Description:FIELD OF THE INVENTION

[0001] The present invention relates to a bacteria culturing device that is capable of automatically preparing, maintaining, and monitoring optimal conditions for bacterial cultivation.

BACKGROUND OF THE INVENTION

[0002] Bacteria are cultured to study their characteristics, reproduce them for research, develop antibiotics, test drug effectiveness, and produce useful compounds like enzymes, vaccines, or probiotics. Culturing allows scientists to observe bacterial behavior, genetics, and interactions under controlled conditions, which supports advancements in medicine, biotechnology, food safety, and environmental monitoring. Bacteria culturing faces challenges such as contamination from external microbes, inconsistent environmental conditions, difficulty maintaining sterility, uneven nutrient distribution, and inaccurate monitoring of growth. These issues affect the reliability and reproducibility of results, leading to poor bacterial yield, altered behavior, or compromised experimental outcomes in research and industrial applications.

[0003] Traditionally, bacteria culturing is performed using manual devices such as Petri dishes, incubators, and laminar flow hoods. These setups require human intervention for tasks like media preparation, inoculation, environmental monitoring, and growth analysis. The lack of automation in such systems leads to several problems, including inconsistent handling, increased risk of contamination, limited scalability, and high labor dependency. Environmental conditions like temperature, humidity, and nutrient delivery are often controlled manually, resulting in fluctuations that affect bacterial growth. Moreover, traditional devices typically lack real-time monitoring and data integration capabilities, making it difficult to achieve precision, repeatability, and efficiency in bacterial culturing processes.

[0004] US3856627A discloses about a culture medium containing fish protein hydrolysate which is prepared by hydrolyzing fish protein with protease. This culture medium can be used as a substitute for conventional culture media containing animal organ extracts for bacterial cultivation because of its superior growth enhancing effects, uniformity in quality, convenience and low price.

[0005] CA2035728C discloses about an instrument and a sealable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium communicating with a sensor means containing an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO2 concentration in the medium are detected from outside the vessel.

[0006] Conventionally, many devices are available in market for assisting in bacteria culturing. However, these devices lack in automation, precision, and integration of multiple culturing functions into a single platform. Most traditional devices require manual operations for media handling, inoculation, environmental control, and observation, which increases the risk of contamination and human error. Furthermore, they often do not support dynamic adjustment of culturing conditions such as temperature, humidity, oxygen levels, and nutrient supply, which are critical for optimized bacterial growth. The absence of real-time monitoring, data processing, and autonomous feedbacks further limits their effectiveness in research or industrial settings.

[0007] In order to overcome the aforementioned drawbacks, there exists a need in the art to develop a device that offers an integrated, automated solution for bacterial culturing. The device further minimizes human intervention while ensuring precise control over critical environmental parameters such as temperature, humidity, oxygen concentration, and nutrient delivery. The device also supports real-time monitoring of bacterial growth, automate the inoculation and solution dispensing processes, and include means for reducing contamination risks. Furthermore, the device incorporates data acquisition and processing means to improve accuracy, repeatability, and efficiency, thereby facilitating reliable bacterial culture development for both research and industrial applications.

OBJECTS OF THE INVENTION

[0008] The principal object of the present invention is to overcome the disadvantages of the prior art.

[0009] An object of the present invention is to develop a device that offers an autonomous solution for bacterial culturing while maintaining stable environmental conditions to improve growth consistency and reduce manual intervention.

[0010] Another object of the present invention is to develop a device that is capable of sterile handling of bacterial samples by eliminating manual contact, thereby reducing the risk of contamination during the inoculation and culturing stages.

[0011] Another object of the present invention is to develop a device that is capable of regulating and monitoring environmental factors like temperature, humidity, lighting, and oxygen to create ideal and reproducible conditions for optimal bacterial growth and activity.

[0012] Yet, another object of the present invention is to develop a device that is capable of providing autonomous image capture and data analysis for continuous monitoring of bacterial development for enabling precise tracking of growth patterns and experimental observations.

[0013] The foregoing and other objects, features, and advantages of the present invention will become readily apparent upon further review of the following detailed description of the preferred embodiment as illustrated in the accompanying drawings.

SUMMARY OF THE INVENTION

[0014] The present invention relates to a bacteria culturing device that is capable of automating entire bacterial cultivation process by maintaining controlled environmental conditions, ensuring sterile handling, enabling precise nutrient and solution delivery, and facilitating continuous monitoring and analysis to promote consistent bacterial growth and reduce manual intervention and contamination risk.

