DESC:FIELD OF INVENTION
The present invention relates to hair care compositions. Particularly, the present invention relates to hair care formulations that reduce hair damage and hair loss.

BACKGROUND AND PRIOR ART
Hair care products including shampoos, conditioners and serums aim at reducing hair loss, reducing harmful environmental effects on hair, reducing damage due to uv/visible radiation or oxidative stress and improving hair growth.
Multiple ingredients have been used for this purpose, for example, diaminopyrimidine oxide and caffeine.
Nevertheless, there is an ongoing need for hair care products to reduce hair loss, to reduce hair damage and/or improve hair growth.

OBJECTS OF THE INVENTION
One of the objects of the present invention is to provide a hair care composition that helps in reducing hair loss.
Another object of the present invention is to provide a hair care composition that helps in reducing hair damage.
Yet another object of the present invention is to provide a hair care composition that improves strength of hair.
Yet another object of the present invention is to provide a hair care composition that provides ingredients required for hair growth.
Yet another object of the present invention is to provide a hair care composition that helps in improving hair growth.

Present inventors have found that a mixture of a specific vitamin, certain lipids, and caffeine together with diaminopyrimidine oxide surprisingly helps in providing all ingredients required for hair growth and promotes hair growth.

SUMMARY OF THE INVENTION
According to the present invention, there is provided a hair care composition comprising:
a. 0.0002-2.5% by weight diaminopyrimidine oxide, and;
b. 0.0001-2% by weight sophorolipids, and;
c. 0.0001-1% by weight biotin, and;
d. 0.001-5% by weight caffeine, and;
e. water.

DETAILED DESCRIPTION OF THE INVENTION
According to the present invention, there is provided a hair care composition comprising:
a. 0.0002-5% by weight diaminopyrimidine oxide, and;
b. 0.0001-2% by weight sophorolipids, and;
c. 0.0001-1% by weight biotin, and;
d. 0.001-5% by weight caffeine, and;
e. water.

