Description:FIELD OF THE INVENTION
The present disclosure relates to the field of insecticidal compositions. The present disclosure in particular relates to insecticidal compositions comprising Cyclaniliproleand Cartap hydrochloride that exhibits insecticidal properties. The present disclosure further relates to the process of preparation of said composition and uses thereof.
BACKGROUND OF THE INVENTION
Invertebrate pests and in particular insects, arthropods and nematodes often destroy growing and harvested crops and attack wooden dwelling and commercial structures, thereby causing large economic loss to the food supply and to property. The control of invertebrate pests in forestry, greenhouse crops, ornamentals, nursery crops, stored food and fiber products, livestock, household, turf, wood products, and public and animal health is also important.
Nowadays, insecticides, miticides, and microbicides are developed for controlling various pests, such as horticulture pests, forest pests, and sanitary pests, and a variety of such chemicals are prepared for actual uses. However, by using these chemicals for long years, pests have acquired insecticide resistances, and pathogens have acquired microbicide resistances. Accordingly, the cases that are hard to be controlled by conventional chemicals have been increasing in recent years. Also, some of such chemicals are highly toxic, and gradually disturbing ecosystems by remaining in environments for long years. Accordingly, developments of novel chemicals that are low toxic and low persistent, in addition to having excellent pest control effects, have always been expected.
It is though known well that in the practical process of agricultural production, the use of pesticides brings great benefits to the agricultural production and also brings certain problems, wherein the generation of pest drug resistance is one of the main problems. However, in this scenario if a certain preparation is singly used for a long time, the pests are easy to generate different degrees of drug resistance, and the dosage of pesticides can be increased, the environment is polluted, and the cost is increased.
Therefore, in view of the above drawbacks, the control of invertebrate pests is extremely important in achieving high crop efficiency. Damage by invertebrate pests to growing and stored agronomic crops can cause significant reduction in productivity and thereby result in increased costs to the consumer. Many products are commercially available for these purposes, but the need continues for new compounds that are more effective, less costly, less toxic, environmentally safer or have different sites of action.
Reference may be made to CA2953903C, wherein it discloses an insecticide, miticide, nematicide, molluscicide, disinfectant, or bactericide composition containing one or two substances selected from 4-[5-(3,5-dichlorophenyl)-4,5-dihydro-5-(trifluoromethyl)isoxazole-3-yl]-2-methyl-N-[2- oxo-2-[(2,2,2-trifluoroethyl)amino]ethyl]benzamide and (Z)-4-[5-(3,5-dichlorophenyl)-5-trifluoromethyl-4,5-dihydro-isoxazol-3-yl]-N-(methoxyiminomethyl)-2-methylbenzoic acid amide, and one or more substances selected from known insecticide, miticide, nematicide, molluscicide, disinfectant, or bactericide compounds.
Reference may be made to CA2923814C wherein it discloses acertain substituted bicyclic azoles, their N-oxides, salts and compositions suitable for agronomic and nonagronomic uses, and methods of their use for controlling invertebrate pests such as arthropods in both agronomic and nonagronomic environments.
In view of above situations, and as results of intensive studies there is absolutely a need to develop insecticide composition that show excellent pest control activities, and show little adverse impact to mammals, fishes, and non-target organisms such as natural enemies and beneficial insectsto provide a composition with high synergistic action, to provide a combination where the dosage of the active ingredients is reduced as compared to the individual recommended dosage, to avoid excess loading of the toxicant to the environment and to negligibly impact environmental safety.
Cyclaniliproleis a compound with CAS registration number 1031756-98-5. Itis a new anthranilic diamide insecticide that is proposed for agricultural use. Like other diamide insecticides such as chlorantraniliprole and cyantraniliprole, cyclaniliprole is a ryanodine receptor modulatorandknown to impair nerve and muscle function which leads to rapid feeding cessation, regurgitation, lethargy and contractile paralysis of insects.
Cyclaniliproleis well known with IUPAC name 5-bromo-N-[2-bromo-4-chloro-6-(1-cyclopropylethylcarbamoyl)phenyl]-2-(3-chloropyridin-2-yl) pyrazole-3-carboxamide having chemical structure as:

CYCLANILIPROLE
Cartap hydrochlorideis a compound with CAS registration number 15263-52-2. It isa derivative of nereistoxin, a naturally occurring insecticidal substance isolated from the marine segmented worms Lumbrinereisheteropoda and Lumbrinereisbrevicirra. Cartap hydrochloride is a highly effective, broad-spectrum, low toxic and low residual insecticide, causing paralysis by cholinergic blocking action on the central nervous system of insect. It is a systemic insecticide with stomach and contact action. Insects discontinue feeding and die of starvation. Cartaphydrochloride is used against a relatively broad spectrum of insects, e.g., Lepidoptera, Coleoptera, Diptera and Hemiptera.
Cartap hydrochloride is well known with IUPAC name s,s'-(2-dimethylaminotrimethylene) bis(thiocarbamate) hydrochloride] having chemical structure as