[0015] According to an embodiment of the present invention, a bacteria culturing device is comprising, a housing installed with a motorized door to allow access for inserting and removing Petri dishes, a platform is arranged within the housing to hold one or more Petri dishes securely, a vertical extendable bar attached to an inner ceiling of the housing, the bar comprising a suction unit mounted on a motorized ball-and-socket joint, the suction unit is configured to lift lids of the Petri dishes and the joint allows multi-angle movement for lid removal, a chamber integrated within the housing and installed with a beaker for containing bacterial growth solution, the beaker incorporates a magnetic stirring unit configured to mix the solution thoroughly to maintain uniform composition, a conduit fluidly connected between the beaker and the Petri dishes, a pump is arranged on the conduit for regulating the flow of the solution from the beaker to the Petri dishes, the pump being actuated by an inbuilt microcontroller to dispense the solution in controlled and measured quantities, thereby maintaining consistency in the bacterial growth environment, a nutrient compartment subdivided into multiple sections for storing various nutrient solutions, each section being connected to the Petri dishes via tubes equipped with nozzles for precise solution delivery, each nozzle is coupled to a swivel joint for adjusting direction of solution flow, a plurality of RGB (Red Green Blue) LEDs (Light Emitting Diode) arranged inside the housing, the LEDs emitting adjustable wavelengths including red, blue, and UV (Ultraviolet) light, wherein the LEDs flicker at irregular intervals to mimic natural environmental changes for improved bacterial development, a bacterial chamber provided within the housing configured to store bacteria, the chamber being associated with a motorized injection unit and a robotic arm for automatic, contamination-free introduction of bacteria onto the Petri dishes.

[0016] According to another embodiment of the present invention, the present device is further comprising, a fluorescent light source arranged within the housing for enhanced visibility, and a camera is configured within the housing to capture high-resolution images of bacterial growth and solution content, the camera being linked to a holographic projection unit for displaying three-dimensional images for analysis, an ultrasonic generator integrated within the housing for producing low-frequency ultrasound waves at periodic intervals to increase bacterial permeability and stimulate gene transfer, an electromagnetic coil is embedded within the housing to expose bacteria to controlled low-intensity electromagnetic fields, thereby enhancing bacterial growth and stress resilience, the platform comprises of multiple suction cups to grip the Petri dishes firmly, and a motorized slider positioned between a base portion of the housing and the platform to facilitate controlled movement of the Petri dishes toward or away from the door, a Peltier unit is embedded within the housing for regulating internal temperature between 30–37degree Celsius, the Peltier unit simulates environmental conditions to promote bacterial growth, a humidifier coupled with a humidity sensor is integrated within the housing for maintaining optimal moisture levels, an oxygen pump is provided within the housing to regulate oxygen levels inside the housing, and an oxygen sensor monitors oxygen concentration and actuates the pump to adjust airflow for optimal bacterial growth conditions, an UV (Ultraviolet) light is positioned inside the housing for sterilization purposes, the UV light being periodically activated to disinfect internal surfaces and prevent contamination.

[0017] While the invention has been described and shown with particular reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

[0018] These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, appended claims, and accompanying drawings where:
Figure 1 illustrates an isometric view of a bacteria culturing device.

DETAILED DESCRIPTION OF THE INVENTION

[0019] The following description includes the preferred best mode of one embodiment of the present invention. It will be clear from this description of the invention that the invention is not limited to these illustrated embodiments but that the invention also includes a variety of modifications and embodiments thereto. Therefore, the present description should be seen as illustrative and not limiting. While the invention is susceptible to various modifications and alternative constructions, it should be understood, that there is no intention to limit the invention to the specific form disclosed, but, on the contrary, the invention is to cover all modifications, alternative constructions, and equivalents falling within the spirit and scope of the invention as defined in the claims.

[0020] In any embodiment described herein, the open-ended terms "comprising," "comprises,” and the like (which are synonymous with "including," "having” and "characterized by") may be replaced by the respective partially closed phrases "consisting essentially of," consists essentially of," and the like or the respective closed phrases "consisting of," "consists of, the like.

[0021] As used herein, the singular forms “a,” “an,” and “the” designate both the singular and the plural, unless expressly stated to designate the singular only.

[0022] The present invention relates to a bacteria culturing device that is capable of enabling cultivation of bacteria by controlling key environmental factors such as temperature, humidity, oxygen levels, and light conditions. The present device also ensures accurate and contamination-free dispensing of bacterial samples, nutrients, and growth media, with integrated real-time monitoring and analysis capabilities.

