DESC:FIELD OF THE INVENTION
The present invention relates to a stable, synergistic insecticidal composition and a process for preparation thereof. Further, the present invention relates to a method for controlling pests in agricultural crops.

BACKGROUND OF THE INVENTION
The protection of crops and their produce from insect pest damage is essential for the enhancement of agriculture produce. Even after the availability of alternative types of pest control, chemical control remains an important tool for the prevention and control of pests in agriculture, especially insect pests. Insecticides of varied types and structural groups have been reported in the literature and a large number are in commercial use. However, judicious use of such chemical pesticides is absolutely needed to protect the environment and natural ecosystems. The insecticide toxin remains active in the plant for many weeks, protecting the crop season-long.

Previously people have tried many alternatives and option to overcome this problem and as a result developed poly mixture of insecticide, use of non-toxic ingredients and developing novel formulations which provides effective amount of the insecticide and at the required part only. However, the use of poly mixture containing large number of insecticides poses a problem in many ways like preparing formulation of multiple insecticides with different chemical properties and behaviour and physical properties. It also creates challenges for the formulator in terms of compatibility and stability of all the insecticides along with the used excipients in the formulation.

Flonicamid is chemically known as N-(cyanomethyl)-4-(trifluoromethyl) pyridine-3- carboxamide, having a chemical structure as below:

Flonicamid is a member of the pyridinecarboxamide class of chemistry. It is a systemic insecticide that controls pests by contact and ingestion. It is used to control aphids,reenhouse whiteflies, tobacco whitefly, tea green leafhopper, brown planthopper, and various thrips.

Diafenthiuron chemically known as 1-tert-butyl-3-[4-phenoxy-2, 6-di (propan-2- yl) phenyl] thiourea, having a chemical structure as below:
Diafenthiuron is a chemical compound that belongs to the thiourea group and is an insecticide and acaricide. It is a broad spectrum, non-systemic insecticide that kills larvae, nymphs, and adults by contact and/or stomach action. It is effective against phytophagous mites and other sucking pests like aphids, whiteflies, spidermites, diamondback moth, thrips, and jassids.

Cyantraniliprole is chemically known as 5-bromo-2-(3-chloropyridin-2-yl)-N-[4-cyano-2- methyl-6-(methylcarbamoyl) phenyl] pyrazole-3-carboxamide, having a chemical structure as below:

Cyantraniliprole is a diamide insecticide that is approved for use in the United States, Canada, China, and India. It is a ryanodine receptor (RyR) modulator which kills insects through unregulated activation of RyR. Insects exposed to Cyantraniliprole become lethargic, paralyzed, and eventually die.

A combination of pesticides may be applied sequentially, as a tank application or as a ready- to-use-composition. A tank mix of pesticides in many cases results in a heterogeneous solution and suffers from several other problems like difficulty in handling, sedimentation etc. hence, and a pre-mix composition is much preferred. However, it is a known fact that not all actives are compatible with each other, and therefore rigorous efforts are required to develop a stable, synergistic, efficacious formulation.

There is therefore a continued need to develop insecticidal compositions that provides good bio efficacy against target pests, provides a composition with high synergistic action, an advantageous spectrum of activity, an increased safety profile, improved physio-chemical properties, a combination where the dosage of the active ingredients is reduced as compared to the individual recommended dosage, to avoid excess loading of the toxicant to the environment and to negligibly impact environmental safety or compositions which display a more rapid onset of action or which have long lasting residual activity or which enable a reduction in the number of applications and/or a reduction in the application rate of the compounds and compositions required for effective control of a phytopathogen, thereby enabling beneficial resistance-management practices.

Surprisingly, the inventors of the present invention have found that the novel and synergistic composition of Flonicamid, Diafenthiuron, Cyantraniliprole and Soy protein, as described herein, provides a solution to the above-mentioned problems.

AU2005319651B2 discloses mixtures of anthranilamide invertebrate pest control agents. It discloses a composition comprising Cyantraniliprole and at least one invertebrate pest control agent. However, it does not disclose an insecticidal composition which provides a broad- spectrum control of insect pests in cotton crop.

The present invention does not fall within the scope of the above-mentioned state of the art as it relates to a unique combination of Flonicamid, Diafenthiuron, Cyantraniliprole and Soy protein. The references mentioned in the prior art do not disclose any three-way mixture nor the synergistic composition according to the present invention, nor the concentration ranges of the components in the mixture.

OBJECT(S) OF THE INVENTION
It is an object of the present invention to provide a novel, synergistic insecticidal composition.

It is another object of the present invention to provide an insecticidal composition comprising Flonicamid, Diafenthiuron, Cyantraniliprole and Soy protein.

It is another object of the present invention to provide a synergistic insecticidal composition for control of pests in various agricultural crops.

It is yet another object of the present invention to provide an insecticidal composition having enhanced efficacies at reduced dosage.

It is another object of the present invention to provide an insecticidal composition for enhancing crop yield without having any phyto toxicity.

It is yet another object of the present invention to provide a process for preparing the synergistic insecticidal composition.

It is yet another object of the present invention to provide a method for controlling pests in various agricultural crops without causing substantial harm to the crops.

SUMMARY OF THE INVENTION
In one aspect, the present invention provides a novel, stable, synergistic, environmentally safe, cost-effective insecticidal composition comprising:
Flonicamid in an amount ranging from 10-24% w/w;
Diafenthiuron in an amount ranging from 10-40%w/w;
Cyantraniliprole in an amount ranging from 2-20% w/w;
Soy protein in an amount ranging from 0.001-2%w/w; and
at least one agriculturally acceptable excipient.

In another aspect, the present invention provides a process for the preparation of said insecticidal composition, comprising:
obtaining a homogeneous mixture of anti-freeze agent, wetting cum dispersing agent in Demineralized Water (D.M water);
adding antifoam, Flonicamid technical, Diafenthiuron technical and Cyantraniliprole technical to the homogenous mixture of step (a) to obtain a homogenous mass;
grinding said homogenous mass in sand mill until mean particle size of less than 5 microns is obtained;
adding Soy protein, biocide and 2% water suspension of a thickener under low stirring and mix well.

