Description:FIELD OF THE INVENTION

[0001] The present invention relates to a microbe collection and analysis device that is capable of exploring and collecting microbial samples from various environments by detecting and identifying specific biological entities, and adapts to navigate through spaces while managing and preserving the samples, thereby making it an efficient solution for environmental monitoring and research.

BACKGROUND OF THE INVENTION

[0002] Microorganisms are the cornerstone of life on Earth, playing a vital role in ecosystems, human health, and the environment. However, understanding and characterizing microbial communities in various environments remains a significant challenge. The ability to efficiently explore, collect, and analyze microbial samples is crucial for advancing environmental monitoring, research, and applications in fields like biotechnology and medicine.

[0003] Traditional methods for collecting and analyzing microbial samples involve labor-intensive processes such as manual sampling, culturing, and sequencing. These techniques often rely on specialized equipment, trained personnel, and extensive laboratory resources. Additionally, they might require sample transportation, storage, and handling, which lead to contamination, degradation, or loss of samples.

[0004] CN201177604Y discloses a microscopic microbe image detection and recognition device can effectively record and recognize microbes parasitized on grains and foods. The device comprises microscopic microbe image detection equipment and microscopic microbe image recognition equipment, wherein the microscopic microbe image detection equipment comprises a CCD camera, an image collection card, an optical microscope and a uniform illumination chamber; the image of a microbe sample is formed by the optical microscope; the CCD camera serving as the image collection device in the microscopic microbe image detection equipment is connected with the image collection card and converts an optical signal of the image into an electrical signal; the image collection card is connected with the microscopic microbe image recognition equipment; and the CCD camera is arranged in the uniform illumination chamber.

[0005] US4945060A discloses an instrument and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor means with an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO2 concentration in the medium are detected from outside the vessel.

[0006] Conventionally, there exists many devices that are capable of collecting and performing analysis of microbial samples, however these existing devices fail in providing a means to detect microbial activity and identifying type of microbe present. In addition, these existing devices also fail in providing a means to avoid obstacles, and manage collected samples to preserve their integrity.

[0007] In order to overcome the aforementioned drawbacks, there exists a need in the art to develop a device that requires to be capable of collecting and analysing microbial samples by monitoring activity and type of the microbes. Furthermore, the developed device requires to be potent enough of avoiding obstacles, and also manages collected samples in view of preserving their integrity.

OBJECTS OF THE INVENTION

[0008] The principal object of the present invention is to overcome the disadvantages of the prior art.

[0009] An object of the present invention is to develop a device that is capable of collecting and analyzing microbial samples from various environments by detecting microbial activity, and identifying the type of microbes present.

[0010] Another object of the present invention is to develop a device that is capable of navigating through spaces, avoid obstacles, and manage collected samples to preserve their integrity for further analysis.

[0011] Yet another object of the present invention is to develop a device that is capable of providing a user-friendly interface, enabling individuals to initiate sampling and analysis processes and visualize the results, making it an efficient and informative solution for environmental analysis.

[0012] The foregoing and other objects, features, and advantages of the present invention will become readily apparent upon further review of the following detailed description of the preferred embodiment as illustrated in the accompanying drawings.

SUMMARY OF THE INVENTION

[0013] The present invention relates to a microbe collection and analysis device that is capable of characterizing microbial life in diverse environments through identification and adaptation to specific biological signatures, expertly navigating and preserving samples for in-depth analysis, ultimately streamlining environmental monitoring and research with precision and ease.

[0014] According to an embodiment of the present invention, a microbe collection and analysis device, comprising a cuboidal housing equipped with tracked wheels beneath the housing that enable it to move and navigate through various environments, an artificial intelligence-powered imaging unit is installed on the housing, allowing it to record and process images of its surroundings, detect obstacles, and chart pathways to avoid collisions, a pair of clamps, mounted on four-bar linkage mechanisms, which are used to collect organic samples, which are then stored in a box within the housing, and a built-in GPS (global positioning system)unit records the location from which they were collected, an electrochemical biosensor embedded in one of the clamps detects microbial activity in the samples, a chamber with an articulated telescopic gripper, which picks up the samples and places them on a conveyor belt, transporting them to a container within the housing, the container is equipped with a primary Peltier unit and water sprayer, maintaining optimal temperature and moisture levels for the samples, an articulated arm places the samples onto an agar plate, provided within the housing, for further analysis.

