Description:CROSS-REFERENCE TO RELATED APPLICATIONS AND PRIORITY
The invention comprises an improvement in or modification of the invention claimed in the specification of the Indian patent application number 202221053149, applied for, on 16 September 2022.
TECHNICAL FIELD
The present disclosure relates to a compact diagnosis system. Particularly, the present subject matter relates to a portable and integrated diagnosis system enabling extraction and PCR testing. More particularly, the present invention provides a system that automates a series of steps beginning with biomolecule extraction and PCR testing.
BACKGROUND
Polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used in molecular biology. Owing to limitations such as large size, high power consumption, and complicated operation, PCR is only used in hospitals or research institutions. Nucleic Acid (NA) extraction is used in many types of biological research including molecular biology, forensics, pathology, environmental research, and drug discovery.
Nowadays, the diagnostic healthcare system suffers majorly because of increasing pressure and lack of adequate laboratories and infrastructure. Further, laboratories require different kinds of equipment to perform the tests which is expensive and heavy weight. Further, available technology of diagnostic machinery is for limited population leading to large-scale exclusion of poor patients from affordable health care. Particularly focusing on PCR (Polymerase Chain Reaction) testing equipment we find that most of these systems are large and heavy weight leading to constraints on transportation.
Further, this equipment is primarily designed for large capacity and large sample size making it unaffordable for smaller size testing. In remote geographical areas like hilly or areas where the population is sparse and overall health infrastructure is minimal such large systems are not available leading to the exclusion to masses from utilizing the healthcare provisions.
Features to look for in choosing an automated DNA extraction system include integration with laboratory information management systems, full start-to-finish automation, error control, and safeguards against contamination. Therefore, a diagnosis system that is portable, compact, and lightweight equipped to conduct nucleic acid extraction and PCR testing thus providing highly reliable inputs to health care professionals at low cost and reduced time is highly desirable is the need of the hour. Further, such types of diagnostics systems are also high in maintenance, and there felt a need to develop a diagnostic system that is low in maintenance and mechanically stable.
Thus, the present invention addresses the above long-felt need. It discloses a portable and integrated diagnosis system, which is compact and lightweight with optimized cycle time. The proposed system provides novel modifications or improvements in the diagnostic system by way of having in-expensive manufacturing, affordable maintenance or maintenance-free, and scalable along with ease of operational aspect.
Object of the Invention
An object of the present disclosure is to provide a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner.
It is another object of the present disclosure is to produce a high precision, rapid throughput unit at a cost lower than previously attainable.
It is another object of the present disclosure to provide a system that is easily portable, compact, and table-top mounted.
It is another object of the present invention to provide a system having a tip holder assembly to perform a series of steps from extraction to PCR in an automated manner without any human intervention.
One more object of the present invention is to provide a system that is mechanically stable, accurate, maintenance-free, and scalable.
SUMMARY
The present invention provides a portable and integrated diagnosis system enabling extraction and PCR testing. The entire design of this system is devoted to producing a high precision, rapid throughput unit at a cost lower than previously attainable. The system as designed is mechanically stable, accurate, maintenance-free, and scalable. This section is not intended to identify essential features of the claimed subject matter, nor it is intended to be used in determining or limiting the scope of the claimed subject matter.
The words angular variable rotor or shaft are alternatively used and bear the same meaning throughout the specification. In an aspect, the present invention provides a portable diagnosis system with integrated PCR and extraction. The portable and integrated diagnosis system may comprise an extraction assembly for the extraction of nucleic acid mounted over it along with an integrated real-time PCR assembly. The portable and integrated diagnosis system may further comprise an extraction assembly comprising a deck assembly and a tip holder assembly.
Further, the PCR assembly the deck assembly, and the tip holder assembly are functionally coupled with each other to reduce cycle time for each step involved in extraction and PCR.
In an aspect, the present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of :
a housing having a base plate (100a) top loaded with a deck plate assembly (200), wherein the deck plate assembly comprises a movable deck (200a), a movable collection tray (201), and a lifting holder (202),
a tip holder assembly (300) comprises one or more tips (301), a pair of tips with a notch (301a-302b), a picker plate (302), a shock-absorbing spring (303), a linear bearing (304), an ejector housing and a picker pin (305);
a thermocycler with a cover (400), wherein the cover comprises a pair of springs (401), a set of mounting pins (402), a set of Location pins (403), a set of location magnets (404), and a pickup edge (405) for lifting the cover;
a thermoshaker assembly (500), wherein the assembly comprises a tube holder (501) in a linear arrangement, an insulating material plate (502) placed in between a heater plate (503) and a motor plate (504), a location rubber (505), a linear bearing (507), and a motor (506);
a centrifuge (600) with angular variable rotors (601), a tilting mechanism (603), and a rectangular sample holder (601-1, 601-2, 601-3, 601-4), and an open window flap cover (606).

