FORM 2
COMPLETE SPECIFICATION
(Under Section 10 and rule 13)
“DEVICE FOR MESURMENT OF SOIL MICROBIAL HEALTH
PARAMETERS AND BIOFERTILIZER BIOPESTISIDE QUALITY
CONTROL” APPLICANT

NAME NATIONALITY ADDRESS
Biome Technologies Pvt Ltd INDIAN Biome Technologies Pvt Ltd.
JyotiPrabha, Saikrupa Ho. Soc. Kedgaon
Industrial Area, Kedgaon, Ahmednagar,
Maharashtra-414005
INVENTORS

SL.NO. NAME NATIONALITY ADDRESS
1 Dr. Prafull Prabhakar Gadge INDIAN Biome Technologies Pvt Ltd.
JyotiPrabha, Saikrupa Ho. Soc.
Kedgaon Industrial Area,
Kedgaon, Ahmednagar,
Maharashtra-414005
2 Miss. Ashwini Navnath Chaure INDIAN Biome Technologies Pvt Ltd.
JyotiPrabha, Saikrupa Ho. Soc.
Kedgaon Industrial Area,
Kedgaon, Ahmednagar,
Maharashtra-414005

Mr. Shivprasad Sagaji Kalamkar INDIAN Biome Technologies Pvt Ltd.
JyotiPrabha, Saikrupa Ho. Soc.
Kedgaon Industrial Area,
Kedgaon, Ahmednagar,
Maharashtra-414005
The following specification particularly describes the invention and the manner, by which is to be performed,
FIELD OF INVENTION
[0001] The present invention disclosure generally relates to the field of soil
assessment, monitoring and more specifically, measurement of soil microbial health
parameters and biofertilizer bio-pesticide quality control.
TECHNICAL FIELD
[0002] The present disclosure of the invention relates to the field of soil assessment
monitoring to soil Microbial count testing. More particularly, the present disclosure
relates to device and method for measurement of soil microbial health parameters
and recommending biofertilizer nutrient additions to the soil.
BACKGROUND OF INVENTION
[0003] Due to continue increase in global human population, urbanization,
industrialization have reduce the urban agricultural land. This changes have been
severely affected by the demand of food and supply ratio whereas continues

increasing word population need a substantial increase in agriculture produce to fulfill the present demand [Tilman et al.2011]. In addition loss of agriculture productivity due to natural and anthropogenic activity leads that land degradation and reduce crop yield. Shift various frequently due to modernization and urbanization hence reduce urban land. farmer are also living this practices because of low cost benefits [A. Kumar et al.2020] intensive use of chemicals fertilizers and pesticides for higher crop production is also detrimental for soil [Kang et al. 2016] and also detrimental effect on soil fertility [Singh 2015] The major challenge I agriculture science is to develop a technique which increase crop yield sustainability. [0004] In ecologically meaningful engineering project are develop in harmony with existing ecosystem for overall environmental benefits [Achaal et al. 2016]. Similarly microbial- best fertilizers can be develop for the benefits of soil while mentioning sustainability. Application of beneficial microbes may be potential alternative to harmful chemicals fertilizers and pesticides. Microbes stand an important role in improving crop productivity and soil management.
[0005] Bacteria are some of the smallest and abodondant microbes in the soil.in a single gram of soil their 60000 different bacteria. There are an estimated yet to be even named, and each has its own particular role and capability. Most live in the to 10 cm of soil where organic matter is present. Soil microbes are the most important candidature for enhancing soil fertility and health. Plant associated with soil

microbes play a crucial role in plant growth and development such as nutrient cycling and crop productivity [Yan et al.2015] plant growth promoting microbes mostly used for plant growth promotion throw various mean such as plant growth regulation and nitrogen fixation [Ahmed et al. 2008] bacteria increase soil fertility through nutrient recycling such as carbon, nitrogen, sulphar, and phosphorus.
[0006] Bacteria are decomposing dead organic matter and release simple compound in the soil. Which can take up by plant nutrient which helps to increase yield and soil productivity. Fertile soil teem with microorganism which directly contribute to the biological fertility of that soil [C. Johns 2017]. In 1 gram of fertile soil which contain 100 million to 1 billion bacteria are present if a bacterial count predict soil is a active and fertile [Ingham 2009]. As soil micro-organism decompose organic matter, they release its nitrogen, phosphorous and other mineral nutrient in forms available to crops. In addition soil microbes improve soil health, soil fertility, soil structure.
[0007] The Green revolution helped us to increase the production necessary to feed the population in corers, however it delivered input intensive practices where farmers told to use chemical fertilizer and pesticides. Including antimicrobials like fungicides and bactericides. Those pesticides contaminate environment and penetrate the soil. They disturb soil micro flora and their activities essential for healthy plant growth. The soil micro flora is first line of defense in plant protection.