[0023] Referring to Figure 1, an isometric view of a bacteria culturing device is illustrated, comprising, a housing 101 is developed to be positioned on a flat surface, a platform 102 is arranged within the housing 101, a vertical extendable bar 103 attached to an inner ceiling of the housing 101, a suction unit 104 mounted on a motorized ball-and-socket joint 105 configured with the bar 103, a chamber 106 integrated within the housing 101 and installed with a beaker 107, the beaker 107 incorporates a magnetic stirring unit 108, a conduit 109 fluidly connected between the beaker 107 and the Petri dishes, a pump 110 is arranged on the conduit 109, a nutrient compartment 111 subdivided into multiple sections installed within the housing 101, a tubes 112 is connected to each section of the compartment 111 and equipped with nozzles 113, each nozzle is coupled to a swivel joint 114, a plurality of RGB (Red Green Blue) LEDs 115 (Light Emitting Diode) arranged inside the housing 101, a bacterial chamber 116 provided within the housing 101, the chamber 116 being associated with a motorized injection unit 117 and a robotic arm 118, a fluorescent light source 119 arranged within the housing 101, a camera 120 is configured within the housing 101, a holographic projection unit 121 installed on the housing 101, an ultrasonic generator 122 integrated within the housing 101, an electromagnetic coil 123 is embedded within the housing 101 on the platform 102, a Peltier unit 124 is embedded within the housing 101, a humidifier 125 is integrated within the housing 101, an oxygen pump 126 is provided within the housing 101, an UV (Ultraviolet) light 127 is positioned inside the housing 101, the platform 102 comprises of multiple suction cups 128, and a motorized slider 129 positioned between a base portion of the housing 101 and a motorized door 130 is installed with the housing 101.

[0024] The device disclosed herein includes a housing 101 is developed to be positioned on a flat surface. The housing 101 herein includes all necessary component of the device for assisting in bacteria culturing.

[0025] In an embodiment of the present invention, the housing 101 is cuboidal in shape, thus ensuring durability and stability.

[0026] A push button is installed on the top of the housing 101 for activating and deactivating the device. The push button is accessed by the user for activating the device. When the user presses the push button, the electrical circuit is completed, which in response turns the device on. The push button is integrated with an actuator and a spring, which are automatically activated when pressed. They work together to move the internal contact, completing the circuit and allowing electrical current to flow, thereby activating the device. On releasing the push button, the spring resets the button and returns to the open position.

[0027] When the push button is pressed, the button sends a signal (usually a change in voltage or current) to an inbuilt microcontroller associated with the device to either power up or shut down the microcontroller. Conversely, releasing the button allows the spring to return to its original position, breaking the circuit and sending the signal to deactivate the device. The microcontroller is pre-fed to detect this signal and respond accordingly. The microcontroller used herein is pre-fed using artificial intelligence and machine learning protocols to coordinate the working of the device.

[0028] A motorized door 130 is installed in front portion of the housing 101 that allows the user to access the housing 101 for inserting and removing Petri dishes from the housing 101. The motorized door 130 employed is preferably a door of sliding type which comprises of a pair of track, one attached at upper side of gate and another one is attached lower side of the gate and in between both track a door panel is fixed. There are multiple rolling members are embedded in between the track and edge of door panel which rotates and enables the translation of the door panel to open the door 130 for allowing the user inserting and removing the Petri dishes from the housing 101.

[0029] A platform 102 is installed within the housing 101 that is capable of holding one or more Petri dishes securely and equipped with multiple suction cups 128 to grip the petri dishes firmly. The multiple suction cups 128 used herein are made up of silicone rubber that easily eliminates pressure inside the suction cup and creating a vacuum between the cup and the surface of the petri dishes which creates an air-tight seal, resisting any slipping of the dishes in order to adhere the dishes on the platform 102. The suction cups 128 are engineered for easy release and reattachment without losing their suction power, allowing for convenient removing of the dishes when necessary.

[0030] A motorized slider 129 is positioned in between a base portion of the housing 101 and the platform 102 to facilitate controlled movement of the Petri dish toward or away from the door 130. The slider 129 consists of a sliding rails fabricated with grooves in which the wheel of a slider 129 is positioned that is further connected with a bi-directional motor via a shaft. The microcontroller actuates the bi-directional motor to rotate in a clockwise and anti-clockwise direction that aids in the rotation of the shaft, wherein the shaft converts the electrical energy into rotational energy for allowing movement of the wheel to translate over the sliding rail by a firm grip on the grooves. The movement of the slider 129 results in the translation of the platform 102 within the housing 101 towards or away from the door 130.

