DESC:FIELD OF THE INVENTION
[001] The present invention relates to a synergistic fungicidal composition for controlling fungal disease in plants, more particularly to a synergistic composition comprising at least two active ingredients along with an antioxidant.

BACKGROUND OF THE INVENTION
[002] A variety of dangerous plant diseases are brought on by fungi. They damage plants by killing cells and causing plant stress. Fungi are spread by wind and water splash, and through the movement of contaminated soil, animals, workers, machinery, tools, seedlings, and other plant material. They enter plants through natural openings such as stomata and through wounds caused by pruning, harvesting, hail, insects, other diseases, and mechanical damage. The prevention of plant infections brought on by fungus-based plant pathogens is crucial for maximizing agricultural productivity. Fungal development on plants or on plant components reduces the production of foliage, fruit, or seeds as well as the overall quality of a cultivated crop, hence it is necessary to keep them under control.
[003] A wide range of fungicidal and fungistatic solutions have been developed for both general and specialized uses due to the significant economic loss. Yet, farmers are continuously looking for better fungicides to successfully manage plant fungus. Combinations of fungicides are frequently used to prevent the development of resistance and advance disease control. To increase the scope and effectiveness of disease management, it is preferable to utilize a combination of active compounds that have a therapeutic effect on plant pathogens as well as invasive and preventative controls.
[004] While many products are commercially available for this purpose, many commercially available fungicides lack bio efficacy and cause more environmental stress. Additionally, it is always preferable to be able to effectively protect crops from diseases brought on by plant pathogens while lowering the amount of chemicals released into the environment. Combinations of fungicides can greatly outperform based on the activity of the individual components in terms of disease control.
[005] Therefore, in view of the above shortcomings, there still exists a need for new and effective insecticidal compositions.

SUMMARY OF THE INVENTION
[006] In one aspect, the present invention is directed to a synergistic fungicidal composition comprising a triazole fungicide, a succinate dehydrogenase inhibitor, at least one antioxidant, and agrochemically acceptable excipients.
[007] In an embodiment, the synergistic fungicidal composition comprises 1.0 wt.% to 60 wt.% of the triazole fungicide, 1.0 wt.% to 60 wt.% of the succinate dehydrogenase inhibitor, 0.1 wt.% to 10 wt.% of at least one antioxidant, and agrochemically acceptable excipients. The wt.% is based on the total weight of the composition.
[008] In another aspect, the present invention is directed to a method for preparing the above synergistic fungicidal composition comprising mixing the following: triazole fungicide, succinate dehydrogenase inhibitor, at least one antioxidant, and agrochemically acceptable excipients.
[009] In an embodiment, the method comprises the following sub-steps:
A) mixing the agrochemically acceptable excipients to obtain a homogenous solution,
B) mixing the triazole fungicide, and the succinate dehydrogenase inhibitor, with the homogenous solution to obtain a pre-mix slurry, and
C) wet grinding the pre-mix slurry to obtain a particle size distribution D50 of less than 5 microns and D90 of less than 10 microns.
[010] In a further aspect, the present invention is directed to a method of controlling and eliminating fungal disease from plants. The method comprises applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attack by fungi, an effective amount of the synergistic fungicidal composition.
[011] In a still further aspect, the present invention is directed to the use of the above synergistic fungicidal composition for controlling fungi.

BRIEF DESCRIPTION OF THE DRAWING
[012] Reference will be made to embodiments of the invention, examples of which may be illustrated in accompanying figures. These figures are intended to be illustrative, not limiting. Although the invention is generally described in context of these embodiments, it should be understood that it is not intended to limit the scope of the invention to these particular embodiments.
Figure 1 is a chart showing leaf greenness (L value) as per international rice research institute (IRRI) leaf colour chart (LCC).

