Description:SYNERGISTIC MITICIDAL COMPOSITION WITH (C8-C16) ALKYL AMIDE AND IMPROVED EFFICACY

CROSS-REFERENCE TO RELATED APPLICATION
The present patent application is a patent of addition of the main Indian Patent Application No. 201711046956, filed on December 28, 2017. The present application comprises an improvement or a modification of the invention claimed in the specification of the main patent applied for in Indian Patent Application No. 201711046956.

FIELD OF THE INVENTION
The present invention relates to a stable synergistic composition. More particularly, the present invention relates to a suspo-emulsion composition comprising at least one (C8-C16) alkyl amide as organic solvent in an amount of 15 to 22% by weight.

BACKGROUND OF THE INVENTION
Agriculture pests, such as mites, have long been a major threat to worldwide food production and agricultural productivity. Mites can cause significant harm to a wide variety of crops, including fruits, vegetables, and ornamental plants, by feeding on plant tissues and causing stress, reduced growth, and crop failure. Furthermore, the mites multiply quickly and develop resistance, making it difficult to control mite populations in agriculture. The adoption of high-yielding crop types, improved agricultural practices, and excessive irrigation may all contribute to rapid mite growth. Mites are responsible for a considerable loss of yield in agriculture, causing an estimated 34% yield loss. To mitigate these challenges, efficient miticidal composition capable of mite population control must be developed.

There are numerous formulations available to control mites; however, they are ineffective and often result in resistance development. IN284/MUM/2014 describes a pyriproxyfen (with Poly Aryl Phenol or Octyl Phenol Ethoxylates) and diafenthiuron (Magnesium Aluminium Silicate and Acrylic polymer) SE formulation.

Ensure appropriate dispersions and actives stability is one of the most difficult problems in developing synergistic suspo-emulsion compositions. Furthermore, none of the prior art disclose a stable, synergistic suspo-emulsion composition comprising (C8-C16) alkyl amide for effective mite control. Hence, a stable suspo-emulsion composition that not only delays the development of resistance but also has translaminar properties, a synergistic effect, and stability is required.

OBJECTIVE OF THE PRESENT INVENTION
It is primary objective of the present invention is to provide a stable, synergistic suspo-emulsion composition comprising of at least one (C8-C16) alkyl amide.

It is another objective of the present invention is to provide a stable, synergistic suspo-emulsion composition comprising at least one (C8-C16) alkyl amide in the range of 15 to 22% by weight.

It is another objective of the present invention is to provide a process for preparation of the stable, synergistic suspo-emulsion composition.

SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts in a simplified form that are further described below in the Detailed Description. This summary is not intended to identify key features or essential features of the claimed subject matter. Nor is this summary intended to be used to limit the claimed subject matter’s scope.

Both the foregoing summary and the following detailed description provide examples and are explanatory only. Accordingly, the foregoing summary and the following detailed description should not be considered to be restrictive. Further, features or variations may be provided in addition to those set forth herein. For example, embodiments may be directed to various feature combinations and sub-combinations described in the detailed description.

Accordingly, present invention aims to provide a suspo-emulsion composition comprising:
a) pyriproxyfen;
b) diafenthiuron;
c) at least one (C8-C16)alkyl amide as an organic solvent; and
d) agriculturally acceptable adjuvants,
wherein the (C8-C16)alkyl amide is in the range of 15 to 22% w/w.

In another aspect of the invention, the pyriproxyfen is in the range of 8 to 8.6 % w/w.

In another aspect of the invention, the diafenthiuron is in the range of 29.5 to 31% w/w.

In yet another aspect of the present invention, the (C8-C16) alkyl amide organic solvent is selected from the group comprising of N, N- Dimethyl decanamide, N, N-Dimethyl octanamide or a blend thereof.

In another aspect of the invention, the viscosity of the suspo-emulsion composition is in range of 350-550 cps.

In further aspect of the present invention, the agriculturally acceptable adjuvants is selected from the group of anti-foaming agents, anti-freezing agents, dispersing agents, emulsifiers, thickeners, or a mixture thereof.

In further aspect of the present invention, the anti-foaming agent is selected from silicon defoamer, Polydimethylsilioxane, Polymethylhydrosiloxane, cyclomethicones or a mixture thereof and is in the range of 0.25% to 0.5% by weight of the total weight of the composition.

