DESC:TECHNICAL FIELD
[1] The present disclosure relates generally to the field of aquaculture technologies utilized in live fish transportation and, more specifically, to a chemical formulation system and a method for long-distance live fish transportation.
BACKGROUND
[2] Live fish transportation is a process, which is required in the aquaculture industry for ensuring the safe and viable transfer of the live fish from hatcheries to grow-out farms, markets, or stocking sites, while maintaining the health, quality, and survival of the fish. Moreover, in the live fish transportation the fish survival and minimal stress on the viability of the live fish is required for preserving the live fish health, maintaining economic value, and avoiding significant post-transport mortality. During transportation, the live fish are confined in containers and exposed to dynamic environmental conditions, such as fluctuating oxygen levels, accumulation of toxic substances like ammonia, changes in pH, temperature variations and the like. Furthermore, the dynamic environmental conditions collectively affect fish welfare and eventually lead to high mortality rates, especially during long-distance transport. In order to reduce the effects caused by the dynamic environmental conditions, the live fish transportation method and systems must regulate water quality parameters and provide a controlled environment that supports the live fish physiology.
[3] Conventional live fish transport methods and systems often rely on periodic water exchanges and manual dosing of water treatments to maintain acceptable water quality. Furthermore, the conventional live fish transport methods and systems utilize excessive amounts of water, replacing up to 50% of the transport container volume every 30 kilometres and still lack to adequately control parameters, such as dissolved oxygen, ammonia levels, and the like. Moreover, such methods and systems do not allow real-time water quality adjustments or stress reduction through biologically compatible formulations. Therefore, the conventional live fish transport methods and systems increase operational complexity, waste resources, and fail to ensure consistently high survival rates across different live fish species and transport conditions. As a result, there exists a technical problem of how to maintain the water quality parameters, such as the dissolved oxygen, the pH, and the ammonia concentration consistently throughout long-distance live fish transport.
[4] Therefore, in light of the foregoing discussion, there exists a need to overcome the aforementioned drawbacks associated with the conventional live fish transport methods and the systems.
SUMMARY
[5] The present disclosure provides a chemical formulation system and method for long-distance live fish transportation. The present disclosure provides a solution to the technical problem of how to maintain the water quality parameters, such as a dissolved oxygen, a pH, and an ammonia concentration consistently throughout long-distance live fish transport. An aim of the present disclosure is to provide a solution that overcomes at least partially the problems encountered in the prior art and provide the chemical formulation and method for long-distance live fish transportation while minimizing the live fish stress and avoiding frequent water exchanges, without adding complexity or increasing resource consumption.
[6] One or more objectives of the present disclosure is achieved by the solutions provided in the enclosed independent claims. Advantageous implementations of the present disclosure are further defined in the dependent claims.
[7] In one aspect, the present disclosure provides a chemical formulation for long-distance live fish transport, the chemical formulation that includes an oxygen-releasing compounds, an ammonia control agent, a live fish stress reduction compounds and an ionic balance regulators. Moreover, the chemical formulation is configured to maintain water quality during the live fish transport without requiring water exchange during transport.
[8] Advantageously, the chemical formulation is configured to maintain the water quality during long-distance live fish transport through a synergistic blend of the oxygen-releasing compounds, the ammonia control agents, the fish stress-reduction compounds, and the ionic balance regulators. Moreover, the oxygen-releasing compounds ensure a sustained supply of the dissolved oxygen throughout the transportation, while the ammonia control agents adsorb or neutralize toxic nitrogenous wastes, maintaining water safety. Furthermore, the inclusion of the stress-reducing agents, such as herbal sedatives and antioxidants allows for lowering fish cortisol levels, thereby minimizing physiological distress. Furthermore, the ionic balance regulators, such as calcium, potassium, and magnesium chlorides maintain osmotic equilibrium, enhancing the live fish metabolism under confined transport conditions. Additionally, the chemical formulation eliminates the requirement for frequent water exchanges by stabilizing key parameters within safe thresholds, thereby reducing operational complexity, water usage, and environmental impact, while significantly enhancing the live fish survival rates over distances.
[9] In another aspect, the system for long-distance transport of live fish includes a transport tank, water quality sensors configured to monitor dissolved oxygen, pH, temperature, and ammonia levels, a controller configured to process real-time water quality data, an automated dosing module operatively connected to the controller, wherein the controller is configured to activate the dosing mechanism to supply the chemical formulation based on the monitored water quality data.
[10] Advantageously, the system for the long-distance transport of the live fish is configured to dynamically monitor and maintain the water quality through the integration of real-time sensing and automated dosing. The water quality sensors continuously track parameters, such as the dissolved oxygen, the pH, the temperature, and the ammonia levels within the transport tank. The controller processes the real-time data and initiates the automated dosing module to supply amounts of the chemical formulation when any parameter deviates from a predefined threshold. The closed-loop control enables responsive adjustments to water chemistry during transit, ensuring a stable aquatic environment. Furthermore, the automated dosing module minimizes human intervention, reduces the risk of manual dosing errors, and ensures consistency across multiple transport operations. By maintaining the water quality within limits, the system enhances the live fish welfare, reduces mortality, and extends transport capabilities with minimal or no water exchange, thereby enhancing operational sustainability and efficiency in aquaculture logistics.
[11] In another aspect, a method for long-distance transport of live fish, the method includes, harvesting fish from a culture environment, loading the harvested fish into a transport tank, monitoring water quality parameters using water quality sensors connected to a controller, adding a chemical formulation to the transport tank based on the monitored water quality parameters and transporting the fish without requiring water exchange during transport.
[12] The method achieves all the advantages and technical effects of the system of the present disclosure.
[13] It is to be appreciated that all the aforementioned implementation forms can be combined. All steps that are performed by the various entities described in the present application, as well as the functionalities described to be performed by the various entities, are intended to mean that the respective entity is adapted to or configured to perform the respective steps and functionalities. It will be appreciated that features of the present disclosure are susceptible to being combined in various combinations without departing from the scope of the present disclosure as defined by the appended claims.
[14] Additional aspects, advantages, features, and objects of the present disclosure would be made apparent from the drawings and the detailed description of the illustrative implementations construed in conjunction with the appended claims that follow.
BRIEF DESCRIPTION OF THE DRAWINGS
[15] The summary above, as well as the following detailed description of illustrative embodiments, is better understood when read in conjunction with the appended drawings. For the purpose of illustrating the present disclosure, exemplary constructions of the disclosure are shown in the drawings. However, the present disclosure is not limited to specific methods and instrumentalities disclosed herein. Moreover, those skilled in the art will understand that the drawings are not to scale. Wherever possible, like elements have been indicated by identical numbers.
[16] Embodiments of the present disclosure will now be described, by way of example only, with reference to the following diagrams wherein:
FIG. 1 is a block diagram of a system for long-distance live fish transport, in accordance with an embodiment of the present disclosure;
FIG. 2 is a diagram illustrating an implementation scenario of the system for the long distance live fish transportation with chemical formulation, in accordance with an embodiment of the present disclosure;
FIG.3 is a flowchart of a method for the long-distance transport of the live fish, in accordance with an embodiment of the present disclosure;
FIG. 4 is a graphical representation illustrating the water quality parameter maintenance during the long-distance live fish transport, in accordance with an embodiment of the present disclosure;
FIG. 5 is a graphical representation illustrating the chemical formulation component effectiveness during the live fish transportation, in accordance with an embodiment of the present disclosure;
FIG. 6 is a graphical representation illustrating the survival rate performance at different transport distances by utilizing the chemical formulation system, in accordance with an embodiment of the present disclosure;
FIG. 7 is a graphical representation illustrating the water usage efficiency (cumulative percentage over distance) during the live fish transportation, in accordance with an embodiment of the present disclosure; and
FIG. 8 is a graphical representation illustrating the water parameter stability during 24-hour transport, in accordance with an embodiment of the present disclosure.
