I/We claim:
1. A system comprising:
(a) a fatty acid decarboxylase or a fragment thereof, wherein the fatty acid
decarboxylase is selected from UndB decarboxylase or a variant thereof;
5 (b) a nicotinamide-cofactor;
(c) at least one redox couple for transferring electrons from the
nicotinamide cofactor to the fatty acid decarboxylase; and
(d) a regeneration system comprising a nicotinamide-cofactor dependent
dehydrogenase, and a substrate.
10 2. The system as claimed in claim 1, wherein the fatty acid decarboxylase is UndB
decarboxylase comprising a polypeptide having an amino acid sequence of at least 40%
sequence identity to SEQ ID NO. 1.
3. The system as claimed in any one of claims 1 or 2, wherein said system comprises a
catalase enzyme or fragment thereof, preferably Catalase HPII enzyme or a homologue
15 thereof, proximal to the fatty acid decarboxylase, preferably at a concentration ranging
from 0.1 to 20 µM.
4. The system as claimed in claim 3, wherein said catalase enzyme comprises a
polypeptide having an amino acid sequence of at least 50% sequence identity to SEQ
ID NO. 3.
20 5. The system as claimed in claim 1, wherein said variant is UndB fusion protein
comprising UndB decarboxylase or a fragment thereof, and catalase enzyme or a
fragment thereof, preferably Catalase HPII enzyme or a homologue thereof.
6. The system as claimed in claim 5, wherein said UndB fusion protein comprises a
polypeptide having an amino acid sequence of at least 60% sequence identity to SEQ
25 ID NO. 2.
7. The system as claimed in claim 1, wherein the nicotinamide cofactor is selected from
nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide
phosphate (NADPH), phenylethyl nicotinamide (P2NAH), thionicotinamide adenine
dinucleotide (thio-NADH), 3-acetylpyridine adenine dinucleotide (APADH), N30 methyl-1,4-dihydronicotinamide (MNAH) and N-benzyl-1,4-dihydronicotinamide
(BNAH), or an analogue thereof.
8. The system as claimed in claim 1, wherein concentration of the nicotinamide cofactor
ranges from 50 µM to 2000 µM, preferably 100 µM to 200 µM.
34
9. The system as claimed in claim 1, wherein the dehydrogenase enzyme is selected from
glucose dehydrogenase, glutamate dehydrogenase, mannitol dehydrogenase, phosphite
dehydrogenase, formate dehydrogenase, or combination thereof; and wherein the
substrate is selected from glucose, galactose, xylose, fructose, glutamate, mannitol,
5 phosphite, formate, or combination thereof.
10. The system as claimed in claim 1, wherein the redox couple is selected from ferredoxin
and ferredoxin reductase; putidaredoxin and putidaredoxin reductase; cytochrome b5
and cytochrome b5 reductase; or combination thereof, preferably ferredoxin and
ferredoxin reductase.