[0031] A vertical extendable bar 103 is mounted to the inner ceiling of the housing 101, and includes a suction unit 104 attached to a motorized ball-and-socket joint 105. The suction unit 104 is designed to lift the lids of Petri dish, while the joint 105 enables multi-angle movement to facilitate smooth and precise lid removal. The extension/retraction of the extendable bar 103 is powered pneumatically by the microcontroller by employing a pneumatic unit associated with the bar 103, including an air compressor, air cylinders, air valves and piston which works in collaboration to aid in extension and retraction of the bar 103. The pneumatic unit is operated by the microcontroller, such that the microcontroller actuates valve to allow passage of compressed air from the compressor within the cylinder, the compressed air further develops pressure against the piston and results in pushing and extending the piston. The piston is connected with the bar 103 and due to applied pressure the bar 103 extends and similarly, the microcontroller retracts the bar 103 by closing the valve resulting in retraction of the piston. Thus, the microcontroller regulates the extension/retraction of the bar 103 in order to position the suction unit 104 over the lid of the Petri dish for lid removal.

[0032] The motorized ball and socket joint 105 includes a motor powered by the microcontroller generating electrical current, a ball shaped element and a socket. The ball moves freely within the socket. The motor rotates the ball in various directions that is controlled by the microcontroller that further commands the motor to position the ball precisely. The microcontroller further actuates the motor to generate electrical current to rotate in the joint 105 for providing movement to the suction unit 104 for aligning the with the lid of the dish.

[0033] The suction unit 104 includes a suction cup that makes contact with the lid of the dish, a pump that operates by creating a vacuum to adhere the lid of the dish. When the pump is activated, it draws the air in through an intake. Inside the pump, a rotating impeller moves to reduce the pressure within the pump chamber. This reduction in pressure creates a vacuum effect, which generates suction and secure the lid of the dish. Once the lid is successfully secured, the microcontroller retracts the bar 103 to lift the lid in order to open the dish.

[0034] A chamber 106 integrated within the housing 101 is equipped with a beaker 107 containing a bacterial growth solution. The beaker 107 includes a magnetic stirring unit 108 designed to mix the solution thoroughly, ensuring a uniform composition. The magnetic stirring unit 108 comprises a magnetic bead controlled by a rotating external magnetic field generator integrated into the bottom of the beaker 107. The generator typically includes an electric motor or electromagnetic coil that creates a rotating magnetic field. The magnetic bead, usually coated with an inert, non-reactive material, responds to this field by rotating within the solution. This rotation creates a vortex, promoting continuous stirring of the bacterial solution. The non-contact agitation ensures uniform distribution of solutes, maintains consistent solution composition, and minimizes the risk of contamination. The magnetic stirring unit 108 thus enables reliable and homogenous mixing over extended durations, essential for stable bacterial growth conditions.

[0035] A conduit 109 is fluidly connected between the beaker 107 and the Petri dishes to facilitate the transfer of the solution. A pump 110 is installed along the v to regulate the flow of the solution from the beaker 107 to the Petri dishes. The pump 110 dispenses the solution in controlled and measured quantities, ensuring consistency in the bacterial growth environment. The pump 110 operates by generating controlled fluid movement through pressure, drawing the solution from the beaker 107 using suction produced by a motorized arrangement, such as a peristaltic or diaphragm pump. The solution is then directed through the conduit 109 and accurately dispensed into the Petri dishes, with the flow rate and volume precisely regulated to maintain uniform distribution.

[0036] A nutrient compartment 111 is subdivided into multiple sections, each designed to store a different nutrient solution. Each section is connected to the Petri dishes through individual tubes 112 fitted with nozzles 113 to enable precise delivery of the solutions. The nozzles 113 work by channeling the nutrient solution from the nutrient compartment 111 through a narrow outlet, controlling the flow rate and direction of the liquid as it is dispensed into the Petri dishes. The pressure generated by a connected pump or gravity forces the solution through the tube and out of the nozzles 113 in a controlled stream or spray, depending on the nozzle design. The nozzles 113 are attached to a swivel joint 114, which allows it to rotate or tilt in multiple directions.