CARTAP HYDROCHLORIDE
It has been found that, as a solution to the above-mentioned problems, a combination comprising Cyclaniliproleand Cartap hydrochloride provides an effective composition in controlling a wide variety of undesired pathogenic microorganisms.
OBJECTS OF THE INVENTION
It is an object of the present invention to provide a novel, stable, synergistic, insecticidal composition of Cyclaniliprole and Cartap hydrochloride demonstrating high efficacy and high selectivity.
It is yet another object of the present invention to provide insecticide, miticide, nematicide, molluscicide, disinfectant, or bactericide composition, and a pest control method that has broad spectrum activity and can be used in a wide variety of agricultural crops.
It is yet another object of the present invention to provide the insecticidal composition with enhanced bio efficacy at reduced dosage as compared to individual insecticides when used in isolation.
It is another object of the present invention to provide a novel and effective insecticidal composition for controlling the harmful pests and insects in plants.
It is another object of the present invention to provide a novel and effective synergistic insecticidal composition which can be easily formulated.
It is yet another object of the present invention to provide the insecticidal composition that result in good enhancement in crop yield.
It is yet another object of the present invention to provide the insecticidal composition that is environmentally safe and non-phytotoxic.
SUMMARY OF THE INVENTION
In an aspect, an insecticidal composition, comprising:0.1% to about 30.0% w/w Cyclaniliprole by weight of the composition; about 0.1% to about 70.0% w/w Cartap hydrochloride by weight of the composition; and agriculturally accepted excipient(s) .
In yet another aspect, the present invention provides a synergistic composition comprising Cyclaniliprole and Cartap hydrochloride, the composition possesses insecticidal activity.
In a further aspect, the present invention provides a process for effective control of various insects in plants.
In another aspect, the agriculturally accepted excipients are selected from the group comprising group comprising an emulsifier; dispersing agent; wetting agent; antifoaming agent; rheology modifier; solvent; pH modifier; inert carrier; biocide; an antifreezing agent; binding agent pH; stabilizers stabilizing agent;and coloring agent wherein the composition comprises- about 0.1% to about 20.0% emulsifier; about 0.1% to about 20.0% dispersing agent; about 0.1% to about 15.0% wetting agent; about 0.01% to about 5.0% antifoaming agent; about 0.01% to about 20.0% rheology modifier; about 0% to about 95.0% solvent;; about 0% to 90.0 % inert carrier; ;about 0 to 95% sovents; about 0% to 3 % biocide; about 0% to 15 % antifreezing agent; 0.01 to 10% pH stabilizers ; 0.1% to 5% Super-Wetting-spreading-penetrating agent;;0% to 5 % stabilizing agent; 0% to 5 % pH modifier; and 0.01% to 5.0 % coloring agent.
In another aspect, the emulsifier is selected from the group comprising ethoxylated propoxylated alcohols, alkylphenolethoxylates, alkoxylatedtristyrylphenols, calcium dodecylbenzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylated ricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof.
In another aspect, the dispersing agent is selected from the group comprising polymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate, sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700, butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenol ethoxylates and polyalkylene oxide derivative of a synthetic alcohol, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof.
In another aspect, the wetting agent is selected from the group comprising sodium alkyl naphthalene sulfonate, alpha olefin sulfonates, disodium laureth sulfosuccinate, diisodecyl sodium sulfosuccinate, alkyl sulfosuccinic monoesters, dioctyl sulfosuccinate sodium salt, sulfonic acids C14-16-alkane hydroxy and C14-16-alkene sodium salts, polyoxyethylene sorbitan monooleate, polyoxyethylene ether or mixtures thereof.
In another aspect, wherein the antifoaming agent is selected from the group comprising polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof.
In another aspect, the rheology modifier is selected from the group comprising precipitated silica, fumed silica, modified fumed silica, bentonite, hydroxymethyl cellulose, carboxymethyl cellulose, xanthan gum, thickening silica, hydrated clay minerals, magnesium aluminium silicates, organic derivative of hectorite clay, hydrophobic fumed silica, polyvinylpyrrolidone (PVP) or mixture thereof.
In another aspect, the solvent is selected from the group comprising Demineralized (DM) water, N-alkyl-pyrrolidone, oil medium selected from the group comprising, esterified fatty acids selected from methyl and/or ethyl ester of vegetable oil such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester or mixtures thereof.
In another aspect, the inert carrier is selected from the group comprising kaolin, china clay, dextrin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof.
In another aspect,the biocide is selected from the group comprising 1,2-benzisothiazolin-3-one, formaldehyde, dipropyl glycol solution of 1,2-benzisothiazolin-3-one or mixtures thereof.
In another aspect, the antifreezing agent is selected from the group comprising ethylene glycol, 1,2-propanediol, propane-1,2,3-triol, urea or mixtures thereof.
In another aspect the stabilizing agent is selected from the group comprising epoxidized soyabean oil, butylated hydroxy toluene, ethylenediaminetetraacetic acid, sodium benzoate, etc. or mixtures thereof.
In another aspect, the pH modifier is selected from the group comprising sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, tartaric acid, hydrochloric acid or mixtures thereof.
In another aspect, the coloring agent is selected from Triaryl methane acid blue, 3-hydroxy-N-(2-methylphenyl)-4-[(E)-(2,4,5-trichlorophenyl)diazinyl]-2-naphthamide and Acid Brilliant Green.
In another aspect, Super-Wetting-spreading-penetrating agent is blend of methylated seed oil-organic silicone compound may be selected from methylated seed oil-polyalkyleneoxide modified trisiloxane, methylated seed oil-polyalkyleneoxide modified polydimethylsiloxane, methylated seed oil-20 trisiloxane ethoxylate, Polyalkyleneoxide modified heptamethyltrisiloxane methylated seed oil-polyoxyethylene methyl polysiloxane, methylated seed oil-polyether polymethyl siloxane copolymer, methylated seed oil-polyether modified polysiloxane.
In another aspect, the pH stabilizers sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, citric acid, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, hydrochloric acid or mixtures thereof.
In yet another embodiment of the present invention, the invention further provides the process for preparation of said compositionthat can be one or more of capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG) Emulsifiable water-in-oil (EO), Emulsifiable powder (EP), Emulsifiable for seed treatment (ES), Emulsifiable oil-in-water (EW), flowable concentrate for seed treatment (FS), Suspension Concentrate (SC), Suspo-emulsion (SE), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG) and Wettable powders (WP), a mixed formulation of CS and SC (ZC), soluble liquid (SL).
Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments.
DETAILED DESCRIPTION OF THE INVENTION
hose skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, composition, formulation and compounds referred to or indicated in this specification, individually or collectively, and all combinations of any two or more of said steps or features. Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of the ordinary skills in the art to which the invention belongs. For further guidance, term definitions may be included to better appreciate the teaching of the present invention.
As used herein, the term “plant” refers to any plant or part thereof including serial and subterranean parts of the plant. It is contemplated that the parts of the plant may be for example, flowers, fruits or vegetables, shoots, leaves, needles, stalks, stems, fruiting bodies, seeds also roots and that parts of the plants may or may not be attached to the remainder of the plant.
The term “locus” of a plant as used herein is intended to embrace the place on which the plants are growing, where the plant propagation materials of the plants are sown or where the plant propagation materials of the plants will be placed into the soil. The term “plant propagation material” is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil.
It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components or groups thereof. The term "insects" as used herein, includes all organisms in the class "Insecta." The term "insecticidal" as used herein, refers to the ability of a pesticide to increase mortality or inhibit growth rate of insects.
The terms “weight percent”, “wt%”, “percent by weight”, “% by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the formulation and multiplied by 100. It is understood that, as used here, “percent “, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc.
The term “synergist” as used herein refers to a material which enhances the activity of other materials such as insecticides so that the overall activity of the formulation is greater than the sum of the individual ingredients. Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.
Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.
The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
As used herein, the term “additive(s)” or "auxiliary agent(s)" or “agrochemically acceptable carrier(s)” can be used interchangeably and refers to inert substances which are commonly used asdiluent, to provide stability or to increase the activity profile of the composition or formulation with or without having agrochemical activity or direct effect on the undesired phytopathogenic fungi and/or microorganisms.
As used herein, the term "agrochemically acceptable salts" are typically acid addition salts of inorganic or organic acids, preferably of hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, perchloric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, malonic acid, toluenesulfonic acid or benzoic acid.
As used herein, the term “additive(s)” or "auxiliary agent(s)" or “agriculturally acceptable carrier(s)” can be used interchangeably and refers to inert substances which are commonly used as diluent, to provide stability or to increase the activity profile of the composition or formulation with or without having agrochemical activity.
As used herein, the term “lower” or “minimal” or “minimum” can be used interchangeably and refers to the optimum concentration of active or inactive ingredients of formulation to achieve the expected efficacy.
As used herein, the term "effective amount" means the amount of the active substances in the compositions to achieve an observable effect on growth, including the effects of necrosis, death retardation, prevention, and removal, destruction, or otherwise diminishing the occurrence and activity of the target organism. The effective amount can vary for the various compositions used in the present invention. An effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like.
