Description:FIELD OF THE INVENTION
The present invention relates to the field of pesticides. The present invention in particular relates to a synergistic, broad spectrum insecticidal composition comprising Cyclaniliprole, Bifenthrin and Diafenthiuron. The present invention further relates to the process of preparation of said composition and uses thereof.
BACKGROUND OF THE INVENTION
The background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
Enhancement of agricultural produce requires the protection of the crops and its produce from pest damage. Various chemicals and their formulations have been developed and are in use currently for the effective management of insects and pests. Due to non-judicious use of the hitherto known pesticides, the pests gain resistance and become hard to kill. Physically compatible pesticide mixtures exhibit a better pest management. These mixtures show multifaceted advantages than when applied individually, providing a synergistic effect.
The need for more food has to be met through higher yields per unit of land, water, energy and time. Excessive use of mineral fertilizers and chemical pesticides has caused soil degradation, ground water pollution and the spread of the pest's resistant to pesticides in several areas. Hence their judicious use includes avoiding prophylactic sprays, adopting strip treatment, spot application to only those areas with heavy incidence of pests, application to the soil to avoid direct contact with the natural enemies and using selective or non-persistent pesticides. The systemic pesticides are sprayed at a concentration of 0.02 to 0.05 percent active ingredient. The contact pesticides are sprayed at 0.05 to 0.07 or even 0.1 percent active ingredient. The soil application of the granular systemic insecticides varies from 1 to 2 kg a.i./ha. The fungicides are applied up to 2 g/l depending upon the chemical used, pest species and season of the application.
Processes for insecticidal agents and compositions have been developed to control insect pests and in practice have been used as a single or a mixed agent. However, processes for the economically efficient and ecologically safe insect control compositions are still being sought. A process for the preparation of insecticidal compositions which allows for reduced effective dosage rates, increased environmental safety and lower incidence of insect resistance are highly desirable. Although the rotational application of insect control agents having different modes of action may be adopted for good pest management practice, this approach does not necessarily give satisfactory insect control. Further, even though combinations of insect control agents have been studied, a high synergistic action has not always been found. Obtaining an insecticidal composition which demonstrates no cross-resistance to existing insecticidal agents, no toxicity problems and little negative impact on the environment is extremely difficult.
Cyclaniliprole with CAS registration number 1031756-98-5is a new anthranilic diamide insecticide proposed for agricultural use. Like other diamide insecticides such as chlorantraniliprole and cyantraniliprole, cyclaniliprole is a ryanodine receptor modulatorand known to impair nerve and muscle function which leads to rapid feeding cessation, regurgitation, lethargy and contractile paralysis of insects.
It has an IUPAC name 2’,3-dibromo-4'-chloro-1-(3-chloro-2-pyridyl)-6'-{[(1RS)-1-cyclopropylethyl]carbamoyl}pyrazole-5-carboxanilide]having chemical structure as:

Cyclaniliprole
Diafenthiuron with CAS registration number 80060-09-9, is an aromatic ether that is 1,3-diisopropyl-5-phenoxybenzene it is used to control mites, aphids and whitefly in cotton. It has a role as an oxidative phosphorylation inhibitor and an insecticide. It is a thiourea acaricide, a thiourea insecticide and an aromatic ether.
It has an IUPAC name 1-tert-butyl-3-[4-phenoxy-2,6-di(propan-2-yl)phenyl]thiourea having chemical structure as:

DIAFENTHIURON
Bifenthrinwith CAS registration number 82657-04-3,is a pyrethroid insecticide. It is a contact insecticide, and it acts by disrupting the gating mechanism of sodium channels that are involved in the generation and conduction of nerve impulses, causing rapid paralysis and death of the insects. Bifenthrin controls insect species such as Coleoptera, Diptera, Heteroptera, Lepidoptera, Orthoptera and some Acarina.
It has an IUPAC name(2-methylbiphenyl-3yl-methyl-(Z)-(1RS, 3RS)-3-(2-chloro-3,3,3-trifluoroprop-1-enyl]-2,2-dimethylcyclopropanecarboxylate.