[0037] The swivel joint 114 works by allowing rotational and angular movement between connected parts, in this case, between the nozzle and the tubing. The swivel joint 114 typically consists of a ball-and-socket or rotating connector that enables the nozzle to pivot or rotate freely in multiple directions. When the nozzle is mounted on the swivel joint 114, it is adjusted to change the angle or direction of fluid flow during dispensing. This flexibility ensures that the nutrient solution is accurately directed into the desired location within the Petri dishes, improving precision and reducing the risk of spills or misalignment.

[0038] The swivel joint 114 works by allowing the nozzle to rotate and tilt in multiple directions, enabling precise adjustment of the fluid flow during dispensing. The swivel joint 114 typically consists of a ball-and-socket or rotating connector that forms a flexible link between the nozzle and the fluid delivery tubing. The swivel joint 114 is integrated with a servo motor or stepper motor that controls the joint’s movement that is actuated by the microcontroller. This motor allows the nozzle to adjust its angle and direction, ensuring accurate and targeted dispensing of the nutrient solutions into the Petri dishes.

[0039] A plurality of RGB (Red, Green, Blue) LEDs 115 (Light Emitting Diodes) is installed inside the housing 101. These LEDs 115 are capable of emitting a wide range of wavelengths, including ultraviolet (UV) light, by adjusting the intensity of red, green, and blue components to create different colors and spectrums. Each LED is made from semiconductor materials and contains a p-n junction, where the p-type region is positively charged and the n-type region is negatively charged. When voltage is applied, electrons from the n-region move toward the p-region and recombine with holes. This recombination releases energy in the form of photons, producing visible light. The RGB LEDs 115 are controlled by the microcontroller to flicker at irregular intervals, causing changes in light intensity and color in an unpredictable pattern rather than in fixed cycles. This flickering simulates natural environmental variations, such as sunlight filtered through moving clouds or changing daylight conditions, similar to what bacteria experience in natural habitats. By recreating these dynamic lighting conditions, the device promotes a more natural and responsive bacterial growth environment, potentially enhancing bacterial development, adaptation, and behavioral responses that are not typically observed under constant artificial lighting.

[0040] A bacterial chamber 116 is housed within the device to store bacteria. In proximity, a motorized injection unit 117 mounted on a robotic arm 118 is installed inside the housing 101 to enable automatic, contamination-free dispensing of bacteria onto the Petri dishes. The motorized injection unit 117 works by precisely controlling the movement and dispensing of bacteria onto Petri dishes. The robotic arm 118 positions the injection unit 117 accurately over each dish. The injection unit 117 uses a motor-driven arrangement include but not limited to, such as a syringe pump or microfluidic injector, to release controlled, small volumes of bacterial solution. This injection unit 117 ensure consistent and sterile dispensing by minimizing exposure to the environment, reducing contamination risks, and improving repeatability in bacterial sample application across multiple Petri dishes.

[0041] The robotic arm 118 comprises a robotic link and a clamp attached to the link. The robotic link consists of several segments connected by joints, also referred to as axes. Each joint contains a stepper motor that rotates to enable precise movement of the arm segments. Upon actuation by the microcontroller, these motors drive the coordinated motion of the robotic link, allowing the clamp to be accurately positioned over the target location. The clamp then holds and operates the motorized injection unit 117 to dispense bacteria onto the Petri dishes automatically and contamination-free, ensuring precise and consistent application.

[0042] A fluorescent light source 119 arranged within the housing 101 for enhanced visibility. The fluorescent light source 119 works by passing an electric current through a gas-filled tube, usually containing mercury vapor. Inside the tube, electrodes at both ends emit electrons when energized, which collide with the gas atoms, producing ultraviolet (UV) light. This UV light then strikes a phosphor coating on the inside surface of the tube, causing the phosphor to fluoresce and emit visible light. The main operational components include the glass tube, mercury vapor, electrodes, phosphor coating, and an electronic ballast or starter that regulates the current flow. The fluorescent light efficiently converts electrical energy into bright, even illumination, enhancing visibility by providing consistent and widespread light output suitable for various environments.

[0043] A camera 120 is mounted within the housing 101 to capture high-resolution images of bacterial growth and solution content. The camera 120 comprises an image capturing module, including a set of lenses that capture multiple images of the bacterial growth and solution content from various angles. The captured images are stored within the memory of the imaging unit as optical data. The camera 120 also includes a processor embedded with artificial intelligence protocols. This processor performs essential image processing steps such as noise reduction to enhance clarity, feature extraction to identify relevant characteristics like shape, color, and size, and segmentation to isolate the bacterial colonies or solution features from the background. The extracted and processed data is then converted into digital pulses and bits, which are transmitted to the microcontroller. The microcontroller further processes this data to detect and analyze bacterial growth patterns and solution conditions accurately.

