Description:SYNERGISTIC MITICIDAL COMPOSITION

FIELD OF INVENTION

The present invention relates to a novel, synergistic, stable miticidal composition for controlling mites in agricultural crops. The present invention further relates to a process for preparing the miticidal composition as a liquid formulation.

BACKGROUND OF THE INVENTION
Mites are minuscule members of the arachnid family, although they often get labeled as insects. Not only do they feed on foliage and suck out necessary nutrients, but they are also capable of carrying dangerous diseases. Some types of mites include spider, spruce, two-spotted, and European red mites. The visible signs of mite damage to the foliage include brown, wilted leaves; curled, yellow leaves; speckled foliage; leaf dropping; tiny, web-like structures; and mottled or dis-coloured needles on conifers, such as pine.

Miticides, also known as acaricides, are a type of pesticide specifically designed to kill mites. They are used in agriculture, horticulture, and other settings to protect crops and livestock from mite infestations. Miticides can act through different mechanisms, such as contact action, i.e., kill mites on contact with the plant or animal; fumigant action, i.e., some miticides can vaporise and be inhaled by mites, affecting their respiratory system; and systemic action, the plant absorbs some miticides and can be ingested by mites when they feed, providing longer-lasting protection.

Some miticides are selective, targeting specific mite species, while others are broader spectrum and can control various pests. Miticides are essential in agricultural settings to protect crops from significant damage caused by mites, which can result in substantial yield losses. They help manage and reduce mite populations, preventing outbreaks and infestations. They improve crop quality and enhance marketability by enhancing the overall health and appearance of the crops. To address this problem, the inventors have developed a novel, synergistic miticidal composition comprising the active ingredient combination of Hexythiazox and Etoxazole, which has a synergistic effect and provides excellent control of mites in crops, particularly in Chilli and Tea. The present invention composition provides a miticidal composition that delivers an enhanced miticidal action as compared to that obtained through the application of the solo active formulations.
OBJECT OF THE INVENTION
It is an object of the present invention to provide a novel, stable, synergistic, miticidal composition comprising a) Hexythiazox as the first active ingredient b) Etoxazole as the second active ingredient and at least one agrochemically acceptable excipient.

It is yet another object of the present invention to provide a ready-to-use miticidal composition that delivers an enhanced miticidal action at a reduced active dosage as compared to the combined recommended dosage of existing solo active ingredient formulations.

It is yet another object of the present invention is to provide a stable co-formulation of Hexythiazox and Etoxazole.

It is yet another object of the present invention to provide a liquid formulation comprising the active combination of Hexythiazox and Etoxazole.

It is yet another object of the present invention to provide a novel, stable, synergistic miticidal composition that is non-phytotoxic and environmentally safe.

It is yet another object of the present invention to provide a process of preparing the present miticidal composition in the form of a stable liquid formulation including an Oil Dispersion (OD) and Microemulsion Concentrate (MEC).

SUMMARY OF THE INVENTION
Accordingly, in one aspect, the present invention provides a novel, stable, synergistic miticidal composition comprising a) Hexythiazox as the first active ingredient b) Etoxazole as the second active ingredient and at least one agrochemically acceptable excipient.

In another aspect, the miticidal composition of the present invention comprises a) Hexythiazox, the first active ingredient in an amount ranging from 1-25% w/w b) Etoxazole, the second active ingredient in an amount ranging from 5-30% w/w and c) at least one agrochemically acceptable excipient.
In another aspect, at least one agrochemically acceptable excipient is selected from the group comprising an emulsifier, a surfactant, a co-surfactant, a stabilizer, a rheology modifier, a solvent, a co-solvent, anticaking agent, and demineralised water.

In another aspect of the present invention, the solvent may be selected from comprising of normal butanol (NBA), Dimethyl sulfoxide (DMSO), N- methyl pyrrolidone (NMP), aromatic hydrocarbon solvents, and cyclohexanone.

In another aspect of the present invention, the co -solvent is selected from the group comprising of Dimethyl sulfoxide (DMSO), soybean or palm oil methyl ester, benzyl alcohol, N-Methylpyrrolidone (NMP), xylene, methanol, ethanol, acetophenone, 2-ethylhexyl acetate, cyclohexyl acetate, cyclohexanone, isopropanol, mineral oil, ester derived from lactic acid.

In yet another aspect of the present invention, the emulsifier is selected from the group comprising of polyol esters, glycerol ethers, oxyethylenated and/or oxypropylenated ethers, and ethylene glycol polymers.

In another aspect of the present invention, the stabilizer is selected from the group comprising of vegetable oils, natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated shortchain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6-C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters.

In another aspect the miticidal composition comprises Hexythiazox in an amount ranging from 1-25% w/w, Etoxazole in an amount ranging from 5-30% w/w, stabilizer in an amount ranging from 1-8% w/w, solvent in an amount ranging from 10-40%w/w, co-solvent in an amount ranging from 1-10% w/w, emulsifier in an amount ranging from 15-40% w/w, and demineralized water in an amount ranging from 0-20% w/w.
In another aspect of the present invention, the miticidal composition comprises Hexythiazox in an amount ranging from 1-25% w/w; Etoxazole in an amount ranging from 5-30% w/w; rheology modifier in an amount ranging from 0.5-5% w/w, surfactant in an amount ranging from 5-20% w/w; stabilizer in an amount ranging from 10 -30% w/w, emulsifier in an amount ranging from 2-15% w/w, and solvent in an amount ranging from 25-60% w/w.

In another aspect of the present invention, the rheology modifier is selected from the group comprising hydrophilic fumed silica), silicon dioxide, magnesium alumina meta silicate, hydrophobic silica, colloidal silica, precipitated silica, hydrophobic silica powder and mixtures thereof.

In another aspect of the present invention, the surfactant is selected from the group comprising mono unsaturated fatty acids (cis-9-octadecanoic acid), polyoxyethylene alkyl ether, trisiloxane ethoxylate, polysorbates, ethoxylated tristyrylphenol phosphate, sodium lauryl sulphate and sodium methyl oleoyl taurate.

In another aspect of the present invention, the stabilizer is selected from the group comprising vegetable oil ethoxylate; natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated short chain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6- C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester.

