Description:FIELD OF THE INVENTION
[0001] The present invention relates to a mosquito repellent composition. Specifically, the present invention relates to a synergistic herbal mosquito repellent composition comprising a microencapsulated aqueous dispersion of a combination of natural or herbal active ingredients, and a method of preparation thereof. The composition can be applied on textiles, paper and non-woven surfaces for prolonged mosquito repellent property.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Disease spread through mosquitoes is very critical problem that needs to be addressed on a global scale. The composition is always crucial, especially, based on natural ingredients which tend to perform on repellency action rather than mortality action by active ingredients based on synthetic formulas. Selection of the natural oils to create a right composition, make it suitable for the intended application and then test the applied substrate for the results.
[0004] Patent No: CN101420848B describes the application of microencapsulated essential oil but does not describe on the mosquito repellent properties and application thereof of the microencapsulated essential oil on textiles, paper and non-woven.
[0005] Patent No: CA2087364C describes the use of permethrin as a mosquito repellent but has not described the natural essential oils and their combination as an effective mosquito repellent for aqueous application on textiles, paper and non-woven.
[0006] Patent No: EP3065542A1 describes the mosquito repellent composition and its use in textile application but has not described its effect after microencapsulation of the composition for better durability of the textiles that are subjected to washing cycles.
[0007] Patent No: US20200390094A1 describes the composition of combination of natural active ingredients for application on textile substrates but does not describe its effect after the microencapsulation of the composition containing the combination of natural active ingredients.
[0008] The technology of microencapsulation to prepare the microcapsule dispersion is crucial in preparing the aqueous dispersion of the composition of natural active ingredients for application on textiles, paper and non-woven in aqueous processes.
[0009] Many natural active ingredients have been formulated and then microencapsulated to prepare a stable aqueous dispersion which were then applied on textiles, paper and certain non-woven substrates. Further, these were tested under the modified-WHO test protocol specifying the testing conditions in a cage to determine the ideal composition.
[0010] The purpose of the present invention is to offer a unique and high performing composition of natural active ingredients and its application on textile, paper and non-woven as targeted substrates through the technology of microencapsulation to impart mosquito repellent properties.
[0011] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.

OBJECTS OF THE INVENTION
[0012] Objects of the present invention are to provide a mosquito repellent composition comprising natural ingredients.
[0013] An object of the present invention is to provide a synergistic herbal mosquito repellent composition created for its mosquito repellent properties based on performance through sensory perception.
[0014] An object of the present invention is to provide a synergistic herbal mosquito repellent composition which will be effective for a longer time duration by exhibiting sustained release profile, triggered by methods including but not limiting to pressure and mechanical friction.
[0015] An object of the present invention is to provide a synergistic herbal mosquito repellent composition comprising better stability to thermal application conditions, and providing resistance against the environmental conditions like light, ultra-violet radiations, storage temperature conditions, biological factors and the like.
[0016] Another object of the present invention is to provide a synergistic herbal mosquito repellent composition comprising a microencapsulated dispersion of the natural active ingredients in aqueous media.
[0017] Another object of the present invention is to provide a synergistic herbal mosquito repellent composition comprising a microencapsulated dispersion of the natural active ingredients for application on textiles, non-woven and woven surfaces, paper, and the like.
[0018] Yet another object of the present invention is to provide a method of preparing the synergistic herbal mosquito repellent composition comprising a microencapsulated dispersion of the natural active ingredients for application on textiles, non-woven and woven surfaces, paper, and the like.

SUMMARY OF THE INVENTION
[0019] This summary is provided to introduce a selection of concepts in a simplified form that is further described below in the detailed description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
[0020] Aspects of the present invention relates to a mosquito repellent composition. Specifically, the present invention relates to a synergistic herbal mosquito repellent composition comprising a microencapsulated aqueous dispersion of a combination of natural active ingredients, and a method of preparation thereof.
[0021] In an aspect, the present invention relates to a synergistic herbal mosquito repellent composition created for its mosquito repellent properties based on performance through sensory perception.
[0022] In an aspect, the present invention relates to a synergistic herbal mosquito repellent composition which will be effective for a longer time duration by exhibiting sustained release profile, triggered by methods including but not limiting to pressure and mechanical friction.