In another aspect of the present invention, the emulsifier is selected from the group comprising calcium dodecylbenzene sulphonate, ethoxylated polyarylphenol in solvent petroleum naphtha, benzenesulphonic acid derivatives calcium salt, sodium dioctyl sulfosuccinate, thiophosphoro succinate salt, taurine derivatives (in particular alkyl taurate), ethoxylated fatty glyceride or a mixture thereof and is in the range of 3% to 12% by weight of the total weight of the composition.

In another aspect of the present invention, the dispersing agent is in the range of 0.5% to 3% by weight of the total weight of the composition, and the anti-freezing agent is in the range of 7% to 10% by weight of the total weight of the composition.

DETAILED DESCRIPTION OF THE INVENTION
Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods. The illustrative examples are described in this section in connection with the embodiments and methods provided. The invention according to its various aspects is particularly pointed out and distinctly claimed in the appended claims read in view of this specification and appropriate equivalents.

It is to be noted that, as used in the specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.

The expression of various quantities in terms of “% w/w” or “%” means the percentage by weight, relative to the weight of the total solution or composition unless otherwise specified.

The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control of insects-pests or disease.

The term formulation and composition as used herein conveys the same meaning and can be used interchangeably.

“Bioactive amounts” as mentioned herein means that amount which, when applied treatment of crops, is sufficient to effect such treatment. The bioactive amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like.

The term “synergistic”, as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.

The term “control” means to inhibit the ability of pests to survive, grow, feed and/or reproduce, or to limit the pest related damage or loss in crop plants.

To “control” pests may or may not mean killing the insects although, it preferably means killing the pests.

The present invention aims to provide a stable, synergistic suspo-emulsion composition comprising at least one (C8-C16) alkyl amide in the range of 15 to 22% by weight, diafenthiuron and pyriproxyfen and other agrochemically acceptable adjuvants.

The stable synergistic suspo-emulsion composition comprises of:
a) pyriproxyfen;
b) diafenthiuron;
c) at least one (C8-C16)alkyl amide; and
d) agriculturally acceptable adjuvants.

In an embodiment of the present invention, the agriculturally acceptable adjuvants are selected from the group of anti-foaming agents, anti-freezing agents, dispersing agents, emulsifiers, xanthum gum, or a mixture thereof.

Further, the stable, synergistic miticidal composition is a suspo-emulsion composition that comprises diafenthiuron, pyrioxyfen, an emulsifier, anti-freezing agent, a dispersing agent, an antifoaming agent, suspending agent, and thickeners.

The concentration of pyriproxyfen in the suspo-emulsion composition is from 8% to 8.6% by weight of the total weight of the composition.

The concentration of diafenthiuron in the suspo-emulsion composition is from 29.5% to 31% by weight of the total weight of the composition.

The concentration of (C8-C16) alkyl amide in the suspo-emulsion is from 15% to 22% by weight of the total weight of the composition. Preferably, in the range of 18% to 21% by weight of the total weight of the composition.

Surprisingly, the insecticidal and/or miticidal activity of the active compound combinations according to the invention was found to be significantly higher than the sum of the activities of the individual active compounds. It has surprisingly been found that the combination of a pyriproxyfen with diafenthiuron and (C8-C16) alkyl amide leads to a surprising, unexpected, and synergistic enhancement in the efficacy of the combination.

The suspo-emulsion composition of the present invention is used to control mites of the family Tetranychidae (Spider Mite, Sugarcane Red Spider Mite, Fig spider mite, Oriental red mite, Citrus brown mite, Red tea mite, Mango red spider mite, Mango red spider mite, European red mite, Brown wheat mite, White patchy, sugarcane leaf spotted mite, web mite, Red-legged spider mite, Bean spider mite, Red vegetable mite, Two spotted spider mite), Tenuipalpidae/False Mites (Citrus flat mite, Scarlet mite, Reddish black flat mite, Eriophyidae (Leprosis mite, Pineapple flat mite, Bud Gall mite, Pigeon pea sterility mosaic mite, Coconut mite, Litchi erineum mite, Brinjal erineum mite, Tomato erineum mite, Mango bud mite, Jujube gall mite, and Tarsonemidae (Citrus silver mite, broad mite, Rice sheath mite, Dahlia mite, yellow tea mite, Pink mite, Purple tea mite.