Diaminopyrimidine oxide
The hair care composition comprises 0.0002 % to 2.5 % by weight diaminopyrimidine oxide.
The hair care formulation comprises diaminopyrimidine oxide at a level preferably greater than 0.001 % by weight, more preferably greater than 0.002 % by weight and further preferably greater than 0.15 % by weight of the composition.
The hair care formulation comprises diaminopyrimidine oxide at a level preferably less than 2.0 % by weight, more preferably less than 1.8 % by weight and further preferably less than 1.5 % by weight of the composition.
Sophorolipids
The hair care composition comprises 0.0001 % to 2 % by weight of sophorolipids.
Sophorolipids are glycolipids having a hydrophobic fatty acid tail and a hydrophilic carbohydrate head sophorose, a glucose-derived di-saccharide with an unusual ß-1,2 bond and can be acetylated on the 6'- and/or 6''- positions. The hydrophobic fatty acid (forming the tail) is C12-C22 fatty acid. The fatty acid may be saturated or unsaturated. One terminal or sub terminal hydroxylated fatty acid is ß-glycosidically linked to the sophorose module. The carboxylic end of this fatty acid is either free (acidic or open form) or internally esterified at the 4'' or in some rare cases at the 6'- or 6''-position (lactonic form).
The hair care formulation comprises sophorolipids at a level preferably greater than 0.0005 % by weight, more preferably greater than 0.001% by weight and further preferably greater than 0.01 % by weight of the composition.
The hair care formulation comprises sophorolipids at a level preferably less than 2 % by weight, more preferably less than 1 % by weight and further preferably less than 0.5 % by weight of the composition.
Biotin
The hair care composition comprises 0.0001 % to 1 % by weight biotin.
Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins, and is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids.
The hair care formulation comprises biotin at a level preferably greater than 0.001 % by weight, more preferably greater than 0.005 % by weight and further preferably greater than 0.01 % by weight of the composition.
The hair care formulation comprises biotin at a level preferably less than 0.5 % by weight, more preferably less than 0.3 % by weight and further preferably less than 0.2 % by weight of the composition.
Biotin is preferably micro-encapsulated, more preferably encapsulated in polysaccharides, preferably starch.
Caffeine
The hair care composition comprises 0.001 % to 5 % by weight of caffeine.
The hair care formulation comprises caffeine at a level preferably greater than 0.002 % by weight, more preferably greater than 0.0025 % by weight and further preferably greater than 0.005 % by weight of the composition.
The hair care formulation comprises caffeine at a level preferably less than 2 % by weight, more preferably less than 1 % by weight and further preferably less than 0.1 % by weight of the composition.
Protein
The term “protein” as used herein means proteins or peptides or amino acids or mixtures thereof. The proteins may be sourced biologically or chemically. Biological sources of proteins include, but are not limited to plants, fungi, bacteria, and animals. Examples of animal proteins or amino acids include, but are not limited to whey protein, egg protein, milk protein, keratins, and collagens, and silk proteins. Proteins or amino acids may be in the form of protein fractions or protein isolates or protein hydrolysates. Hydrolysates may be prepared by acidic/alkaline, chemical, thermal or enzymatic hydrolysis of protein.
The hair care composition comprises 0.001 % to 5 % by weight protein.
The hair care formulation comprises protein at a level preferably greater than 0.005 % by weight, more preferably greater than 0.01 % by weight and further preferably greater than 0.1 % by weight of the composition.
The hair care formulation comprises protein at a level preferably less than 2 % by weight, more preferably less than 1 % by weight and further preferably less than 0.5 % by weight of the composition.
Plant-source protein
According to a particularly preferred aspect of the present invention, protein is a plant-source protein.
The term “plant-source protein” as used herein means proteins or peptides or amino acids or mixtures thereof that are not sourced or derived from animal sources. Preferably the plant-source proteins are from plants, more preferably from grains or legumes. Examples of preferred plant-source amino acids or proteins include corn protein, wheat protein and soya protein.
Plant-source protein are protein isolates, protein hydrolysates, and amino acid fractions obtained from plant sources.
Arginine
The hair care composition comprises 0.0005 % to 5 % by weight arginine. Arginine weight percentage here is in addition to any arginine that may be a part of protein.
The hair care formulation comprises arginine at a level preferably greater than 0.001 % by weight, more preferably greater than 0.005 % by weight and further preferably greater than 0.1 % by weight of the composition.
The hair care formulation comprises arginine at a level preferably less than 2 % by weight, more preferably less than 1.0 % by weight and further preferably less than 0.5 % by weight of the composition.
Serine
The hair care composition comprises 0.0005 % to 5 % by weight serine. Serine weight percentage here is in addition to any arginine that may be a part of protein.
The hair care formulation comprises serine at a level preferably greater than 0.001 % by weight, more preferably greater than 0.005 % by weight and further preferably greater than 0.1 % by weight of the composition.
The hair care formulation comprises serine at a level preferably less than 2 % by weight, more preferably less than 1 % by weight and further preferably less than 0.5% by weight of the composition.
Threonine
The hair care composition comprises 0.0005 % to 5 % by weight threonine. Threonine weight percentage here is in addition to any arginine that may be a part of protein.
The hair care formulation comprises threonine at a level preferably greater than 0.001 % by weight, more preferably greater than 0.005 % by weight and further preferably greater than 0.1 % by weight of the composition.
The hair care formulation comprises threonine at a level preferably less than 2 % by weight, more preferably less than 1 % by weight and further preferably less than 0.5 % by weight of the composition.
Water
The hair care composition comprises water, preferably from 1 % to 95 % by weight of the composition.
The hair care formulation comprises water at a level preferably greater than 1 % by weight, more preferably greater than 5 % by weight and further preferably greater than 10 % by weight of the composition.

