Description:FIELD OF INVENTION
The present invention relates to a dietary supplement comprises immuxanth powder, formulated with antioxidant-rich natural ingredients to enhance nutritional benefits and reduce oxidative stress. It aims to support overall health, improve immune function, and provide effective antioxidant protection.
BACKGROUND OF THE INVENTION
Oxidative stress, resulting from an imbalance between free radicals and the body's antioxidant defenses, is a significant contributor to various chronic conditions, including cardiovascular disorders, diabetes, neurodegenerative diseases, and age-related ailments. Free radicals and reactive oxygen species (ROS) cause cellular damage through oxidative mechanisms, leading to inflammation, tissue degeneration, and disease progression. While antioxidants play a critical role in neutralizing these harmful molecules, many conventional dietary supplements fail to offer comprehensive antioxidant protection or are limited by inadequate bioavailability and stability. This underscores the need for a potent, natural formulation that effectively mitigates oxidative stress and supports overall health.
Despite the availability of numerous dietary supplements claiming antioxidant benefits, most are formulated with isolated compounds or synthetic antioxidants, lacking the synergistic efficacy required for optimal health benefits. Additionally, the rapid degradation of certain natural antioxidants during processing and storage compromises their potency. Moreover, these supplements often overlook the importance of immune support, which is intricately linked to oxidative stress management. This limitation necessitates the development of a novel dietary supplement that integrates a scientifically validated combination of antioxidant-rich natural ingredients with immune-boosting properties for enhanced efficacy.
The present invention addresses these challenges by providing Immuxanth powder, a dietary supplement formulated with a blend of berries, including blackberries, blackcurrants, blueberries, cranberries, raspberries, and strawberries. These berries are known for their rich content of bioactive compounds such as phenolic compounds, flavonoids, tannins, and phenolic acids, which exhibit potent antioxidant, anti-inflammatory, and immune-boosting properties. This formulation offers enhanced nutritional benefits and improved resistance against oxidative stress. The carefully selected natural ingredients ensure high bioavailability, stability, and synergistic action, effectively overcoming the drawbacks of conventional supplements.
The following Prior art being Reported was:
In the field of nutraceuticals, antioxidant powders and fruit extract formulations have been extensively pursued. US 2017/0202260 A1 discloses a nutrient delivery system comprising a pod and a nutritional powder with lipophilic antioxidants, offering improved shelf life (at least 12 months) and applicable notably to synthetic infant formulas. KR 20190064697 A describes a method to produce berry powder by spray drying berry concentrates with dextrin and porous dextrin, emphasizing specific volume and surface area characteristics. US 2008/0260821 A1 relates to berry extract preparations with stable anthocyanin content via solvent extraction at low pH and possible inclusion of cryoprotectants to enhance antioxidant and anti inflammatory activity. WO 2010/137939 A1 proposes a method for producing a nutraceutical composition combining oil soluble antioxidants from edible oil and water soluble antioxidants from plant extract, emulsified, concentrated, and dried into powder form.
Despite these disclosures, there remains a need for a nutraceutical powder incorporating multiple fruit extracts standardized for diverse bioactives—including flavonoids, xanthones, carotenoids, vitamins, minerals, amino acids, fiber—with validated antioxidant efficacy, without added sugar or fat, and processed to retain stability and potency.
OBJECTS OF THE INVENTION
Some of the objects of the present disclosure, which at least one embodiment herein satisfies, are as follows.
It is an object of the present disclosure to ameliorate one or more problems of the prior art or to at least provide a useful alternative
An object of the present disclosure is to develop a nutraceutical powder using a blend of antioxidant-rich berries and fruits.
Another object of the present disclosure is to extract and preserve bioactive compounds using optimized solvent extraction techniques.
Still another object of the present disclosure is
Another object of the present disclosure is to ensure nutrient stability through controlled drying methods such as freeze drying.
Still another object of the present disclosure is to perform comprehensive nutritional profiling using standardized AOAC methods.
Still another object of the present disclosure is to identify and quantify key phytochemicals via GC-MS analysis.
Yet another object of the present disclosure is to evaluate antioxidant activity using DPPH, ABTS, FRAP, and ORAC assays.