In another aspect present invention provides a portable and integrated diagnosis system, wherein a movable deck (200) and movable collection tray (201) are movable and autoclavable.
In yet another aspect present invention provides a portable and integrated diagnosis system, wherein the pair of tips of tip holder (300) assembly with the notches (301a, 301b) of the tip holder assembly (300) lifts the cover (400) of the thermocycler by lifting the pickup edges (405) with the notch (301a, 301b) of the tips.
In another aspect present invention provides a portable and integrated diagnosis system, wherein an insulating material is selected from Teflon, glass, plastic, rubber, foam, cellulose, and wool.
In yet another aspect present invention provides a portable and integrated diagnosis system, wherein the tilting mechanism (603) of the centrifuge (600) provides an angle of rotation to angular variable rotors (602), wherein the angle may vary from 0 to 50 degrees.
In yet another aspect present invention provides a portable and integrated diagnosis system, wherein the tilting mechanism (603) of the centrifuge (600) provides an angle of rotation to angular variable rotors (602), wherein the angle may vary from 0 to 45 degrees.
In another aspect present invention provides a portable and integrated diagnosis system, wherein the centrifuge (600) provides spinning rotation ranging from 500 to 2700 RPM.
In another aspect present invention provides a portable and integrated diagnosis system, wherein the centrifuge (600) provides spinning rotation ranging from 600 to 2000 RPM.
In another aspect present invention provides a portable and integrated diagnosis system, wherein the deck assembly (200), tip holder assembly (300), thermocycler (400), thermoshaker (500), and centrifuge (600) are each top mounted separately over the base plate (100a).
BRIEF DESCRIPTION OF DRAWINGS
The present invention is described with reference to the accompanying figures. In the Figures, the left-most digit(s) of a reference number identifies the Figure in which the reference number first appears. The same numbers are used throughout the drawings to refer like features and components.
The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which are:
Figure 1 illustrates top mounted view of integrated system
Figure 2 illustrates bottom view of integrated system
Figure 3 illustrates top view of movable deck plate assembly
Figure 4a illustrates back view of tip holder
Figure 4b illustrates side view of the tip holder
Figure 4c illustrates front view of the tip holder
Figure 5a illustrates front side view of thermocycler cover
Figure 5b illustrates a side view of the thermocycler cover
Figure 5C illustrates back side view of the thermocycler cover
Figure 5D illustrates a back side view of the thermoshaker
Figure 6a illustrates a side view of the thermoshaker
Figure 6b illustrates linear arrangement of the thermoshaker unit
Figure 7a illustrates the top view of the centrifuge
Figure 7b illustrates a bottom view of the centrifuge
Figure 7c illustrates tilted angle view of a sample holder
Figure 7d illustrates the open and closed position view of a sample holder

DETAILED DESCRIPTION
Reference throughout the specification to “various embodiments,” “some embodiments,” “one embodiment,” or “an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, appearances of the phrases “in various embodiments,” “in some embodiments,” “in one embodiment,” or “in an embodiment” in places throughout the specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures or characteristics may be combined in any suitable manner in one or more embodiments.
In accordance with the present invention, a compact and portable system for extraction integrated with a PCR is disclosed. The system may comprise a plurality of assemblies provided for performing extraction and PCR.
In an embodiment of the present invention the assemblies of the system such as deck plate assembly, tip holder assembly, thermocycler, thermoshaker assembly, and centrifuge assembly are top mounted over the base of the system. The assemblies are top-mounted in such a way that each one of the assemblies can be individually removable without any disturbance to the other assemblies and thus this helps to avoid opening all the components of the system together for malfunction of a single assembly. Thus, each of these assembles is provided with an individual frame structure for ease of operation and maintenance purposes.
In an embodiment present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base structure top loaded with a deck plate assembly, wherein the deck plate assembly comprises a movable deck, movable collection tray, and lifting holder, wherein the lifting holder lifts the movable deck by manual force. The deck plate assembly comprises consumables for carrying out PCR. The deck of assembly is movable and can be interchangeable with a deck plate having a different arrangement of consumables based on user requirements. The movable deck plate can be manually removable by lifting a lifting holder present on both sides of the deck plate. Thus, the movable deck plate can be removed from the assembly for cleaning and autoclaving.