DETAILED DESCRIPTION OF THE INVENTION
[013] Before the compositions and formulations of the present invention are described, it is to be understood that this invention is not limited to particular compositions and formulations described, since such compositions and formulations may, of course, vary. It is also to be understood that the terminology used herein is not intended to be limiting since the scope of the present invention will be limited only by the appended claims.
[014] The terms “comprising”, “comprises” and “comprised of” as used herein are synonymous with “including”, “includes” or “containing”, “contains”, and are inclusive or open-ended and do not exclude additional, non-recited members, elements, or method steps. It will be appreciated that the terms “comprising”, “comprises” and “comprised of” as used herein comprise the terms “consisting of”, “consists” and “consists of”.
[015] Furthermore, the terms “first”, “second”, “third” or “(a)”, “(b)”, “(c)”, “(d)” etc. and the like in the description and in the claims, are used for distinguishing between similar elements and not necessarily for describing a sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances and that the embodiments of the invention described herein. In case the terms “first”, “second”, “third” or “(A)”, “(B)” and “(C)” or “(a)”, “(b)”, “(c)”, “(d)”, “i”, “ii” etc. relate to steps of a method or use or assay there is no time or time interval coherence between the steps, that is, the steps may be carried out simultaneously or there may be time intervals of seconds, minutes, hours, days, weeks, months or even years between such steps unless otherwise indicated in the application as set forth herein above or below.
[016] In the following passages, different aspects of the present invention are defined in more detail. Each aspect so defined may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.
[017] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment but may. Furthermore, the particular features, structures or characteristics may be combined in any suitable manner, as would be apparent to a person skilled in the art from this disclosure, in one or more embodiments. Furthermore, while some embodiments described herein include some, but not other features included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments, as would be understood by those in the art. For example, in the appended claims, any of the claimed embodiments can be used in any combination.
[018] Furthermore, the ranges defined throughout the specification include the end values as well, i.e., a range of 1 to 10 implies that both 1 and 10 are included in the range. For the avoidance of doubt, the applicant shall be entitled to any equivalents according to applicable law.
[019] An aspect of the present invention relates to a synergistic fungicidal composition comprising: a triazole fungicide, a succinate dehydrogenase inhibitor, at least one antioxidant, and agrochemically acceptable excipients.
[020] In an embodiment, the composition includes the aforementioned ingredients in suitable amounts which render the composition effective as a fungicide. For instance, the composition comprises 1.0 wt.% to 60 wt.% of the triazole fungicide; 1.0 wt.% to 60 wt.% of the succinate dehydrogenase inhibitor; and 0.1 wt.% to 10 wt.% of the at least one antioxidant. The agrochemically acceptable excipients are present in amounts to make the total weight of the composition as 100 wt.%. The wt.% of the ingredients in the composition should be considered to be based on the total weight of the composition, unless provided otherwise.
[021] In an embodiment, the triazole fungicide in the composition belongs to the class of demethylation inhibitor (DMI) which inhibits fungal growth through inhibiting the biosynthesis of ergosterol. Suitable Triazole fungicides can be selected from azaconazole, bitertanol, bromuconazole, cyproconazole, difenconazole, diniconazole, epoxiconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, penconazole, propiconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, and prothioconazole.
[022] In an embodiment, the triazole fungicide is selected from cyproconazole, difenconazole, diniconazole, epoxiconazole, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, propiconazole, tebuconazole, triticonazole, and prothioconazol.
[023] In an embodiment, the triazole fungicide is mefentrifluconazole.
[024] Suitable amounts of the triazole fungicide may be added in the composition. In an embodiment, the triazole fungicide is present in an amount ranging between 1.0 wt.% to 60 wt.%, or 1.0 wt.% to 50 wt.%, or 1.0 wt.% to 40 wt.%. In an embodiment, the amount ranges between 5.0 wt.% to 600 wt.%, or 5.0 wt.% to 50 wt.%.
[025] The Succinate dehydrogenase inhibitor (SDHI) fungicides of the present invention belong to a class of broad-spectrum respiration-inhibiting fungicides that have been rapidly adopted by the agricultural industry to manage a range of fungal diseases. Thiazole carboxamides are a group of active compounds within the SDHI family of fungicides. These molecules specifically induce cell death by inhibiting the respiration of targeted fungi by binding to ubiquitin site of mitochondrial succinate ubiquinone reductase (complex II). They are useful in control of many fungal diseases in a wide range of field crops.
[026] In an embodiment, the succinate dehydrogenase inhibitor is selected from thifluzamide, benzovindiflupyr, bixafen, fluindapyr, fluxapyroxad, furametpyr, inpyrfluxam, isopyrazam, penflufen, penthiopyrad, and sedaxane.
[027] In another embodiment, the succinate dehydrogenase inhibitor is thifluzamide.
[028] Suitable amounts of the succinate dehydrogenase inhibitor may be added in the composition. In an embodiment, the succinate dehydrogenase inhibitor is present in an amount ranging between 1.0 wt.% to 60 wt.%, or 1.0 wt.% to 50 wt.%, or 1.0 wt.% to 40 wt.%. In an embodiment, the amount ranges between 5.0 wt.% to 60 wt.%, or 10.0 wt.% to 50 wt.%.
[029] In another embodiment, the at least one antioxidant is selected from sodium sulfite, ascorbic acid, sodium disulfide, carotenoids, vitamins, and elements or compounds of copper, selenium, sulphur, manganese, and zinc. Herein, carotenoids include Beta-Carotene, Lutein, and Lycopene. Further, the compounds of Copper, Selenium, Sulfur, Manganese, and Zinc can be selected from copper sulfate, copper oxychloride, sodium selenite, sodium selenate, sulfur dioxide, manganese sulfate, manganese chloride, zinc oxide, and zinc sulfate. Furthermore, Copper, Selenium, Sulfur, Manganese, and Zinc can also be used in their elemental form as suitable antioxidant in the composition.
[030] Suitable amounts of the antioxidant may be added in the composition. For instance, the at least one antioxidant is present in an amount ranging between 0.1 wt.% to 10 wt.%, or 0.1 wt.% to 8.0 wt.%.
[031] In another embodiment, synergistic fungicidal composition comprises: a triazole fungicide, succinate dehydrogenase inhibitor and at least one antioxidant and agrochemically acceptable excipients.
[032] In an embodiment, the synergistic fungicidal composition comprises: 1.0 wt.% to 60 wt.% of the triazole fungicide; 1.0 wt.% to 60% wt.% of the succinate dehydrogenase inhibitor; 0.1 wt.% to 10 wt.% of at least one antioxidant; and the agrochemically acceptable excipients, wherein the wt.% is based on the total weight of the composition.
[033] In another embodiment, the synergistic fungicidal composition comprises: 1.0 wt.% to 60 wt.% of azaconazole, bitertanol, bromuconazole, cyproconazole, difenconazole, diniconazole, epoxiconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, penconazole, propiconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, and prothioconazol; 1.0 wt.% to 60 wt.% of thifluzamide, benzovindiflupyr, bixafen, fluindapyr, fluxapyroxad, furametpyr, inpyrfluxam, isopyrazam, penflufen, penthiopyrad, and sedaxane; 0.1 wt.% to 10 wt.% of at least one antioxidant; and the agrochemically acceptable excipients, wherein the wt.% is based on the total weight of the composition.
[034] In another embodiment, the synergistic fungicidal composition comprises: 1.0 wt.% to 60 wt.% of cyproconazole, difenconazole, diniconazole, epoxiconazole, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, propiconazole, tebuconazole, triticonazole, and prothioconazol; 1.0 wt.% to 60 wt.% of thifluzamide, benzovindiflupyr, bixafen, fluindapyr, fluxapyroxad, furametpyr, inpyrfluxam, isopyrazam, penflufen, penthiopyrad, and sedaxane; 0.1 wt.% to 10 wt.% of at least one antioxidant; and the agrochemically acceptable excipients, wherein the wt.% is based on the total weight of the composition.
[035] In another embodiment, the synergistic fungicidal composition comprises: 1.0 wt.% to 60 wt.% of cyproconazole, difenconazole, diniconazole, epoxiconazole, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, propiconazole, tebuconazole, triticonazole, and prothioconazol; 1.0 wt.% to 60 wt.% of thifluzamide; 0.1 wt.% to 10 wt.% of at least one antioxidant; and the agrochemically acceptable excipients, wherein the wt.% is based on the total weight of the composition.
[036] In another embodiment, the synergistic fungicidal composition comprises: 1.0 wt.% to 60 wt.% of mefentrifluconazole; 1.0 wt.% to 60 wt.% of thifluzamide; 0.1 wt.% to 10 wt.% of at least one antioxidant; and the agrochemically acceptable excipients, wherein the wt.% is based on the total weight of the composition.
[037] In an embodiment, the composition includes more than one agrochemically acceptable excipient. A person skilled in the art is aware of the suitable agrochemically acceptable excipients that may be included in a typical fungicidal composition. In an embodiment, the agrochemically acceptable excipients are selected from wetting agents, dispersing agents, adjuvants / emulsifiers, polymeric surfactants, antifoam agents, rheology modifiers, antifreeze agents, preservatives, binders/ film forming agents, stabilizing agents, fillers and/or diluents, disintegrating agents, antisettling/ anticaking agents, and combinations thereof. The agrochemically acceptable excipients in a pre-determined ratio aid in improving the physical stability and also prevent the degradation of the composition, thereby leading to long term stability and spectrum activity.
[038] Suitable amounts of the agrochemically acceptable excipients may be added in the composition. In an embodiment, the agrochemically acceptable excipients are added in an amount ranging between 1 wt.% to 80 wt.%. In another embodiment, the amount ranges between 10 wt.% to 80 wt.%, or 20 wt.% to 80 wt.%, or 30 wt.% to 80 wt.%, or 40 wt.% to 80 wt.%, or 50 wt.% to 80 wt.%.
