Description:TECHNICAL FIELD:
The present disclosure relates to a cosmetic application. More specifically, the present disclosure relates to a cosmetic composition comprising an unsymmetrically substituted quaternary ammonium compound/s as cationic surfactant co-acting with any one or more ingredients selected from the group consisting of i) any one or more of natural polymers and their derivatives, ii) any one or more of non-ionic or cationic surfactant/s with emulsifying properties, iii) any one or more of fatty alcohol/s having 8 to18 carbons or combination thereof. Further, the present disclosure relates to a cosmetic composition, which provides significant improvement in hair conditioning when used in hard water. Also, the cosmetic composition of the present disclosure does not affect the performance of any hair care treatments such as shampoo wash, washing hair with a two-in-one shampoo with an inbuilt conditioner, conditioner application, or hair straightening or strengthening or any hair care application involving a step of hair conditioning.

BACKGROUND:
Conditioners play a pivotal role in modern treatments including hair care routines, serving as essential products that contribute to the overall health, appearance, and manageability of hair. The present study focuses on hair treatment for illustrative purposes though the invention has broader application such as in skin care.

A high-quality hair conditioner contains a harmonious blend of key ingredients, including natural oils, humectants, proteins, and vitamins, along with certain botanical extracts. When applied after shampooing, a good conditioner works to restore moisture balance, smoothing the hair cuticle and reducing friction between strands. This result in improved detangling, reduced breakage, and enhanced softness. Moreover, conditioners contribute to the overall aesthetic appeal of hair. They improve hair's lustre, smoothness, and shine by sealing the cuticle, allowing light to reflect more evenly off the hair surface. This creates a visually striking and healthier appearance. It's important to note that not all conditioners are the same. There are various types of conditioners available, including rinse-out conditioners, leave-in conditioners, deep conditioners, and more, each designed to address specific hair care needs. The delivery of conditioning was tested using an expert panel guided by a protocol.

The expected delivery of these hair conditioning compositions along with other specific hair care options, depends on the nature of water used for rinsing the hair. It is well known that soft water provides hassle free hair care regime. Similarly, hardness in water results in inadequate cleaning of garments using detergents, unclean sticky feels on skin or hair after washing with surfactant-based products. However, access to softwater is a luxury seldom available for general public. Increase in total hardness of groundwater is noticed in large areas in several states of India. This is especially true when one is forced to use ground water instead of publicly supplied treated water such as municipal water. Thus, it is a challenge to have a product that works with all types of water – more particularly in hard water.

The deleterious effect of hardness on hair washing has been known in the art for a long time. The total oil/dart removal decreases with increasing water hardness, while the interfacial tension increases.

Tanthakit, P. etal., [Microemulsion formation and detergency with oily soil: V. Effects of Water Hardness and Builder. J Surfact Deterg, 12: 173-183(2009); https://doi.org/10.1007/s11743-009-1112-z] reported that, when hard water is used in laundering, the total oil removal improved with increasing concentrations of sodium tripolyphosphate (STPP) or Ethylenediamine tetraacetic acid (EDTA) up to stoichiometric levels, with STPP being slightly more effective than EDTA on a molar basis. Even high builder [materials that perform both functions: (1) increasing the removal of oil/dart and (2) preventing or minimizing its redeposition] concentrations could not improve hard water detergency to that of soft water. A significant fraction of oil/dart removal occurred in the rinse steps vs. the wash step. Increasing water hardness reduced this fractional oil/dart removal in the rinse steps, but it was still over half of the total oil removal at 1,000 ppm water hardness.

US20060286058A1discloses a system for cleaning and conditioning hair, in particular, fine, thin hair. The system comprises, as individually packaged compositions: (A) an aqueous pre-shampoo conditioner comprising: i) a cationic conditioning polymer having a charge density of at least 1.8 meq/g, ii) cationic conditioning surfactant, iii) a fatty alcohol material; and (B) an aqueous shampoo comprising: i) an anionic surfactant, and ii) a cationic conditioning agent selected from the group consisting of quaternary silicone polymers, quaternary polysaccharide polymers, quaternary cellulose derivatives, quaternary starch derivatives, quaternary guar gum derivatives and mixtures thereof. Methods for cleaning and conditioning hair using such aqueous pre-shampoo conditioners and shampoos are also disclosed. This reported composition is significantly different from the one described in the present invention by the way that, cationic conditioning polymer and anionic surfactant were not the part of the present system.

JP5000928Adiscloses a shampoo composition low in irritation to the skin and hairs, showing high foaming properties and high detergency, excellent in hard water resistance and having an excellent conditioning effect. The shampoo composition containing (A) a betaine-type compound of the formula-I e.g., N-dodecyl-N-(3- trimethylammonio-2-hydroxypropyl) aminoacetate and (B) a water-soluble cationic polymer, e.g. a cationized cellulose derivative, a cationic starch or a diallyl quaternary ammonium salt/acrylamide copolymer. The compounding ratio of the component (A) is preferably 0.1-50wt.% in the case of a liquid shampoo, 0.1-80wt.% in the case of a paste shampoo and 50-90wt.% in the case of a solid or powdery shampoo. The present invention does not use such ingredient more particularly betaine-type compounds. N-dodecyl-N-(3- trimethylammonio-2-hydroxypropyl) aminoacetate and similar molecules.

CN111317674A discloses a hair conditioning composition and a preparation method thereof. The first aspect of the present invention provides a hair conditioning composition comprising three phases. A phase: 1 to 5% of the weight of the composition of a cationic surfactant, the composition weight 1 to 5% of fatty alcohol and its derivative or alcohol ether and 1 to 5% of the weight of the composition of the grease, wherein cationic surfactants include alkyl quaternary ammonium salts, and alkyl carbon chain length of C8 to C22, fatty alcohols and derivatives thereof carbon atom number is 8~22, B phase: the adding amount of pure water, supplemented to be 100 % by weight of the composition; and/or C phase: the composition weight 1 to 5% of silicone oil, by weight of the composition, 0.5 to 3.5% antiseptic and essence of 0.5~2.5 of the weight of the composition. This reported composition is significantly different from the one described in the present invention by the way that, alcohol ether, silicone oil and antiseptics are not part of the present invention.

EP4037652A1 discloses a hair cosmetic compositions that impart desirable cosmetic properties to hair, in particular, curly hair. The hair cosmetic composition includes a non-ionic gum, a cationic guar gum, a cationic cellulosic quaternary ammonium compound, an acrylate-based polymer, a polyol and water. This reported composition is significantly different from the one described in the present invention by the way that, acrylate polymers and polyols are not part of the present invention.

CN108451778B discloses a transparent gel hair care composition. The transparent gel hair care composition comprising 0.1-50 % of cationic surfactant, 0.1-5 % of a humectant, 0.1-10 % of hair conditioning agent, 0.1-30 % of co-solvent, 0.05-1.0 % of preservative, 0.5-2.0 % of essence; the rest is deionized water. The composition of the invention has a transparent gel state, providing excellent moisturizing and conditioning effect for hair, effectively improving the wet impact of the hair, wet comb and dry comb performance, reducing the static effect of the hair, the hair is softer and smooth. This reported composition is significantly different from the one described in the present invention by the way that, humectants and co-solvents are not part of the present invention.

There is still an unmet need to provide a product that delivers hair conditioning while taking bath in water high in hardness. This is true for other applications as well including skin care such as face wash or body wash.

The present disclosure overcomes the aforesaid drawbacks of the prior art and other objects, features, and advantages of the present disclosure will now be described in greater detail.