[17] In the accompanying drawings, an underlined number is employed to represent an item over which the underlined number is positioned or an item to which the underlined number is adjacent. A non-underlined number relates to an item identified by a line linking the non-underlined number to the item. When a number is non-underlined and accompanied by an associated arrow, the non-underlined number is used to identify a general item at which the arrow is pointing.
DETAILED DESCRIPTION OF EMBODIMENTS
[18] The following detailed description illustrates embodiments of the present disclosure and ways in which they can be implemented. Although some modes of carrying out the present disclosure have been disclosed, those skilled in the art would recognize that other embodiments for carrying out or practicing the present disclosure are also possible.
[19] FIG. 1 is a block diagram of a system for long-distance live fish transport, in accordance with an embodiment of the present disclosure. With reference to FIG. 1, there is shown the system 100 for long-distance live fish transport. The system 100 includes a transport tank 102, a controller 104, water quality sensors 106, an IoT device 108, an automatic dosing module 110, a testing apparatus 112, and a temperature controlling unit 114.
[20] The system 100 is configured to transport the live fish over long distances with maximum survival rate while maintaining water quality parameters without requiring water exchange during transportation of the live fish. Moreover, the system 100 is configured to integrate multiple components, such as the transport tank 102, the controller 104, the water quality sensors 106, the IoT devices 108, the automatic dosing module 110, and the temperature controlling unit 114 to form a sustainable transportation ecosystem. Furthermore, the system 100 is configured to enable commercial-scale fish distribution, reduce operational costs through zero water exchange requirements, and provide scalable deployment for aquaculture facilities, hatcheries, and fish farmers engaged in long-distance live fish commerce. Additionally, the system 100 is configured to eliminate the requirement for substantial groundwater usage and minimizes environmental impact while ensuring fish health and survival throughout the journey.
[21] The transport tank 102 refers to a component of the system 100 which is configured to hold and contain the live fish, such as in a container during long-distance transportation while maintaining the aquatic conditions without requiring water exchange. Moreover, the transport tank 102 is configured to accommodate harvested fish and provide adequate space for fish movement while supporting the integration of oxygenation systems, water quality monitoring equipment, and chemical dosing mechanisms. Additionally, the transport tank 102 is configured to maintain structural integrity during transportation and facilitates easy loading and unloading of the live fish while minimizing the handling stress during transportation of the live fish.
[001] The controller 104 refers to a component of the system 100 which is configured to manage central processing operations, coordinate real-time decisions, monitor the different parameters of the system 100, and execute automated control commands for maintaining the water conditions. Moreover, the controller 104 is configured to process the data from multiple sensors, analyse the water quality parameter against predefined thresholds, and activate responses through the automated dosing module 110. Additionally, the controller 104 is configured to maintain the various components of the system 100, ensure parameter monitoring accuracy, implement automated control protocols, and execute command sequences for the live fish welfare throughout transportation. The controller 104 may refer to one or more individual processors, processing devices, and various elements associated with a processing device that may be shared by other processing devices. Additionally, one or more individual processors, processing devices, and elements are arranged in various architectures for responding to and processing the instructions that drive the system 100. In some implementations, the controller 104 may be an independent unit and may be located outside the motherboard of the system 100. Examples of the controller 104 may include, but are not limited to, a hardware processor, a digital signal processor (DSP), a microprocessor, a microcontroller, a complex instruction set computing (CISC) processor, an application-specific integrated circuit (ASIC) processor, a reduced instruction set (RISC) processor, a very long instruction word (VLIW) processor, a state machine, a data processing unit, a graphics processing unit (GPU), and other processors or control circuitry or a processor forwarding system.
[22] The water quality sensors 106 refers to a component of the system 100, which is configured to continuously monitor and measure the dissolved oxygen levels, pH balance, temperature, and ammonia concentration by utilizing DO sensor, pH sensor, and NH3 sensor technologies. Moreover, the water quality sensors 106 is configured to provide real-time data transmission to the controller 104, enabling immediate response to parameter fluctuations that could affect the live fish health. Additionally, the water quality sensors 106 is configured to operate continuously throughout the transportation process, maintain measurement accuracy under varying conditions.
[23] The IoT device 108 refers to a component of the system 100 which is configured to provide internet connectivity, transmit real-time data, enable cloud connectivity, and facilitate remote monitoring of the transportation process. Moreover, the IoT device 108 is configured to enable data transmission of the water quality parameters, and operational metrics to remote monitoring stations. Additionally, the IoT device 108 is configured to support cloud-based data storage, allows for remote system diagnostics, enables real-time alerts and notifications, and facilitates tracking of transportation progress and the live fish welfare status throughout the transportation.
[24] The automatic dosing module 110 refers to a component of the system 100 which is configured to dispense a chemical formulation that contains different chemical compounds, based on real-time water quality readings and automated dosing requirements. Moreover, the automatic dosing module 110 includes a dosing mechanisms for an oxygen-releasing compounds, an ammonia control agent, a live fish stress reduction compounds, and an ionic balance regulator. Furthermore, the automatic dosing module 110 is configured to ensures timely addition of the chemical formulation to the water based on the predefined water quality thresholds, and act as per emergency dosing protocols when the dissolved oxygen within the transport tank 102 falls below 4 mg/L or ammonia exceeds 0.5 ppm.
[25] The testing apparatus 112 refers to a component of the system 100 which is configured to conduct multi-parameter water quality analysis, perform real-time testing, and ensure continuous monitoring of the water conditions throughout transportation. Moreover, the testing apparatus 112 is configured to support comprehensive water quality assessment, such as the dissolved oxygen measurement, the pH level analysis, the ammonia concentration testing, and the temperature monitoring. The testing apparatus 112 is configured to provide backup verification of sensor readings, enables quality control validation, supports calibration procedures, and ensures measurement reliability for the water parameters affecting the live fish survival.
[26] The temperature controlling unit 114 refers to a component of the system 100 which is configured to maintain stable water temperature through heating and cooling to minimize the live fish stress during transportation. Furthermore, the temperature controlling unit 114 is configured to monitor ambient temperature variations, adjust water temperature to the required ranges for the live fish species being transported, prevent thermal shock during loading and unloading procedures, and maintain consistent thermal conditions throughout the transportation. The temperature controlling unit 114 incorporates both heating and cooling capabilities to accommodate varying external environmental conditions and seasonal temperature fluctuations.
[27] There is provided the system 100 for long-distance transport of live fish includes the transport tank 102, the water quality sensors 106 that are configured to monitor the dissolved oxygen, the pH, the temperature, and the ammonia levels. Furthermore, the system 100 is configured to provide continuous monitoring of the water parameters, such as the dissolved oxygen, the pH, the temperature, the ammonia levels and the like that directly affect the live fish survival and health during long-distance transport of the live fish. The transport tank 102 is configured to provide a controlled aquatic environment with 2500-liter capacity configured to accommodate the live fish while providing the required conditions throughout the transportation. Moreover, the water quality sensors 106 is configured to function as the primary detection system that continuously measures the dissolved oxygen levels to ensure adequate respiration, monitors the pH levels to maintain acid-base balance, tracks the temperature variations to prevent thermal stress, and detects the ammonia concentrations to prevent toxic accumulation from the live fish waste products. Furthermore, the configuration of the water quality sensors 106 enables water quality assessment through the multi water parameters monitoring that provides real-time data on all factors affecting the live fish health and survival. Additionally, the monitoring of the water parameters operates continuously throughout the live fish transportation to detect fluctuations before fall below predefined threshold, enabling proactive intervention and maintenance of optimal aquatic conditions. The water quality sensors 106 ensures accurate measurement and data collection that supports automated decision-making processes for water quality management. As a result, the system 100 provides comprehensive monitoring of the water parameters that enable early detection of water quality issues, ensure continuous assessment of the dissolved oxygen, the pH, the temperature, and the ammonia levels affecting the live fish health, enhance proactive water quality management, prevent fluctuations that affect the fish survival.