In another aspect of the present invention, the emulsifier is selected from the group comprising vegetable oil ester alkoxylates, ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester.
In another aspect, the present invention provides a method of preparing the miticidal micro emulsion concentrate, the method comprising: dissolving Etoxazole technical in an amount ranging from 5-30% w/w in a solvent in an amount of 10 to 40% w/w to obtain a first solution;
adding Hexythiazox technical in an amount ranging from 1-25% w/w into the first solution to obtain a second solution; adding and mixing a co-solvent in an amount ranging from 1-10% w/w to the second solution; subsequently adding stabiliser in an amount ranging from 1-8% w/w and an emulsifier in an amount ranging from 15-40% w/w, and thereafter adding demineralized water to obtain a microemulsion concentrate.

In another aspect, the present invention provides a method of preparing the miticidal oil dispersion the method comprising: adding and mixing stabilizer in an amount ranging from 10-30% w/w, surfactant in an amount ranging from 5-20% w/w, and an emulsifier in an amount ranging from 2-15% w/w, in a solvent in an amount of 25-60% w/w to obtain a mixture; adding Etoxazole technical in an amount ranging from 5-30% w/w and Hexythiazox technical in an amount ranging from 1-25% w/w, to the mixture under continuous stirring to obtain a homogeneous mixture; grinding the mixture to obtain a mean particle size of less than 12 microns; and mixing and adding a rheology modifier in an amount ranging from 0.5-5% w/w to obtain an oil dispersion.

In another aspect, the present invention provides a novel, stable, synergistic miticidal composition that is highly effective in controlling mites in variety of agricultural crops, particularly in tea, coffee, and chilli.

In another aspect, the present invention provides a method if improving plant growth by applying a miticidally effective amount of the miticidal composition on a plant or a part thereof or at the locus or on the surface of plant propagation material.

In yet another aspect, the present invention provides a process for preparing said miticidal composition in the form of a stable liquid formulation including an Oil Dispersion (OD) and Micro emulsion Concentrate (MEC).

DETAILED DESCRIPTION OF THE INVENTION:
Those skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, compositions, and compounds referred to or indicated in this specification, individually or collectively, and all combinations of any two or more of said steps or features.

Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning that is commonly understood by one of ordinary skill in the art to which the invention belongs. For further guidance, term definitions may be included to better appreciate the teaching of the present invention.

As used herein, the term “plant” refers to any plant or part thereof including serial and subterranean parts of the plant. It is contemplated that the parts of the plant may be for example, flowers, fruits or vegetables, shoots, leaves, needles, stalks, stems, fruiting bodies, seeds also roots and that parts of the plants may or may not be attached to the remainder of the plant.

The term “locus” of a plant as used herein is intended to embrace the place on which the plants are growing, where the plant propagation materials of the plants are sown or where the plant propagation materials of the plants will be placed into the soil.

The term “plant propagation material” is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil. These young plants may be protected before transplantation by a total or partial treatment by immersion.

It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.

The term “agrochemical” as used herein is understood to denote an agricultural chemical such as pesticides, fungicides, insecticides, acaricides, herbicides, nematicides, plant growth regulators and can be used interchangeably.

It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components, or groups thereof.

The term “mites” as used herein, includes small arachnids (eight-legged arthropods) of two large orders, the Acariformes and the Parasitiformes. These are tiny, less than 1 mm (0.04 in) in length, and have a simple, unsegmented body plan. These are significant agricultural pests that feed on plants, causing damage by sucking sap, inducing galls, transmitting diseases, and leading to yield loss.

The term “miticide” as used herein, refers to a compound that kills or materially inhibits the growth, proliferation, reproduction, or spread of mites.

The term “miticidal” as used herein, refers to the ability of a pesticide or pesticidal active to increase mortality or inhibit growth rate of mites.

The term “miticidally effective amount” refers to an amount of miticidal or pesticidal active compound or combination of such compounds capable of killing or materially inhibiting the growth, proliferation, reproduction or spread of mites.

The term “agrochemically acceptable excipients”, as used herein means excipients which are known and accepted in the art for formation of formulations for agricultural or horticultural use.

The expression “controlling mites” as used herein, means killing mites or preventing mites to develop, to grow, to proliferate or preventing mites to infect or infest, or inhibiting the rate and extent of mite attack, or delaying the onset of a mite attack.

The terms “weight percent”, “wt-%”, “percent by weight”, “% by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100. It is understood that, as used here, “percent “, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc.

Abbreviations:
DM Demineralised water
RTU Ready to use
QS Quantum satis
A.I/ a.i Active ingredient
Ai/ha Active ingredient per hectare
DAA Days after application
OD Oil Dispersion
MEC Microemulsion concentrate
PPM Parts per million
IPM Integrated Pest Management
ASS Accelerated Stability Studies

Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.

The active ingredients employed in the miticidal composition of the present invention ie. Hexythiazox and Etoxazole are individually known and available commercially as solo formulations.

Hexythiazox (IUPAC name: (4S,5S)-5-(4-chlorophenyl)-N-cyclohexyl-4-methyl-2-oxo-1,3-thiazolidine-3-carboxamide) is a non-systemic insecticide and acaricide (miticide) primarily used to control mites on various crops. It works by disrupting the normal development of mites, preventing them from reaching maturity and reproducing. It is effective against all stages of mites, including eggs, larvae, nymphs, and adults. Hexythiazox is a contact and stomach poison that inhibits mite growth, specifically targeting the molting process. It is effective against a wide range of mites, including red spider mites, yellow mites, and European red mites. It is used on various crops, such as tea, chilli, apple, grapes, and many fruits and vegetables. Its chemical structure is depicted below:

Etoxazole, IUPAC name (4-(4-tert-butyl-2-ethoxyphenyl)-2-(2,6-difluorophenyl)-4,5-dihydro-1,3-oxazole) is an acaricide (miticide/ovicide) belonging to the diphenyloxazoline group of chemicals. It controls mites through inhibition of chitin biosynthesis and by causing adults to lay sterile eggs. It is used in citrus, pome fruit, vegetables, and strawberries. It was discovered by Yashima Chemical Industry Co., Ltd. And developed jointly by Kyoyu and Sumitomo Chemical Co. Ltd. Its chemical structure is depicted below:

The Applicant submits that the present invention does not use any biological material originating in India. The vegetable oil as used in the presently claimed miticidal composition has been procured from 4825 Jalan Permatang Pauh, 13400 Butterworth, P.W. Malaysia.
In an embodiment, the miticidal composition of the present invention comprises a) Hexythiazox as the first active ingredient b) Etoxazole as the second active ingredient and c) at least one agrochemically acceptable excipient.