Yet another object of the present disclosure is to assess molecular interactions of bioactives with oxidative stress targets through in silico docking.
Yet another object of the present disclosure is to formulate a stable, low-calorie, sugar-free powder suitable for health-conscious consumers.
Other objects and advantages of the present disclosure will be more apparent from the following description, which is not intended to limit the scope of the present disclosure.

SUMMARY OF THE INVENTION
The following presents a simplified summary of the invention in order to provide a basic understanding of some aspects of the invention. This summary is not an extensive overview of the present invention. It is not intended to identify the key/critical elements of the invention or to delineate the scope of the invention. Its sole purpose is to present some concept of the invention in a simplified form as a prelude to a more detailed description of the invention presented later.

The present invention is generally relates to an immuxanth powder is formulated using extracts from multiple berries and fruits like gooseberry, acai berry, cranberry, and goji berry. These ingredients are selected for their high antioxidant and nutritional content.
An embodiment of the present invention the bioactive compounds are extracted using aqueous or hydroalcoholic solvents, ensuring maximum retention of polyphenols, flavonoids, and xanthones. Temperature and pressure are carefully controlled to maintain extract potency.
Another embodiment of the invention is concentrated extracts are dried via freeze drying, spray drying, or vacuum oven drying at temperatures below 105 °C. This preserves heat-sensitive vitamins and maintains powder stability.
Yet another embodiment of the invention is the powder undergoes proximate analysis, vitamin and mineral quantification, amino acid profiling, and GC-MS for compound identification. AOAC methods ensure accuracy and reproducibility.
Yet another embodiment of the invention is DPPH, ABTS, FRAP, and ORAC assays confirm strong free radical scavenging potential. Results indicate the presence of synergistic antioxidant compounds.
Yet another embodiment of the invention is bioactives are docked against oxidative stress-related proteins like lipoxygenase and xanthine oxidase. Docking studies support the antioxidant potential at the molecular level.
Yet another embodiment of the invention is the composition is fat-free, sugar-free, and low-calorie, with high protein and fiber content. It supports digestion, metabolic health, and calorie-controlled diets.
Yet another embodiment of the invention is the powder is packed in moisture-proof containers under inert conditions. It is suitable for use as a standalone supplement or as an additive in functional foods.
Yet another embodiment of the invention is the nutraceutical composition in powder form, per single serving dose, comprising:
a) blackcurrant (ribes nigrum) fruit extract in an amount of 200 to 230 mg;
b) blueberry (vaccinium corymbosum) fruit extract in an amount of 210 to 230 mg;
c) raspberry (rubus idaeus) fruit extract in an amount of 180 to 200 mg;
d) strawberry (fragaria vesca) fruit extract in an amount of 200 to 230 mg;
e) mangosteen (garcinia mangostana) pericarp extract in an amount of 100 to 150 mg;
f) vitamin c (ascorbic acid) in an amount of 60 to 80 mg;
g) cranberry (vaccinium macrocarpon) fruit extract in an amount of 210 to 220 mg;
h) goji berry (lycium barbarum) fruit extract in an amount of 200 to 210 mg;
i) cherry (prunus avium) fruit extract in an amount of 140 to 150 mg;
j) bilberry (vaccinium myrtillus) fruit extract in an amount of 150 to 160 mg;
k) redberry fruit extract in an amount of 180 to 200 mg;
l) elderberry (sambucus nigra) fruit extract in an amount of 210 to 230 mg;
m) huckleberry (vaccinium spp.) fruit extract in an amount of 200 to 230 mg;
n) mulberry (morus alba) fruit extract in an amount of 220 to 230 mg;
o) stone berry fruit extract in an amount of 220 to 230 mg;
p) sea buckthorn (hippophae rhamnoides) fruit extract in an amount of 220 to 230 mg;
q) grape (vitis vinifera) fruit extract in an amount of 200 to 210 mg;
r) acai berry (euterpe oleracea) fruit extract in an amount of 180 to 200 mg;
s) sucralose in an amount of 30 to 60 mg;
t) fructooligosaccharides (fos, from chicory root) in an amount of 2 to 5 g;
u) potassium chloride in an amount of 40 to 70 mg;
v) sodium chloride in an amount of 50 to 70 mg;
w) citric acid (ins 330) in an amount of 100 to 150 mg.