OBJECTIVES:
An object of the present disclosure is to provide a cosmetic composition comprising an unsymmetrically substituted quaternary ammonium compound/s as cationic surfactant co-acting with one or more natural polymers and their derivatives, any one or more of non-ionic or cationic surfactants with emulsifying properties, and a fatty alcohol/s or combination thereof.

Another object of the present disclosure is to provide a synergistically co-acting composition which delivers superior conditioning benefit onto hair or skin irrespective of the hardness of water.

Yet another object of the present disclosure is to provide a synergistically co-acting composition, which synergistically and drastically improves conditioning of keratin fiber post shampoo wash using hard water.
Further object of the present disclosure is to provide a synergistically co-acting composition that is a safe, economically, and commercially viable proposition for domestic and industrial purposes.

Furthermore, an additional object of the present disclosure is to provide a synergistically co-acting composition in effective amounts together with cosmetically/ derma-pharmaceutically / acceptable vehicle/ carriers with or without other skin or hair care benefiting agents which is efficacious, safe and compatible to the skin and scalp with better consumer acceptance.

SUMMARY:
The present disclosure provides a synergistically co-acting composition which delivers superior conditioning benefit onto hair and skin, comparable or better than conditioner-only treatment to hair irrespective of the hardness of water being particularly advantageous in hard water conditions. The benefits of the co-acting composition were not disturbed by a subsequent surfactant application of a surfactant system such as shampoo.

An aspect of the present disclosure provides a conditioning composition for personal care comprising: a) an unsymmetrically substituted quaternary ammonium compound/s in a concentration ranging from 0.01 wt% to 20 wt%; and b) a non-ionic or cationic surfactant/s in a concentration ranging from 0 wt% to 20 wt%; and c) a natural polymer/s or derivatives of natural polymer/s in a concentration ranging from 0 wt% to 25 wt%; and d) a fatty alcohol/s having 8 to 18 carbons in a concentration ranging from 0 wt% to 5 wt%; and e) a cosmetically acceptable base, wherein the composition provides superior conditioning to hair and skin, even when used with hard water.

DETAILED DESCRIPTION
Specific details are given in the following description to provide a thorough understanding of the embodiments. However, it may be understood by any one of ordinary skill in the art that the embodiments may be practiced without being limited to these specific details.

All technical and scientific words used herein, unless otherwise defined, have the same meaning as commonly known by one having ordinary ability in the relevant field. The words "a" and "an" designate one or more (i.e., at least one) of the article's grammatical objects. Unless otherwise stated, weight is used to determine all percentages and ratios. All percentages and ratios are calculated based on the total final composition unless otherwise indicated.

As used herein, whether in a transitional phase or the body of a claim, the terms “comprise(s)” and “comprising” are to be interpreted as having an open-ended meaning. That is, the terms are to be interpreted synonymously with the phrases “having at least” or “including at least”. When used in the context of a process, the term “comprising” means that the process includes at least the recited steps but may include additional steps. When used in the context of a composition, the term “comprising” means that the composition includes at least the recited features or components but may also include additional features or components.

All composition described herein can be performed in suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the disclosure and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the disclosure.

The numerical values given for various physical parameters, dimensions and quantities are only approximate values and it is envisaged that the values higher than the numerical value assigned to the physical parameters, dimensions and quantities fall within the scope of the disclosure unless there is a statement in the specification to the contrary.

The following description includes various specific details and, is to be regarded as merely exemplary. Accordingly, those of ordinary skill in the art will recognize that without departing from the scope and spirit of the present disclosure and its various embodiments there may be any number of changes and modifications described herein.

Generally, hair conditioning was delivered using conditioning compositions (containing fatty alcohol/fatty alcohol mixture and a cationic surfactant as essential ingredients) either in combination with surfactant-based shampoo systems or as a standalone hair conditioning product. However, when hard water was used for hair rinse, compositions impart sticky feel onto hair fibre. Many conditioning compositions/formulations containing these two ingredients (fatty alcohol(s) and cationic surfactant) are available in the market. Apart from these, many hair conditioning compositions in the market with cationic surfactant, cationic polymers of natural or synthetic origin and chelating agents as actives are also known. Both these products (shampoo with conditioners or conditioners alone) are believed to deliver conditioning through coacervation mechanism as one of the ways to achieve the objective. Coacervate formation followed by its deposition onto hair can trigger deposition of other ingredients present in the medium.

However, it is a well-known fact that the quality of water strongly influences the final outcome. When water used during the usage of the products is hard the resulting hair looks dull (loss of lustre and shine), feels rough to touch, results in hair breakage, and predisposing hair to oxidation, causing heavy build-up of ingredients on the surface of hair. The hardness of water is most commonly expressed as milligrams of calcium carbonate equivalent per liter. Water containing calcium carbonate at concentrations below 60 mg/l is generally considered as soft; 60–120 mg/l, moderately hard; 120–180 mg/l, hard; and more than 180 mg/l, very hard. It is a challenge to have a conditioning product that is efficacious in all types of water. The present invention discloses a synergistically co-acting composition that effectively addresses this problem. The shampoo with conditioners or application of hair conditioners either as pre or post application of shampoo is to improve the clean feel along with improved conditioning of the hair. Hence one of the main objectives of the present invention is to achieve a synergistic composition that delivers significant improvement in conditioning delivery while using all types of water including hardwater.

Surprisingly, it was observed that, a composition comprising, CTAC, in combination with any one or more of ingredients selected from the group consisting of, any one or more of natural polymers, their derivatives, any one or more of surfactants with emulsifying properties, a fatty alcohol or their combination thereof, was found to deliver benefit of significant conditioning while using normal as well as hard water for washing keratin fibers.
An aspect of the present disclosure provides, a conditioning composition for personal care comprising: a) an unsymmetrically substituted quaternary ammonium compound/s in a concentration ranging from 0.01 wt% to 20 wt%; and b) a non-ionic or cationic surfactant/s in a concentration ranging from 0 wt% to 20 wt%; and c) a natural polymer/s or derivatives of natural polymer/s in a concentration ranging from 0 wt% to 25 wt%; and d) a fatty alcohol/s having 8 to 18 carbons in a concentration ranging from 0 wt% to 5 wt%; and e) a cosmetically acceptable base. Advantageously, the composition does not affect the performance of any hair care treatments such as shampoo wash, washing hair with two-in-one shampoo with inbuilt conditioner, conditioner application, hair straightening or strengthening or any hair care application involving a step of hair conditioning.

An embodiment of the present disclosure provides the unsymmetrically substituted quaternary ammonium compound/s are selected from the group consisting of Cetyltrimethylammonium chloride, Cetyltrimethylammonium bromide, Tetradecyltrimethylammonium chloride, Quaternium 82, Benzalkonium chloride, Soy ethyl morpholinium ethosulfate, N-Alkyl(C10-C18) dimethyl ethyl benzyl ammonium chloride, and N-Alkyl(C10-C18) dimethyl benzyl ammonium chloride or combination thereof.

In an embodiment of the present disclosure provides the non-ionic and cationic surfactant/s with emulsifying properties are selected from the group consisting of Stearamidopropyl Dimethylamine, PEG-120 Methyl Glucose Dioleate, Cocoamide monoethanolamine, Cocoamide DEA, Alkylglucosides, Alkylpolyglucosides, Laureth-23, Steareth-10, Steareth-20, Ceteaareth-20, Sorbitan esters, Polysorbates, Polyglyerol alkyl ether, Glucosyl dialkyl ethers, Crown ethers, Poloxamer, Hydrophobic non-ionic thickeners having low molecular weight or combination thereof.