BIFENTHRIN
The existing problems and limitations in the current agricultural pest management techniques that the present disclosure seeks to address:
Development of Pest Resistance:
Many pests have developed resistance to single active ingredient insecticides, making them less effective over time.
Conventional insecticides often require increased dosages to achieve the same level of pest control, which is unsustainable and increases the risk of environmental contamination.
The frequent use of single-action insecticides leads to the rapid evolution of resistant pest populations, reducing long-term pest management efficacy.
Limited Spectrum of Pest Control:
Single active ingredient insecticides or basic tank mixtures may not be effective against a broad range of insect pests, leading to incomplete control.
Farmers often need to use multiple products to target different pests, which increases costs and complicates pest management practices.
Existing solutions may fail to control certain pests like whiteflies, thrips, aphids, and mites effectively in crops like cotton and chili.
Suboptimal Synergy in Tank Mixes:
Tank mixtures of individual insecticides do not always exhibit true synergistic effects. The combination may result in antagonistic or merely additive effects, which do not significantly enhance pest control.
Inconsistent performance of tank mixes can be attributed to the instability of the combined components, leading to inadequate pest control and uneven application.
Stability Issues in Formulations:
Existing insecticidal formulations often suffer from stability issues, especially under varying environmental conditions such as high or low temperatures.
Instability can lead to the separation of active ingredients, reduced efficacy, and challenges in storage and transport.
Unstable formulations are harder to apply uniformly, affecting the overall effectiveness of pest control measures.
Need for Higher Dosages:
Due to the lack of synergistic action, traditional formulations or single active ingredient products may require higher dosages to achieve effective pest control.
Increased dosages not only raise the cost of pest management but also heighten the risk of crop phytotoxicity and environmental damage, including potential harm to beneficial insects and non-target organisms.
Environmental and Safety Concerns:
The use of higher concentrations of insecticides contributes to environmental pollution and poses health risks to farmworkers and consumers.
There is a growing need for formulations that are effective at lower concentrations, minimizing environmental impact while maintaining pest control efficacy.
Safety concerns also arise from formulations that produce harmful residues or have poor degradability, necessitating safer and more eco-friendly options.
Application Challenges and Inconsistent Results:
Some existing formulations are difficult to mix or apply, leading to inconsistent distribution of the insecticide on crops.
Poorly mixed insecticides may form clumps or separate into different phases, making uniform application challenging and reducing the effectiveness of the treatment.
The variability in application quality can result in areas of the crop being under protected, leaving them vulnerable to pest damage.
Economic Inefficiency:
The need for multiple insecticides or repeated applications due to inadequate control increases the financial burden on farmers.
Inefficient pest management practices can lead to significant crop losses and reduced profitability for farmers.
A more effective, stable, and broad-spectrum insecticidal composition can reduce overall costs and enhance agricultural productivity.
The present disclosure aims to overcome these limitations by providing a synergistic insecticidal composition of Cyclaniliprole, Bifenthrin and Diafenthiuron,, ensuring high efficacy at lower dosages, broad-spectrum pest control, formulation stability, and minimal environmental impact.
OBJECT OF THE INVENTION
The main objective of the disclosure is to provide a synergistic insecticidal composition combining Cyclaniliprole, Bifenthrin and Diafenthiuronthat enhances pest control efficacy beyond the sum of individual effects.
Another important object of the present disclosure is to deliver broad-spectrum pest control that effectively targets a variety of insect pests, including Fruit borer, whitefly, Bollworm &Jassid across different crops like Brinjal and cotton.
Another object of the present disclosure is to reduce the likelihood of pest resistance by utilizing a multi-component approach, thereby decreasing the dependence on single-action insecticides.
Yet another object of the present disclosure is to ensure stability of the formulation under various environmental conditions, minimizing separation or degradation of active ingredients for consistent performance.
Yet another object of the present disclosure is to lower the required dosage levels while maintaining effective pest control, reducing potential phytotoxicity to crops and minimizing environmental impact.
Yet another object of the present disclosure is to provide an easy-to-mix and apply formulation that ensures uniform distribution on crops, improving application efficiency and overall pest control results.
Yet another object of the present disclosure is to create an environmentally safer alternative by minimizing harmful residues, thus reducing health risks for farmers, consumers, and non-target organisms.
SUMMARY OF THE INVENTION
Accordingly, in one aspect, the present invention provides an insecticide composition comprising Cyclaniliprole, Bifenthrin and Diafenthiuron.
In one aspect, the present invention provides a synergistic composition of Cyclaniliprole, Bifenthrin and Diafenthiuron and agrochemically acceptable additives.
In yet another aspect, the present invention provides a synergistic composition comprising Cyclaniliprole, Bifenthrin and Diafenthiuron, the composition possesses Insecticidal activity.
In a further aspect, the present invention provides a method for effective control of various pest in plants.
In one aspect of the present invention, the Insecticidal composition of the present invention further comprises an agrochemically acceptable excipients selected from the group comprising an emulsifier; dispersing agent; wetting agent; antifoaming agent; rheology modifier; solvent; pH modifier; inert carrier; biocide; an antifreezing agent; Rheology Modifier; pH Stabiliizers; Wetting cum dispersing agent Binding agents ;stabilizing agent; and coloring agent.
In another aspect of the present invention, the Insecticidal composition is formulated as capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG) Emulsifiable water-in-oil (EO), Emulsifiable powder (EP), Emulsifiable for seed treatment (ES), Emulsifiable oil-in-water (EW), flowable concentrate for seed treatment (FS), Suspension Concentrate (SC), Suspo-emulsion (SE), Oil Dispersion(OD).Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG) and Wettable powders (WP), a mixed formulation of CS and SC (ZC), soluble liquid (SL).
In another further aspect the insecticidal composition of the present invention is preferably formulated as a Water dispersible granules (WDG) , Wettable powders (WP) , Suspension Concentrate (SC),flowable concentrate for seed treatment (FS), and Oil Dispersion (OD).
In yet another embodiment of the present invention, the invention further provides the process for preparation of the said formulation wherein, the said formulation can be one or more of capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG) Emulsifiable water-in-oil (EO), Emulsifiable powder (EP), Emulsifiable for seed treatment (ES), Emulsifiable oil-in-water (EW), flowable concentrate for seed treatment (FS), Suspension Concentrate (SC), Suspo-emulsion (SE), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG) and Wettable powders (WP), a mixed formulation of CS and SC (ZC), soluble liquid (SL).
DETAILED DESCRIPTION OF THE INVENTION
Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods. The illustrative examples are described in this section in connection with the embodiments and methods provided. The invention according to its various aspects is particularly pointed out and distinctly claimed in the appended claims read in view of this specification and appropriate equivalents.
It is to be noted that, as used in the specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
The expression of various quantities in terms of “% w/w” or “%” means the percentage by weight, relative to the weight of the total solution or composition unless otherwise specified.
The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control of Fungal-pests or disease.
As used herein, the terms "comprises", "comprising", "includes", "including", or any other variation thereof, are intended to cover a non-exclusive inclusion, subject to any limitation explicitly indicated. For example, a composition or a method that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, or method.
The term "synergistic", as used herein, refers the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.
As used herein, the term “composition” or "formulation" can be used interchangeably, unless stated otherwise, is meant to encompass, and are not limited to, compositions or formulations containing the combination of Cyantraniliprole, Bifenthrin and Diafenthiuron.
As used herein, the term “additive(s)” or "auxiliary agent(s)" or “agrochemically acceptable carrier(s)” can be used interchangeably and refers to inert substances which are commonly used as diluent, to provide stability or to increase the activity profile of the composition or formulation with or without having agrochemical activity or direct effect on the undesired phytopathogenic insects and/or microorganisms.
As used herein, the term "agrochemically acceptable salts" are typically acid addition salts of inorganic or organic acids, preferably of hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, perchloric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, malonic acid, toluenesulfonic acid or benzoic acid.
As used herein, the term "effective amount" means the amount of the active substances in the compositions to achieve an observable effect on growth, including the effects of necrosis, death,retardation, prevention, and removal, destruction, or otherwise diminishing the occurrence and activity of the target organism. The effective amount can vary for the various compositions used in the present invention. An effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like.
In an embodiment, the Insecticidal composition wherein the Cyclaniliprole ranging from 0.1% to 25.0% by weight of the Insecticidal composition.
In an embodiment, the Insecticidal composition wherein the Bifenthrin ranging from 0.1% to 25.0% by weight of the Insecticidal composition.
In an embodiment, the Insecticidal composition wherein the Diafenthiuron ranging from 0.1% to 50.0% by weight of the Insecticidal composition.
In another embodiment of the present invention, the invention further provides the process for preparation of the said formulation wherein, the said formulation can be one or more of as suspension concentrate (SC), wettable granules (WG), wettable powder (WP), a water dispersible granule (WDG), a water dispersible tablet (WT), an ultra-low volu (ULV) liquid (UL), an ultra-low volume (ULV) suspension (SU), a water soluble powder (SP), a suspo-emulsion (SE), granule (GR), ,flowable concentrate for seed treatment (FS), an emulsifiable granule (EG), an oil-in-water or water in oil emulsion (EW), an emulsifiable concentrate (EC), a micro-emulsion (ME), an oil dispersion (OD), a capsule suspension (CS), a dustable powder (DP) or an aerosol (AE).
In preferred embodiment of the present invention, the formulation is Suspension Concentrate (SC), oil dispersion (OD), ,flowable concentrate for seed treatment (FS),Water dispersible granules (WDG), Granules (GR) and Wettable powders (WP).
In yet another embodiment of the present invention, the agrochemically acceptable excipients of the formulation are selected from the group consisting of Emulsifiers dispersing agents, wetting agents, antifoaming agents, Rheology modifiers, binding agents, solvents, inert carrier, biocides, pH Modifiers, Super-Wetting-spreading-penetrating agent, pH Stabilizers, Stabilizing agents, anti-freezing agent and coloring agents .
Emulsifiers is selected from the group comprising of, but not limited to ethoxylatedpropoxylated alcohols, alkylphenol ethoxylates, alkoxylatedtristyrylphenols, calcium dodecyl benzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylatedricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof; These binders or adhesive imparting agents may be used alone or in combination thereof. The Emulsifiers is present in an amount of from 0.1% to 20.0% by weight based on a total weight of the composition.
Dispersing agent is selected from the group comprising of, but not limited to polymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate/naphthalene sulphonic acid condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate,sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700,butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenolethoxylates and polyalkylene oxide derivative of a synthetic alcohol, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof; The Dispersing agent is present in an amount of from 0.1% to 20.0% by weight based on a total weight of the composition.
Wetting agent is selected from the group comprising of, but not limited to sodium alkyl naphthalene sulfonate, alpha olefin sulfonates, disodium laureth sulfosuccinate, diisodecyl sodium sulfosuccinate, alkyl sulfosuccinic monoesters, dioctyl sulfosuccinate sodium salt, sulfonic acids C14-16-alkane hydroxy and C14-16-alkene sodium salts, polyoxyethylenesorbitanmonooleate, polyoxyethylene ether or mixtures thereof. The Wetting agent is present in an amount of from 0.1% to 15.0% by weight based on a total weight of the composition.
Antifoaming agent is selected from the group comprising of, but not limited to polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof; The Antifoaming agent is present in an amount of from 0.01% to 5.0% by weight based on a total weight of the composition.
Rheology modifier is selected from the group comprising of, but not limited to precipitated silica, fumed silica, modified fumed silica, bentonite, hydroxymethyl cellulose, carboxymethyl cellulose, xanthan gum, thickening silica, hydrated clay minerals, magnesium aluminium silicates, organic derivative of hectorite clay, hydrophobic fumed silica, polyvinylpyrrolidone (PVP) or mixture thereof; The Rheology modifier is present in an amount of from 0.1% to 20.0% by weight based on a total weight of the composition.
Solvents is selected from the group comprising of, but not limited to Demineralized (DM) water, N-alkyl-pyrrolidone, oil medium selected from the group comprising, esterified fatty acids selected from methyl and/or ethyl ester of vegetable oil such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester or mixtures thereof. The Solvents is present in an amount of from 0% to 95% by weight based on a total weight of the composition.
Anti freezing agents is selected from the group comprising of, but not limited to selected from the group comprising of ethylene glycol, propane-1,2-diol, propane-1,2,3-triol, urea or mixtures thereof. The Anti freezing agents is present in an amount of from 0% to 15% by weight based on a total weight of the composition.
Stabilizing agents is selected from the group comprising of, but not limited to selected from the group comprising of epoxidized soyabean oil, butylated hydroxy toluene, ethylenediaminetetraacetic acid, sodium benzoate, etc. or mixtures thereof. The Stabilizing agents is present in an amount of from 0% to 5% by weight based on a total weight of the composition.
Super-Wetting-spreading-penetrating agent is blend of methylated seed oil-organic silicone compound may be selected from methylated seed oil-polyalkyleneoxide modified trisiloxane, methylated seed oil-polyalkyleneoxide modified polydimethylsiloxane, methylated seed oil-20 trisiloxane ethoxylate, Polyalkyleneoxide modified heptamethyltrisiloxane methylated seed oil-polyoxyethylene methyl polysiloxane, methylated seed oil-polyether polymethyl siloxane copolymer, methylated seed oil-polyether modified polysiloxane. The Super-Wetting-spreading-penetrating agent is present in an amount of from 0.1% to 5% by weight based on a total weight of the composition.
PH modifiers is selected from the group comprising of, but not limited to selected from the group comprising of sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, tartaric acid, hydrochloric acid or mixtures thereof. The PH modifier is present in an amount of from 0% to 5% by weight based on a total weight of the composition.
Inert carrier is selected from the group comprising of kaolin, china clay, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, dextrin, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof. The inert carrier is present in an amount of from 0% to 90.0% by weight based on a total weight of the composition.
Binding agent is selected from the group comprising of polyvinylpyrrolidone or mixture thereof or mixtures thereof. The binding agent is present in an amount of from 0.1% to 5.0% by weight based on a total weight of the composition.
Wetting cum dispersing agent is selected from the group comprising of, but not limited to non-ionic proprietary surfactant blend alkylphenol ethoxylates or polyoxyethylene sorbitan esters, lignosulfonates, Mixture of tristyrylphenol ethoxylates andpolyalkylene oxide derivative of a synthetic, alcohol sodium salt of naphthalene sulfonate condensates, tristyrylphenol ethoxylates. In a preferred embodiment, thewetting cum dispersing agentin an amount of from 0.1% to 5.0% by weight based on a total weight of the composition
Biocide is selected from the group comprising of 1,2-benzisothiazolin-3-one, formaldehyde, dipropyl glycol solution of 1,2-benzisothiazolin-3-one or mixtures thereof. The Biocide is present in an amount of from 0.1% to 5.0% by weight based on a total weight of the composition.
pH Stabilizers selected from the group comprising of sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, citric acid, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, hydrochloric acid. The pH Stabilizers is present in an amount of from 0.01% to 10.0% by weight based on a total weight of the composition.
Coloring agent is selected from the group comprising of dye, pigment or mixtures thereof such as Triaryl methane acid blue, 3-hydroxy-N-(2-methylphenyl)-4-[(E)-(2,4,5-trichlorophenyl)diazinyl]-2-naphthamideand Acid Brilliant Green. The Coloring agent is present in an amount of 0.01% to 5.0 % by weight based on a total weight of the composition.
The composition of the present invention is effective for management of insect or pests selected from one or more of Cotton (Gossypium spp.), Paddy (Oryza sativa), Wheat (Triticumaestavum), Barley (Hordeum vulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Sugarcane (Saccharum officinarum) , Sugarbeet (Beta vulgaris), Soybean (Glycin max), Peanut (Arachis hypogaea), Sunflower (Helianthus annuus) , Mustard (Brassica juncea), Rape seed (Brassica napus), Linseed (Linum usitatissimum), Sesame (Sesamum indicum), Castor (Ricinus communis), Green gram (Vigna radiate), Black gram (Vigna mungo), Chickpea (Ciceraritinum), Cowpea (Vigna unguiculata), Redgram (Cajanus cajan), Frenchbean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotyloma uniflorum), Field pea (Pisum sativum), Cluster bean (Cyamopsis tetragonoloba), Lentils (Lens culinaris), Brinjal (Solanum melongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschus esculentus), Onion (Allium cepa L.), Tomato (Solanum lycopersicun) , Potato (Solanum tuberosum) , Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumis sativus), Muskmelons (Cucumis melo), Watermelon (Citrullus lanatus), Bottle gourd (Lagenaria siceraria), Bitter gourd (Momordica charantia), Radish (Raphanus sativus), Carrot (Dacus carota subsp. sativus), Turnip (Brassica rapasubsprapa), Apple (Melus domestica), Banana (Musa 25 spp.), Citrus groups (Citrus spp.), Grape (Vitis vinifera), Guava (Psidium guajava), Litchi (Litchi chinensis), Mango (Mangifera indica), Papaya (Carica papaya), Pineapple (Ananas comosus), Pomegranate (Punica granatum) , Sapota (Manilkara zapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiber officinale), Cumin (Cuminum cyminum), Fenugreek (Trigonella foenum-30 graecum), Fennel (Foeniculum vulgare), Coriander (Coriandrum sativum), Ajwain (Trachyspermumammi), Psyllium (Plantago ovate), Black Pepper (Piper nigrum), Stevia (Stevia rebaudiana), Safedmusli (Chlorophytum tuberosum), Drum stick (Moringa oleifera), Coconut (Coco nucifera), Mentha ( Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold (Tagetes spp.), Common daisy (Bellis perennis), Dahlia (Dahlia hortnesis), Gerbera (Gerbera jamesonii), Carnation (Dianthus caryophyllus) or GMO form thereof.
In yet another preferred embodiment, the present invention provides a insecticidal combination or composition comprising of Cyclaniliprole, Bifenthrin and Diafenthiuron to control the pathogenic microorganism on economically important crops such as rice, chilli,cotton , Brinjal apple, peppers, soybean, cotton, chick pea, Brinjal, pigeon pea, Grapes, Apple and pomegranate, tea, potato, and tomato.
The invention is illustrated by the experiments as exemplified below.
Examples:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 1: Preparation of insecticidal composition as Suspension concentrates (SC):
In an embodiment, the chemical composition of the present insecticidal is depicted below in Table 1 (a) below:

