Description:Title of the Invention
“A Diagnostic Test Kit for Triglycerides”
Field of the Invention
The present disclosure relates generally to field of medical devices. More specifically the present invention relates to diagnostic test kit works on photometric method to determine Triglycerides from blood serum / plasma in vitro for laboratories and medical professionals.
Background of Invention
A triglycerides test is a blood test that measures the amount of a fat in your blood called triglycerides. High triglycerides may increase your risk for a heart attack or stroke. A triglycerides test can help you decide if you need to take action to lower your risk. Your body uses triglycerides for energy. If you eat more calories than you need, your body turns the extra calories into triglycerides and stores them in your fat cells to use later. When your body needs energy, your cells release triglycerides into your bloodstream to provide fuel for your muscles to work. If you eat more calories than you burn off, especially calories from carbohydrates, including sugary foods, and fats, you may have high triglyceride levels in your blood. A high blood triglyceride level usually doesn't cause any symptoms, but over time, it may affect your arteries and increase your risk of heart disease. Extremely high levels of triglycerides also increase the risk of acute pancreatitis in adults and children. A triglycerides test is used to understand your risk for heart disease, stroke, and other conditions that involve your arteries, such as peripheral arterial disease. The test is also used to help monitor heart conditions and treatments to lower the risk of heart disease. A triglycerides test is usually done as part of a group of tests called a lipid profile. Lipid is another word for fat. A lipid profile measures the level of fats in your blood, including triglycerides and cholesterol. If you have high levels of both LDL (bad) cholesterol and triglycerides, you may have a higher risk for a heart attack or stroke.

Therefore, there is a need for a device that overcomes at least the above mentioned problems. There is a need for a rapid, precise, and cost-effective diagnostic kit and method for the measurement of Triglycerides.
The present disclosure relates generally to field of medical devices. More specifically the present invention relates to diagnostic test kit works on photometric method to estimate Triglycerides from serum / plasma, in vitro for laboratories and medical professionals.
Object of the Invention
The primary object of the invention is to develop a diagnostic test kit to estimate Triglycerides from blood serum / plasma useful effectively.
The secondary objective of the invention is that the diagnostic test kit for Triglycerides utilizes photometric method herein the kit configured with suitable reagents like Pipes, Lipoprotein Lipase, Glycerol Kinase, Peroxidase and Triglycerides as standard, taken in effective concentration of blood serum /plasma.
Another objective of the invention is that the prepared blood serum/plasma samples with reagents estimated for absorbance in photometer at desired wavelength and room temperature.
Further objective of the invention is that Triglycerides is determined through calculation by the absorbance of sample and standard.
Summary of the Invention

The present disclosure relates a diagnostic kit specifically to determine Triglycerides concentration in the blood serum/plasma wherein the desired size kit is developed, essentially consist of reagents for reaction with blood serum/plasma samples, detected on photometer at desired wavelength to measure the absorbance and finally calculated to determine Triglycerides concentration.
A diagnostic test kit for Triglycerides comprising a test kit characterized in that 50 ml and 100 ml size kit of one compartment cell reagents preferably consist of Pipes, Lipoprotein Lipase, Glycerol Kinase, Peroxidase with Triglycerides as standard estimate Triglycerides in vitro from blood serum / plasma sample effectively, wherein reagent cell composed of 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase, 500 u/l of Peroxidase taken with blood serum/ plasma sample from 0.010 ml to 0.015 ml into two clean test tube for test sample and blank sample and Triglycerides 200 mg/dl sample as standard in third test tube; pipette out the reagent from the kit separately; transferring said reagent into the test tubes respectively at room temperature (37oC) and , uniformly mixed with test sample, blank sample and standard; incubating the reaction mixture for 10 minutes; determining the absorbance of said test sample blank sample and standard Triglycerides sample on photometer at a wavelength of 510 nm for 30-90 seconds; and finally calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.

One of the preferred embodiments of the present invention is the unique design of the kit prefilled with the preferred reagent like Phosphate Pipes, Lipoprotein Lipase, Glycerol Kinase and Peroxidase reacts with serum/ plasma Triglycerides evaluated through the absorbance on photometer.