The suspo-emulsion composition of the present invention is preferably used for the control of mites in Wheat, Barley, Oats, Maize, Sorghum, Sugarcane, Areca catechu (Palm), Fig, Apple, Citrus, Cotton, Cucurbits, Coffee, Mango, Tea, Coriander, Beans, Vegetables, Cucurbits, Ornamental Plants, Bean, Brinjal, Okra, Guava, Papaya, Pineapple, Pigeon pea, Coconut, Litchi, Chilli, Jute, Potato, Rice, Almond, Castor, Jujube, Red Date, Golden Rain Tree, Lemon, Litchi, Golden Shower Tree, Gourds, Sweet Potato, Garlic, Onion, Marigold, Jasmine, Chrysanthemums, Areca, Coconut palms.

In a preferred embodiment of the present invention, the stable composition is the suspo-emulsion type. Suspo-emulsion (SE) formulations are used to combine two active ingredients with very different physical properties into one formulation. They are a combination of suspension concentrate (SC) and concentrated aqueous emulsion (EW) technologies. Suspo-emulsion is a formulation containing both solid and liquid active ingredients dispersed in an aqueous phase. An aqueous suspo-emulsion is a mixture of water-insoluble active ingredients dispersed in an aqueous solution, where one (or more) of the active ingredients is in suspension form and one (or more) of the active ingredients is in emulsion form. The formulation is intended for dilution into water prior to spray application.

Further, the effective amount of (C8-C16) alkyl amide as organic solvent in the present invention leads the pesticides to enter the plant leaf after application of the composition, with penetration occurring primarily through the leaf cuticle.

In an embodiment, the stable composition of the present invention comprises (C8-C16) alkyl amide as an organic solvent, which is selected from the group comprising N,N-Dimethyl heptanamide, N,N-diethyl heptanamide, N,N-Dimethyl octanamide, N,N-Diethyl octanamide, N,N-dimethyl nonanamide, N,N-Diethyl nonanamide, N,N-Dimethyl decanamide, N,N-Diethyl decanamide, N,N Dimethyl undecanamide, N,N-Diethyl undecanamide, N,N-Dimethyl dodecanamide, N,N-Diethyl dodecanamide or a mixture/or blend thereof. In accordance with one preferred embodiment of the present invention, the solvent used are N, N-Dimethyl decanamide or N, N-Diethyl octanamide or commercially available RHODIASOLV® (a blend of N, N-Dimethyl octanamide and N, N-Dimethyl decanamide) or a mixture thereof.

In a preferred embodiment, the (C8-C16) alkyl amide is from 18% to 21% by weight of the total weight of the composition.

The emulsifier used in the present invention is selected from the group comprising of calcium (linear and branched) alkyl benzene sulfonate in 2-ethyl hexanol solvent, calcium salts of alkyl (4C10-14 derivatives) benzene sulfonic acid, sodium dioctyl sulfosuccinate, ethoxylated fatty glyceride, thiophosphoro succinate salt, taurine derivatives (in particular alkyl taurate), commercially available emulsifier having trade name GERONOL FF/6E® (a mixture of calcium dodecylbenzene sulphonate, ethoxylated polyarylphenol and solvent petroleum naphtha) or a mixture thereof. According to one embodiment, the emulsifier used is most preferably in the range of 3% to 12% weight by weight.

The dispersing agent used in the present invention is selected from the group comprising commercially available dispersing agents having the trade name 10 Agnique® BP NP-4030 EO-PO Poly, Agnique® CP 72 L, Agnique® CSO 40-U, Agnique® CSO-20, Agnique® CSO-36 Ethox Castor Oil, Agnique® DDL Dispersant, IG Surf® 2370 SC, IG Surf® 2380. In accordance with a preferred embodiment of the present invention, the preferred ones are Agnique® CP 72 L (Oxirane, 2-methyl-, polymer with oxirane, mono (2-propylheptyl) ether) and IG Surf® 2380 (Polyoxyethylenated Triglycerides, fatty Alcohol Ethoxylate). Dispersing agent used in the composition of the present invention improves the separation of particles and helps to prevent settling or clumping in the composition, which ultimately enhances the stability of the composition.

According to one of the embodiments, dispersing agents are in the range of 0.5% to 3% weight by weight. The anti-freezing agent used in the present invention is selected from a group comprising propylene glycols, polypropylene glycols, polyethylene glycols, ethylene glycols, and glycerin, most preferably in the range of 7% to 10% weight by weight.