As used herein, the term “composition” or "formulation" can be used interchangeably, unless stated otherwise, is meant to encompass, and are not limited to, compositions or formulations containing the combination of cyclaniliprole and cartap hydrochloride.
The present disclosure relates to insecticidal composition. Particularly, the present disclosure provides the insecticidal composition including cyclaniliprole and cartap hydrochloride and agriculturally acceptable excipient(s) that exhibits synergistic properties.
Mechanism of action:
Cyclaniliprole is a selective activation of the ryanodine receptor (RyR) in the sarcoplasmic reticulum of target insect pests. Once exposed, through contact or ingestion, Cyclaniliprole attaches to the insect RyR inducing the uncontrolled release of calcium stores present in muscle cells.
Cartap hydrochloride is an insecticide that works by blocking the central nervous system (CNS) of insects, causing paralysis and starvation.
Technical effect:
The present disclosure is based on the surprisingly an unexpected finding by the Applicant that composition including Cyclaniliprole and Cartap hydrochloride and agriculturally acceptable excipient(s) that aids in reducing dosage of the overall compositions and formulation and consequently aids in reducing phytotoxicity to nil.
Bio efficiency and phytotoxicity results are well elaborated and provided in examples later.
The present invention thus provides an agrochemical composition comprising Cyclaniliproleas the first active ingredient; Cartap hydrochloride as the second active ingredient; and agriculturally acceptable excipient(s). In the composition- Cyclaniliprole is present in an amount ranging from 0.1% to 30.0% % w/w, Cartap hydrochloride is present in an amount ranging from 0.1% to 75.0% % w/w and agriculturally acceptable excipient(s) .
In a preferred embodiment, Cyclaniliproleis present in an amount4.0%w/w of the insecticidal composition and Cartap hydrochloride is present in an amount of 45%w/w of the insecticidal composition.
In another aspect, the agriculturally accepted excipients are selected from the group comprising group comprising an emulsifier; dispersing agent; wetting agent; antifoaming agent; rheology modifier; solvent; pH modifier; inert carrier; biocide; an antifreezing agent; pH Stabilizers; Super-Wetting-spreading-penetrating agent; stabilizing agent; and coloring agent wherein the composition comprises- about 0.1% to about 20.0% emulsifier; about 0.1% to about 20.0% dispersing agent; about 0.1% to about 15.0% wetting agent; about 0.01% to about 5.0% antifoaming agent; about 0.01% to about 20.0% rheology modifier;about 0 to 95% sovents ; about 0% to about 95.0% solvent; about 0% to 90.0 % inert carrier; about 0% to 3 % biocide; about 0% to 15 % antifreezing agent; 0% to 5 % stabilizing agent; 0.01 to 10% pH Stabilizers; 0% to 5% Super-Wetting-spreading-penetrating agent; 0% to 5 % pH modifier; and 0.01% to 5.0 % coloring agent.
In another embodiment, the emulsifier is selected from the group comprising ethoxylated propoxylated alcohols, alkylphenolethoxylates, alkoxylatedtristyrylphenols, calcium dodecylbenzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylated ricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof.
In another embodiment, the dispersing agent is selected from the group comprisingpolymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate, sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700, butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenol ethoxylates and polyalkylene oxide derivative of a synthetic alcohol, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof.
In another embodiment, the wetting agent is selected from the group comprising sodium alkyl naphthalene sulfonate, alpha olefin sulfonates, disodium laureth sulfosuccinate, diisodecyl sodium sulfosuccinate, alkyl sulfosuccinic monoesters, dioctyl sulfosuccinate sodium salt, sulfonic acids C14-16-alkane hydroxy and C14-16-alkene sodium salts, polyoxyethylene sorbitan monooleate, polyoxyethylene ether or mixtures thereof.
In another embodiment, the antifoaming agent is selected from the group comprising polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof.
In another embodiment, the rheology modifier is selected from the group comprising precipitated silica, fumed silica, modified fumed silica, bentonite, hydroxymethyl cellulose, carboxymethyl cellulose, xanthan gum, thickening silica, hydrated clay minerals, magnesium aluminium silicates, organic derivative of hectorite clay, hydrophobic fumed silica, polyvinylpyrrolidone (PVP) or mixture thereof.
In another embodiment, the solvent is selected from the group comprising Demineralized (DM) water, N-alkyl-pyrrolidone, oil medium selected from the group comprising, esterified fatty acids selected from methyl and/or ethyl ester of vegetable oil such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester or mixtures thereof.
In another embodiment, inert carrier is selected from the group comprising kaolin, china clay, dextrin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof.
In another embodiment, the anti freezing agent is selected from the group comprising ethylene glycol, propane-1,2-diol, propane-1,2,3-triol, urea or mixtures thereof.
In another embodiment, the Super-Wetting-spreading-penetrating agent is blend of methylated seed oil-organic silicone compound may be selected from methylated seed oil-polyalkyleneoxide modified trisiloxane, methylated seed oil-polyalkyleneoxide modified polydimethylsiloxane, methylated seed oil-20 trisiloxane ethoxylate, Polyalkyleneoxide modified heptamethyltrisiloxane methylated seed oil-polyoxyethylene methyl polysiloxane, methylated seed oil-polyether polymethyl siloxane copolymer, methylated seed oil-polyether modified polysiloxane.
In another embodiment, the stabilizing agent is selected from the group comprising epoxidized soyabean oil, butylated hydroxy toluene, ethylenediaminetetraacetic acid, sodium benzoate, etc. or mixtures thereof.
In another embodiment, the pH modifier is selected from the group comprising sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, tartaric acid, hydrochloric acid or mixtures thereof.
In another embodiment, the coloring agent is selected from dye, pigment or mixtures thereof such as Triaryl methane acid blue, 3-hydroxy-N-(2-methylphenyl)-4-[(E)-(2,4,5-trichlorophenyl)diazinyl]-2-naphthamideand Acid Brilliant Green or mixtures thereof.
In an embodiment, the composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules(GG), dry flowables (DF), aqueous Suspo-emulsions (SE), capsule suspensions (CS) and micro granules (MG). In a preferred embodiment, the composition is in the form of suspension concentrates (SC), water-dispersible granule (WDG) and wettable powder (WP).
In an embodiment, the insecticidal composition is formulated in a form selected from the group comprising water-dispersible granule (WDG), a wettable powder (WP), a granule (GR), an oil-dispersion (OD), etc.
In yet another embodiment, the combination or the composition of the present invention is particularly important for controlling a multitude of undesired pathogenic microorganisms, on various cultivated plants or plant parts, such as cereals, e.g. paddy, wheat, rye, barley, triticale, oats or rice; beet, e.g. sugar beet or fodder beet; fruits, such as pomes, stone fruits or soft fruits, e.g. apples, pears, plums, peaches, almonds, cherries, strawberries, raspberries, blackberries or gooseberries; leguminous plants, such as lentils, peas, alfalfa or soybeans; oil plants, such as rape, mustard, olives, sunflowers, coconut, cocoabeans, castor oil plants, oil palms, ground nuts or soybeans; cucurbits, such as squashes, cucumber or melons; fiber plants, such as cotton, flax, hemp or jute; citrus fruit, such as oranges, lemons, grapefruits or mandarins; vegetables, such as spinach, lettuce, asparagus, cabbages, carrots, onions, tomatoes, potatoes, cucurbits or paprika; lauraceous plants, such as avocados, cinnamon or camphor; energy and raw material plants, such as corn, soybean, rape, sugar cane or oil palm; corn; tobacco; nuts; coffee; tea; bananas; vines (table grapes and grape juice grape vines); hop; turf; sweet leaf (also called Stevia); natural rubber plants or ornamental and forestry plants, such as flowers, shrubs, broadleaved trees or evergreens, e.g. conifers; and on the plant propagation material, such as seeds, and the crop material of these plants.
In another embodiment of the present invention, the invention further provides the process for preparation of the said composition wherein, the said composition can be one or more of as suspension concentrate (SC), wettable granules (WG), wettable powder (WP), a water dispersible granule (WDG), a water dispersible tablet (WT), an ultra-low volu (ULV) liquid (UL), an ultra-low volume (ULV) suspension (SU), a water soluble powder (SP), a suspo-emulsion (SE), granule (GR), an emulsifiable granule (EG), an oil-in-water or water in oil emulsion (EW), an emulsifiable concentrate (EC), a micro-emulsion (ME), an oil dispersion (OD), a capsule suspension (CS), a dustablepowder (DP) or an aerosol (AE).
In another preferred embodiment of the invention, the yield of the plants treated according to the method of the invention, is increased synergistically. According to the present invention, "increased yield" of a plant, in particular of an agricultural, silvicultural and/or horticultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the mixture according to the invention.
Increase in yield of treated plants means increased plant weight, increased plant height, increased biomass such as higher overall fresh weight (FW), increased number of flowers per plant, higher grain yield, more tillers or side shoots (branches), larger leaves, increased shoot growth, increased protein content, increased oil content, increased starch content, increased pigment content, Increase in yield due to protection against Insect-pest damage and fungal diseases, Increase in yield due to plant growth regulation, check vegetative growth and increase in reproductive parts of plant, Increase in yield due to more number of tillers, more branches and sub branches, more number of fruits, flowers, and grains size, Increase plant vigor, Increase tolerance to insect-pests and fungal damage, Increase tolerance to the weather stress, moisture stress and heat stress, Prevents lodging in susceptible plants (lodging due to biotic and abiotic factors, like heavy rains, winds, insects and diseases damage.
While the foregoing description discloses various embodiments of the disclosure, other and further embodiments of the invention may be devised without departing from the basic scope of the disclosure. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
Examples-:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 1: Preparation of Insecticidal composition as Water dispersible granule (WDG) formulation:
As per some specific exemplary embodiments, Water dispersible granule (WDG) formulation was prepared with composition as per Table 1 as below:

