Description:FORM 2
THE PATENTS ACT, 1970
(39 OF 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10; rule 13)

1.TITLE OF THE INVENTION – AN ULTRA-HIGH INOCULUM POTENTIAL MYCORRHIZAL BIOFERTILIZER AND PROCESS OF PREPARATION THEREOF
2. APPLICANT(S)
(a) NAME: Curative Microbes Pvt. Ltd.
(b) NATIONALITY: INDIAN
(c) ADDRESS: Survey No.- 243, Makhiyala-Galiyavad road, At.- Makhiyala-362011, Ta. & Di.- Junagadh
3. PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the invention and the manner in
which it is to be performed.

AN ULTRA-HIGH INOCULUM POTENTIAL MYCORRHIZAL BIOFERTILIZER AND PROCESS OF PREPARATION THEREOF

FIELD OF THE INVENTION
The present invention relates to the field of compositions and methods for the developing of biofertilizers that are of organic origin. More particularly, the present invention relates to an ultra-high inoculum potential mycorrhizal biofertilizer. The present invention, thus relates to an ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of mycorrhizal fungi, a combination of carrier, a combination of nutrient additives, protective coating agents, moisture retainers and a solvent.

BACKGROUND OF THE INVENTION
From a last few decades it has been witnessed a large amount of activity in the general field of fertilizers and biofertilizers in particular have attracted special attention, primarily on account of them having been derived from natural sources and having an essentially biological origin and their possible exploitation in the numerous fields.

It is very well known that agriculture is the practice of cultivating land to grow crops or raising various livestocks. Agriculture is a key factor showing development of human civilization. As the human population is increasing the need for agricultural product is also increasing. Increasing global population necessitates a corresponding increase in the food production through agricultural intensification and expansion. Increased need for food production has increased the use of fertilizers, pesticides and insecticides to get an increased food production. But, some products are harmful for the soil and the land. Thus, there is a need for products that are less harmful to the soil and land.

Biofertilizers are natural fertilizers that contain living microorganisms, which enhance the nutrient availability and uptake for plants by colonizing the rhizosphere or the interior of the plant. They generally include bacteria, fungi and algae that improves soil fertility. Biofertilizers reduces the need for chemical fertilizers, promoting sustainable agriculture.

Mycorrhiza is a mutually benefical relationship between a green plant and a fungus. The green plant produces organic compounds like sugars through photosynthesis and provides them to the fungus, while the fungus supplies the green plant with water and essential mineral nutrients, such as phosphorous, extracted from the soil.

Mychorrizal biofertilizers are generally products that comprises of mycorrhizal fungi that forms a symbiotic relationships with plant roots, that enhances nutrients and water absorption in particularity absorption of phosphorous. This symbiotic relationship and association promotes growth of plant, improves and maintains soil fertility and is a sustainable approach to agriculture, reducing the need for chemical fertilizer.

Current mycorrhizal biofertilizers typically contain up to 10,000 inoculum potential (IP) per gram commercial products like DYNOMYCO offering 900 IP/g and research-based formulations exceeding 10,000 IP/g. High-IP Mychorrizal biofertilizers production by Root Organ Culture method, optimizing environmental conditions for better spore density and viability. Regulatory standards, such as in the Philippines (PNS/BAFS 183:2020), require 2,300 IP/g for root-based inoculants.

WO2013098829 document discloses a mycorrhizae based biofertilizer compositions, wherein the said fungus is from Glomus, Gigaspora and Scutellospora genera, in the form of a pre-maintained starter culture. These are then subjected to subculturing followed by incubation for attaining the desired growth. At the designated point when the desired growth has been achieved, the procedure for harvesting is put into effect. The harvested cultures are then subjected to sieving and air drying followed by blending to generate the various conceivable combinations for blended inoculum. This product and process is completely different from the present invention.

The available mycorrhizal biofertilizers generally lack of high inoculum potential, that results in less effective colonization of mycorrhizal inoculant with plant roots that reduces the plant growth, decreases the quality of soil and land. Thus, there is a constant need of developing an ultra-high inoculum potential biofertilizers that are beneficial for the plant and as well as soil and land.

The present invention is directed toward overcoming one or more of the problems discussed above.

OBJECTIVE OF THE PRESENT INVENTION
The main objective of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer.

Another objective of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer that improves nutrient uptake by plants, reducing chemical fertilizer dependency.

Another objective of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer that provides superior stress tolerance, boosting drought tolerance, salinity resistance and disease suppression.

Another objective of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer having better adaptability for farmers.

Another objective of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer that is easily scalable and is having cost-effective production.