In another embodiment, the miticidal composition of the present invention comprises a) Hexythiazox, the first active ingredient in an amount ranging from 1-25% w/w b) Etoxazole, the second active ingredient in an amount ranging from 5-30% w/w and c) at least one agrochemically acceptable excipient.

In yet another embodiment, the miticidal composition of the present invention comprises a) Hexythiazox, the first active ingredient in an amount of 9% w/w b) Etoxazole in an amount of 12% w/w and c) at least one agrochemically acceptable excipient.

In another embodiment, the miticidal composition of the present invention provides a miticidal composition that delivers a miticidal action which is significantly enhanced as compared to that achieved through application of solo active formulations and in fact is greater than the sum of the miticidal action of the solo active formulations and hence possesses a synergistic effect.

In a preferred embodiment, the miticidal composition of the present invention is a ready -to- use composition or formulation. The other approaches for application of active combination is sequential application and applying a tank mix of solo formulations. Sequential application increases the chemical load in the soil and it is not an environmentally friendly approach. A tank mix is an unscientific practice adopted by the farmers which usually results in a heterogenous mixtures. Further, it has applicator and inhalation hazards and not considered an environmentally safe option.

A ready-to-use (RTU) composition or formulation on the other hand results in a homogenous composition which is easy to handle with lower chances of sedimentation, application hazard, phytotoxicity and are environmentally safer.

Thus, in an embodiment, the present invention provides a ready-to-use miticidal composition comprising a) Hexythiazox as the first active component b) Etoxazole as the second active component and c) at least one agrochemically acceptable excipient.

In a preferred embodiment, the present invention provides a novel, stable, synergistic, ready to use miticidal composition comprising a) Hexythiazox in an amount ranging from 1-25% w/w b) Etoxazole in an amount ranging from 5-30% w/w and at least one agrochemically acceptable excipient.

In most preferred embodiment, the present invention provides a novel, stable, synergistic miticidal composition comprising Hexythiazox in an amount of 9% w/w, Etoxazole in an amount of 12% w/w and at least one agrochemically acceptable excipient.

In a further embodiment, the miticidal composition of the present invention comprising said active ingredients when combined in specific weight percentage, exhibits synergistic effect in controlling mites, particularly Broad mite in Chilli and Tea Red Spider Mite.

In an embodiment, the miticidal composition of the present invention is suitable for applying to a variety of agricultural crops including but not limited to Tea, Chilli, Apple, Grapes, Rose, Brinjal Coffee, Capsicum and Okra.

In an embodiment, the miticidal composition of the present invention is highly effective in controlling mites including but not limited to Yellow Mite (Polyphagotarsonemus latus), Red spider mite (Tetranychus urticae), Tea Red Spider mite (Oligonychus coffeae), Pink mite (or) Orange mite (Acaphylla theae), Scarlet mite (Brevipalpus californicus) in variety of agricultural crops.

In an embodiment, the miticidal composition of the present invention further comprises at least one agrochemically acceptable excipient.

In an embodiment, at least one agrochemically acceptable excipient is selected from the group comprising an emulsifier, a surfactant, a co-surfactant, a stabilizer, a rheology modifier, a solvent, a co-solvent, anticaking agent, and demineralised water.

In another preferred embodiment, the agrochemically acceptable excipient is selected from the group comprising stabilizer, surfactant, emulsifier, solvent, co-solvent, rheology modifier, and demineralised water.

In another preferred embodiment, the miticidal composition of the present invention is formulated as a micro emulsion concentrate (MEC) comprising Hexythiazox technical, Etoxazole technical, at least a solvent, at least a co- solvent, at least a stabilizer and at least an emulsifier and demineralized water.

In an embodiment, the solvent is selected from the group comprising of normal butanol (NBA), Dimethyl sulfoxide (DMSO), N- methyl pyrrolidone (NMP), aromatic hydrocarbon solvents, cyclohexanone and and mixtures thereof. The solvent is present in an amount ranging from 10-40% w/w. In a preferred embodiment, the solvent is N- methyl pyrrolidone.

In an embodiment, the co-solvent is selected from the group comprising of Dimethyl sulfoxide (DMSO), soybean or palm oil methyl ester, benzyl alcohol, n-methylpyrrolidone (NMP), xylene, methanol, ethanol, acetophenone, 2-ethylhexyl acetate, cyclohexyl acetate, cyclohexanone, isopropanol, mineral oil, ester derived from lactic acid or mixture thereof. In an embodiment the co-solvent is present in an amount ranging from 1-10%w/w.

In an embodiment, the emulsifier is a non -ionic emulsifier selected from the group comprising of polyol esters, glycerol ethers, polyalkylene oxide block copolymer, oxyethylenated and/or oxypropylenated ethers, and ethylene glycol polymers.

In another embodiment, the emulsifier may include both a polyol ester or an ethylene glycol polymer, for example, glyceryl stearate and PEG-100 stearate. In an embodiment, the emulsifier is present in an amount ranging from 15-40% w/w.
In an embodiment, the stabilizer is selected from the group comprising of oils like vegetable oils, natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated shortchain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6-C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester. In a preferred embodiment, the stabilizer is vegetable oil.

In another embodiment, the vegetable oil is selected from the group comprising of olive oil, sunflower oil, canola oil, coconut oil, safflower oil, corn oil, peanut oil, cottonseed oil, palm-kernel oil, and soybean oil. In a preferred embodiment, the vegetable oil is soybean oil. In an embodiment, the stabilizer is present in an amount ranging from 1-8 %w/w.

In a preferred embodiment, the presently disclosed miticidal composition in the form of micro emulsion concentrate comprises Hexythiazox in an amount ranging from 1-25% w/w, Etoxazole in an amount ranging from 5-30% w/w, stabilizer in an amount ranging from 1-8% w/w, solvent in an amount ranging from 10-40%w/w, co-solvent in an amount ranging from 1-10% w/w, emulsifier in an amount ranging from 15-40% w/w and demineralized water in an amount ranging from 0-20% w/w.