The hair care formulation comprises water at a level preferably less than 95 % by weight, more preferably less than 90 % by weight and further preferably less than 85 % by weight of the composition.
Hair care compositions
The hair care compositions according to the present invention include all aqueous hair care compositions including shampoos, conditioners and serums. The different types of hair care compositions are well known in the art, and the hair care compositions of the present invention can be prepared in various formats.
Shampoos
When the hair care composition is a shampoo, water is preferably 40-70% by weight of the composition and more preferably 50-60% by weight of the composition.
Shampoo compositions may preferably comprise surfactants. Surfactants may be anionic, cationic or non-ionic.
Surfactant is preferably from 30% to 40% by weight of the shampoo compositions. Shampoo compositions may preferably comprise various other ingredients well known in the art, such as thickening agents, conditioning agents, humectants, pearlizing agents, herbal extracts, preservatives, chelating agents, fragrance etc.
Thickening agent is preferably 0.5-8% by weight of the shampoo composition. Some non-limiting examples of thickening agents are natural gums such as xanthan gum, hydroxyethyl cellulose, synthetic polymers such as carbomer and superfatting agents such as ethylene glycol monostearate.
Conditioning agent is preferably 2-7% by weight of the shampoo composition. Some non-limiting examples of conditioning agents are quaternium surfactants, quaternium compounds and silicones.
Humectant is preferably 1-10% by weight of the shampoo composition. Some non-limiting examples of humectants are glycerin, sodium PCA and polyols.

Pearlizing agent is preferably 0.5-5% by weight of the shampoo composition. Some non-limiting examples of pearlizing agents are glycol stearates.
Herbal extract is preferably 1-10% by weight of the shampoo composition. Some non-limiting examples of herbal extracts are: Reetha extract, Rosemary extract, Hibiscus extract, Fenugreek extract and Neem extract.
Preservative is preferably 0.5 - 2% by weight of the shampoo composition. Some non-limiting examples of preservatives are phenoxyethanol, imidazolidinyl urea, sodium benzoate and potassium sorbate.
Chelating agent is preferably 0.02 - 1% by weight of the shampoo composition. Some non-limiting examples of chelating agents are: Disodium EDTA and Sodium gluconate.
When hair care composition is a shampoo, it is preferred that the viscosity of the hair care composition is in the range of 1,500 - 3,000 m Pa.s. The viscosity is preferably measured using
a Brookfield RVDV-1 Prime Viscometer using LV torque Spindle no 63 at RPM-30 for 30 sec @25 °C temperature.
Hair Conditioners
When hair care composition is a hair conditioner, water is preferably 60-80 % by weight of the composition and more preferably 65-70 % by weight of the composition.
Hair conditioner compositions may further comprise various other ingredients well known in the art, such as emollients, fatty alcohol/ butters, cationic conditioning agents, humectants, herbal extracts, preservatives, chelating agents, and fragrance.
Emollient is preferably 0.5 - 8% by weight of the conditioner composition. Some non-limiting examples of emollients are plant oils like olive oil, almond oil, synthetic emollients such as Caprylic/capric triglyceride and isopropyl myristate.
Fatty alcohol/butter is preferably 2 - 10 % by weight of the conditioner composition. Some non-limiting examples of fatty alcohols/butters are cetyl alcohol cetostearyl alcohol.
Cationic conditioning agent/emulsifier is preferably 3 - 5 % by weight of the conditioner composition. Some non-limiting examples of cationic conditioning agents are behentrimonium chloride and cetyltrimethylammonium chloride (Quaternary surfactants).
Humectant is preferably 2 - 12 % by weight of the conditioner composition. Some non-limiting examples of humectants are glycerin, polyols and propanediol.
Herbal extract is preferably 1 -10 % by weight of the conditioner composition. Some non-limiting examples of herbal extracts are: Rice extract, Hibiscus extract and fenugreek extract.
Preservative is preferably 0.05 - 2 % by weight of the conditioner composition. Some non-limiting examples of preservatives are: Phenoxyethanol, ethylhexylglycerin, Sodium benzoate and potassium sorbate.
Chelating agent is preferably 0.2-1% by weight of the conditioner composition. Some non-limiting examples of chelating agents are: Disodium EDTA and Sodium gluconate.
When hair care composition is a conditioner, it is preferred that the viscosity of the hair care composition is in the range of 8,000-20,000 m Pa.s. The viscosity is preferably measured using
a Brookfield RVDV-1 Prime Viscometer using Helipath Spindle T-A 91, RPM-3, @25 °C.