[28] Furthermore, the system 100 includes the controller 104, which is configured to process real-time water quality data and the automatic dosing module 110 operatively connected to the controller 104, wherein the controller 104 is configured to activate the automatic dosing module 110 to supply a chemical formulation based on the monitored water quality data. In an implementation, the system 100 provides an automated control and chemical addition of the chemical formulation that enable the water quality management based on the actual aquatic conditions and the live fish welfare requirements throughout the long distance transportation of the live fish. The controller 104 is configured to act as the central processing unit that continuously receive and analyse the incoming sensor data from the water quality sensors 106, compare measured water quality parameters against the predetermined safety thresholds, and execute decision-making algorithms to determine the chemical dosing requirement for maintaining balanced aquatic conditions. Furthermore, the automatic dosing module 110 is configured to operate under the controller 104 to provide the predefined quantities of the different components of the chemical formulation, such as oxygen-releasing compounds when the dissolved oxygen levels drop below 4 mg/L, ammonia control agents when ammonia concentrations exceed 0.5 ppm, stress reduction compounds during periods of handling stress, ionic balance regulators when electrolyte levels require adjustment. Moreover, the connection between the controller 104 and the automatic dosing module 110 enables immediate automated response to the water parameters fluctuations without requiring human intervention, ensuring consistent chemical addition that maintains water quality within ranges throughout the live fish transportation. As a result, the system 100 provides the water quality management that eliminates the manual monitoring and dosing requirements, ensures immediate response to the water parameter changes, maintains the chemical formulation concentrations for the live fish health and survival.
[29] Advantageously, the system 100 for the long-distance transport of the live fish is configured to dynamically monitor and maintain the water quality through the integration of real-time sensing and automated dosing. The water quality sensors 106 are configured to continuously track parameters, such as the dissolved oxygen, the pH, the temperature, and the ammonia levels within the transport tank. The controller 104 is configured to process the real-time data and initiates the automated dosing module to supply amounts of the chemical formulation when any parameter deviates from a predefined threshold. The closed-loop control enables responsive adjustments to water chemistry during transit, ensuring a stable aquatic environment. Furthermore, the automated dosing module 110 is configured to minimize human intervention, reduces the risk of manual dosing errors, and ensures consistency across multiple transport operations. By maintaining the water quality within limits, the system enhances the live fish welfare, reduces mortality, and extends transport capabilities with minimal or no water exchange, thereby enhancing operational sustainability and efficiency in aquaculture logistics.
[30] FIG. 2 is a diagram illustrating an implementation scenario of the system for the long distance live fish transportation with chemical formulation, in accordance with an embodiment of the present disclosure. FIG. 2 is described in conjunction with the elements of the FIG. 1. With reference to FIG. 2, there is shown a diagram 200 that depicts the process of the live fish transportation. The diagram 200 includes the transport tank 102 having a plurality of fishes 202. Furthermore, the transport tank 102 includes the water quality sensors 106. Moreover, the diagram 200 includes the chemical formulation 204, which is being sprinkled inside the transport tank.
[31] The plurality of fishes 202 refers to aquatic organisms being acclimatized within the transport tank 102. The plurality of fishes 202 incorporates a wide variety of fishes, which may include but not limited to tilapia (or Oreochromis niloticus), common carp (or Cyprinus carpio), African catfish (or Clarias gariepinus), rohu (or Labeo rohita), catla (or Catla catla), mrigal (or Cirrhinus mrigala), grass carp (or Ctenopharyngodon idella), silver carp (or Hypophthalmichthys molitrix), bighead carp (or Hypophthalmichthys nobilis), striped bass (or Morone saxatilis), channel catfish (or Ictalurus punctatus), rainbow trout (or Oncorhynchus mykiss), salmon species, pangasius (or Pangasianodon hypophthalmus), snakehead fish (or Channa species), freshwater prawns, or any commercially cultured aquatic species suitable for live transport and requiring stress reduction during post-harvest handling and acclimatization procedures.
[32] There is provided, the chemical formulation 204 utilized for long distance live fish transport, wherein the chemical formulation 204 includes the oxygen-releasing compounds, ammonia control agents, live fish stress reduction compounds and the ionic balance regulators. The chemical formulation 204 utilized for long distance live fish transport is configured to maintain the water quality during extended transportation of the live fish. Moreover, the inclusion of the oxygen-releasing compounds in the chemical formulation 204 ensure the continuous dissolved oxygen availability to maintain the live fish respiration and prevent hypoxic stress, while the ammonia control agents within the chemical formulation 204 is configured to neutralize the toxic ammonia buildup that accumulates from the live fish waste and metabolic processes during confinement. Furthermore, the inclusion of the live fish stress reduction compounds in the chemical formulation 204 minimize cortisol production and physiological stress responses which is activated by environmental changes, such as the handling procedures, confinement conditions, water movement, temperature fluctuations, sudden changes in lighting, noise levels, and the like during transportation of the live fish. Furthermore, the ionic balance regulators within the chemical formulation 204 is configured to maintain required mineral concentrations and osmotic equilibrium, which is required for the fish cellular function. As a result, the chemical formulation 204 utilized for long distance live fish water is configured to form a stable aquatic environment that replicates natural pond conditions, thereby enabling long-distance fish transportation without the requirement for frequent water changes while significantly enhancing the live fish survival rates and reducing transportation-related mortality.
[33] Furthermore, the chemical formulation 204 is configured to maintain water quality during the long fish transport without requiring water exchange during transport. In an implementation, the chemical formulation 204 is configured to ensure the continuous water quality and stability through the synergistic effect of the different chemical of the chemical formulation 204 that actively regulates and maintains optimal aquatic conditions throughout the fish transportation. Furthermore, the chemical formulation 204 is configured to provide the sustained dissolved oxygen levels through the oxygen-releasing compounds that deliver controlled oxygen release over extended periods, while the ammonia control agents in the chemical formulation 204 is configured to continuously neutralize toxic ammonia buildup from fish metabolic waste. The chemical formulation 204 is configured to maintain stable pH levels and ionic balance through buffering agents that prevent acidification and maintain vital mineral concentrations, which is required for the live fish cellular function and osmoregulatory processes. The self-regulating properties of the different chemical compounds present in the chemical formulation 204 form a closed-loop water management that preserves water chemistry stability, prevents the accumulation of harmful metabolites, and maintains the water parameters within safe ranges for the live fish health and survival. As a result, the chemical formulation 204 enables complete transportation cycles without water replacement, reducing logistical complexity, minimizing handling stress on the live fish, ensuring consistent water chemistry throughout the transportation, and providing the water quality maintenance that provides the maximum live fish survival rates during the long-distance transport.
[34] In accordance with an embodiment, the oxygen-releasing compounds includes sodium percarbonate granules in a concentration of 10 ppm to 25 ppm and a calcium peroxide in a concentration of 10 ppm to 25 ppm. The sodium percarbonate granules within the oxygen-releasing compounds is configured to decompose in aqueous solution of the chemical formulation 204 to release oxygen through a controlled chemical reaction, providing immediate oxygen availability upon dissolution while maintaining steady oxygen generation over extended periods. In an example, the oxygen-releasing compounds includes the sodium percarbonate granules in a concentration of 10 ppm. In another example, the oxygen-releasing compounds includes the sodium percarbonate granules in a concentration of 20 ppm. In yet another example, the oxygen-releasing compounds includes the sodium percarbonate granules in a concentration of 25 ppm. Furthermore, the calcium peroxide acts as a secondary oxygen source that releases oxygen through a slower sustained reaction, forming a time-release oxygen addition that prevents oxygen depletion during prolonged transportation periods. In an example, the oxygen-releasing compounds includes the calcium peroxide in a concentration of 10 ppm. In another example, the oxygen-releasing compounds includes the calcium peroxide in a concentration 20 ppm. In yet another example, the oxygen-releasing compounds includes the calcium peroxide in a concentration of 25 ppm. The specified concentration ranges of the sodium percarbonate granules and the calcium peroxide ensures the presence of required oxygen levels for the live fish respiration, which prevents oxygen supersaturation that further causes gas bubble disease or other physiological complications. As a result, the oxygen-releasing compound within the specified concentration provides the dissolved oxygen maintenance throughout the long-distance transportation, prevents hypoxic stress conditions, ensures adequate oxygen availability for the live fish respiratory needs, and eliminates the risk of oxygen depletion that further decreases the live fish survival rates.