Another embodiment of the present disclosure provides the natural polymer/s are selected from the group consisting of starches such as rice Starch, maize starch, potato starch, taro starch, corn starch; natural gums such as guar gum, xanthan gum, Acacia gum, tragacanth gum, karaya gum, ghatti gum, locust bean bum, fenugreek gum, tara gum, psyllium gum, cassia gum, konjac gum; and other polysaccharides such as carrageenan, alginates, alginic acid, glycogen, chitin, glucan, lentinan, agar, pectin, dextran, hyaluronic acid, heparin, lignin, fibroin, cutin, suberin, shellac, resilin, pectin, amylopectin and sporopollenin or combination thereof.

Yet another embodiment of the present disclosure provides the derivatives of natural polymers are selected from the group consisting of Hydroxyethyl cellulose, Cellulose ether, Guar and Hydroxy propyl trimonium chloride or combination thereof.
In yet another embodiment of the present disclosure provides the fatty alcohol/s having 8 to 18 carbons is selected from the group consisting of cetearyl alcohol, octanol, decyl alcohol, lauryl alcohol, myristyl alcohol, and stearyl alcohol or combination thereof.

Further embodiment of the present disclosure provides the cosmetically acceptable bases comprise among other benefit agents selected from the group consisting of moisturisers, damage repair agents, hair strengthening agents, anti-dandruff agents, nutrients, and other ingredients such as thickeners, opacifiers, pearlisers, antioxidants, water-hardness modifying agents, preservatives, fragrance, and colours / pigment or combination thereof.

Furthermore, embodiment of the present disclosure provides the composition having pH ranges from 4.0 to 8.5.

In an embodiment of the present disclosure provides the composition is in the form of a solution, emulsion, suspension, or gel used as a standalone product and an additional product in a personal care regimen.

In an embodiment of the present disclosure provides the synergistically co-acting composition utilizes commercially available chemicals which are safe to the skin and scalp at the suggested dosage levels. Thus, the composition and the technology of utilizing the same will be an economically and commercially viable proposition for multiple purposes such as personal care applications and for various industrial purposes.

In an embodiment of the present disclosure provides the composition and the technology of utilizing the same will be an economically and commercially viable proposition since the ingredients are commercially available and cheap.

In an embodiment of the present disclosure provides the cationic surfactant CTAC is known to deliver mild conditioning onto hair. Interestingly, formulations containing the individual ingredients II-V, by themselves or a combination of two of these at a time or more than two or all put together was not able to provide significant improvement in hair conditioning while using hard water for washing hair. Indeed, few of these formulations delivered conditioning to the hair fibres with stickiness to hair that could trigger series of hair related problems. However, these compositions when incorporated with required quantities of CTAC were able to reduce sticky feel on the hair and provide significant improvement in the conditioning of hair. Thus, the inventive composition works synergistically.

The inventive composition can be used along with any shampoo composition so as to deliver the significant improvement in hair conditioning while using hard water for hair rinsing.

The advantages and utility of the present invention are further illustrated by way of the following non-limiting examples. The stated amounts are based on weight.

EXAMPLES
The following are non - limiting examples of the composition described herein. The examples were prepared by the method described below. Other modifications of the present invention that are within the skill of those familiar in the techniques and art of formulation can be undertaken without departing from the spirit and scope of this invention.

All percentages mentioned are weight%. EXPT is an abbreviation of Experiment.

Preparation of Water of Known Hardness: Water of known hardness was prepared following CIPAC guidelines. The detailed description is given subsequently.
Estimation of Water Hardness: The hardness of water samples was estimated based on the method given by Indian Standards: IS 3025 (Part 21): 2009. (ICS 13.060.50). The method is based on the estimation of the hardness of water samples through titration with a standardized EDTA solution.

General Procedure 1: Preparation Of Synthetic Hard Water Of Known Hardness
I. Preparation Of Stock Solution: Water of known hardness was prepared following CIPAC guidelines.
Part A) 0.2M Ca2+ solution: Weigh accurately 20g of Calcium carbonate in a 1-liter conical flask and dissolve it in the minimum of distilled water. Place a small filter funnel in the mouth of the flask and add slowly 1 mol/lit hydrochloric acid (410 ml), while swirling the contents. When all the calcium carbonate is dissolved, dilute the solution to approximately 800 ml with distilled water, and boil gently to expel any carbon dioxide. Cool the solution, add methyl red indicator (2 drops) and neutralize to intermediate orange color with dropwise addition of 1 mol/lit ammonia solution. Transfer quantitatively to a 1000 ml volumetric flask and makeup to the volume mark with distilled water.
Part B) 0.2M Mg2+ solution: Weigh accurately 8.065g of Magnesium oxide in a 1liter conical flask and dissolve it in a minimum of distilled water. Place a small filter funnel in the mouth of the flask and add slowly 1 mol/lit hydrochloric acid (410 ml), while swirling the content. When all the magnesium oxide is dissolved, dilute the solution to approximately 800 ml. Add methyl red indicator (2 drops) and neutralize to intermediate orange color with 1 mol/lit ammonia solution, added dropwise. Transfer quantitatively to a 1000 ml volumetric flask and makeup to the volume with distilled water.
Preparation Of Lab Made Hard Water (LMHW): As prepared 65ml of 0.2M Ca2+ solution and 15ml of 0.2M Mg2+ solution was mixed in a 1 litre conical flask and volume was made up to 800ml, pH of the solution adjusted using dil. NaOH solution to 7.6-7.8 and made up to 1000ml. 600-650ml of this solution was withdrawn and made up to approx. 800ml, pH of the solution adjusted to 7.6-7.8 using dil. NaOH and made up to 1000mlin a volumetric flask. An aliquot of this sample is used as a control sample to estimate the hardness of the as “prepared synthetic water”. The water prepared is labeled as Lab Made Hard Water (LMHW), this abbreviation will be used herein after denoting the hard water sample prepared as described above. This water is used for rinsing hair swatches.
II. Estimation Of Titratable Water Hardness
For estimation of titratable water hardness, an aliquot of a water sample, maximum 50 ml, was pipette into a 250-ml conical flask. To this solution, 1 ml hydroxylamine hydrochloride (NH2OH.HCl) solution and 2 ml freshly prepared ammonia- ammonium chloride buffer solution were added. Few drops of Eriochrome black T indicator solution was added and titrated against 0.01M standard EDTA solution.

At the endpoint purple colour disappears with the appearance of clear sky blue colour. Blank titration, carried out similarly as that for the sample, is used for comparison.
Calculation:
Total hardness, ppm = (1000 x (V1-V2) x CF)/V3
wherein,
V1 = volume in ml of the EDTA standard solution used in the titration for the sample,
V2 = volume in ml of the EDTA solution used in the titration for blank,
V3 = volume in ml of the sample taken for the test.
CF = X1/X2 = correction factor for standardization of EDTA
X1 = volume in ml of standard calcium solution taken for standardization, and
X2 = volume of ml of EDTA solution used in the titration.
Each sample was repeated thrice to get the average value of the estimation of hardness.