Yet another embodiment of the invention is the method of preparing a nutraceutical composition in powder form, the method comprising:
a) selecting and cleaning high-quality berries and fruit sources rich in polyphenols, flavonoids, and xanthones;
b) extracting bioactive compounds from the selected materials using an aqueous or hydroalcoholic solvent at a solid-to-solvent ratio of about 1:10;
c) filtering the obtained extracts to remove solid residues;
d) concentrating the filtrates under reduced pressure at a temperature not exceeding 50 °C;
e) drying the concentrated extracts by a method selected from freeze drying, spray drying, or vacuum oven drying at a temperature not exceeding 105 °C to obtain extract powders;
f) blending the extract powders with carriers selected from maltodextrin and gum arabic;
g) adding sucralose, fructooligosaccharides (FOS), salts, and citric acid to the blend;
h) sieving the mixture to achieve a particle size corresponding to 80–100 mesh; and
i) packaging the final powder in moisture-proof containers under an inert atmosphere.
Yet another embodiment of the invention is wherein the powder exhibits a proximate composition per serving comprising 5.26 Kcal of energy, 1.90 g of protein, 1.25 g of carbohydrates, and 1.80 g of crude fiber, with no detectable fat or added sugar; wherein the composition contains essential vitamins including Vitamin A, Vitamin C, Vitamin D, Vitamin E, Vitamin B12 and Folate as determined by AOAC standardized methods; wherein the mineral profile includes sodium, potassium, calcium, magnesium, iron, copper, zinc, and selenium as analyzed using AOAC methods; wherein the total essential amino acids are present at 79.9 mg/g and non-essential amino acids at 141 mg/g including 41.9 mg/g of glutamic acid and 28.6 mg/g of histidine; wherein GC-MS analysis confirms the presence of xanthones (Emblicanin A and B), Vitamin C, and a plurality of flavonoids including Cyanidin-3-glucoside, Delphinidin derivatives, Proanthocyanidin A2, Malvidin-3-glucoside, and Zeaxanthin; wherein the composition exhibits antioxidant activity with values of 14.69 ± 0.32 mg TEAC/g dw (DPPH), 14.32 ± 0.06 mg TEAC/g dw (ABTS), 14.23 ± 0.09 mg TEAC/g dw (FRAP), and 122.69 µmol Trolox/g FW (ORAC); and wherein in silico molecular docking shows binding affinities of the said compounds with oxidative stress-related enzymes including Lipoxygenase (PDB ID: 1N8Q), NADPH oxidase (PDB ID: 2CDU), and Xanthine oxidase (PDB ID: 3NRZ) with significant negative ΔG values, indicating strong antioxidant potential.
DETAILED DESCRIPTION OF THE INVENTION
The following description is of exemplary embodiments only and is not intended to limit the scope, applicability or configuration of the invention in any way. Rather, the following description provides a convenient illustration for implementing exemplary embodiments of the invention. Various changes to the described embodiments may be made in the function and arrangement of the elements described without departing from the scope of the invention.

BIOLOGICAL SOURCE AND GEOGRAPHICAL ORIGIN
To comply with the disclosure of biological source and geographical origin requirements, the Applicant identifies that all plant materials used for the Immuxanth formulation are obtained in India, with primary procurement and processing undertaken in Lucknow, Uttar Pradesh.