[35] In accordance with an embodiment, the ammonia control agents include a natural zeolite-clinoptilolite in a concentration of 30 ppm to 100 ppm, and a potassium permanganate in a concentration of 1 ppm to 3 ppm. The natural zeolite-clinoptilolite functions as a selective ion-exchange material with high affinity for ammonium ions, physically capturing and binding ammonia molecules from the water column through the microporous crystal structure and high surface area. In an example, the ammonia control agents include the natural zeolite-clinoptilolite in a concentration of 30 ppm. In another example, the ammonia control agents include the natural zeolite-clinoptilolite in a concentration of 60 ppm. In yet another example, the ammonia control agents include the natural zeolite-clinoptilolite in a concentration of 100 ppm. Furthermore, the potassium permanganate acts as a chemical oxidizing agent that converts toxic ammonia into less harmful nitrogen compounds through oxidation reactions, providing active ammonia neutralization and preventing accumulation of ammonia beyond safe threshold levels. In an example, the ammonia control agents include the potassium permanganate in a concentration of 1 ppm. In another example, the ammonia control agents include the potassium permanganate in a concentration of 2 ppm. In yet another example, the ammonia control agents include the potassium permanganate in a concentration of 3 ppm. The specified concentration ranges of the natural zeolite-clinoptilolite and the potassium permanganate ensure effective ammonia removal while maintaining water chemistry balance and preventing over-treatment that further disrupt beneficial water parameters or harm the live fish health. Additionally, the natural zeolite-clinoptilolite provides continuous ammonia adsorption capacity throughout the transportation period, while the potassium permanganate provides immediate ammonia oxidation response when ammonia levels begin to rise. As a result, the ammonia control agents maintain ammonia concentrations below the 0.5 ppm threshold, prevents ammonia toxicity that can cause gill damage and respiratory distress, ensures safe water quality conditions for the live fish health, and provides ammonia management throughout the extended transportation.
[36] In accordance with an embodiment, the live fish stress reduction compounds include a clove oil in a concentration of 0.10 ppm to 0.25 ppm, vitamin C powder in a concentration of 2 ppm to 4 ppm, and tannic acid powder in a concentration of 0.5 ppm to 1.5 ppm. The clove oil functions as a mild sedative agent containing eugenol that provides calming effects on the live fish nervous system, reducing hyperactivity and erratic swimming behaviours caused by transportation stress while maintaining normal respiratory function. In an example, the live fish stress reduction compounds include the clove oil in a concentration of 0.10 ppm. In another example, the live fish stress reduction compounds include the clove oil in a concentration of 0.15 ppm. In yet another example, the fish stress reduction compounds include the clove oil in a concentration of 0.25 ppm. Furthermore, the vitamin C powder acts as an antioxidant and immune system booster that neutralizes free radicals produced during stress responses, supports immune function maintenance, and enhances the live fish's natural resistance to disease and environmental stressors. In an example, the live fish stress reduction compounds include the Vitamin C powder in a concentration of 2 ppm. In another example, the live fish stress reduction compounds include the Vitamin C powder in a concentration of 3 ppm. In yet another example, the live fish stress reduction compounds include the Vitamin C powder in a concentration of 4 ppm. The tannic acid powder binds excess mucus produced by stressed fish, reduces microbial load in transport water, and provides antioxidant protection against transport-induced cellular damage. In an example, the live fish stress reduction compounds include the tannic acid powder in a concentration of 0.5 ppm. In another example, the live fish stress reduction compounds include the tannic acid powder in a concentration of 1.0 ppm. In yet another example, the live fish stress reduction compounds include the tannic acid powder in a concentration of 1.5 ppm. The specified concentration ranges of the clove oil, the vitamin C powder, and the tannic acid powder ensure therapeutic effectiveness while preventing over-sedation or adverse reactions that affect the live fish health and survival. As a result, the live fish stress reduction compounds significantly reduce the transportation-induced mortality, maintain the normal live fish behaviour and physiological function, enhances the immune system resilience, prevents stress-related diseases, and ensures the live fish arrive at the destination in the required health condition ready for immediate adaptation to new environments.
[37] In accordance with an embodiment, the ionic balance regulators include calcium chloride in a concentration of 5 ppm to 10 ppm, potassium chloride in a concentration of 3 ppm to 6 ppm, magnesium chloride in a concentration of 180 ppm to 250 ppm, and a sodium chloride in a concentration of 2 ppt to 4 ppt. The calcium chloride maintains bone structure, muscle contraction regulation, and cellular membrane stability of the live fish while providing required calcium ions for metabolic processes and gill function. In an example, the ionic balance regulators include the calcium chloride in a concentration of 5 ppm. In another example, the ionic balance regulators include the calcium chloride in a concentration of 7 ppm. In another example, the ionic balance regulators include the calcium chloride in a concentration of 10 ppm. Furthermore, the potassium chloride maintains nerve transmission, muscle function, and cellular osmotic pressure regulation that are required for the normal live fish physiological operations under stress conditions. In an example, the ionic balance regulators include the potassium chloride in a concentration of 3 ppm. In another example, the ionic balance regulators include the potassium chloride in a concentration of 4 ppm. In yet another example, the ionic balance regulators include the potassium chloride in a concentration of 6 ppm. Moreover, the magnesium chloride acts as a cofactor for numerous enzymatic processes, supports protein synthesis, and maintains cellular energy production while contributing to overall water hardness and mineral balance. In an example, the ionic balance regulators include the magnesium chloride in a concentration of 180 ppm. In another example, the ionic balance regulators include the magnesium chloride in a concentration of 200 ppm. In yet another example, the ionic balance regulators include the magnesium chloride in a concentration of 250 ppm. Additionally, the sodium chloride provides fundamental electrolyte balance, enhances osmoregulatory function, and maintains cellular fluid balance that prevents osmotic stress and cellular damage. In an example, the ionic balance regulators include the sodium chloride in a concentration of 2 ppt. In another example, the ionic balance regulators include the sodium chloride in a concentration of 3 ppt. In another example, the ionic balance regulators include the sodium chloride in a concentration of 4 ppt. The specified concentration ranges of the calcium chloride, the potassium chloride, the magnesium chloride, and the sodium chloride replicate the natural freshwater mineral compositions while ensuring the ionic strength for the live fish health and survival. As a result, the ionic balance regulators maintain osmoregulatory function, prevent cellular dehydration and swelling, ensure normal metabolic processes, support the healthy gill function for oxygen uptake, and provide stable electrolyte conditions that enhance the live fish survival rates and post-transportation recovery.
[38] In accordance with an embodiment, the chemical formulation 204 further includes powder iodine in a concentration of 2 ppm to 5 ppm, 2Na-EDTA in a concentration of 2 ppm to 8 ppm, and liquid hydrogen peroxide in a concentration of 1 ppm to 10 ppm for emergency oxygen supplementation. The powder iodine functions as an antimicrobial agent that prevents bacterial growth, controls pathogenic microorganisms, and maintains water sanitation throughout the transportation period while providing mild disinfection without harming the live fish health. In an example, the chemical formulation 204 includes the powder iodine in a concentration of 2 ppm. In another example, the chemical formulation 204 includes the powder iodine in a concentration of 3 ppm. In yet another example, the chemical formulation 204 includes the powder iodine in a concentration of 5 ppm. Furthermore, thee 2Na-EDTA acts as a chelating agent that binds heavy metals and removes toxic metal ions from the water, prevents metal-induced toxicity, and maintains water purity by sequestering harmful contaminants that could accumulate during transportation. In an example, the chemical formulation 204 includes the 2Na-EDTA in a concentration of 2 ppm. In another example, the chemical formulation 204 includes the 2Na-EDTA in a concentration of 6 ppm. In yet another example, the chemical formulation 204 further includes the 2Na-EDTA in a concentration of 8 ppm. Moreover, the liquid hydrogen peroxide provides emergency oxygen supplementation capability through rapid decomposition into water and oxygen, providing immediate dissolved oxygen boost when oxygen levels drop below required thresholds or during equipment malfunction scenarios. In an example, the chemical formulation 204 includes the liquid hydrogen peroxide in a concentration of 1 ppm. In another example, the chemical formulation 204 includes the liquid hydrogen peroxide in a concentration of 5 ppm. In yet another example, the chemical formulation 204 includes the liquid hydrogen peroxide in a concentration of 10 ppm. The specified concentration ranges of the powder iodine, the 2Na-EDTA, and the liquid hydrogen peroxide ensure therapeutic effectiveness while maintaining safety margins that prevent chemical toxicity or adverse reactions in the live fish. As a result, the chemical formulation 204 prevent microbial contamination and disease outbreaks, eliminate heavy metal toxicity risks, provide emergency oxygen support during emergency situations, enhance the overall water quality and safety, and ensure protection against multiple potential hazards during the long-distance live fish transportation.