[0023] In an aspect, the present invention relates to a synergistic herbal mosquito repellent composition comprising better stability to thermal application conditions, and providing resistance against the environmental conditions like light, ultra-violet radiations, storage temperature conditions, biological factors and the like.
[0024] In an aspect, the present invention relates to a synergistic herbal mosquito repellent composition, said composition comprises:
a. a base composition comprising
i. a single or a combination of natural active ingredients,
b. encapsulant,
c. emulsifier,
d. dispersant,
e. foam suppressant, and
f. optionally, one or more additives.
[0025] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition, said composition comprises:
a. a base composition in an amount of 10% to 60% V/V of the whole composition comprising
i. a single or a combination of natural active ingredients having mosquito repellent activity in an amount of 10% to 60% V/V of the base composition, wherein, the natural active ingredients selected from the group consisting of citronella oil, lemon eucalyptus oil, eucalyptus globulus oil, cinnamon bark oil, tea tree oil, soybean oil, geraniol oil, neem seed oil, neem seed oil with high azadirachtin content, and lemon grass oil, and.
b. encapsulant in an amount of 1% to 10% V/V of the whole composition,
c. emulsifier in an amount of 1% to 5% V/V of the whole composition,
d. dispersant in an amount of 0.5% to 5% V/V of the whole composition,
e. foam suppressant in an amount of 0.1% to 1% V/V of the whole composition,
f. optionally, one or more additives, and
g. water to make 100%
[0026] In another aspect, synergistic herbal mosquito repellent composition further comprises polyethylene glycol in an amount of 0.1 to 0.5% V/V, EMA in an amount of 0.1 to 1% V/V, diethylene glycol in an amount of 0.01 to 0.2% V/V, and urea in an amount of 0.01 to 0.1% V/V of the whole composition
[0027] In another aspect, the present invention provides a method for preparing synergistic herbal mosquito repellent composition disclosed herein, said method comprising the steps of:
a. Mixing natural active ingredients under a high-speed agitation of minimum 3000 rpm for 10 minutes to obtain a stable composition of microscopic droplets,
b. Microencapsulating the stable composition within capsule wall of the encapsulant to obtain the base composition,
c. Emulsifying by adding an emulsifier to the base composition to obtain aqueous slurry, and
d. Curing the aqueous slurry at 50 to 90 0C to give the strength to the microcapsule walls, followed by cooling to obtain the synergistic herbal mosquito repellent composition
[0028] In yet another aspect, the synergistic herbal mosquito repellent composition is suitable for deposition textile, woven, or non-woven surfaces for longer duration activity. The synergistic herbal mosquito repellent composition can be applied on said surfaces during processing, finishing, coating, printing, dipping, spraying, and the like.
[0029] Various objects, features, aspects, and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments

BRIEF DESCRIPTION OF THE FIGURES
[0030] FIG. 1A and 1B: Scanning electron microscopic (SEM) images of microcapsules of the synergistic herbal mosquito repellent composition.

DETAILED DESCRIPTION OF THE INVENTION
[0031] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure.
[0032] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0033] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0034] In some embodiments, numbers have been used for quantifying weight percentages, ratios, and so forth, to describe and claim certain embodiments of the invention and are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0035] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0036] As used in the description herein and throughout the claims that follow, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise.
[0037] Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0038] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[0039] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0040] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified.
[0041] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0042] It should also be appreciated that the present disclosure can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention.
[0043] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0044] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements a, b, and c, and a second embodiment comprises elements b and d, then the inventive subject matter is also considered to include other remaining combinations of a, b, c, or d, even if not explicitly disclosed.
[0045] While a particular form of the invention has been illustrated and described, it will be apparent that various modifications can be made without departing from the spirit and scope of the invention.
[0046] The present invention relates to a mosquito repellent composition. Specifically, the present invention relates to a synergistic herbal mosquito repellent composition comprising a microencapsulated aqueous dispersion of a combination of natural or herbal active ingredients. The composition can be applied on textiles, paper and non-woven surfaces for prolonged mosquito repellent property.