[0044] The camera 120 is connected to a holographic projection unit 121 mounted within the housing 101, which displays three-dimensional images for detailed analysis. The holographic projection unit 121 functions by generating and projecting holograms—three-dimensional images formed through the interference of light waves. Initially, laser light from the unit is split into two beams: an object beam, which interacts with the subject (such as bacterial growth or sample features) and is modified based on the subject’s shape and characteristics; and a reference beam, which remains unaltered. These two beams intersect, producing an interference pattern that is recorded on a photosensitive surface like a holographic plate. This pattern encodes the phase and amplitude information of the light waves, preserving the subject’s three-dimensional details. During projection, a laser beam illuminates the recorded interference pattern, diffracting the light to reconstruct the original wave fronts of both the object and reference beams. This reconstruction produces a floating, three-dimensional image that enables immersive and precise analysis.

[0045] An ultrasonic generator 122 is installed within the housing 101 for producing low-frequency ultrasound waves at periodic intervals to increase bacterial permeability and stimulate gene transfer. The ultrasonic generator 122 works by producing low-frequency ultrasound waves at periodic intervals to enhance bacterial permeability and stimulate gene transfer. The generator 122 typically includes an oscillator circuit and an ultrasonic transducer. The microcontroller controls the oscillator to generate high-frequency electrical signals. These signals drive the piezoelectric ultrasonic transducer, which converts electrical energy into mechanical vibrations, producing ultrasound waves. When directed into the bacterial solution, these waves create microscopic cavitation bubbles that temporarily disrupt cell membranes, increasing permeability and facilitating genetic material transfer. This non-invasive method improves bacterial transformation efficiency while preserving cell viability.

[0046] An electromagnetic coil 123 is installed within the housing 101 to expose bacteria to controlled low-intensity electromagnetic fields, thereby enhancing bacterial growth and stress resilience. The electromagnetic coil 123 works by generating controlled low-intensity electromagnetic fields when an electric current passes through it. The coil 123, typically made of wound conductive wire, is powered by the microcontroller. By adjusting the current flow, the microcontroller regulates the strength, frequency, and duration of the electromagnetic fields produced. When bacteria are exposed to these fields, the electromagnetic energy interacts with cellular processes, promoting enhanced growth and increasing stress resilience.

[0047] A Peltier unit 124 is installed within the housing 101 for regulating internal temperature between 30–37degree Celsius. The Peltier unit 124 simulates environmental conditions to promote bacterial growth. The Peltier unit 124 consists of two semiconductor plates, known as Peltier plates, connected in series and sandwiched between two ceramic plates. When an electric current is applied to the Peltier unit 124, one side of the unit absorbs heat from its surroundings, while the other side releases heat, thereby creating a temperature difference across the unit. In this device, the Peltier unit 124 is controlled by the microcontroller to regulate the internal temperature of the housing within a range of 30–37°C. By adjusting the current direction and intensity, the microcontroller either heat or cool the housing 101 as needed. This temperature control simulates optimal environmental conditions, promoting efficient bacterial growth, metabolic activity, and stability of the culture.

[0048] A humidifier 125 coupled with a humidity sensor is arranged within the housing 101 for maintaining optimal moisture levels. This humidity sensor is preferably a capacitive type, which operates based on variations in the dielectric properties of a material that responds to humidity changes. The sensor is composed of a humidity-sensitive layer placed between two electrodes. This layer absorbs or adsorbs water molecules from the air, and as humidity increases, its dielectric constant changes, thereby altering the capacitance between the electrodes. Higher humidity increases capacitance, while lower humidity decreases capacitance.

[0049] The sensor is linked to a sensing circuit that monitors these changes. The circuit includes an oscillator that generates an alternating current (AC) signal, with the sensor acting as part of the circuit’s capacitive load. Fluctuations in capacitance affect the signal, which is then converted into a readable output—usually a voltage or frequency. This output, which reflects the ambient humidity, is transmitted to the microcontroller. The microcontroller processes this input to determine the current humidity level.