One of the preferred embodiments of the present invention is that to determine Triglycerides from the varied concentration more preferably making the test sample, blank sample and Triglycerides sample as standard to infer the clinical findings effectively.

One of the preferred embodiments of Triglycerides is estimated in vitro through all in one reagent kit.

Brief Description of Drawings

The following thorough explanation of the various aspects of the invention, taken in conjunction with the corresponding drawing that represents various aspects and other features of the disclosure invention.

Figure 1: Flow chart of method of estimation of Triglycerides

Detailed Description of the Invention

The following description is of exemplary embodiments only and is not intended to limit the scope, applicability or configuration of the invention in any way. Rather, the following description provides a convenient illustration through explanation and figures for implementing exemplary embodiments of the invention. Various changes to the described embodiments may be made in the function and arrangement of the ingredients described without departing from the scope of the invention.
The use of “including”, “comprising” or “having” variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. The terms “a” and “an” herein do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced items. Further, the use of terms “first”, “second”, and “third”, and the like, herein do not denote any order, quantity, or importance, but rather are used to distinguish one element from another.
The terms used in the invention
mmol: milimole
mmol/l: millimole per liter
A triglycerides test is a blood test that measures the amount of a fat in your blood called triglycerides. High triglycerides may increase your risk for a heart attack or stroke. A triglycerides test can help you decide if you need to take action to lower your risk. Your body uses triglycerides for energy. If you eat more calories than you need, your body turns the extra calories into triglycerides and stores them in your fat cells to use later. When your body needs energy, your cells release triglycerides into your bloodstream to provide fuel for your muscles to work. If you eat more calories than you burn off, especially calories from carbohydrates, including sugary foods, and fats, you may have high triglyceride levels in your blood. A high blood triglyceride level usually doesn't cause any symptoms, but over time, it may affect your arteries and increase your risk of heart disease.
A diagnostic test kit for Triglycerides comprising a test kit characterized in that 50 ml and 100 ml size kit of one compartment cell reagents preferably consist of Pipes, Lipoprotein Lipase, Glycerol Kinase, Peroxidase with Triglycerides as standard estimate Triglycerides in vitro from blood serum / plasma sample effectively, wherein reagent cell composed of 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase, 500 u/l of Peroxidase taken with blood serum/ plasma sample from 0.010 ml to 0.015 ml into two clean test tube for test sample and blank sample and Triglycerides 200 mg/dl sample as standard in third test tube; pipette out the reagent from the kit separately; transferring said reagent into the test tubes respectively at room temperature (37oC) and , uniformly mixed with test sample, blank sample and standard; incubating the reaction mixture for 10 minutes; determining the absorbance of said test sample blank sample and standard Triglycerides sample on photometer at a wavelength of 510 nm for 30-90 seconds; and finally calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.
The present disclosure relates a diagnostic kit specifically to determine Triglycerides concentration in the blood serum/plasma wherein the desired size kit is developed, essentially consists of reagents for reaction with blood serum/plasma samples, detected on photometer at desired wavelength to measure the absorbance and finally calculated the Triglycerides concentration.
Preferred embodiments of a diagnostic kit for Triglycerides
i) Diagnostic Kit: The Triglycerides kit comprises of 50 ml and 100 ml size wherein reagent cell prefilled for the determination of Triglycerides, herein the key reagent like Pipes, Lipoprotein Lipase, Glycerol Kinase and Peroxidase reacts with serum/ plasma Triglycerides used in different concentration, estimates serum / plasma Triglycerides through the absorbance using photometer of desired wavelength.
ii) Reagents: Blood serum /plasma, Pipes, Lipoprotein Lipase, Glycerol Kinase, and Peroxidase
iii) Apparatus / Instrument: Test tubes, Photometer, Incubator etc.
Table no. 01: Preferred embodiments and parameters of the kit with concentrations

Particulars Name of ingredient with concentration
Serum/ plasma sample 0.010 ml to 0.015 ml
Reagent 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase
Standard (Triglycerides) 200 mg/dl
Shelf Life 24 months
Kit Size 50 ml and 100 ml
Wavelength 510 nm
Temperature 37oC
Time of Estimation 30 to 90 seconds