[0015] According to another embodiment of the present invention, the proposed device further comprises of an artificial intelligence-based camera, installed in the housing to record and process images of the vicinity to determine the position of the slide held by the arm and actuates an articulate L-shaped telescopic bar with a swab at its end to smudge the sample on the slide, the arm then places the slide against the flame of the burner to establish the type of bacteria based on the change in color of the flame, a tank with a primary nozzle to store a catalyst, which is dispensed onto the sample in a tray, the camera detects bubbling in the sample to determine the presence of oxygen release from microbes, a compartment with a secondary nozzle contains an enzyme to be dispensed onto the agar plate, allowing the camera to detect clear zones and determine starch hydrolysis, an articulated L-shaped telescopic link with a receptacle at its end contains LCB (lactophenol cotton blue) to drip onto the tray with the sample, a sectioned vessel located within the housing, with differing chemical environments, for storage and preservation of samples based on the type of microbe detected, a temperature sensor is embedded in the chamber to detect the temperature of the sample and regulate the operation of the primary Peltier unit, a moisture sensor is embedded in the chamber to detect the moisture level of the sample and regulate the operation of the water sprayer, a touch-enabled display unit is disposed on the housing to enable the user to initiate sample analysis and display results of analyses performed, a secondary Peltier units are configured with the vessel to maintain the temperature of the vessel based on the type of microbe stored.

[0016] While the invention has been described and shown with particular reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

[0017] These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:
Figure 1 illustrates an isometric view of a microbe collection and analysis device.

DETAILED DESCRIPTION OF THE INVENTION

[0018] The following description includes the preferred best mode of one embodiment of the present invention. It will be clear from this description of the invention that the invention is not limited to these illustrated embodiments but that the invention also includes a variety of modifications and embodiments thereto. Therefore, the present description should be seen as illustrative and not limiting. While the invention is susceptible to various modifications and alternative constructions, it should be understood, that there is no intention to limit the invention to the specific form disclosed, but, on the contrary, the invention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the invention as defined in the claims.

[0019] In any embodiment described herein, the open-ended terms "comprising," "comprises,” and the like (which are synonymous with "including," "having” and "characterized by") may be replaced by the respective partially closed phrases "consisting essentially of," consists essentially of," and the like or the respective closed phrases "consisting of," "consists of, the like.

[0020] As used herein, the singular forms “a,” “an,” and “the” designate both the singular and the plural, unless expressly stated to designate the singular only.

[0021] The present invention relates to a microbe collection and analysis device that is capable of providing a user-friendly interface, enabling individuals to initiate sampling and analysis processes and visualize the results, making it an efficient and informative solution for environmental analysis.

[0022] Referring to Figure 1, an isometric view of a microbe collection and analysis device is illustrated, comprising a cuboidal housing 101 having a pair of tracked wheels underneath the housing 101, an artificial intelligence-based imaging unit 103, installed on the housing 101, a pair of clamps 104 mounted with the housing 101 by means of four-bar linkage mechanisms 105, a chamber 106 disposed within the housing 101, an articulate telescopic gripper 107 mounted on a dual axis lead screw mechanism 108 located within the housing 101, a conveyor belt 109 to convey the sample into a container 110 within the housing 101, an articulated arm 111 disposed within the housing 101 and an agar plate 112 provided within the housing 101.

[0023] Figure 1 further illustrates an artificial intelligence-based camera 113, installed in the housing 101, an articulate L-shaped telescopic bar 114 having a swab 115 at an end, a tank 116, configured with a primary nozzle 117, provided within the housing 101, a compartment 118, configured with a secondary nozzle 119, an articulated L-shaped telescopic link 120, having a receptacle 121 at an end, a sectioned vessel 122 located within the housing 101, a touch-enabled display unit 123 disposed on the housing 101, a tray 124 installed in the housing 101 and a box 102 within the housing 101.

[0024] The device disclosed herein, comprises of a cuboidal housing 101, which serves as a main structure of the device and developed to be positioned on a ground surface, wherein the housing 101 arranged with a pair of track wheels installed beneath the housing 101 to provide movement to the housing 101. Herein, an artificial intelligence-based imaging unit 103 mounted on the housing 101 to capture multiple images in proximity to the housing 101 for analyzing pathways and monitoring obstacle while the housing 101 in movement.