In yet another aspect, the present invention provides a method for combating insect pests in crops of useful plants without causing substantial harm to the crops, which comprises treating the useful plants, seeds or cuttings thereof or the locus of the useful plants with an insecticidally active amount of an insecticidal composition.

In yet another aspect, the present invention provides a method for combating insect pests in crops of useful plants without causing substantial harm to the crops, which comprises treating the useful plants, seeds or cuttings thereof or the locus of the useful plants with an insecticidally active amount of an insecticidal composition.

TECHNICAL ADVANCEMENTS OF THE INVENTION
The insecticidal composition as disclosed in the present invention comprises Flonicamid, Diafenthiuron, Cyantraniliprole and Soy protein as described herein above and has several associated technical advantages including but not limited to:
enhanced pest control;
synergistic effect;
plant health benefits; and
reduced environmental impact.

DETAILED DESCRIPTION OF THE INVENTION

Abbreviations
D.M. Demineralized Water
RTU Ready to Use
SC Suspension Concentrates
A.I/a.i Active Ingredients
IC No. of insects in control plots
IT No. of insects in treated plot
DAA Days After Application
QS Quantum satis

Those skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described herein. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and all combinations of any two or more of said steps or features.

Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of the ordinary skills in the art to which the invention belongs. For further guidance, term definitions may be included to better appreciate the teaching of the present invention.

As used herein, the term “plant” refers to any plant or part thereof including serial and subterranean parts of the plant. It is contemplated that the parts of the plant may be for example, flowers, fruits or vegetables, shoots, leaves, needles, stalks, stems, fruiting bodies, seeds also roots and that parts of the plants may or may not be attached to the remainder of the plant.

The term “disease control” as used herein denotes control and prevention of a disease. Controlling effects include all deviation from natural development, for example: killing, retardation, decrease of the pests. The term „plants? refers to all physical parts of a plant, including seeds, seedlings, saplings, roots, tubers, stems, stalks, foliage and fruits.

The term “insects” as used herein, includes all organisms in the class “Insecta.”

The term “Insecticidal” as used herein refers to the ability of a pesticide to increase morality or inhibit the growth rate of insects.

The term “pests” as used herein refers to any living thing—a plant, an animal, or a microorganism that has a negative effect on humans.

It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.

It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components, or groups thereof.

The terms “weight percent”, “wt-%”, “percent by weight”, “%” by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100. It is understood that, as used here, “percent “, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc.

As used herein, the term “agriculturally acceptable excipient” refers to an ingredient, additive, component or supplement, liquid or solid, suitable for incorporation in agricultural compositions.

The details of biological material used in the present invention:
Soy protein basically serves as plant-derived bio-stimulant which is readily available commercially. Traditionally, it is made from soybean meal that has been dehulled and defatted. Soy protein extract contains various amino acids – Aspartic, Threonine, Serine, Glutamic acid, Proline, Glycine, Valine, Cystine, Isoleucine, Methionine, Tyrosine, Phenylalanine, Lysine, Histidine, Arginine, and the percentage of each amino acid may vary in the mixture.

Increasing frequency of adverse environmental conditions for crop growth, have driven the development of bio stimulants. These are substances whose function is to stimulate nutrient uptake, nutrient use efficiency, and tolerance to abiotic stresses and crop quality. Soy protein has a bio stimulating effect on the health of the crops.

Soy protein functions as micronutrients and act synergistically along with the active ingredients in the composition in improving plant immune system. Soy protein improves the formulation, stability, and adhesion of the insecticide to plant surfaces and helps the insecticide spread more effectively and adhere to the plant, which can enhance its overall effectiveness. Further, the addition of Soy protein enhances protein synthesis in the meristematic tissues. The enhanced protein synthesis increases photosynthetic pigments in plants and that in turn results in accelerated photosynthetic reaction. Hence, the addition of Soy protein to the active ingredients additionally restores plant health.

The best results are however obtained when Soy protein is in the optimum range and such optimum range needs to be determined. Excess amount of Soy protein in the biological system will affect plant cells leading to adverse effects on the plant physiology, finally leading to phytotoxicity. Hence, the optimum range of Soy protein in the composition is very significant.

Soy Protein was procured from Sales Office 1900 Crown Colony 10 Drive, 3rd Floor, Quincy, Massachusetts, USA.

In one aspect, the present invention provides a novel, stable, synergistic, environmentally safe, insecticidal composition comprising:
Flonicamid in an amount ranging from 10-24% w/w;
Diafenthiuron in an amount ranging from 10-40%w/w;
Cyantraniliprole in an amount ranging from 2-20% w/w;
Soy protein in an amount ranging from 0.001-2%w/w; and
at least one agriculturally acceptable excipient.

In an embodiment of the present invention, Flonicamid is present in an amount ranging from 10-24% w/w.

In another embodiment of the present invention, Flonicamid is present in an amount ranging from 4-16% w/w.

In a preferred embodiment of the present invention, Flonicamid is present in amount of 7.30% w/w.

In an embodiment of the present invention, Diafenthiuron is present in an amount ranging from 10-40%w/w.

In another embodiment of the present invention, Diafenthiuron is present in an amount ranging from 20-35% w/w.

In a preferred embodiment of the present invention, Diafenthiuron is present in an amount of 22.5% w/w.

In an embodiment of the present invention, Cyantraniliprole is present an amount ranging from 2-20% w/w.

In another embodiment of the present invention, Cyantraniliprole is present in an amount ranging from 4-16% w/w.

In a preferred embodiment of the present invention, Cyantraniliprole is present is an amount of 5% w/w.

In an embodiment of the present invention, Soy protein is present in an amount ranging from 0.001-2% w/w.

In a preferred embodiment of the present invention, Soy protein is present in amount of 0.1% w/w.

In an embodiment of the present invention, said agriculturally acceptable excipient is selected from the group consisting of at least one anti-freeze agent, at least one dispersing agent, at least one wetting agent, at least one wetting cum dispersing agent, at least one antifoam, at least one anti-bacterial or biocide, at least one coating agent, at least one dye, at least one filler, at least one carrier, at least one thickener, and a combination thereof.

In a preferred embodiment of the present invention, said agriculturally acceptable excipient comprises a combination of at least an anti-freeze agent, wetting cum dispersing agent, antifoam, anti-bacterial or biocide, thickener and a combination thereof.