Another objective of the present invention is to provide an ulta-high inoclum potential mycorrhizal biofertilizer that supports organic farming and contributes to sustainable agriculture.

SUMMARY OF THE INVENTION
The main aspect of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer.

Another aspect of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer, comprising of mycorrhizal fungi, a combination of carrier, a combination of nutrient additives, protective coating agents, moisture retainers and a solvent.

Another aspect of the present invention is to provide an ultra-high inoculum potential mycorrhizal biofertilizer and the process of preparing thereof.

DESCRIPTION OF THE INVENTION
The detailed description set forth below is intended as a description of exemplary embodiments and is not intended to represent the only forms in which the exemplary embodiments may be constructed and/or utilized. The description sets forth the functions and the sequence of steps for constructing and/or operating the exemplary embodiments. However, it is to be understood that the same or equivalent functions and sequences which may be accomplished by different exemplary methods are also intended to be encompassed within the spirit and scope of the invention.

As defined herein, all scientific and technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs.

Although any process and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described.

As stated in the present invention herein, the singular forms “a,” “an” and “the” specifically also encompass the plural forms of the terms to which they refer, unless the content clearly dictates otherwise.The term “about” is used herein to means approximately, in the region of, roughly, or around.

As stated herein, that it follows in a transitional phrase or in the body of a claim, the terms “comprise(s)” and “comprising” are to be interpreted as having an open-ended meaning. That is, the terms are to be interpreted synonymously with the phrases “having at least” or “including at least”. When used in the context of a process, the term “comprising” means that the process includes at least the recited steps but may include additional steps. When used in the context of a composition, the term “comprising” means that the composition includes at least the recited features or components but may also include additional features or components.

Nothing in this disclosure is to be construed as an admission that the embodiments described in this disclosure are not entitled to antedate such disclosure by virtue of prior invention. As used in this document, the term "comprising" means "including, but not limited to.

The term used herein, “mycorrhizal” refers to the symbiotic relationship between a fungus and the roots of a plant.

The term used herein, “inoculum potential” refers to to the ability of a pathogen or symbiotic organism (like mycorrhizal fungi) to colonize or infect a host. It’s basically a measure of how likely and how strongly an organism can establish itself in a new environment, especially in the roots of a plant.

The term used herein, “biofertilizer” refers to a environment friendly alternatives to chemical fertilizers that comprises of microorganisms, such as bacteria, fungi and algae.

The main embodiment of the present invention, is to provide an ultra-high inoculum potential mycorrhizal biofertilizer.

According to another embodiment of the present invention, the said ultra-high inoculum potential mycorrhizal biofertilizer comprises of mycorrhizal fungi, a combination of carrier, a combination of nutrient additives, protective coating agents, moisture retainers and a solvent.

According to another embodiment of the present invention, wherein said mycorrhizal fungi is selected from Rhizophagus intraradices, Rhizophagus clarus, Funneliformis mosseae, Claorideoglomus etunicatum, Acaulospora laevis, Gigaspora margarita, Scutellospora calospora and Diversispora versiformis or a combination thereof.

According to another embodiment of the present invention, wherein the preferred mycorrhizal fungi is a combination of Rhizophagus intraradices and Rhizophagus clarus.

According to another embodiment of the present invention, said mycorrhizal fungi is used in the range of 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

According to another embodiment of the present invention, the said carrier is selected from Bentonite, Lignite, Peat Moss, Vermiculite, Charcoal, Kaolin, Press Mud and Talc or a combination thereof.

According to another embodiment of the present invention, the preferred carrier is Bentonite.

According to another embodiment of the present invention, wherein said carrier is used in the range of 1 kg to 150 kg, more preferably 10 kg to 100 kg and most preferably 30 kg to 70 kg.

As per another embodiment, the said nutrient additives can be selected from amino acids, vitamins, growth factors, calcium carbonate, humic acid, glycerol, trehalose, mannitol and sucrose or a combination thereof.

According to another embodiment, the preferred nutrient additives are amino acids.

According to another embodiment, the preferred amino acids are Proline and Glutamic acid.

As per another embodiment, the said nutrient additives are used in the range of 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

As per the another embodiment, the protective coating agent is selected from Carboxymethyl cellulose (CMC), Sodium alginate, Gum arabic/Acacia Gum, Polyvinyl alcohol, Gelatin and starch-based gels.

As per another embodiment of the present invention, the preferred protective coating agent is Sodium Alginate.

As per another embodiment, the said protective coating agent is used in the 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

As per another emnbodiment, the starch-based binder is selected from Tapioca starch (obtained from cassava), corn starch, Potato starch and rice starch.