[0025] The artificial intelligence based imaging unit 103 is constructed with a camera lens and a processor, wherein the camera lens is adapted to capture a series of images of the surrounding present in proximity to the housing 101. The processor carries out a sequence of image processing operations including pre-processing, feature extraction, and classification. The image captured by the imaging unit 103 is real-time images of the housing’s 101 surrounding. The artificial intelligence based imaging unit 103 in communication with a microcontroller, wherein the microcontroller used herein is an Arduino Uno microcontroller. The artificial intelligence based imaging unit 103 transmits the captured image signal in the form of digital bits to the microcontroller.

[0026] The microcontroller upon receiving the image signals compares the received image signal with the pre-fed data stored in a database and constantly determines pathways and obstacles while moving the housing 101 is in movement. Based on the determined obstacle and path, the microcontroller actuates the track wheels to move the housing 101 securely. The track wheels consist of rugged threads or cleats that provide traction and prevent slipping of the housing 101. The wheels are connected to an electric motor which propels the housing 101 forward or backward. This allows the housing 101 to move efficiently across surfaces like gravel, dirt, mud, or uneven terrain.

[0027] The device features a pair of clamps 104 installed inside the housing 101 with the help of four-bar linkage mechanism. Simultaneously, the microcontroller actuates a laser sensor installed in the housing 101, which is in sync with the imaging unit 103 to monitor organic samples. The laser sensor activates and emits a focused and narrow beam toward the surroundings. When the laser beam strikes the surroundings, it gets reflected back towards the sensor. The receiver of the primary laser sensor captures the reflected light and employs a time-of-flight measurement principle to determine the organic samples.

[0028] Based on the monitored sample, the microcontroller actuates the clamps 104 to grip the samples and put it in a box 102 inside the housing 101. The clamp includes a pair of flaps which are pivoted with each other for allowing the axial motion of the flaps required for gripping the samples, a DC motor is paired with the pivot joint that is activated by the microcontroller for providing a rotational motion to the joint for automating the movement of the flaps for gripping the samples.

[0029] A GPS (global positioning system) unit installed with the housing 101 records a location with respect to where the samples were collected. The GPS (Global Positioning System) unit consists of a receiver that communicates with the satellites to determine the exact location of the samples. The GPS (Global Positioning System) unit constantly receives signals from the satellites and calculates the coordinates. The GPS unit works by receiving signals from multiple satellites orbiting the Earth.

[0030] The GPS unit uses the timing of these signals and trilateration to calculate the precise location of the samples. The microcontroller linked with the GPS (Global Positioning System) unit processes the data received from the GPS (Global Positioning System) unit and transmits the sample’s precise location data. The real-time location is then sent to the microcontroller, which actuates an electrochemical biosensor installed in one of the clamps 104 to monitor microbial activity of material present on the sample.

[0031] The electrochemical biosensor detects microbial activity by measuring the electrical changes that occur when microorganisms in the sample metabolize or interact with a substrate. The process begins with sample preparation, where a sample is placed on the biosensor surface. Microorganisms present in the sample then grow and multiply on the biosensor surface, interacting with a substrate that is immobilized on the surface. This interaction triggers an electrochemical reaction, generating an electrical signal that is detected by the biosensor and converted into a measurable response. The magnitude of this response is directly related to the level of microbial activity present in the sample. Electrochemical biosensors detect various microbial activities, including metabolic activity, enzyme activity, microbial growth, and antibiotic resistance.

[0032] After detecting activity of material present on the sample, the microcontroller actuates a chamber 106 located within the housing 101 and houses an articulate telescopic gripper 107 and dual-axis lead screw mechanism 108. The articulate telescopic gripper 107 is mounted on the dual-axis lead screw mechanism 108 and has a claw or jaws that open and close to grasp the sample. It moves in and out (telescopic motion) to pick up the sample from the box 102 and can rotate and move in x-y directions (articulate motion) to place the sample onto a conveyor belt 109.