In an embodiment of the present invention, said at least one anti-freeze agent is selected from the group consisting of mono ethylene glycol, diethylene glycol, polypropylene glycol, ethylene alcohols, polyethylene glycols, glycerine, epichlorohydrin, urea, glycerol and sorbitol and combination thereof.

In a preferred embodiment of the present invention, said anti-freeze agent is propylene glycol.

In an embodiment of the present invention, said anti-freeze agent is present in amount ranging from 2-14% w/w.

In a preferred embodiment of the present invention, said anti-freeze agent is present in amount of 7% w/w.

In an embodiment of the present invention, said wetting cum dispersing agent is selected from a group consisting of alkyl phenol Ethoxylate, salts of alkyl naphthyl sulphonate, salts of alkyl aryl sulphonate, derivative of sulfonated fatty alcohol, anionic sulphonate, blend of aromatic sulphonic acid sodium salt, Blend of alkyl aryl sulphonic acid sodium salt and combination thereof.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is anionic sulphonate.

In an embodiment of the present invention, said wetting cum dispersing agent is present in an amount ranging from 2-10% w/w.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is present in amount of 7% w/w.

In an embodiment of the present invention, said antifoam is selected from the group consisting of silicone emulsions, long-chain alcohols, silicon based, fatty acids, organic fluorine compounds and combination thereof.

In a preferred embodiment of the present invention, said antifoam is silicon based.

In an embodiment of the present invention, said antifoam is present in an amount ranging from 0.2-3% w/w.
In a preferred embodiment of the present invention, said antifoam is present in amount of 0.5% w/w.

In an embodiment of the present invention, said anti-bacterial or biocide is selected from a group consisting of Clavulanic acid, Avibactam, stigmasterol, formaldehyde and combination thereof.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is formaldehyde.

In an embodiment of the present invention, said anti-bacterial or biocide is present in an amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is present in an amount of 0.2% w/w.

In an embodiment of the present invention, said thickener is selected from the group consisting of polyacrylamide, polyethylene polymers, polysaccharides (long-chain sugars), or vegetable oils and xanthan gum and combination thereof.

In a preferred embodiment of the present invention, said thickener is xanthan gum.

In an embodiment of the present invention, said thickener is present in amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said anti-freeze agent is present in amount of 7% w/w; said wetting cum dispersing agent is present in amount of 5% w/w; said antifoam is present in amount of 0.5% w/w; said anti-bacterial or biocide is present in amount of 0.2% w/w; and said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said insecticidal composition comprises
Flonicamid is present in an amount of 7.30% w/w;
Diafenthiuron is present in an amount of 22.5%w/w;
Cyantraniliprole is present in amount of 5% w/w;
Soy protein is present in amount of 0.1%w/w; and
at least one agriculturally acceptable excipient.

In an embodiment of the present invention, said insecticidal composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), wettable granules (WG), water-soluble granules (SG), wettable powders (WP), water-dispersible granule (WDG), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), dry flowables (DF), aqueous suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG).

In a preferred embodiment of the present invention, said insecticidal composition is in the form of Suspension concentrates (SC).

Suspension concentrates (SC) formulation has following advantages over other types of formulations:
SC formulations are generally more stable than other formulations. The active ingredients in SC are finely ground and suspended in a liquid, preventing settling and maintaining uniformity.
This stability ensures consistent efficacy over time and ease of storage without the need for constant agitation.
They are easier to handle and pour compared to powders, reducing the risk of inhalation by workers during mixing and application.
SC formulations typically have reduced risk of causing phytotoxicity compared to other formulations because they provide better coverage and are less concentrated. This can result in more even distribution and reduced risk of overdosing on plants.
SC formulations often provide better coverage and adhesion to plant surfaces due to their particle size and suspension properties. This can enhance the effectiveness of the active ingredients against pests or diseases.
They often have reduced environmental impact due to lower rates of active ingredients used and minimized risk of drift during application.
SC formulations tend to have longer shelf lives compared to other formulations, maintaining their efficacy for an extended period when stored properly.
Unlike emulsifiable concentrates that may require specific diluents or solvents for dilution, SC formulations often just require water for dilution before application, making them more straightforward to prepare.

The insecticidal composition of the present invention is Ready to Use Formulation (RTU). The ready to use formulation has various advantages over the tank mix of the active ingredients. These advantages include very few or no inhalation hazards, easy handling, low chances of sedimentation, being environmentally safer, good efficacy without phytotoxicity, lower transportation costs, and scientific practice as the formulation is prepared after stability and compatibility studies.

In another aspect, the present invention provides a process for the preparation of said insecticidal composition, comprising:
obtaining a homogeneous mixture of anti- freeze agent, wetting cum dispersing agent in D.M water;
adding antifoam, Flonicamid technical, Diafenthiuron technical and Cyantraniliprole technical to the homogenous mixture of step (a) to obtain a homogenous mass;
grinding said homogenous mass in sand mill until mean particle size of less than 5 microns is obtained;
adding Soy protein, biocide and 2% water suspension of a thickener under low stirring and mix well.

In an embodiment of the present invention, Flonicamid is present in an amount ranging from 10-24% w/w.

In another embodiment of the present invention, Flonicamid is present in an amount ranging from 8-16% w/w.

In a preferred embodiment of the present invention, Flonicamid is present in amount of 7.30% w/w.

In an embodiment of the present invention, Diafenthiuron is present in an amount ranging from 10-40%w/w.

In a preferred embodiment of the present invention, Diafenthiuron is present in an amount ranging from 20-35% w/w.

In a preferred embodiment of the present invention, Diafenthiuron is present in an amount of 22.5% w/w.

In an embodiment of the present invention, Cyantraniliprole is present an amount ranging from 2-20% w/w.

In another embodiment of the present invention, Cyantraniliprole is present in an amount ranging from 4-16% w/w.

In a preferred embodiment of the present invention, Cyantraniliprole is present is an amount of 5% w/w.

In an embodiment of the present invention, Soy protein is present in an amount ranging from 0.001-2% w/w.

In a preferred embodiment of the present invention, Soy protein is present in amount of 0.1% w/w.

