Field of invention
The present invention relates to a kit for germinating seed(s) and viability
count. Furthermore, it relates to a method for seed(s) germination test and
viability count by using said kit.
5 Background of invention
In agriculture, seed quality is fundamental aspect and is essential for good
crop yield. Good quality seeds minimize the chance of crop failure thereby
reducing economic losses to farmers. Unlabelled seeds from anonymous
source may result in poor field emergence and low planting density. Low
10 quality seeds need extra care and use of more agro inputs like pesticides
and fertilizers. To avoid the crop failure and farmers/buyers from fraudulent
sales, government agencies regulate the seed market norms. The seed
companies are required to carry out seed testing and quality evaluation. The
objective is to predict possible performance in the field and to know planting
15 value of given seeds. Seed testing is an analysis of physical parameters
and physiological qualities of a seed lot, based on a small representative
sample. “Quality” – i.e. physiological quality is a measure of potential
performance under optimal conditions. For farmers, seed testing:
 guarantees that seeds meet minimum quality standards in terms of
20 physical purity and germination percentage;
 minimizes the risk of crop failure; and
 avoids problems resulting from use of seed contaminated with
noxious weeds, seed infested with disease or insects, and seed
having a low viability level
25 Seed germination test is one of the prime and fundamental tests to evaluate
the seed quality. The germination test measures the emergence and
development of the seedling to a stage at which the appearance of its
essential structures indicates whether it can develop further into a
satisfactory plant under favourable conditions in the field. The test is
30 performed to determine the germination potential of particular seed lot, to
3
distinguish viable and non-viable seeds and also to estimate the field
planting value. Thus, germination test is an important parameter to check
the seed quality and it can be carried out at farm level.
Vegetable seeds usually have short life expectancy of 1-6 years if stored
5 under proper storage conditions also. At farm level, due to poor storage
conditions, seeds deteriorate and lose vigour, viability and germination
ability. Seeds of onion, pepper, parsley, parsnip have very short lifespan
and lose viability. In these type of vegetable crops, testing of seed quality
before plantation is important aspect.
10 Onion is considered as one of the most important commercial vegetable
crop produced on large scale in India. India ranks first in terms of area (1.3
Million Ha) and second in production (20.9 Million Tons) after China with
average productivity of 15.86 t/ha. The productivity of onion in India is very
low compared global onion producers like China (22.08 t/ha), Ireland (30.5
15 t/ha), Spain (52.48 t/ha) and USA (66.82 t/ha). The low onion productivity
could be attributed to poor quality seeds, production technologies and
unseasonal rains. As the area of cultivation for onion is increasing YoY, the
demand for high quality seeds is increasing. Availability of viable and
vigorous seeds at the time of planting is paramount for accomplishing
20 production targets. Onion seeds usually lose viability and vigour faster than
other crops. So, onion seed viability is a matter of great concern and efforts
to maintain germination efficiency of stored seeds have significant economic
implications. It is therefore vital for farmers, traders and seed companies
determine seed viability to avoid losses of any kind before sowing. For onion
25 seeds, standard germination test is widely followed as primary method to
determine seed quality.
At farm level, for crops like soybean, wheat, onion etc., farmers use their
own seeds for sowing. With the available resources, farmers perform
germination test using substrata like jute bags, paper or cotton cloth. Many
30 a times, these substrata contain moulds and microbial contaminants that
4
affect the seed germination. Also, conditions like aeration and moisture are
not maintained properly. Due to lack of resources and poor knowledge,
there is always chance of getting wrong germination results.
Several different kinds of testing are available depending on the type of
5 seed to be tested, the conditions of the test, and the potential uses of the
seed. The common tests are the cold germination test, accelerated aging
test, the tetrazolium test and warm germination test. Each test is designed
to evaluate various qualities of the seed.
Warm Germination Test is most common test because it is required by seed
10 laws to appear on the label. The percentage of germinating seed in a Warm
germination test must be printed on the label of the seed if it is to be sold as
seed. The Warm germination test reflects the field emergence potential of a
seed lot under ideal planting conditions. Usually 400 seed from each seed
lot are placed under moist conditions on blotters, rolled towels, or sand and
15 maintained about 75 to 85 degrees F for about seven days in most of the
cases.
Following are the requirements for above mentioned test:
I. Seed counting device:
These devices are required to aid germination testing by minimizing the time
20 spent on planning the seeds as well as to provide proper spacing of the
seed on germination substrata. Counting boards are suitable for medium
and bold sized seeds, while vacuum counter can be, used for small sized
seeds. In the absence of counting devices, the work may be accomplished
manually.
25 II. Paper substrata
The paper substrata are used in the form of top of paper (TP) or between
paper (BP) tests. In most of the laboratories, paper-toweling method (Roll
towel test) is most commonly used for medium sized and bold seeds. The
paper substrata are not reusable.
5
III. Handling of substrata
The accuracy and reproducibility of the germinator result are very much
dependent on the quality of the substrata (paper and sand) used for
germination testing. The germination substrata must meet the following
5 basic requirements:
a) It should be non-toxic to the germinating seedlings. .
b) It should be free from mould sand other microorganisms. .
c) It should provide adequate, aeration and, moisture to the germinating
seeds.
10 d) It should be easy to handle and use.
e) It should make good contrast for judging the seedling.
f) It should be less expensive.
At present the common method of determining seed germination (defined
as growth of the embryo) consists of planting a predetermined number of
15 seeds in light soil or wet towelling and determining the percent with radicle
emergence after a fixed interval of time. The laboratory germination test is
intended to indicate the field planting value of a seed sample (International
Rules for Seed Testing, 1966). Some samples of seeds, however, show a
marked discrepancy between laboratory germination and field emergence.
20 Several attempts have been made in the past to develop tests (in
greenhouse) that would better indicate the field emergence of seed samples
such as germination tests in unsterilized soil and in compost. However these
tests are difficult to standardize and take several days to complete (S.
Matthews and Hort. Res., 1968, Vol. 8 pp. 89-93).
25 Various methods have been proposed for determining the germinating value
of seeds most of which destroy the test seeds.The methods includes sand
germination bed method, paper method and soil method etc.
In the sand germination bed method, a large number of seeds counted out
by hand are deposited in a corresponding large number of germination
6
holes which have been produced likewise manually in sterile sand, again
manually, in germination trays. Moreover, in the filter paper method, seeds
counted out by hand are placed, again manually, in pockets of filter paper.
In both methods, the germination media are moistened with water by hand.
5 After germination has occurred, it is necessary with both methods for the
seedlings to be extracted by hand from the sand or the filter paper pockets,
laid out in heaps of ten and again counted by hand. With the filter paper
method, the counting out must even be carried out for several days in
succession, it being necessary to remove the large shoots, since otherwise
10 it is not possible to establish to which seeds the individual shoots belong.
It is clear that when carrying out the germination tests by human labour with