[0050] The humidifier 125 works by adding moisture to the surrounding air to maintain a stable and optimal humidity level within the environment. The humidifier 125 typically includes a water reservoir, a mist or vapor generator (such as an ultrasonic transducer or heating element), and an outlet or fan for dispersing the moisture. In an ultrasonic humidifier 125, for example, a vibrating diaphragm produces high-frequency sound waves that break water into fine mist particles, which are then released into the air. In other types, like evaporative or warm mist humidifiers 125, moisture is either evaporated through a wick and fan or produced by boiling water. The microcontroller receives real-time humidity data from the humidity sensor and activates the humidifier 125 when moisture levels fall below a desired threshold. This ensures a consistent humidity range, which is crucial for processes like bacterial growth where controlled environmental conditions are necessary for reliable results.

[0051] An oxygen pump 126 is installed within the housing 101 to maintain appropriate oxygen levels, while an oxygen sensor continuously monitors the concentration of oxygen. Based on the detected levels, the microcontroller signals the pump to regulate airflow, ensuring optimal conditions for bacterial growth. The oxygen sensor works by measuring the concentration of oxygen in the surrounding environment and converting it into an electrical signal. The sensor typically consists of a sensing electrode, a counter electrode, and an electrolyte. When oxygen molecules enter the sensor through a permeable membrane, they reach the sensing electrode where a redox reaction occurs. This reaction generates an electrical current proportional to the amount of oxygen present. The more oxygen that reacts, the higher the current produced. This current is then measured and interpreted by a sensing circuit, which sends a corresponding signal to the microcontroller. The microcontroller processes this data to determine the exact oxygen concentration inside the housing 101 and, based on the detected level, actuates the oxygen pump 126 to regulate airflow, ensuring optimal conditions for bacterial growth.

[0052] The oxygen pump 126 regulates airflow by controlling the movement of oxygen-rich air into the housing 101 to maintain optimal levels for bacterial growth. The oxygen pump 126 typically consists of a motor-driven arrangement, include but not limited to, such as a diaphragm or peristaltic pump, which creates a controlled airflow by drawing in ambient air and pushing it into the housing 101. The pump’s operation is guided by signals from the microcontroller, which receives real-time oxygen concentration data from the electrochemical oxygen sensor. When oxygen levels drop below the desired threshold, the microcontroller activates the pump to increase airflow, supplying more oxygen. Conversely, if oxygen levels are sufficient or too high, the pump reduces or stops airflow. This precise regulation ensures a stable and balanced oxygen environment that supports healthy bacterial development.

[0053] An ultraviolet (UV) light 127 is installed inside the housing 101 for sterilization purposes. The UV light 127 is periodically activated to disinfect internal surfaces, helping to prevent contamination. The ultraviolet (UV) light 127 sterilizes by emitting high-energy UV-C rays, typically within the wavelength range of 200 to 280 nanometers. These rays penetrate the cell walls of microorganisms such as bacteria, viruses, and fungi. The UV-C light 127 damages the DNA and RNA inside these cells by causing the formation of thymine dimers, which disrupt the genetic material and prevent the microorganisms from replicating or functioning properly. This leads to their inactivation and effectively sterilizes the surface.

[0054] The UV light 127 source generally consists of a UV lamp or LED that emits the required wavelength. When powered on, the lamp produces UV-C radiation that is directed toward the internal surfaces of the housing 101. The light 127 is periodically activated according to preset intervals or sensor inputs to ensure regular disinfection without constant exposure. This non-chemical sterilization method efficiently reduces contamination risks, maintaining a sterile environment optimal for bacterial growth and experimentation.

[0055] The present invention work best in the following manner, where the housing 101 integrated with the motorized door 130 for controlled access to the interior platform 102, which securely holds multiple Petri dishes using suction cups 128. The platform 102 is movably mounted on the motorized slider 129 to facilitate precise positioning. The vertical extendable bar 103 mounted on the inner ceiling is equipped with the suction unit 104 affixed to the motorized ball-and-socket joint 105 for lifting Petri dish lids with multi-angle flexibility. The bacterial growth solution is stored in the beaker 107 located within the integrated chamber 106, where the magnetic stirring unit 108, including the magnetic bead and bottom-mounted rotating external magnetic field generator, ensures homogenous mixing. The solution flows through the conduit 109 controlled by the pump 110, which is actuated by the microcontroller for precise dispensing. The nutrient compartment 111, divided into multiple sections, connects to the Petri dishes via tubes 112 equipped with nozzles 113 mounted on the swivel joints 114 for directional fluid delivery. The RGB (Red, Green, Blue) LEDs 115 emitting red, blue, and UV light operate at irregular flicker intervals to simulate natural lighting. The bacterial chamber 116 works in coordination with the robotic arm 118 and motorized injection unit 117 for contamination-free bacterial seeding. The fluorescent light source 119 enhances visibility, while the camera 120 captures high-resolution images, linked to the holographic projection unit 121 for 3D display. The ultrasonic generator 122 emits low-frequency waves for gene transfer enhancement, and the electromagnetic coil 123 induces low-intensity electromagnetic fields to stimulate bacterial growth. The microcontroller dynamically regulates the Peltier unit 124, humidifier 125, and oxygen pump 126 based on sensor feedback to maintain optimal internal conditions.