Table 1(a): Insecticidal Composition for Suspension concentrates (SC).
Component Composition (%w/w) Function
Cyclaniliprole 4.5 Active Ingredient
Bifenthrin 5.5 Active Ingredient
Diafenthiuron 38.0 Active Ingredient
Nonionic proprietary surfactant blend 2.00 Dispersing agent
Acrylic copolymer 2.00 Dispersing agent
Sodium salt of naphthalene sulfonate condensate 1.50 Dispersing agent
Polydimethylsiloxane emulsion 0.70 Antifoaming agent
Propane-1,2,3-triol 6.50 Ant freezing Agent
Dipropylene glycol solution of 1,2-benzisothiazolin-3-one 0.10 Biocide
Precipitated Silica 1.00 Inert carrier
Citric acid 0.30 pH stabilizer
Sodium Benzoate 1.00 Stabilizing agent
Xanthum gum (2 % w/w aqueous of Rhodopol 23) 5.00 Rheology modifier
Demineralised water Balance to 100 Solvent

Process for preparing Suspension concentrates (SC)formulation:
In demineralized water other constituents namely dipropylene glycol solution of 1,2-benzisothiazolin-3-one, polydimethylsiloxane emulsion, propane-1,2,3-triol, nonionic proprietary surfactant blend, acrylic copolymer and sodium salt of naphthalene sulfonate condensate as mentioned in Table 1 were added into the clean pre-mixing vessel fitted with the homogenizer and all the ingredients were mixed for sufficient time with the homogenizer to obtain a mixture. The active ingredients, Cyclaniliprole, Bifenthrin and Diafenthiuron as well as precipitated silica, pH modifier and stabilizer were added to the obtained mixture and continued to mix for sufficient time with the homogenizer to obtain a slurry. The slurry was passed through jacketed bead mill with chilled water circulation for particle size reduction to obtain a milled slurry of desirable particle size of d(90)<20 micron. The milled slurry was collected into post mixing vessel fitted with the stirrer. Rheology modifier as 2 % Xanthum gum (of Rhodopol 23) in demineralized water was added into the post mixing vessel containing grinded slurry and continued to mix for sufficient time up to proper mixing of Xanthum gum to form homogeneousinsecticidal suspension concentrate (SC) composition.
Example 2: Preparation of insecticidal composition as Flowable Suspension for Seed Treatment (FS) Formulation:
Table-1 (b) :Insecticidal Composition of the Flowable Suspension for Seed Treatment (FS) Formulation.
Component Composition (%w/w) Remark
Cyclaniliprole 4.5 Active Ingredient
Bifenthrin 5.5 Active Ingredient
Diafenthiuron 38.0 Active Ingredient
Nonionic proprietary surfactant blend
2.00 Wetting cum dispersing agent
Acrylic copolymer 3.00 Dispersing agent
Sodium salt of naphthalene sulfonate condensate 1.50 Dispersing agent
Propane-1,2,3-triol 7.00 Antifreezing Agent
Dipropylene glycol solution of 1,2-benzisothiazolin-3-one 0.10 Biocide
Polydimethylsiloxane emulsion 0.70 Antifoaming agent
Precipitated Silica 1.00 Stabilising agent
Triaryl methane acid blue 0.10 Coloring agent
Citric acid 0.50 pH modifier
Sodium Benzoate 1.00 Stabilizing agent
Xanthum gum (2 % w/w aqueous of Rhodopol 23) 5.00 Rheology modifier
Demineralized Water Balance to 100 Solvent