Anti-foaming agent to prevent the formation of foam as used in the present invention is selected from the group comprising silicon defoamer, polydimethylsiloxane, polymethylhydrosiloxane, and cyclomethicones. The antifoaming agent may enable the wetting agent and the dispersant, when included, to function properly within the mixture and may facilitate the spraying of insecticide while reducing the chances of the sprayer becoming clogged with foam or entrained air. In accordance with a preferred embodiment of the present invention, the preferred ones are silicon defoamers and polydimethylsiloxane used in the composition, most preferably in the range of 0.25% to 0.5% weight by weight.

Optionally, the present invention comprises suspending agent silica or fumed silica.

In another embodiment, the present invention comprises an anti-microbial agent selected from the group comprising of benzisothiazolinone, methylisothiazolinone, copper pyrithione, and zinc pyrithione, commercially available with trade name PROXEL GXL®, which is a 20% aqueous dipropylene glycol solution of 1,2-benzisothiazolin-3-one.

Further, the composition comprises thickener selected from the group comprising xanthan gum, agar agar, and arabic gum. The problems of long shelf life of agrochemical composition can be minimized through the use of rheology modifiers or additives that promote, for example, the inhibition of sediment in the active ingredients in a given formulation. Such additives increase the viscosity of the agrochemical composition, providing greater stability and physical efficiency and enabling its storage for a longer period. These additives are usually called swelling agents, anti-settling agents, stabilizing agents or thickeners and may be present alone or in the form of a composition.

In another embodiment of the present invention, the composition may optionally comprise a stabilizing agent selected from a group comprising colloidal silicon dioxide, bentonite, and/or mixture thereof, which is used in the present invention in an amount ranging from 0.25% to 3% by weight.
In an embodiment of the invention, the viscosity of the suspo-emulsion composition is in range of 350-550 cps, preferably 380 to 500 cps.

The following examples are intended to illustrate, but in no way limit the scope of, the present invention.

EXAMPLES
Example 1

Preparation Process:
Step 1: Emulsifiable Concentrate (EC)
Required quantity of the pyriproxyfen and (C8-C16) alkyl amide mixed in a vessel to obtain a clear solution. Followed by the addition of an emulsifier to the clear solution and mixing to obtain an emulsifiable concentrate (EC) at 15? to 45?.

Step 2: Suspension Concentrate (SC)
Required quantity of water, dispersing agent, suspending agent, anti-foaming agent, and dispersing agent were mixed and stirred for 10 minutes. Followed by the addition of diafenthiuron slowly at 15? to 45? and bead milling twice to obtain a particle size below 10 microns.

Step 3:
Homogenizing the EC (step 1) and SC (step 2) with a high-speed homogenizer at 1000 RPM for 1 hour Followed by the addition of Xanthum gum and biocide, and optionally stabilizer, and homogenization to obtain the final suspo-emulsion (SE) composition.

Exemplary composition of present invention prepared by above process are provided in example 2 to 5:

Example 2:
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 140.21
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.20
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 180.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water 40.00
Total SE Formulation 1000
Analytical Observation
Description: White Color Homogeneous Liquid
Dispersibility 0 Hrs No Separation, No Sedimentation
Dispersibility 2 Hrs No Separation, No Sedimentation
pH (1%) 6.62
Foaming (1%) 10mL
Viscosity 420cps
Pyriproxyfen AI 8.06% w/w
Diafenthiuron AI 30.07% w/w

Example 3:
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 140.21
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.14
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 150.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water 70.00
Total SE Formulation 1000
Analytical Observation
12. Description: White Color Homogeneous Liquid
13. Dispersibility 0 Hrs No Separation, No Sedimentation
14. Dispersibility 2 Hrs No Separation, No Sedimentation
15. pH (1%) 6.75
16. Foaming (1%) 12 mL
17. Viscosity 420cps
18. Pyriproxyfen AI 8.06% w/w
19. Diafenthiuron AI 30.02 % w/w

Example 4:
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 140.21
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.14
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 220.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water -
Total SE Formulation 1000
Analytical Observation
12. Description: White Color Homogeneous Liquid
13. Dispersibility 0 Hrs No Separation, No Sedimentation
14. Dispersibility 2 Hrs No Separation, No Sedimentation
15. pH (1%) 6.82
16. Foaming (1%) 10 mL
17. Viscosity 450cps
18. Pyriproxyfen AI 8.06% w/w
19. Diafenthiuron AI 30.02 % w/w

