper oxidases, lipases, endo/exo amylases, ACC Deaminases, Urease, Sulfatases, expansins, harpins etc.
In another embodiment, the composition for soil drenching may contain the enzymes that are produced from wild type microbes or from recombinant microbes. Additionally, phytase, lipase, and xylanase production can be achieved using a recombinant yeast expression system, while other enzymes can be produced using wild type filamentous fungus.
In an embodiment, the composition for soil drenching may contain multienzyme mix in unique combination with multiple ingredients but not limited to humic substances, chitosan, seaweed extracts, protein hydrolysates, flours, betaine etc.
In another embodiment, the composition for soil drenching may be applicable for seed coatings, soil spray, foliar spray, drenching, broadcasting, improvement in root absorption etc.
In an embodiment, the composition for soil drenching may improve the soil texture, solubilization of various soil organic/ inorganic components releasing phosphorus, nitrogen, sugars, macro and micronutrients, trace elements in soil, may balance absorption of nitrogen, phosphorus, and potassium, enhance crop stress resistance, cumulatively result in enhanced yield and quality of crops.
In an embodiment, a method of preparing composition for soil drenching, wherein the method comprises steps: (i) weighing enzyme powders, mixing thoroughly in suitable mixer machines; (ii) preparing multienzyme mixture comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase; (iii)adding suitable agriculturally accepted excipients selected from a group comprising plant growth hormones, preservatives, anti-foaming agents, surfactants, dispersing agents, wetting agents, suspension agents, thickeners, binders, emulsifiers, and the like; and (iv) stabilizing the pH of the mixture to a range of 4.5 to 8.
In another embodiment, the prepared composition for soil drenching is in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray, oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable, concentrate, nano emulsions, water dispersible granules, water soluble powder, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion, wettable powder, an encapsulated granule and the like.
In an embodiment, the method of preparing composition for soil drenching includes applying the mixture to soil, plant, crops, leaf, seeds, seedlings, or roots growing at a temperature range of 10 °C to 55°C.
In another embodiment, the agriculturally accepted excipients comprise malt dextrose and enzyme stabilizer.
In an embodiment, the individual enzymes powers are weighed and mixed thoroughly in ribbon mixer.
In another embodiment, the individual enzymes powers are weighed and mixed thoroughly in suitable mixer machines.
In an embodiment, application of the enzyme mixture to the soil has increased its nutritional value and improved the uptake of nutrients by both soil microbes and plants, resulting in higher quality and higher yield crop production. The enzyme mix also has the potential to enhance soil texture, solubilize organic and inorganic components, release essential nutrients like phosphorus, nitrogen, sugars, macro and micronutrients, and trace elements, balance the absorption of nitrogen, phosphorus, and potassium, improve crop stress resistance, and ultimately lead to improved or enhanced crop yield and quality.
In another embodiment, the multienzyme mixture comprising 99 – 99.9 w/w of multienzymes further comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase, along with suitably added agriculturally added excipients show improved activity.
Working Examples
The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices, and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
Examples:
1. Preparation of Composition -Multienzyme soil Drenching Mix
The composition was prepared for the evaluation of parameters. The general composition of the enzymatic composition is described in Table 1 below.
Table 1
Ingredients/Enzymes Exact % ratio by weight Working % ranges
Cellulase 6.000% 2% to 15%
Lipase 15.000% 2% to 25%
Beta Glucanase 17.600% 2% to 25%
Xylanase 11.600% 2% to 25%
Protease 11.800% 2% to 25%
Laccase 29.100% 2% to 35%
Phytase 8.900% 2% to 35%
Agriculturally accepted excipients 0.1 – 1 % 0.1 – 1 %

2. Effect of Composition on Wheat Crop
The effect of Composition was performed to check in India. The seeds taken per acre of the land are represented as Seed Rate/acre and it is 40 Kg/acre. Further, the crop is wheat crop variety is PBW 550. They are commercially available. The trial design is ‘Randomized Block Design in triplicate’. The method of application of the formulation includes spraying over the soil surface just after sowing and before closing the rows with soil. The outcome is presented in two data sets. The first data set compares the outcome between untreated seeds (UT), seeds treated with multienzyme mixture for drenching formulation 1 (F1). The results are compared with the number of days after sowing (DAS) for 15-90 days.
Data set 1: Data set 1 presents the outcome on Seed germination, Crop vigour, Plant height increase, increase in Root weight, increase in shoot weight, increase in leaf length, and increase in leaf width in percentages as presented in below Table 2.
Data Set 1: Table 2
Product % Seed germination (15 DAS) Crop vigour % (70 DAS) Plant height increase %, cm (40 DAS) % increase in Root weight, gm (40 DAS) % increase in shoot weight, gm (40 DAS) % increase in leaf length, cm (90 DAS) % increase in leaf width, cm (90 DAS)