In the most preferred embodiment, the presently disclosed miticidal Micro emulsion concentrate comprising Hexythiazox in an amount of 9% w/w, Etoxazole in an amount ranging from 12% w/w, stabilizer in an amount of 2% w/w, co-solvent in an amount of 5% w/w, solvent in an amount of 24% w/w, emulsifier in an amount of 38% w/w and demineralized water in an amount of 10% w/w. For ease of reference, said miticidal microemulsion concentrate is coded as CI-1209 MEC.

In another preferred embodiment, the miticidal composition of the present invention is developed in the form of oil dispersion (OD).

In an embodiment, the miticidal composition of the present invention in the form of oil dispersion comprises a rheology modifier, a surfactant, a stabilizer, an emulsifier, and a solvent.

In an embodiment, the miticidal oil dispersion composition of the present invention comprises a rheology modifier selected from the group comprising Aerosil 200 (hydrophilic fumed silica), silicon dioxide, magnesium alumina meta silicate, hydrophobic silica, colloidal silica, precipitated silica, hydrophobic silica powder, Syloid XDP, Syloid 244FP, microcrystalline cellulose PH 102 (MCC PH102) and Sipernat 22S, SYLOID 244 FP silica, Aerosil R812 and R805 a. In a preferred embodiment, the rheology modifier is Aerosil 200 (hydrophilic fumed silica). In an embodiment, the rheology modifier is present in an amount ranging from 0.5-5% w/w.

In an embodiment, the miticidal oil dispersion composition of the present invention comprises a stabilizer. In an embodiment, the stabilizer is selected from the group comprising vegetable oil ethoxylate; natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated short chain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6- C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester. In a preferred embodiment, the stabilizer is vegetable oil ethoxylate. In an embodiment, the stabilizer is present in an amount ranging from 10-30% w/w.

In an embodiment, the miticidal oil dispersion composition of the present invention comprises an emulsifier. In an embodiment, said emulsifier is selected from the group comprising vegetable oil ester alkoxylates, ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester. In a preferred embodiment, the emulsifier is vegetable oil ester alkoxylate. In an embodiment, the emulsifier is present in an amount ranging from 2-15% w/w.
In an embodiment, the miticidal composition of the present invention comprises a surfactant selected from the group comprising mono unsaturated fatty acids like Cis-9-octadecenoic acid, also known as oleic acid, polyoxyethylene alkyl ether, trisiloxane ethoxylate, polysorbates, ethoxylated tristyrylphenol phosphate, sodium lauryl sulphate or sodium methyl oleoyl taurate. In a preferred embodiment, the surfactant is Cis-9-octadecenoic acid, also known as oleic acid. In an embodiment, the surfactant is present in an amount ranging from 5-20% w/w.

In an embodiment, the miticidal composition of the present invention in the form of oil dispersion comprises a solvent. In a preferred embodiment, the solvent is selected from the group comprising methyl oleate, (fatty acid methyl ester derived from oleic acid), vegetable oils, methylated vegetable oils, oil medium selected from the group comprising, esterified fatty acids selected from methyl ester of triglycerides containing Cn -Cn saturated and unsaturated fatty acids, ethyl ester of triglycerides containing Cn -C22 saturated and unsaturated fatty acids such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester, bio diesels, paraffinic oil or tall oil. In a preferred embodiment, the solvent is oleic acid methyl ester. In an embodiment, the solvent is present in an amount ranging from 25-60% w/w.

In an embodiment, the miticidal oil dispersion of the present invention comprises a rheology modifier in an amount ranging from 0.5-5% w/w, a surfactant in an amount ranging from 5-20% w/w, a stabilizer in an amount ranging from 10-30% w/w, an emulsifier in an amount ranging from 2-15% w/w and a solvent in an amount ranging from 25-60% w/w.

In a preferred embodiment, the miticidal oil dispersion composition of the present invention comprises Etoxazole in an amount of 12% w/w, Hexythiazox in an amount of 9% w/w, rheology modifier in an amount of 1.5% w/w, surfactant in an amount of 9% w/w, stabilizer in an amount of 15% w/w, emulsifier in an amount of 5% w/w and solvent in an amount of 48.5% w/w. For ease of reference said miticidal oil dispersion is coded as CI-1209 OD.

In an embodiment, the miticidal composition of the present invention is formulated in a liquid formulation selected from the group comprising emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), aqueous suspo-emulsions (SE). In a preferred embodiment, the miticidal composition is in the form of an oil dispersion (OD) and Microemulsion concentrate (MEC).

In one embodiment, the miticidal composition comprising Etoxazole and Hexythiazox in an agrochemically acceptable formulation matrix, including but not limited to microemulsion (ME) or oil-dispersion (OD) types. When applied to a plant, a plant part, plant propagation material, or the locus thereof, in a miticidally effective manner, the composition controls mites at any developmental stage, including eggs, larvae, nymphs (e.g., protonymph, deutonymph, tritonymph), and adults.

In the most preferred embodiment, the miticidal composition of the present invention is in the form of a MEC (Micro emulsion concentrate). A microemulsion concentrate has the following advantages over other types of formulations:
Thermodynamically stable leading to increased shelf life
Low solvent level
Easy to transport and store.
High flash point and safe handling
Improved bioefficacy due to the solubilisation of active ingredient in the micro droplets
Low viscosity and easier to handle

EXAMPLES:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.

Example 1(a): Preparation of present miticidal composition as an Oil dispersion (OD)
In an embodiment, the chemical constitution of the present miticidal composition as an Oil dispersion (OD) is depicted below.