Table 1. Insecticidal composition of Cyclaniliprole and Cartap hydrochloride as water dispersible granule (WDG) formulation
Component Quantity
(% w/w) Function
Cyclaniliproletechnical 4.00 Active ingredient
Cartap Hydrochloride technical 45.00 Active ingredient
Sodium lauryl sulphate 1.00 Wetting agent
Polyoxyethylene sorbitan monooleate 1.50 Wetting agent
Polyoxyethylene Ether 1.50 Wetting agent
Sodium salt of naphthalene sulfonate condensate 1.50 Dispersing agent
Polydimethylsiloxane emulsion 0.50 Antifoaming agent
Tartaric acid 3.00 pH modifier
Citric acid 0.50 pH modifier
Polyvinylpyrrolidone 2.50 rheology modifier
Ammonium sulphate 1.00 Inert carrier
Dextrin 7.00 Inert carrier
Precipitated silica 1.00 Inert carrier
Kaolin Balance to 100 Inert carrier

Preparation of insecticidal composition of Cyclaniliprole and Cartap Hydrochloride water dispersible granules:
The required amounts of active ingredient Cyclaniliprole, and other components like sodium lauryl sulphate, sodium salt of naphthalene sulfonate condensate, dextrin, tartaric acid, citric acid, polyvinylpyrrolidone, ammonium sulphate, precipitated silica and kaolin were blended with suitable equipmentlikein homogenizer. This was first part of the process.
Following the above, the mixture was milled with appropriate equipment to form milled Cyclaniliprole pre-mix. To this the obtained milled Cyclaniliprole pre-mix required amount of Cartap hydrochloride, polyoxyethylene sorbitan monooleate, polyoxyethylene ether and polydimethylsiloxane emulsion were blended together to obtain another mixture.
From the above mixture, then dough was prepared by adding appropriate amount of water. The dough was extruded with the help of a suitable extruder. The extrudes were dried with appropriate equipment. The dried extrude was sieved through a sieve to form insecticidal composition of Cyclaniliprole and Cartap Hydrochloride water dispersible granules.
Example 2: Preparation of Insecticidal composition as Wettable powder (WP) formulation:
As per some specific exemplary embodiments, Wettable powder (WP) formulation was prepared with composition as per Table 2 as below:
Table 2. Insecticidal composition of Cyclaniliprole and Cartap hydrochloride as Wettable powder (WP) formulation
Component Quantity
(% w/w) Function
Cyclaniliprole technical 4.00 Active ingredient
Cartap Hydrochloride technical 45.00 Active ingredient
Sodium lauryl sulphate 1.00 Wetting agent
Polyoxyethylene sorbitan monooleate 1.50 Wetting agent
Polyoxyethylene Ether 1.50 Wetting agent
Sodium salt of naphthalene sulfonate condensate 1.50 Dispersing agent
Polydimethylsiloxane emulsion 0.50 Antifoaming agent
Tartaric acid 3.00 pH modifier
Citric acid 0.50 pH modifier
Ammonium sulphate 1.00 Inert carrier
Dextrin 7.00 Inert carrier
Precipitated silica 3.00 Inert carrier
Kaolin Balance to 100 Inert carrier

Preparation of insecticidal composition of Cyclaniliprole and Cartap Hydrochloride wettable powder:
The required amounts of Cyclaniliprole technical, sodium lauryl sulphate, sodium salt of naphthalene sulfonate condensate, dextrin, tartaric acid, citric acid, ammonium sulphate, precipitated silica and kaolin were blended with suitable equipment like homogenizer.
The obtained mixture was milled with appropriate equipment to form milled Cyclaniliprole pre-mix. To this obtained milled Cyclaniliprole pre-mix required amount of Cartap hydrochloride technical, polyoxyethylene sorbitan monooleate, polyoxyethylene ether and polydimethylsiloxane emulsion were blended with suitable equipment to form insecticidal composition of Cyclaniliprole and Cartap Hydrochloride wettable powder.
Example 3: Preparation of Insecticidal composition as Granules (GR)formulation:
As per some specific exemplary embodiments, Granules (GR) formulation was prepared with composition as per Table 3 as below:

Table 3. Insecticidal composition of Cyclaniliprole and Cartap hydrochloride as Granules (GR) formulation
Component Quantity
(% w/w) Function
Cyclaniliprole technical 0.40 Active ingredient
Cartap Hydrochloride technical 4.50 Active ingredient
Polyoxyethylene sorbitan monooleate 1.50 Wetting agent
Polyoxyethyleneether 1.50 Wetting agent
Dye 0.10 Coloring agent
Citric acid 0.50 pH modifier
Precipitated silica 0.30 Inert carrier
China clay 4.00 Inert carrier
White sand Balance to 100 Inert carrier

Preparation of insecticidal composition of Cyclaniliprole and Cartap Hydrochloride granules:
The granules for the present composition were prepared by taking required amount of white sand that was added in appropriate mixing vessel.
To this polyoxyethylene sorbitan monooleate and polyoxyethylene ether was added under mixing. Then Cyclaniliprole (pre-grinded), Cartap Hydrochloride, dye, citric acid, china clay and precipitated silica were added and blended with for sufficient time.
The obtained material was then sieved with suitable sieve to obtain insecticidal composition of Cyclaniliprole and Cartap Hydrochloride granules.

Example 4: Preparation of Insecticidal composition as Oil Dispersion (OD) formulation:
As per some specific exemplary embodiments, Oil Dispersion (OD) formulation was prepared with composition as per Table 4 as below:
Table 4. Insecticidal composition of Cyclaniliprole and Cartap hydrochloride as Oil Dispersion (OD) formulation
Component Composition (%w/w) Remark
Cyclaniliprole 4.50 Active Ingredient
Cartap hydrochloride 45.00 Active Ingredient
Castor oil ethoxylate (10 mole) 6.00 Emulsifier
Calcium dodecylbenzenesulfonate 2.00 Emulsifier
Mixture of fatty acid polyethylene glycol ester 4.00 Emulsifier
Polymeric ester dispersant 0.5 Dispersing agent
Ethoxylated polyarylphenol phosphate ester 0.5 Dispersing agent
Propane-1,2,3-triol 2.00 Antifreezing Agent
Polyalkyleneoxide modified heptamethyltrisiloxane 1.00 Super-Wetting-spreading-penetrating agent
Polydimethylsiloxane 0.30 Antifoaming agent
Fumed silica 1.50 Rheology modifier
Citric acid 0.10 pH stabilizer
Sodium Benzoate 1.00 Stabilizing agent
Bentonite clay 1.50 Rheology modifier
Methyl ester of vegetable oil Balance to 100 Solvent