respect to the two prior known methods, an enormous expense for labour is
necessary, when it is considered. For example, when using the sand
germination bed method about ninety thousand test trays must each be
15 seeded with a hundred seeds and counted out in a germination test period
lasting about seven months and the average hourly capacity of each worker
being about three test trays. On account of this great expenditure on human
labour and of the very large number of tests which take place during the
comparatively short campaign, the germination tests can only be conducted
20 with the smallest possible number of repetitions. Especially in the months
before sowing, the carrying out of the germination tests at the correct time
is often only possible with great difficulties.
There is another possibility of contamination and error while handling of
seeds by manual worker. For example, the wetting of the individual seed
25 samples carried out manually, represents a considerable source of error,
since the seedlings already are in their reactions very sensitive to small
variations in the supply of water. In addition, it is not possible to avoid
counting errors during the numerous counting operations which are
required.
7
US1330112 discloses a method of determining the germinating value of
seed by subjecting selected seeds to the action of a reagent which causes
a contrasting difference in colour between the fit or sound portions and the
unfit or unsound portions of the seed.
5 US 2921598 discloses an apparatus for determining the germination
capacity of seed utilizing a reaction vessel in which selected grains after
having been split longitudinally through the embryo are exposed to a
reaction with an aqueous solution of a compound which causes a dyeing of
the embryo if capable of germinating.
10 US3530372 discloses a method for automatically non-destructively testing
plant seeds and sorting the seeds on the basis of the content of a specific
substance, such as, e.g., water, oil, etc. The weight of a particular seed and
the amount of the given substance contained in it are determined
automatically, from which the specific content of the substance of the seed
15 is calculated automatically, whereupon suitable seeds are selected using as
the criterion whether the specific content is greater than a pre-described
desired value.
Currently in market, there is no germination kit available for any kind of
seeds. Farmers count particular amount of seeds (multiple of 100s) and
20 keep it into moist paper wrap or cloth for 8-10 days to get percent
germination. For small seeds like onion, carrot, cabbage etc. it is difficult to
count seeds and perform germination test.
Therefore while considering above stated problems there is need to
design/develop a kit or an apparatus that simplify germination test process,
25 help farmers to perform germination test properly and to get accurate
results, also maintain the moisture level around 60-70% and easy to
operate.
DESC:Objectives of the present invention
The main object of the present invention is to provide a kit or an apparatus for seed germination test and viability counting.
Other object of the present invention is to provide a method for seed(s) germination test and viability counting.
Another object of the present invention is to provide a kit or an apparatus that helps the farmers to perform germination test properly and to get accurate results.
Another object of the present invention is to provide a kit or an apparatus, that helps the students to engage and understand the seed germination process, to perform germination test for different seeds and to estimate percent germination.
Another object of the present invention is to provide a kit that maintain the moisture level around 60-70% and easy to operate.
Yet another object of the present invention is to provide a kit and/or method that identify/determine seed(s) quality for suitable planting.
Yet further object of the present invention is to provide a method for processing of seeds germination.
Further object of the present invention is to provide a kit and/or method that provide quality seed which meets established quality standards or labelling specifications.
Another object of the present invention is to provide a kit and/or method for germinating seed(s) that provide accurate and reproducible results regarding the quality status of the seed samples submitted.
Summary of the present invention
Main aspect of the present invention is to provide a kit for seed(s) germination test and viability count, the kit comprises:
a tray (100);
a base plate (101);
one or more circle as cultivation media (102);
one or more dot as seed trapping/ inoculating point(103); and
a base plate cover (104).
One aspect of the invention provides a kit for seed(s) germination test and viability count, the kit comprising:
a tray (100);
a base plate (101);
one or more circles (102);
one or more seed inoculation points (103); and
a base plate cover (104);
wherein the base plate (101) keeps spreading the seeds, wherein base plate (101) is placed in the tray (100);
wherein one or more circle (102) identifies the sprouting, germinating seedling;
wherein one or more seed inoculation point (103) appears in the circle (102) that provides information about number of seeds attached to the base plate (101);
wherein the base plate cover (104) covers the tray (100) closed that keeps it moist up to 20 days with single watering.
Another aspect of the invention provides a method for seed germination test and viability count by using the kit, the method comprising:
putting all the seed(s) over the baseplate (101) of the kit;
tapping the kit slowly to spread seeds on the base plate (101); wherein seeds gets attached to the pre-coated glue at one or more seed inoculation point (103) placed on cultivation media (102) which are placed at equidistance on the kit;
removing unattached seeds from the baseplate (101);
placing the baseplate in a tray(100);
moistening the base plate (101);
covering the tray with base plate cover by transparent sheet to avoid water loses and to maintain moisture for germination;
keeping the kit ideal for 8-10 days to observe germination;
identifying and counting germination of individual seed(s); and
calculating the seed germination;
wherein the said method provides % germination in the range of 79% to 91%, seedling length in the range of 30 mm to 48 mm, vigour index in the range of 2370 to 4368.
Further aspect of the invention is to provide a kit for seed(s) germination test and viability count, the kit comprises:
a tray (100);
a base plate (101);
one or more seed trapping/inoculating point (103); and
a base plate cover (104);
wherein base plate (101) is provided with cultivation medium (102) (comprising hydrogel, nutrients etc.) to maintain moist and growth conditions up to 20 days.
Another aspect of the present invention is to provide a kit for seed(s) germination test and viability count, wherein the kit comprises:
a tray (100);
a base plate (101);
one or more circle (102);
one or more dot (103);
a base plate cover (104);
wherein the base plate(101)is kept in the tray and it keeps moist up to 20 days with single watering;
wherein one or more circle(102) to identify the sprouting, germinating seedling;
wherein one or more dot(103) is/are placed in the circle and provides the information about number of seeds attached to the the base plate (103).
Another aspect of the present invention is to provide a method for seed germination test and viability count by using the kit, the method comprising:
putting all the seed(s) over the baseplate of the kit;
tapping the kit slowly to spread seeds on the base plate;wherein 50 or in multiple of 50 seeds gets attached to the precoated glue spots placed at equi-distance on the kit;
optionally, carrying out step (b) if glue spots are clearly visible due to seeds are not attached;
removing unattached seeds from the baseplate;
placing the baseplate in a tray.
moistening the base plate;
covering the tray with base plate by transparent sheet to avoid water loses and to maintain moisture for germination;
keeping the kit ideal for 8-10 days to observe germination;
identifying and counting germination of individual seed(s); and
calculating the seed germination.
Another aspect of the present invention is to provide an educational kit for school students/ research institutes for studying and understanding seed germination, viability and seed vigour.