Blackcurrant (Ribes nigrum) – Fruit Extract
Blueberry (Vaccinium corymbosum) – Fruit Extract
Raspberry (Rubus idaeus) – Fruit Extract
Strawberry (Fragaria vesca) – Fruit Extract
Mangosteen (Garcinia mangostana) – Pericarp Extract
Vitamin C (Ascorbic Acid) – Synthetic
Cranberry (Vaccinium macrocarpon) – Fruit Extract
Goji Berry (Lycium barbarum) – Fruit Extract
Cherry (Prunus avium) – Fruit Extract
Bilberry (Vaccinium myrtillus) – Fruit Extract
Black Currant (Ribes nigrum) – Fruit Extract
Redberry – Fruit Extract
Elderberry (Sambucus nigra) – Fruit Extract
Huckleberry (Vaccinum spp.) – Fruit Extract
Mulberry (Morus alba) – Fruit Extract
Stone Berry – Fruit Extract
Sea Buckthorn (Hippophae rhamnoides) – Fruit Extract
Grape (Vitis vinifera) – Fruit Extract
Acai Berry (Euterpe oleracea) – Fruit Extract
Sucralose – Synthetic
FOS (Fructooligosaccharides, from Chicory root) – Isolated Compound
Potassium Chloride – Synthetic
Sodium Chloride – Synthetic
Acidity Regulator (INS330 – Citric Acid) – Synthetic

The present invention relates to a novel nutraceutical formulation known as Immuxanth powder, which is derived from a synergistic blend of multiple antioxidant-rich berries and fruits including gooseberry, acai berry, cranberry, goji berry, and other xanthone-containing sources. Designed to promote general wellness and combat oxidative stress, the formulation is rich in essential vitamins, minerals, amino acids, flavonoids, xanthones, and polyphenols. The invention includes a standardized method of preparation involving careful selection, solvent-based extraction, concentration, and drying under controlled conditions to preserve the integrity of bioactive compounds. Advanced analytical techniques such as GC-MS and AOAC-approved methods are used for profiling the nutritional and phytochemical content. The powder demonstrates strong antioxidant activity as confirmed by in vitro assays (DPPH, ABTS, FRAP, ORAC) and in silico docking studies against oxidative stress-related enzymes. Free from added sugar and fat, and low in calories, Immuxanth powder offers a functional and stable supplement for dietary and therapeutic use.

EXAMPLE 1: Composition of the Supplement
The composition of the Immuxanth powder is developed by preparing a synergistic blend of fruit and berry extracts. The selected raw materials include blackberries, blackcurrants, blueberries, cranberries, raspberries, strawberries, elderberries, gooseberries, acai berries, cherries, bilberries, mulberries, huckleberries, redberries, stoneberries, sea buckthorn, and grape seed extract, along with mangosteen peel extract standardized to 40% alpha-mangostin. Each fruit is selected based on phytochemical content analysis. The raw materials are weighed according to predetermined proportions, washed thoroughly with potable water, and dried at controlled temperature to remove surface moisture. The dried fruits are then pulverized to coarse powder for extraction. The blending of extracts follows specific weight ratios to ensure a uniform concentration of polyphenols, flavonoids, xanthones, carotenoids, vitamins, and fibers. Natural stabilizers such as gum arabic or maltodextrin are added to prevent clumping and to enhance storage stability.
The dietary supplement composition comprises:
A composition comprising, per single serving, in an amount of: Blackcurrant (Ribes nigrum) fruit extract 200–230 mg; Blueberry (Vaccinium corymbosum) fruit extract 210–230 mg; Raspberry (Rubus idaeus) fruit extract 180–200 mg; Strawberry (Fragaria vesca) fruit extract 200–230 mg; Mangosteen (Garcinia mangostana) pericarp extract 100–150 mg; Vitamin C (ascorbic acid) 60–80 mg; Cranberry (Vaccinium macrocarpon) fruit extract 210–220 mg; Goji berry (Lycium barbarum) fruit extract 200–210 mg; Cherry (Prunus avium) fruit extract 140–150 mg; Bilberry (Vaccinium myrtillus) fruit extract 150–160 mg; Black Currant (Ribes nigrum) fruit extract 220–230 mg; Redberry fruit extract 180–200 mg; Elderberry (Sambucus nigra) fruit extract 210–230 mg; Huckleberry (Vaccinum spp.) fruit extract 200–230 mg; Mulberry (Morus alba) fruit extract 220–230 mg; Stone Berry fruit extract 220–230 mg; Sea Buckthorn (Hippophae rhamnoides) fruit extract 220–230 mg; Grape (Vitis vinifera) fruit extract 200–210 mg; Acai Berry (Euterpe oleracea) fruit extract 180–200 mg; Sucralose 30–60 mg; Fructooligosaccharides (FOS, from chicory root) 2–5 g; Potassium chloride 40–70 mg; Sodium chloride 50–70 mg; and Citric acid (INS 330) 100–150 mg.