Process of preparation of insecticidal composition of Cyclaniliprole and Cartap Hydrochloride oil dispersion (OD)formulation:
In the process obtained for preparing Oil dispersion formulation, the respective quantities ofmethyl ester of vegetable oilconstituents namely, polyalkyleneoxide modified heptamethyltrisiloxane, polydimethylsiloxane, propane-1,2,3-triol, castor oil ethoxylate (10 mole),calcium dodecylbenzenesulfonate, ethoxylated polyarylphenol phosphate ester, mixture of fatty acid polyethylene glycol ester and polymeric ester dispersant as mentioned in Table 4were added into the clean pre-mixing vessel fitted with the homogenizer and all the ingredients were mixed for sufficient time with the homogenizer to obtain a mixture.
The active ingredients, Cyclaniliprole, and Cartap hydrochloride as well as pH stabilizer and stabilizing agent were added to the obtained mixture and continued to mix for sufficient time with the homogenizer to obtain a slurry.
The slurry was then passed through jacketed bead mill with chilled water circulation for particle size reduction to obtain a milled slurry of desirable particle size. The milled slurry was thancollected into post mixing vessel fitted with the stirrer. To milled slurry bentonite clayand fumed silica were added under stirring into the post mixing vessel and continued to mix for sufficient time up to proper mixing to form Insecticidal Oil Dispersion (OD) formulation.
Example -5: Stability Data
Stability Study (Accelerated Storage test and low-temperature storage test)
According to the FAO/WHO manual, the “accelerated storage test” is considered as an indicative of product stability. That is, accelerated storage test data provides an indication that the product is stable for at least two years at ambient temperature. Further, the FAO/WHO manual indicates storage at 54 ± 2°C for 14 days as the default test conditions. Further, the FAO/WHO manual indicates low-temperature storage at 0 ± 2°C for 7 days.

The “ambient” is the sample at the room temperature which is before subjecting to the accelerated storage test.
The “accelerated storage” is the sample after subjecting the sample to accelerated storage tests at 54±2°C for 14 days.
The insecticidal formulation composition of examples 1 - 3 were found stable after accelerated stability study at 54 ± 2° C for 14 days.
The insecticidal formulation composition of example 4 was found stable after accelerated stability study at 54 ± 2° C for 14 days and low temperature at 0 ± 2°C for 7 days.
Example 6-
Evaluation of Bio efficacy of an Insecticidal Composition and thereof (Cyclaniliprole + Cartap hydrochloride) on paddy against leaf folder and stem borer-
In the experiment, many field trials were conducted to evaluate the effect of different insecticidal formulations of combination treatments, solo formulations of Cyclaniliprole 10% DC, Cartap Hydrochloride 50% SP against leaf folder (Cnaphalocrocismedinalis) and stem borer (Scirpophagaincerulas) of paddy.
Details of the experiment:
The experiment was laid out in Randomized Block Design (RBD) with three replications. Throughout the cropping period, all the recommended agronomic practices were followed. Various insecticidal compositions were applied on the paddy crop to evaluate the efficacy on rice leaf folder and stem borer.
Application was done during the vegetative phase when the insect pests population reached economic threshold level (ETL) which is 10% leaf damage and 10% dead heart in case of rice leaf folder and stem borer, respectively. Observations were recorded randomly from each replication (10 hills per replication) on 5th, 10th and 15th day after spray (DAS).
Rice leaf folder
For rice leaf folder, 10 hills were randomly tagged in each replication. Total number of leaves and number of leaves damaged were counted. Leaf damage (%) was calculated as follows:
Number of damaged leaves in 10 hills
Leaf damage (%) = ------------------------------------------------ X 100
Total number of leaves in 10 hills
Rice stem borer
For rice stem borer, 10 hills were randomly tagged in each replication. Total number of tillers and number of dead hearts were counted from all the tagged plants. Dead hearts were pulled out after every observation from all the treatments including untreated check. Dead heart (%) was calculated as follows:
Number of dead hearts in infested hills
Dead heart (%) =------------------------------------------------------ X 100
Total no. of tillers observed in infested hills
Synergistic effect of the different insecticidal compositions
Synergistic effect was checked by using Colby’s method for ternary mixes. In the Colby’s method, for a given combination of two active components, E (expected efficacy) can be expressed as:

E = A + B –(AB)
100
Where, E = expected efficacy,
A and B = the efficacy of two active ingredients A and B at a given dose.
Synergy ratio (R) = Experimentally observed efficacy (O)
Expected efficacy (E)

If the synergism ratio (R) between observed and expected is >1 then synergy is exhibited, if R=1 then the effect is additive and if R<1 then the mix is antagonistic.
The experimental data was statistically analysed by Randomized Block Design (RBD) (One factor analysis) using OPSTAT HAU statistical software. The results are expressed as Mean ± SE (standard error) and data was statistically analysed by one-way Analysis of variance (ANOVA), with the level of significance set at par< 0.01.
Phytotoxicity observations
For the evaluation of phytotoxicity on paddy crop, observations were made by observing the temporary or long-lasting damage to the leaves if any viz., yellowing, wilting, necrosis, epinasty and hyponasty at 5, 10 and 15 DAS of the synergistic insecticidal composition of the present invention. Crop injury was observed on visual rating from 0-10 scale as presented in Table 5.

Table 5.Phytotoxicity rating scale
Rating Crop Injury (%) Description
0 - No symptoms
1 1-10 Slight discoloration
2 11-20 More severe, but not lasting
3 21-30 Moderate and more lasting
4 31-40 Medium and lasting
5 41-50 Moderately heavy
6 51-60 Heavy
7 61-70 Very heavy
8 71-80 Nearly destroyed
9 81-90 Destroyed
10 91-100 Completely destroyed

Table 6: Effect of different treatments on the incidence of leaf folder in paddy:

Rice (Oryza sativa L.) is one of the most important cereal crops of the World, providing major source of the food energy for more than half of the human population.Insect-pests infesting rice that can cause significant damage to the crop, leading to lower yields and economic losses.While in terms of insects more than 100 species of insect attacks on rice crop out of which twentycause severe damage and yield loss to the rice crop in the laterstage. Several insects feed on the rice crop, but yellow stem borer (Scirpophagaincerulas)and leaf folder (Cnaphalocrocismedinalis) are considered the most important rice pests. Yellow stem borer attacks the crop from the seedling stage to harvesting stage and thus causes complete loss of affected tillers.

Sr. No.
Treatment Compositions Dosage/ha Leaf damage (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS Colby Value 10 DAS Colby Value 15 DAS
1 Cyclaniliprole 10% DC 40 400 5.47 (13.45) 7.80 (16.15) 10.60 (18.89) 59.31 60.61 56.08 - -
2 Cartap hydrochloride 50% SP 500 1000 7.10 (15.43) 10.00 (18.42) 13.27 (21.34) 47.15 49.49 45.03 - -
3 Cyclaniliprole 10% DC +Cartap hydrochloride 50% SP 40 +500 400+1000 2.10 (8.32) 2.83 (9.62) 4.20 (11.81) 84.37 85.69 82.60 1.07 1.08
4 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 15 + 200 500 3.70 (11.06) 5.13 (13.02) 7.30 (15.66) 72.46 74.07 69.75 0.92 0.92
5 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 18 + 240 600 3.30 (10.43) 4.60 (12.37) 6.55 (14.76) 75.43 76.77 72.87 0.95 0.96
6 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 21 + 280 700 3.03 (9.99) 4.23 (11.83) 5.97 (14.12) 77.42 78.62 75.28 0.98 0.99
7 Untreated Check - - 13.43 (21.47) 19.80 (26.38) 24.13 (29.40) - - - - -
CD at 5% 1.95 2.02 1.97 - - - - -
SE (m) 0.62 0.65 0.63 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray

Result & Discussion:
a) Leaf folder
• Among all the treatments the tank-mix combinations of Cyclaniliprole 10% DC @ 40 gm a.i./ha and Cartap hydrochloride 50% SP @ 500 gm a.i./ha was observed with highest per cent reduction in incidence of leaf folder recorded with 84.37%, 85.69%, 82.60% control at 5th, 10th and 15th day after spray, respectively followed by Cyclaniliprole 3.0% + Cartap hydrochloride 40% @ 21 + 280 gm a.i./ha, Cyclaniliprole 3.0% + Cartap hydrochloride 40% @ 18 + 240 gm a.i./ha and Cyclaniliprole 3.0% + Cartap hydrochloride 40% @ 15 + 200 gm a.i./ha.
• All the doses of pre-mix formulation of Cyclaniliprole 3.0% + Cartap hydrochloride 40% applied @21 + 280 gm a.i./ha, 18 + 240 gm a.i./ha and 15 + 200 gm a.i./ha observed with 75.28%, 72.87% and 69.75% reduction over untreated control at 15th DAS, respectively.
• All the doses of Cyclaniliprole 3.0% + Cartaphydrochloride 40%showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (56.08%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (45.03%) against leaf folder of rice at 15th day after spray.

Among the treatments only tank-mix combination of Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha showed synergistic effect with the synergistic ratio 1.089 (>1) at 15 DAS. (Table 6).

Table 7: Effect of different treatments on the incidence of leaf folder in paddy

Sr. No.
Treatment Compositions Dosage/ha Leaf damage (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS 10 DAS 15 DAS
1 Cyclaniliprole 10% DC 40 400 6.67 (14.98) 9.17 (17.57) 12.11 (20.33) 57.45 59.26 54.06 - -
2 Cartap hydrochloride 50% SP 500 1000 8.00 (16.40) 11.33 (19.63) 14.13 (22.06) 48.94 49.63 46.40 - -
3 Cyclaniliprole 10%DC +Cartap hydrochloride 50% SP 40 +500 400+1000 2.63 (9.32) 3.27 (10.26) 4.60 (12.34) 83.19 85.48 82.55 1.07 1.09
4 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 20 + 225 500 4.03 (11.54) 5.60 (13.67) 7.53 (15.89) 74.26 75.11 71.43 0.94 0.94
5 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 24 + 270 600 2.13 (8.38) 2.53 (9.12) 3.60 (10.92) 86.38 88.74 86.35 1.11 1.14
6 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 28 + 315 700 2.00 (8.12) 2.33 (8.74) 3.37 (10.44) 87.23 89.63 87.23 1.12 1.15
7 Untreated Check - - 15.67 (23.27) 22.50 (28.28) 26.37 (30.87) - - - - -
CD at 5% 1.81 1.99 2.08 - - - - -
SE (m) 0.58 0.64 0.67 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray

Among all the doses of combination and individual treatments, Cyclaniliprole 4.0% andCartap hydrochloride 45% @ 28 and 315 gm a.i./ha was observed with highest per cent reduction in incidence of leaf folder recorded with 87.23% control at 15th DAS and found at par with Cyclaniliprole 4.0% andCartap hydrochloride 45% @ 24 and 270 gm a.i./ha (86.35%) and Cyclaniliprole 10% DC @ 40 gm a.i./ha andCartap hydrochloride 50% SP @ 500 gm a.i./ha (82.55%) followed by Cyclaniliprole 4.0% and Cartap hydrochloride 45% @ 20 + 225 gm a.i./ha (71.43%) at 15th DAS.

All the doses of Cyclaniliprole 4.0% + Cartap hydrochloride 45% showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (54.06%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (46.40%) against leaf folder of rice at 15th day after spray. Cyclaniliprole 4.0% +Cartap hydrochloride 45% @ 24 + 270 gm a.i./ha showed synergistic effect with the synergistic ratio1.146 (>1) at 15 DAS. (Table 7).

Table 8: Effect of different treatments on the incidence of leaf folder in paddy

Sr. No.
Treatment Compositions Dosage/ha Leaf damage (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS 10 DAS 15 DAS
1 Cyclaniliprole 10% DC 40 400 6.19 (14.39) 8.73 (17.17) 11.41 (19.73) 56.64 58.90 53.30 - -
2 Cartap hydrochloride 50% SP 500 1000 7.54 (15.84) 10.90 (19.18) 13.89 (21.87) 47.13 48.67 43.14 - -
3 Cyclaniliprole 10% DC +Cartap hydrochloride 50% SP 40 +500 400+1000 2.19 (8.48) 2.90 (9.79) 3.97 (11.48) 84.67 86.34 83.77 1.09 1.14
4 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 25 + 250 500 3.41 (10.52) 4.73 (12.55) 6.67 (14.95) 76.12 77.71 72.71 0.98 0.99
5 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 30 + 300 600 1.83 (7.76) 2.30 (8.68) 3.07 (10.08) 87.15 89.17 87.45 1.13 1.19
6 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 35 + 350 700 1.56 (7.09) 2.00 (8.12) 2.87 (9.73) 89.09 90.58 88.27 1.14 1.20
7 Untreated Check - - 14.27 (22.13) 21.23 (27.41) 24.43 (29.59) - - - - -
CD at 5% 2.11 1.97 1.22 - - - - -
SE (m) 0.67 0.63 0.39 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray

Among all the doses of combination and individual treatments, Cyclaniliprole 5.0% + Cartap hydrochloride 50% @ 35 + 350 gm a.i./ha was observed with highest per cent reduction in incidence of leaf folder recorded with 88.27% control and found at par with Cyclaniliprole 5.0% + Cartap hydrochloride 50% @ 30 + 300 gm a.i./ha (87.45%) and Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha (83.77%) followed by Cyclaniliprole 5.0% + Cartap hydrochloride 50% @ 25 + 250 gm a.i./ha (72.71%) at 15th DAS.

All the doses of Cyclaniliprole 5.0% + Cartap hydrochloride 50%showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (53.30%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (43.14%) against leaf folder of rice at 15th day after spray. T3, T5 & T6 showed synergistic effect with the synergistic ratio 1.20, 1.19 & 1.14 (>1) at 15 DAS, respectively. (Table 8).

Table 9: Effect of different treatments on the incidence of stem borer in paddy

Sr. No.
Treatment Compositions Dosage/ha Dead Heart (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS 10 DAS 15 DAS
1 Cyclaniliprole 10% DC 40 400 1.89 (7.82) 2.04 (8.20) 2.02 (8.09) 65.49 67.96 64.62 - -
2 Cartap hydrochloride 50% SP 500 1000 2.51 (9.06) 2.77 (9.52) 2.66 (9.34) 54.23 56.44 53.33 - -
3 Cyclaniliprole 10%DC +Cartap hydrochloride 50% SP 40 +500 400+1000 0.57 (4.14) 0.68 (4.65) 0.72 (4.83) 89.65 89.37 87.31 1.03 1.04
4 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 15 + 200 500 1.38 (6.74) 1.52 (7.05) 1.58 (7.16) 74.74 76.07 72.34 0.88 0.86
5 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 18 + 240 600 1.22 (6.34) 1.30 (6.54) 1.41 (6.80) 77.66 79.58 75.32 0.92 0.90
6 Cyclaniliprole 3.0% +Cartap hydrochloride 40% 21 + 280 700 1.12 (6.07) 1.25 (6.40) 1.24 (6.36) 79.49 80.42 78.30 0.93 0.93
7 Untreated Check - - 5.48 (13.50) 6.37 (14.56) 5.70 (13.80) - - - - -
CD at 5% 1.67 1.67 1.52 - - - - -
SE (m) 0.53 0.53 0.49 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray
Dead hearts were pulled out after every observation from all the treatments including control