Table 1: Preferred composition of CI 1209 OD
S.no. Constituent Weight Percentage w/w
1. Etoxazole (100% basis) 12.0
2. Hexythiazox (100% basis) 9.0
3. Rheology modifier (Aerosil 200) 1.5
4. Surfactant (Cis-9-octadecanoic acid) 9.0
5. Stabilizer (vegetable oil ethoxylate) 15.0
6. Emulsifier (vegetable oil ester alkoxylate) 5.0
7. Solvent (Oleic acid methyl ester) 48.5
Total 100

Steps for preparation of Oil dispersion (OD):
Step 1: Required amount of solvent, stabilizer, surfactant, and emulsifier are taken and solubilized in a high shear mixer.
Step 2: Etoxazole and Hexythiazox actives are gradually added to the above mixture while under continuous stirring to make a homogenous mass.
Step 3: The mixtures obtained in step 2 is subjected to grinding in a sand mill. Grinding is carried out until a mean particle size of less than 12 microns is obtained.
Step 4: Post grinding, the material is mixed in a post mixer, and a suitable amount of rheology modifier is added.
Step 5: After mixing till a homogeneous mixture is obtained, quality parameters including pourability (to ensure that the OD can be poured from its container), dispersion stability, storage at elevated temperature are checked. If found compliant with quality parameters, desired packing or storage is done.

The chemical constitution of the present miticidal composition as a micro emulsion concentrate (MEC) is depicted below:

Example 1(b): Preparation of present miticidal composition as a Micro emulsion concentrate (MEC)

Table 2: Preferred composition of CI-1209 MEC
S.no. Constituent Weight Percentage w/w
1. Etoxazole (100% basis) 12.0
2. Hexythiazox (100% basis) 9.0
3. Stabilizer (Vegetable oil) 2.0
4. Co-solvent (Dimethyl sulfoxide (DMSO) 5.0
5. Emulsifier (Polyalkylene oxide block copolymer- Atlas G5002L) 38.0
6. DM water 10.0
7. Solvent (N-methyl pyrrolidone (NMP), 24.0
Total 100

Preparation method of Microemulsion concentrate (MEC):
Step 1: Required amount of solvent was taken and Etoxazole technical was added to make a solution.
Step 2: Hexythiazox technical was added gradually into the resulting solution
Step 3: Co-solvent was added to the solution and mixed well.
Step 4: Required amounts of Stabilizer and emulsifier are added to the solution followed by addition of demineralized water to get a clear solution.

Example 1(c): Accelerated storage stability test of CI 1209 MEC
The results obtained from accelerated storage stability test for various parameters are given below:

Table 3: Results of stability tests
Attributes Observation
S. No Parameters Specification Initial ASS@54°C±2°C for 14 Days
1. Description The material shall be in the form of clear homogeneous liquid free from any extraneous matter. Complies Complies
2. Etoxazole Content, % by mass 12.0 (±5%) 12.11 12.08
3. Hexythiazox Content, % by mass 9.0 (+10%, -5%) 9.15 9.12
4. pH (1% Aqueous Dispersion) 6.0-8.0 7.06 7.02
5. Persistent Foam after 1 Minute in ml Max. 60.0 32.0 40.0
6. Emulsion Stability@342 ppm Hard Water No Sediment & Separation After One Hour Complies Complies

Example 2: Field evaluation of the miticidal efficacy of the present miticidal composition

Example 2(a): Field evaluation in Target crop: Chilli

The presently disclosed miticidal composition was tested for its bioefficacy against Polyphagotarsonemus latus (broad mite) on a local variety in Chilli. The plants were aligned in a plot size of 50 sq. meter in a randomized block design. The trial was carried out in Guntur, Andhra Pradesh, during the kharif season 2024-2025 at a temp 25℃ and 74% relative humidity and windspeed of roughly 3.7 m/s. A total of 12 treatments were carried out in three replications. The application of the ready to use miticide composition was foliar (over the top).

The measured quantity of the chemical was added to the required volume of water at a rate of 375 L/ha. Initially, half of the required water volume was poured into the spray tank, followed by the addition of the chemical according to the specified dose. The remaining half of the water was then added, and the solution was stirred thoroughly to ensure proper mixing and complete coverage during spraying. Table 3 below provides the treatment details on on chilli (Capsicum annuum).

Table 3: Treatment details in Chilli
Treatment no. Treatment Dose a.i./ha (gm) Approx Dose Formulation (gm/ml/ha)
Untreated - -
T1 Etoxazole 10 % SC 40 400
T2 Hexythiazox (05.45 % w/w EC) 25 460
T3 CI-1209 OD
(Etoxazole 12% + Hexythiazox 9 % OD) 30 + 22.5 250
T4 CI-1209 OD
(Etoxazole 12% + Hexythiazox 9 % OD) 33 + 24.7 275
T5 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 30 + 22.5 250
T6 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 33 + 24.7 275

Each plot measured 5 × 5 meters, with chilli plants at a standard spacing. Recommended agronomic practices were followed throughout the study.

Evaluation of Bio efficacy:
The bio-efficacy evaluation involved counting the number of live mites on 10 tagged plants before application (pre-count) and at intervals of 1, 3, 5, 7 and 10 days after application (DAA), as well as at 5-day intervals thereafter until re-infestation. Observations included the count of live adults and nymphs from 30 leaves across the tagged plants. The percentage control of the live population was calculated using the formula based on the number of live sucking pests ie Mites (Polyphagotarsonemus latus) on chilli (Capsicum annuum).

Percent Control=(C-T)/C×100

Where C is No. of live Mite population in control plot
T is No. of live Mite population in treated plot
Data Collection:
Pest population assessment pre-treatment and post-treatment was carried out through pest counts/mite count recorded at 3, 7, and 10 days after treatment (DAT) from five randomly selected plants per plot. Crop parameters observations were recorded including mainly crop vigor and leaf damage which were taken to correlate pest suppression with plant health.

Statistical analysis
Analysis of Variance (ANOVA):
ANOVA was performed to compare the mean pest population, crop health parameters, and yield data across treatments. Significant differences among treatments were identified using the least significant difference (LSD) test at a 5% significance level (Gomez & Gomez, 1984).

Evaluation of synergy using Colby’s Method:
To determine interaction effects, the observed mortality rates of mites in two-way combinations were compared with expected mortality rates using Colby's formula (Colby, 1967).

The synergistic action expected for a given combination of two-way active components can be calculated as follows:
E=A+B-AB/100

Validation and Interpretation:
Results from ANOVA provided insights into treatment effectiveness, while Colby’s method elucidated the interactions among miticidal components. The combined results guided the evaluation of the performance of the novel formulation in pest suppression and yield enhancement.