EXAMPLE 2: Method of Preparation
The preparation of Immuxanth begins with the selection of high-quality berries and fruit sources that are naturally rich in polyphenols, flavonoids, and xanthones. The chosen raw materials are thoroughly cleaned and carefully sorted to remove impurities and non-conforming matter.
Bioactives are then extracted using aqueous or hydroalcoholic solvents, commonly 70% ethanol, maintained at a solid-to-solvent ratio of 1:10. Following extraction, the liquid extracts are filtered to remove insoluble residues. The clarified extracts are concentrated under reduced pressure at temperatures not exceeding 50 °C, a condition selected to retain heat-sensitive constituents and preserve the native antioxidant profile.
The concentrated extracts are dried by one of the following methods: freeze drying, spray drying, or vacuum oven drying, with the drying temperature controlled at or below 105 °C to protect vitamins and antioxidant compounds. The resulting extract powders are blended with suitable carriers such as maltodextrin or gum arabic, after which sucralose, fructooligosaccharides (FOS), salts, and citric acid are incorporated as required. Uniform particle size is achieved by sieving the blended powder through 80–100 mesh.
Finally, the prepared powder is packaged in moisture-proof containers under an inert atmosphere to maintain stability during storage and distribution.
EXAMPLE 4: Proximate Analysis and Nutrient Profiling
The proximate composition of Immuxanth powder was determined by using standardized methods, by the Association of Official Analytical Chemists (AOAC)
Dry matter content was measured by drying the samples at 105 °C until a constant weight was achieved using an Indian drying oven.
Crude protein (CP) was quantified using a Büchi B-324 distillation unit, where nitrogen content was multiplied by 6.25. Crude fiber (CF) content was assessed following sequential treatment with 1.25% H₂SO₄ and 1.25% NaOH using an Fiber Analyser or equivalent Indian fiber analyzer.
Total carbohydrates were calculated as Nitrogen-Free Extract (NFE) using the formula: NFE (%) = 100 - % (moisture + crude protein + crude fat + crude ash + crude fiber).
Vitamin content profiling was conducted using specific AOAC methods for accurate quantification. Vitamin C (Ascorbic Acid) was measured according to AOAC Official Method 967.24, Vitamin A (Retinol) by AOAC Method 970.64, Vitamin D (Calciferols) by AOAC Method 994.10, Vitamin E (Tocopherols) by AOAC Method 993.14, Vitamin B12 (Cyanocobalamin) by AOAC Method 944.12, and Folate (Folic Acid) by AOAC Method 2005.06. These methods ensured precise identification and quantification of essential vitamins contributing to the powder’s nutritional value.
Mineral analysis was performed to determine the content of essential minerals using AOAC standard procedures. Sodium (Na), Potassium (K), Calcium (Ca), and Magnesium (Mg) were analyzed by AOAC Method 968.08; Iron (Fe) and Copper (Cu) by AOAC Method 985.35; Zinc (Zn) by AOAC Method 990.08; and Selenium (Se) by AOAC Method 999.10. Additionally, amino acid profiling was conducted using AOAC Method 982.30.
Results show that the nutritional composition of imuuxanth powder per serving includes 5.26 Kcal of energy, 1.90g of protein, 1.25g of carbohydrates, and 1.80g of fiber, with no detectable fat or added sugar, highlighting its low-calorie, protein-rich, and fiber-dense profile ideal for health-conscious consumers.
Imuuxanth powder is characterized by a low energy content of 5.26 Kcal per serving, making it suitable for individuals aiming to manage calorie intake or incorporate it into a low-calorie diet. It provides 1.90g of protein per serving, which, while moderate, contributes positively to the overall daily protein intake when consumed alongside other dietary sources. Additionally, the formulation is devoid of fat and added sugar, presenting a favorable profile for those seeking to reduce fat and sugar consumption, whether for weight management, metabolic health, or glycemic control. The carbohydrate content is minimal at 1.25g per serving, whereas the dietary fiber content is relatively high at 1.80g per serving, which aids in digestion, promotes bowel regularity, and supports satiety.