Example 5:
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 140.21
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.14
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 200.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water 20.00
Total SE Formulation 1000
Analytical Observation
12. Description: White Color Homogeneous Liquid
13. Dispersibility 0 Hrs No Separation, No Sedimentation
14. Dispersibility 2 Hrs No Separation, No Sedimentation
15. pH (1%) 6.76
16. Foaming (1%) 12 mL
17. Viscosity 450cps
18. Pyriproxyfen AI 8.06% w/w
19. Diafenthiuron AI 30.02 % w/w

Example 6:
Methodology
The experiment was conducted as per the randomized block design with 3 replications and 8 treatments in a single plot area of 20 m2. The total area of the experimental plot was 500 m2. 15 plants were randomly selected and tagged per treatment per replication. Prior to the application, a test sample as per the treatment schedule was applied at pest appearance by using 200 litres of water per acre using a knapsack sprayer with a hollow cone nozzle.
Crop Brinjal
Variety Pusa Purple Long
Target Pest Mites
Date of observations 3 DAA and 7DAA

Table 1:

S. No Treatment Dose/HA Pre-count Mite Population per leaf
3 DAA 7DAA
Average % Control Mean % Control
1. Pyriproxyfen 8% + Diafenthiuron 30% SE + 14% Solvent 1000 1.07 0.78 43.07 0.29 77.52
2. Pyriproxyfen 8% + Diafenthiuron 30% SE + 15% Solvent 1000 1.13 0.79 45.18 0.27 79.66
3. Pyriproxyfen 8% + Diafenthiuron 30% SE + 18% Solvent 1000 1.16 0.80 45.92 0.25 81.90
4. Pyriproxyfen 8% + Diafenthiuron 30% SE + 20% Solvent 1000 1.07 0.73 46.50 0.23 82.01
5. Pyriproxyfen 8%+ Diafenthiuron 30% SE + 22% solvent 1000 1.20 0.77 49.53 0.24 82.98
6. Pyriproxyfen 10%EC 500 1.16 1.43 3.36 1.31 5.78
7. Diafenthiuron 50% WP 750 1.09 1.01 27.27 0.49 62.48
8. Control 1.28 1.62 0.00 1.53 0.00

Procedure:
• Pre-counts were taken prior to the spray conduction with the help of a 10x magnifying lens.
• Pest’s count (area of observation): selected 15 plants per treatment and counted number of live adults per plant.
• After the spray, the population count was taken at 3 days and 7 days after application.

Table 1 shows the population of Brinjal mites recorded in different pesticide treatments. The data showed that there were no significant changes in the population of Brinjal mites before spraying, indicating that the pest was distributed evenly across the experimental plots. The results collected from the first spray demonstrated a considerable reduction in the population of Brinjal mites in all treated plots compared to the untreated control. Brinjal mite population reduction was much higher in T5: Pyriproxyfen 8% + Diafenthiuron 30% SE + 22% solvent (49.53% and 82.98 at 3 DAA and 7 DAA). T4: Pyriproxyfen 8% + Diafenthiuron 30% SE + 20% Solvent yielded 46.50% and 82.01%, respectively, with 3 DAA and 7 DAA. However, these insecticides greatly reduced the incidence of Brinjal mites compared to the untreated control (0.00%).

Example 7:
Crop Chilli
Variety Pusa Jwala
Target Pest Mites
Date of observations 3 DAA and 7DAA

Methodology
The experiment was conducted as per the randomized block design with 3 replications and 8 treatments in a single plot area of 20 m2. The total area of the experimental plot was 500 m2. 15 plants were randomly selected and tagged per treatment per replication. Prior to the application, a test sample as per the treatment schedule was applied at pest appearance by using 200 litres of water per acre using a knapsack sprayer with a hollow cone nozzle.

