Description:FIELD OF THE INVENTION
[0001] The present invention relates to a soaping process. Specifically, the present invention relates to a process for the probiotic reactive dyes soaping. Further, the probiotic soaping process is used for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] With an increasing focus on sustainability, the textile industry has witnessed numerous chemical innovations aimed at reducing its environmental impact. Environmentally friendly and biodegradable chemicals have emerged, reducing the ecological footprint of textile production. Moreover, technologies for water and energy-efficient processes, as well as reduced chemical waste, have gained prominence, contributing to a greener textile industry.
[0004] Chemical-based innovations have played a pivotal role in transforming the textile industry, enabling manufacturers to create high-quality, functional, and sustainable textiles. From surface modification and dyeing to flame retardants and antimicrobial properties, these chemical applications have revolutionized fibers and textiles across various sectors. As the industry continues to evolve, research and development in chemical technologies will undoubtedly contribute to more eco-friendly and advanced textile solutions, meeting the demands of consumers and the challenges of a changing world.
[0005] Probiotics have gained significant recognition in recent years for their role in promoting gut health and overall well-being. Probiotics are live microorganisms, mainly bacteria and some yeasts that provide health benefits when consumed in adequate amounts. These beneficial bacteria are known to maintain a balanced gut microbiome, which plays a crucial role in digestion, immune function, and nutrient absorption. They help crowd out harmful bacteria, support the gut lining, and produce beneficial compounds that contribute to overall digestive health. Probiotics have emerged as a valuable component of maintaining a healthy lifestyle, with a positive impact on human health, agriculture, and animal wellbeing.
[0006] Reactive soaping is a crucial post-dyeing process in the textile industry, aimed at improving color fastness and overall fabric performance. After dyeing with reactive dyes, unreacted dye molecules and impurities can remain on the fabric surface, leading to color bleeding and reduced color vibrancy during subsequent washes. Reactive soaping involves the use of specialized soaping agents and washing techniques to remove these residues effectively.
[0007] During the reactive soaping process, the dyed fabric is subjected to multiple washing cycles at controlled temperatures. This step ensures that any unreacted dye molecules are solubilized and removed from the fabric, preventing color bleeding and enhancing color fastness. Additionally, the process imparts a soft and smooth hand feel to the textile, making it comfortable to wear.
[0008] Reactive soaping is vital in achieving textiles with long-lasting and vibrant colors, suitable for various applications like apparel, home textiles, and more. Moreover, proper reactive soaping contributes to sustainable textile production by minimizing dye waste and optimizing water and energy consumption.
[0009] By shortening the reactive soaping process, textile manufacturers can improve production efficiency, reduce environmental impact, and deliver high-quality, colorfast textiles to meet the demands of today's discerning consumers.
[0010] In the existing process chemical is used, needing more wash cycles and ETP load increases. Thus, existing chemical soaping causes a lot of pollution, wastage of water and energy. Effluent load is also increasing. Reactive dyes are commonly used to color textiles and other materials. However, residual unreacted dye after dyeing processes can lead to contamination of wastewater streams. Conventional chemical treatment methods to remove reactive dyes have disadvantages including cost, secondary pollution, and dye bath loss.
[0011] Probiotic microorganisms can provide an alternative, eco-friendly approach to decolorizing residual reactive dyes through metabolism and probiotic transformation. However, current probiotic dye removal processes involve lengthy incubation times.
[0012] Therefore, there is an unmet need in the art to provide an improved probiotic soaping process for efficient, rapid decolorization and removal of residual reactive dyes.

OBJECTIVE OF THE INVENTION
[0013] An objective of the present invention is to provide a process for the probiotic reactive dyes soaping.
[0014] Another objective of the present invention is to provide a probiotic soaping process that is used for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.

SUMMARY OF THE INVENTION
[0015] The present invention relates to a soaping process. Specifically, the present invention relates to a process for the probiotic reactive dyes soaping. Further, the probiotic soaping process is used for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.
[0016] In an aspect, the present invention provides a process for the probiotic reactive dyes soaping comprising the steps of:
a) culturing a probiotic microorganism blend in growth media to produce probiotic microbial biomass and induce enzyme production;
b) introducing the cultured probiotic blend into a dye bath containing residual reactive dye, wherein the residual reactive dye bath is in the soaping stage following dye fixation stage and washing;
c) decolorizing rapidly the residual reactive dye bath by degradation of dye molecules into smaller intermediates by elevated probiotic enzyme activity, wherein complete decolorization occurs at a specified temperature and time period; and
d) adsorbing simultaneously the degraded dye intermediates and remaining intact dye molecules onto the cell surface by the freshly generated probiotic microbial biomass, removing all traces of color and cleaning the dye bath.