[039] In an embodiment, the wetting agents are selected from, EO/PO block copolymer, polyalkylene oxide block copolymer, salts of dioctyl sulfosuccinate, alcohol alkoxylate diester, sodium n-methyl oleoyl taurate, sodium cocoyl sarcosinate, sodium lauryl sulphate, sodium dioctyl sulfosuccinate, alkyl polyglucoside, naphthalenesulfonic acids, and branched, and linear butyl derivatives of sodium salts.
[040] In another embodiment, the dispersing agents are selected from napthalene sulphonate formaldehyde condensate, amine alkyl benzenesulfonate, sodium ligno sulphonate, blend of naphthalene sulphonate sodium salts, blend of lignin sulphonates sodium salts, ethoxylated lignin sulphonates, linear alkyl benzene sulphonate, tristyrylphenol phosphate, tristearylphenol ethoxylates, acrylic copolymer solution, modified styrene acrylic polymer, ammonium distyrylphenyl ether sulphate, and salts of polystyrene sulphonic acids.
[041] In another embodiment, the adjuvants/emulsifiers are selected from polyoxyethylene alkylamine, polyoxyethylene linear alkyl ether, ethoxylated castor oil, epoxidized soyabean oil, polyoxyethyene alkylamine quaternary, polyoxyethyene tallow ethylmoniumethosulfate, glycerol monostearate, glycerol monooleate, ethoxylated monostearate / distearate / tri stearate ethoxylated monooleate / trioleate, polyoxyethylene branched tridecyl phosphate, polyoxyethylene branched tridecyl phosphate neutralised, polyoxyethylene alkyl ether phosphate, ethoxylated propoxylated sorbitan mono esters, and tallow amine ethoxylate.
[042] In yet another embodiment, the polymeric surfactants are selected from alkoxylated ethylene diamine, butyl block copolymer, alkoxylated polyol ester, butyl polyalkylene oxide block copolymers, polyacrylate copolymer, modified styrene acrylic polymer, and polycarboxylates.
[043] In another embodiment, the antifoam agents are selected from silicon emulsions, and poly dimethyl siloxanes.
[044] In another embodiment, the rheology modifiers are selected from polyester block co-polymer, polysaccharides, polyamide, clay, and silicates.
[045] In still another embodiment, the antifreeze agents are selected from propylene glycol, 1-methoxyl-2-propanol, and butyl cellosolve.
[046] In another embodiment, the preservatives are selected from 1-2-benziisothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, and 2-methyl-4-isothiazolin-3-one.
[047] In yet another embodiment, the stabilizing agents are selected from phosphoric acid monoesters, phosphoric acid diesters, phosphoric acid mono- and di- ester mixture, sodium phosphates, butylated hydroxytoluene, castor oil ethoxylate, ethoxylated hydrogenated castor oil, vegetable oil and epoxidized soyabean oil, and combinations thereof.
[048] In another embodiment, the binders / film-forming agents are selected from the group consisting of hydrophilic polymers, hydrobhobic polymers, poly(vinylpyrrolidone), vinypyrrolidone-vinylacetate, lactose, glucose, fructose, mannose, mannitol, and sucrose.
[049] In another embodiment, the disintegrating agents are selected from sodium chloride, sodium sulphate, ammonium sulphate, sodium carbonate, sodium bicarbonate, and sodium tripolyphosphate (STPP).
[050] In an embodiment, the antisettling/ anticaking agent is amorphous silicon dioxide.
[051] In yet another embodiment, the fillers and/or diluents are selected from starches and their derivatives, sugars and sugar alcohols, silicates, calcium phosphates, calcium sulfate, dextrates, kaolin clay, bentonite clay, attapulgite, diatomaceous earth, magnesium carbonate, polymethacrylates, talc, and salts thereof.
[052] In the present context, water is used as a diluent to dilute the active ingredient to a desired concentration. Water used is purified water and includes deionized water and/or distilled water.
[053] In an embodiment, the composition is formulated in a dosage form selected from the group consisting of suspension concentrate (SC) formulation, Wettable Powder (WP), Water Dispersible Granules (WG), Capsule Suspension (CS), Suspoemulsion (SE), Oil Dispersion (OD), Flowable suspension (FS), water dispersible powder for slurry treatment (WDP), powder for dry seed treatment (DS), Flowable for seed treatment (FS), Emulsiable Concentrate (EC), Emulsion in water (EW), Dual emulsion, Capsule suspension with suspension concentrate (ZC), Capsule suspension with emulsion (ZE), and Capsule suspension with emulsion in water (ZW).
[054] The person skilled in the art is well aware of suitable techniques for making these dosage forms.
[055] Another aspect of the present invention relates to a method for preparing the synergistic fungicidal composition, as above. Accordingly, the embodiments pertaining to the synergistic fungicidal composition are applicable here as well.
[056] In an embodiment, the method comprises at least the step of mixing the following ingredients: triazole fungicide, succinate dehydrogenase inhibitor, at least one antioxidant, and agrochemically acceptable excipients.
[057] In another embodiment, the aforementioned ingredients may be used in suitable and/or predetermined amounts which is capable of imparting enhanced fungicidal and synergistic effect to the overall composition.
[058] In another embodiment, the method comprises the following sub-steps:
A) mixing the agrochemically acceptable excipients to obtain a homogenous solution,
B) mixing the triazole fungicide, and the succinate dehydrogenase inhibitor, with the homogenous solution to obtain a pre-mix slurry, and
C) wet grinding the pre-mix slurry to obtain a particle size distribution D50 of less than 5 microns and D90 of less than 10 microns.
[059] Herein, "grinding" refers to a mechanical process involving the reduction of particle size of the ingredients of the composition. This reduction in particle size 10 may enhance the dispersibility, solubility, or efficacy of the fungicidal agents within the composition. Grinding in this context can encompass various techniques such as milling, pulverizing, or comminution, typically performed using equipment such as mills, crushers, or grinders. The person skilled in the art is well aware of suitable grinding techniques that may be employed for this purpose.
[060] In a further embodiment, the method comprises the following sub-steps:
A) mixing the wetting agents, antifreezing agents, antifoams, preservatives, dispersing agents, antisettling agents, triazole fungicide, and succinate dehydrogenase inhibitor in deionized water to obtain a homogenous solution,
B) wet grinding the homogenous solution till the desired particle size is obtained,
C) transferring the ground slurry in a post-blending tank followed by mixing the antifoams, antioxidants, and rheology modifiers for a duration ranging between 1 h to 4 h.
[061] The aforementioned method steps result in suspension concentrate (SC) dosage form of the composition. Herein, the particle sizes D50 and D90 are determined in accordance with CIPAC MT 187.
[062] The formulations can be of any type known in the sector that is suitable for application onto all types of cultures or crops. These formulations, which can be prepared in any manner known by the skilled person, also form part of the invention.
[063] In one embodiment, for optimal results, preventive applications may also be carried out. Having curative activity, the composition can also be applied after the infection, but the treatments will be carried out in the initial or first stages of development of the fungus. The interval between treatments is adjusted according to the pressure of infection, the development of culture and climatic conditions. The fungi which are combated with the composition of the invention include Sheath blight, Grain discoloration, False smut, Downy mildew, Anthracnose, Rust, Scab, premature leaf fall, Cercospora leaf spot, Leaf spot , Grey Mildew, Anthracnose, Boll rot, Late blight, Early blight, Soft rots, Purple blotch, Stem rot, Alternaria Blight, Powdery mildew, Blister blight, Powdery mildew, Die-back, Anthracnose, Red Rust, Grey blight, Purple blotch, Stemphylium blight, Thielaviopsis rot, Tikka Leaf spot, Yellow stripe rust, Leaf and Fruit rot, Banded sheath blight and Charcoal rot.
[064] Under specific conditions, for example according to the nature of the phytopathogenic fungi or insect to control, a lower dose may offer adequate protection. Certain climatic conditions, resistance or other factors like the nature of the phytopathogenic fungi or damaging insect to be eliminated or the degree of infestation, for example, of the plants with these fungi, may require higher doses of combined active ingredients.
[065] In one embodiment, the synergistic composition of the present invention has uses for controlling variety of Fungal action on various crops such as Paddy, Grapes, Apple, Black gram, Banana Ground Nut, Cotton, Citrus, Ginger, Onion, Potato, Tomato, Cumin, Tea, Chilli, Pome, Mango, Pineapple, Wheat and Maize.
[066] Still another aspect of the present invention relates to a method of controlling and eliminating fungal disease from plants.
[067] In an embodiment, the method comprises applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attack by fungi, an effective amount of the synergistic fungicidal composition, as described above. Accordingly, the embodiments pertaining to the composition are applicable here as well.
[068] Yet another aspect of the present invention relates to the use of synergistic fungicidal composition for controlling fungi. Accordingly, the embodiments pertaining to the composition are applicable here as well.
[069] Advantageously, the present invention provides a composition containing a combination of active ingredients which has several benefits, such as but not limited to, effective control of fungal pathogens, broad-spectrum activity against various fungal species, specificity and selectivity towards target fungi while minimizing harm to non-target organisms, residual activity for prolonged protection, compatibility with other agricultural inputs or industrial processes, stability and longevity under diverse environmental conditions, ease of application, and cost-effectiveness by reducing crop losses or increasing yields. The present composition further provides a reliable, safe, and efficient composition for controlling fungal diseases in agricultural settings, contributing to enhanced productivity, sustainability, and profitability.