As per another embodiment, the said starch-based binder is 1 to 50 kg, more preferably 5 to 35 kg and most preferably 10 kg to 20 kg.

As per another embodiment of the present invention, water is used as a solvent.

As per another embodiment, the process for preparing the the ultra-high potential mycorrhizal biofertilizer comprises following steps:
a. Isolation and screening of high-efficiency fungal species;
b. Propagation of selected fungi from step (a) using the Root Organ Culture method to ensure enhanced spore production;
c. Mixing carrier materials in the optimized fungal inoculum of step (b) for better fungal adherence and stability;
d. Enhancing spore density of the mixture of step (c) to achieve 25,000–1,00,000 IP/g;
e. Drying the formulation of step (d) for retaining the viability;
f. Adding protective agents to step (e) for extending shelf life;
g. Controlling the viability and controlling contamination in the final product of step (f).

As per another embodiment, the ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of 0.1 to 50 kg of mycorrhizal fungi, 1 to 150 kg carrier, 0.1 to 50 kg nutrient additives, 0.1 to 50 kg of protective coating agent and 1 to 50 kg starch-based binders and a solvent.

As per another embodiment, the ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of 0.5 to 30 kg of mycorrhizal fungi, 10 to 100 kg carrier, 0.5 to 30 kg nutrient additives, 0.5 to 30 kg of protective coating agent and 5 to 35 kg starch-based binders and a solvent.

As per another embodiment, the ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of 1 to 10 kg of mycorrhizal fungi, 30 to 70 kg carrier, 1 to 10 kg nutrient additives, 1 to 10 kg of protective coating agent and 10 to 20 kg starch-based binders and a solvent.

As per another embodiment, the ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of 5 kg of Rhizophagus intraradices and Rhizophagus clarus as mycorrhizal biofertilizer, 65 kg of Bentonite as a carrier, 5 kg of amino acids blend as nutrient additives, 5 kg of sodium alginate as a protective coating agent, 15 kg of Tapioca starch as starch based binder and 3 liters of water as a solvent.

As per another embodiment of the present invention, wherein the said ultra-high inoculum potential of the biofertilizer is 25,000-1,00,000 inoculum potential per gram.

The invention is further illustrated by the following examples which are provided to be exemplary of the invention and do not limit the scope of the invention. While the present invention has been described in terms of its specific embodiments, certain modifications and equivalents will be apparent to those skilled in the art and are intended to be included within the scope of the present invention.

EXAMPLES
The following examples are provided for illustrative purposes only and are not intended to limit the scope of the invention.

EXAMPLE 1: TRIALS OF THE ULTRA-HIGH POTENTIAL BIOFERTILIZER
A study was carried out to optimize the composition of the biofertilizer with different ingredients and composition to produce the maximum inoculum potential. We formulated 4 batches with different composition and quantity. The trial batches are as below:

Batch T1:
Sr. No. Ingredient Quantity Purpose
1. Glomus intraradices spores 2 kg Core inoculum
2. Peat (Carrier) 45 kg Soil structure enhancer and organic carbon source
3. Bentonite (Carrier) 45 kg Moisture retention and buffering agent
4. CMC (Coating Agent) 5 kg Ensures spores adhere to the carrier
5. Water (Moisture Control) 3 liters Enhances microbial activity during mixing

Batch T2:
Sr. No. Ingredient Quantity Purpose
1. Rhizophagus irregularis spores 3 kg Core inoculum
2. Vermiculite (Carrier) 40 kg Improves aeration and moisture balance
3. CMC (Coating Agent) 5 kg Ensures spore attachment
4. Peat (Soil Enhancer) 30 kg Organic carbon source
5. Water (Moisture Control) 3 liters Enhances microbial activity during mixing

Batch T3:
Sr. No. Ingredient Quantity Purpose
1. Glomus intraradices spores 2.5 kg Core inoculum
2. Rhizophagus irregularis spores 2.5 kg Core inoculum
3. Zeolite (Carrier) 60 kg Provides mineral support and improves nutrient exchange
4. CMC (Coating Agent) 5 kg Ensures spores adhere to the carrier
5. Amino Acid Nutrient Blend 5 kg Boosts fungal growth
6. Water (Moisture Control) 3 liters Ensures even distribution and spore activation

Batch T4:
Sr. No. Ingredient Quantity Purpose
1. High-potency mycorrhizal spores 5 kg Core inoculum
2. Bentonite (Carrier) 65 kg Provides stable structure and water retention
3. Polymer (Coating Agent) 5 kg Enhances spore protection and longevity
4. Amino Acid Nutrient Blend 5 kg Promotes rapid fungal colonization
5. Sodium Alginate (Protective Coating Agent) 2 kg Ensures extended viability
6. Starch-based Binder 15 kg Maintains hydration and spore activity
7. Water (Moisture Control) 3 liters Ensures smooth blending and spore activation
EXAMPLE 2: RESULTS OF THE TRIAL BATCHES
The below table provides the results for the above four batches to optimize the formulation of the ultra-high inoculum potential biofertilizer.