Table 4: Synergistic effect of present composition against Broad Mite (Polyphagotarsonemus latus) in Chilli.
Treatment no. Treatment Dose a.i./ha (gm) Approx.Dose formulation (gm/ml/ha) % Reduction against control
(observed) % Reduction expected (Colby’s formula)
T1 Etoxazole 10 % SC 40 400 50.00 -
T2 Hexythiazox 05.45 % w/w EC 25 460 54.00 -
T3 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 30 + 22.5 250 87.00 77.00
T4 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 33 + 24.7 275 94.00 77.00
T5 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 30 + 22.5 250 89.00 77.00
T6 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 33 + 24.7 275 95.00 77.00

Calculation:
The synergistic action expected for the given two-way combination of active components for efficacy against Mites can be calculated as follows:
Expected (E)=77.00

Example 2(b) Field evaluation in Target crop: Tea
The presently disclosed miticidal composition was tested for its bioefficacy against tea red spider mite (Oligonychus coffeae) on a local variety of Tea (Camellia sinensis). The plants were aligned in a plot size of 50 sq. meter in a randomized block design. The trial was carried out in Barasat, West Bengal, during the kharif season 2024-2025 at a temp 25℃ and 74% relative humidity and windspeed of roughly 3.7 m/s. A total of 12 treatments were carried out in three replications. The application of the ready to use miticide composition was foliar (over the top).

Application method:
The measured quantity of the chemical was added to the required volume of water at a rate of 375 L/ha. Initially, half of the required water volume was poured into the spray tank, followed by the addition of the chemical according to the specified dose. The remaining half of the water was then added, and the solution was stirred thoroughly to ensure proper mixing and complete coverage during spraying.

Table 5: Treatment details in Tea crop
Treatment no. Treatment Dose a.i./ha (gm) Approx.
Dose Formulation (gm/ml/ha)
Untreated - -
T-1 Etoxazole 10 % SC 40 400
T-2 Hexythiazox 05.45 % w/w EC 25 460
T-3 Etoxazole 12% + Hexythiazox 9 % OD 30 + 22.5 250
T-4 Etoxazole 12% + Hexythiazox 9 % OD 33 + 24.7 275
T-5 Etoxazole 12% + Hexythiazox 9 % MEC 30 + 22.5 250
T-6 Etoxazole 12% + Hexythiazox 9 % MEC 33 + 24.7 275

Each plot measured 5 × 5 meters, with tea sown at a standard spacing. Recommended agronomic practices were followed throughout the study.

Evaluation of Bio efficacy:
The bio-efficacy evaluation involved counting the number of live Tea on 10 tagged plants before application (pre-count) and at intervals of 1, 3, 5, 7 and 10 days after application (DAA), as well as at 5-day intervals thereafter until re-infestation. Observations included the count of live adults and nymphs from 30 leaves across the tagged plants. The percentage control of the live population was calculated using the formula based on the number of live sucking pests Mite (Oligonychus coffeae) on Tea (Camellia sinensis).
Percent Control=(C-T)/C×100
Where C is No. of live Mite population in control plot
T is No. of live Mite population in treated plot

Data Collection:
Pest population assessment pre-treatment and post-treatment pest counts Mite were recorded at 3, 7, and 10 days after treatment (DAT) from five randomly selected plants per plot. Crop parameters observations on crop vigour and leaf damage were taken to correlate pest suppression with plant health. Yield data the final seed Tea yield was recorded to assess treatment impact.

Statistical analysis
Analysis of Variance (ANOVA):
ANOVA was performed to compare the mean pest population, crop health parameters, and yield data across treatments. Significant differences among treatments were identified using the least significant difference (LSD) test at a 5% significance level (Gomez & Gomez, 1984).

Colby’s Method for Synergism/Antagonism:
To determine interaction effects, the observed mortality rates of Mite (Oligonychus coffeae) in two- way combinations were compared with expected mortality rates using Colby's formula (Colby, 1967).
The synergistic action expected for a given combination of two-way active components can be calculated as follows:

E=A+B-AB/100
Validation and Interpretation:
Results from ANOVA provided insights into treatment effectiveness, while Colby’s method elucidated the interactions among miticidal components. The combined results guided the evaluation of the novel formulation's performance in pest suppression and yield enhancement.

Table 6: Synergistic miticidal action of present composition against Tea Red Spider Mite (Oligonychus coffeae)
Treatment no. Treatment Dose a.i./ha (gm) Approx. Dose Formulation (gm/ml/ha) % Reduction against control (Observed-Mite) % Reduction expected (Colby’s formula)
T1 Etoxazole 10 % SC 40 400 47.00 -
T2 Etoxazole 6% + Abamectin 1.5% SC 27 + 6.75 450 49.00 -
T3 Hexythiazox 05.45 % w/w EC 25 460 52.00 -
T4 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 30 + 22.5 250 87.00 74.56
T5 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 33 + 24.7 275 94.00 74.56
T6 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 30 + 22.5 250 89.00 74.56
T7 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 33 + 24.7 275 95.00 74.56

Calculation:
The synergistic action expected for a given combination of two-way active components can be calculated for Mite (Oligonychus coffeae) on Tea (Camellia sinensis):
Expected (E)=74.56

Example 3: Phytotoxicity observations and evaluation in target crops: Chilli and Tea

Phytotoxicity symptoms of mites, including leaf injury on tips and surfaces, were systematically scored, and rated using a standardized scale ranging from 0 to 10, where 0 indicated no visible symptoms and 10 represented severe damage.

Observations were recorded at 3, 7, and 10 days after application to evaluate potential phytotoxic effects. The parameters assessed included leaf injury on tips and surfaces, necrosis, vein clearing, epinasty, hyponasty, and wilting. Each parameter was rated using a standard phytotoxicity scale ranging from 0 to 10, where 0 indicated no damage and 10 represented severe damage as depicted in the Table below:

Table 7: Phytotoxicity symptoms Scoring and rating for leaf injury on tip/surface
Leaf injury on tips/surface 0% 1-10% 11-20% 21-30% 31-40% 41-50% 51-60% 61-70% 71-80% 81-90% 91-100%
Rating 0 1 2 3 4 5 6 7 8 9 10

During the evaluation at 1, 3, 5, 7, and 10 days after application, no phytotoxicity symptoms such as necrosis, vein clearing, epinasty, hyponasty, or wilting were observed on the treated plants. The absence of leaf injury and other adverse effects confirmed the safety of the tested miticidal combination, indicating its suitability for use without compromising the health and vigour of the target crop.