UT 58.4 100 100 100 100 100 100
F1 64.7 120 112 127 122 116 106

Data set 2: Data set 2 presents the outcome on Tillers count for (50DAS), Productive tillers count (90DAS), Panicle length (cm), Panicle weight (gm), Grain count/ panicle, and Yield (Kg/plot) as presented in below Table 3.
Data Set 2: Table 3
Product Tillers count (50DAS) Productive tillers count (90 DAS) Panicle length (cm) Panicle weight (gm) Grain count/ panicle Yield (Kg/plot)
UT 174 87 9.3 2.3 45 5.77
F1 204 97 9.43 2.49 50 6.46

Inference- The tillers count, productive tillers count, panicle length, panicle weight, grain count and yield were increased on applying the multienzymes mixture. The wheat open field trial study showed the beneficial effects of the product on the early germination of seeds and germination rate. Enhanced crop vigor was noted in plants treated with multiple enzymes compared to those left untreated. Moreover, there was a noticeable increase in crop height. Treated crops exhibited a greater number of tillers and improved counts of productive tillers. Additionally, a marked enhancement in panicle length, weight, and grain count was observed, leading to overall higher yields.
3. Effect of Composition on Corn Crop
The effect of Composition was performed to check in India. The seeds taken per acre of the land are represented as Seed Rate/acre and it is 10 Kg/acre. Further, the crop is wheat crop varieties are 4212 (Rasi), Bisco-X-92 (Limagrain), S6668 (Syngenta) and PAC 751 (Advanta). They are commercially available. The trial design is ‘Randomized Block Design in triplicate’. The method of application of the formulation includes spraying over the soil surface just after sowing and before closing the rows with soil. Further, the enzyme mixture is also sprayed over ridges just after sowing. The outcome is presented in two data sets. The first data set compares the outcome between untreated (UT), soil treated with multienzyme mixture for drenching formulation 1 (F1). The results are compared with the number of days after sowing (DAS) for 25-50 days.
Data set 1: Data set 1 presents the outcome on Crop vigour, Plant height increase, increase in stem girth, increase in root weight, and increase in shoot weight in percentages as presented in below Table 4.
Data set 1: Table 4
Product % increase in Crop vigour
(49 DAS) % increase in plant height in cm
(25 DAS) % increase in stem girth in mm
(50 DAS) % increase in root weight in gm
(25 DAS) % increase in shoot weight in gm
(25 DAS)

UT 100% 100 100 100 100
F1 116% 114 115 137 128

Inference: It was apparent that the enhancements were leading to significantly improved yields, particularly when considering high quantities.

Data set 2: Data set 2 presents the outcome on Cobs/plot, increase in test weight of 100 grains, increase in kernels count/cob, and increase in yield in percentages as presented in below Table 5.
Data set 2: Table 5
Product % increase Cobs/plot (80 DAS) % increase in test weight of 100 grains (gm) % increase in kernels count/cob % increase in yield (Kg/plot)
UT 100 100 100 100
F1 113 119 122 131

Inference: It was apparent that the enhancements were leading to significantly improved yields, particularly when considering high quantities.
4. Effect of Composition on Paddy Crop
The effect of Composition was performed to check in India. The seeds taken per acre of the land are represented as Seed Rate/acre and it is 4 to 6 Kg for nursery generation for one acre transplantation. Further, the crop is wheat crop varieties are Arize 8433 (Bayer), PR-126 (PAU) & BPT 5204 (Kaveri seeds). They are commercially available. The trial design is ‘Randomized Block Design in triplicate’. The method of application of the formulation includes broadcasting just after transplantation. The outcome is presented in two data sets. The first data set compares the outcome between untreated seeds (UT), seeds treated with multienzyme mixture for drenching formulation 1 (F1). Figure 1 illustrates Effect of F1 on greenness of leaves using Leaf Color Chart (LCC) for Paddy crop. Figure 2 illustrates Effect of F1 on shoot and root length of Paddy. Further, greenness and effect of F1 on shoot and root length marks the enhanced efficiency of the enzyme mixture. The results are compared with the number of days after sowing (DAT) for 50-90 days.
Data set 1: Data set 1 presents the outcome on Crop vigour, root length increase, increase in shoot length, increase in shoot weight, and increase in root weight in percentages as presented in below Table 6.
Data set 1: Table 6
Product % increase in Crop vigour
(60 DAT) % increase in root length, cm
(55 DAT) % increase in shoot length, cm
(55 DAT) % increase in shoot weight, gm
(55 DAT) % increase in root weight, gm
(55 DAT)

UT 100 100 100 100 100
F1 119 133 114 122 146

Data set 2: Data set 2 presents the outcome on increase in tillers count, increase in productive tillers count, increase in panicle length, increase in panicle weight, increase in grain test weigh, and increase in yield in percentages as presented in below Table 7.
Data set 2: Table 7
Product % increase in tillers count
(50 DAT) % increase in productive tillers count
(90 DAT) % increase in panicle length (cm) % increase in panicle weight % increase in grain test weigh, gm
(1000/plot) % increase in yield (Quintal/acre)

