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Method Of Detecing Nucleic Acid Sequences And Point Mutations Using A Nucleic Acid Lateral Flow Assay (Nalfa)
Abstract: ABSTRACT “METHOD OF DETECTING NUCLEIC ACID SEQUENCES AND POINT MUTATIONS USING A NUCLEIC ACID LATERAL FLOW ASSAY (NALFA)” The present disclosure provides a method for detecting nucleic acid using a combination of nucleic acid amplification technique and NALFA. The method comprises amplifying the target nucleic acid with a labelled forward/reverse primer in excess and unlabelled forward/reverse primer in limited amount, subjecting the singly labelled single stranded amplification to hybridisation with a labelled probe to generate a labelled product which is then detected on a NALFA strip. The present disclosure also provides a method for detecting point mutations using the same method as above. However, this method involves use of a molecular beacon for hybridising to the mutated sequence and also requires the NALFA assay to be carried out at elevated temperatures. The methods of the present disclosure may be used for detecting microorganisms, infections and/or genetic disorders. The present disclosure also provides kits for carrying out the methods described herein.
Notices, Deadlines & Correspondence
Patent Information
Applicants
Indian Institute of Science
Inventors
1. Priyanka Agarwal
2. Bhushan Jayant Toley
Specification
DESC:ABSTRACT
“METHOD OF DETECING NUCLEIC ACID SEQUENCES AND POINT MUTATIONS USING A NUCLEIC ACID LATERAL FLOW ASSAY (NALFA)”
The present disclosure provides a method for detecting nucleic acid using a combination of nucleic acid amplification technique and NALFA. The method comprises amplifying the target nucleic acid with a labelled forward/reverse primer in excess and unlabelled forward/reverse primer in limited amount, subjecting the singly labelled single stranded amplicon to hybridization with a labelled probe to generate a bilabeled product which is then detected on a NALFA strip. The present disclosure also provides a method for detecting point mutations using the same method as above. However, this method involves use of a molecular beacon for hybridizing to the mutated sequence and also requires the NALFA assay to be carried out at elevated temperatures. The methods of the present disclosure may be used for detecting microorganisms, infections and/or genetic disorders. The present disclosure also provides kits for carrying out the methods described herein. ,CLAIMS:AS FILED PDF DOCUMENTS
Documents
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 202341038914-STATEMENT OF UNDERTAKING (FORM 3) [06-06-2023(online)].pdf | 2023-06-06 |
| 2 | 202341038914-PROVISIONAL SPECIFICATION [06-06-2023(online)].pdf | 2023-06-06 |
| 3 | 202341038914-POWER OF AUTHORITY [06-06-2023(online)].pdf | 2023-06-06 |
| 4 | 202341038914-FORM FOR SMALL ENTITY(FORM-28) [06-06-2023(online)].pdf | 2023-06-06 |
| 5 | 202341038914-FORM 1 [06-06-2023(online)].pdf | 2023-06-06 |
| 6 | 202341038914-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [06-06-2023(online)].pdf | 2023-06-06 |
| 7 | 202341038914-EDUCATIONAL INSTITUTION(S) [06-06-2023(online)].pdf | 2023-06-06 |
| 8 | 202341038914-DRAWINGS [06-06-2023(online)].pdf | 2023-06-06 |
| 9 | 202341038914-DECLARATION OF INVENTORSHIP (FORM 5) [06-06-2023(online)].pdf | 2023-06-06 |
| 10 | 202341038914-Proof of Right [07-07-2023(online)].pdf | 2023-07-07 |
| 12 | 202341038914-Sequence Listing in PDF [06-06-2024(online)].pdf | 2024-06-06 |
| 13 | 202341038914-FORM-8 [06-06-2024(online)].pdf | 2024-06-06 |
| 14 | 202341038914-FORM 18 [06-06-2024(online)].pdf | 2024-06-06 |
| 15 | 202341038914-DRAWING [06-06-2024(online)].pdf | 2024-06-06 |
| 16 | 202341038914-CORRESPONDENCE-OTHERS [06-06-2024(online)].pdf | 2024-06-06 |
| 17 | 202341038914-COMPLETE SPECIFICATION [06-06-2024(online)].pdf | 2024-06-06 |