Process for preparing Flowable suspension for seed treatment (FS) formulation:
In demineralized water along with other constituents namely dipropylene glycol solution of 1,2-benzisothiazolin-3-one, polydimethylsiloxane emulsion, propane-1,2,3-triol, nonionic proprietary surfactant blend, acrylic copolymer and sodium salt of naphthalene sulfonate condensate were added into the clean pre-mixing vessel fitted with the homogenizer and all the ingredients were mixed for sufficient time with the homogenizer to obtain a mixture. The active ingredients, Cyclaniliprole, Bifenthrin and Diafenthiuron as well as precipitated silica, pH modifier, stabilizer and coloring agentwere added to the obtained mixture and continued to mix for sufficient time with the homogenizer to obtain a slurry. The slurry was passed through jacketed bead mill with chilled water circulation for particle size reduction to obtain a milled slurry of desirable particle size of d(90)<20 micron. The milled slurry was collected into post mixing vessel fitted with the stirrer. Rheology modifier as 2 % Xanthum gum (of Rhodopol 23) in demineralized water was added into the post mixing vessel and continued to mix for sufficient time up to proper mixing of Xanthum gum to form Flowable suspension for seed treatment (FS) composition of insecticide.
Example 3: Preparation of insecticidal composition as Oil Dispersion (OD) formulation:
Table-1 (c) :Insecticidal Composition of the Oil Dispersion (OD) formulation
Component Composition (%w/w) Remark
Cyclaniliprole 4.5 Active Ingredient
Bifenthrin 5.5 Active Ingredient
Diafenthiuron 38.0 Active Ingredient
Castor oil ethoxylate (10 mole) 6.00 Emulsifier
Calcium dodecylbenzenesulfonate 2.00 Emulsifier
Mixture of fatty acid polyethylene glycol ester 4.00 Emulsifier
Polymeric ester dispersant 0.5 Dispersing agent
Ethoxylated polyarylphenol phosphate ester 0.5 Dispersing agent
Propane-1,2,3-triol 2.00 Antifreezing Agent
Polyalkyleneoxide modified heptamethyltrisiloxane 1.00 Super-Wetting-spreading-penetrating agent
Polydimethylsiloxane 0.30 Antifoaming agent
Fumed silica 1.50 Rheology modifier
Citric acid 0.10 pH stabilizer
Sodium Benzoate 1.00 Stabilizing agent
Bentonite clay 1.50 Rheology modifier
Methyl ester of vegetable oil Balance to 100 Solvent

Process for preparing Oil Dispersion (OD) formulation:
In methyl ester of vegetable oilconstituents namely, polyalkyleneoxide modified heptamethyltrisiloxane, polydimethylsiloxane, propane-1,2,3-triol, castor oil ethoxylate(10 mole),calcium dodecylbenzenesulfonate, ethoxylated polyarylphenol phosphate ester, mixture of fatty acid polyethylene glycol ester and polymeric ester dispersant were added into the clean pre-mixing vessel fitted with the homogenizer and all the ingredients were mixed for sufficient time with the homogenizer to obtain a mixture. The active ingredients, Cyclaniliprole, Bifenthrin and Diafenthiuron as well as pH stabilizer and stabilizing agent were added to the obtained mixture and continued to mix for sufficient time with the homogenizer to obtain a slurry. The slurry was passed through jacketed bead mill with chilled water circulation for particle size reduction to obtain a milled slurry of desirable particle size of d(90)<20 micron. The milled slurry was collected into post mixing vessel fitted with the stirrer. To milled slurry bentonite clayand fumed silica were added under stirring into the post mixing vessel and continued to mix for sufficient time up to proper mixing to form Insecticidal Oil Dispersion (OD) formulation
Example 4: Preparation of insecticidal composition as Water dispersible granules (WDG) formulation:
Table-1 (d): Insecticidal Composition of Water dispersible granules (WDG)formulation.
Component Composition (%w/w) Remark
Cyclaniliprole 4.5 Active Ingredient
Bifenthrin 5.5 Active Ingredient
Diafenthiuron 38.0 Active Ingredient
Sulfonic acids, C14-16-alkane hydroxy and C14-16-alkene, sodium salts 5.00 Wetting agent
Dispertox BS SPL 7.00 Dispersing agent
Sodium ligno sulfonate 5.00 Dispersing agent
Tersperse 2700 5.00 Dispersing agent
Aryl sulphonate condensate 5.00 Dispersing agent
Attagel50 1.00 Inert carrier
Precipitated silica 2.00 Inert carrier
Polydimethylsiloxane emulsion 1.00 Antifoaming agent
Polyalkyleneoxide modified heptamethyltrisiloxane 1.00 Super-Wetting-spreading-penetrating agent
Kaolin Balance to 100 Inert carrier

Process for preparing Water dispersible granules (WDG) formulation
Pre-blending of Cyclaniliprole, Bifenthrin, Diafenthiuron and other agrochmemical acceptable auxiliaries to obtain a homogenous mixture. Milling the blended mixture to obtain particle size in the range of below 40micron with suitable equipment. Post blending the milled material and preparation of dough with water. Extrusion of dough with suitable extruder. Drying of granules and sieving through suitable size sieves to obtain Insecticidal Water Dispersible Granules.
Example 5: Preparation of insecticidal composition as Wettable powder (WP)) formulation:
Table-1 (d): Insecticidal Composition of Wettable powder (WP) formulation.
Component Composition (%w/w) Remark
Cyclaniliprole 4.5 Active Ingredient
Bifenthrin 5.5 Active Ingredient
Diafenthiuron 38.0 Active Ingredient
Sulfonic acids, C14-16-alkane hydroxy and C14-16-alkene, sodium salts 5.00 Wetting agent
Dispertox BS SPL 4.00 Dispersing agent
Sodium ligno sulfonate 3.00 Dispersing agent
Terwet 2700 2.00 Dispersing agent
Aryl sulphonate condensate 3.00 Dispersing agent
Attagel 50 1.50 Inert carrier
Precipited silica 5.00 Inert carrier
Polydimethylsiloxane emulsion 1.00 Antifoaming agent
Polyalkyleneoxide modified heptamethyltrisiloxane 1.00 Super-Wetting-spreading-penetrating agent
China clay Balance to 100 Inert carrier

Process for preparing wettable powder (WP)formulation:
Pre-blending Cyclaniliprole, Bifenthrin, Diafenthiuron and other agrochmemical acceptable auxiliaries to obtain a homogenous mixture. Milling the blended mixture to obtain particle size in the range of below 50 micron with suitable equipment. Post blending the milled material to obtain Insecticidal wettablePowdercomposition