Batch No. Mycorrhizal Strain Carrier Material IP Count (per gram) Viability (%) Root Colonization (%) Plant Growth Increase (%)
T1 Glomus intraradices Peat + Bentonite 8,000 70 45 12
T2 Rhizophagus irregularis Vermiculite + CMC 15,000 78 60 20
T3 (Optimized) G. intraradices + R. irregularis Zeolite + CMC 50,000 85 85 35
T4 (Final) High-Potency Mycorrhiza Bentonite + Polymer 1,00,000 90 92 45
Table 1: Results of the four trial batches

The above results showed that the Batch T4 showed the highest inoculum potential, root colonization and plant growth increase of the biofertilizer. Thus, the composition of batch T4 was finalized as the optimized batch of the biofertilizer.

EXAMPLE 3: OPTIMIZED BATCH OF THE ULTRA-HIGH INOCULUM POTENTIAL BIOFERTILIZER
Below is the optimized batch of the ultra-high inoculum potential biofertilizer:
Sr. No. Ingredient Quantity
1. High-potency mycorrhizal spores 5 kg
2. Bentonite (Carrier) 65 kg
3. Polymer (Coating Agent) 5 kg
4. Amino Acid Nutrient Blend 5 kg
5. Sodium Alginate (Protective Coating Agent) 2 kg
6. Starch-based Binder 15 kg
7. Water (Moisture Control) 3 liters
Table 2: Optimized formulation of the ultra-high inoculum potential biofertilizer

EXAMPLE 4: COMPARATIVE STUDY WITH THE MARKETED PRODUCTS
The below is a comparative study of marketed products with the optimized formulated ultra-high inoculum potential biofertilizer. Below table provides details regarding the marketed products used for comparative study.
Product Trade Name Company Name
Market Product A Dynomyco Mycorrhizal Inoculant Groundwork BioAg.
Market Product B VAMoz-P Utkarsh Aegrochem Pvt. Ltd.
Market Product C r-jaal Gold Biowall Agrihealth Pvt. Ltd.
Trade name and company name details of market products are listed below the table

Product IP Count (per gram) Root Colonization (%) Nutrient Uptake Increase (%) Plant Growth Improvement (%)
Market Product A 900 IP/g 30 10 12
Market Product B 5,000 IP/g 55 18 22
Market Product C 10,000 IP/g 65 22 28
New Formulation 100,000 IP/g 92 45 55
Table 3: Comparative study of the present formulation with marketed product
Moreover, various other parameters were studied with the marketed products in comparison with the ultra-high inoculum potential biofertilizer.
Parameter Control (No Mycorrhiza) Market Product A Market Product B Market Product C New Mycorrhiza Formulation
Soil Organic Matter (%) 1.2 1.8 2.4 3.0 3.0
Phosphorus Availability (%) 12 18 25 35 35
Plant Disease Reduction (%) 10 20 30 45 45
Crop Yield Increase (%) 0 10 22 35 50
Farmer Cost Savings (INR per Acre) 0 1,500 3,000 4,000 4,500
Table 4: Comparative study of various parameters the present formulation with marketed products

Factor Control (No Mycorrhiza) Market Product A Market Product B Market Product C New Mycorrhiza Formulation
Cost Per Acre (INR) 2,500 2,000 2,800 3,200 1,500
Reduction in Chemical Fertilizer Use (%) 0 10 25 30 50
Yield Increase (%) 0 10 22 28 50
ROI for Farmers 1:1 03:01 04:01 5:1 07:01
Table 5: Comparative study of the present formulation with marketed products

EXAMPLE 5: SYNERGISTIC STUDY WITH THE OTHER BIOFERTILIZERS
A synergistic study with the other biofertilizers was carried out to understand the interaction of different microbial formulations interaction and enhancement of the biofertizer activity. Below table provides the data for the same.
Combination Root Colonization (%) Nutrient Uptake (%) Plant Growth (%) Plant Growth Increase (%)
Mycorrhiza Alone 85 35 25 45
Mycorrhiza+PGPR (Pseudomonas) 88 40 30 50
Mycorrhiza+Trichoderma 90 42 33 52
High-Potency Mycorrhiza + PGPR + Trichoderma 95 50 45 59
Table 6: Synergistic study of the present formulation with other biofertilizers

The studies indicates that the present ultra-high inoculum has enhanced nutrient mobalization, improved plant growth and yield, balanced soil microbiome, stress tolerance enhancement and cost efficiency for farmers.