In an embodiment of the present invention, said at least one anti- freeze agent is selected from the group consisting of mono ethylene glycol, diethylene glycol, polypropylene glycol, ethylene alcohols, polyethylene glycols, glycerine, epichlorohydrin, urea, glycerol and sorbitol and combination thereof.

In a preferred embodiment of the present invention, said anti- freeze agent is propylene glycol.

In an embodiment of the present invention, said anti-freeze agent is present in amount ranging from 2-14% w/w.

In a preferred embodiment of the present invention, said anti- freeze agent is present in amount of 7% w/w.

In an embodiment of the present invention, said wetting cum dispersing agent is selected from a group consisting of alkyl phenol Ethoxylate, salts of alkyl naphthyl sulphonate, salts of alkyl aryl sulphonate, derivative of sulfonated fatty alcohol, anionic sulphonate, blend of aromatic sulphonic acid sodium salt, Blend of alkyl aryl sulphonic acid sodium salt and combination thereof.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is anionic sulphonate.

In an embodiment of the present invention, said wetting cum dispersing agent is present in an amount ranging from 2-10% w/w.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is present in amount of 5% w/w.

In an embodiment of the present invention, said antifoam is selected from the group consisting of silicone emulsions, long-chain alcohols, silicon based, fatty acids, organic fluorine compounds and combination thereof.

In a preferred embodiment of the present invention, said antifoam is silicon based.

In an embodiment of the present invention, said antifoam is present in an amount ranging from 0.3-3% w/w.

In a preferred embodiment of the present invention, said antifoam is present in amount of 0.5% w/w.

In an embodiment of the present invention, said anti-bacterial or biocide is selected from a group consisting of Clavulanic acid, Avibactam, stigmasterol, formaldehyde and combination thereof.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is formaldehyde.

In an embodiment of the present invention, said anti-bacterial or biocide is present in an amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is present in an amount of 0.2% w/w.

In an embodiment of the present invention, said thickener is selected from the group consisting of polyacrylamide, polyethylene polymers, polysaccharides (long-chain sugars), or vegetable oils and xanthan gum and combination thereof.

In a preferred embodiment of the present invention, said thickener is xanthan gum.

In an embodiment of the present invention, said thickener is present in amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said anti-freeze agent is present in amount of 7% w/w; said wetting cum dispersing agent is present in amount of 5% w/w; said antifoam is present in amount of 0.5% w/w; said anti-bacterial or biocide is present in amount of 0.2% w/w; and said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said insecticidal composition comprises:
Flonicamid is present in an amount of 7.30% w/w;
Diafenthiuron is present in an amount of 22.5%w/w;
Cyantraniliprole is present in amount of 5% w/w;
Soy protein is present in amount of 0.1%w/w; and
at least one agriculturally acceptable excipient.

In yet another aspect, the present invention provides a method for combating pests in crops of useful plants without causing substantial harm to the crops, which comprises treating the useful plants, seeds or cuttings thereof or the locus of the useful plants with an insecticidally active amount of an insecticidal composition.

In an embodiment of the present invention, said insecticidal composition comprising:
Flonicamid in an amount ranging from 10-24% w/w;
Diafenthiuron in an amount ranging from 10-40%w/w;
Cyantraniliprole an amount ranging from 2-20% w/w;
Soy protein in an amount ranging from 0.001-2%w/w; and
at least one agriculturally acceptable excipient.

In an embodiment of the present invention, Flonicamid is present in an amount ranging from 10-24% w/w.

In another embodiment of the present invention, Flonicamid is present in an amount ranging from 8-16% w/w.

In a preferred embodiment of the present invention, Flonicamid is present in amount of 7.30% w/w.

In an embodiment of the present invention, Diafenthiuron is present in an amount ranging from 10-40%w/w.

In another embodiment of the present invention, Diafenthiuron is present in an amount ranging from 20-35% w/w.

In a preferred embodiment of the present invention, Diafenthiuron is present in an amount of 22.5% w/w.

In an embodiment of the present invention, Cyantraniliprole is present an amount ranging from 2-20% w/w.

In another embodiment of the present invention, Cyantraniliprole is present in an amount ranging from 4-16% w/w.

In a preferred embodiment of the present invention, Cyantraniliprole is present is an amount of 5% w/w.

In an embodiment of the present invention, Soy protein is present in an amount ranging from 0.001-2% w/w.

In a preferred embodiment of the present invention, Soy protein is present in amount of 0.1% w/w

In an embodiment of the present invention, said agriculturally acceptable excipient is selected from the group consisting of at least one anti-freeze agent, at least one dispersing agent, at least one wetting agent, at least one wetting cum dispersing agent, at least one antifoam, at least one anti-bacterial or biocide, at least one coating agent, at least one dye, at least one filler, at least one carrier, at least one thickener, and a combination thereof.

In a preferred embodiment of the present invention, said agriculturally acceptable excipient comprises a combination of at least an anti-freeze agent, wetting cum dispersing agent, antifoam, anti-bacterial or biocide, thickener and a combination thereof.

In an embodiment of the present invention, said at least one anti-freeze agent is selected from the group consisting of of mono ethylene glycol, diethylene glycol, polypropylene glycol, ethylene alcohols, polyethylene glycols, glycerine, epichlorohydrin, urea, glycerol and sorbitol and combination thereof.

In a preferred embodiment of the present invention, said anti-freeze agent is propylene glycol.

In an embodiment of the present invention, said anti-freeze agent is present in amount ranging from 2-14% w/w.
In a preferred embodiment of the present invention, said anti-freeze agent is present in amount of 7% w/w.

In an embodiment of the present invention, said wetting cum dispersing agent is selected from a group consisting of alkyl phenol Ethoxylate, salts of alkyl naphthyl sulphonate, salts of alkyl aryl sulphonate, derivative of sulfonated fatty alcohol, anionic sulphonate, blend of aromatic sulphonic acid sodium salt, Blend of alkyl aryl sulphonic acid sodium salt and combination thereof.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is anionic sulphonate.

In an embodiment of the present invention, said wetting cum dispersing agent is present in an amount ranging from 2-10% w/w.

In a preferred embodiment of the present invention, said wetting cum dispersing agent is present in the amount of 5% w/w.

In an embodiment of the present invention, said antifoam is selected from the group consisting of silicone emulsions, long-chain alcohols, silicon based, fatty acids, organic fluorine compounds and combination thereof.