Serums
When the hair care composition is a hair scalp serum, water is preferably 70-95 % by weight of the composition and more preferably 80-90 % by weight of the composition. Hair serum compositions may further comprise well known ingredients in the art, such as humectants, thickening agents, preservatives, fragrances.
Humectant is preferably 1-15% by weight of the hair serum composition. Some non-limiting examples of humectants are glycerin, polyols and propanediol.
Thickening agent is preferably 0.1 - 5% by weight of the hair serum composition. Some non-limiting examples of thickening agents are natural gums such as xanthan gum, hydroxyethyl cellulose and synthetic polymers such as carbomer.
Preservative is preferably 0.1 - 2.5% by weight of the hair serum composition. Some non-limiting examples of preservatives are phenoxyethanol, ethylhexylglycerin, sodium benzoate and potassium sorbate.
Soubilizer is preferably 0.5 - 5% by weight of the hair serum composition. Some non-limiting examples of solubilizers are PEG 40 hydrogenated castor oil and polysorbate 20.

Method of preparation of the hair care composition
The hair care compositions according to the present invention can be prepared according to methods well known in the art.
Method of preparation of shampoos
1. Phase A (Water Phase) is prepared by adding ingredients-Chelating agent, humectants, Thickeners/Polymer into a stirred tank and allowing it to hydrate and gel properly. The water phase is heated to 50-80°C.
2. Phase B ingredients- surfactants, pearlizing agents and conditioning agents are slowly added under continuous stirring, until a homogenous mixture is formed.
3. The batch/mixture is allowed to cool down to 20-40°C. Preservatives, fragrance are added then added.
4. At Room temperature, the pH of prepared shampoo is checked and, if required pH is adjusted between 5-6 by adding appropriate pH adjuster.

Method of preparation of conditioners
1. Phase A (Water Phase) is prepared by adding ingredients: Chelating agent, Thickeners/Polymer into a stirred tank and allowing it to hydrate and gel properly. The water phase is heated to 50-80°C.
2. Phase B (Oil Phase) is prepared by adding oils, fatty alcohols, emollients and emulsifiers in a separate vessel and subjected to heating at temperature in the range of 50-80°C.
3. When both the phases attain temperature in the range 50-0°C, the Phase B (Oil phase) is added to Phase A (Water phase) to prepare an o/w emulsion with the help of a homogenizer for 15-45 minutes.
4. The emulsion is allowed to stir and cool, till the temperature reaches 20-40°C.
5. When the temperature reaches 20-40°C, preservatives and fragrance are added and mixed for an additional 5-10 minutes.
6. At Room temperature, pH of the emulsion is checked and adjusted between 4-4.5, by adding appropriate pH adjuster, if required.

Method of preparation of hair serums
1. Phase A (Water Phase) is prepared by adding ingredients-Chelating agent, Thickeners/Polymer into the main vessel and allowing it to hydrate and gel properly.
2. All the other ingredients were added to the main vessel one by one and mixed continuously to form a homogenous mixture.
3. In a separate vessel, the premix of preservative, fragrance and solubilizer is prepared and added to the main vessel.
4. The pH of the serum is checked and adjusted to 5-6, by adding appropriate pH adjuster, if required.