[39] In accordance with an embodiment, the chemical formulation 204 includes proprietary additives such as a probiotic blend in a concentration of 1 ppm to 3 ppm, an alginate binder in a concentration of 0.5 ppm to 1 ppm, and an herbal extract adaptogen in a concentration of 0.2 ppm to 0.5 ppm. The probiotic blend includes microorganisms that enhances the live fish digestive health, immune system function, and establish positive microbial balance in the aquatic environment while promoting natural disease resistance and reducing pathogenic bacterial growth. In an example, the chemical formulation 204 includes proprietary additives, such as the probiotic blend in a concentration of 1 ppm. In another example, the chemical formulation 204 includes proprietary additives, such as the probiotic blend in a concentration of 2 ppm. In yet another example, the chemical formulation 204 includes proprietary additives, such as the probiotic blend in a concentration of 3 ppm. Furthermore, the alginate binder functions as a natural stabilizing agent that maintains formulation integrity, controls compound release rates, and prevents precipitation or separation of active ingredients while ensuring consistent distribution and bioavailability of chemical components throughout the water column. In an example, the chemical formulation 204 includes the alginate binder in a concentration of 0.5 ppm. In another example, the chemical formulation 204 includes the alginate binder in a concentration of 1 ppm. In yet another example, the chemical formulation 204 includes the alginate binder in a concentration of 0.75 ppm. Moreover, the herbal extract adaptogen provides natural stress adaptation that enhances the live fish resilience to environmental changes, supports physiological adaptation mechanisms, and promotes natural stress recovery responses while maintaining overall fish vitality and health. In an example, the chemical formulation 204 includes the herbal extract adaptogen in a concentration of 0.2 ppm. In another example, the chemical formulation 204 includes the herbal extract adaptogen in a concentration of 0.3 ppm. In yet another example, the chemical formulation 204 includes the herbal extract adaptogen in a concentration of 0.5 ppm. The specified concentration ranges of the probiotic blend, the alginate binder, and the herbal extract adaptogen is configured to optimize biological activity while ensuring compatibility with the other chemical formulation components and maintaining the live fish safety throughout the transportation period. As a result, the proprietary additives enhance the live fish immune function and disease resistance, enhance formulation stability and effectiveness, promote natural stress adaptation and recovery, provide comprehensive biological support for the live fish health, and ensure the balanced chemical formulation throughout the extended transportation periods while maintaining consistent therapeutic benefits.
[40] The diagram 200 further represents the sequential process of the live fish transportation for long distances. The diagram 200 also includes operations 206 to 210. At operation 206, the transport tank 102 contains freshly harvested live fish that are loaded and prepared for long-distance transportation, with the plurality of fishes 202 within the aquatic environment of transport tank 102 representing the initial live fish population in required health condition ready for long-distance transportation. At operation 208, the chemical formulation is sprinkled into the water inside the transport tank 102, and after covering a distance of around thousands of kilometres, the transport tank 102 reaches at a point where the active transportation phase occurs with integrated chemical formulation management is actively maintaining water quality, showing visible dosing indicators that illustrates continuous addition of the oxygen-releasing compounds, the ammonia control agents, and the stress reduction compounds into the aquatic environment based on real-time monitoring by the controller 104 and the water quality sensors 106. At operation 210, the same transport tank 102 reaches the final destination where the live fish arrive in required health condition with the live fish symbols clearly visible throughout the water medium, indicating survival rates and readiness for unloading and adaptation to the new environment after completing the long-distance. Throughout the sequential process, the controller 104 and the water quality sensors 106 continuously monitors the dissolved oxygen levels, the pH balance, the temperature variations, and the ammonia concentrations within the transport tank 102, ensuring automated chemical dosing when parameters deviate from safe thresholds to maintain dissolved oxygen above 4 mg/L and ammonia below 0.5 ppm during the transportation phase at operation 208. As a result, the system 100 achieves 95% survival rate, thereby reduces operational complexity, minimizes environmental impact, and provides sustainable transportation for commercial aquaculture operations.
[41] Advantageously, the chemical formulation 204 utilized in system 100 is configured to maintain the water quality during long-distance live fish transport through a synergistic blend of the oxygen-releasing compounds, the ammonia control agents, the live fish stress-reduction compounds, and the ionic balance regulators. Moreover, the oxygen-releasing compounds ensure a sustained supply of the dissolved oxygen throughout the transportation, while the ammonia control agents adsorb or neutralize toxic nitrogenous wastes, maintaining water safety. Furthermore, the inclusion of the stress-reducing agents, such as herbal sedatives and antioxidants allows for lowering the live fish cortisol levels, thereby minimizing physiological distress. Furthermore, the ionic balance regulators, such as calcium, potassium, and magnesium chlorides maintain osmotic equilibrium, enhancing the live fish metabolism under confined transport conditions. Additionally, the chemical formulation 204 eliminates the requirement for frequent water exchanges by stabilizing key parameters within safe thresholds, thereby reducing operational complexity, water usage, and environmental impact, while significantly enhancing the live fish survival rates over distances of up to thousands of kilometres.
[42] FIG.3 is a flowchart of method for long-distance transport of live fish, in accordance with an embodiment of the present disclosure. FIG. 3 is described in conjunction with the elements of the FIGs. 1 and 2. With reference to FIG. 3, there is shown a flowchart of a method 300 for long-distance transport of live fish that includes steps 302 to 310. The system 100 is configured to execute the method 300.
[43] At step 302, the method 300 includes harvesting the live fish from culture environment, which involves the extraction and collection of the live fish (or the plurality of fishes 202) from the established culture environment by utilizing harvesting techniques that reduces stress responses and prevent environmental shock that could affect the live fish health during the transition from culture to transportation phases. Furthermore, the harvesting process ensures the live fish handling procedures preserve natural physiological states, maintain the live fish immune function, and prevent injury or damage that could lead to mortality during subsequent transport operations, thereby providing the live fish for long-distance transportation with enhanced condition and vitality.
[44] At step 304, the method 300 includes loading harvested the live fish into the transport tank, which involves the systematic transfer of the harvested live fish into the transport tank 102 by utilizing controlled loading procedures that minimize handling stress, prevent overcrowding conditions, and ensure the live fish distribution within a containment area while maintaining the live fish density ratios. Furthermore, the loading of the harvested fish into the transport tank provides adequate swimming space for the live fish movement, prevents territorial conflicts between the live fish, and ensures adequate water volume per live fish for maintaining healthy conditions throughout the transportation, thereby preserving the live fish health and physiological stability.
[45] At step 306, the method 300 includes monitoring the water quality parameters using the water quality sensors 106 connected to the controller 104, which involves the continuous real-time assessment and measurement of the water quality parameters, such as the dissolved oxygen levels, the pH balance, the temperature variations, and the ammonia concentrations by utilizing the water quality sensors 106 that transmit measurement data directly to the controller 104 for immediate analysis and response determination. Furthermore, the monitoring at step 306 operates continuously throughout the long-distance transportation to detect the water quality parameters fluctuations before the quality water parameters reach dangerous levels, enabling proactive intervention and maintenance of the aquatic conditions, thereby ensuring early detection of the water quality parameters issue and providing reliable data foundation for the system 100 responses during the live fish transportation.