UT 100 100 100 100 100 100
F1 128 131 111 122 109 130

Inference: It was apparent that the enhancements were leading to significantly improved yields, particularly when considering high quantities.
5. Evaluation of the functionality and synergistic effects of active enzymes used for Soil Drenching
The evaluation of enzymes utilized in the formulation (F1) was to assess their activities. To assess the effectiveness and contribution of each enzyme in an enzyme blend formulation (F1), the enzymatic activities of enzymes were examined. An evident synergistic impact was noted among the enzymes present in the formulation (F1). The targeted enzymatic activities of individual enzymes in the final formulated products were effectively achieved. The identification of units of enzyme activities clearly indicates the stability and effectiveness of the multienzyme formulation F1. This data on activity also suggests the absence of inhibitory effects from non-enzymatic components in the formulations, thus confirming an optimal formulation, as also described in below Table 8.
Table 8: Study of Enzyme activities in formulation.
S. No. Name of the
enzyme Enzymatic activities in formulated product
(U/g)
1 Cellulase 11,170
2 Lipase 5,938
3 ß-glucanase 682
4 Xylanase 8150
5 Protease 6,188
6 Laccase 28
7 Phytase 1,582

Advantages of Composition For Soil Drenching:
The Composition for soil drenching is:
• 100% eco-friendly and biodegradable.
• functional at low dosage rates.
• promotes early and uniform seed germination
• improves root and shoot development, higher chlorophyll content, larger leaf blades, higher tiller count, more productive panicle, improved girth measurement, increased nutritional value etc.
• improves crop yields by approximately 20%.
• provides improved biotic and abiotic stress tolerance.
• provides improved soil health & bioremediation.
• assists in the growth of soil microbial flora.
• functionally active at a broad pH range of 4.5 to 8 and temperature range of 10 °C to 55°C.
• application flexibility via broadcasting, drenching, foliar spray on soil.
• starts working immediately on soil or seed without a lag.
The foregoing discussion of the present disclosure has been presented for purposes of illustration and description. It is not intended to limit the present disclosure to the form or forms disclosed herein. In the foregoing Detailed Description, for example, various features of the present disclosure are grouped together in one or more aspects, configurations, or aspects for the purpose of streamlining the disclosure. The features of the aspects, configurations, or aspects may be combined in alternate aspects, configurations, or aspects other than those discussed above. This method of disclosure is not to be interpreted as reflecting an intention the present disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed aspect, configuration, or aspect. Thus, the following claims are hereby incorporated into this Detailed Description, with each claim standing on its own as a separate aspect of the present disclosure.
Moreover, though the description of the present disclosure has included description of one or more aspects, configurations, or aspects and certain variations and modifications, other variations, combinations, and modifications are within the scope of the present disclosure, e.g., as may be within the skill and knowledge of those in the art, after understanding the present disclosure. It is intended to obtain rights which include alternative aspects, configurations, or aspects to the extent permitted, including alternate, interchangeable and/or equivalent structures, functions, ranges or steps to those disclosed, whether or not such alternate, interchangeable and/or equivalent structures, functions, ranges or steps are disclosed herein, and without intending to publicly dedicate any patentable subject matter. ,CLAIMS:1. A composition for soil drenching, comprising:
a) a multienzyme mix with 99 – 99.9 % w/w of multienzymes further comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase; and
b) 0.1 - 1% of agriculturally accepted excipients.

2. The composition as claimed in claim 1, wherein the composition is in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray, oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable, concentrate, nano emulsions, water dispersible granules, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion, wettable powder, soluble powder, an encapsulated granule and the like.

3. The composition as claimed in claim 1, wherein the multienzyme mix are selected from animals, plants, insects, microbes or biological extracts.

4. The composition as claimed in claim 1, wherein the agriculturally accepted excipients comprise malt dextrose and enzyme stabilizer.

5. The multienzyme mixture for drenching as claimed in claim 1, wherein the composition applied to soil after sowing of crops that are grown at a temperature range of 10 °C to 55°C.

6. A method of preparing composition for soil drenching, wherein the method comprising:
i. weighing enzyme powders, mixing thoroughly in suitable mixer machines;
ii. preparing multienzyme mixture comprising: 2% to 15% w/w of cellulase; 2% to 25% w/w of lipase; 2% to 25% w/w of beta glucanase; 2% to 25% w/w of xylanase; 2% to 25% w/w of protease; 2% to 35% w/w of laccase; 2% to 35% w/w of phytase;
iii. adding suitable agriculturally accepted excipients selected from a group comprising plant growth hormones, preservatives, anti-foaming agents, surfactants, dispersing agents, wetting agents, suspension agents, thickeners, binders, emulsifiers, and the like; and
iv. stabilizing the pH of the mixture to a range of 4.5 to 8 to obtain the composition for soil drenching.

7. The method as claimed in claim 6, wherein the obtained composition is in form of an emulsion, microemulsion, granules, powder, suspension, capsule, liquid, spray, oil-in-water emulsion, microemulsion, water-in-oil emulsion, emulsifiable, concentrate, nano emulsions, water dispersible granules, suspension concentrate, capsule suspensions, emulsifiable granule, driftless formulation, oil dispersion, wettable powder, soluble powder, an encapsulated granule and the like.

8. The method as claimed in claim 6, wherein the composition is applied to soil after sowing of crops that are grown at a temperature range of 10 °C to 55°C.