[0017] In another aspect of the present invention, the probiotic microorganism blend is selected from bacterial strain, fungal strain or a combination thereof.
[0018] In another aspect of the present invention, the probiotic microorganism blend comprises strains of Bacillus, Lactobacillus, Saccharomyces, Rhizomucor or combinations thereof.
[0019] In another aspect of the present invention, the culturing of probiotic microorganism blend is done at a pH in the range of 5.5 to 7.5.
[0020] In another aspect of the present invention, the culture conditions are optimized to activate probiotic microbial enzyme activity before introduction to the dye bath.
[0021] In another aspect of the present invention, the freshly cultured probiotic blend is stable for six months.
[0022] In another aspect of the present invention, the complete decolorization in step (c) occurs at a temperature in the range of 50 to 70 ?.
[0023] In another aspect of the present invention, the complete decolorization in step (c) occurs within a time period in the range of 20 to 60 minutes.
[0024] In another aspect of the present invention, the probiotic biomass is separated and disposed of safely after decolorizing the soaped dye bath.
[0025] Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments.

DETAILED DESCRIPTION OF THE INVENTION
[0026] The following is a full description of the disclosure's embodiments. The embodiments are described in such a way that the disclosure is clearly communicated. The level of detail provided, on the other hand, is not meant to limit the expected variations of embodiments; rather, it is designed to include all modifications, equivalents, and alternatives that come within the spirit and scope of the current disclosure as defined by the attached claims. Unless the context indicates otherwise, the term "comprise" and variants such as "comprises" and "comprising" throughout the specification are to be read in an open, inclusive meaning, that is, as "including, but not limited to."
[0027] When "one embodiment" or "an embodiment" is used in this specification, it signifies that a particular feature, structure, or characteristic described in conjunction with the embodiment is present in at least one embodiment. As a result, the expressions "in one embodiment" and "in an embodiment" that appear throughout this specification do not necessarily refer to the same embodiment. Furthermore, in one or more embodiments, the specific features, structures, or qualities may be combined in any way that is appropriate.
[0028] Unless the content clearly demands otherwise, the singular terms "a," "an," and "the" include plural referents in this specification and the appended claims. Unless the content explicitly mandates differently, the term "or" is normally used in its broad definition, which includes "and/or."
[0029] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, reaction conditions, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be constructed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.
[0030] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[0031] All processes described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0032] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0033] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[0034] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0035] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified thus fulfilling the written description that follows, and the embodiments described herein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0036] It should also be appreciated that the present invention can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention.
[0037] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0038] In a general embodiment, the present invention relates to a soaping process. Specifically, the present invention relates to a process for the probiotic reactive dyes soaping. Further, the probiotic soaping process is used for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.
[0039] In an embodiment, the present invention provides a probiotic reactive soaping process comprising the steps of:
a) culturing a probiotic microorganism blend;
b) introducing the cultured probiotic blend into a dye bath containing residual reactive dye; and
c) decolorizing rapidly the dye bath by degradation and microbial adsorption.
[0040] In another embodiment, the present invention provides a probiotic soaping process using probiotic microorganisms for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.
[0041] In yet another embodiment, the present invention provides a process for the probiotic reactive dyes soaping comprising the steps of:
a) culturing a probiotic microorganism blend in growth media to produce probiotic microbial biomass and induce enzyme production;
b) introducing the cultured probiotic blend into a dye bath containing residual reactive dye, wherein the residual reactive dye bath is in the soaping stage following dye fixation stage and washing;
c) decolorizing rapidly the residual reactive dye bath by degradation of dye molecules into smaller intermediates by elevated probiotic enzyme activity, wherein complete decolorization occurs at a specified temperature and time period; and
d) adsorbing simultaneously the degraded dye intermediates and remaining intact dye molecules onto the cell surface by the freshly generated probiotic microbial biomass, removing all traces of color and cleaning the dye bath.
[0042] In another embodiment of the present invention, the probiotic microorganism blend is selected from bacterial strain, fungal strain or a combination thereof.
[0043] In another embodiment of the present invention, the probiotic microorganism blend comprises strains of Bacillus, Lactobacillus, Saccharomyces, Rhizomucor or combinations thereof.
[0044] In another embodiment of the present invention, the culturing of probiotic microorganism blend is done at a pH in the range of 5.5 to 7.5.
[0045] In another embodiment of the present invention, the culture conditions are optimized to activate probiotic microbial enzyme activity. The probiotic organisms are activated prior to introduction to the dye bath resulting in rapid decolorization.
[0046] In another embodiment of the present invention, the freshly cultured probiotic blend is stable for six months.