Table 2
S. No. Treatment Dose/HA Pre-count 3DAA 7DAA
Mean % Control Mean % Control
1. Pyriproxyfen 8% + Diafenthiuron 30% SE + 14% Solvent 1000 1.06 0.94 35.00 0.27 79.93
2. Pyriproxyfen 8% + Diafenthiuron 30% SE + 15% Solvent 1000 1.10 0.76 49.90 0.26 81.78
3. Pyriproxyfen 8% + Diafenthiuron 30% SE + 18% Solvent 1000 1.15 0.79 49.95 0.23 84.48
4. Pyriproxyfen 8% + Diafenthiuron 30% SE + 20% Solvent 1000 1.12 0.76 50.08 0.21 85.61
5. Pyriproxyfen 8%+ Diafenthiuron 30% SE + 22% solvent 1000 1.19 0.76 53.18 0.22 85.54
6. Pyriproxyfen 10%EC 500 1.17 1.39 13.38 1.29 14.56
7. Diafenthiuron 50% WP 600 1.14 1.02 34.42 0.47 67.76
8. Control Nill 1.15 1.57 0.00 1.48 0.00

Observation:
• Pre-counts were taken prior to the spray conduction with the help of a 10x magnifying lens.
• Pest’s count (area of observation): selected 15 plants per treatment and counted number of live adults per plant.
• After the spray, a population count was taken 7 days after application.

Table 2 shows the population of chilli mites recorded in different pesticide treatments. The data showed that there were no significant differences in the number of Chilli mites before spraying, indicating that the pest was distributed evenly across the experimental plots. The data collected from the first spray demonstrated a considerable reduction in the population of Chilli mites in all treated plots compared to the untreated control. Chilli mite population reduction was much higher in Pyriproxyfen 8%+ Diafenthiuron 30% SE+ 22% solvent @ 1000ml/ha (85.54% at 7DAA). At 7DAA, T4: Pyriproxyfen 8%+ Diafenthiuron 30% SE+ 20% solvent @ 1000ml/ha was 85.61%. These pesticides, however, had a much lower frequency of Chilli mites than the untreated control (0.00%).

T4: Pyriproxyfen 8%+ Diafenthiuron 30% SE+ 20% solvent @ 1000 ml/ha showed comparable results to T5: Pyriproxyfen 8%+ Diafenthiuron 30% SE+ 22% solvent @ 1000 ml/ha.

Example 8:
Table 3:
Efficacy trial of formulation on Chilli (Capsicum annum)
Treatment Dose/HA Effect on Mites 7DAA
Observed Expected Ratio Synergy

Pyriproxyfen 8% + Diafenthiuron 30% SE + 14% Solvent 1000 79.93 72.4 1.1 Yes
Pyriproxyfen 8% + Diafenthiuron 30% SE + 15% Solvent 1000 81.78 72.4 1.12 Yes
Pyriproxyfen 8% + Diafenthiuron 30% SE + 18% Solvent 1000 84.48 72.4 1.16 Yes
Pyriproxyfen 8% + Diafenthiuron 30% SE + 20% Solvent 1000 85.61 72.4 1.18 Yes
Pyriproxyfen 8%+ Diafenthiuron 30% SE + 22% solvent 1000 85.54 72.4 1.18 Yes
Pyriproxyfen 10%EC 500 14.56 - - -
Diafenthiuron 50% WP 600 67.76 - - -
Control NA 0.00 - - -
* HA-Hectare **DAA: Days after Application
Example 9:
Table 4: Efficacy trail of formulation on Brinjal
S. No Treatment Dose/HA Effect on Mites 7DAA
Observed Expected Ratio Synergy

1. Pyriproxyfen 8% + Diafenthiuron 30% SE + 14% Solvent 1000 77.52 64.6 1.2 Yes
2. Pyriproxyfen 8% + Diafenthiuron 30% SE + 15% Solvent 1000 79.66 64.6 1.23 Yes
3. Pyriproxyfen 8% + Diafenthiuron 30% SE + 18% Solvent 1000 81.9 64.6 1.26 Yes
4. Pyriproxyfen 8% + Diafenthiuron 30% SE + 20% Solvent 1000 82.01 64.6 1.26 Yes
5. Pyriproxyfen 8%+ Diafenthiuron 30% SE + 22% solvent 1000 82.98 64.6 1.28 Yes
6. Pyriproxyfen 10%EC 500 5.78 - - -
7. Diafenthiuron 50% WP 750 62.48 - - -
8. Control NA 00 - - -
* HA-Hectare **DAA: Days after Application

It is evident from tables 3 and 4 that the composition shows excellent efficacy as compared to solo formulations.