Stability Data
Stability Study (Accelerated Storage test and low-temperature storage test)
According to the FAO/WHO manual, the “accelerated storage test” is considered as an indicative of product stability. That is, accelerated storage test data provides an indication that the product is stable for at least two years at ambient temperature. Further, the FAO/WHO manual indicates storage at 54 ± 2°C for 14 days as the default test conditions. Further, the FAO/WHO manual indicates low-temperature storage at 0 ± 2°C for 7 days.
The “accelerated storage” is the sample after subjecting the sample to accelerated storage tests at 54±2°C for 14 days.
The insecticidal formulation composition of examples 1 - 3 were found stable after accelerated stability study at 54 ± 2° C for 14 days and low temperature at 0 ± 2°C for 7 days. The insecticidal formulation composition of examples 5 - 6 were found stable after accelerated stability study at 54 ± 2° C for 14 days.
Synergistic effect of the different Insecticidal Composition:
Colby’s method for ternary mixes was used for checking synergistic effects. E (expected efficacy) in the Colby’s method can be expressed as for a specific combination of three active components’:
E=A+B+C-((AB+AC+BC))/100+((ABC))/1000
Where, E = expected efficacy,
A, B and C = the efficacy of three active ingredients A, B and C at a given dose.
Synergy ratio (R)=(Experimentally observed efficacy (O))/(Expected efficacy (E))
If the synergism ratio (R) between observed and expected is > 1 then synergy is exhibited, if R= 1 then the effect is additive and if R< 1 then the mix is antagonistic.
The experimental data was statistically analyzed by Randomized Block Design (RBD) (One factor analysis) using OPSTAT HAU statistical software. The results are expressed as Mean ± SE (standard error) and data was statistically analyzed by one- way Analysis of variance (ANOVA), with the level of significance set at p < 0.01.
Evaluation of Bio-efficacy on Brinjal
In Asia, eggplant or brinjal (Solanummelongena) is one of the most widely growing and significant crops in terms of the economy. One of the very few vegetables that are affordable for the poor in both rural and urban areas, eggplant is grown all year round, especially during the hot and humid monsoon season when other vegetables are lacking. It also provides farmers with a crucial source of financial income. In addition to providing money, eggplant is a valuable source of nutrients, including dietary fiber, folic acid, minerals including magnesium, phosphorus, potassium, manganese, and copper, and vitamins B6, C, K, thiamin, niacin, and pantothenic acid. However, the cultivation of eggplant has somewhat decreased recently due to the rise in insect pest populations, especially Fruit and Shoot borer, Whitefly, Thrips, Jassids and some other pests.

Table 2 :Effect of treatment Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC on population of Fruit borer (Brinjal)-
Sr. No.
Treatment Compositions Dosage/ha % Fruit infestation
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % reduction over control 10 DAT Colby value 10 DAT 15 DAT % reduction over control 15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 8.47
(16.89) 9.52
(17.83) 56.15 10.45
(18.96) 55.91
2 Bifenthrin 10% EC 50 500 9.62
(18.04) 10.36
(18.71) 52.28 11.72
(19.66) 50.55
3 Diafenthiuron 50% WP 300 600 10.58
(18.96) 11.23
(19.10) 48.27 13.34
(22.18) 43.71
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.19
(10.26) 2.21
(9.28) 89.82 1.01 2.63
(9.71) 88.90 1.01
5 Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC 17.50 + 22.50 + 170 500 4.86
(12.68) 3.76
(10.85) 82.68 0.93 4.81
(12.74) 79.70 0.91
6 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 21 + 27 + 204 600 4.14
(11.72) 3.29
(10.41) 84.85 0.95 3.87
(11.19) 83.67 0.95
7 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 24.50 + 31.50 + 238 700 3.35
(10.46) 2.75
(9.82) 87.33 0.98 3.72
(10.81) 84.14 0.96
8 Untreated Check - - 17.84
(24.97) 21.71
(28.05) - 23.70
(29.12) -
CD at 5% (2.19) (2.61) (1.74)
SE (m) (0.68) (0.85) (0.57)
DAT: Days After Treatment; Figures in parenthesis are arc sin transformed values

Among all the doses rates of combination and individualtreatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC @ 24.50 + 31.50 + 238 gm a.i./ha and @ 21 + 27 + 204 gm a.i./ha, treated plots were observed with highest per cent reduction over control, along with lowest per cent fruit infestation by fruit borer at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed 88.90%, 84.14% and 83.67% reduction over control at 15 DAT, respectively.
All the doses of Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (55.91%), Bifenthrin 10% EC @ 50 gm a.i./ha (50.55%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (43.71%) against Fruit borer of brinjal at 15 DAT. The application of Cyclaniliprole 10% DC + Bifenthrin 10% EC + Diafenthiuron 50% WP @ 40+50+300 gm a.i./ha showed synergistic effect with the synergistic ratio 1.01 (>1) at 15 DAT (Table 2)

Table 3: Table 3: Effect of different Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC treatments on population of Fruit borer (Brinjal)-
Sr. No.
Treatment Compositions Dosage/ha % Fruit infestation
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % reduction over control 10 DAT Colby value 10 DAT 15 DAT % reduction over control 15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 9.32
(17.64) 9.58
(17.79) 52.95 10.21
(18.44) 53.86
2 Bifenthrin 10% EC 50 500 10.25
(18.47) 10.13
(18.36) 50.25 11.43
(19.28) 48.35
3 Diafenthiuron 50% WP 300 600 11.36
(19.23) 11.64
(19.72) 42.83 13.34
(21.17) 39.71
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.38
(10.51) 2.14
(9.16) 89.49 1.03 2.25
(9.20) 89.83 1.05
5 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 22.50 + 27.50 + 190 500 5.13
(13.07) 3.57
(10.73) 82.47 0.95 4.30
(11.15) 80.57 0.94
6 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 27 + 33 + 228 600 2.45
(9.56) 1.80
(6.13) 91.16 1.05 2.17
(9.19) 90.19 1.05
7 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 31.50 + 38.50 + 266 700 1.98
(6.15) 1.72
(6.05) 91.55 1.06 2.11
(9.08) 90.47 1.06
8 Untreated Check - - 18.54
(25.63) 20.36
(26.72) - 22.13
(28.54) -
CD at 5% (2.35) (2.51) (1.83)
SE (m) (0.59) (0.84) (0.47)
DAT: Days After Treatment; Figures in parenthesis are arc sin transformed values

Results:
Among all the doses rates of combination and individualtreatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, treated plots were observed with highest per cent reduction over control, along with lowest per cent fruit infestation by fruit borer at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed at par results with 89.83%, 90.47% and 90.19% reduction over control at 15 DAT, respectively.
All the doses of Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (53.86%), Bifenthrin 10% EC @ 50 gm a.i./ha (48.35%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (39.71%) against Fruit borer of brinjal at 15 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.05, 1.06 & 1.05 respectively (>1) at 15 DAT (Table 3).

Table 4: Effect of Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC treatments on Fruit borer (Brinjal)-
Sr. No.
Treatment Compositions Dosage/ha % Fruit infestation
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % reduction over control 10 DAT Colby value 10 DAT 15 DAT % reduction over control 15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 8.32
(16.75) 7.45
(15.24) 59.66 9.65
(18.12) 55.01
2 Bifenthrin 10% EC 50 500 9.74
(18.16) 10.38
(18.71) 43.80 11.27
(19.16) 47.46
3 Diafenthiuron 50% WP 300 600 10.55
(18.93) 11.19
(19.08) 39.42 12.86
(20.54) 40.05
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 1.97
(7.24) 1.83
(7.14) 90.09 1.04 2.28
(9.31) 89.37 1.04
5 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 27.50 + 32.50 + 205 500 4.62
(11.87) 2.79
(9.76) 84.89 0.98 3.52
(10.47) 83.59 0.97
6 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 33 + 39 + 246 600 2.58
(9.62) 1.64
(6.07) 91.12 1.06 1.98
(7.23) 90.82 1.06
7 Cyclaniliprole 5.5% +Bifenthrin6.5% +Diafenthiuron 41% SC 38.50 + 45.50 + 287 700 1.85
(7.17) 1.20
(5.86) 93.50 1.08 1.71
(7.09) 92.03 1.07
8 Untreated Check - - 16.21
(23.21) 18.47
(25.54) - 21.45
(27.86) - -
CD at 5% (2.24) (2.65) (1.75)
SE (m) (0.64) (0.72) (0.58)
DAT: Days after Treatment; Figures in parenthesis are arc sin transformed values

Results:
Among all the doses rates of combination and individualtreatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, treated plots were observed with highest per cent reduction over control, along with lowest per cent fruit infestation by fruit borer at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed at par results with 89.37%, 92.03% and 90.82% reduction over control at 15 DAT, respectively.
All the doses of Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (55.01%), Bifenthrin 10% EC @ 50 gm a.i./ha (47.46%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (40.05%) against Fruit borer of brinjal at 15 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.04, 1.07 & 1.06 respectively (>1) at 15 DAT (Table 4).
Phytotoxicity observations
For the evaluation of phytotoxicity on brinjal, cotton and tomato crops, observations were made by observing the temporary or long lasting damage to the leaves if any viz., yellowing, wilting, necrosis, epinasty and hyponasty at 5, 10 and 15DAT of the synergistic insecticidal composition of the present invention. Crop injury was observed on visual rating from 0-10 scale.