Results of Table 9:
• Among all the treatments the tank-mix combinations of Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha was observed with highest per cent reduction in incidence of stem borer recorded with 89.65%, 89.37%, 87.31% control at 5th, 10th and 15th day after spray, respectively followed by Cyclaniliprole 3.0% +Cartap hydrochloride 40% @ 21 + 280 gm a.i./ha, Cyclaniliprole 3.0% + Cartap hydrochloride 40% @ 18 + 240 gm a.i./ha and Cyclaniliprole 3.0% + Cartap hydrochloride 40% @ 15 + 200 gm a.i./ha.
• All the doses of pre-mix formulation of Cyclaniliprole 3.0% + Cartap hydrochloride 40% applied @21 + 280 gm a.i./ha, 18 + 240 gm a.i./ha and 15 + 200 gm a.i./ha observed with 78.30%, 75.32% and 72.34% reduction over untreated control at 15th DAS, respectively.
• All the doses of Cyclaniliprole 3.0% + Cartap hydrochloride 40% showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (64.62%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (53.33%) against stem borer of rice at 15th day after spray. Among the treatments only tank-mix combination of Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha showed synergistic effect with the synergistic ratio 1.046 (>1) at 15 DAS. (Table 9).

Table 10: Effect of different treatments on the incidence of stem borer in paddy

Sr. No.
Treatment Compositions Dosage/ha Dead Heart (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS 10 DAS 15 DAS
1 Cyclaniliprole 10% DC 40 400 2.17 (8.44) 1.80 (7.68) 2.28 (8.65) 63.28 66.25 62.97 - -
2 Cartap hydrochloride 50% SP 500 1000 2.69 (9.35) 2.37 (8.83) 2.95 (9.81) 54.35 55.63 52.16 - -
3 Cyclaniliprole 10%DC +Cartap hydrochloride 50% SP 40 +500 400+1000 0.67 (4.62) 0.57 (4.29) 0.77 (4.98) 88.64 89.31 87.57 1.05 1.06
4 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 20 + 225 500 1.32 (6.59) 1.10 (6.00) 1.46 (6.93) 77.63 79.38 76.32 0.93 0.92
5 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 24 + 270 600 0.52 (4.12) 0.37 (3.41) 0.55 (4.20) 91.13 93.13 91.14 1.09 1.10
6 Cyclaniliprole 4.0% +Cartap hydrochloride 45% 28 + 315 700 0.47 (3.88) 0.30 (3.10) 0.48 (3.93) 92.03 94.38 92.22 1.11 1.12
7 Untreated Check - - 5.90 (14.01) 5.33 (13.29) 6.17 (14.35) - - - - -
CD at 5% 1.87 1.53 1.71 - - - - -
SE (m) 0.60 0.49 0.54 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray
Dead hearts were pulled out after every observation from all the treatments including control

Results of Table 10:
• Among all the doses of combination and individual treatments, Cyclaniliprole 4.0% +Cartap hydrochloride 45% @ 28 + 315 gm a.i./ha was observed with highest per cent reduction in incidence of stem borer recorded with 92.22% control at 15th DAS and found at par with Cyclaniliprole 4.0% +Cartap hydrochloride 45% @ 24 + 270 gm a.i./ha (91.14%) and Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha (87.57%) followed by Cyclaniliprole 4.0% +Cartap hydrochloride 45% @ 20 + 225 gm a.i./ha (76.32%) at 15th DAS.
• All the doses of Cyclaniliprole 4.0% +Cartap hydrochloride 45% showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (62.97%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (52.16%) against stem borer of rice at 15th day after spray. Cyclaniliprole 4.0% +Cartap hydrochloride 45% @ 24 + 270 gm a.i./ha showed synergistic effect with the synergistic ratio1.108 (>1) at 15 DAS. (Table 10).

Table 11: Effect of different treatments on the incidence of stem borer in paddy

Sr. No.
Treatment Compositions Dosage/ha Dead Heart (%) Per cent reduction over control Colby’s synergistic ratio
Dosage/ha
a.i. (gm) Formulation (gm/ml) 5 DAS 10 DAS 15 DAS 5 DAS 10 DAS 15 DAS 10 DAS 15 DAS
1 Cyclaniliprole 10% DC 40 400 1.88 (7.83) 1.94 (7.99) 2.03 (8.19) 66.15 69.02 65.44 - -
2 Cartap hydrochloride 50% SP 500 1000 2.42 (8.92) 2.69 (9.40) 2.76 (9.56) 56.28 57.12 53.03 - -
3 Cyclaniliprole 10% DC +Cartap hydrochloride 50% SP 40 +500 400+1000 0.59 (4.34) 0.55 (4.20) 0.70 (4.78) 89.36 91.18 88.10 1.05 1.05
4 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 25 + 250 500 1.20 (6.27) 1.19 (6.24) 1.38 (6.73) 78.35 81.03 76.49 0.93 0.91
5 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 30 + 300 600 0.42 (3.67) 0.34 (3.31) 0.44 (3.73) 92.48 94.58 92.46 1.09 1.10
6 Cyclaniliprole 5.0% +Cartap hydrochloride 50% 35 + 350 700 0.31 (3.17) 0.30 (3.14) 0.39 (3.57) 94.41 95.16 93.37 1.09 1.11
7 Untreated Check - - 5.54 (13.52) 6.27 (14.41) 5.88 (13.89) - - - - -
CD at 5% 1.72 1.73 1.85 - - - - -
SE (m) 0.55 0.55 0.59 - - - - -
Figure in parenthesis represents angular transformed value; DAS: Days After Spray
Dead hearts were pulled out after every observation from all the treatments including control

• Among all the doses of combination and individual treatments, Cyclaniliprole 5.0% +Cartap hydrochloride 50% @ 35 + 350 gm a.i./ha was observed with highest per cent reduction in incidence of stem borer recorded with 93.37% control and found at par with Cyclaniliprole 5.0% +Cartap hydrochloride 50% @ 30 + 300 gm a.i./ha (92.46%) and Cyclaniliprole 10% DC @ 40 gm a.i./ha + Cartap hydrochloride 50% SP @ 500 gm a.i./ha (88.10%) followed by Cyclaniliprole 5.0% +Cartap hydrochloride 50% @ 25 + 250 gm a.i./ha (76.49%) at 15th DAS.
• All the doses of Cyclaniliprole 5.0% + Cartap hydrochloride 50% showed better efficacy compared to the individual applications of Cyclaniliprole 10% DC @ 40 gm a.i./ha (65.44%) and Cartap Hydrochloride 50% SP @ 500 gm a.i./ha (53.03%) against stem borer of rice at 15th day after spray. T3, T5 & T6 showed synergistic effect with the synergistic ratio 1.11, 1.10 & 1.05 (>1) at 15 DAS, respectively. (Table 11).

Phytotoxicity observations:
Various insecticidal compositions of the present invention provided good control of rice leaf folder and stem borer as compared to the reference products. Further, the use of these insecticidal compositions resulted in better crop condition i.e. fresh green leaves and didn’t produce any phytotoxic symptoms on the plants. Table 12-14 depicts the phytotoxic effects of the various insecticidal compositions of the present invention on paddy at 5th, 10th and 15th DAS.