General Procedure 2: Model Example of Preparing The Compositions Of The Present Invention for hair care
Two different types of general compositions were used for the different studies: (1) with preservative and fragrance and (2) without preservative and fragrance. These are listed in Table 1.
Table 1: Composition of Typical Formulation
S.No. Phase Ingredient General Formula #1 General Formula #2
%w/w %w/w
1 A DM Water Total Water – 10 As per relevant Table
Co-acting / Partner Compound As per relevant Table As per relevant Table
2 B CTAC (Cetrimonium Chloride) As per relevant Table As per relevant Table
3 C DM Water 10 -
Sodium benzoate As per relevant Table -
4 D Fragrance S2071 (Curly Hair Spa) As per relevant Table -
Total 100 100
Method of Preparation: This is illustrated using the composition General Formula #1 and General Formula #2 as given in Table 1.
Step-1: DM water was taken in a stainless vessel/beaker fitted with an overhead stirrer. The stirrer speed was set as to form a vortex. In the vortex, the co-acting/partner compound/s of Phase A were added slowly. Mixing was continued for 30 mins to ensure complete dispersion or dissolution.
Step-2: Phase-B material was added into the same stainless vessel and stirred for 3-5 minutes to obtain Phase AB.
Step-3(for General Formula #1): Separately, DM water (10ml) and sodium benzoate were taken in a beaker or in a stainless vessel. The contents were warmed to 50-60⁰C if required, and then cooled the contents to room temperature. The contents were added to Phase (AB) and were stirred for 3-5 minutes to obtain Phase ABC.
Step-4(for General Formula #1): Phase-D ingredients were added into Phase ABC and mixed for 10 minutes.

General Procedure 3: Evaluation Of Effectiveness of Tthe Present Invention in Hair care
The inventive compositions, prepared by following General Procedure 2, were assessed for their hair conditioning properties based on expert panel feedback.
Application of the Present Invention:
1. 4-6g of the product was applied to one-half of the head of a volunteer
2. 4-6g of DM water (as a placebo formulation) was applied to the other half of the head (reference side) of the volunteer.
3. Both the products were applied by gentle massage throughout the scalp and root to tip of the hair in their specified region
4. This was followed by shampooing on both sides using shampoo (without conditioning agent), followed by rinsing with hard water (either with lab made hard water or domestic hard water). The hair was dried using hair drier.
5. Hair on both sides of the head were evaluated by an expert panel who had no knowledge of what was applied on either side of the head, for hair conditioning effect and assigned a score of 1 to 10 to hair on each half of head.
Expert Panel: A panel of 10 individuals with an ability to differentiate different degrees of smoothness and tactile feels were identified from among the general public. They were further trained to evaluate and assign ratings to hair.
Rating Scale: A scale of 1-10 was used for rating with Rating-1being very poor conditioning and Rating-10 being excellent conditioning. The reference side (placebo formulation followed by shampooing side) was rated as giving a basal conditioning score of 1-2 in all the experiments.

Example #1: Studies On Conditioning of Hair (Lab studies) - Preparation And Evaluation Of Compositions Of Present Invention
Based on the general procedure described (General Procedure 2), different compositions were prepared and tested on hair swatches for their effect on conditioning (General Procedure 3).

Table 2A and Table 2Bshow different compositions having either a single component or the single component along with CTAC and their impact on conditioning of hair. The compositions are organized in sets for ease of comparison. The tables also show the conditioning effect of the compositions on hair.
Table 2A: Conditioning Effect of Combination of CTAC With Different Single Components
S.No. Ingredient Amount of ingredients used (wt%)
EXPT-3 Set #S1 Set #S2 Set #S3 Set #S4
EXPT-1 EXPT-2 EXPT-4 EXPT-5 EXPT-6 EXPT-7 EXPT-8 EXPT-9
1 CTAC 0.75 - 0.75 - 0.75 - 0.75 - 0.75
2 Incromine SD - 2 2 - - - - - -
3 Natrosol 250HHRPC - - - 1 1 - - - -
4 CSA - - - - - 0.5 0.5 - -
5 Cellulose ether - - - - - - - 1 1
6 Rice Starch - - - - - - - - -
7 Guar gum - - - - - - - - -
8 Jaguar C13 S - - - - - - - - -
9 PEG-120-MeGD - - - - - - - - -
10 Water 99.25 98 97.25 99 98.25 99.5 98.75 99 98.25
Total weight 100 100 100 100 100 100 100 100 100
Performance Measure of the Formulations
Water Hardness (ppm) 1000 1000 1000 1000 1000 1000 1000 1000 1000
Conditioning on Hair 3 4 8 4 8 4 8 4 8
Note #1: Conditioning was measured on a scale of 1 - 10, 10 being the best and 1 being the worst.
Note #2 (Abbreviations): CTAC = Cetrimonium Chloride; Incromine SD = Stearamidopropyl Dimethylamine; Natrosol 250 HHRPC= Hydroxyethyl cellulose; CSA = Cetearyl Alcohol; PPEG-120-MeGD = EG-120 Methyl Glucose Dioleate; Jaguar C13 S = Guar Hydroxypropyltrimonium Chloride

Table 2B: Conditioning Effect of Combination of CTAC With Different Single Components
S.No. Ingredient Amount of ingredients used (wt%)
Set #S5 Set #S6 Set #S7 Set #S8
EXPT-10 EXPT-11 EXPT-14 EXPT-15 EXPT-15-A EXPT-12 EXPT-13 EXPT-13-A EXPT-16 EXPT-17
1 CTAC - 0.75 - 0.75 0.75 - 0.75 0.75 - 0.75
2 Incromine SD - - - - - - - - - -
3 Natrosol 250 HHRPC - - - - - - - - - -
4 CSA - - - - - - - - - -
5 Cellulose ether - - - - - - - - - -
6 Rice Starch 10 10 - - - - - - - -
7 Guar gum - - 1 1 1 - - - - -
8 Jaguar C13 S - - - - - 1 1 1 - -
9 PEG-120-MeGD - - - - - - - - 4 4
10 Water 90 89.25 99 98.25 98.25 99 98.25 98.25 96 95.25
Total weight 100 100 100 100 100 100 100 100 100 100
Performance Measure of the Formulations
Water Hardness (ppm) 1000 1000 1000 1000 100 1000 1000 100 1000 1000
Conditioning on Hair 3 7 4 8 8 5 9 9 3 7
Note #1: Conditioning was measured on a scale of 1 - 10, 10 being the best and 1 being the worst.
Note #2 (Abbreviations): CTAC = Cetrimonium Chloride; Incromine SD = Stearamidopropyl Dimethylamine; Natrosol 250 HHRPC = Hydroxyethyl cellulose; CSA = Cetearyl Alcohol; PPEG-120-MeGD = EG-120 Methyl Glucose Dioleate; Jaguar C13 S = Guar Hydroxypropyltrimonium Chloride

Table 2C: Conditioning Effect of Combination of CTAC With Different Single Components
S.No. Ingredient Amount of ingredients used (wt%)
Set #S9 Set #S10 Set #S11 Set #S12
EXPT-18 EXPT-19 EXPT-20 EXPT-21 EXPT-22 EXPT-23 EXPT-24 EXPT-25
1 CTAC - 0.75 - 0.75 - 0.75 - 0.75
2 Octanol 0.5 0.5 - - - - - -
3 Lauryl alcohol - - 0.5 0.5 - - - -
4 Myristyl alcohol - - - - 0.5 0.5 - -
5 Stearyl alcohol - - - - - - 0.5 0.5
6 Water 99.5 98.75 99.5 98.75 99.5 98.75 99.5 98.75
Total weight 100 100 100 100 100 100 100 100
Performance Measure of the Formulations