[0047] In some embodiments, the present invention offers a unique and high performing composition of natural active ingredients and its application on textile, paper and non-woven as targeted substrates through the technology of microencapsulation to impart mosquito repellent property properties. The technology of microencapsulation to prepare the microcapsule dispersion is crucial in preparing the aqueous dispersion of the composition of natural active ingredients for application on textiles, paper and non-woven in aqueous processes.
[0048] In some embodiments, the natural active ingredient based on natural oils are selected and a composition is created for its mosquito repellent property properties which are based on performance through sensory perception to give an indication of its presence on the applied substrate and through functional non-aromatic active ingredients which will be present for a longer time duration.
[0049] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition created for its mosquito repellent properties based on performance through sensory perception.
[0050] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition which will be effective for a longer time duration by exhibiting sustained release profile, triggered by methods including but not limiting to pressure and mechanical friction.
[0051] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition comprising better stability to thermal application conditions, and providing resistance against the environmental conditions like light, ultra-violet radiations, storage temperature conditions, biological factors and the like.
[0052] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition, said composition comprises:
a. a base composition comprising
i. a single or a combination of natural active ingredients showing mosquito repellence activity, and.
b. encapsulant,
c. emulsifier,
d. dispersant,
e. foam suppressant, and
f. optionally, one or more additives.
[0053] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises distilled or pharmaceutical grade water to make the composition 100%.
[0054] In an embodiment of the present invention, the additives are selected from but not limited to polyethylene glycol, EMA, diethylene glycol, and urea.
[0055] The natural active ingredient can be a single or combination of natural active ingredients which has the ability to repel mosquitoes with its aromatic and non-aromatic functional nature and including but not limiting to aromatic and non-aromatic functional natural active ingredients which includes but does not limit to citronella oil, lemon eucalyptus oil, eucalyptus globulus oil, cinnamon bark oil, tea tree oil, soybean oil, geraniol oil, neem seed oil, neem seed oil with high azadirachtin content, lemon grass oil and others.
[0056] In an embodiment of the present invention, the natural active ingredient can be an extract from floral, wood, leaf and any other natural source of the respective plants obtained by any process or means including but not limiting to cold pressed, hot pressed and others and which shows performance activity to repel mosquitoes.
[0057] In some embodiments, the microencapsulation technology is used to microencapsulate the composition of natural active ingredient mixed together using an interfacial polymerization technique. The technique is described further in detail along with compositions.
[0058] In some embodiments, the synergistic herbal mosquito repellent composition may further comprise an emulsifier, selected from but not limiting to ethoxylates of castor oil, lauryl alcohol, phenols, fatty alcohols, glycerides and others, copolymers based on but not limiting to propylene maleic anhydride, styrene maleic anhydride, ethylene maleic anhydride, butyl acrylate styrene, homopolymers like polyethylene, polypropylene, polyacrylonitrile, and the like.
[0059] In an embodiment of the present invention, the single or combination of natural active ingredients comprises preferably a concentration of 20 to 99 parts by weight of the base composition, more preferably a concentration of 40 to 98 parts by weight of the base composition.
[0060] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises humectant preferably at a concentration of 1 to 80 parts by weight of the base composition, more preferably at a concentration of 2 to 60 parts by weight of the base composition.
[0061] In an embodiment of the present invention, the base composition comprises the single or combination of natural active ingredients mixed with the humectant.
[0062] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises encapsulant preferably at a concentration of 1 to 20 parts by weight of the base composition, more preferably at a concentration of 2 to 10 parts by weight of the base composition.
[0063] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises emulsifier preferably at a concentration of 0.1 to 10 parts by weight of the base composition and more preferably at a concentration of 0.5 to 5 parts by weight of the base composition.
[0064] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises Polyethylene glycol preferably at a concentration of 0.1 to 0.5% V/V of the whole composition and more preferably at a concentration of 0.2 to 0.35% V/V of the whole composition.
[0065] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises EMA preferably at a concentration of 0.1 to 1% V/V of the whole composition and more preferably at a concentration of 0.7 to 0.8% V/V of the whole composition.
[0066] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises diethylene glycol preferably at a concentration of 0.01 to 0.2% V/V of the whole composition and more preferably at a concentration of 0.1 % V/V of the whole composition.
[0067] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises urea preferably at a concentration of 0.01 to 0.1% V/V of the whole composition and more preferably at a concentration of 0.01 to 0.05% V/V of the whole composition.