Table 8: Phytotoxicity evaluation of the present miticidal composition against Tea Red Spider Mite (Oligonychus coffeae)
Treatment no. Treatment Approx Dose a.i./ha (gm) Dose Formulation (gm/ml/ha) 1DAA 3 DAA 5
DAA 7 DAA 10
DAA
Untreated - - 0 0 0 0 0
T1 Etoxazole 10 % SC 40 400 0 0 0 0 0
T2 Hexythiazox 05.45 % w/w EC 25 460 0 0 0 0 0
T3 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 30 + 22.5 250 0 0 0 0 0
T4 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 33 + 24.7 275 0 0 0 0 0
T5 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 30 + 22.5 250 0 0 0 0 0
T6 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 33 + 24.7 275 0 0 0 0 0

Table 9: Phytotoxicity evaluation of present miticidal composition against Broad Mite (Polyphagotarsonemus latus) in Chilli

Treatment no. Treatment Dose a.i./ha (gm) Approx Dose Formulation (gm/ml/ha) 1
DAA 3 DAA 5
5
DAA 7 DAA 10
DAA
Untreated - - 0 0 0 0 0
T-1 Etoxazole 10 % SC 40 400 0 0 0 0 0
T-2 Hexythiazox 05.45 % w/w EC 25 460 0 0 0 0 0
T-3 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 30 + 22.5 250 0 0 0 0 0
T-4 CI-1209 OD (Etoxazole 12% + Hexythiazox 9 % OD) 33 + 24.7 275 0 0 0 0 0
T-5 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 30 + 22.5 250 0 0 0 0 0
T-6 CI-1209 MEC (Etoxazole 12% + Hexythiazox 9 % MEC) 33 + 24.7 275 0 0 0 0 0

Results and discussion:

The efficacy of the novel MEC (Microemulsion Concentrate) formulation of Etoxazole 12% + Hexythiazox 9% was comprehensively evaluated against the broad mite (Polyphagotarsonemus latus) in chilli (Capsicum annuum) and Tea Red Spider Mite (Oligonychus coffeae). This advanced formulation leverages the benefits of microemulsion technology to ensure better solubility, uniform distribution, improved leaf coverage, and enhanced penetration into plant tissues, which are critical for effective miticidal activity in dense tea canopies.

A rigorous field evaluation was conducted using robust statistical methods, including Analysis of Variance (ANOVA) and Colby’s synergy model, to determine the bio-efficacy and potential synergistic interactions of the combination. The study incorporated a two-tier dosing schedule, with the higher treatment at 33 + 24.7 g a.i./ha (Etoxazole + Hexythiazox), alongside the reduced dose of 30+22.5g ai/ha for comparative analysis.

The ANOVA results confirmed that the MEC formulation significantly suppressed mite populations across both the tested doses compared to the untreated control and the solo marketed formulations. The higher tested dose (33 + 24.7 g a.i./ha CI 1209 MEC), achieved an impressive 95% reduction in mite populations post-application in both the target crops Chilli and Tea, indicating superior control efficacy as compared to individual active formulations and marketed solo active formulations.

Further Importantly, Colby’s method revealed that the combination exhibited a synergistic interaction, with observed control levels exceeding the additive expectations of the individual active ingredients. This synergism between Hexythiazox, an ovicidal and larvicidal mite growth regulator, and Etoxazole, an inhibitor of mite chitin synthesis, is especially beneficial for breaking the mite reproductive cycle and achieving prolonged control with fewer applications.

The dual mode of action promised and shown by the present miticidal composition, ensures both immediate knockdown and residual protection, a key advantage in integrated pest management (IPM) programs. The synergy not only boosts efficacy but also opens opportunities for dose minimization, thus reducing chemical input, environmental footprint, and the risk of resistance development.

From a sustainability perspective, the MEC formulation of Etoxazole 12% + Hexythiazox 9% aligns with modern agricultural practices by combining high efficacy, crop safety, and environmental compatibility. Its ability to provide robust mite control with lower chemical loads supports the long-term goals of sustainable crop production through satisfactory mite control.

The Hexythiazox 9% + Etoxazole 12% MEC formulation represents a significant advancement in mite management strategies for control of mites, specifically broad mite in Chilli and Tea Red Spider Mite in tea. Its enhanced delivery through MEC technology, synergistic effect, and excellent safety profile make it an ideal choice for incorporation into IPM (integrated pest management programs). Adoption of this formulation can, not only maximize crop productivity and profitability but also contribute to sustainable, eco-friendly pest management practices. The present composition, therefore, stands out as a valuable, scientifically validated solution for effectively managing mite infestations under varying agro-climatic conditions.

While the invention is amenable to various modifications and alternative forms, some embodiments have been illustrated by way of example in the experiments and are described in detail above. The intention, however, is not to limit the invention by those examples and the invention is intended to cover all modifications, equivalents, and alternatives to the embodiments described in this specification.

The embodiments in the specification are described in a progressive manner and focus of description in each embodiment is the difference from other embodiments. For same or similar parts of each embodiment, reference may be made to each other.

It will be appreciated by those skilled in the art that the above description was in respect of preferred embodiments and that various alterations and modifications are possible within the broad scope of the appended claims without departing from the spirit of the invention with the necessary modifications.

Based on the description of disclosed embodiments, persons skilled in the art can implement or apply the present disclosure. Various modifications of the embodiments are apparent to persons skilled in the art, and general principles defined in the specification can be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure is not limited to the embodiments in the specification but intends to cover the most extensive scope consistent with the principle and the novel features disclosed in the specification.
, Claims:We Claim:
1. A miticidal composition comprising:
a) Hexythiazox in an amount ranging from 1-25% w/w,
b) Etoxazole in an amount ranging from 5-30% w/w, and
c) at least one agrochemically acceptable excipient.

2. The miticidal composition as claimed in claim 1, wherein the agrochemically acceptable excipient is selected from the group comprising of stabilizer, surfactant, co-surfactant, emulsifier, solvent, co-solvent, rheology modifier, anticaking agent, and demineralised water.