EXAMPLES
[070] The present invention is more particularly described in the following examples that are intended as illustration only, since numerous modifications and variations within the scope of the present invention will be apparent to those skilled in the art. Unless otherwise noted, all parts, percentages and ratios reported in the following examples are on a weight basis, and all ingredients used in the examples were obtained or are available from the chemical suppliers.
[071] The following examples illustrate the basic methodology and versatility of the present invention.
[072] Example 1: Mefentrifluconazole 12% + Thifluzamide 18% w/v + Antioxidant SC
[073] The composition was obtained by pre-mixing mefentrifluconazole, thifluzamide along with water, DL-propanediol-(1,2), half quantity of polydimethylsiloxane emulsion, 1,2-benzisothiazole-3-one (Ca.20%), methyl methacrylate graft copolymer, EO/PO block copolymer, polyalkoxylated butyl ether, sodium salt of naphthalene sulphonic acid condensate, amorphous silicon dioxide, into a high-speed dispenser tank. This agitation was continued for 1 hour till homogeneous slurry was obtained. The homogeneous slurry was then passed through wet mill till desired particle size was obtained.
[074] This ground slurry was then transferred into the post blending tank followed balance quantity of polydimethylsiloxane emulsion and L-ascorbic acid. Then polysaccharides were added to the post blending tank and agitation of the whole mixture was continued for 2 hours.
[075] Similarly, compositions for examples 2 to 5 were also prepared. Tables 1a and 1b below summarize the composition details and their physical characteristics.