The total essential amino acid content is 79.9 mg/g, which is significant for supporting critical physiological functions, including muscle growth, repair, enzyme function, and overall metabolic health. Non-essential amino acids are present at a total concentration of 141 mg/g, with glutamic acid constituting a 41.9 mg/g. This non-essential amino acid plays a vital role in neurotransmitter function and protein synthesis. Histidine, an essential amino acid critical for metabolic processes, is present at an amount of 28.6 mg/g.
EXAMPLE 5: GC-MS analysis
EXAMPLE 4: GC-MS Analysis
Gas Chromatography-Mass Spectrometry (GC-MS) analysis is conducted to identify bioactive compounds in Immuxanth powder. Approximately 1 g of powder is dissolved in methanol and filtered through a 0.22 µm syringe filter. The filtrate is injected into an Agilent 7890A GC system equipped with an Elite 1 capillary column (30 m length, 0.25 mm internal diameter, and 1 µm film thickness). Helium is used as the carrier gas at a constant flow rate of 1 ml/min. The injector temperature is set to 250 °C and the oven temperature program starts at 40 °C, held for 5 minutes, then increased gradually to 280 °C. Injection volume is 1 µl in split mode. The mass spectrometer is operated in electron ionization mode at 70 eV, scanning a range of 50–600 m/z. The resulting spectra are compared with the NIST Mass Spectra Database to identify compounds.
Results show that the GC-MS analysis of imuuxanth powder revealed a diverse range of bioactive compounds, including Emblicanin A and B (xanthones), Vitamin C (ascorbic acid), various flavonoids such as Cyanidin-3-glucoside, Cyanidin-3-rutinoside, Delphinidin-3-glucoside, Delphinidin-3-rutinoside, Cyanidin-3-sophoroside, Delphinidin-3-galactoside, Cyanidin-3-sambubioside, and Cyanidin-3-galactoside, along with Proanthocyanidin A2, Malvidin-3-glucoside, and Zeaxanthin. These compounds, known for their antioxidant, anti-inflammatory, and health-promoting properties, contribute to the formulation’s potential therapeutic benefits.
The presence of xanthones and flavonoids is particularly significant due to their established antioxidative, cardioprotective, and anti-inflammatory effects, while Zeaxanthin, a potent carotenoid, offers additional protection against oxidative stress and supports ocular health.
EXAMPLE 6 : Anti-Oxidant Activity
DPPH Radical Scavenging Assay:
The DPPH radical scavenging activity of Immuxanth powder was evaluated. A stock solution of stable DPPH radical was prepared by dissolving 2.4 mg of DPPH in 100 ml of methanol. A test solution containing 5 μl of Immuxanth powder was added to 3.995 ml of methanolic DPPH radical solution. The mixture was vigorously shaken and kept at room temperature in the dark for 30 minutes. The absorbance of the reaction mixture was measured at 515 nm using a spectrophotometer. A blank without Immuxanth powder was also measured. The DPPH scavenged percentage was calculated using the equation.
DPPH Scavenged (%) = ((AB - AA) / AB) × 100
Where AB is the absorbance of the blank at t=0 min, and AA is the absorbance of the antioxidant at t=30 min. A calibration curve was constructed using the % DPPH scavenged versus the concentration of the standard antioxidant (Trolox).
Results show that the DPPH assay demonstrated antioxidant activity ranging from 14.32 to 14.90 mg Trolox equivalent per gram of dry weight (mg TEAC/g dw), indicating the presence of active antioxidant compounds capable of neutralizing stable DPPH radicals.
ABTS Radical Scavenging Assay:
The ABTS radical scavenging activity of Immuxanth powder was determined using the ABTS cation decolorization assay . ABTS radical cation was generated by reacting 7 mM ABTS with 2.45 mM potassium persulfate in water. The solution was stored in the dark at room temperature for 12-16 hours before use. The ABTS cation solution was diluted with methanol to achieve an absorbance of 0.700 at 734 nm. A test solution containing 5 μl of Immuxanth powder was added to 3.995 ml of diluted ABTS cation solution, and the absorbance was measured after 30 minutes of initial mixing. A solvent blank was run in each assay. The ABTS scavenging effect was calculated as follows:
ABTS•+ Scavenging Effect (%) = ((AB - AA) / AB) × 100
Where AB is the absorbance of ABTS radical + methanol, and AA is the absorbance of ABTS radical + Immuxanth powder extract/standard (Trolox).