Which also affect the stored carbon in soil directly responsible for production, yield
and quality in the farm. As low soil carbon content hampers the nutrient uptake and
stunts the plant growth. Overall disturbed soil micro flora is responsible for low
productivity, low quality, increased diseases & increased production cost? Hence
soil micro flora analysis is necessary...
[0008] The main objective of invention is to provide soil microbial health analysis
and provide soil microbial health analysis and biofertilizer quality control.
OBJECT OF INVENTION
[0009] The main object of the present invention is to provide soil microbial health
analysis and quality control analysis of biofertilizers and microbial pesticides used
in the agriculture field and to develop soil microbial health test data with quantified
viable microbial count by using the device.
SUMMARY OF INVENTION
[0010] The following invention presents a simplified summary of the disclosure in
order to provide a basic understanding to the reader. This summary is not an
overview of the disclosure and it does not identify key/critical elements of the
invention or delineate the scope of the invention. Its sole purpose is to present some
concepts disclosed herein in a simplified form as a prelude to the more detailed
description that is presented late.
[0011] Exemplary embodiments of the present disclosure relate to soil microbial
health monitoring system and methodology deployed to facilitating enhanced soil
assessment.

The present invention disclosure is to provide a method and device that can
understand and estimation of soil microbial health. By using the device is can
determine the color interpretation for quantitative analysis and quickly disseminate
soil test information.
DETAILED DESCRIPTION OF INVENTION
[0012] It is to be understood that the present disclosure is not limited in its
application to the details of construction and the arrangement of components set
forth in the following description or illustrated in the drawings.
The present disclosure is capable of other embodiments and of being practiced or
of being carried out in various ways. Also, it is to be understood that the phraseology
and terminology used herein is for the purpose of description and should not be
regarded as limiting.
[0013] The use of “including”, “comprising” or “having” and variations thereof
herein is meant to encompass the items listed thereafter and equivalents thereof as
well as additional items. The terms “a” and “an” herein do not denote a limitation of
quantity, but rather denote the presence of at least one of the referenced item.
Further, the use of terms “first”, “second”, and “third”, and so forth, herein do not
denote any order, quantity, or importance, but rather are used to distinguish one
element from another.

[0014] The present invention relates to onsite soil microbial health analysis kit -Soilometer. This kit contains of a reagent formulation that changes colour when react with enzymes secreted by bacteria and fungi present in the soil. Explains in FIG. 1 illustrates an exemplary schematic diagram of a device for assessment of soil microbial health and Biofertilizers, Biopestiside in accordance with embodiments of the present disclosure.
[0015] This change in colour is directly proportional to viable bacteria and fungi present in the soil. Similar to pH strip, colour indicators represent soil microbial count and hence the soil microbial health.
[0016] In referring to fig. 2 illustrates an exemplary process flow diagram for method of performing soil microbial health test using the disclosed soil test in accordance with embodiments of the present disclosure for quality control of soil, steps involves Firstly to take 6 ml of solution from bottle 'A' and the entire solution from tube 'B' add in tube 'C'. According to the method of soil test, take one spoon of soil near from the plant or from the field and put in test tube and mix well the mixture then after keep the tube upright in the dark condition for three hours. Finally compare the changed color with the given color chart to understand condition of soil microbial health.