[0033] The conveyor belt 109 is located within the housing 101 and transports the sample from the gripper to the container 110. The container 110 is located within the housing 101 and receives the sample from the conveyor belt 109 configured with a primary Peltier unit and water sprayer to maintain the temperature and moisture of the sample within a predetermined range. The primary Peltier unit is a thermoelectric that heats or cools the sample to maintain a precise temperature. The water sprayer sprays a controlled amount of water onto the sample to maintain a precise moisture level.

[0034] An articulated arm 111 disposed within the housing 101 and consists of a series of connected links or segments. This allows it to move in multiple directions, enabling it to reach into the container 110 and pick up the sample. The arm has a gripper or claw at the end, which is used to grasp and hold the sample. The arm then moves to place the sample onto an agar plate 112, which is provided within the housing 101. The agar plate 112 is a petri dish or similar container 110 filled with agar gel, used for culturing microorganisms or other biological samples.

[0035] An artificial intelligence-based camera 113 is installed in the housing 101 and for capturing images in the vicinity of the housing 101. The camera 113 captures images of the area and sends them to the processor for processing. The processor then uses artificial intelligence algorithms to analyze the images and determine the position of the slide held by the arm. The processor then sends a signal to the arm to move and place the slide against the flame of the burner.

[0036] An articulate L-shaped telescopic bar 114 has a swab 115 at the end and is actuated by the processor to smudge the sample on the slide. The bar 114 moves in an L-shape to reach the slide and smudge the sample. The burner is provided within the housing 101 and has a flame that changes color based on the type of bacteria present in the sample. The camera 113 continues to capture images and process them to determine the type of bacteria based on the change in color of the flame. The arm places the slide against the flame, and the burner's flame changes color based on the type of bacteria present. The camera 113 then captures an image of the changed flame color and processes it to determine the type of bacteria.

[0037] A tank 116 is configured with a primary nozzle 117 and is provided within the housing 101 to store a catalyst. The catalyst is dispensed onto the sample by the arm in a tray 124. The tank 116 has several components, including catalyst storage, a primary nozzle 117, and a pump. The catalyst storage stores the catalyst, the primary nozzle 117 dispenses it onto the sample, and the pump pumps the catalyst from the storage to the primary nozzle 117.

[0038] The internal working of the tank 116 is as follows. The tank 116 stores the catalyst, which is pumped to the primary nozzle 117 by the pump. The primary nozzle 117 then dispenses the catalyst onto the sample in the tray 124. The camera 113 detects bubbling in the sample, indicating the presence of oxygen release from microbes. The arm moves the sample in the tray 124 to the tank 116 to dispense the catalyst. The camera 113 detects bubbling in the sample and determines the presence of oxygen release from microbes.

[0039] A compartment 118 is configured with a secondary nozzle 119 and contains an enzyme that is dispensed onto the agar plate 112. The compartment 118 has several components, including enzyme storage, a secondary nozzle 119, and a pump. The enzyme storage stores the enzyme, the secondary nozzle 119 dispenses it onto the agar plate 112, and the pump pumps the enzyme from the storage to the secondary nozzle 119. The internal working of the compartment 118 is as follows. The compartment 118 stores the enzyme, which is pumped to the secondary nozzle 119 by the pump. The secondary nozzle 119 then dispenses the enzyme onto the agar plate 112. The camera 113 detects clear zones in the sample to determine starch hydrolysis. The camera 113 detects clear zones in the sample and determines starch hydrolysis.

[0040] The camera 113 captures images of the sample and sends them to the processor for processing. The processor analyzes the images to detect clear zones in the sample. The processor then determines starch hydrolysis based on the clear zones. This component uses a compartment 118 to store an enzyme, which is dispensed onto the agar plate 112 by the secondary nozzle 119. The camera 113 then detects clear zones in the sample, indicating starch hydrolysis.

[0041] An articulated L-shaped telescopic link 120 has a receptacle 121 at the end that contains LCB (Lactophenol Cotton Blue). The link 120 is designed to drip the LCB onto the tray 124 that holds the sample. The link 120 has several components, including LCB storage, a receptacle 121, a telescopic link 120, and an articulated joint. The LCB storage stores the LCB, which is then dripped onto the tray 124 through the receptacle 121. The telescopic link 120 extends and retracts to position the receptacle 121 over the tray 124, and the articulated joint allows the link 120 to move in multiple directions to reach the tray 124.