In an embodiment present invention uses a motion control card used for robotic arm kinematics with a communication speed of 100mbps
In an embodiment present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base structure top loaded with a tip holder assembly comprising one or more tips, a pair of tips with a notch, a picker plate, a shock-absorbing spring, a linear bearing, ejector housing, and a picker pin, wherein the linear bearing in a vertical slide aids a parallel movement of both end of connected plate at a time. More particularly the tip holder assembly comprises at least four tips. The tips of the tip holder assembly are further comprised of a notch on one side of the first tip and the opposite side of the last tip, such that two notches are in opposite directions to each other, to aid in lifting the cover of PCR assembly to open/close and to aid in sliding the cover of centrifuge assembly for opening and closing. Furthermore, the tips of the tip holder assembly have a picker plate, shock-absorbing spring, ejector housing, and picker pin. Wherein, the picker plate, motor support sliding plate, ejector housing, and linear shaft are made up of self-lubricating material which aids in ease of movement of the tips. The use of self-lubricating material avoids frequent oiling of machine parts and hence remains self-maintained for a long-lasting period and provides mechanical stability with enhanced operation efficiency. Further, the spring installed in between the picker plate and Picker pin helps to avoid excess force applied on consumables to absorb shocks during operation. Also, the use of a shock-absorbing spring helps with self-alignment of the end of tips during pipetting operation to avoid damage to the consumables. The tip holder assembly further discloses the use of linear bearing in vertical slides for parallel both ends of picker's plate at a time, which helps to avoid misalignment of tips. The use of a linear bearing aids the mechanical stability of the tip holder assembly.
In an embodiment, self-lubricating material as disclosed in the present invention is gunmetal such as brass.
In an embodiment of the present invention movement of the tip holder assembly is supported by a robotic clamp, wherein the clamp is freely movable in all possible directions depending on the protocols to be set for carrying out RT-PCR.
In an embodiment present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base structure top loaded with thermocycler having a cover, wherein the cover comprises a pair of springs for auto alignment, a set of mounting pins, a set of Location pins, a set of location magnets, and a pickup location for lifting the cover, wherein the location magnet provides a seal-tight closing to the thermocycler to maintain temperature during PCR cycle. The location magnet exerts a magnetic field on the cover which ensures the seal-tight closing of the thermocycler.
In an embodiment, the notches present on the end of tips help to lift the pickup edges of the thermocycler cover for opening and closing the thermocycler assembly. The tip holder assembly lifts the cover of the thermocycler assembly and then picks up and places the sample tube for a PCR run. After the successful transfer of PCR tubes in the thermocycler, the tip holder assembly again lifts the cover and places it back on the thermocycler. The use of a tip holder assembly in lifting of thermocycler door avoids the use of a battery for opening and closing of thermocycler and hence these aids in the low-cost manufacturing of the system. Further, such type of mechanism provides mechanical stability and low maintenance of thermocycler assembly.
In an embodiment present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base structure top loaded with a thermoshaker assembly, wherein the assembly comprises a tube holder in a linear arrangement, an insulating material plate placed in between the heater plate and motor plate, location rubber, linear bearing, and motor. The thermoshaker assembly with linear sample tube holder arrangement is located behind the deck plate assembly, which helps to prevent cross-contamination during the placing of sample tubes in the tube holder of the thermoshaker assembly. Furthermore, each sample tube holder of the thermoshaker assembly is positioned parallel to each set of consumables on the deck plate respectively, which helps the tip holder assembly to pick up the sample tube from the deck plate and drop it into the respective tube holder of thermoshaker which is aligned exactly at the backside of the deck plate, and thus this helps to avoid cross-contamination of sample along with error free pick and place of the sample tube holder.
In an embodiment, the insulating material plate placed in between the heater plate and motor plate is made of material selected from Teflon, glass, plastic, rubber, foam, cellulose, and wool.
In a particular embodiment, the insulating material used is Teflon. Thus, the use of a Teflon plate as an insulating material act as a barrier to transfer heat to the motor plate and the other electrical parts and thus avoids unnecessary heating issues of same and this helps the heater to generate only the desired quantity of heat required for operation. Thus, as the heat fails to transfer to the motor plate and other electrical parts, it prevents damage to the motor and other electrical parts and hence increases the life of the motor and other electrical parts. Furthermore, heating efficiency has been increased due to the Teflon plate, and the time required to attain the desired temperature has been reduced from 7 -10 minutes to 1- 2 minutes.