Table no. 02: Method to operate Triglycerides

Particulars Blank Standard Sample
Reagent 1.0 ml 1.0 ml 1.0 ml
Standard -- 0.010 ml --
Serum/plasma test sample -- -- 0.010 to 0.015 ml

General Method to operate the device

Taking the 0.010 to 0.015 ml blood serum/ plasma sample as test sample, reagent as blank sample and Triglycerides as standard separately in clean test tubes;
pipette out the reagent 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase from the kit separately; transferring said reagent into the test tubes respectively at room temperature (37oC); incubating the reaction mixture for 10 minutes; determining the absorbance of said test sample, blank sample and 200 mg/dl Triglycerides sample as standard, on photometer at a wavelength of 510 nm for 30-90 seconds; and finally calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.

The absorbance is calculated using below formula:
Concentration = Absorption of sample / Absorption of standard x 200 mg/dl
(Table No. 1, 2 and Figure No. 1)
Example 1
Taking the 0.010 ml blood serum/ plasma sample as test sample, reagent as blank sample and Triglycerides as standard separately in clean test tubes;
pipette out the reagent 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase from the kit separately; transferring said reagent into the test tubes respectively at room temperature (37oC); incubating the reaction mixture for 10 minutes; determining the absorbance of said test sample, blank sample and 200 mg/dl Triglycerides sample as standard, on photometer at a wavelength of 510 nm for 30-90 seconds; and finally calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.

The absorbance is calculated using below formula:
Concentration = Absorption of sample / Absorption of standard x 200 mg/dl

Example 2
Taking the 0.015 ml blood serum/ plasma sample as test sample, reagent as blank sample and Triglycerides as standard separately in clean test tubes;
pipette out the reagent 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase from the kit separately; transferring said reagent into the test tubes respectively at room temperature (37oC); incubating the reaction mixture for 10 minutes; determining the absorbance of said test sample, blank sample and 200 mg/dl Triglycerides sample as standard, on photometer at a wavelength of 510 nm for 30-90 seconds; and finally calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.

The absorbance is calculated using below formula:
Concentration = Absorption of sample / Absorption of standard x 200 mg/dl
Scope of the Invention

A diagnostic test kit for Triglycerides principally design to develop a simple method to analyse the Triglycerides level using photometric method having size of 50 ml and 100 ml size kit of reagent preferably consist of effective concentration of 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase, taken with blood serum/ plasma sample from 0.010 ml to 0.015 ml as test sample and blank sample and Triglycerides 200 mg/dl sample as standard respectively determines Triglycerides in vitro from blood serum / plasma sample effectively, could be potential diagnostic and economical method, compare to expensive conventional methods in future.

It is to be understood that the present invention is not limited to the embodiments described above, it should be clear that various modifications and alterations can be made along with various features of one embodiment included in other embodiments, within the scope of the present invention.
, Claims:We claim,
1- A diagnostic test kit for Triglycerides comprising a test kit characterized in that 50 ml and 100 ml size kit of reagent preferably consist of effective concentration of 100 mmol/l of Pipes, 2 ku/l of Lipoprotein Lipase, 300 u/l of Glycerol Kinase and 500 u/l of Peroxidase estimates Triglycerides in vitro from blood serum / plasma sample taken from 0.010 to 0.015 ml as test sample and 200 mg/dl of Triglycerides as standard, using photometer effectively.

2- The diagnostic test kit for Triglycerides as claimed in claim 1, operates through in vitro diagnostic method to determine Triglycerides comprises the following steps:
taking the 0.010 to 0.015 ml blood serum/ plasma sample as test sample, reagent as blank sample and Triglycerides as standard separately in clean test tubes;
pipette out the reagent Pipes, Lipoprotein Lipase, Glycerol Kinase and Peroxidase from the kit separately;
transferring said reagent into the test tubes respectively at room temperature (37oC);
incubating the reaction mixture for 10 minutes;
determining the absorbance of said test sample, blank sample and standard Triglycerides sample on photometer at a wavelength of 510 nm for 30-90 seconds; and
calculating the absorbance to estimate Triglycerides from the test serum / plasma sample effectively.