In a preferred embodiment of the present invention, said antifoam is silicon based.

In an embodiment of the present invention, said antifoam is present in an amount ranging from 0.2-3% w/w.

In a preferred embodiment of the present invention, said antifoam is present in amount of 0.5% w/w.

In an embodiment of the present invention, said anti-bacterial or biocide is selected from a group consisting of Clavulanic acid, Avibactam, stigmasterol, formaldehyde and combination thereof.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is formaldehyde.

In an embodiment of the present invention, said anti-bacterial or biocide is present in an amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said anti-bacterial or biocide is present in an amount of 0.2% w/w.

In an embodiment of the present invention, said thickener is selected from the group consisting of polyacrylamide, polyethylene polymers, polysaccharides (long-chain sugars), or vegetable oils and xanthan gum and combination thereof.

In a preferred embodiment of the present invention, said thickener is xanthan gum.

In an embodiment of the present invention, said thickener is present in amount ranging from 0.08-5% w/w.

In a preferred embodiment of the present invention, said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said anti-freeze agent is present in amount of 7% w/w; said wetting cum dispersing agent is present in amount of 5% w/w; said antifoam is present in amount of 0.5% w/w; said anti-bacterial or biocide is present in amount of 0.2% w/w; and said thickener is present in amount of 0.3% w/w.

In a preferred embodiment of the present invention, said insecticidal composition comprises:
Flonicamid is present in an amount of 7.30% w/w;
Diafenthiuron is present in an amount of 22.5%w/w;
Cyantraniliprole is present in amount of 5% w/w;
Soy protein is present in amount of 0.1%w/w; and
at least one agriculturally acceptable excipient.

In an embodiment of the present invention, said insecticidal composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), wettable granules (WG), water-soluble granules (SG), wettable powders (WP), water-dispersible granule (WDG), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), dry flowables (DF), aqueous suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG).

In a preferred embodiment of the present invention, said insecticidal composition is in the form of Suspension concentrates (SC).

In an embodiment of the present invention, said insecticidal composition is applied at the dosage ranging from 800-1200g/ha to the crops.

In an embodiment of the present invention, said insecticidal composition can be used to control insects, pests and mites.

The major insects and pests against which the composition or formulation of the present invention can be used for controlling the same are those belonging to the order Hemiptera that may include rice leafhopper Nephotettix nigropictus, rice brown plant hopper Nilaparvata lugen, rice white backed plant hopper, Apple Mealy bug Phenococcus aceris, bean aphid Aphis fabae, black citrus aphid Toxoptera aurantii, citrus black scale Saissetia oleae, cabbage aphid Brevicoryne brassicae, Lipaphis erysimi, citrus red scale Aonidiella aurantii, yellow scale Aonidiella citrine, citrus mealybug Planococcus citri, corn leaf aphid Rhopalosiphum maidis, cotton aphid Aphis gossypii, cotton jassid Amrasca biguttula biguttla, cotton mealy bug Planococcus spp. and Pseudococcus spp., cotton stainer Dysdercus suturellus, cotton whitefly Bemisia tabaci, cowpea aphid Aphis crassivora, grain aphid Sitobion avenae, golden glow aphid Uroleucon spp., grape mealybug Pseudococcus maritimus, green peach aphid Myzus persicae, greenhouse whitefly Trialeurodes vaporariorum, papaya mealy bug Pracoccus marginatus, pea aphid Acyrthosiphon pisum, sugarcane mealybug Saccharicoccus sacchari, potato aphid Myzus persicae, potato leaf hopper Empoasca fabae, cotton whitefly Bemisia tabaci, tarnished plant bug Lygus lineolaris, wooly apple aphid Eriosoma lanigerum, mango hopper Amritodus atkinsoni, Idioscopus spp.; order Lepidoptera, that may include army worm Mythimna unipuncta, asiatic rice borer Chilo suppressalis, bean pod borer Maruca vitrata, beet armyworm Spodoptera exigua, black cutworm Agrotis ipsilon, bollworm Helicoverpa armigera, cabbage looper Trichoplusia ni, codling moth Cydia pomonella, croton caterpillar Achea janata, diamond backmoth Plutella xylostella, cabbage worm Pieris rapae, pink bollworm Pectinophora gossypiella, sugarcane borer Diatraea saccharalis, tobacco budworm Heliothis virescens, tomato fruitworm Helicoverpa zea, velvet bean caterpillar Anticarsia gemmatalis, yellow stem borer Scirpophaga incertulas, spotted bollworm Earias vittella, rice leaf folder Cnaphalocrocis medinalis, pink stem borer Sesamia spp., order Thysanoptera that may include Frankliniella spp., Thrips palmi, Thrips tabaci and Scirtothrips dorsali or the like.

The insecticidal composition of the present invention may be effectively used for controlling insects, pests and mites infesting various crops including but not limited to cereals, cotton, pulses, oilseeds, fibre crop, sugar crops, leafy vegetables, chili, okra, tuber crops, fruit crops; or other plants for example flowering or ornamentals plants.

In an embodiment, the Insecticidal composition of the present invention comprising said actives along with Soy protein exhibits synergistic effect in control of Thrips, Jassids and White fly pests in agricultural crops, particularly in cotton.

The present invention provides an insecticidal composition comprising said combination that possesses a broad-spectrum insecticide with enhanced efficacy at reduced cumulative dosage as compared to solo ingredient compounds when applied individually.

EXAMPLES
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.

Example 1: Preparation of insecticidal composition as suspension concentrates (SC).

Table no. 1 below provides an insecticidal composition as described in the present invention, formulated as suspension concentrates (SC).