Brief description of drawing
Figure 1 shows a kit for seed(s) germination and viability count
Figure 2 shows the design of cultivation media with inoculation point and multiple circles used to determine the length of growing seedling. Any point on inner circles can be used to measure the length of growing/ grown seedling from the centre or point of seed attachment. Seedling length gives the seed vigour index (SVI) of that particular seed lot.
Figure 3 is representation of individual circle design but not limited in dimension and number of circles within. The dimensions and the no. of circles will vary with seed type. The innermost white circle varies in diameter from 0.1 to 5 mm. It serves as the substratum for attachment of individual or only one seed per circle. Each innermost white circle contains non-toxic glue that attaches seed. Upon following the standard procedure for seed germination test, the attached seed if viable will germinate.
The circles around the innermost white circle are in increasing diameter order with a constant incremental factor. The diameter of circles with increasing order can be 5mm, 10mm, 15mm, 20mm but not limited to specified dimensions. The circle diameters will vary with seed type. The circle with increasing diameter serves as measurement tool to measure the length of germinating seeds. At the end of germination test, with the help of circles, the seedling length can be easily measured. With percent germination and seedling length, seed vigour index can be calculated.
Figure 4 shows the germinating seedlings
Figure 5 showing the germinating seedlings

Detailed description of the present invention
In the specifications, different types of terms are used for understanding purpose. It does not limit the scope of the present invention.
The definitions of the terms are provided below:
Definitions:
The term ‘seed(s)’ used herein refers to seed is the small, hard part of a plant from which a new plant grows. The seed(s) may include but not limited to vegetable seeds, fruits seeds, spices seeds, grains or grains seeds, onion, rice, wheat, maize, sorghum, barley, peanut, soybean, lentil, common pea, common bean, walnut, pecan, and sunflower. The term ‘seed(s)’ can be used as singular or plural form. Terms ‘seed’ or ‘seed(s)’ can be used interchangeably in the specification.
The term ‘Seed testing’ used herein refers to a test that determine the standards of a seed lot viz., physical purity, moisture, germination and ODV and thereby enabling the farming community to get quality seeds.
The term ‘viability counting’ used herein refers to a method used in to determine the seeds that develop into seedlings.
The term ‘material’ used herein refers to a substance that can be used for making of kit or an apparatus. The material may include but not limited to clay, cardboard, hydrogels, polymers (natural/ synthetic) with additives like fertilizers, plant growth promoters, fungicides, pesticides, (organic, natural, synthetic) and/or combinations thereof.
Main embodiment of the invention provides a kit for seed(s) germination test and viability count, the kit comprising:
a tray (100);
a base plate (101);
one or more circles (102);
one or more seed inoculation points (103); and
a base plate cover (104);
wherein the base plate (101) keeps spreading the seeds, wherein base plate (101) is placed in the tray (100);
wherein one or more circle (102) identifies the sprouting, germinating seedling;
wherein one or more seed inoculation point (103) appears in the circle (102) that provides information about number of seeds attached to the base plate (101);
wherein the base plate cover (104) covers the tray (100) closed that keeps it moist up to 20 days with single watering.