EXAMPLES
The present invention would be demonstrated with the help of examples. The examples are for the purposes of illustration only and do not limit the scope of invention in any way.
Ingredients
Following ingredients were used in the Examples, as tabulated below
Table 1: Ingredients used in the Examples and their origin
Ingredient Supplier and origin
diaminopyrimidine oxide Maruti Futuristic Pharma Pvt Ltd, Karnataka, India
Sophorolipids- BiogodTM (48% sophorolipids, aqueous solution) Godrej Chemicals, Mumbai, India
Encapsulated Biotin (1% biotin encapsulated in maize starch) Sri Krishna Pharmaceutical, Telangana, India
Caffeine Alspure Lifesciences, Haryana, India
Fision KeraVeg 18 (soy protein 5 % - 9.9 %
Wheat protein 5 % - 9.9 %
Arginine 1 % to 4.9 %
Serine 1 % to 4.9 %
Threonine 1% to 4.9 %
Remaining quantity of water Tri-K (Denville, NJ), Galaxy Group, Mumbai, India

Protocol for measurement of proliferation activity
1. Human dermal papilla cells (hDPCs) were plated in a clear bottom, white 96-well plate at a density of 50000 cells/well in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM. After incubation for 24 hours, media was replaced with 50 uL of 1% FBS + DMEM and cells cultured in the serum starved media for another 24 hours. DMEM medium (1% FBS) was used as blank for 24 h.
2. Different combinations of the ingredients (formulation mixes) were prepared at 2X of the final concentration in DMEM + 1% FBS. 50ul of 2X formulations mix (Examples 1-6 and Comparative Examples A-E) each was separately added to the cells and incubated for another 48 hours.
3. After 48 hours, 100 ul of Cell-titer Glo reagent was directly added to the culture medium and luminescence was measured using Biotek multimodal plate reader. Data were analyzed through graphpad prism after normalizing all the test samples with the untreated cells. The results were reported in terms of relative luminescence units (RLU) after normalizing with respect to untreated cells. Values of RLU correspond to proliferation, with higher values of RLU indicating enhanced proliferation with respect to untreated cells whilst lower values of RLU corresponding to relatively low improvement.

Protocol for measurement of intracellular oxidative stress measurement
The cellular oxidative stress level was measured using CellROX green reagent (C10444, ThermoFisher). CellROX Green Reagent is a fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ~ 485/520 nm.
1. Human dermal papilla cells (hDPCs) were plated in a clear bottom, black 96-well plate at a density of 10000 cells/well in 1% FBS in Dulbecco’s modified Eagle’s medium (DMEM).
2. Different combinations of the ingredients (formulation mixes) of the inducing were added at 2X of the final concentration in DMEM + 1% FBS. 50ul of 2X formulations mix (Examples 1-6 and Comparative Examples A-E) was each separately added to the cells, and cells were incubated for another one hour. 1mM H2O2 was used as a positive control for oxidative stress induction.
3. After one hour, 5uM of CellROX dye was directly added to the culture medium and incubated for another 30 minutes. Medium was then removed and washed with PBS three times carefully without disturbing the cells. The fluorescence intensity of the CellROX Green reagent was measured using a multimode microplate reader with excitation and emission wavelengths of 485 and 520 nm, respectively.
4. Data were analyzed through graphpad prism after normalizing all the test samples with the untreated cells. The results were reported as % reduction in ROS (reactive oxygen species) activity. Reduction in ROS activity (%) is indicative of antioxidant properties, with higher % reduction corresponding to better antioxidant properties.