Table 1: Chemical Composition of CI 73251 SC

Ingredients Tentative percentage w/w
Flonicamid technical (Basis of 100.0%) 7.30% w/w
Diafenthiuron technical (Basis of 100.0%) 22.5% w/w
Cyantraniliprole technical (Basis of 100.0%) 5.0% w/w
Soy protein 0.1% w/w
Anti-freeze agent (Propylene glycol) 7.0% w/w
Wetting cum dispersing agent (anionic sulphonate) 5% w/w
Antifoam (silicon based) 0.5% w/w
Anti-bacterial or biocide 0.2% w/w
Thickener (xanthan gum) 0.3% w/w
D.M Water QS to 100%

Table 2: Quality parameters of GR formulation
S. No. Parameter Desired quality
1 Description the material shall be in the form of white to off white color viscous suspension free from extraneous matter.
2 Active ingredient Content Flonicamid: 7.3% (+10, -5%)
Diafenthiuron: 22.5% (+10, -5%)
Cyantraniliprole: 5% (+10 ,5%,)
3 Persistent foam after 1 minute Max. 60ml
4 Suspensibility Min. 80%
5 pH of 1% aq. Solution 6-9
6 Wet sieve test through 45
microns Min. 99%
7 Pourability “Residue” Max. 6.0%

Example 2: Field evaluation of the bio efficacy of the present herbicidal composition:

The presently disclosed insecticidal composition (coded as CI 73251 SC) was tested for its bio efficacy against Thrips, Jassids and White fly on cotton crop in a local variety during Kharif season 2023 in Maharashtra. The plants were aligned in the plot size of 50 sq meter. The trial was laid out in a random block design consisting of a total of 10 treatments in three replications. The trial was conducted at a temperature of 37ºC under 80% relative humidity, no winds and under optimum soil moisture conditions.

Measured quantity of the chemical was added to required volume of water @ 375 lit. /Ha for spray. The spray tank was filled with ½ the quantity of clean required volume of water and then the measured chemical (according to the dose) was added followed by the rest half quantity of water. The solution was stirred well before application. A Knapsack sprayer fitted with boom along with flood jet nozzle was used to apply the insecticide solution.

Table 3 below provides the treatment details in the trial experiment conducted. For purposes of convenience, the present composition has been represented by the code “CI 73251 SC.”

Treatments Product a.i./Ha Dose/Ha
T-1 Untreated - -
T-2 CI-7325 SC 58.4 + 180 + 40 800
T-3 CI-7325 SC 73 + 225 + 50 1000
T-4 CI-7325 SC 87.6 + 270 + 60 1200
T-5 CI-73251 SC 58.4 + 180 + 40 + 8 800
T-6 CI-73251 SC 73 + 225 + 50 + 10 1000
T-7 CI-73251 SC 87.6 + 270 + 60 + 12 1200
T-8 Cyantraniliprole 10.26 %OD 90 900
T-9 Flonicamid 50 % WG 75 150
T-10 Diafenthiuron50% WP 300 600

CI-7325 SC Flonicamid 7.3 % + Diafenthiuron22.5 % + Cyantraniliprole 5 % SC
CI-73251 SC Flonicamid 7.3 % + Diafenthiuron22.5 % + Cyantraniliprole 5 % + Soy Protein 0.1% SC

EVALUATION OF BIO-EFFICACY AGAINST PESTS MANAGEMENT
Insects’ population count: After 3, 7 and 10 days of first and second applications, insects were counted from three leaves of 10 tagged plants.

Percent Insect Control =(IC-IT)/IC* IC
Where
IC= No. of Insects in control plot
IT= No. of Insects in treated plot
Table- 3 (a): Effect of Insecticide treatment on White fly at 3, 7 & 10 days after Application
Treatments Product a.i./ Ha Dose/Ha 3DA A %Control 7 DA A %Control 10DAA % Control
T-1 Untreated - 17 0 21.5 0 26.3 0
T-2 CI-7325 SC 58.4 +180 + 40 800 4.5 73.53 5.2 75.81 7.8 70.34
T-3 CI-7325 SC 73 + 225 + 50 1000 2.5 85.29 2.9 86.51 4.2 84.03
T-4 CI-7325 SC 87.6 + 270 + 60 1200 2.3 86.47 2.7 87.44 4.1 84.41
T-5 CI-73251 SC 58.4 + 180 40 + 8 800 2.5 85.29 2.6 87.91 3.8 85.55
T-6 CI-73251 SC 73 +225 + 50 + 10 1000 1.8 89.41 2.1 90.23 3.5 86.69
T-7 CI-73251 SC 87.6+ 270+ 60+ 12 1200 1.7 90.00 2 90.70 3.6 86.31
T-8 Cyantraniliprole 10.26 % OD 90 900 6.5 61.76 5.5 74.42 7.5 71.48
T-9 Flonicamid 50 % WG 75 150 5.4 68.24 6.5 69.77 6.8 74.14
T-10 Diafenthiuron 50% WP 300 600 3.2 81.18 4.2 80.47 6.5 75.29

As seen in Table 3a, treatment with Flonicamid alone at 75 g a.i/ha results in about 68% control of pests after first application. Diafenthiuron alone at 300 g a.i/ha results in about 81% control of pests after first application. Treatment with Cyantraniliprole alone at 90g/ha results in 61% control of pests after first application. However, unexpectedly and surprisingly, advantageously, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 89% control of pests after first application. It can be observed that when the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein is applied, it synergistically enhances the overall activity of the insecticidal composition. The results obtained were superior to other possible combinations at different weight concentrations.

Treatment with the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha results in about 85% control of pests after first application. In contrast, when Soy protein is added to the combination, unexpectedly and surprisingly, the efficacy of the combination Flonicamid + Diafenthiuron+ Cyantraniliprole is enhanced. As seen in the table, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 89% control of pests after first application. These data clearly establish that Soy protein is able to enhance the efficacy of the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole in a synergistic manner, which is unexpected and surprising.