[0056] Although the field of the invention has been described herein with limited reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the invention, will become apparent to individuals skilled in the art upon reference to the description of the invention. , Claims:1) A bacteria culturing device, comprising:

i) a housing 101 installed with a motorized door 130 to allow access for inserting and removing Petri dishes;
ii) a vertical extendable bar 103 attached to an inner ceiling of the housing 101, the bar 103 integrated with a suction unit 104 via on a motorized ball-and-socket joint 105, configured to lift lids of the Petri dishes;
iii) a chamber 106 integrated within the housing 101 and installed with a beaker 107 for containing bacterial growth solution, the beaker 107 incorporating a magnetic stirring unit 108 to mix the solution thoroughly to maintain uniform composition;
iv) a pump 110 integrated with a conduit 109 fluidly connected between the beaker 107 and the Petri dishes for regulating the flow of the solution from the beaker 107 to the Petri dishes;
v) a nutrient compartment 111 subdivided into multiple sections for storing various nutrient solutions, each section being connected to the Petri dishes via tubes 112 equipped with nozzles 113 coupled with swivel joints 114 for precise solution delivery;
vi) a plurality of RGB (Red Green Blue) LEDs 115 (Light Emitting Diode) arranged inside the housing 101 to flicker at irregular intervals to mimic natural environmental changes for enhanced bacterial development;
vii) a bacterial chamber 116 provided within the housing 101 configured to store bacteria, the chamber 116 being associated with a motorized injection unit 117 and a robotic arm 118 for introduction of bacteria onto the Petri dishes;
viii) a fluorescent light source 119 arranged within the housing 101 for enhanced visibility, and a camera 120 is configured within the housing 101 to capture high-resolution images of bacterial growth and solution content, the camera 120 being linked to a holographic projection unit 121 for displaying three-dimensional images for analysis; and
ix) an ultrasonic generator 122 integrated within the housing 101 to increase bacterial permeability and stimulate gene transfer, and an electromagnetic coil 123 is embedded within the housing 101 to enhance bacterial growth and stress resilience.

2) The device as claimed in claim 1, wherein the platform 102 comprises of multiple suction cups 128 to grip the Petri dishes firmly, and a motorized slider 129 positioned between a base portion of the housing 101 and the platform 102 to facilitate controlled movement of the Petri dishes toward or away from the door 130.

3) The device as claimed in claim 1, wherein a Peltier unit 124 is embedded within the housing 101 for regulating internal temperature between 30–37degree Celsius, the Peltier unit 124 simulates environmental conditions to promote bacterial growth.

4) The device as claimed in claim 1, wherein a humidifier 125 coupled with a humidity sensor is integrated within the housing 101 for maintaining optimal moisture levels.

5) The device as claimed in claim 1, wherein an oxygen pump 126 is provided within the housing 101 to regulate oxygen levels inside the housing 101, and an oxygen sensor monitors oxygen concentration and actuates the pump to adjust airflow for optimal bacterial growth conditions.

6) The device as claimed in claim 1, wherein an UV (Ultraviolet) light 127 is positioned inside the housing 101 for sterilization purposes, the UV light 127 being periodically activated to disinfect internal surfaces and prevent contamination.

7) The device as claimed in claim 1, wherein the microcontroller is configured to receive environmental readings from the humidity sensor, temperature control unit, and oxygen sensor and dynamically adjusts the humidifier 125, Peltier unit 124, and oxygen regulation pump to sustain target conditions for bacterial cultivation.

8) The device as claimed in claim 1, wherein the robotic arm 118 includes multiple motorized joints and an end-effector for adjusting injection unit 117 placement, enabling precision-controlled seeding of the Petri dishes.

9) The device as claimed in claim 1, wherein the magnetic stirring unit 108 comprises a magnetic bead controlled by a rotating external magnetic field generator integrated into the bottom of the beaker 107 for ensuring homogenous mixing of bacterial solutions.

10) The device as claimed in claim 1, wherein the camera 120 is configured to track bacterial colony morphology and growth rate over time, and the growth data is displayed via the holographic projection unit 121.