In an embodiment present invention provides a portable and integrated diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base structure top loaded with a centrifuge with angular variable rotors, a tilting mechanism, and a rectangular sample holder, and a open window flap cover, wherein the tilting mechanism provides the variable angle of rotation to centrifuge. The centrifuge has angular variable rotors which provide the desired angle of rotation for centrifugation. The centrifuge has a tilting mechanism, wherein the timer pully in coordination with the link plate provides the desired angle to the sample tube to carry out centrifugation. The tilting mechanism uses a tapper lock mechanism for the alignment of the position of the sample tube holder. The desired angle may vary from 0 to 50 degrees. Furthermore, the centrifuge assembly could also rotate at a lower speed. This type of centrifuge with an angular variable rotor could be able to provide the speed of rotation ranging from 600 to 2500 RPM. Furthermore, the rotor is also made up of gunmetals.
In an embodiment, the centrifuge assembly possesses a cover having a single rectangular-shaped window. The window has a slidable cover with a rod, wherein the rod is located at the middle of the window cover. The tip holder assembly pushes from the front side of the rod toward the opening position, and after placing of sample for centrifugation it again pushes the rod from the rear side of the rod towards the closing position, which covers the centrifuge assembly with a window cover. Thus, as the cover uses only mechanical force to open and close the window cover, it eliminates the need for a battery and hence provides long-term stability, hence this also resulted in a low-cost manufacturing and maintenance system.
The shape of the sample holder may be selected from but not limited to rectangular, circular, or square, and accordingly the shape of the window of cover changes.
In an embodiment, the centrifugation is carried out at a 45-degree angle, the achieved angle enhances the surface area in sample processing and hence achieves better extraction processing. Now referring to Figure 1, a portable top-mounted view of diagnosis system 100 enabling extraction with integrated PCR is disclosed. The portable and integrated diagnosis system 100 may comprise a base structure 101a. The assemblies of the system such as deck plate assembly (200), tip holder assembly (300), thermocycler (400), thermoshaker assembly (500), and centrifuge assembly (600) are top-mounted over the base (101a) of the system.
Now referring to Figure 2, the collection tray (201) is located at the bottom of the deck plate. When there is any excess spillage of liquid during pipetting operation the excess liquid accumulates in the collection tray (201). The collection tray itself can be removable for cleaning, which aids in avoiding cross-contamination. The thermoshaker assembly (500) is located exactly behind the deck plate assembly (200) for ease of operation. The PCR assembly (401) is located beside the centrifuge assembly.
Now referring to Figure 3, illustrates deck plate (200a) securely holds cartridges, tubes, piercing tips, and tip pipettes from moving relative to the deck assembly during the extraction process. Further, the placement of the cartridges, the tubes, the tips, and tip pipettes in the plurality of receptacles is such that, it enables a reduction in extraction cycle time. The reduction in cycle time is further due to the reduction in movement of the tip holder assembly to access the cartridges, tubes, tips, and pipettes while performing a task. The deck plate assembly comprises a movable deck (200), a movable collection tray (201), and a pair of lifting holders (202), wherein the lifting holders (202) lift the movable deck (200) by manual force. The deck plate (200a) comprises consumables for carrying out PCR. The deck (200a) of assembly is movable and can be interchangeable with a deck plate having a different arrangement of consumables based on user requirements. The movable deck plate (200a) can be manually removable by lifting a lifting holder (202) present on both sides of the deck plate (200a). Thus, the movable deck plate can be removed from the assembly for cleaning and autoclaving. The collection tray (201) is located at the bottom of the deck plate. When there is any excess spillage of liquid during pipetting operation the excess liquid accumulates in the collection tray (201) and as the collection tray is also movable it can be easily removed for cleaning.