Water Hardness (ppm) 1000 1000 1000 1000 1000 1000 1000 1000
Conditioning on Hair 3 7 3 7 3 7 3 7
Note #1: Conditioning was measured on a scale of 1 - 10, 10 being the best and 1 being the worst.
Note #2 (Abbreviations): CTAC = Cetrimonium Chloride;
An analysis of Set #S1 to Set #S12 clearly indicates that the combinations of CTAC, a quaternary ammonium cationic compound, with each of the
a) Non-ionic or cationic surfactants with emulsifying properties such as Incromine SD (Expt-2), PEG-120-MeGD (Expt-17),
b) natural polymers such as Rice Starch (Expt-11), Guar gum (Expt-15),
c) derivatives of natural polymers such as Natrosol 250 HHRPC (Expt-5), Cellulose ether (Expt-9), Jaguar C13 S (Expt-13), and
d) fatty alcohols such as Octanol, Lauryl alcohol, Myristyl alcohol, Stearyl alcohol, Cetearyl Alcohol with a carbon chain length of C-8 to C-18 carbons (Expt-7, Expt-19, Expt-21, Expt-23 and Expt-25) delivered far better conditioning compared to when these co-acting ingredients were present alone. This improvement is reflected in the assessment score given by the expert panel – the higher the score better is the conditioning. Thus, for example, while single ingredients CTAC (Expt-3) had a conditioning assessment score of 3 and the single component Incromine SD (Expt-1) had a conditioning assessment score of 4, a combination of these two ingredients as shown in Expt-2, had a conditioning assessment score of 8. This type of improvement is observed in all the sets #S1 to #S12.

Interestingly, Expt-13A & 15-A, show that, when soft water used for rinsing, the inventive compositions are also giving very good conditioning onto hair. Thus, the inventive compositions are equally effective in both soft and hard water. This is a unique technical advancement achieved by the present invention.

Additional compositions and experiments with different cationic compounds as shown in Tables 2D, 2E, 2F and 2G, demonstrate broader applicability of this serendipitous observation. It is shown that unsymmetrically substituted quaternary ammonium compounds.

such as Cetyltrimethylammonium bromide (CTAB), Tetradecyltrimethylammonium chloride, Benzalkonium chloride, Quaternium 82, Soyethyl morpholinium ethosulfate, N-Alkyl(C10-C18) dimethyl ethylbenzyl ammonium chloride, NAlkyl(C10-C18) dimethyl benzyl ammonium chloride were also shown synergistic coaction with
a) surfactants with emulsifying properties such as Incromine SD (Expt-33,41,49,57,65,73 and 81), PEG-120-MeGD (Expt-40, 48, 56, 64, 72, 80 and 88);
b) natural polymers such as Rice Starch (Expt-37, 45, 53, 61, 69, 77 and 85), Guar gum (Expt-38, 46, 54, 62, 70, 78 and 86);
c) derivative of natural polymer such as Natrosol 250 HHRPC (Expt-34, 42, 50, 58, 66, 74 and 82), Cellulose ether (Expt-36, 44, 52, 60, 68, 76 and 84), Jaguar C13 S (Expt-39, 47, 55, 63, 71, 79 and 87), and
d) fatty alcohol such as CSA (Expt-35, 43, 51, 59, 67, 75 and 83) as evidenced based on experimental data given in Table-2D to Table-2G (Experiments 26-88).
It is interesting to note that, the unsymmetrically substituted quaternary ammonium compounds were able to work synergistically with the select ingredients at a different ratio and produce good hair conditioning. As an example, the experimental results of CTAC at higher ratio is given below in Table-3A (Experiment Nos. 89-96).
Encouraged by the studies as mentioned above, additional experiments were conducted where 2 co-acting ingredients were used with CTAC. The compositions and the results are shown in Table 3A & 3B (Experiment Nos. 97-107).
Table 3A: Conditioning Effect of Combination of Varying Quantities of CTAC With Different Components
S. No. Ingredients Amount of ingredients used (wt%)
EXPT-89 EXPT-90 EXPT-91 EXPT-92 EXPT-93 EXPT-94 EXPT-95 EXPT-96 EXPT-97
1 CTAC 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5
2 Incromine SD 2 - - - - - - - 2
3 Natrosol 250 HHRPC - 1 - - - - - - 1
4 CSA - - 0.5 - - - - - 0.5
5 Cellulose ether - - - 1 - - - - -
6 Rice Starch - - - - 10 - - - -
7 Guar gum - - - - - 1 - - -
8 Jaguar C13 S - - - - - - 1 - -
9 PEG-120-MeGD - - - - - - - 4 -
10 Sodium Benzoate - - - - - - - - -
11 Fragrance H3474 - - - - - - - - -
12 Water 96.5 97.5 98 97.5 88.5 97.5 97.5 94.5 95
Total weight 100 100 100 100 100 100 100 100 100
Performance Measure of the Formulations
Water Hardness (ppm) 1000 1000 1000 1000 1000 1000 1000 1000 1000
Conditioning on Hair 8 8 8 7 7 8 9 7 6

Table 3B: Conditioning Effect of Combination of Varying Quantities of CTAC With Different Components
S. No. Ingredients Amount of ingredients used (wt%)
EXPT-98 EXPT-99 EXPT-100 EXPT-101 EXPT-102 EXPT-103 EXPT-104 EXPT-105 EXPT-106 EXPT-107
1 CTAC 1.5 5 5 10 10 16.6 10 16.6 10 16.6
2 Incromine SD - - - - - - - - - -
3 Natrosol 250 HHRPC 0.5 - 1 5 - - - - - -
4 CSA 0.5 - - - - - - - - -
5 Cellulose ether - 1 - - 5 5 - - - -
6 Rice Starch - - - - - - 20 20 - -
7 Guar gum - 1 - - - - - - 5 5
8 Jaguar C13 S - - - - - - - - - -
9 PEG-120-MeGD - - - - - - - - - -
10 Sodium Benzoate - 0.15 0.15 - - - - - - -
11 Fragrance H3474 - 0.6 0.6 - - - - - - -
12 Water 97.5 92.25 93.25 85 85 78.4 70 63.4 85 78.4
Total weight 100 100 100 100 100 100 100 100 100 100
Performance Measure of the Formulations
Water Hardness (ppm) 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000
Conditioning on Hair 6 7 7 7 7 7 7 7 8 8
Results from Expt-97& 98 (Table 3A & 3B) demonstrate that even a combination of two or three such ingredients co-acting with CTAC gives a synergistic boost to the composition resulting in superior conditioning of hair, as can be seen by the enhanced score given by the expert panel to the combined composition.

Expt-99 & 100 (Table 3B), albeit with different compositions, are also relevant in demonstrating that inclusion of additional ingredients, in these cases a preservative and a fragrance, does not interfere with the synergy of the combination and still delivers superior conditioning to hair. Thus, one can conclude that the inventive compositions are synergistic with no interference from the other formulation ingredients.

To understand the impact of higher levels of the synergistic components, additional experiments were performed that are captured in Table 3B.

The results of Expt-100, Expt-101 and Expt-102 to Expt-107 show that increased quantities of CTAC as well as that of the co-acting synergistic ingredients do not decrease the superior conditioning delivery and such combinations continue to deliver good conditioning to hair. This is demonstrated by the high rating of 7 – 8 given by the expert panel during assessment.

It may be noted that the results shared here show that the effects are observed over a wide range of concentrations of different ingredients. The concentrations used in these experiments are only illustrative and are not limiting.

The present disclosure provides for significant improvement in conditioning delivery while using hard water for hair rinse. The components of the composition co-act synergistically to deliver the benefits thereby enabling the consumer to overcome certain shortcomings of using hard water on which they have little control.