Table- 3 (b): Effect of Insecticide treatment on Jassids at 3, 7 & 10 days after Application
Treatments Product a.i./Ha Dose/ Ha 3DA A %Contr ol 7DA A %Control 10 DAA %Control
T-1 Untreated - - 11 0 15.3 - 17.2 -
T-2 CI-7325 SC 58.4 +180 + 40 800 3.4 69.09 3.5 77.12 3.8 77.91
T-3 CI-7325 SC 73 + 225+50 1000 2.5 77.27 2.8 81.70 3 82.56
T-4 CI-7325 SC 87.6 + 270 + 60 1200 2.3 79.09 2.4 84.31 2.6 84.88
T-5 CI-73251 SC 58.4 +180 +40 +8 800 2.4 78.18 2.7 82.35 2.9 83.14
T-6 CI-73251 SC 73 + 225 + 50 + 10 1000 1.8 83.64 1.9 87.58 2 88.37
T-7 CI-73251 SC 87.6 + 270 + 60 + 12 1200 1.7 84.55 1.8 88.24 1.9 88.95
T-8 Cyantraniliprole 10.26 % OD 90 900 2.5 77.27 2.7 82.35 3.1 81.98
T-9 Flonicamid
50 % WG 75 150 2.8 74.55 3 80.39 3.4 80.23
T-10 Diafenthiuron 50% WP 300 600 3.4 69.09 3.5 77.12 3.7 78.49

As seen in Table 3b, treatment with Flonicamid alone at 75 g a.i/ha results in about 74% control of pests after first application. Diafenthiuron alone at 300 g a.i/ha results in about 69% control of pests after first application. Treatment with Cyantraniliprole alone at 90g/ha results in 77% control of pests after first application. However, unexpectedly and surprisingly, advantageously, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 84% control of pests after first application. It can be observed that when the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein is applied, it synergistically enhances the overall activity of the insecticidal composition. The results obtained were superior to other possible combinations at different weight concentrations.

Treatment with the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha results in about 77% control of pests after first application. In contrast, when Soy protein is added to the combination, unexpectedly and surprisingly, the efficacy of the combination Flonicamid + Diafenthiuron+ Cyantraniliprole is enhanced. As seen in the table, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 84% control of pests after first application. These data clearly establish that Soy protein is able to enhance the efficacy of the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole in a synergistic manner, which is unexpected and surprising.

Table- 3 (c): Effect of Insecticide treatment on Thrips at 3, 7 & 10 days after Application

Tretments Product a.i./Ha Dose
/Ha 3 DA A % Control 7 DA A % Control 10 DAA % Control
T-1 Untreated - - 14.3 - 16.2 - 18.3 -
T-2 CI-7325 SC 58.4 + 80+ 40 800 2.7 81.12 2.9 82.10 4 78.14
T-3 CI-7325 SC 73 + 225 +50 1000 2.1 85.31 2.7 83.33 3.5 80.87
T-4 CI-7325 SC 87.6 + 70+ 60 1200 0.9 93.71 2.6 83.95 3.2 82.51
T-5 CI-73251 SC 58.4 + 180 + 40 + 8 800 1.8 87.41 1.4 91.36 2.7 85.25
T-6 CI-73251 SC 73 + 225 + 50 + 10 1000 0.7 95.10 1 93.83 2.1 88.52
T-7 CI-73251 SC 87.6 + 270 + 60 + 12 1200 0.6 95.80 0.9 94.44 1.8 90.16
T-8 Cyantranili prole 10.26 % OD 90 900 2.5 82.52 2.7 83.33 3.5 80.87
T-9 Flonicamid 50 % WG 75 150 7.5 47.55 8 50.62 9 50.82
T-10 Diafenthiuron 50% WP 300 600 8.3 41.96 8.5 47.53 10.2 44.26

As seen in Table 3c, treatment with Flonicamid alone at 75 g a.i/ha results in about 47% control of pests after first application. Diafenthiuron alone at 300 g a.i/ha results in about 41% control of pests after first application. Treatment with Cyantraniliprole alone at 90g/ha results in 82% control of pests after first application. However, unexpectedly and surprisingly, advantageously, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 95% control of pests after first application. It can be observed that when the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein is applied, it synergistically enhances the overall activity of the insecticidal composition. The results obtained were superior to other possible combinations at different weight concentrations.

Treatment with the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha results in about 85% control of pests after first application. In contrast, when Soy protein is added to the combination, unexpectedly and surprisingly, the efficacy of the combination Flonicamid + Diafenthiuron+ Cyantraniliprole is enhanced. As seen in the table, the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy protein at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g ai/ha respectively results in about 95% control of pests after first application. These data clearly establish that Soy protein is able to enhance the efficacy of the combination of Flonicamid + Diafenthiuron+ Cyantraniliprole in a synergistic manner, which is unexpected and surprising.
Example 3: Evaluation of Phytotoxicity

Visual observations were recorded at 3, 7 and 10 days after application (DAA) of testing products. The parameters were observed leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. The score scale (1-10) followed for leaf injury on tips/surface is given.

Table 4: Phytotoxicity symptoms scoring and rating for leaf injury on tip/surface.

Leaf injury on tips/surface Rating
0-10 % 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

Phytotoxicity studies
Table- 4 (a): Phytotoxic effect of various treatments on Cotton crop after 3 DAA
Treatments Product a.i./Ha Dose/Ha L N V E H W
T-1 Untreated - 0 0 0 0 0 0
T-2 CI-7325 SC 58.4 + 180 + 40 800 0 0 0 0 0 0
T-3 CI-7325 SC 73 + 225 +50 1000 0 0 0 0 0 0
T-4 CI-7325 SC 87.6 + 270 +60 1200 0 0 0 0 0 0
T-5 CI-73251 SC 58.4 + 180 + 40 + 8 800 0 0 0 0 0 0
T-6 CI-73251 SC 73 + 225 +50 + 10 1000 0 0 0 0 0 0
T-7 CI-73251 SC 87.6 + 270 + 60 + 12 1200 0 0 0 0 0 0
T-8 Cyantraniliprole
10.26 % OD 90 900 0 0 0 0 0 0
T-9 Flonicamid 50 %
WG 75 150 0 0 0 0 0 0
T-10 Diafenthiuron50%
WP 300 600 0 0 0 0 0 0
DAA – Days after application,
L- Leaf injury on tips/surface, N-Necrosis, V- Vein clearing, E- Epinasty, H- Hyponasty, W- wilting.