Phytotoxicity rating scale
Rating Crop Injury (%) Verbal Description
0 - No symptoms
1 1-10 Verbal slight discoloration
2 11-20 More severe, but not lasting
3 21-30 Moderate and more lasting
4 31-40 Medium and lasting
5 41-50 Moderately heavy
6 51-60 Heavy
7 61-70 Very heavy
8 71-80 Nearly destroyed
9 81-90 Destroyed
10 91-100 Completely destroyed

Table 5: Phytotoxic effect of synergistic composition of the present invention on Brinjal.
Treatments Phytotoxicity rating
Yellowing Wilting Necrosis Epinasty Hyponasty
Days After Spray
5 10 15 5 10 15 5 10 15 5 10 15 5 10 15
T1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Conclusion
After evaluation of three formulations i.e., Cyantraniliprole 7.5% + Bifenthrin 4.0% + Cartap hydrochloride 27.5%, Cyantraniliprole 8.5% + Bifenthrin 4.5% + Cartap hydrochloride 35.5% and Cyantraniliprole 9.5% + Bifenthrin 6.0% + Cartap hydrochloride 40% at three different doses (600 ml/ ha, 700 ml/ha & 800 ml/ha). Cyantraniliprole 8.5% + Bifenthrin 4.5% + Cartap hydrochloride 35.5% @ 700 ml/ ha found to be effective against Leaf folder of Rice crop in comparison to Tank mix formulation (Cyantraniliprole 10.26% OD + Bifenthrin 10% EC + Cartap hydrochloride 50% SP) as well as solo treatments (Cyantraniliprole 10.26% OD, Bifenthrin 10% EC & Cartap hydrochloride 50% SP). Cyantraniliprole 8.5% + Bifenthrin 4.5% + Cartap hydrochloride 35.5% @ 700 ml/ ha was found at par with higher formulation, higher dose and higher active ingredient.
Therefore, application of Cyantraniliprole 8.5% + Bifenthrin 4.5% + Cartap hydrochloride 35.5% @ 700 ml/ ha (59.50 + 31.50 + 248.50 gm a.i/ ha) formulation can be recommended for effective control of Leaf folder in Rice crop.
Evaluation of Bio efficacy on Cotton
Cotton (Gossypium spp.) is one of most significant natural fiber crop, also known as "white gold," which is essential to trade, the economy, industry, employment, and foreign exchange profits. It is the main cash crop for the nation and is referred to as the "king of fiber". India has the largest cotton acreage in the world12.19 million hectares and produces the second-highest number of bales 37.10 million after China. Cotton production suffers greatly from its vulnerability to around 162 different kinds of insect pests. Bollworms, Jassids, Thrips, Aphids, and Whitefly pests, are the most common pests of cotton crop.

Table 6: Effect of Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC treatments on population of Bollworm (Cotton)-
Sno
Treatment Compositions Dosage/ha Average no. of Boll worm larvae per 10 plants
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT 15 DAT % Mortality15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 9.53
(3.19) 10.37
(3.36) 53.62 11.45
(3.69) 52.69
2 Bifenthrin 10% EC 50 500 10.83
(3.55) 11.42
(3.67) 48.93 12.18
(3.76) 49.54
3 Diafenthiuron 50% WP 300 600 12.47
(3.72) 13.45
(3.85) 39.85 15.53
(4.43) 35.67
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.98
(2.53) 3.26
(2.01) 85.42 1.00 3.36
(2.05) 86.08 1.02
5 Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC 17.50 + 22.50 + 170 500 5.35
(2.81) 4.63
(2.37) 79.29 0.92 5.21
(2.76) 78.42 0.93
6 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 21 + 27 + 204 600 5.14
(2.75) 4.76
(2.43) 78.71 0.92 4.17
(2.12) 82.73 0.98
7 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 24.50 + 31.50 + 238 700 4.25
(2.20) 3.51
(2.15) 84.30 0.98 4.49
(2.35) 81.40 0.96
8 Untreated Check - - 18.58
(4.63) 22.36
(4.92) - 24.14
(5.08) -
CD at 5% (0.19) (0.21) (0.23)
SE (m) (0.03) (0.04) (0.06)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individual ltreatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC @ 24.50 + 31.50 + 238 gm a.i./ha and @ 21 + 27 + 204 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Bollworms larvae per 10 plants at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed results with 86.08%, 81.40% and 82.73% mortality at 15 DAT, respectively.
All the doses of Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (52.69%), Bifenthrin 10% EC @ 50 gm a.i./ha (49.54%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (35.67%) against Bollworms larvae of cotton at 15 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10% EC + Diafenthiuron 50% WP @ 40+50+300 gm a.i./ha showed synergistic effect with the synergistic ratio 1.02 (>1) at 15 DAT (Table 6).

Table 7: Effect of Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC treatments on population of Bollworm (Cotton)-
Sr. No.
Treatment Compositions Dosage/ha Average no. of Boll worm larvae per 10 plants
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT 15 DAT % Mortality15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 10.24
(3.23) 9.58
(3.16) 54.42 11.15
(3.35) 51.82
2 Bifenthrin 10% EC 50 500 11.56
(3.42) 10.61
(3.54) 49.52 13.64
(3.81) 41.05
3 Diafenthiuron 50% WP 300 600 13.65
(3.85) 12.47
(3.67) 40.68 14.56
(4.06) 37.08
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.87
(2.77) 2.32
(1.64) 88.96 1.03 2.78
(1.77) 87.99 1.07
5 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 22.50 + 27.50 + 190 500 5.76
(2.63) 4.16
(2.38) 80.21 0.93 5.17
(2.25) 77.66 0.95
6 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 27 + 33 + 228 600 2.69
(1.75) 2.27
(1.66) 89.20 1.03 2.89
(1.80) 87.51 1.07
7 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 31.50 + 38.50 + 266 700 2.17
(1.57) 2.03
(1.12) 90.34 1.05 2.54
(1.47) 89.02 1.08
8 Untreated Check - - 19.10
(4.80) 21.02
(4.75) - 23.14
(5.01) -
CD at 5% (0.23) (0.25) (0.33)
SE (m) (0.04) (0.05) (0.07)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individual treatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Bollworms larvae per 10 plants at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed at par results with 87.99%, 89.02% and 87.51% mortality at 15 DAT, respectively.
All the doses of Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (51.82%), Bifenthrin 10% EC @ 50 gm a.i./ha (41.05%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (37.08%) against Bollworms larvae of cotton at 15 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.07, 1.08 & 1.07 respectively (>1) at 15 DAT (Table 7).

Table 8: Effect of Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC treatments on population of Bollworm (Cotton)-
Sr. No.
Treatment Compositions Dosage/ha Average no. of Boll worm larvae per 10 plants
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT 15 DAT % Mortality15 DAT Colby value 15 DAT
1 Cyclaniliprole 10% DC 40 400 8.65
(3.27) 8.16
(3.14) 57.32 10.15
(3.40) 53.10
2 Bifenthrin 10% EC 50 500 10.14
(3.39) 10.95
(3.66) 42.73 12.67
(3.84) 41.45
3 Diafenthiuron 50% WP 300 600 11.46
(3.75) 13.53
(3.92) 29.24 14.21
(4.03) 34.33
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 2.19
(1.52) 2.31
(1.63) 87.92 1.06 2.84
(1.76) 86.88 1.06
5 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 27.50 + 32.50 + 205 500 5.37
(2.78) 4.25
(2.19) 77.77 0.94 4.18
(2.13) 80.68 0.98
6 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 33 + 39 + 246 600 3.11
(2.04) 2.14
(1.58) 88.81 1.07 2.28
(1.67) 89.46 1.09
7 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 38.50 + 45.50 + 287 700 2.06
(1.17) 1.59
(1.36) 91.68 1.11 1.82
(1.49) 91.59 1.12
8 Untreated Check - - 17.33
(4.63) 19.12
(4.75) - 21.64
(4.88) -
CD at 5% (0.24) (0.26) (0.28)
SE (m) (0.05) (0.07) (0.09)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individual treatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Bollworms larvae per 10 plants at 5th, 10th and 15th days after treatment (DAT) in comparison with other treatments. All the three doses showed results with 86.88%, 91.59% and 89.46% mortality at 15 DAT, respectively.
All the doses of Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (53.10%), Bifenthrin 10% EC @ 50 gm a.i./ha (41.45%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (34.33%) against Bollworms larvae of cotton at 15 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.06, 1.12 & 1.09 respectively (>1) at 15 DAT (Table 8).