The ABTS assay results revealed that the antioxidant activity values between 14.01 and 14.62 mg TEAC/g dw, which were higher compared to the DPPH assay results, indicating a stronger antioxidant capacity against ABTS radicals.
Ferric Reducing Antioxidant Power (FRAP) Assay:
The FRAP reagent, containing 300 mM acetate buffer, 10 ml of TPTZ in 40 mM HCl, and 20 mM FeCl3.6H2O in the proportion of 10:1:1, was prepared and incubated at 37°C. Freshly prepared working FRAP reagent (3.995 ml) was mixed with 5 μl of appropriately diluted Immuxanth powder, and the mixture was incubated at 37°C for 30 minutes. The absorbance of the intense blue color complex formed by the reduction of Fe3+ TPTZ to Fe2+ was measured at 593 nm against a reagent blank (3.995 ml FRAP reagent + 5 μl distilled water). The FRAP values were determined by comparing the absorbance change in the test mixture with those obtained from increasing concentrations of Fe3+ and expressed as mg of Trolox equivalent per gram of Immuxanth powder.
The FRAP assay, which measures the reducing potential of antioxidants, produced values ranging from 14.12 mg to 14.5 mg TEAC/g dw.
Antioxidant and Nutritional Properties:
Parameter Value
ORAC Value 122.69 µmol Trolox/g FW
DPPH Assay 14.69±0.32
ABTS Assay 14.32±0.06
FRAP Assay 14.23±0.09
The varied results from these assays indicate the presence of multiple antioxidant compounds within the formulation, contributing to its overall antioxidant potential. This synergistic interaction among various antioxidants enhances the efficacy of immuxanth powder in neutralizing oxidative stress, and for promoting overall health and well-being through its robust antioxidant properties.
EXAMPLE 7: In Silico
Molecular docking studies were conducted to assess the antioxidant potential of various bioactive compounds derived from berries used in Immuxanth powder, including Gooseberry, Acai berry, Xanthone, Cranberry, Strawberry, Gojiberry, Cherry, Raspberry, Bilberry, Black Currant, Blueberry, Redberry, Elderberry, Huckleberry, Mulberry, Stoneberry, and Sea Buckthorn.
The 3D structures of the target proteins were obtained from the RCSB Protein Data Bank (PDB) in pdb format, while antioxidant compound structures were retrieved from the PubChem database in sdf format and converted to pdb format using Open Babel 2.4.1.
Docking simulations were performed using AUTODOCK with grid box coordinates and sizes determined. Docking scores, expressed as ∆G binding energy values (kcal/mol), were generated to evaluate binding affinity. Visualization and analysis of protein-ligand interactions were carried out using PyMOL v2 and BIOVIA Discovery Studio 2021, focusing on hydrogen bonding, hydrophobic interactions, and other critical contacts.
The antioxidant activity of the compounds was assessed against three key protein targets associated with oxidative stress: Lipoxygenase (PDB ID: 1N8Q), NADPH-oxidase (PDB ID: 2CDU), and Xanthine oxidase (PDB ID: 3NRZ). These proteins are known to oxidative stress-related disorders, making them relevant targets for antioxidant evaluation.
EXAMPLE 8: Efficacy Data
The composition’s performance is greater than the calculated additive response of its individual botanical constituents and greater than the best single component, and the synergistic effect is contingent on the defined ratios.
While considerable emphasis has been placed herein on the specific features of the preferred embodiment, it will be appreciated that many additional features can be added and that many changes can be made in the preferred embodiment without departing from the principles of the disclosure. These and other changes in the preferred embodiment of the disclosure will be apparent to those skilled in the art from the disclosure herein, whereby it is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation.