3. The miticidal composition as claimed in claim 2, wherein the solvent is selected from a group comprising of normal butanol (NBA), Dimethyl sulfoxide (DMSO), N- methyl pyrrolidone (NMP), aromatic hydrocarbon solvents, and cyclohexanone.

4. The miticidal composition as claimed in claim 2, wherein the co solvent is selected from the group comprising of Dimethyl sulfoxide (DMSO), soybean or palm oil methyl ester, benzyl alcohol, N-Methylpyrrolidone (NMP), xylene, methanol, ethanol, acetophenone, 2-ethylhexyl acetate, cyclohexyl acetate, cyclohexanone, isopropanol, mineral oil, ester derived from lactic acid.

5. The miticidal composition as claimed in claim 2, wherein the emulsifier is selected from the group comprising of polyol esters, glycerol ethers, oxyethylenated and/or oxypropylenated ethers, and ethylene glycol polymers.

6. The miticidal composition as claimed in claim 2, wherein the stabiliser is selected from the group comprising of vegetable oils, natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated shortchain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6-C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters.

7. The miticidal composition as claimed in claim 2, wherein the composition is formulated as emulsifiable concentrates (EC), emulsions (EW), micro-emulsion concentrates (MEC), suspension concentrates (SC), oil- dispersion (OD), flowable suspensions (FS), aqueous suspo-emulsions (SE).

8. The miticidal composition as claimed in claim 2, wherein the composition is formulated as micro emulsion concentrate.

9. The miticidal composition as claimed in claim 8, comprising:
Hexythiazox in an amount ranging from 1-25% w/w,
Etoxazole in an amount ranging from 5-30% w/w,
stabilizer in an amount ranging from 1-8% w/w,
solvent in an amount ranging from 10-40%w/w,
co-solvent in an amount ranging from 1-10% w/w,
emulsifier in an amount ranging from 15-40% w/w, and
demineralized water in an amount ranging from 0-20% w/w.

10. The miticidal composition as claimed in claim 2, wherein the miticidal composition is formulated as oil dispersion.

11. The miticidal composition as claimed in claim 10, comprising:
Hexythiazox in an amount ranging from 1-25% w/w;
Etoxazole in an amount ranging from 5-30% w/w;
rheology modifier in an amount ranging from 0.5-5% w/w,
surfactant in an amount ranging from 5-20% w/w;
stabilizer in an amount ranging from 10 -30% w/w,
emulsifier in an amount ranging from 2-15% w/w, and
solvent in an amount ranging from 25-60% w/w
12. The miticidal composition as claimed in claim 11, wherein said rheology modifier is selected from the group comprising hydrophilic fumed silica), silicon dioxide, magnesium alumina meta silicate, hydrophobic silica, colloidal silica, precipitated silica, hydrophobic silica powder and mixtures thereof.

13. The miticidal composition as claimed in claim 11, wherein said surfactant is selected from the group comprising monounsaturated fatty acids (cis-9-octadecanoic acid), polyoxyethylene alkyl ether, trisiloxane ethoxylate, polysorbates, ethoxylated tristyrylphenol phosphate, sodium lauryl sulphate and sodium methyl oleoyl taurate.

14. The miticidal composition as claimed in claim 11, wherein said stabilizer is selected from the group comprising vegetable oil ethoxylate; natural or synthetic ethoxylated fatty alcohols, natural or synthetic, ethoxylated and propoxylated fatty alcohols, ethoxylated and propoxylated short chain alcohols, C6-C20 linear and branched alcohol ethoxylates, C6-C20 alcohol propoxylates, C6- C20 ethoxylated alcohols, C6-C20 propoxylated alcohols; ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester.

15. The miticidal composition as claimed in claim 11, wherein said emulsifier is selected from the group comprising vegetable oil ester alkoxylates, ethoxylated fatty acids, ethoxylated castor oil, ethoxylated sorbitan esters, ethoxylated esterified sorbitols, ethoxylated alkylphenols, ethoxylated tristyrylphenols and ethoxylated fatty amines, polyoxyethylene fatty acid esters such as polyoxyethylene monolaurate, polyoxyethylene distearate or polyoxyethylene resin acid ester.

16. The miticidal composition as claimed in claim 11, wherein said solvent is selected from the group comprising methyl oleate, (fatty acid methyl ester derived from oleic acid), vegetable oils, methylated vegetable oils, oil medium selected from the group comprising, esterified fatty acids selected from methyl ester of triglycerides containing Cn -Cn saturated and unsaturated fatty acids, ethyl ester of triglycerides containing Cn-C22 saturated and unsaturated fatty acids such as methyl soyate, ethyl soyate, rapeseed methyl ester, rapeseed ethyl ester, bio diesels, paraffinic oil or tall oil.

17. A method of preparing the miticidal micro emulsion concentrate as claimed in claims 9, the method comprising:
dissolving Etoxazole technical in an amount ranging from 5-30% w/w in a solvent in an amount of 10 to 40% w/w to obtain a first solution;
adding Hexythiazox technical in an amount ranging from 1-25% w/w into the first solution to obtain a second solution;
adding and mixing a co-solvent in an amount ranging from 1-10% w/w to the second solution;
subsequently adding stabiliser in an amount ranging from 1-8% w/w and an emulsifier in an amount ranging from 15-40% w/w, and
thereafter adding demineralized water to obtain a microemulsion concentrate.

18. A method of preparing the miticidal oil dispersion as claimed in claim 11, the method comprising:
adding and mixing stabilizer in an amount ranging from 10-30% w/w, surfactant in an amount ranging from 5-20% w/w, and an emulsifier in an amount ranging from 2-15% w/w, in a solvent in an amount of 25-60% w/w to obtain a mixture;
adding Etoxazole technical in an amount ranging from 5-30% w/w and Hexythiazox technical in an amount ranging from 1-25% w/w, to the mixture under continuous stirring to obtain a homogeneous mixture;
grinding the mixture to obtain a mean particle size of less than 12 microns; and
mixing and adding a rheology modifier in an amount ranging from 0.5-5% w/w to obtain an oil dispersion.

19. A method of improving plant growth by applying a miticidally effective amount of the miticidal composition as claimed in claims 1-16 on a plant or a part thereof or at the locus or on the surface of plant propagation material.