Table 9: Effect of Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC treatments on population of Jassid (Cotton)-
Sr.No. Treatment compositions Dose/ha Average no. Jassid per leaf
Dosage/ ha a.i. (gm) Formulation (gm/ml) 3 DAT 5 DAT % Mortality 5 DAT Colby value 5 DAT 7 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT
1 Cyclaniliprole 10% DC 40 400 15.42
(4.27) 16.25
(4.68) 35.34 15.36
(4.84) 19.16
(5.39) 34.70
2 Bifenthrin 10% EC 50 500 13.25
(3.83) 14.15
(4.06) 43.69 13.21
(3.81) 16.37
(4.70) 44.21
3 Diafenthiuron 50% WP 300 600 9.13
(3.15) 9.47
(3.24) 62.32 8.72
(3.04) 11.52
(3.79) 60.74
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.83
(2.68) 2.58
(1.72) 89.73 1.04 2.67
(1.78) 3.75
(2.61) 87.22 1.02
5 Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC 17.50 + 22.50 + 170 500 5.79
(2.74) 5.33
(2.52) 78.79 0.91 4.25
(2.16) 4.83
(2.73) 83.54 0.97
6 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 21 + 27 + 204 600 4.21
(2.15) 4.28
(2.19) 82.97 0.96 3.18
(2.25) 4.52
(2.47) 84.59 0.99
7 Cyclaniliprole 3.5 % +Bifenthrin 4.5% +Diafenthiuron 34% SC 24.50 + 31.50 + 238 700 3.42
(2.37) 3.84
(2.69) 84.72 0.98 3.58
(2.42) 4.48
(2.39) 84.73 0.99
8 Untreated Check - - 21.52
(4.92) 25.13
(5.08) - 27.31
(5.36) 29.34
(5.87) -
CD at 5% (0.27) (0.29) (0.30) (0.33)
SE (m) (0.06) (0.08) (0.09) (0.10)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individualtreatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC @ 24.50 + 31.50 + 238 gm a.i./ha and @ 21 + 27 + 204 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Jassid per leaf at 3rd, 5th, 7th and 10th days after treatment (DAT) in comparison with other treatments. All the three doses showed results with 87.22%, 84.73% and 84.59% mortality at 10 DAT, respectively.
All the doses of Cyclaniliprole 3.5% + Bifenthrin 4.5% + Diafenthiuron 34% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (34.70%), Bifenthrin 10% EC @ 50 gm a.i./ha (44.21%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (60.74%) against Jassid of Cotton at 10 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10% EC + Diafenthiuron 50% WP @ 40+50+300 gm a.i./ha showed synergistic effect with the synergistic ratio 1.02 (>1) at 10 DAT (Table 9).

Table 10: Effect of Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC treatments on population of Jassid (Cotton)-
Sr.No. Treatment compositions Dose/ha Average no. Jassid per leaf
Dosage/ ha a.i. (gm) Formulation (gm/ml) 3 DAT 5 DAT % Mortality 5 DAT Colby value 5 DAT 7 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT
1 Cyclaniliprole 10% DC 40 400 16.36
(4.15) 19.36
(6.27) 26.47 18.15
(6.14) 19.35
(6.23) 36.25
2 Bifenthrin 10% EC 50 500 14.21
(4.08) 16.23
(4.12) 38.36 15.28
(4.10) 17.13
(5.42) 43.60
3 Diafenthiuron 50% WP 300 600 10.73
(3.19) 12.65
(3.87) 51.96 11.46
(3.71) 12.42
(3.84) 59.10
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 3.27
(2.08) 2.42
(1.76) 90.81 1.16 2.14
(1.78) 2.56
(1.80) 91.57 1.07
5 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 22.50 + 27.50 + 190 500 6.53
(2.87) 6.26
(2.72) 76.22 0.97 5.13
(2.26) 5.11
(2.22) 83.17 0.98
6 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 27 + 33 + 228 600 4.12
(2.15) 2.87
(1.82) 89.10 1.14 2.75
(1.81) 3.26
(3.07) 89.27 1.05
7 Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC 31.50 + 38.50 + 266 700 3.59
(2.13) 2.18
(1.79) 91.72 1.17 2.10
(1.73) 2.98
(1.85) 90.19 1.06
8 Untreated Check - - 22.65
(5.07) 26.33
(5.64) - 29.26
(5.73) 30.37
(5.92) -
CD at 5% (0.25) (0.29) (0.31) (1.23)
SE (m) (0.08) (0.10) (0.13) (0.20)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individual treatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Jassid per leaf at 3rd, 5th, 7th and 10th days after treatment (DAT) in comparison with other treatments. All the three doses showed at par results with 91.57%, 90.19% and 89.27% mortality at 10 DAT, respectively.
All the doses of Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (36.25%), Bifenthrin 10% EC @ 50 gm a.i./ha (43.60%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (59.10%) against Jassid of Cotton at 10 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 4.5% + Bifenthrin 5.5% + Diafenthiuron 38% SC @ 31.50 + 38.50 + 266 gm a.i./ha and @ 27 + 33 + 228 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.07, 1.06 & 1.05 respectively (>1) at 10 DAT (Table 11).

Table 12: Effect of Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC treatments on population of Jassid (Cotton)-
Sr.No. Treatment compositions Dose/ha Average no. Jassid per leaf
Dosage/ ha a.i. (gm) Formulation (gm/ml) 3 DAT 5 DAT % Mortality 5 DAT Colby value 5 DAT 7 DAT 10 DAT % Mortality 10 DAT Colby value 10 DAT
1 Cyclaniliprole 10% DC 40 400 14.38
(3.95) 15.67
(4.58) 38.38 17.35
(5.07) 19.13
(6.13) 34.58
2 Bifenthrin 10% EC 50 500 11.27
(3.24) 13.27
(3.86) 47.82 14.21
(3.76) 16.35
(4.82) 44.09
3 Diafenthiuron 50% WP 300 600 8.46
(2.85) 9.13
(3.07) 64.10 7.43
(2.72) 10.24
(3.16) 64.98
4 Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP 40 +50+300 400+500+600 4.62
(2.46) 2.65
(1.89) 89.58 1.01 2.20
(1.53) 3.19
(2.08) 89.09 1.02
5 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 27.50 + 32.50 + 205 500 4.76
(2.54) 4.23
(2.18) 83.37 0.94 3.86
(2.10) 4.14
(2.11) 85.84 0.98
6 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 33 + 39 + 246 600 4.13
(2.10) 2.10
(1.36) 91.74 1.04 2.16
(1.48) 2.53
(1.76) 91.35 1.05
7 Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC 38.50 + 45.50 + 287 700 2.42
(1.67) 1.78
(1.31) 93.00 1.05 1.62
(1.24) 2.17
(1.50) 92.58 1.06
8 Untreated Check - - 21.35
(4.93) 25.43
(5.16) - 27.61
(5.73) 29.24
(5.89) -
CD at 5% (0.26) (0.28) (0.30) (0.32)
SE (m) (0.05) (0.09) (0.12) (0.14)
DAT: Days After Treatment; Figures in parenthesis are square root transformed values