[076] Table 1a: SC formulations and physical characteristics for examples 1 to 3

Chemical Name

Function/Role
Example 1 Example 2 Example 3
Mefentrifluconazole 12% + Thifluzamide 18% w/v SC Mefentrifluconazole 1% + Thifluzamide 45% w/v SC Mefentrifluconazole 30% + Thifluzamide 5% w/v SC
% w/w % w/w % w/w
Mefentrifluconazole Tech @ 98.8 Active 10.57 0.89 26.45
Thifluzamide Tech @ 97 Active 16.20 40.41 4.49
Methyl methacrylate graft copolymer Dispersing agent 3.00 3.00 3.00
EO/PO block copolymer Wetting agent 2.00 2.00 2.00
Polyalkoxylated butyl ether Dispersing agent 0.50 0.50 0.50
sodium salt of naphthalenesulphonic acid condensate Dispersing agent 0.50 0.50 0.50
Amorphous silicon dioxide Anticaking agent 1.00 1.00 1.00
DL-Propanediol-(1,2) Antifreezing agent 7.00 7.00 7.00
Polydimethylsiloxane emulsion (Silicone antifoam) Antifoam 0.75 0.75 0.75
1,2 -benzisothiazole-3-one (Ca.20%) Preservative 0.10 0.10 0.10
L-Ascorbic acid Antioxidant 0.10 0.10 0.50
Polysaccharides Rheology Modifier 0.24 0.24 0.24
DM water Diluent 58.04 43.51 53.87
Total
100 100 100
PHYSICAL CHARACTERISTICS
S. No. RT 14 Days HT RT 14 Days HT RT 14 Days HT
1. Appearance Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid
2. Mefentrifluconazole (% w/v) 12.45 12.43 1.05 1.03 30.12 30.05
3. Thifluzamide (% w/v) 18.59 18.52 45.12 45.08 5.12 5.08
4. pH (1% w/w aqueous solution) 6.95 6.88 7.01 6.92 6.91 6.76
5. Suspensibility (% w/w) 98.32 97.12 99.12 98.11 96.55 96.12
6. Wet sieve test
(Retention on 45µ test sieve % w/w) 0.07 0.08 0.08 0.1 0.07 0.09
7. Persistent foam (after 1 minute) (mL) 8 10 7 12 9 10
8. Spontaneity of Dispersion (% w/w) 98.88 97.22 98.88 97.22 98.88 97.22
9. Pourability test (Rinsed Residue) 0.4 0.6 0.3 0.5 0.5 0.6
10. Sp Gr 1.158 1.152 1.15 1.151 1.153 1.15
11. Viscosity 547 508 550 522 490 477

[077] Table 1b: SC formulations and physical characteristics for examples 4 to 5

Chemical Name

Function/Role
Example 4 Example 5
Prothioconazole 35% + Thifluzamide 10% w/w SC Difenoconazole 5% + Thifluzamide 30% w/w SC
% w/w % w/w
Thifluzamide Tech @ 97% Active 10.6 31.44
Prothioconazole @ 97% Active 36.60 --
Difenoconazole @ 97% Active -- 5.46
EO/PO block copolymer Wetting agent 1.50 1.50
sodium salt of naphthalenesulphonic acid condensate Dispersing agent 0.50 0.50
tristyrylphenol phosphate Dispersing agent 3.0 3.0
DL-Propanediol-(1,2) Antifreezing agent 6.00 6.00
Polydimethylsiloxane emulsion (Silicone antifoam) Antifoam 0.70 0.70
1,2 -benzisothiazole-3-one(Ca.20%) Preservative 0.10 0.10
L-Ascorbic acid Antioxidant 0.50 0.50
Polysaccharides Rheology Modifier 0.11 0.11
DM water Diluent 40.39 50.69
Total
100 100
PHYSICAL CHARACTERISTICS
S. No. RT 14 Days HT RT 14 Days HT
1. Appearance Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid Off-white to cream colour liquid
2. Prothioconazole (% w/w) 35.4 35.2 NA NA
3. Thifluzamide (% w/w) 10.2 10.1 30.4 30.3
4. Difenoconazole (% w/w) NA NA 5.32 5.28
5. pH (1% w/w aqueous solution) 6.8 6.76 7.2 7
6. Suspensibility (% w/w) 96.4 95.8 98.6 98.4
7. Wet sieve test
(Retention on 45µ test sieve % w/w) 0.08 0.07 0.06 0.07
8. Persistent foam (after 1 minute) (mL) 16 18 12 10
9. Spontaneity of Dispersion (% w/w) 98.2 98.15 97.88 97.23
10. Pourability test (Rinsed Residue) 0.6 0.68 0.56 0.5
11. Sp Gr 1.148 1.143 1.156 1.152
12. Viscosity 448 1726 460 425

[078] From Table 1a and 1b it may be observed that Examples 1-5 are stable and there is no appreciable drop in suspensibility parameter even after accelerated storage study. That there was no significant decrease in the amount of the actives after the storage period, thus indicating that the pesticidal composition prepared of the present disclosure were stable.
[079] Bio-efficacy and phytotoxicity data
[080] The present invention composition was evaluated for bio efficacy and phytotoxicity for Sheath blight, brown spot, false smut, and grain discoloration in Rabi. The findings of the evaluation are summarized below.
[081] Table 2: Bio-efficacy Treatment Details
Sr. No. Product Dose/ha
a.i. g Formulation (g)
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC (Example 1) 60+ 90 500
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 75+90 187.5 + 375
T3 Mefentrifluconazole 40% SC 75 187.5
T4 Thifluzamide 24% SC 90 375
T5 Untreated Check -

[082] Thifluzamide 24% SC combinations: Mefentrifluconazole 40% SC, Thifluzamide 24% SC, were tested at specified concentrations, along with one possible tank mix combinations: Mefentrifluconazole 40% SC + Thifluzamide 24% SC. Additionally, solo individual fungicides, namely Mefentrifluconazole 40% SC, Thifluzamide 24% SC, and an untreated control, were tested against disease complex in Paddy. The fungicides were applied as foliar spray using a Knapsack Sprayer fitted with a hollow cone nozzle. The observations on Percent Disease Index (PDI) of sheath blight and brown spot were calculated. Similarly, the Percent Disease Incidence (PDI) of false smut and Grain discolouration were calculated. The yield was calculated after harvesting. Further, observations on phytotoxicity viz., epinasty, hyponasty, yellowing, necrosis, and leaf tip burning were recorded at 1, 3, 5, 7, 10 and 15 days after fungicide application.
[083] Table 3: Disease Ratings: Paddy Sheath Blight
Scale (Grade) % Infection
0 No infection observed
1 Lesions limited to lower 20% of plant height
3 20-30%
5 31-45%
7 46-65%
9 More than 65%