[46] At step 308, the method 300 includes adding the chemical formulation 204 to the transport tank 102 based on the monitored water quality parameters, which involves the systematic automated addition of the different chemical compounds of the chemical formulation 204, such as the oxygen-releasing compounds, the ammonia control agents, the live fish stress reduction compounds, and the ionic balance regulators based on the water quality conditions detected by the monitoring and processed by the controller 104. Furthermore, the addition of the chemical formulation 204, which responds to specific parameter thresholds, such as, the dissolved oxygen falling below 4 mg/L, the ammonia concentration exceeding 0.5 ppm, ensuring the chemical intervention addresses the water quality issues without over-treatment, thereby maintaining the aquatic conditions through the management of the addition of the chemical formulation that enhances the live fish health and survival throughout the long distance transportation.
[47] At step 310, the method 300 includes transporting the live fish without requiring water exchange during transport, which involves the execution of the long-distance live fish transportation by utilizing the chemical formulation 204 that maintains the water quality and stability of the formulated water throughout the entire transportation without the requirement for external water replacement or exchange procedures. Furthermore, the transportation of the live fish relies on the synergistic effect of the addition of the chemical formulation 204 the water quality maintenance capabilities to preserve the aquatic conditions from origin to destination, eliminating logistical complications associated with water sourcing and replacement. Moreover, the transportation process preventing environmental disruption, thereby enabling sustainable transportation that provides the maximum live fish survival rates over extended distances while minimizing operational complexity and environmental impact on the health of the live fish.
[48] In accordance with an embodiment, adding the chemical formulation 204 includes adding additional oxygen-releasing compounds when dissolved oxygen falls below 4 mg/L, adding additional ammonia control agents when ammonia concentration exceeds 0.5 ppm and adding additional stress reduction compounds during periods of handling stress. In an implementation, the method 300 includes the automated addition of the oxygen releasing compounds, such as the sodium percarbonate granules and the calcium peroxide through the automatic dosing module 110 when dissolved oxygen measurements from the water quality sensors 106 indicate levels below the 4 mg/L threshold that could affect the live fish respiratory function and survival, the method 300 ensures immediate oxygen supplementation to prevent hypoxic stress conditions within the transport tank 102. Furthermore, the method 300 includes automated addition of the natural zeolite-clinoptilolite and the potassium permanganate when the ammonia concentration monitoring detects levels exceeding the toxic 0.5 ppm threshold that may result to gill damage and physiological stress, the method 300 provides the immediate ammonia neutralization and removal capabilities to maintain safe water conditions. Moreover, the stress reduction method 300 includes the automated addition of the clove oil, the vitamin C powder, and the tannic acid powder during periods of the handling stress, environmental changes, or behavioural indicators of the live fish distress detected through the water quality sensors 106 of the system 100, ensuring immediate stress mitigation for maintaining the live fish health and welfare the long distance transportation. As a result, the chemical addition in the method 300 provides immediate automated response to the water quality conditions, ensures intervention based on actual parameter measurements and predetermined thresholds, prevents the live fish mortality through proactive automated chemical management, maintains water conditions through the threshold-based dosing protocols, and delivers the automated chemical intervention that provides the maximum live fish survival rates during the long distance transportation operations.
[49] Advantageously, the method 300 for the long-distance transport of the live fish provides systematic live fish preparation and the automated water quality management throughout the transportation without requiring water replacement procedures. The harvesting and loading processes ensure gentle live fish handling that minimizes stress while maintaining the live fish density ratios within the transport tank. The continuous monitoring of the water quality parameters, such as the dissolved oxygen, the pH, the temperature, and the ammonia levels enables real-time assessment and immediate response through the automated chemical formulation addition when parameters deviate from safe thresholds. The method 300 further eliminates the requirement for water exchange procedures that typically require 50% water replacement every 30 kilometres, thereby reducing operational complexity and environmental impact while maintaining consistent aquatic conditions from origin to destination. As a result, the method 300 provides up to 95% live fish survival rates over distances.
[50] The steps 302 to 310 are only illustrative, and other alternatives can also be provided where one or more steps are added, one or more steps are removed, or one or more steps are provided in a different sequence without departing from the scope of the claim herein.
[51] FIG. 4 is a graphical representation illustrating the water quality parameter maintenance during long-distance live fish transport, in accordance with an embodiment of the present disclosure. FIG. 4 is described in conjunction with the elements of the FIGs. 1, 2, and 3. With reference to FIG. 4, there is shown the graphical representation 400, which provides the comparison of the water quality parameters before and after 1000 km transportation by utilizing the chemical formulation 204 from the system 100, measured on the different water quality parameters (i.e., the dissolved oxygen (i.e., in mg/L), the pH levels, and the ammonia (i.e., in ppm)). The different water quality parameters are shown along the abscissa axis 402, while the parameter values are measured along the ordinate axis 404 ranging from 0 to 8 units. The graphical representation 400 includes a plurality of bars with diagonal line patterns for initial values and dotted patterns for final values depicting various changes in the water quality parameters before and after long-distance transportation by utilizing the chemical formulation for the dissolved oxygen (mg/L), the pH levels, and the ammonia concentration (ppm).
[52] The graphical representation 400 includes the bar 406A and the bar 408A depicting the water quality parameter of the dissolved oxygen levels, with the bar 406A representing approximately 7.2 mg/L initially and the bar 408A showing approximately 5.8 mg/L finally, representing the sustained dissolved oxygen availability above the 4 mg/L threshold throughout the 1000km transportation. Furthermore, the bars 406B and 408B depict the water quality parameter of the pH levels, with the bar 406B showing approximately 7.5 initially and the bar 408B showing approximately 7.3 finally, indicating the pH maintenance within the physiological range of 7.2-7.5 for the live fish welfare during extended transportation. Moreover, the bar 406C and the 408C represents the water quality parameter of the ammonia concentration, with the bar 406C showing approximately 0.2 ppm initially and the bar 408C showing approximately 0.4 ppm finally, illustrating the ammonia control well below the toxic 0.5 ppm threshold throughout the transportation.
[53] Therefore, the graphical representation 400 indicates that all the water quality parameters (i.e., the dissolved oxygen (i.e., in mg/L), the pH levels, and the ammonia (i.e., in ppm)) remained within ranges following 1000km transportation by utilizing the chemical formulation from the system 100, reflecting the synergistic action of the oxygen-releasing compounds, the ammonia control agents, the live fish stress reduction compounds, and the ionic balance regulators throughout the journey. The sustained water quality parameter maintenance illustrates the effectiveness of the chemical formulation in preserving the aquatic conditions without water exchange, directly correlating with the enhanced 95% live fish survival rate over 1000km distances within 24 hours and long-distance live fish transportation operations.
[54] FIG. 5 is a graphical representation illustrating the chemical formulation component effectiveness during the live fish transportation, in accordance with an embodiment of the present disclosure. FIG. 5 is described in conjunction with the elements of the FIGs. 1, 2, 3, and 4. With reference to FIG. 5, there is shown the graphical representation 500, which provides the effectiveness percentages of the different chemical formulation components from the system 100, measured on various component categories (i.e., Zeolite + EDTA (Ammonia Control), Oxygen Compounds (DO Maintenance), Clove Oil + Vitamin C (Stress Reduction), Ion Balance (Osmoregulation), Probiotics (Water Quality), and Overall System (Combined Effect)). The different chemical formulation component categories are represented along the abscissa axis 502, while the effectiveness percentages are measured along the ordinate axis 504 ranging from 0 to 100%. The graphical representation 500 includes a plurality of bars with different patterns depicting various effectiveness levels of the chemical formulation components for the ammonia control, the dissolved oxygen maintenance, the stress reduction, the osmoregulation, the water quality enhancement, and the overall system performance.