[0042] The camera 113 is used to identify the type of microbe based on spore structure and arrangement observed. The camera 113 captures images of the sample and sends them to the processor for processing. The processor analyzes the images to identify the type of microbe based on spore structure and arrangement observed, and then stores the results in memory.

[0043] A sectioned vessel 122 located within the housing 101, which is designed to store and preserve samples based on the type of microbe detected. The vessel 122 is divided into sections, each with a different chemical environment, allowing for the storage and preservation of various types of microbes. This means that the device is able to handle multiple types of microbes simultaneously, and store them in a way that maintains their viability and integrity.

[0044] Each section of the vessel 122 is specifically designed to meet the needs of a particular type of microbe. For example, some sections may have a nutrient-rich environment, while others may have a more neutral or acidic environment. This allows the device to support the growth and survival of a wide range of microbes, and ensures that they remain healthy and viable for further analysis.

[0045] A temperature sensor is embedded in the chamber 106 and detects the temperature of the sample and this information is then used to regulate the operation of the primary Peltier unit. The temperature sensor consists of a thermistor, microcontroller, and signal conditioning circuitry. The thermistor measures the temperature of the sample and sends a signal to the microcontroller, which processes the data and determines the temperature. The microcontroller then regulates the operation of the Peltier unit to maintain a consistent temperature.

[0046] The primary Peltier unit is responsible for maintaining a consistent temperature for the sample. It is regulated by the temperature sensor and comprises a thermoelectric material, heat sink, and fan. The thermoelectric material converts electrical energy into thermal energy, heating or cooling the sample as needed. The heat sink and fan work together to dissipate heat and maintain a consistent temperature.

[0047] A moisture sensor is embedded in the chamber 106 and detects the moisture level of the sample. This information is then used to regulate the operation of the water sprayer. The moisture sensor consists of a humidity sensor, microcontroller, and signal conditioning circuitry. The humidity sensor measures the relative humidity of the chamber 106 and sends a signal to the microcontroller, which processes the data and determines the moisture level of the sample.

[0048] Based on the moisture level reading, the microcontroller regulates the operation of the water sprayer to maintain a consistent moisture level. The water sprayer comprises a water reservoir, pump, and spray nozzle. The water reservoir stores water for spraying, the pump pressurizes the water, and the spray nozzle atomizes it into a fine mist for even distribution. When the microcontroller receives a signal to spray water, the pump pressurizes the water, and the spray nozzle atomizes it into a fine mist. The mist is evenly distributed over the sample to maintain a consistent moisture level.

[0049] A touch-enabled display unit 123 installed on the housing 101 to allow the user to initiate sample analysis and display results of analyses performed. The display unit 123 consists of a display screen. The user interacts with the display screen to initiate sample analysis, and the microcontroller processes the user input and sends a signal to the analysis unit. The analysis unit performs analysis on the sample and comprises a processor, memory, and analysis protocols. The processor executes analysis algorithms on the sample data, and the memory stores analysis algorithms and data. The analysis algorithms perform specific analyses on the sample data and generate results.

[0050] A vessel 122 is configured with secondary Peltier units to maintain the temperature of the vessel 122 based on the type of microbe stored. This means that the vessel 122 is equipped with additional temperature control units that can heat or cool the vessel 122 as needed to maintain the optimal temperature for the specific type of microbe being stored. The secondary Peltier units are strategically located within the vessel 122 to provide precise temperature control. These units are designed to work in conjunction with the primary Peltier unit to maintain a consistent temperature throughout the vessel 122. This ensures that the microbes are stored in optimal conditions, which is crucial for their growth and survival.