[084] Table 4: Disease Ratings: Paddy Brown spot
Scale (Grade) % Infection
1 No incidence
2 Less than 1%
3 1-3%
4 4-5%
5 11-15%
6 16-25%
7 26-50%
8 51-75%
9 76-100%

[085] Percent disease index (PDI) was calculated using the following formula:
Sum of all numerical ratings
PDI = --------------------------------------------------------------------- X 100
Total plants observed X Maximum rating scale

[086] Percent disease incidence (PDI) was calculated using the following formula:
No. of infected grains
PDI = --------------------------------------------------------------------- X 100
Total no. of grains
[087] The PDI values were transformed by angular transformation and analyzed statistically. The disease control DC (%) was calculated by the following formula:
Disease % in control - Disease % in treatment
DC (%) = ------------------------------------------------------------- X 100
Disease % in control

[088] Table 5: Bio-efficacy of different fungicide treatments against sheath blight of paddy.
S.NO Treatment Formulation (g/ml)/ha PDI (Percent disease index): Paddy Sheath Blight Disease Reduction Over control
Before spray 15 days after 1st spray 15 days after 2nd spray 15 days after 3rd spray
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 500 2.6
(8.7) 9.3
(17.7) 10.4
(18.8) 11.1
(19.5) 83.5
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 3.0
(9.4) 14.1
(22.9) 14.8
(22.6) 15.2
(22.9) 77.4
T3 Mefentrifluconazole 40% SC 187.5 2.6
(8.7) 15.9
(22.6) 18.5
(25.5) 20.0
(26.6) 70.3
T4 Thifluzamide 24% SC 375 3.0
(9.3) 13.3
(21.3) 14.4
(22.2) 15.6
(23.2) 76.9
T5 Untreated control - 3.0
(9.4) 25.2
(30.1) 31.9
(34.4) 67.4
(55.2)
CD Value @ 0.05 NA NS 2.2 2.3 1.5

[089] In Table 5, figures in parenthesis are arcsine transformed values, PDI is Percent Disease Index, NA is Not available, and NS is non-significant.
[090] As shown in Table 5, before foliar spray of the fungicides the percent disease index (PDI) of sheath blight disease ranged from 2.6 to 3.0 across all treatments and were statistically non-significant, indicating uniformity of trail plot across all the treatments. After 1st foliar spray, Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded minimum (9.3 PDI) sheath blight, which was superior over remaining treatments. Significantly the highest disease index was observed in untreated check plot (25.2 PDI).
[091] A similar trend was observed after 2nd foliar spray. The treatment Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the lowest PDI of 10.4 and it was superior over remaining treatments. The highest disease index was observed in untreated check plot (31.9 PDI). The 15 days after 3rd spray, the lowest PDI (11.1) was observed in Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition, which was superior over remaining treatments.
[092] The highest disease intensity was observed in untreated check plot (67.4 PDI). Among the treatments, the Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded 83.5% disease reduction over control.
[093] Table 6: Bio-efficacy of different fungicides treatments against brown spot of paddy.
S.NO Treatment Formulation (g/ml)/ha Percent disease index (PDI): Paddy Brown Spot Disease Reduction Over control
Before spray 15 days after 1st spray 15 days after 2nd spray 15 days after 3rd spray
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC (Example 1) 500 3.7
(11.1) 3.7
(11.1) 4.8
(12.7) 5.9
(14.1) 87.7
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 3.7
(11.1) 6.3
(14.5) 7.4
(15.8) 8.5
(17) 82.3
T3 Mefentrifluconazole 40% SC 187.5 3.3
(10.5) 9.3
(17.7) 10.4
(18.8) 13.3
(21.4) 72.3
T4 Thifluzamide 24% SC 375 3.7
(11.1) 11.9
(20.1) 13.0
(21.0) 17.8
(24.9) 63.0
T5 Untreated control - 3.7
(11.1) 20.7
(27.1) 32.6
(34.8) 48.1
(43.9) 0.0
CD Value @ 0.05 NA NS 2.2 2.5 1.7

[094] As shown in Table 6, before foliar spray of the fungicides, PDI of rice brown spot disease was ranged from 3.3 to 3.7 across all treatments and were statistically non-significant. After 1st foliar spray, Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the lowest PDI of 3.7 which was superior to remaining treatments. Significantly highest disease index was observed in untreated check plot (20.7 PDI).
[095] A Similar trend was observed after 2nd foliar spray. The treatment Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the lowest PDI of 4.8. The highest disease index was observed in untreated check plot (32.6 PDI). The 15 days after 3rd spray, the lowest PDI (5.9) was observed in Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition, which was superior over remaining treatments.
[096] The highest disease intensity was observed in untreated check plot (48.1 PDI). Among the treatments, the Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the maximum disease reduction over control (87.7 %).
[097] Table 7: Bio-efficacy of different Fungicides treatments against false smut of paddy.
S.NO Treatment Formulation (g/ml)/ha Percent disease incidence (PDI): False smut Disease Reduction Over control
10 Days after 3rd Application 15 Days Before Harvest
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC (Example 1) 500 3.0
(9.8) 4.1
(11.6) 88.5
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 8.5
(17.0) 9.3
(17.7) 73.9
T3 Mefentrifluconazole 40% SC 187.5 11.9
(20.1) 12.6
(20.8) 64.6
T4 Thifluzamide 24% SC 375 16.3
(23.8) 18.5
(25.5) 48.0
T5 Untreated control - 35.6
(36.6) 35.6
(36.6) 0.0
CD Value @ 0.05 NA 2.4 2.5

[098] As shown in Table 7, false smut disease incidence was recorded at 10 days after 3rd foliar spray and 15 days before harvest. The results showed, the lowest PDI of 3.0 was recorded in Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition which was statistically superior over remaining treatments. Maximum disease incidence was recorded in the control plot (35.6 PDI). Among the treatments, Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the lowest mean PDI of 4.1 and the disease reduction over control was 88.5%. The untreated control plot recorded the maximum mean PDI of 35.6.
[099] Table 8: Bio-efficacy of different fungicides treatments against grain discolouration of paddy.
S.NO Treatment Formulation (g/ml)/ha Percent disease incidence (PDI): Grain Discolouration Disease Reduction Over control
10 DA3A 15 Days Before Harvest
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 500 4.1
(11.0) 7.0
(15.4) 84.5
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 9.6
(17.1) 10.7
(19.1) 76.3
T3 Mefentrifluconazole 40% SC 187.5 10.7
(18.1) 15.6
(23.2) 65.5
T4 Thifluzamide 24% SC 375 13.3
(20.2) 19.3
(26) 57.3
T5 Untreated control - 26.7
(29.3) 45.2
(42.2) 0.0
CD Value @ 0.05 NA 1.9 1.0