It can be understood based on the above given data that all the select combinations of ingredients possess a certain degree of enhanced hair conditioning behavior in hard water. However, one cannot conclude that all the cosmetic ingredients classified under these categories can deliver synergistic activity. This fact can be understood based on Expt-108 to Expt-117 data given below in Table 4. This substantiates the selective nature of the claimed composition.
Table 4: Conditioning Effect of Combination of CTAC With Different Cosmetic Ingredients
S.No. Ingredient Amount of ingredients used (wt%)
EXPT-108 EXPT-109 EXPT-110 EXPT-111 EXPT-112 EXPT-113 EXPT-114 EXPT-115 EXPT-116 EXPT-117
1 CTAC 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5
2 Carbopol 0.5 - - - - - - - - -
3 Aqua SF 1 0 1 - - - - - - - -
4 Sodium Alginate - - 2 - - - - - - -
5 Sodium CMC - - - 2 - - - - - -
6 Pectin - - - - 0.5 - - - - -
7 PQ-37 - - - - - 1 - - - -
8 Quaternium-22 - - - - - - 0.5 - - -
9 PQ-10 - - - - - - - 1 - -
10 Aculyn 60 PEG 150 Distearate - - - - - - - - 2 -
11 Maize Starch - - - - - - - - - 10
12 Sodium Benzoate 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15
13 Fragrance H3474 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6
14 Water 97.25 96.75 95.75 95.75 97.25 96.75 97.25 96.75 95.75 87.75
Total weight 100 100 100 100 100 100 100 100 100 100
Performance Measure of the formulations
Water Hardness (ppm) 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000
Conditioning on Hair 4 5 5 4 5 4 5 4 4 5
Note #1: Conditioning was measured on a scale of 1 - 10, 10 being the best and 1 being the worst.
Note #2 (Abbreviations): CTAC = Cetrimonium Chloride; Carbopol = ??; Aqua SF1 = Acrylates Copolymer; Sodium CMC = Sodium carboxymethyl cellulose; PQ 37 = Structure PQ37P?????; PQ10 = Celquat SC 230 M
Thickening agents such as Carbopol 990 (EXPT-108), Aqua SF 1 (Acrylates Copolymer) (EXPT-109), Cationic polymers cum thickening agents such as Structure PQ37P (PQ-113) (EXPT-36), Celquat SC 230 M(PQ-10) (EXPT-115), Natural polymers and their derivatives such as Sodium Alginate(EXPT-110), Sodium CMC(EXPT-111), Pectin(EXPT-112), and Maize Starch (INCI Name: Zea mays (Corn starch)) (EXPT-117), cationic surfactants such as Ceraphyl 60 (Quaternium-22)(EXPT-114), Surfactants with emulsifying properties such as Aculyn 60( PEG-150 Distearate) (EXPT-116) were not able to co-act synergistically with the cationic surfactant (CTAC) to give the desired viscosity, improvement in hair conditioning delivery. Thus, this inventive composition of the present application is selective.

Example #2: Instrumental Evaluation of Hair Conditioning by the Inventive Formulation
Delivery of conditioning of inventive formulations was evaluated against standard commercially available hair conditioner using Miniature Tensile Tester (MTT) [Make: Diastron].

For the study, along with the formulation of the present invention, a shampoo and a conditioner were also prepared. The details are given hereafter (Table 5, Table 6 and Table 7).

Example #2A: Preparation of The Test Sample (T1) of the Present Invention: The composition (T1) is described in Table 5.
Table 5: Composition Of Inventive Formulation for Testing Using Instruments
S.No. Phase Ingredient EXPT-118 (T-1) EXPT-119 (T-4) EXPT-120 (T-5)
%w/w %w/w %w/w
1 A Water 28 91.95 28
Jaguar C13 S (Guar Hydroxypropyltrimonium Chloride) 1.25 - 1.25
Water 62.7 - 67.7
2 B CTAC (Cetrimonium Chloride) 5 5 -
3 C DM Water 2 2 2
Sodium benzoate 0.2 0.2 0.2
4 D Panthenol 0.25 0.25 0.25
Fragrance 0.6 0.6 0.6
Total 100 100 100
Method of Preparation of Test Sample (T1):
Step-1: DM water (20ml) was taken in a stainless vessel/beaker fitted with an overhead stirrer. The stirrer speed was set as to form a vortex. In the vortex, Jaguar C13 S was added slowly and stirring was continued till a uniform gel was formed. To this gel, additional DM water (62.7 ml) was added and stirring continued for 30 min.
Step-2: Add CTAC into the stainless vessel and stir for 3-5 minutes.
Step-3: Separately, DM water (2ml) and sodium benzoate were taken in a beaker or in a stainless vessel. The contents were dissolved well without any particles and were added to Phase (AB) and were stirred for 3-5 minutes.
Step-4: Panthenol and Fragrance were added into Phase ABC and mixed for 10 minutes to give the Test Sample (T1).

Samples T-4 and T-5 were prepared by following a similar protocol except that the indicated missing ingredients were not added; the corresponding steps would get modified accordingly.