[0047] In another embodiment of the present invention, the complete decolorization in step (c) occurs at a temperature in the range of 50 to 70 ?. Preferably, the complete decolorization occurs at a temperature of 60 ?.
[0048] In another embodiment of the present invention, the complete decolorization in step (c) occurs within a time period in the range of 20 to 60 minutes.
[0049] In another embodiment of the present invention, the probiotic biomass is separated and disposed of safely after decolorizing the soaped dye bath.
[0050] In yet another embodiment, among the numerous probiotic products available for different industries, the present invention creates a new path to probiotics use in the textile industry. This offers a wide range of innovative and effective probiotic solutions.
[0051] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
EXAMPLES
[0052] The present invention is further explained in the form of the following examples. However, it is to be understood that the following examples are merely illustrative and are not to be taken as limitations upon the scope of the invention.
[0053] Procurement of the probiotic microorganisms
Probiotic microorganism samples used in the present invention were received from Greenwave R&D. They were applied successfully to remove hydrolyze dye from the reactive dyed fabric at 60? and took only 20 min.
[0054] Example 1 : Process for the probiotic reactive dyes soaping
A probiotic microorganism blend is selected containing bacterial and/or fungal strains capable of degrading and adsorbing the target reactive dyes. Examples include Bacillus, Lactobacillus, Saccharomyces, and Rhizomucor species. The probiotic blend is cultured in growth media to build up biomass and induce enzyme production. Culture conditions such as temperature, pH, nutrients, and oxygenation are optimized to activate the microbial metabolism. The freshly cultured blend is stable for six months. The activated probiotic blend is introduced to the residual reactive dye bath in the soaping stage following dye fixation and washing. The elevated probiotic activity results in rapid breakdown of dye molecules into smaller intermediates and complete decolorization within 30-60 minutes. Simultaneously, the freshly generated microbial biomass adsorbs degraded dye intermediates and remaining intact dye molecules onto the cell surface. This removes all traces of color and cleans the dye bath. The probiotic biomass can then be separated and disposed of safely. The soaped dye bath is now decolorized and environmentally safe for discharge.
[0055] A skilled artisan will appreciate that the quantity and type of each ingredient can be used in different combinations or singly. All such variations and combinations would be falling within the scope of present disclosure.
[0056] The foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.

ADVANTAGES OF THE PRESENT INVENTION
[0057] The present invention provides a process for the probiotic reactive dyes soaping.
[0058] The present invention provides a probiotic soaping process that is used for efficient removal of residual reactive dyes after a dyeing process by enzymatic degradation and adsorption onto generated microbial biomass.
[0059] The present invention provides an improved probiotic soaping process for efficient, rapid decolorization and removal of residual reactive dyes.
[0060] The present invention provides a short reactive soaping process, thus textile manufacturers can improve production efficiency, reduce environmental impact, and deliver high quality, colorfast textiles to meet the demands of today's discerning consumers.
, Claims:1. A process for the probiotic reactive dyes soaping comprising the steps of:
a) culturing a probiotic microorganism blend in growth media to produce probiotic microbial biomass and induce enzyme production;
b) introducing the cultured probiotic blend into a dye bath containing residual reactive dye, wherein the residual reactive dye bath is in the soaping stage following dye fixation stage and washing;
c) decolorizing rapidly the residual reactive dye bath by degradation of dye molecules into smaller intermediates by elevated probiotic enzyme activity, wherein complete decolorization occurs at a specified temperature and time period; and
d) adsorbing simultaneously the degraded dye intermediates and remaining intact dye molecules onto the cell surface by the freshly generated probiotic microbial biomass, removing all traces of color and cleaning the dye bath.
2. The process as claimed in claim 1, wherein the probiotic microorganism blend is selected from bacterial strain, fungal strain or a combination thereof.
3. The process as claimed in claim 1, wherein the probiotic microorganism blend comprises strains of Bacillus, Lactobacillus, Saccharomyces, Rhizomucor or combinations thereof.
4. The process as claimed in claim 1, wherein the culturing of probiotic microorganism blend is done at a pH in the range of 5.5 to 7.5.
5. The process as claimed in claim 1, wherein the culture conditions are optimized to activate probiotic microbial enzyme activity before introduction to the dye bath in step (b).
6. The process as claimed in claim 1, wherein the freshly cultured probiotic blend is stable for six months.
7. The process as claimed in claim 1, wherein the complete decolorization in step (c) occurs at a temperature in the range of 50 to 70 ?.
8. The process as claimed in claim 1, wherein the complete decolorization in step (c) occurs within a time period in the range of 20 to 60 minutes.
9. The process as claimed in claim 1, wherein the probiotic microbial biomass is separated and disposed of safely after decolorizing the soaped dye bath.