[0100] As shown in Table 8, grain discolouration disease incidence was recorded 10 days after 3rd foliar spray and 15 days before harvest. The results showed that the lowest PDI of 4.1 was recorded in Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition and it was superior remaining treatments. Maximum disease incidence was recorded in the untreated control plot (26.7 % PDI). Among the treatments, the Mefentrifluconazole 12% + Thifluzamide 18% SC of 500 ml/ha composition recorded the lowest mean PDI of 7.0 and the disease reduction over control was 84.5%. The untreated control plot recorded the maximum mean PDI of 45.2.
[0101] Phytotoxicity:
[0102] Observations were taken on damage caused to plants, if any, by the application of different treatments taking into the account phytotoxic symptoms viz. leaf injury on tips and leaf surface, wilting, vein clearing, necrosis, epinasty and hyponasty on ten plants per plot. The observations were recorded before spray and 1, 3, 5, 7, 10 and 15th day after applications. For phytotoxicity study on leaf injury on tips and leaf surface the scale (0-10) used is given below.
[0103] Table 9: Phytotoxicity Rating Scale (PRS)
Crop response/ Crop injury Rating
0-00 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

[0104] Statistical Analysis:
[0105] Analysis of variance was calculated by using SPSS Software Program.
[0106] Table 10: Phyto-toxicity effect of different fungicide treatments on paddy.
S.NO Treatment Details
Dose *Phytotoxicity (Based on 0-10 Phytotoxicity Rating Scale)
g a.i./ha ml or g/ha Before Spray Days after application (DAA)
1 3 5 7 10 15
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 60+ 90 500 0 0 0 0 0 0 0
T2 Mefentrifluconazole 12% + Thifluzamide 18% SC 120+ 180 1000 0 0 0 0 0 0 0
T3 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 75+90 187.5 + 375 0 0 0 0 0 0 0
T4 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 150 + 180 375+750 0 0 0 0 0 0 0
T5 Untreated Control - - 0 0 0 0 0 0 0

[0107] For phototoxic symptoms- Leaf injury on tips and Leaf surface, Wilting, Necrosis, Epinasty and Hyponasty.
[0108] As shown in Table 10, two-way ready-mix combination was applied at doses of X (500 ml/ha) and 2X (1000 ml/ha) to assess potential phytotoxic effects like leaf injury on tips/surface, wilting, necrosis, hyponasty, and epinasty on Paddy. Phytotoxicity parameters were evaluated prior to spraying and at 1-, 3-, 5-, 7-, 10-, and 15-days post-application. However, no phytotoxicity was observed on the paddy crop. Moreover, there were no adverse effects noted on the paddy crop in the field treated with the fungicide combinations, even at the highest dose of 1000 ml/ha.
[0109] Efficacy against Grain discolouration:
[0110] Grain discolouration disease incidence was recorded 10 days after 3rd foliar spray and 15 days before harvest.


Tr.No Treatment Formulation (g/ml)/ha Percent disease incidence (PDI): Grain Discolouration Disease Reduction Over control
10 DA3A 15 Days Before Harvest
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 500 4.1
(11.0) 7.0
(15.4) 84.5
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 9.6
(17.1) 10.7
(19.1) 76.3
T3 Mefentrifluconazole 40% SC 187.5 10.7
(18.1) 15.6
(23.2) 65.5
T4 Thifluzamide 24% SC 375 13.3
(20.2) 19.3
(26) 57.3
T5 Untreated control - 26.7
(29.3) 45.2
(42.2) 0.0
CD Value @ 0.05 NA 1.9 1.0
[0111] Table 11: Bio-efficacy of different fungicides treatments against grain discolouration of paddy.
Figures in parenthesis are arcsine transformed values.
[0112] As shown in Table 11, the lowest PDI of 4.1 was recorded in Mefentrifluconazole 12% + Thifluzamide 18% SC @ 500 ml/ha and it was superior over remaining treatments. Maximum disease incidence was recorded in the untreated control plot (26.7 % PDI). Among the treatments, the Mefentrifluconazole 12% + Thifluzamide 18% SC @ 500 ml/ha was recorded the lowest mean PDI of 7.0 and the disease reduction over control was 84.5%. The untreated control plot recorded the maximum mean PDI of 45.2.
[0113] Measurement of Leaf greenness:
[0114] The Leaf greenness (L value) is sectioned into four from light (Rating. 2) to dark (Rating. 5) according to international rice research institute (IRRI) leaf colour chart (LCC), as shown in Figure 1. The leaf color is compared with LCC under the same environmental conditions. Measurement of the leaf color was recorded If the L value of leaves is between No. 3 and No. 4, it is counted as 3.5. Comparison of canopy green with LCC is carried out at the place 3 m away from the canopy, under the shade of body. Direct sunlight affects leaf color readings. The color of a single leaf is measured by placing the leaf 1 cm above the color scale. The reading by the color scale of canopy green is more closely correlated with average chlorophyll content of three top leaves than the reading on a single leaf.
[0115] Table 12: Effect of different Fungicides treatments on Plant vigour of Paddy
Tr.No Treatment Formulation (g/ml)/ha LCC ratings
15 Days after 1st Application 15 Days after 2nd Application 15 Days after 3rd Application
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 500 3.8 4.0 4.2
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 2.8 2.8 3.2
T3 Mefentrifluconazole 40% SC 187.5 2.8 2.8 3.0
T4 Thifluzamide 24% SC 375 2.7 2.8 2.8
T5 Untreated control - 2.7 2.7 2.7
CD Value @ 0.05 NA 0.6 0.5 0.3

[0116] As shown in Table 12, the maximum LCC ratings was recorded in Mefentrifluconazole 12% + Thifluzamide 18% SC @ 500 ml/ha and it was statistically superior over remaining treatments (LCC rating 4.2 @ 15 DA3A) indicating application of Mefentrifluconazole 12% + Thifluzamide 18% SC @ 500 ml/ha showing phytotonic effect on Paddy crop. Minimum LCC ratings was recorded in the untreated control plot (LCC rating 2.7).