[0068] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition may further comprise a natural fragrance in the amount ranging from about 0.01% v/v to about 0.1% v/v of the whole composition.
[0069] In an embodiment of the present invention, the fragrance is Aroma of the essential natural oil selected from lemon, Tea tree, eucalyptus, citronella, lemon grass and a combination thereof.
[0070] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition may further comprise a rheology modifier selected from methyl cellulose (HPMC), CMC, guar gum, xanthan gum, carbopol, gum arabic, cornstarch, gelatin, or the combinations thereof.
[0071] In a preferred embodiment, the present invention provides a synergistic herbal mosquito repellent composition, said composition comprises:
h. a base composition in an amount of 10% to 60% V/V of the whole composition comprising
i. a single or a combination of natural active ingredients having mosquito repellent activity in an amount of 10% to 60% V/V of the base composition, wherein, the natural active ingredients selected from the group consisting of citronella oil, lemon eucalyptus oil, eucalyptus globulus oil, cinnamon bark oil, tea tree oil, soybean oil, geraniol oil, neem seed oil, neem seed oil with high azadirachtin content, and lemon grass oil, and.
i. encapsulant in an amount of 1% to 10% V/V of the whole composition,
j. emulsifier in an amount of 1% to 5% V/V of the whole composition,
k. dispersant in an amount of 0.5% to 5% V/V of the whole composition,
l. foam suppressant in an amount of 0.1% to 1% V/V of the whole composition,
m. optionally, one or more additives, and
n. water to make 100%
[0072] In an embodiment of the present invention, the synergistic herbal mosquito repellent composition comprises distilled or pharmaceutical grade water to make the composition 100%.
[0073] In an embodiment of the present invention, the additives are selected from but not limited to polyethylene glycol, EMA, diethylene glycol, and urea.
[0074] In another embodiment, the present invention provides a method for preparing synergistic herbal mosquito repellent composition disclosed herein, said method comprising the steps of:
e. Mixing natural active ingredients and humectant under a high-speed agitation of minimum 3000 rpm for 10 minutes to obtain a stable composition of microscopic droplets,
f. Microencapsulating the stable composition within capsule wall of the encapsulant to obtain the base composition,
g. Emulsifying by adding an emulsifier to the base composition to obtain aqueous slurry, and
h. Curing the aqueous slurry at 50 to 90 0C to give the strength to the microcapsule walls, followed by cooling to obtain the synergistic herbal mosquito repellent composition
[0075] In an embodiment of the present invention, the surface suitable deposition of the synergistic herbal mosquito repellent composition is textile, woven, or non-woven surface. It should be noted that the suitable surfaces are not limited to the above surfaces, but also can use used in other surfaces including flexible and rigid surfaces known in the art. The synergistic herbal mosquito repellent composition can be applied on said surfaces during processing, finishing, coating, printing, dipping, spraying, and the like.
[0076] In an embodiment of the present invention, the deposition of the herbal mosquito repellent active ingredient from the aqueous media should preferably be 500 mg to 5000 mg per square meter of the surface, more preferably 1120 mg to 4320 mg per square meter of the surface.
[0077] In advantageous embodiments of the present invention, the synergistic herbal mosquito repellent composition has
A. Composition of natural active ingredients: The active ingredient based on natural oils are selected and a composition is created for its mosquito repellent property properties which are based on performance through sensory perception to give an indication of its presence on the applied substrate and through functional non-aromatic active ingredients which will be present for a longer time duration.
B. Better stability to thermal application conditions: The natural active ingredients exhibit stability at relatively lower temperatures compared to the drying temperatures for application on textile, paper, non-woven and related substrates. The composition formed using the microencapsulation technology ensures the heat stability even above 150 0C.
C. Stable aqueous dispersion for application on textiles: The textile substrate is treated with the microcapsule dispersion of the active ingredient.
D. Longevity of the active ingredient during storage: The mosquito repellent natural active ingredient in the microcapsule is protected from the environmental conditions which results in degradation of the active ingredient. The microcapsule being polymeric, completely provides resistance against the environmental conditions like light, ultra-violet radiations, storage temperature conditions, biological factors and others.