Referring to Figures 4a, 4b, and 4c, illustrate tip holder assembly (300) configured to freely move in all possible 360-degree angles, wherein the movement of the tip holder is supported by a robotic arm structure. The robotic arm can move freely like a wrist movement of a hand depending on the test protocols. Wherein the robotic arm has a free degree of movement in all possible directions. The robotic arm as disclosed in the present invention is like a finger clamp, wherein the finger clamp can move freely in all possible directions to perform automated steps of extraction followed by PCR. The tip holder assembly comprising one or more tips (301), a pair of tips with a notch (301a, 301b), a picker plate (302), a shock-absorbing spring (303), a linear bearing (304), ejector housing (308), and a picker plate (302), wherein the linear bearing (304) in a vertical slide aids a parallel movement of picker plate of both end at a time. More particularly the tip holder assembly comprises at least four tips. The tips (302) of the tip holder assembly (300) are further comprised of a notch on one side of the first tip (301b) and the opposite side of the last tip (301a), such that two notches are in opposite directions to each other, to aid in lifting the cover (406) of PCR assembly (400) to open/close and to aid in sliding the cover (609) of centrifuge assembly (600) for opening and closing. The spring (303) is installed in between the picker plate (302) and the Picker pin helps to avoid excess force applied on consumables to absorb shocks during operation. Also, using a shock-absorbing spring (303) helps with the self-alignment of the end of tips (305) during pipetting operation to avoid damage to the consumables. The tip holder assembly (300) further discloses the use of linear-bearing (304) in vertical slides for parallel movement of both picker's plates at both ends at the same time, which helps to avoid misalignment of tips. Using a linear bearing (304) aids the mechanical stability of the tip holder assembly (300). Figure 4b illustrates motor LR (307) for the movement of the tip holder assembly and ejector motor (308) for the ejection of picker pins. The linear bearing (304) helps in the self-alignment of both ends of the pickers plates for adjusting the distance between the tips of the tip holder assembly.
Now referring to Figures 5a, 5b, and 5c illustrate the front, side, and back side overview of the thermocycler assembly with cover (400). thermocycler having a cover (400), wherein the cover comprises a pair of springs (401) for auto alignment, a set of mounting pins (402), a set of Location pins (403), a set of location magnets (404), and a pickup edge (405) for lifting the cover, wherein the location magnet (404) provides a seal-tight closing to the thermocycler to maintain temperature during PCR run. The location magnet exerts a magnetic field on the cover which ensures the seal-tight closing of the thermocycler. The pointed portion of the location pins (403) fits into the hole (406), which avoids any further movement of cover and ensures proper and seal-tight closing of the thermocycler.
Now referring to figures 6a, 6b, and 6c illustrate different views of thermoshaker assembly (500). The assembly comprises a tube holder (501) in a linear arrangement, an insulating material plate (502) placed in between the heater plate (503) and motor plate (504), location rubber (505), linear bearing (507), and motor (506).
Figures 7a, 7b, 7c, 7d, and 7e illustrate centrifuge assembly (600). The centrifuge assembly comprises angular variable rotors (602), a tilting mechanism (603), a rectangular sample holder (601), and an open window flap cover (606). The tilting mechanism (603) provides the variable angle of rotation to the centrifuge. The centrifuge has a tilting mechanism (603), wherein the pully (604) in coordination with the link plate (605) provides the desired angle to the sample tube to carry out centrifugation. The centrifuge assembly possesses a cover (606) having a single rectangular-shaped window (607). The window has a slidable cover (609) with a rod (608), wherein the rod is located in the middle of the window cover (606). The tip holder assembly pushes from the front side of the rod (608) toward the opening position (607b), and after placing of sample for centrifugation it again pushes the rod from the rear side of the rod (608) towards the closing position (607a), which covers the centrifuge assembly with a window cover (609).
In an embodiment of the present invention tip holder assembly (300) opens the centrifuge by pushing the rod (608), to aid in sliding of window cover (609) in an open position (607b). In the next step tip holder assembly (300) picks up the sample tube from deck assembly (200) and travels towards the centrifuge (600) to load samples in the sample tube holder (601-1, after loading rotors of the centrifuge rotate to move loaded sample holder (601-1) to the inner side, that automatically takes sample holder (602-2) at the window (607) position for further loading of sample tube in sample tube holder (602-2). Thus, sample tube holders (601-1,2,3,4) one after another in a sequence come at the window (607) position to load sample tubes for centrifugation. After the loading of the tip holder assembly push the back rod (608) at the closing position (607a) to cover the centrifuge assembly (600).
In an embodiment of the present invention angular movement of the sample tube holder (601) can be changed or programmable which increases the surface exposure by 40% for better operation at a minimum speed of 500 RPM.
In an embodiment of the present invention angular movement of the sample tube holder (601) can be changed or programmable which increases surface exposure by 40% for better operation at a speed of 600 RPM.
In another embodiment of the present invention design of the centrifuge is designed in such a way that it provides mechanical and electrical locks during rest position. Furthermore, in case of excessive load applied on the sample tube holder (601-1,2,3,4), it will not damage the module due to its mechanical resting flange design.