Table 12: Phytotoxic effects of the insecticidal combination of “Cyclaniliprole 3.0% + Cartap hydrochloride 40%” on paddy
Treatments Phytotoxicity rating
Yellowing Wilting Necrosis Epinasty Hyponasty
Days After Spray
5 10 15 5 10 15 5 10 15 5 10 15 5 10 15
T1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Table 13: Phytotoxic effects of the insecticidal combination of “Cyclaniliprole 4.0% + Cartap hydrochloride 45%” on paddy
Treatments Phytotoxicity rating
Yellowing Wilting Necrosis Epinasty Hyponasty
Days After Spray
5 10 15 5 10 15 5 10 15 5 10 15 5 10 15
T1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Table 14: Phytotoxic effects of the insecticidal combination of “Cyclaniliprole 5.0% + Cartap hydrochloride 50%” on paddy
Treatments Phytotoxicity rating
Yellowing Wilting Necrosis Epinasty Hyponasty
Days After Spray
5 10 15 5 10 15 5 10 15 5 10 15 5 10 15
T1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
T7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Conclusion
After evaluation of three formulations i.e., Cyclaniliprole 3.0% + Cartap hydrochloride 40%, Cyclaniliprole 4.0% + Cartap hydrochloride 45% and Cyclaniliprole 5.0% + Cartap hydrochloride 50% at three different doses (500 ml/ ha, 600 ml/ha &700 ml/ha).
? Cyclaniliprole 4.0% + Cartap hydrochloride 45% @ 600 ml/ ha found to be effective against Leaf folder and Stem borer of Rice crop in comparison to Tank mix formulation (Cyclaniliprole 10% DC + Cartap hydrochloride 50% SP) as well as solo treatments (Cyclaniliprole 10% DC, Cartap hydrochloride 50% SP).
? Cyclaniliprole4.0% + Cartap hydrochloride 45% @ 600 ml/ ha was found at par with higher formulation, higher dose and higher active ingredient.
? Therefore, application of Cyclaniliprole 4.0% + Cartap hydrochloride 45% @ 600 ml/ ha (24 + 270 gm a.i./ ha) formulation can be recommended for effective control of Leaf folder and Stem borer in Rice crop.
ADVANTAGES OF THE PRESENT COMPOSITION
• The present disclosure provides a new and improved Synergistic composition that may overcome the limitations associated with the conventional insecticidal composition.
• The present disclosure provides a synergistic composition that exhibits broad spectrum insecticidal properties at lower dosage.
• The present disclosure provides a Synergistic composition that is safe to use.
• The present disclosure provides Synergistic composition that is cost-effective.
• The present disclosure provides Synergistic composition that is easy to prepare
, Claims:
1. An insecticidal composition, comprising:
(a) 0.1% to about 30.0% Cyclaniliprole, by weight of the composition;
(b) 0.1% to about 70.0% Cartap hydrochloride by weight of the composition; and
c) agriculturally accepted excipient(s).

2. The insecticidal composition as claimed in claim 1, wherein the agriculturally accepted excipient is selected from the group comprising emulsifier; dispersing agent; wetting agent; antifoaming agent; rheology modifier; Super-Wetting-spreading-penetrating agent; solvent; pH modifier; inert carrier; biocide; an antifreezing agent; pH Stabilizers ; stabilizing agent; and coloring agent wherein the composition comprises- about 0.1% to about 20.0% emulsifier; about 0.1% to about 20.0% dispersing agent; about 0.1% to about 15.0% wetting agent; about 0.01% to about 5.0% antifoaming agent; about 0.01% to about 20.0% rheology modifier; about 0% to about 95.0% solvent; about 0% to 35% co-solvent; about 0% to 90.0 % inert carrier; about 0% to 3 % biocide; 0.01 to 10% pH stabilizers; about 0% to 15 % antifreezing agent; about 0.1% to 5.0 % Super-Wetting-spreading-penetrating agent; 0% to 5 % stabilizing agent; 0% to 5 % pH modifier; and 0.01% to 5.0 % coloring agent.

3. The insecticidal composition as claimed in claim 3, wherein the emulsifier is selected from the group comprising ethoxylated propoxylated alcohols, alkylphenolethoxylates, alkoxylatedtristyrylphenols, calcium dodecylbenzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylated ricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof.

4. The insecticidal composition as claimed in claim 3, wherein the dispersing agent is selected from the group comprising polymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate, sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700, butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenol ethoxylates and polyalkylene oxide derivative of a synthetic alcohol, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof.

5. The insecticidal composition as claimed in claim 3, wherein the wetting agent is selected from the group comprising sodium alkyl naphthalene sulfonate, alpha olefin sulfonates, disodium laureth sulfosuccinate, diisodecyl sodium sulfosuccinate, alkyl sulfosuccinic monoesters, dioctyl sulfosuccinate sodium salt, sulfonic acids C14-16-alkane hydroxy and C14-16-alkene sodium salts, polyoxyethylene sorbitan monooleate, polyoxyethylene ether or mixtures thereof.

6. The insecticidal composition as claimed in claim 3, wherein the antifoaming agent is selected from the group comprising polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof.

7. The insecticidal composition as claimed in claim 3, wherein the rheology modifier is selected from the group comprising precipitated silica, fumed silica, modified fumed silica, bentonite, hydroxymethyl cellulose, carboxymethyl cellulose, xanthan gum, thickening silica, hydrated clay minerals, magnesium aluminium silicates, organic derivative of hectorite clay, hydrophobic fumed silica, polyvinylpyrrolidone (PVP) or mixture thereof.

8. The insecticidal composition as claimed in claim 3, wherein the solvent is selected Demineralized (DM) water, N-alkyl-pyrrolidone, oil medium selected from the group comprising, esterified fatty acids selected from methyl and/or ethyl ester of vegetable oil such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester or mixtures thereof.

9. The insecticidal composition as claimed in claim 3, wherein inert carrier is selected from the group comprising kaolin, china clay, dextrin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof.

10. The insecticidal composition as claimed in claim 3, wherein the anti freezing agent is selected from the group comprising ethylene glycol, 1,2-propanediol, propane-1,2,3-triol, urea or mixtures thereof.

11. The insecticidal composition as claimed in claim 3, wherein the Super-Wetting-spreading-penetrating agent is blend of methylated seed oil-organic silicone compound may be selected from methylated seed oil-polyalkyleneoxide modified trisiloxane, methylated seed oil-polyalkyleneoxide modified polydimethylsiloxane, methylated seed oil-20 trisiloxane ethoxylate, Polyalkyleneoxide modified heptamethyltrisiloxane methylated seed oil-polyoxyethylene methyl polysiloxane, methylated seed oil-polyether polymethyl siloxane copolymer, methylated seed oil-polyether modified polysiloxane. or mixtures thereof.

12. The insecticidal composition as claimed in claim 3, wherein the stabilizing agent is selected from the group comprising epoxidized soyabean oil, butylated hydroxy toluene, ethylenediaminetetraacetic acid, sodium benzoate, etc. or mixtures thereof.

13. The insecticidal composition as claimed in claim 3, wherein the pH modifier is selected from the group comprising sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, tartaric acid, hydrochloric acid or mixtures thereof.

14. The insecticidal composition as claimed in claim 3, wherein the pH Stabilizers selected from the group comprising of sodium pyrophosphate, sodium acetate, sodium oxalate, sodium carbonate, sodium bicarbonate, trisodium phosphate, citric acid, trisodium citrate, monoethanol amine, triethanol amine, triethylamine, dibasic esters selected from dimethyl succinate, dimethyl glutarate, dimethyl adipate, ortho phosphoric acid, oxalic acid, citric acid, hydrochloric acid.

15. The insecticidal composition as claimed in claim 3, wherein the coloring agent is selected from from selected from dye, pigment or mixtures thereof such as Triaryl methane acid blue, 3-hydroxy-N-(2-methylphenyl)-4-[(E)-(2,4,5-trichlorophenyl)diazinyl]-2-naphthamide and Acid Brilliant Green.
16. The process of preparation of an insecticidal composition as claimed in claim 1, the process comprising: preparation of the said composition with agriculturally acceptable excipients.