Example #2B: Preparation of Conditioner Sample (T2): The composition T2 is described in Table 6.
Table 6: Composition of Conditioner Formulation (T2) For Testing Using Instruments
S.No. Phase Ingredient EXPT-121
%w/w
1 Water Phase Water 82.7
Disodium EDTA 1
Glycerin 2.5
Behentrimonium chloride 1
2 Oil Phase Cetearyl alcohol 5
Quaternium-91 (and) Cetrimonium Methosulfate (and) Cetearyl Alcohol 1
Paraffin 1
Ethylhexyl Methoxycinnamate 0.5
ButyrospermumParkii (Shea) Butter 0.25
Cetrimonium Chloride 0.5
3 Other Ingredients Phenoxyethanol (and) Ethylhexylglycerin 0.5
Polydimethylsiloxanes 3
Panthenol 0.25
Fragrance 0.8
Total 100
Method of Preparation of Conditioner Sample (T2): The procedure for preparing the conditioner composition T2 is given below.
1. Preparing the Water Phase: In a stainless-steel container, DM water was taken and Disodium EDTA, Glycerin, and Behentrimonium Chloride were added with gentle stirring. Heat the mixture to 80-85°C until all ingredients dissolved completely.
2. Preparing the Oil Phase: In a separate stainless steelcontainer,Cetearyl Alcohol, Quaternium-91, Cetrimonium Methosulfate, Cetearyl Alcohol, Paraffin, Ethylhexyl Methoxycinnamate,andButyrospermumParkii (Shea) Butterwere taken. This was heated to 70-75°C until it was completely melted.
3. Combining Phases: Gradually add the oil phase to the water phase while mixing with a homogenizer to ensure proper blending. Then add Cetrimonium chloride to oil phase and transfer to the water phase while mixing with a homogenizer for 20 minutes.
4. Cooling Down: Allow the combined mixture to cool while stirring continuously until it reaches 40°C.
5. Adding Remaining Ingredients: Finally, the remaining ingredients, including, Phenoxyethanol (and) Ethylhexylglycerin,Polydimethylsiloxanes and other hair-benefiting ingredients panthenol, were added into the cooled mixture. Then fragrance was addedand mixed for 15 minutes to ensure even mixing of all components, the composition was mixed thoroughly to obtain T2.
Example #2C: Preparation of Shampoo Sample (T3): The composition of T3 is described in Table 7.
Table 7: Composition Of Shampoo Formulation (T3) For Testing Using Instruments
S.No. Phase Ingredient EXPT-122
%w/w
1 Base Ingredients Phase Sodium LaurethSulfate26% 50
Cocamide MEA 1
Glycol Distearate 1.25
Ethylhexyl Methoxycinnamate 0.5
Glycerine 0.5
Disodium EDTA 0.1
Water 4.875
2 Gel phase 1 Carbomer 0.3
Citric Acid 0.075
Water 15
3 pH adjuster & Base ingredients phase Sodium Hydroxide 0.15
Water 0.45
Cocamidopropyl Betaine 2
4 Gel phase 2 Guar Hydroxypropyltrimonium Chloride 0.2
Water 10
5 Other Ingredients & Viscosity adjuster Phase DMDM Hydantoin 0.25
Polydimethylsiloxanes 6.5
Fragrance 0.8
Mica and Titanium Dioxide 0.05
Water 1
Sodium Chloride 1
Water 4
Total 100
Procedure of Preparation of The Shampoo Sample (T3): The procedure for preparing the shampoo composition T3 is given below
1. Combining Base Ingredients: In a Stainless steel beaker, combine ingredients of Sodium Laureth Sulfate, Cocamide MEA, Glycol Distearate, Ethylhexyl Methoxycinnamate, Glycerin, Disodium EDTA with 5 ml of Deionized water. The resultant mixture was heated to 70-75°C until fully melted, then cooled it to 50°C.
2. Adding Carbomer: Carbomer was pre-dispersed in water with citric acid, and then it was added to the stainless-steel beaker and mixedthoroughly for 10 minutes.Adjust the pH sodium hydroxide with water between 5 and 6. Add cocamidopropyl betaine in a stainless-steel beaker and mix well for 2 minutes.
3. Incorporating Cationic Polymers:Guarhydroxypropyltrimonium chloridewas pre-dispersed in water and then was added to the stainless steel beaker , while mixing well for 10 minutes.
4. Adding Remaining Ingredients: Gradually other ingredients were added one by one at 40°C, DMDM Hydantoin, Polydimethylsiloxanes & Fragrance. Mi mixing thoroughly after each addition for 5 minutes.
5. Adding Aesthetic ingredients: Mica and titaniumdioxidewas premixed in water and then was added tostainless steel beaker and mixedthoroughly for 5 minutes.
6. Building Viscosity: Finally, Sodium Chloride was added to achieve the desired viscosity and obtain the shampoo sample T3.
Example #2D: The efficacy of the inventive formulation was evaluated by an instrumental method in hair swatches using Miniature Tensile Tester (MTT). The effectiveness was evaluated based on co-efficient of friction (COF) root to tip reflecting the requirement of the hair friction (smoothness) Ref: WO2016189276A1 - Hair Care Formulation (Combing and Friction Accessories, Croda International), December 2016."
The inventive composition was tested against 2 different protocols: (a) Pre-wash Treatment and (b) Post-wash Treatment.
Pre-wash Treatment:
(1) Treatment of hair swatch with test sample T1 followed by shampoo wash with T3;
(2) Treatment of hair swatch first with conditioner sample T2 followed by wash with the shampoo sample T3;
(3) Treatment of hair swatch first with test sample T4 followed by wash with the shampoo sample T3; and
(4) Treatment of hair swatch first with test sample T5 followed by wash with the shampoo sample T3.
Post-wash Treatment:
(1) Treatment of hair swatch with shampoo wash T3 followed by test sample T1; and
(2) Treatment of hair swatch first with the shampoo sample T3 followed by the conditioner sample T2.
In both cases, the conditioner was used as an alternative to the test sample to study any advantage of the inventive composition (test sample) over the conditioners.
The results of the study - in both the pre and post wash cases are given in Table 8.
Table 8: Treatment Sequence and Effectiveness of Inventive Composition by Measuring Hair friction (smoothness)
Treatment Sequence Treatments Given to The Hair Swatches CODE Percentage change (%) in Smoothness Statistical significance
Root to tip
1st Treatment 2nd Treatment Value STD.DEVIATION
Pre-wash Treatment Test Sample Shampoo T1-T3 -32.59 21.32 Significant
Conditioner Shampoo T2-T3 -25.74 15.5 Significant
Test Sample Shampoo T4-T3 -15.77 13.68 Not Significant
Test Sample Shampoo T5-T3 -10.64 5.87 Not Significant
Post-wash Treatment Shampoo Test Sample T3-T1 -39.66 5.22 Significant
Shampoo Conditioner T3-T2 -2.25 6.84 Not Significant
Note: The % change represents change in the hair friction (smoothness) between untreated hair swatch and after treatment of hair swatch.
It may be noted from the results, that whether pre-wash treatment or post-wash treatment, the inventive composition reduced the combing force i.e. co-efficient of friction more significantly than when conditioner was used instead (-32.59 vs -25.74 and -39.66 vs -2.25). Further, it was noted that the post-wash treatment performed better than the pre-wash treatment (-39.66 vs -32.59). All the critical values were statistically significant. The components of the inventive compositions viz. CTAC (Test sample:T-4)(Expt-119) andGuar Hydroxypropyltrimonium Chloride(Test sample:T-5) (Expt-120) were tested similarly using MTT. The pre-wash treatment results proved the synergistic coaction of the ingredients. The combination of these two ingredients proven to produce less friction of-32.59% Vs -15.77% and -10.64% (for CTAC and Guar Hydroxypropyltrimonium Chloride respectively).

Thus, one can conclude that the inventive formulation delivers statistically significant conditioning delivery compared to standard commercially available conditioner applied to human hair swatches.

Based on the above experimental data, one can clearly establish the merit of the present disclosure that it provides for significant improvement in conditioning delivery while using hard water for hair rinse. It is further established that the components of the composition co-act synergistically to deliver the benefits even while using hard water for hair treatment.