E. Enhanced Durability on textiles: Durability is critical when it comes to performance after washing cycles. The composition guarantees higher and better durability.
EXAMPLES
[0078] The present disclosure is further explained in the form of following examples. However, it is to be understood that the foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.
Example 1: Base composition 1
Citronella Oil : 40 gm
Cinnamon Bark Oil : 10 gm
Neem Seed Oil : 40 gm
Tea Tree Oil : 5 gm
Aloe Vera Oil : 5 gm
Process Parameters
Agitator speed: 3000 rpm
Agitation time: 10 min
Example 2: Base composition 2
Citronella Oil : 40 gm
Cinnamon Bark Oil : 40 gm
Neem Seed Oil : 15 gm
Aloe Vera Oil : 5 gm
Process Parameters
Agitator speed: 3000 rpm
Agitation time: 10 min

Example 3: Base composition 3
Citronella Oil : 60 gm
Cinnamon Bark Oil : 10 gm
Neem Seed Oil : 25 gm
Aloe Vera Oil : 5 gm
Process Parameters
Agitator speed: 3000 rpm
Agitation time: 10 min
Observations
All compositions were stable indicating that the composition of oils are compatible and stable microscopic droplets that are formed will be stable during the microencapsulation process to provide good microencapsulation efficiency.
Example 4: Final synergistic herbal mosquito repellent composition 1
Water : 43.53 gm
Composition Example 1 : 50.0 gm
Melamine formaldehyde : 5.29 gm
Polyethylene glycol : 0.33 gm
EMA : 0.72 gm
Diethylene glycol : 0.10 gm
Urea : 0.03 gm
Process Parameters
Agitation speed: 1000 rpm
Agitation time: 4 min
Ageing time: 4 hours
Ageing temperature: 85 deg C
Observations
The microcapsules were formed properly and visible under scanning electron microscope.
The particle size analyzer showed the particle size Dmean = 4.132 µm

Example 5: Final synergistic herbal mosquito repellent composition 2 (urea formaldehyde instead of melamine formaldehyde)
Water : 43.53 gm
Base Composition Example 2 : 50.0 gm
Urea formaldehyde : 4.89 gm
Polyethylene glycol : 0.23 gm
EMA : 0.77 gm
Diethylene glycol : 0.10 gm
Urea : 0.03 gm
Process Parameters
Agitation speed: 1000 rpm
Agitation time: 4 min
Ageing time: 4 hours
Ageing temperature: 85 deg C
Observations
The microcapsules were formed properly and visible under scanning electron microscope (SEM) (FIG. 1A and 1B).
The particle size analyzer showed the particle size Dmean = 8.154 µm
The Final synergistic herbal mosquito repellent composition is then applied on different types of textile substrate, by different methods including but not limiting to finishing, coating, spraying, washing, dip-dry and others and then tested for the mosquito repellent property factor by modified WHO test method.
The application composition of the new composition is based on the deposition of the natural mosquito repellent active ingredient from the aqueous media should preferably be 500 mg to 5000 mg per square meter of the surface, more preferably 1120 mg to 4320 mg per square meter of the surface. Certain examples for the application are mentioned below:
Example 6
New Composition : 40 gm
Water : 1000 ml
Xanthan Gum : 0.2 gm
Process
Substrate: Paper
Method: Knife Coating
Drying time: 3 min
Drying temperature: 130 deg C
Example 7
New Composition : 60 gm
Water : 1000 ml
Binder : 100 gm
Process
Substrate: Textile Woven
Method: Aqueous Finishing
Drying time: 3 min
Drying temperature: 130 deg C
The application substrates were then tested for mosquito repellent property properties by modified WHO/CTD/WHO PES/IC/96.1 test protocol in which 10 Female Anopheles mosquitoes were released per exposure in Excito Repellency chamber made using treated substrate to observe change in behavior in the form of moving away from the treated fabric chamber to untreated one. Data was recorded after 10 min and 30 min and then evaluated.
Test Method: WHO/CTD/WHO PES/IC/96.1
Test process:
10 Female Anopheles mosquitoes were released per exposure in Excito Repellency chamber made using treated substrate to observe change in behaviour in the form of moving away from the treated fabric chamber to untreated one. Data was recorded after 10 min and 30 min and then evaluated.