[0017] In reference to FIG.3 illustrates an exemplary process flow diagram for
method of performing Biofertilizers and microbial pesticides quality assessment test
accordance with embodiments of the present disclosure- For quality control of
biofertilizer.
Firstly to take 6 ml of solution from bottle 'A' and the entire solution from tube 'B'
add in tube 'C'. Then add 1ml or 1gm biofertilizer in that tube 'C' and mix it properly
and wait for 3min and Compare the changed color with the given color chart.
[0018] The role of soil microorganisms is important in the agriculture farming, they deliver nutrients to crops, help in uptake of nutrients and protects crops from biotic and abiotic stresses. However excessive chemical fertilizers and pesticides have been reducing count of soil microbes and hence directly affecting productivity and increasing production cost. Thus, even after fertilizer management farmers complain about lower productivity. Therefore, it is necessary to test soil microbial health for above mentioned benefits. The soil microbial health testing in the laboratory is very costly. Expensive for farmers. Here, our Soilometer kit makes it available at with onsite testing. Soilometer android application is in development that will provide advisory for nutrition management to the farmers, based on test results. This application will also provide online training to farmers. This application will be the dedicated platform for the sale of Organic and biological inputs to the farmers.

[0019] Soilometer kit is important to know our soil is alive or not. It delivering us the actual microbial count in soil. Results shows within a 3 hrs. Farmer can easy to handle this technique and all farmer easily affordable these kit because it is economical. Soilometer is On-site testing kit and shows real time results. Its helps to identify soil fertility and health. Which is depends on microbial concentration. Soilometer helps for farmer to decide the weather their current management is robbing future productivity and profits. Currently only lab testing option is available which is costly. So we provide on-site testing kit at affordable price.
[0020] ADVANTAGES OF THE INVENTION
1. The present disclosure provides a device for estimation of available soil microbial population.
2. The present disclosure provides a device for estimation of quality control of biological inputs.
3. This kit will allow farmers to analyze on field real time soil microbial health.
4. Where farmer can take preventive measures such as application of organic & biological fertilizers.
5. This will lead to improved nutrient uptake and plant protection from pests & pathogens.

6. Based on results our online service will guide farmer for suitable applications.
[0021] BRIEF DESCRIPTION OF DRAWINGS.
FIG. 1 illustrates an exemplary schematic diagram of a device for assessment of soil microbial health and Biofertilizers, Biopestiside in accordance with embodiments of the present disclosure.

1 Sr.No 1 Soil microbial health meter 1 Cell count 1
1 Very poor 101
2 Poor 102
3 Average 103
4 Balanced 104 to 105
5 Good 106 to 107
6 Very good 108 to 109

[0022] FIG. 2 illustrates an exemplary process flow diagram for method of performing soil microbial health test using the disclosed soil test in accordance with embodiments of the present disclosure
i) For quality control of soil.


1. Take 6 ml of solution from bottle 'A' and the entire solution from tube 'B' add in tube 'C'.
2. According to the method of soil test, take one spoon of soil near from the plant or from the field and put in test tube.
3. Mix the mixture well.
4. Keep the tube upright in the dark.
5. Wait three hours.
6. Compare the changed color with the given color chart.

[0023] FIG.3 illustrates an exemplary process flow diagram for method of performing Biofertilizer, Biopestiside test accordance with embodiments of the present disclosure ii) For quality control of biofertilizer.


1. Take 6 ml of solution from bottle 'A' and the entire solution from tube 'B' add in tube 'C'.
2. Then add 1ml or 1gm biofertilizer in that tube 'C' and mix it properly.
3. Wait for 3min
4. Compare the changed color with the given color chart.

CLAIMS, We Claim,
1. A soil health assessment system comprising:
a) A primary device comprising of claim 1, wherein the soil sample is mixed in solution and a specific coloring reagent corresponding to the determine soil microbial health and configured to determine quality of biological inputs used in the agriculture such as biofertilizers and microbial pesticides.
2. The soil microbial health assessment system as claimed in claim 2, wherein the primary device further comprises wherein the extracting reagent used for determining quality of biological inputs used in the agriculture such as biofertilizers and microbial pesticides and is estimated calorimetrically.
3. The soil microbial health assessment and monitoring system as claimed in claim 3. further comprising the device wherein the coloring reagent used for determining microbial population is estimated calorimetrically.
4. The soil health assessment and monitoring system as claimed in the device of claim 4, wherein the device gives results within 3 hrs.

5. The soil microbial health assessment and monitoring system as claimed the device of claim 5, wherein the device is on-site testing kit.
6. The soil microbial health assessment and monitoring system as claimed the device of claim 6, wherein the replaced costly and time consuming laboratory test.
7. The soil microbial health assessment and monitoring system as claimed the device of claim 7, wherein the device determine the multiple soil samples or multiple biological agricultural input products at a time.