General Procedure 4: Model Example of Preparing the Compositions of The Present Invention for Personal Care
Two different types of general compositions for personal care purpose were used for the different studies: (1) with the actives of the present invention, preservative and fragrance and (2) without the actives of the present invention, with preservative and fragrance. The compositions of these are listed in Table 9.
Table 9: Personal care Composition
S.No. Phase Ingredient EXPT-123 EXPT-124 EXPT-125
%w/w %w/w %w/w
1 Base Ingredients Phase Sodium LaurethSulfate26% 23.07 23.07 23.07
Glycerin 5.00 5.00 5.00
Disodium EDTA 0.10 0.10 0.10
Water 25.00 25.00 4.88
2 Gel phase 1 Jaguar C13S 0.50 - -
Natrosol 250 HHR PC - 0.50 -
Water 15.00 15.00 15.00
3 pH adjuster & Base ingredients phase Citric acid 0.05 0.05 0.05
Water 0.45 0.45 0.45
CTAC 1.00 1.00 -
Cocamidopropyl Betaine 15.00 15.00 15.00
4 Other Ingredients Phase Euxyl PE 9010 0.50 0.50 0.50
Fragrance 0.50 0.50 0.50
Panthenol 0.25 0.25 0.25
Water 13.58 13.58 35.20
Total 100.00 100.00 100.00
Procedure of Preparation of The Personal Care composition:
1. Combining Base Ingredients: In a Stainless steel beaker-1, the ingredients Sodium Laureth Sulfate, Glycerin, Disodium EDTA were combined with 25 ml of Deionized water. The resultant mixture was mixed well for 10 minutes
2. Gel Phase 1: In a separate stainless beaker-2, pre-dispersed Jaguar C13S in water (Expt-123) or pre-dispersed Natrosol 250 HHRPC in water (Expt-124) or only water (Expt-125) was prepared and then it was added to the stainless steel beaker #1 containing base ingredients and mixed thoroughly for 10 minutes.
3. pH adjustment: Adjust the pH of the contents in stainless beaker-1 between 5 and 6 using Citric acid in water. Add CTAC and cocamidopropyl betaine into the stainless beaker-1 and mix well for 5 minutes
4. Adding Remaining Ingredients: Gradually other ingredients were added one by one Euxyl PE 9010, Fragrance, Panthenol and water into the stainless steel beaker, the contents were mixed thoroughly after each ingredient addition for 5 minutes to obtain a personal care composition.
General Procedure 5: Evaluation Of Effectiveness of the Present Invention in Skin care
The inventive compositions, prepared by following General procedure #4, as given in Expt-123, 124 and 125, were assessed for their skin conditioning properties based on expert panel feedback.
Application of the present Invention:
1. 4-5g of a product (say test sample) was applied to one of the fore arm of a volunteer, other forearm another product (say formulation without actives) was applied
2. Both the products were applied by gentle massage throughout the forearm and gently lathered
3. This was followed by washing of both the forearms using about 400ml of water with hardness of 1000 ppm, by using about 75-100ml portions each time, rinsing repeated four times. Followed by rinsing with hard water (either with lab made hard water or domestic hard water) 100ml. The excess water was allowed to drip, thus forearm was air dried naturally to dryness.
4. Expert panel observed the lathering and feel on both forearms were evaluated by an expert panel by touching the skin of the volunteers with right hand evaluating right hand and left hand evaluating left forearm of the volunteer to avoid cross contamination. The expert panelists had no knowledge of what was applied on either side of the forearm, for skin conditioning effect and assigned a score of 1 to 10. Further the expert panel was asked to give their overall feel on the skin treated with the product in terms of shine/clean feel.

Expert Panel: A panel of 10 individuals with an ability to differentiate different degrees of smoothness, stickiness and tactile feels were identified from among the public. They were further trained to evaluate and assign ratings to skin.
Rating Scale: A scale of 1-10 was used for rating with Rating-1 being No stickiness and Rating-10 being too much stickiness on skin. A scale of 1-10 was used for rating with Rating-1 being No shine, soiled, uncleaned looking skin and Rating-10 being well cleaned and conditioned looking skin.
Table10: Results Of Skin Conditioning Study
Performance measure of formulations
Parameters Expt-123 Expt-124 Expt-125
Lathering on skin while applying the product 7 7 6
Stickiness feels on skin after drying 3 3 6
Shine/clean feel score 7 8 5
Thus, one can understand that the compositions incorporated with synergistically co-acting ingredients (Expt-123 & EXPT-124) able to render skin looking well cleaned and devoid of stickiness. Thus, the personal care application of the inventive composition demonstrated its efficacy even in hard water.

Thus, one can conclude that the inventive formulation delivers significant conditioning delivery compared to standard commercially available personal care products applied to human hair/skin.

Based on the above experimental data, one can clearly establish the merit of the present disclosure that it provides for significant improvement in conditioning delivery onto hair and skin while using hard water for rinse. It is further established that, the components of the composition co-act synergistically to deliver the benefits even while using hard water for hair or treatment.

ADVANTAGES:
1. The present disclosure is to provide a synergistically co-acting composition that is a safe, economically, and commercially viable proposition for domestic and industrial purposes.
2. The present disclosure is to provide a synergistically co-acting composition, which synergistically and drastically improves conditioning of keratin fiber post shampoo wash using hard water.
3.The present disclosure is to provide a synergistically co-acting composition which delivers superior conditioning benefit onto hair or skin irrespective of the hardness of water.
4. The present disclosure does not affect the performance of any hair care treatments such as shampoo wash, washing hair with a two-in-one shampoo with an inbuilt conditioner, conditioner application, or hair straightening or strengthening or any hair care application involving a step of hair conditioning.

, Claims:We Claim,

1. A conditioning composition for personal care comprising:
a) an unsymmetrically substituted quaternary ammonium compound/s in a concentration ranging from 0.01 wt% to 20 wt%; and
b) a non-ionic or cationic surfactant/s in a concentration ranging from 0 wt% to 20 wt%; and
c) a natural polymer/s or derivatives of natural polymer/s in a concentration ranging from 0 wt% to 25 wt%; and
d) a fatty alcohol/s having 8 to 18 carbons in a concentration ranging from 0 wt% to 5 wt%; and
e) a cosmetically acceptable base,

wherein the composition provides superior conditioning to hair and skin, even when used with hard water.

2. The composition as claimed in claim 1, wherein the unsymmetrically substituted quaternary ammonium compound/s are selected from the group consisting of Cetyltrimethylammonium chloride, Cetyltrimethylammonium bromide, Tetradecyltrimethylammonium chloride, Quaternium 82, Benzalkonium chloride, Soy ethyl morpholinium ethosulfate, N-Alkyl(C10-C18) dimethyl ethyl benzyl ammonium chloride, and N-Alkyl(C10-C18) dimethyl benzyl ammonium chloride or combination thereof.

3. The composition as claimed in claim 1, wherein the non-ionic and cationic surfactant/s with emulsifying properties are selected from the group consisting of Stearamidopropyl Dimethylamine, PEG-120 Methyl Glucose Dioleate, Cocoamide monoethanolamine, Cocoamide DEA, Alkylglucosides, Alkylpolyglucosides, Laureth-23, Steareth-10, Steareth-20, Ceteaareth-20, Sorbitan esters, Polysorbates, Polyglyerol alkyl ether, Glucosyl dialkyl ethers, Crown ethers, Poloxamer, Hydrophobic non-ionic thickeners having low molecular weight or combination thereof.

4. The composition as claimed in claim 1, wherein the natural polymer/s are selected from the group consisting of starches such as rice Starch, maize starch, potato starch, taro starch, corn starch; natural gums such as guar gum, xanthan gum, Acacia gum, tragacanth gum, karaya gum, ghatti gum, locust bean bum, fenugreek gum, tara gum, psyllium gum, cassia gum, konjac gum; and other polysaccharides such as carrageenan, alginates, alginic acid, glycogen, chitin, glucan, lentinan, agar, pectin, dextran, hyaluronic acid, heparin, lignin, fibroin, cutin, suberin, shellac, resilin, pectin, amylopectin and sporopollenin or combination thereof.

5. The composition as claimed in claim 1, wherein the derivatives of natural polymers are selected from the group consisting of Hydroxyethyl cellulose, Cellulose ether, Guar and Hydroxy propyl trimonium chloride or combination thereof.

6. The composition as claimed in claim 1, wherein the fatty alcohol/s having 8 to 18 carbons is selected from the group consisting of cetearyl alcohol, octanol, decyl alcohol, lauryl alcohol, myristyl alcohol, and stearyl alcohol or combination thereof.

7. The composition as claimed in claim 1, wherein the cosmetically acceptable bases comprise among other benefit agents selected from the group consisting of moisturisers, damage repair agents, hair strengthening agents, anti-dandruff agents, nutrients, and other ingredients such as thickeners, opacifiers, pearlisers, antioxidants, water-hardness modifying agents, preservatives, fragrance, and colours / pigment or combination thereof.

8. The composition as claimed in claim 1, wherein the composition having pH ranges from 4.0 to 8.5.

9. The composition as claimed in claim 1, wherein the composition is in the form of a solution, emulsion, suspension, or gel used as a standalone product and an additional product in a personal care regimen.