, Claims:We Claim,
1. A nutraceutical composition in powder form, per single serving dose, comprising:
a) blackcurrant (ribes nigrum) fruit extract in an amount of 200 to 230 mg;
b) blueberry (vaccinium corymbosum) fruit extract in an amount of 210 to 230 mg;
c) raspberry (rubus idaeus) fruit extract in an amount of 180 to 200 mg;
d) strawberry (fragaria vesca) fruit extract in an amount of 200 to 230 mg;
e) mangosteen (garcinia mangostana) pericarp extract in an amount of 100 to 150 mg;
f) vitamin c (ascorbic acid) in an amount of 60 to 80 mg;
g) cranberry (vaccinium macrocarpon) fruit extract in an amount of 210 to 220 mg;
h) goji berry (lycium barbarum) fruit extract in an amount of 200 to 210 mg;
i) cherry (prunus avium) fruit extract in an amount of 140 to 150 mg;
j) bilberry (vaccinium myrtillus) fruit extract in an amount of 150 to 160 mg;
k) redberry fruit extract in an amount of 180 to 200 mg;
l) elderberry (sambucus nigra) fruit extract in an amount of 210 to 230 mg;
m) huckleberry (vaccinium spp.) fruit extract in an amount of 200 to 230 mg;
n) mulberry (morus alba) fruit extract in an amount of 220 to 230 mg;
o) stone berry fruit extract in an amount of 220 to 230 mg;
p) sea buckthorn (hippophae rhamnoides) fruit extract in an amount of 220 to 230 mg;
x) grape (vitis vinifera) fruit extract in an amount of 200 to 210 mg;
y) acai berry (euterpe oleracea) fruit extract in an amount of 180 to 200 mg;
z) sucralose in an amount of 30 to 60 mg;
aa) fructooligosaccharides (fos, from chicory root) in an amount of 2 to 5 g;
bb) potassium chloride in an amount of 40 to 70 mg;
cc) sodium chloride in an amount of 50 to 70 mg;
dd) citric acid (ins 330) in an amount of 100 to 150 mg.
2. A method of preparing a nutraceutical composition in powder form, the method comprising:
a) selecting and cleaning high-quality berries and fruit sources rich in polyphenols, flavonoids, and xanthones;
b) extracting bioactive compounds from the selected materials using an aqueous or hydroalcoholic solvent at a solid-to-solvent ratio of about 1:10;
c) filtering the obtained extracts to remove solid residues;
d) concentrating the filtrates under reduced pressure at a temperature not exceeding 50 °C;
e) drying the concentrated extracts by a method selected from freeze drying, spray drying, or vacuum oven drying at a temperature not exceeding 105 °C to obtain extract powders;
f) blending the extract powders with carriers selected from maltodextrin and gum arabic;
g) adding sucralose, fructooligosaccharides (FOS), salts, and citric acid to the blend;
h) sieving the mixture to achieve a particle size corresponding to 80–100 mesh; and
i) packaging the final powder in moisture-proof containers under an inert atmosphere.
3. The composition as claimed in claim 1, wherein the powder exhibits a proximate composition per serving comprising 5.26 Kcal of energy, 1.90 g of protein, 1.25 g of carbohydrates, and 1.80 g of crude fiber, with no detectable fat or added sugar.
4. The composition as claimed in claim 1, wherein the composition contains essential vitamins including Vitamin A, Vitamin C, Vitamin D, Vitamin E, Vitamin B12 and Folate as determined by AOAC standardized methods;
5. The composition as claimed in claim 1, wherein the mineral profile includes sodium, potassium, calcium, magnesium, iron, copper, zinc, and selenium as analyzed using AOAC methods;
6. The composition as claimed in claim 1, wherein the total essential amino acids are present at 79.9 mg/g and non-essential amino acids at 141 mg/g including 41.9 mg/g of glutamic acid and 28.6 mg/g of histidine;
7. The composition as claimed in claim 1, wherein GC-MS analysis confirms the presence of xanthones (Emblicanin A and B), Vitamin C, and a plurality of flavonoids including Cyanidin-3-glucoside, Delphinidin derivatives, Proanthocyanidin A2, Malvidin-3-glucoside, and Zeaxanthin;
8. The composition as claimed in claim 1, wherein the composition exhibits antioxidant activity with values of 14.69 ± 0.32 mg TEAC/g dw (DPPH), 14.32 ± 0.06 mg TEAC/g dw (ABTS), 14.23 ± 0.09 mg TEAC/g dw (FRAP), and 122.69 µmol Trolox/g FW (ORAC)
Dated this 30 August 2025

Dr. Amrish Chandra
Agent of the applicant
IN/PA No: 2959