Among all the doses rates of combination and individual treatment, Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, treated plots were observed with highest per cent mortality, along with lowest no. of population of Jassid per leaf at 3rd, 5th, 7th and 10th days after treatment (DAT) in comparison with other treatments. All the three doses showed at par results with 89.09%, 92.58% and 91.35% mortality at 10 DAT, respectively.
All the doses of Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC showed better result compared to individual application of Cyclaniliprole 10% DC @ 40 gm a.i./ha (34.58%), Bifenthrin 10% EC @ 50 gm a.i./ha (44.09%) and Diafenthiuron 50% WP @ 300 gm a.i./ha (64.98%) against Jassid of Cotton at 10 DAT.
The application of Cyclaniliprole 10% DC + Bifenthrin 10 % EC + Diafenthiuron 50% WP @ 40 + 50 + 300 gm a.i./ha and Cyclaniliprole 5.5% + Bifenthrin 6.5% + Diafenthiuron 41% SC @ 38.50 + 45.50 + 287 gm a.i./ha and @ 33 + 39 + 246 gm a.i./ha, showed synergistic effect with the synergistic ratio 1.02, 1.06 & 1.05 respectively (>1) at 10 DAT (Table 12).
Table 13: Phytotoxic effect of synergistic composition of the present invention on Cotton
Treatments Phytotoxicity rating
Yellowing Wilting Necrosis Epinasty Hyponasty
Days After Spray
5 10 15 5 10 15 5 10 15 5 10 15 5 10 15
T4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Conclusion
After evaluation of three formulations i.e., Cyclaniliprole 3.5% +Bifenthrin 4.5% + Diafenthiuron 34% SC, Cyclaniliprole 4 .5% +Bifenthrin 5.5% +Diafenthiuron 38% SC and Cyclaniliprole 5.5% +Bifenthrin 6.5% +Diafenthiuron 41% SC at three different doses (500 ml/ ha, 600 ml/ha & 700 ml/ha). Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC @ 600 ml/ ha found to be effective against Fruit borer and Whitefly of Brinjal crop, along with Bollworm and Jassid of Cotton crop in comparison to Tank mix formulation (Cyclaniliprole 10% DC +Bifenthrin 10% EC + Diafenthiuron 50% WP) as well as solo treatments (Cyclaniliprole 10% DC, Bifenthrin 10% EC &Diafenthiuron 50% WP). Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC @ 600 ml/ ha was found at par with higher formulation, higher dose and higher active ingredient.
Therefore, application of Cyclaniliprole 4.5% +Bifenthrin 5.5% +Diafenthiuron 38% SC @ 600 ml/ ha (27 + 33 + 228 gm a.i/ ha) formulation can be recommended for effective control of Fruit borer & Whitefly of Brinjal crop and Bollworm &Jassid of Cotton crop. , Claims:
1. An insecticidal composition, comprising:
(a) 0.1% to about 25.0% Cyclaniliprole, by weight of the composition;
(b) 0.1% to about 25.0% Bifenthrin by weight of the composition ;
(c) 0.1% to about 50.0% Diafenthiuron by weight of the composition; and
(d) agriculturally accepted excipient.

2. The insecticidal composition as claimed in claim 1, wherein the agriculturally accepted excipient is selected from the group comprising emulsifier; dispersing agent; wetting agent; antifoaming agent; Wetting cum dispersing agent; rheology modifier; solvent; pH modifier; inert carrier; pH stabilizers; Super-Wetting-spreading-penetrating agent; biocide; binding agent; an antifreezing agent; stabilizing agent; and coloring agent wherein the composition comprises- about 0.1% to about 20.0% emulsifier; about 0.1% to about 20.0% dispersing agent; about 0.1% to about 20.0% biocide; about 0.1% to about 15.0% wetting agent; about 0.01% to about 5.0% antifoaming agent; about 0.1% to 5.0% Wetting cum dispersing agent ; about 0.01% to about 20.0% rheology modifier; about 0% to about 95.0% solvent; about 0% to about 90.0 % inert carrier; about 0% to about 15 % antifreezing agent; about 0% to about 5 % stabilizing agent; about 0.1% to about 5.0 % Super-Wetting-spreading-penetrating agent; 0.01 to 10% pH stabilizer about 00.1 to 5% binding agent ; about 0.01% to 5 % pH modifier; and 0.01% to 5.0 % coloring agent.

3. The insecticidal composition as claimed in claim 3, wherein the emulsifier is selected from the group comprising ethoxylated propoxylated alcohols, alkylphenolethoxylates, alkoxylatedtristyrylphenols, calcium dodecylbenzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylated ricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof.

4. The insecticidal composition as claimed in claim 3, wherein the dispersing agent is selected from the group comprising polymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate, sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700, butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenol ethoxylates and polyalkylene oxide derivative of a synthetic alcohol, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof.

5. The insecticidal composition as claimed in claim 3, wherein the wetting agent is selected from the group comprising sodium alkyl naphthalene sulfonate, alpha olefin sulfonates, disodium laureth sulfosuccinate, diisodecyl sodium sulfosuccinate, alkyl sulfosuccinic monoesters, dioctyl sulfosuccinate sodium salt, sulfonic acids C14-16-alkane hydroxy and C14-16-alkene sodium salts, polyoxyethylene sorbitan monooleate, polyoxyethylene ether or mixtures thereof.

6. The insecticidal composition as claimed in claim 3, wherein the antifoaming agent is selected from the group comprising polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof.

7. The insecticidal composition as claimed in claim 3, wherein the rheology modifier is selected from the group comprising precipitated silica, fumed silica, modified fumed silica, bentonite, hydroxymethyl cellulose, carboxymethyl cellulose, xanthan gum, thickening silica, hydrated clay minerals, magnesium aluminium silicates, organic derivative of hectorite clay, hydrophobic fumed silica, polyvinylpyrrolidone (PVP) or mixture thereof.

8. The insecticidal composition as claimed in claim 3, wherein the solvent is selected Demineralized (DM) water, N-alkyl-pyrrolidone, oil medium selected from the group comprising, esterified fatty acids selected from methyl and/or ethyl ester of vegetable oil such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester or mixtures thereof.

9. The insecticidal composition as claimed in claim 3, wherein the binding is selected from the group comprising aluminium silicate, precipitated silica, fumed silica, diatomaceous earth, glucose polymers: amylose and amylopectin, a branched form, lactose anhydrous, silicic acid, sodium lignosulonate, modified phyllosilicate, either alone or mixtures thereof.
10. The insecticidal composition as claimed in claim 3, wherein inert carrier is selected from the group comprising kaolin, china clay, dextrin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof.

11. The insecticidal composition as claimed in claim 3, wherein the antifreezing agent is selected from the group comprising ethylene glycol, 1,2-propanediol, propane-1,2,3-triol, urea or mixtures thereof.

12. The insecticidal composition as claimed in claim 3, wherein the stabilizing agent is selected from the group comprising epoxidized soyabean oil, butylated hydroxy toluene, ethylenediaminetetraacetic acid, sodium benzoate, etc. or mixtures thereof.

13. The insecticidal composition as claimed in claim 3, wherein the pH modifier is selected from the group comprising sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, tartaric acid, hydrochloric acid or mixtures thereof.

14. The insecticidal composition as claimed in claim 3, wherein the pH stabilizers is selected from the group comprising sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, citric acid, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, hydrochloric acid.

15. The insecticidal composition as claimed in claim 3, wherein the Biocide selected from the group comprising of 1,2-benzisothiazolin-3-one, formaldehyde, dipropyl glycol solution of 1,2-benzisothiazolin-3-one or mixtures thereof.
16. The insecticidal composition as claimed in claim 3, wherein the Wetting cum dispersing agent is selected from the group comprising of, but not limited to non-ionic proprietary surfactant blend alkylphenol ethoxylates or polyoxyethylene sorbitan esters, lignosulfonates, Mixture of tristyrylphenol ethoxylates andpolyalkylene oxide derivative of a synthetic, alcohol sodium salt of naphthalene sulfonate condensates, tristyrylphenol ethoxylates.

17. The insecticidal composition as claimed in claim 3, wherein the Super-Wetting-spreading-penetrating agent is blend of methylated seed oil-organic silicone compound may be selected from methylated seed oil-polyalkyleneoxide modified trisiloxane, methylated seed oil-polyalkyleneoxide modified polydimethylsiloxane, methylated seed oil-20 trisiloxane ethoxylate, Polyalkyleneoxide modified heptamethyltrisiloxane methylated seed oil-polyoxyethylene methyl polysiloxane, methylated seed oil-polyether polymethyl siloxane copolymer, methylated seed oil-polyether modified polysiloxane.

18. The insecticidal composition as claimed in claim 3, wherein the coloring agent is selected from dye, pigment or mixtures thereof such as Triaryl methane acid blue, 3-hydroxy-N-(2-methylphenyl)-4-[(E)-(2,4,5-trichlorophenyl)diazinyl]-2-naphthamideand Acid Brilliant Green.

19. The process of preparation of an insecticidal composition as claimed in claim 1, the process comprising: preparation of the said composition with agriculturally acceptable excipients.