Compositions tested for proliferation activity and oxidative stress
Following compositions were tested according to protocols for measurement of proliferation activity and oxidative stress reduction, as tabulated below. All the studies in the table below were carried out where the composition in the Table were diluted 100 fold (i.e. in the tests, the entire composition was 1% by weight
Table 3: Compositions of Examples 1-4 and Comparative Examples D1-D3, S1-S3, B1-B3, S1-S3 and K1-K3
Ingredient (% by weight)
Example No Diaminopyrimidine oxide Sophorolipid
Encapsulated Biotin Caffeine Fision Keraveg 18
1 0.125 0.001 0.002 0.02 -
2 0.125 0.001 0.002 0.02 2
3 0.125 0.005 0.02 0.2 -
4 0.125 0.005 0.02 0.2 10
D1 0.013 - - - -
D2 0.05 - - - -
D3 0.125 - - - -
S1 - 0.001 - - -
S2 - 0.002 - - -
S3 - 0.005 - - -
B1 - - 0.002 - -
B2 - - 0.006 - -
B3 - - 0.02 - -
C1 - - - 0.02 -
C2 - - - 0.07 -
C3 - - - 0.2 -
K1 - - - - 2
K2 - - - - 5
K3 - - - - 10

Proliferation and reduction in oxidation activity of the compositions tested
The example compositions were tested using proliferation assay and oxidative stress measurement assay according to the protocols given earlier. The results for all the compositions tested are tabulated below.
Table 4: Results of Proliferation (%) and Reduction in ROS activity (%) – carried out with 100X dilution
EXAMPLE No Relative Luminescence Units (RLU) Reduction in ROS (reactive oxygen species) Activity (%)
1 15590.5 60.1
2 21156 63.3
3 19248.5 64.6
4 15354 63.2
D1 4318 14.6
D2 9070 26.9
D3 11152.5 37.5
S1 1367.5 0.9
S2 3932.5 -3.6
S3 4834.5 -8.2
B1 351 -0.6
B2 291 -1.1
B3 453 -1.9
C1 685.5 8.1
C2 2053.5 17.9
C3 3282 31.5
K1 1734.5 -0.5
K2 1159.5 -0.3
K3 502.5 0.6

From the results above, it is clear that the ingredient mixes of Examples 1-4, which are within the scope of the invention significantly improve cell proliferation as compared to that of Comparative Examples D1-D3, S1-S3, B1-B3, C1-C3 and K1-K3 (individual ingredients), which are outside the scope of the present invention. In conclusion, individual ingredients when used alone do not show benefit in proliferation, but when used in combination, provide a surprising and synergistic effect on improving hair dermal papilla cell proliferation, indicative of promoting hair growth.
Furthermore, it is also clear that the ingredient mixes of Examples 1-4, which are within the scope of the invention significantly reduce ROS as compared to that of Comparative Examples D1-D3, S1-S3, B1-B3, C1-C3 and K1-K3 (individual ingredients), which are outside the scope of the present invention. In conclusion, individual ingredients when used alone do not show significant benefit in reducing ROS, but when used in combination, provide a surprising and synergistic effect on reducing ROS, indicating antioxidant benefits.
Examples 5, 6 and 7– Shampoo, conditioner, and hair serum formulations were made as tabulated below
Table 5: Shampoo composition (Example 5)
Ingredient % by weight
Cocoamidopropyl Betaine (CAPB) 18.000
NaLS (SODIUM LAURYL SARCOSINATE) 14.000
DECYL GLUCOSIDE (P-2000) 5.000
Acrypol ET 1 3.700
COCO APPLE AMINO ACID 2.000
GLYCERIN 2.000
KERA VEG 18 .100
Rosemary Extract .010
CAFFIENE .002
Kopexil (Aminexil)
Diaminopyrimidine Oxide .002
BIOGOD .001
BIOTIN .001
Water Make up to 100

Table 6: Conditioner composition (Example 6)
Ingredient % by weight
ISO PROPYL MYRISTATE (IPM) 4.500
CETYL ALCOHOL (Ginol-16) 4.500
CETOSTEARYL ALCOHOL (GINOL 1618 TA) 2.500
OLIVE OIL 2.500
GLYCEROL MONO STEARATE - SE 2.500
GLYCERIN 1.500
Behentrimonium Chloride (KDMP) 1.500
SALCARE SUPER AT1 1.500
MIRASIL EMUL 6960 1.500
MIRASIL EMUL 6788 1.500
KERA VEG 18 .100
Rosemary Extract .010
CAFFIENE .002
Kopexil (Aminexil) .002
BIOGOD .001
BIOTIN .001
Water Make up to 100