Moreover, it was also observed that when farmers use other biofertilizers along with our new mycorrihza formulation, no negative effects were observed. This confirms the compatibility and safety of integrating our product with commonly used biofertilizers in agricultural practices.

EXAMPLE 6: OPTIMIZATION OF GROWTH CONDITIONS
The optimized batch was subjected to optimize the growth conditions as follows:
Parameter Trial 1 Trial 2 Trial 3 (Optimized Process)
Temperature (°C) 25 28 30
pH Level 5.5 6.2 6.8
Moisture (%) 40 50 55
Aeration Rate (L/min) 2 2.5 3
Substrate Type Peat Vermiculite Bentonite + Polymer
Table 7: Optimized growth conditions of the present formulation

The above results showed that Trial 3 showed optimized growth conditions for manufacturing the ultra-high inoculum potential biofertilizers.

EXAMPLE 7:STABILITY AND SHELF-LIFE TESTING
The optimized batch and formulation was subjected to stability and shelf-life testing at three different temperatures and results were carried out for 3 to 12 months.
Storage Condition Viability After 3 Months (%) Viability After 6 Months (%) Viability After 12 Months (%)
Room Temperature (25°C) 85 70 45
Refrigerated (4°C) 95 90 80
High Temp (40°C) 65 40 20
Table 8: Stability study of the present formulation

The above studies provide us that the present ultra-high inoculum potential biofertilizer has an ultra-high inoculum potential, is stable, provides increased plant growth and yield, superior stress tolerance, eco-friendly and sustainable, is cost-effective for farmers, faster colonization and effectiveness, scalable and efficient production, sustained efficacy across growth stages and maintains or restores soil fertility.
, Claims:CLAIMS
We Claim,
1. An ultra-high inoculum potential mycorrhizal biofertilizer, wherein the biofertilizer comprises of mycorrhizal fungi, a combination of carrier, a combination of nutrient additives, protective coating agents, moisture retainers and a solvent.

2. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said mycorrhizal fungi is selected from Rhizophagus intraradices, Rhizophagus clarus, Funneliformis mosseae, Claorideoglomus etunicatum, Acaulospora laevis, Gigaspora margarita, Scutellospora calospora and Diversispora versiformis or a combination thereof.

3. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said mycorrhizal fungi is used in the range of 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

4. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said carrier is selected from Bentonite, Lignite, Peat Moss, Vermiculite, Charcoal, Kaolin, Press Mud and Talc or a combination thereof.

5. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said carrier is used in the range of 1 kg to 150 kg, more preferably 10 kg to 100 kg and most preferably 30 kg to 70 kg.

6. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said nutrient additive is selected from amino acids, vitamins, growth factors, calcium carbonate, humic acid, glycerol, trehalose, mannitol and sucrose.
7. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said nutritive additive is used in the range of 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

8. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said the protective coating agent is selected from Carboxymethyl cellulose (CMC), Sodium alginate, Gum arabic/Acacia Gum, Polyvinyl alcohol, Gelatin and starch-based gels.

9. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said protective coating agent is used in the 0.1 to 50 kg, more preferably 0.5 to 30 kg and most preferably 1 kg to 10 kg.

10. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein the starch-based binder is selected from Tapioca starch, corn starch, Potato starch and rice starch.

11. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein the starch-based binder is 1 to 50 kg, more preferably 5 to 35 kg and most preferably 10 kg to 20 kg.

12. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein the process for preparing the ultra-high potential mycorrhizal biofertilizer comprises following steps:
a. Isolation and screening of high-efficiency fungal species;
b. Propagation of selected fungi from step (a) using the Root Organ Culture method to ensure enhanced spore production;
c. Mixing carrier materials in the optimized fungal inoculum of step (b) for better fungal adherence and stability;
d. Enhancing spore density of the mixture of step (c) to achieve 25,000–1,00,000 IP/g;
e. Drying the formulation of step (d) for retaining the viability;
f. Adding protective agents to step (e) for extending shelf life;
g. Controlling the viability and controlling contamination in the final product of step (f).

13. The ultra-high inoculum potential mycorrhizal biofertilizer as claimed in claim 1, wherein said biofertilizer has 25,000-1,00,000 inoculum potential per gram.


Dated this 20th May, 2025