Table- 4 (b) Phytotoxic effect of various treatments on Cotton crop after 7 DAA

Treatments Product a.i./Ha Dose/Ha L N V E H W
T-1 Untreated - - 0 0 0 0 0 0
T-2 CI-7325 SC 58.4 + 180 +40 800 0 0 0 0 0 0
T-3 CI-7325 SC 73 + 225 +50 1000 0 0 0 0 0
T-4 CI-7325 SC 87.6 + 270 +60 1200 0 0 0 0 0 0
T-5 CI-73251 SC 58.4 + 180 +40 + 8 800 0 0 0 0 0 0
T-6 CI-73251 SC 73 + 225 +50 + 10 1000 0 0 0 0 0 0
T-7 CI-73251 SC 87.6 + 270 +60 + 12 1200 0 0 0 0 0 0
T-8 Cyantraniliprole
10.26 % OD 90 900 0 0 0 0 0 0
T-9 Flonicamid 50 %
WG 75 150 0 0 0 0 0 0
T-10 Diafenthiuron50%
WP 300 600 0 0 0 0 0 0

DAA – Days after application,
L- Leaf injury on tips/surface, N-Necrosis, V- Vein clearing, E- Epinasty, H- Hyponasty, W-wilting.

As seen in Tables 4(a) and 4(b), there is no phytotoxicity associated with treatment of any of the ingredients to the plants, suggesting that said composition is completely safe to use. The present invention provides a novel and synergistic combination of Flonicamid + Diafenthiuron+ Cyantraniliprole + Soy proteins, which significantly enhances the economic value of crops. While the present specification illustrates various concentrations across which enhanced results are seen, in particular, the combination of Flonicamid + Diafenthiuron + Cyantraniliprole + Soy proteins at concentration of 73 g a.i/ha + 225 g a.i/ha + 50 g a.i/ha + 10 g a.i/ha respectively shows the best results in controlling pests. There is a clear and significant enhancement which is not anticipated or expected.

From the foregoing it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.
,CLAIMS:We Claim:

1. An insecticidal composition comprising:
a) Flonicamid in an amount ranging from 5-20% w/w;
b) Diafenthiuron in an amount ranging from 10-40%w/w;
c) Cyantraniliprole in an amount ranging from 2-20% w/w;
d) Soy protein in an amount ranging from 0.001-2%w/w; and
e) at least one agriculturally acceptable excipient in an amount ranging from 5-50% w/w.

2. The insecticidal composition as claimed in claim 1, wherein at least one agriculturally acceptable excipient is selected from anti-freezing agent, wetting cum dispersing agent, antifoam, anti-bacterial or biocide, thickener and combination thereof.

3. The insecticidal composition as claimed in claim 2, wherein said insecticidal composition comprises at least an anti-freezing agent ranging from 2-14%w/w, at least a wetting cum dispersing agent ranging from 2-10%w/w, at least an antifoam ranging from 0.2-3% w/w, at least an anti-bacterial or biocide ranging from 0.08-5%w/w and at least a thickener ranging from 0.08-5%w/w of the insecticidal composition.

4. The insecticidal composition as claimed in claim 1, wherein Flonicamid is present in an amount of 7.3% w/w; Diafenthiuron is present in an amount of 22.5% w/w; Cyantraniliprole is present in amount of 5% w/w and Soy protein is present in an amount of 0.1% w/w.

5. The insecticidal composition as claimed in claim 3, wherein said insecticidal composition comprises at least an anti-freezing agent is present in an amount of 7%w/w, at least a wetting cum dispersing agent is present in amount of 5%w/w, at least an antifoam is present in an amount of 0.5% w/w, at least an anti-bacterial or biocide is present in amount of 0.2%w/w and at least a thickener is present in amount of 0.3%w/w of the insecticidal composition.

6. The insecticidal composition as claimed in claim 1, wherein said insecticidal composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-dispersion (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), dry flowables (DF), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), aqueous Suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG).

7. The insecticidal composition as claimed in claim 6, wherein said insecticidal composition is preferably formulated in the form of Suspension concentrates (SC).

8. A process for preparing the insecticidal composition claimed in claim 7 as suspension concentrates formulation, said process comprising:
a) obtaining a homogeneous mixture of anti-freezing agent, wetting-cum-dispersing agent in D.M water;
b) adding antifoam, Flonicamid technical, Diafenthiuron technical and cyantraniliprole technical to the homogenous mixture of step (a) to obtain a homogenous mass;
c) grinding said homogenous mass in sand mill until a mean particle size of less than 5 microns is obtained;
d) adding soy protein, biocide and 2% water suspension of a thickener under low stirring and mixing well.

9. The process as claimed in claim 8, wherein a) Flonicamid is present in an amount ranging from 5-20% w/w; b) Diafenthiuron is present in an amount ranging from 10-40%w/w; c) Cyantraniliprole is present in an amount ranging from 2-20% w/w and d) Soy protein is present in an amount ranging from 0.001-2% w/w.

10. The process as claimed in claim 8, wherein said insecticidal composition comprises at least an anti-freezing agent is present in an amount of 7%w/w, at least a wetting cum dispersing agent in amount of 5%w/w, at least an antifoam agent is present in an amount of 0.5% w/w, at least an anti-bacterial or biocide is present in an amount of 0.2%w/w and at least a thickener is present in an amount of 0.3%w/w of the insecticidal composition.

11. A method for combating pests in crops of useful plants without causing substantial harm to the crops, which comprises treating the useful plants, seeds or cuttings thereof or the locus of the useful plants with an insecticidally active amount of an insecticidal composition.

12. The method as claimed in claim 11, wherein said insecticidal composition comprises:
a) Flonicamid in an amount ranging from 5-20% w/w;
b) Diafenthiuron in an amount ranging from 10-40%w/w;
c) Cyantraniliprole in an amount ranging from 2-20% w/w;
d) Soy protein in an amount ranging from 0.001-2%w/w; and
e) at least one agriculturally acceptable excipient in an amount ranging from 5-50% w/w.
13. The method as claimed in claim 12, wherein at least one agriculturally acceptable excipient is selected from anti-freezing agent, wetting cum dispersing agent, antifoam, anti-bacterial or biocide, thickener and combination thereof.

14. The method as claimed in claim 13, wherein said insecticidal composition comprises at least an anti-freezing agent is present in an amount of 7%w/w, at least a wetting cum dispersing agent is present in amount of 5%w/w, at least a antifoam is present in an amount of 0.5% w/w, at least an anti-bacterial or biocide is present in amount of 0.2%w/w and at least a thickener is present in amount of 0.3%w/w of the insecticidal composition.

15. The method as claimed in claim 12, wherein said insecticidal composition is formulated as Suspension concentrates (SC).

16. The method as claimed 11, wherein said insecticidal composition is applied at the dosage of 1000g/ha to the crops.