One of the embodiments of the present invention provides a kit for seed(s) germination test and viability count, wherein the kit comprises:
a tray (100);
base plate (101);
one or more circle as cultivation media (102);
one or more dot as seed trapping/ inoculation point(103);
a base plate cover (104).
Another embodiment of the present invention provides a kit for germinating seed test and viability count, wherein the kit comprises:
a tray (100);
base plate (101);
one or more circle as cultivation media (102);
one or more dot as seed trapping/ inoculation point(103);
a base plate cover (104).
wherein the base plate (101) keeps moist till 8-20 days with single watering;
wherein one or more circle (102) to identify the sprouting, germinating seedling;
wherein one or more dot (103) is/are placed in the circle and provides the information about number of seeds attached to the seeds attached to the base plate (101).
Further aspect of the invention is to provide a kit for seed(s) germination test and viability count, the kit comprises:
a tray (100);
base plate (101);
one or more circle as cultivation media (102);
one or more dot as seed trapping/ inoculation point(103);
a base plate cover (104).
wherein base plate (101) is provided with cultivation medium (102) (comprising hydrogel, nutrients etc.) to maintain moist and growth conditions up to 20 days.
Another embodiment of the invention provides a method for seed germination test and viability count by using the kit, the method comprising:
putting all the seed(s) over the baseplate (101) of the kit;
tapping the kit slowly to spread seeds on the base plate (101); wherein seeds gets attached to the pre-coated glue at one or more seed inoculation point (103) placed on cultivation media (102) which are placed at equidistance on the kit;
removing unattached seeds from the baseplate (101);
placing the baseplate in a tray(100);
moistening the base plate (101);
covering the tray with base plate cover by transparent sheet to avoid water loses and to maintain moisture for germination;
keeping the kit ideal for 8-10 days to observe germination;
identifying and counting germination of individual seed(s); and
calculating the seed germination;
wherein the said method provides % germination in the range of 79% to 91%, seedling length in the range of 30 mm to 48 mm, vigour index in the range of 2370 to 4368.