In yet another embodiment of the present invention wherein the tapper lock is used for timer pulley mounting to avoid the machining process
In yet another embodiment, the gunmetal is used in the primary and secondary shafts for free movement and self-lubrication.
The below-listed advantages of the prevent invention should be used for exemplary limitations and should not limit the scope of the prevent invention.
• The system is mechanically stable
• The system has electrical safety and low power consumption.
• Minimum use of batteries
• Easy for maintenance
• The system for portable and integrated diagnosis is easy to use and cost-effective
• The system is compact, fully automated, and thus easy to transport
• The system reduces time intervals between diagnoses/analyses
• The system optimizes the health care system in times of scarcity of resources and manpower
• The system provides a cost-effective solution for diagnosis requirements for areas with limited health infrastructure
• The system eliminates bigger infrastructure requirements
• The system is a sample to result in an application
• Software-driven system eliminates human intervention
• The multiple systems can be networked together to achieve maximum throughput
• The enclosed system prevents contamination-free sample processing
Various modifications to the embodiment will be readily apparent to those skilled in the art and the generic principles herein may be applied to other embodiments. However, one of ordinary skill in the art will readily recognize that the present disclosure is not intended to be limited to the embodiments illustrated but is to be accorded the widest scope consistent with the principles and features described herein.
The foregoing description shall be interpreted as illustrative and not in any limiting sense. A person of ordinary skill in the art would understand that certain modifications could come within the scope of this disclosure.
The embodiments, examples and alternatives of the preceding paragraphs or the description and drawings, including any of their various aspects or respective individual features, may be taken independently or in any combination. Features described in connection with one embodiment are applicable to all embodiments, unless such features are incompatible.

, Claims:We Claim:
1. A portable diagnosis system enabling integrated PCR and extraction in an automated manner, comprising of a housing having a base plate (100a) top loaded with:
a) a deck plate assembly (200), wherein the deck plate assembly comprises a movable deck (200a), a movable collection tray (201) with a lifting holder (202), to lift the movable deck (200a);
b) a tip holder assembly (300) comprises a pair of tips (301) with a notch (301a-302b), a picker plate (302), a shock-absorbing spring (303), a linear bearing (304), an ejector housing and a picker pin (305), wherein the linear bearing (304) in a vertical slide aids a parallel movement of the picker plates (302) of both ends at a same time;
c) a thermocycler with a cover (400), wherein the cover comprises a pair of springs (401), a set of mounting pins (402), a set of location pin (403), a set of location magnet (404), a pickup edge (405) for lifting the cover, wherein the location magnet (404) provides a seal-tight closing of thermocycler with cover to maintain a temperature during a PCR cycle;
d) a thermoshaker assembly (500), wherein the assembly comprises a tube holder (501) in a linear arrangement, an insulating material plate (502) placed in between a heater plate (503) and a motor plate (504), a location rubber (505), a linear bearing (507), and a motor (506); and
e) a centrifuge (600) with angular variable rotors (601), a tilting mechanism (603), a rectangular sample holder (601-1, 601-2, 601-3, 601-4), and an open window flap cover (606), wherein the tilting mechanism (603) provides the variable angle of rotation for centrifugation.
2) The portable diagnosis system as claimed in claim 1, wherein a movable deck (200) and movable collection tray (201) are movable and autoclavable.
3) The portable diagnosis system, as claimed in claim 1, wherein the pair of tip holder (300) assembly with the notches (301a, 301b) lifts the cover (400) of the thermocycler by lifting the pickup edges (405) with the notch (301a, 301b) of the tips.
4) The portable diagnosis system, as claimed in claim 1, wherein an insulating plate (502) is selected from material made up of teflon, glass, plastic, rubber, foam, cellulose, and wool, or a combination thereof.
5) The portable diagnosis system as claimed in claim 1, wherein the tilting mechanism (603) of the centrifuge (600) provides an angle of rotation to angular variable rotors (602), wherein the angle varies from 0 to 50degrees.
6) The portable diagnosis system as claimed in claim 1, wherein the centrifuge (600) provides spinning speed not limiting to a range from 600 to 2500 RPM.
7) The system as claimed in claim 1, wherein the deck assembly (200), tip holder assembly (300), thermocycler (400), thermoshaker (500), and centrifuge (600) are each separately top mounted over the base plate (100a).
Dated this 8th Day of March 2024