[0051] The present invention works best in following manner, where operation of the invention begins with collection of a sample using the clamps 104 and four-bar linkage mechanisms 105, and then stores it in a box 102 within the housing 101, recording the location from where the sample was collected using its GPS unit. Next, the articulated telescopic gripper 107 picks up the sample and places it on a conveyor belt 109, which transports it to a container 110 within the housing 101. The electrochemical biosensor then detects microbial activity in the sample. The primary Peltier unit and water sprayer maintain optimal temperature and moisture levels for the sample. The articulated arm 111 then places the sample on an agar plate 112, and the burner flame changes color based on the type of bacteria present. The tank 116 with a primary nozzle 117 dispenses a catalyst onto the sample in a tray 124, and the camera 113 detects bubbling in the sample to determine oxygen release from microbes. The compartment 118 with a secondary nozzle 119 dispenses an enzyme onto the agar plate 112, and the camera 113 detects clear zones to determine starch hydrolysis. The articulated L-shaped telescopic link 120 then drips LCB onto the tray 124 with the sample, and the camera 113 identifies the type of microbe based on spore structure and arrangement. Finally, the sectioned vessel 122 stores and preserves the samples based on the type of microbe detected, and the touch-enabled display unit 123 shows the results of the analyses performed.

[0052] Although the field of the invention has been described herein with limited reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the invention, will become apparent to persons skilled in the art upon reference to the description of the invention. , Claims:1) A microbe collection and analysis device, comprising:

i) a cuboidal housing 101 having a pair of tracked wheels underneath said housing 101 for a locomotion of said housing 101;
ii) an artificial intelligence-based imaging unit 103, installed on said housing 101 and integrated with a processor for recording and processing images in a vicinity of said housing 101, to determine pathways and obstacles during locomotion of said housing 101 to accordingly actuate said tracked wheels to navigate said housing 101 without collisions with obstacles;
iii) a pair of clamps 104 mounted with said housing 101 by means of four-bar linkage mechanisms 105, wherein said imaging unit 103 in synchronisation with a laser sensor embedded in said housing 101, to determine organic samples to actuate said linkage and said clamps 104 to fetch said samples and store in a box 102 within said housing 101, wherein a GPS (global positioning system) unit incorporated on said housing 101 records a location from where said samples were collected;
iv) an electrochemical biosensor embedded in one of said clamps 104, to detect microbial activity of material present on said sample;
v) a chamber 106 disposed within said housing 101, wherein an articulate telescopic gripper 107 mounted on a dual axis lead screw mechanism 108 located within said housing 101 for picking said sample from said box 102 to place onto a conveyor belt 109 to convey said sample into a container 110 within said housing 101, wherein said container 110 is configured with a primary Peltier unit and a water sprayer for maintaining temperature and moisture of said sample within a predetermined temperature and moisture range;
vi) an articulated arm 111 disposed within said housing 101 for picking said sample from said container 110 and placing onto an agar plate 112 provided within said housing 101;
vii) an artificial intelligence-based camera 113, installed in said housing 101 and integrated with a processor for recording and processing images in a vicinity of said housing 101, to determine position of a slide held by said arm, to actuate an articulate L-shaped telescopic bar 114 having a swab 115 at an end, to smudge said sample on said slide, wherein said arm places said slide against a flame of a burner provided within said housing 101 to establish type of bacteria based on change in colour of said flame;
viii) a tank 116, configured with a primary nozzle 117, provided within said housing 101 to store a catalyst to dispense on said sample by said arm in a tray 124, wherein said camera 113 detects bubbling in said sample, to determine presence of oxygen release from microbes;
ix) a compartment 118, configured with a secondary nozzle 119, containing enzyme to be dispensed on said agar plate 112, wherein said camera 113 detects clear zones in said sample to determined starch hydrolysis; and
x) an articulated L-shaped telescopic link 120, having a receptacle 121 at an end, containing LCB (lactophenol cotton blue) to drip said LCB onto tray 124 having sample, wherein said camera 113 identifies type of microbe based on spore structure and arrangement observed.

2) The device as claimed in claim 1, wherein a sectioned vessel 122 located within said housing 101, having differing chemical environments, for storage and preservation of samples based on type of microbe detected.

3) The device as claimed in claim 1, wherein a temperature sensor is embedded in said chamber 106 to detect temperature of said sample to regulate operation of said primary Peltier unit.

4) The device as claimed in claim 1, wherein a moisture sensor is embedded in said chamber 106 to detect moisture level of said sample to regulate operation of said water sprayer.

5) The device as claimed in claim 1, wherein a touch-enabled display unit 123 disposed on said housing 101 to enable user to initiate sample analysis and display results of analyses performed.

6) The device as claimed in claim 1, wherein said vessel 122 is configured with secondary Peltier units to maintain temperature of said vessel 122 based on type of microbe stored.