Yet another aspect of the present invention is to provide a method for seed germination test and viability count by using the kit, the method comprising:
putting all the seed(s) over the baseplate (101) of the kit;
tapping the kit slowly to spread seeds on the base plate(101);wherein 50 or in multiple of 50 seeds gets attached to the precoated glue spots placed at equi-distance on the kit;
optionally, carrying out step (b) if glue spots are clearly visible due to seeds are not attached;
removing unattached seeds from the baseplate;
placing the baseplate in a tray (100).
moistening the base plate;
covering the tray holding base plate by transparent sheet to avoid water loses and to maintain moisture for germination;
keeping the kit ideal for 8-10 days to observe germination;
identifying and counting germination of individual seed(s); and
calculating the seed germination.
Another embodiment of the present invention provides a method for germination of seeds and viability count by using the kit, wherein germination of seeds can be calculated by using following formula
Seed germination (%)=(No.of seeds germinated )/(No.of seeds on test kit)×100
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein the base plate may be made up of material including but not limited to clay, cardboard, hydrogels, polymers (natural/ synthetic) with additives like fertilizers, plant growth promoters, fungicides, pesticides, (organic, natural, synthetic) and/or combinations thereof.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein the material has high water holding capacity and it helps to keep the baseplate moist till 8 to 20 days with single watering.
Yet another embodiment of the invention provides the kit for seed(s) germination test and viability count, wherein one or more circles (102) provides cultivation media comprising hydrogel, nutrients.
Yet another embodiment of the invention provides the kit for seed(s) germination test and viability count, wherein the cultivation media maintains moist and growth conditions for the seeds.

Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein base plate has background with colour including but not limited to bright colour.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein the colour may including but not limited to yellow, green, white, red, pink, etc.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein the colour may make visible dark round, square, oval, pentagonal design against the bright background.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein the circles may have numbers including but not limited to 50, 100 or multiple of 50. These circles may be dark coloured (black, blue, brown, etc.). The dark colour of circles is specifically chosen to identify the sprouting, germinating seedling.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein one or more dot within circles may be in no. of 50, 100 or multiple of 50 and may have different colour, for example white, wherein the dot contains non-toxic glue to attach seed.
Other embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein, one or more seed inoculation point (103) as are provided with a glue to attach the seeds.
Further embodiment of the present invention provides a kit for seed(s) germination test and viability counting, wherein, the seed are attached to the glue on seed inoculation point (103) by spreading or by placing manually.