Table 7: Hair serum composition (Example 7)
Ingredient % by weight
PROPYLENE GLYCOL (PG) 8.00
ZEMEA (Propanediol) 8.00
BUTYLENE GLYCOL 4.00
SODIUM PCA 2.00
GLYCERIN 1.50
KERA VEG 18 2.00
CAFFIENE .04
Kopexil (Aminexil) .04
BIOGOD .02
BIOTIN .02
Water Make up to 100

Table 8: Results of Reduction in ROS activity (%) for shampoo and conditioner formulations (Example 5 and 6)
Test concentration in the assay 0.001% 0.01% 0.1% 1%
Reduction in ROS Activity (%) Example 5 (Shampoo) 23 25.2 44 35
Example 6 (Conditioner) 24.8 37.1 62.1 56.5

Compositions of Example 5 and 6 (shampoo and conditioner, respectively) showed a dose dependent reduction in H2O2 induced oxidative stress in human dermal papilla cells when tested at test concentrations ranging from 0.001-1%.
Table 9: Results of improvement in hair proliferation:
The proliferation experiments were carried out using composition of Example 7 tested at 0.001%, 0.01%, 0.1% and 1% in the proliferation assay and the results are tabulated below
Test concentration (Composition of Example 7) 0.001% 0.01% 0.1% 1%
% increase in proliferation 32 57 85 73

It can be seen that the composition of Example 7 provides enhancement in the proliferation activity when tested at a range of test concentrations between 0.001-1%
Consumer study using compositions of Example 5, 6 and 7 (shampoo, conditioner, and hair serum, respectively)
Study group:
For hair shampoo (example 5) - 70 panelists
Males (21) & Females (49)
Age :18 to 35 years.

For hair conditioner (Example 6) - 50 panelists
Males (15) & Females (35)
Age :18 to 35 years.

For hair serum (Example 7) - 70 panelists
Males (21) & Females (49)
Age :18 to 35 years.

Methodology:
Shampoo (Example 5), Conditioner (Example 6), and hair serum (Example 7) were given to the panelists with instructions to use apply them twice for evaluation.
The compositions were evaluated for the following attributes:
Texture
Fragrance
Colour
Appearance
Foam Quality
Ease of Application
Cleansing Capability
Ease of Removal
Did not cause Dryness to scalp
Did not cause Irritation to scalp & eyes
Made hair bouncy
Made hair smooth
Made hair shinny
Did not make hair feel sticky
Made hair soft
Made hair moisturized
Post use feel
Based on all the above, the panelists gave a likeability and overall experience score. The results are reported as % of panelists who liked the product and had good overall experience.

Table 10: % of panelists who liked the product and overall experience
Composition Likeability (% of panelists who liked the product) Overall experience (% of panelists who had good overall experience)
Example 5 84 83
Example 6 84 84
Example 7 74 83

,CLAIMS:WE CLAIM:

1. A hair care composition comprising:
a. 0.0002-2.5% by weight diaminopyrimidine oxide, and;
b. 0.0001-2% by weight sophorolipids, and;
c. 0.0001-1% by weight biotin, and;
d. 0.001-5% by weight caffeine, and;
e. water.
2. A hair care composition as claimed in claim 1 comprising 0.001-5% by weight protein.
3. A hair care composition as claimed in any one of the preceding claims wherein said protein is a plant-source protein.
4. A hair care composition as claimed in any one of the preceding claims comprising 0.0005-5% by weight arginine.
5. A hair care composition as claimed in any one of the preceding claims comprising 0.0005-5 % by weight threonine.
6. A hair care composition as claimed in any one of the preceding claims comprising 0.0005-5% by weight serine.