[0117] Yield:
[0118] Yield data were recorded on a per-plot basis, and treatment-wise yields were calculated and converted into yield per hectare (q/ha) at harvest. The data were then subjected to statistical analysis.
[0119] Table 13: Effect of different fungicides treatments on yield of paddy.
S.No Treatment Details Dose
(ml or g/ha) Paddy
Yield (t/ha)
T1 Mefentrifluconazole 12% + Thifluzamide 18% SC 500 29.4
T2 Mefentrifluconazole 40% SC + Thifluzamide 24% SC (Tank Mix) 187.5 + 375 28.1
T3 Mefentrifluconazole 40% SC 187.5 27.2
T4 Thifluzamide 24% SC 375 27.5
T5 Untreated control - 26.7
CD (P = 0.05) 0.40

[0120] As shown in Table 13, all the treatments significantly increase the yield than Untreated Control (26.7 q/ha). The highest yield was observed in treatment T1 (29.4 q/ha), which was statistically superior to other treatments. T2 recorded yield of 28.1 q/ha followed by T3 (27.2 q/ha) and T4 (27.5 q/ha). Ready mix fungicide treatment (T1) was significantly superior over tank mix and solo fungicide treatments.
[0121] As reported in Tables 5 to 13, the application of Mefentrifluconazole 12% + Thifluzamide 18% SC @ 500 ml/ha has been found to be optimum as its efficacy against sheath blight, brown spot, False smut, and grain discoloration disease. The efficacy of the test fungicides was significantly superior to market standards, including Mefentrifluconazole 40% SC, Thifluzamide 24% SC, and their two-way possible tank mix combination, Mefentrifluconazole 40% SC + Thifluzamide 24% SC.
[0122] Further, the Paddy crop yields were higher with the application of the tested fungicides (9.1% increase over control) with no phytotoxic effects on the rice crop even at the highest dose of 1000 ml/ha. The combination of the tested fungicides exhibited a synergistic effect in disease control. Moreover, to tackle resistance problems and reduce crop losses, these fungicides can be applied safely and efficiently for disease management, compared to using solo, Mefentrifluconazole 40% SC, Thifluzamide 24% SC, and their two-way tank mix combinations individually.
[0123] The foregoing description of the invention has been set merely to illustrate the invention and is not intended to be limiting. While the present invention has been described with respect to certain embodiments, it will be apparent to those skilled in the art that various changes and modification may be made without departing from the scope of the invention as defined in the following claims.
,CLAIMS:WE CLAIM:
1. A synergistic fungicidal composition comprising:
a) a triazole fungicide;
b) a succinate dehydrogenase inhibitor;
c) at least one antioxidant; and
d) agrochemically acceptable excipients.

2. The fungicidal composition as claimed in claim 1, wherein the triazole fungicide is selected from azaconazole, bitertanol, bromuconazole, cyproconazole, difenconazole, diniconazole, epoxiconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, mefentrifluconazole, metconazole, myclobutanil, penconazole, propiconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, and prothioconazole.

3. The fungicidal composition as claimed in claim 1, wherein the succinate dehydrogenase inhibitor is selected from thifluzamide, benzovindiflupyr, bixafen, fluindapyr, fluxapyroxad, furametpyr, inpyrfluxam, isopyrazam, penflufen, penthiopyrad, and sedaxane.

4. The fungicidal composition as claimed in claim 1, wherein the antioxidant is selected from sodium sulfite, ascorbic acid, sodium disulfide, carotenoids, vitamins, and elements or compounds of copper, selenium, sulphur, manganese, and zinc.

5. The fungicidal composition as claimed in claim 1, wherein the agrochemically acceptable excipients are selected from wetting agents, dispersing agents, adjuvants / emulsifiers, polymeric surfactants, antifoam agents, rheology modifiers, antifreeze agents, preservatives, binders/ film forming agents, stabilizing agents, fillers and/or diluents, disintegrating agents, antisettling/anticaking agents, and combinations thereof.

6. The fungicidal composition as claimed in claim 1, wherein the fungicidal composition is formulated in a dosage form selected from suspension concentrate (SC) formulation, Wettable Powder (WP), Water Dispersible Granules (WG), Capsule Suspension (CS), Suspoemulsion (SE), Oil Dispersion (OD), Flowable suspension (FS), water dispersible powder for slurry treatment (WDP), powder for dry seed treatment (DS), Flowable for seed treatment (FS), Emulsiable Concentrate (EC), Emulsion in water (EW), Dual emulsion, Capsule suspension with suspension concentrate (ZC), Capsule suspension with emulsion (ZE), and Capsule suspension with emulsion in water (ZW).

7. The fungicidal composition as claimed in claim 1, wherein the said composition comprises:
a) 1.0 wt.% to 60 wt.% of the triazole fungicide,
b) 1.0 wt.% to 60 wt.% of the succinate dehydrogenase inhibitor,
c) 0.1 wt.% to 10 wt.% of at least one antioxidant, and
d) agrochemically acceptable excipients,
wherein the wt.% is based on the total weight of the composition.

8. A method for preparing the synergistic fungicidal composition as claimed in claims 1 to 7 comprising mixing the following: triazole fungicide, succinate dehydrogenase inhibitor, at least one antioxidant, and agrochemically acceptable excipients.

9. The method as claimed in claim 8, wherein the method comprises the following sub-steps:
a) mixing the agrochemically acceptable excipients to obtain a homogenous solution,
b) mixing the triazole fungicide, and the succinate dehydrogenase inhibitor, with the homogenous solution to obtain a pre-mix slurry, and
c) wet grinding the pre-mix slurry to obtain a particle size distribution D50 of less than 5 microns and D90 of less than 10 microns.

10. A method of controlling and eliminating fungal disease from plants, said method comprising applying to the soil, a locus of infection, a plant and/or propagation material thereof susceptible to attach by fungi, an effective amount of the synergistic fungicidal composition as claimed in claims 1 to 7.

11. Use of the synergistic fungicidal composition as claimed in claims 1 to 7 for controlling fungi.

Dated this 15th Day of June 2023
Indofil Industries Limited
By their Agent & Attorney

(Adheesh Nargolkar)
of Khaitan & Co
Reg No IN-PA-1086