Another embodiment of the present invention provides a kit for seed(s) germination test and viability count, wherein the size and dimension of glue may be designed in such a way that only one seed gets attached to the dot.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability count, wherein the dot glue helps to select 50, 100 seeds in one go. It also provides the information about number of seeds attached to the plate.
Another embodiment of the present invention provides a kit for seed(s) germination test and viability count, wherein the baseplate may have cover and it contains transparent air tight cover to avoid moisture loss and simultaneously visualize the germinating seeds.
Another embodiment of the present invention provides a method for seed(s) germination and viability counting using the kit, wherein the method comprises moistening seed(s) from a dry state for a period of time and at a temperature sufficient to cause the seed(s) germination.
Another embodiment of the present invention provides a method for seed testing and viability counting by using the kit, wherein said method provides information regarding planting value of seed lots from time to time and makes possible for all those who produce, sell and use seeds.
In an embodiment, figure 5 is representation of individual circle design but not limited in dimension and number of circles within. The dimensions and the no. of circles will vary with seed type. The innermost white circle varies in diameter from 0.1 to 5 mm. It serves as the substratum for attachment of individual or only one seed per circle. Each innermost white circle contains non-toxic glue that attaches seed. Upon following the standard procedure for seed germination test, the attached seed if viable will germinate.
Further embodiment of the invention provides one or more circle i.e. cultivation media circles wherein the diameter of the circle is 5 mm to 20 mm.
In an embodiment, the circles around the innermost white circle are in increasing diameter order with a constant incremental factor. The diameter of circles with increasing order can be 5mm, 10mm, 15mm, 20mm but not limited to specified dimensions. The circle diameters will vary with seed type. The circle with increasing diameter serves as measurement tool to measure the length of germinating seeds. At the end of germination test, with the help of circles the seedling length can be easily measured. With percent germination and seedling length, seed vigour index can be calculated.
Another embodiment of the present invention provides a kit or an apparatus that can helps students to engage and understand the seed germination process, perform germination test for different seeds and to estimate percent germination.
In another embodiment of the present invention, the kit can be used in multiple sector including such as but not limited to agriculture sector, agroindustry, educational sector etc.
The following examples are for illustrative purpose only and does not limit the scope of the present invention.
Yet another embodiment of the invention provides the kit for seed(s) germination test and viability count, wherein the seeds are germinated in at least 2 days to 20 days.
Yet another embodiment of the invention provides the kit for seed(s) germination test and viability count, wherein the seeds are germinated in at least 2 days.
Further embodiment of the invention provides the kit for seed(s) germination test and viability count, wherein the kit further comprises additional units selected from artificial intelligence, camera. • Kit with AI camera technology will help to accurately count germinated seeds in a fraction of the time, ensuring reliable results with minimal effort.
Other embodiment of the invention provides the method for seed germination test and viability count, wherein the said method is carried out using the kit.
Example:
Examples are for illustration purpose and does not limit the scope of the invention in any manner

Seed Vigour
Seed vigour is an important quality parameter which needs to be assessed to supplement germination and viability tests to gain insight into the performance of a seed lot in the field or in storage. Seed vigour is defined as “the sum total of those properties of the seed which determine the level of activity and performance of the seed or seed lot during germination and seedling emergence" (ISTA, 1995). In any seed lot, loss of seed vigour relates to a reduction in the ability of seeds to carry out the physiological functions that allow them to perform.
There is no universally accepted vigour test for all kinds of seeds. The determination of following vigour tests will be useful in gaining additional information on seed quality.