Example 10:
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 100.15
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.20
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 230.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water 30.00
Total SE Formulation 1000
Analytical Observation
12. Description: White Color High Viscous Liquid
13. Dispersibility 0 Hrs No Separation, No Sedimentation
14. Dispersibility 2 Hrs No Separation, No Sedimentation
15. pH (1%) 6.85
16. Foaming (1%) 15 mL
17. Viscosity 590 cps
18. Pyriproxyfen AI 8.05% w/w
19. Diafenthiuron AI 30.08 % w/w

Example 11
S.NO INGREDIENTS QTY (g)
Suspension Concentrate (SC)
1. Water 100.15
2. Silicon defoamer 5.00
3. Propylene Glycol 80.00
4. Agnique CP 72L 20.00
5. Silica 5.00
6. Diafenthiuron technical @98% 307.10
Emulsifiable Concentrate (EC)
7. Pyriproxyfen technical @98% 82.65
8. N,N-Dimethyl decanamide 240.00
9. GERONOL FF/6E 120.00
10. Xanthum Gum @ 2 % Gel with BT-20 20.00
11. Water 20.00
Total SE Formulation 1000
Analytical Observation
12. Description: White Color High Viscous Liquid
13. Dispersibility 0 Hrs No Separation, No Sedimentation
14. Dispersibility 2 Hrs No Separation, No Sedimentation
15. pH (1%) 6.82
16. Foaming (1%) 10 mL
17. Viscosity 600 cps
18. Pyriproxyfen AI 8.06% w/w
19. Diafenthiuron AI 30.02 % w/w

As shown above, formulations with more than 22% organic solvent tend to produce a highly viscous liquid. It is important to note that high viscosity has a negative impact on both the preparation process and the composition's stability. Furthermore, the highly viscous composition necessitates more energy to flow, making it less efficient and non-economical. High viscosity reduces the composition's pourability and flowability. Examples 10 and 11 exhibit viscosities of 590 and 600 cps, respectively, whereas examples 2, 3, 4, and 5, homogeneous liquid, have viscosities in the range of 420 cps and 450 cps when the organic solvent is in the range of 15 to 22%.

While this invention has been described in detail with reference to certain preferred examples, it should be appreciated that the present invention is not limited to those precise examples rather, in view of the present disclosure, which describes the current best mode for practicing the invention, many modifications and variations would present themselves to those skilled in the art without departing from the scope and spirit of this invention. This invention is susceptible to considerable variation in its practice within the spirit and scope of the appended claims.
, Claims:We Claim:
1. A suspo-emulsion composition comprising:
a) pyriproxyfen;
b) diafenthiuron;
c) at least one (C8-C16)alkyl amide as an organic solvent; and
d) agriculturally acceptable adjuvants,
wherein the (C8-C16)alkyl amide is in the range of 15 to 22% w/w.

2. The suspo-emulsion composition as claimed in claim 1, wherein the pyriproxyfen is in the range of 8 to 8.6 % w/w.

3. The suspo-emulsion composition as claimed in claim 1, wherein the diafenthiuron is in the range of 29.5 to 31% w/w.

4. The suspo-emulsion composition as claimed in the claim 1, wherein the (C8-C16)alkyl amide organic solvent is selected from the group comprising of N,N- Dimethyl decanamide, N,N-Dimethyl octanamide or a blend thereof.

5. The suspo-emulsion composition as claimed in claim 1, wherein the agriculturally acceptable adjuvants is selected from the group of anti-foaming agents, anti-freezing agents, dispersing agents, emulsifiers, thickeners, or a mixture thereof.

6. The suspo-emulsion as claimed in claim 1, wherein the anti-foaming agent is selected from silicon defoamer, Polydimethylsilioxane, Polymethylhydrosiloxane, cyclomethicones or a mixture thereof and is in the range of 0.25% to 0.5% by weight of the total weight of the composition.

7. The suspo-emulsion composition as claimed in claim 1, wherein the emulsifier is selected from the group comprising calcium dodecylbenzene sulphonate, ethoxylated polyarylphenol in solvent petroleum naphtha, benzenesulphonic acid derivatives calcium salt, sodium dioctyl sulfosuccinate, thiophosphoro succinate salt, taurine derivatives (in particular alkyl taurate), ethoxylated fatty glyceride or a mixture thereof and is in the range of 3% to 12% by weight of the total weight of the composition.

8. The suspo-emulsion composition as claimed in claim 1, wherein the dispersing agent is in the range of 0.5% to 3% by weight of the total weight of the composition.

9. The suspo-emulsion composition as claimed in claim 1, wherein the anti-freezing agent is in the range of 7% to 10% by weight of the total weight of the composition.