Application on Textiles:
Fabric A: 25 g/lt. in finishing with drying for 3 min at 1300C Fabric B: 20 g/lt. in finishing with drying for 3 min at 1300C Fabric C: 40 g/lt. in finishing with drying for 3 min at 1300C Fabric D: 60 g/lt. in finishing with drying for 3 min at 1300C
Report Time: 10 min
Table 1: Percentage mosquito repellent property
Sample Identification No of mosquitoes released in chamber No of mosquitoes on treated fabric No of mosquitoes on untreated fabric No of mosquitoes showing mobility % repellency
Fabric A : Final Composition 1 Deposit: 1500 mg per m2 10 2 8 0 80
Fabric B : Final Composition 2 Deposit: 1400 mg per m2 10 2 8 1 70
Fabric C : Final Composition 3 Deposit: 2200 mg per m2 10 1 9 0 90
Fabric D : Final Composition 4 Deposit: 3400 mg per m2 10 0 10 0 100

[0079] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
, Claims:1. A synergistic herbal mosquito repellent composition, said composition comprises:
a. a base composition in an amount of 10% to 60% V/V of the whole composition comprising
i. a single or a combination of natural active ingredients having mosquito repellent activity in an amount of 10% to 60% V/V of the base composition, wherein, the natural active ingredients selected from the group consisting of citronella oil, lemon eucalyptus oil, eucalyptus globulus oil, cinnamon bark oil, tea tree oil, soybean oil, geraniol oil, neem seed oil, neem seed oil with high azadirachtin content, and lemon grass oil, and.
b. encapsulant in an amount of 1% to 10% V/V of the whole composition,
c. emulsifier in an amount of 1% to 5% V/V of the whole composition,
d. dispersant in an amount of 0.5% to 5% V/V of the whole composition,
e. foam suppressant in an amount of 0.1% to 1% V/V of the whole composition,
f. optionally, one or more additives, and
g. water to make 100%
characterized in that:
- the composition is a microencapsulated dispersion of natural active ingredients in aqueous media,
- the microcapsules contained in the microencapsulated dispersion exhibit sustained release profile, triggered by methods including but not limiting to pressure and mechanical friction.
2. The composition as claimed in claim 1, wherein the composition further comprises polyethylene glycol in an amount of 0.1 to 0.5% V/V, EMA in an amount of 0.1 to 1% V/V, diethylene glycol in an amount of 0.01 to 0.2% V/V, and urea in an amount of 0.01 to 0.1% V/V of the whole composition
3. The composition as claimed in claim 1, wherein the emulsifier is selected from the group consisting of castor oil, lauryl alcohol, phenols, fatty alcohols, anhydride copolymers, homopolymers, anhydride copolymers, and maleic anhydride copolymers.
4. The composition as claimed in claim 1, wherein the encapsulant is a pre-polymer selected from the group consisting of urea formaldehyde, melamine formaldehyde, polyurethane, and polyamines.
5. The composition as claimed in claim 1, wherein is based on the deposition of the natural mosquito repellent active ingredient from the aqueous media should be 500 mg to 5000 mg per square meter of a surface.
6. The composition as claimed in claim 1, wherein is based on the deposition of the natural mosquito repellent active ingredient from the aqueous media should be 1120 mg to 4320 mg per square meter of a surface.
7. The composition as claimed in claim 5 or 6, wherein the surface is textile, woven, or non-woven surface.
8. The composition as claimed in claim 1, wherein the composition is applied on the textiles during processing, finishing, coating, printing, or spraying.
9. The composition as claimed in claim 1, wherein the microcapsules has a mean particle size between 2 to 20 µm.
10. A method for preparing synergistic herbal mosquito repellent composition as claimed in claim 1, said method comprising the steps of:
a. Mixing natural active ingredients under a high-speed agitation of minimum 3000 rpm for 10 minutes to obtain a stable composition of microscopic droplets,
b. Microencapsulating the stable composition within capsule wall of the encapsulant to obtain the base composition,
c. Emulsifying by adding an emulsifier to the base composition to obtain aqueous slurry, and
d. Curing the aqueous slurry at 50 to 90 0C to give the strength to the microcapsule walls, followed by cooling to obtain the synergistic herbal mosquito repellent composition as claimed in claim 1.