[55] The graphical representation 500 includes a bar 506 depicting the effectiveness of Zeolite + EDTA for the ammonia control representing approximately 85% effectiveness, illustrating the strong ammonia neutralization and removal capabilities throughout transportation. Furthermore, the bar 508 represents the Oxygen Compounds for DO maintenance showing approximately 95% effectiveness, indicating the enhanced dissolved oxygen level maintenance above the thresholds during long-distance transport. Moreover, the bar 510 depicts the clove Oil + Vitamin C for the stress reduction showing approximately 50% effectiveness, representing moderate stress mitigation and immune system support for the live fish welfare. Furthermore, the bar 512 represents ion Balance for the osmoregulation representing approximately 90% effectiveness, illustrating electrolyte balance maintenance for the live fish cellular function. Furthermore, the bar 514 depicts the probiotics for the water quality showing approximately 75% effectiveness, representing good microbial balance and the water quality enhancement. Additionally, the bar 516 represents the overall system combined effect showing approximately 95% effectiveness, illustrating the synergistic performance of all the chemical formulation components.
[56] Therefore, the graphical representation 500 represents that the various chemical compounds of the chemical formulation 204 attains effectiveness levels ranging from 50% to 95%, with the oxygen compounds and the overall system performance showing the highest effectiveness at 95%, reflecting the concentration ranges and the interactions between the sodium percarbonate granules, the calcium peroxide, the natural zeolite-clinoptilolite, the potassium permanganate, the clove oil, the vitamin C powder, and the ionic balance regulators. The comprehensive chemical component effectiveness illustrates the chemical formulation's capability to maintain the water quality parameters, enhances the live fish health and welfare, and attains the 95% survival rate over 1000 km transportation distances up to 24 hours through the integrated chemical formulation management without requiring water exchange during transport.
[57] FIG. 6 is a graphical representation illustrating the survival rate performance at different transport distances by utilizing the chemical formulation system, in accordance with an embodiment of the present disclosure. FIG. 6 is described in conjunction with the elements of the FIGs. 1, 2, 3, 4, and 5. With reference to FIG. 6, there is shown the graphical representation 600, which provides the live fish survival rate percentages at various transportation distances (i.e., 250km, 500km, 750km, and 1000km). The different transport distances are represented along the abscissa axis 602, while the survival rate percentages are measured along the ordinate axis 604 ranging from 94.0% to 98.0%. The graphical representation 600 includes a plurality of bars with dotted patterns depicting the live fish survival rates achieved at increasing transportation distances representing the effectiveness of the chemical formulation system over extended transportation.
[58] The graphical representation 600 includes a bar 606 depicting the survival rate at 250km transport distance showing approximately 97.2% live fish survival, representing excellent performance during short to medium distance transportation with the chemical formulation effectiveness. Furthermore, the bar 608 represents the survival rate at 500km transport distance showing approximately 96.5% live fish survival, indicating sustained high performance during medium distance transportation with the enhanced water quality maintenance. Moreover, the bar 610 depicts the survival rate at 750km transport distance represents approximately 95.8% live fish survival, illustrating reliable performance during extended distance transportation with the continued chemical formulation efficacy. Additionally, the bar 612 represents the survival rate at 1000km transport distance showing approximately 95.7% live fish survival, representing enhanced survival rate during maximum distance transportation while maintaining the water quality parameters throughout the transportation.
[59] Therefore, the graphical representation 600 represents that the chemical formulation consistently maintains the live fish survival rates above 95% across all the transport distances from 250km to 1000km, with only a gradual decline from 97.2% to 95.7% over the full distance range, reflecting the sustained effectiveness of the oxygen-releasing compounds, the ammonia control agents, the live fish stress reduction compounds, and the ionic balance regulators throughout the extended transportation. The consistent high survival rate performance demonstrates the chemical formulation's capability to maintain the aquatic conditions without water exchange, achieving the 95% minimum survival rate even at maximum 1000km transportation distances within 24 hours through the integrated automated chemical management and the real-time water quality monitoring.
[60] FIG. 7 is a graphical representation illustrating the water usage efficiency (cumulative percentage over distance) during the live fish transportation, in accordance with an embodiment of the present disclosure. FIG. 7 is described in conjunction with the elements of the FIGs. 1, 2, 3, 4, 5, and 6. With reference to FIG. 7, there is shown the graphical representation 700, which provides the cumulative water usage percentages across different distance segments (i.e., 0-250km, 250-500km, 500-750km, and 750-1000km) demonstrating the water efficiency attained by the chemical formulation from the system 100 without requiring water exchange during transport. The different distance segments are expressed along the abscissa axis 702, while the water usage percentages are measured along the ordinate axis 704 ranging from 0% to 10%. The graphical representation 700 includes a plurality of bars with diagonal line patterns depicting the minimal water usage requirements at each distance segment, emphasizing the sustainable transportation.
[61] The graphical representation 700 includes a bar 706 depicting the water usage for 0-250km distance segment showing approximately 1% water usage, representing minimal water consumption during initial transportation phase with the chemical formulation efficiency. Furthermore, the bar 708 represents the water usage for 250-500km distance segment represents approximately 2% water usage, indicating continued low water consumption during medium distance transportation with sustained chemical effectiveness. Moreover, the bar 710 depicts the water usage for 500-750km distance segment representing approximately 3% water usage, demonstrating maintained water efficiency during extended distance transportation with enhanced chemical formulation management. Additionally, the bar 712 represents water usage for 750-1000km distance segment representing approximately 5% water usage, indicating the highest but still minimal water consumption during maximum distance transportation while maintaining the water quality parameters throughout the transportation.
[62] Therefore, the graphical representation 700 indicates that the chemical formulation attains exceptional water usage efficiency with cumulative water usage remaining below 5% even at maximum 1000 km transportation distances, representing the significant enhancement over the conventional methods that require 50% water replacement every 30km, reflecting the sustained effectiveness of the oxygen-releasing compounds, the ammonia control agents, and the ionic balance regulators that eliminate the requirement for water exchange procedures. The progressive but minimal increase in water usage from 1% to 5% across distance segments represents the chemical formulation's capability to maintain optimal aquatic conditions through the integrated chemical management, attaining sustainable transportation that minimize environmental impact, reduce operational costs, and provide reliable water quality maintenance throughout the extended transportation without compromising the live fish survival rates.
[63] FIG. 8 is a graphical representation illustrating the water parameter stability during 24-hour transport, in accordance with an embodiment of the present disclosure. FIG. 8 is described in conjunction with the elements of the FIGs. 1, 2, 3, 4, 5, 6, and 7. With reference to FIG. 8, there is shown the graphical representation 800, which provides the parameter stability percentages for the dissolved oxygen (DO), the pH levels, and the ammonia (NH3) control across different time periods (i.e., 0-4 hours, 4-8 hours, 8-12 hours, 12-16 hours, 16-20 hours, and 20-24 hours) demonstrating the sustained effectiveness of the chemical formulation from the system 100 throughout the complete transportation cycle. The different time periods are represented along the abscissa axis 802, while the parameter stability percentages are measured along the ordinate axis 804 ranging from 0% to 100%. The graphical representation 800 includes three distinct bar patterns representing DO stability (crosshatch pattern), pH stability (dotted pattern), and NH3 control (diagonal line pattern) showing the temporal performance of each water quality parameter during extended transportation.