Speed of germination:
One hundred seeds each in four replications are planted in recommended substratum for germination. The substratum is kept in a germinator maintained at recommended temperature for the crops in reference. Numbers of seedlings emerging daily are counted from day of planting the seeds in the medium till the time germination is complete. Thereafter a germination index (G.I.) is computed by using the following formula:
G.I.=n/d
Where, n = number of seedling emerging on day ‘d’
d = days after planting
The seed lot having greater germination index is considered to be more vigorous.

Seed lot A: 100
Days of counting 1 2 3 4 5 6 7
No. of seedling emergence on particular day 0 0 12 40 30 10 0

Seed lot B: 100
Days of counting 1 2 3 4 5 6 7
No. of seedling emergence on particular day 0 0 3 28 41 6 2

G.I. of seed lot A =0/1+0/2+12/3+40/4+30/5+10/6+0/7

= 21.6

G.I. of seed lot B =0/1+0/2+3/3+28/4+41/5+6/6+2/7

= 17.4
In this example seed lot A has greater G.I (21.6) than seed lot B (17.4), so seed lot A is more vigorous than seed lot B.

Seed vigour index (S.V.I):
This is calculated by determining the germination percentage and seedling length of the same seed lot. Fifty seeds each in four replications are germinated in towel papers as prescribed for the crop species in germination test. While evaluating the number of normal seedlings at the time of final count, the seedling length of 5 randomly selected seedlings are measured. Seed vigour index is calculated by multiplying germination (%) and seedling length (mm).The seed lot showing the higher seed vigour index is considered to be more vigorous.

Example
Seed Lot % germination Seedling length (mm) Vigour Index
A 86 38 3268
B 91 48 4368
C 79 30 2370

In this example seed lot B is the most vigorous and seed lot C the least vigorous as they have the highest and the lowest values of seed vigour index respectively.
Dated this: May 13h, 2024
,CLAIMS:We claim,
1. A kit for seed(s) germination test and viability count, the kit comprising:
i. a tray (100);
ii. a base plate (101);
iii. one or more circles (102);
iv. one or more seed inoculation points (103); and
v. a base plate cover (104);
wherein the base plate (101) keeps spreading the seeds, wherein base plate (101) is placed in the tray (100);
wherein one or more circle (102) identifies the sprouting, germinating seedling;
wherein one or more seed inoculation point (103) appears in the circle (102) that provides information about number of seeds attached to the base plate (101),
wherein the base plate cover (104) covers the tray (100) closed that keeps it moist up to 20 days with single watering
2. The kit for seed(s) germination test and viability count as claimed in claim 1, wherein one or more circles (102) provides cultivation media comprising hydrogel, nutrients.
3. The kit for seed(s) germination test and viability count as claimed in claim 1, wherein the cultivation media maintains moist and growth conditions for the seeds.
4. The kit for seed(s) germination test and viability count as claimed in claim 1, wherein one or more seed inoculation point (103) as are provided with a glue to attach the seeds.
5. The kit for seed(s) germination test and viability count as claimed in claim 1, wherein the seed are attached to the glue on seed inoculation point (103) by spreading or by placing manually
6. The kit for seed(s) germination test and viability count as claimed in claim 1, wherein the seeds are germinated in at least 2 days.
7. The kit for seed(s) germination test and viability count as claimed in claim 1, further comprises additional units selected from artificial intelligence, camera.
8. A method for seed germination test and viability count by using the kit, the method comprising:
a. putting all the seed(s) over the baseplate (101) of the kit;
b. tapping the kit slowly to spread seeds on the base plate (101); wherein seeds gets attached to the pre-coated glue at one or more seed inoculation point (103) placed on cultivation media (102) which are placed at equidistance on the kit;
c. removing unattached seeds from the baseplate (101);
d. placing the baseplate in a tray(100);
e. moistening the base plate (101);
f. covering the tray with base plate cover by transparent sheet to avoid water loses and to maintain moisture for germination;
g. keeping the kit ideal for 8-10 days to observe germination;
h. identifying and counting germination of individual seed(s); and
i. calculating the seed germination;
wherein the said method provides % germination in the range of 79% to 91%, seedling length in the range of 30 mm to 48 mm, vigour index in the range of 2370 to 4368.
9. The method for seed germination test and viability count as claimed in claim 8, wherein the said method is carried out using the kit as claimed in claim 1.

Dated this 13th May 2024