[64] The graphical representation 800 includes a bars 806A, 806B, and 806C depicting parameter stability for 0-4 hours showing approximately 100% DO stability, 100% pH stability, and 100% NH3 control respectively, representing initial performance with maximum chemical formulation effectiveness. Furthermore, the bars 808A, 808B, and 808C represent parameter stability during 4-8 hours showing approximately 95% DO stability, 100% pH stability, and 85% NH3 control respectively, indicating sustained high performance with slight decline in the dissolved oxygen and the ammonia control. Moreover, the bars 810A, 810B, and 810C depict parameter stability during 8-12 hours represents approximately 90% DO stability, 95% pH stability, and 75% NH3 control respectively, demonstrating continued effectiveness with gradual parameter stability reduction. Moreover, the bars 806D, 808D, and 810D represent parameter stability during 12-16 hours showing approximately 85% DO stability, 95% pH stability, and 60% NH3 control respectively. Furthermore, the bars 806E, 808E, and 810E depict parameter stability during 16-20 hours showing approximately 82% DO stability, 95% pH stability, and 50% NH3 control respectively. Additionally, the bars 806F, 808F, and 810F represent parameter stability during 20-24 hours showing approximately 80% DO stability, 95% pH stability, and 40% NH3 control respectively, indicating maintained parameter levels even at maximum transport duration.
[65] Therefore, the graphical representation 800 indicates that the system 100 maintains the water parameter stability throughout the complete 24-hour transportation cycle, with the pH stability remaining consistently above 95%, the dissolved oxygen stability declining gradually from 100% to 80% but remaining well above the predefined thresholds, and the NH3 control decreasing from 100% to 40% while still providing the ammonia management, reflecting the time-release properties and sustained effectiveness of the oxygen-releasing compounds, the pH buffers, and the ammonia control agents throughout extended transportation. The temporal stability performance illustrates the chemical formulation's capability to maintain the live fish-safe water conditions for the complete 1000km transportation duration within 24 hours, ensuring dissolved oxygen levels remain above 4 mg/L, pH stays within 7.2-7.5 range, and ammonia concentrations remain below toxic thresholds, thereby attaining the 95% the live fish survival rate through the automated water quality management without requiring water exchange procedures.
EXPERIMENTAL PART
Example 1: Chemical Formulation Preparation and Testing
Materials Used: Sodium percarbonate granules, Calcium peroxide, Natural zeolite-clinoptilolite, Potassium permanganate, Clove oil, Vitamin C powder, Tannic acid powder, Calcium chloride, Potassium chloride, Magnesium chloride, Sodium chloride, Powder iodine, 2Na-EDTA, Liquid hydrogen peroxide, Transport tank, Water quality sensors, Controller, Automatic dosing module.
Steps for Chemical Formulation Preparation:
1. Oxygen-Releasing Compound Preparation
i) Sodium percarbonate granules are measured at concentrations ranging from 10 ppm to 25 ppm and dissolved in distilled water under controlled mixing conditions.
ii) Calcium peroxide is prepared separately at concentrations ranging from 10 ppm to 25 ppm and combined with the sodium percarbonate solution to form the oxygen-releasing compound mixture.
iii) The dual oxygen-release mechanism is verified by monitoring dissolved oxygen levels over a 24-hour period, ensuring sustained oxygen availability above 4 mg/L.
2. Ammonia Control Agent Preparation
i) Natural zeolite-clinoptilolite is prepared at concentrations ranging from 30 ppm to 100 ppm and mixed thoroughly to ensure uniform distribution.
ii) Potassium permanganate solution is prepared at concentrations ranging from 1 ppm to 3 ppm and slowly added to the zeolite mixture under constant stirring.
iii) The ammonia control effectiveness is tested by introducing controlled ammonia concentrations and monitoring reduction below 0.5 ppm threshold.
3. Fish Stress Reduction Compound Preparation
i) Clove oil is diluted to concentrations ranging from 0.10 ppm to 0.25 ppm using appropriate carrier solutions.
ii) Vitamin C powder is dissolved at concentrations ranging from 2 ppm to 4 ppm in temperature-controlled water.
iii) Tannic acid powder is prepared at concentrations ranging from 0.5 ppm to 1.5 ppm and combined with the clove oil and vitamin C solutions.
4. Ionic Balance Regulator Preparation
i) Individual salt solutions are prepared: calcium chloride (5-10 ppm), potassium chloride (3-6 ppm), magnesium chloride (180-250 ppm), and sodium chloride (2-4 ppt).
ii) The salt solutions are combined in ratios and mixed thoroughly to form the ionic balance regulator mixture.
iii) Osmotic pressure and conductivity measurements are performed to verify ionic balance maintenance.
[66] Modifications to embodiments of the present disclosure described in the foregoing are possible without departing from the scope of the present disclosure as defined by the accompanying claims. Expressions such as "including", "comprising", "incorporating", "have", "is" used to describe, and claim the present disclosure are intended to be construed in a non-exclusive manner, namely allowing for items, components or elements not explicitly described also to be present. Reference to the singular is also to be construed to relate to the plural. The word "exemplary" is used herein to mean "serving as an example, instance or illustration". Any embodiment described as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments and/or to exclude the incorporation of features from other embodiments. The word "optionally" is used herein to mean "is provided in some embodiments and not provided in other embodiments". It is appreciated that certain features of the present disclosure, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the present disclosure, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable combination or as suitable in any other described embodiment of the disclosure.
,CLAIMS:CLAIMS
I/We claim:
1. A chemical formulation (204) for long-distance live fish transport, the chemical formulation comprising:
oxygen-releasing compounds;
ammonia control agents;
fish stress reduction compounds; and
ionic balance regulators, wherein the chemical formulation (204) is configured to maintain water quality during the live fish transport without requiring water exchange during transport.
2. The chemical formulation (204) as claimed in claim 1, wherein the oxygen-releasing compounds comprise sodium percarbonate granules in a concentration of 10 ppm to 25 ppm and calcium peroxide in a concentration of 10 ppm to 25 ppm.
3. The chemical formulation (204) as claimed in claim 1, wherein the ammonia control agents comprise natural zeolite-clinoptilolite in a concentration of 30 ppm to 100 ppm, and potassium permanganate in a concentration of 1 ppm to 3 ppm.
4. The chemical formulation (204) as claimed in claim 1, wherein the live fish stress reduction compounds comprise clove oil in a concentration of 0.10 ppm to 0.25 ppm, vitamin C powder in a concentration of 2 ppm to 4 ppm, and tannic acid powder in a concentration of 0.5 ppm to 1.5 ppm.
5. The chemical formulation (204) as claimed in claim 1, wherein the ionic balance regulators comprise calcium chloride in a concentration of 5 ppm to 10 ppm, potassium chloride in a concentration of 3 ppm to 6 ppm, magnesium chloride in a concentration of 180 ppm to 250 ppm, and sodium chloride in a concentration of 2 ppt to 4 ppt.
6. The chemical formulation (204) as claimed in claim 1, further comprising powder iodine in a concentration of 2 ppm to 5 ppm, 2Na-EDTA in a concentration of 2 ppm to 8 ppm, and liquid hydrogen peroxide in a concentration of 1 ppm to 10 ppm for emergency oxygen supplementation.
7. The chemical formulation (204) as claimed in claim 1, further comprising proprietary additives comprising a probiotic blend in a concentration of 1 ppm to 3 ppm, an alginate binder in a concentration of 0.5 ppm to 1 ppm, and an herbal extract adaptogen in a concentration of 0.2 ppm to 0.5 ppm.
8. A system (100) for long-distance transport of live fish comprising:
a transport tank (102);
water quality sensors (106) configured to monitor dissolved oxygen, pH, temperature, and ammonia levels;
a controller (104) configured to process real-time water quality data;
an automatic dosing module (110) operatively connected to the controller (104), wherein the controller (104) is configured to activate the automatic dosing module (110) to supply the chemical formulation (204) based on the monitored water quality data.
9. A method (300) for long-distance transport of live fish, the method (300) comprising:
harvesting the live fish from a culture environment;
loading the harvested live fish into a transport tank;
monitoring water quality parameters using water quality sensors (106) connected to a controller;
adding a chemical formulation (204) to the transport tank based on the monitored water quality parameters; and
transporting the live fish without requiring water exchange during transport.
10. The method (300) as claimed in claim 9, wherein adding the chemical formulation (204) comprises:
adding additional oxygen-releasing compounds when dissolved oxygen falls below 4 mg/L;
adding additional ammonia control agents when ammonia concentration exceeds 0.5 ppm; and
adding additional stress reduction compounds during periods of handling stress.