Claims:1. A biocompatible implant, comprising:
a metallic implant coated with a hybrid nanocomposite,
wherein the hybrid nanocomposite comprises nanoparticles of bone cement doped with gold (Au) nanoparticles.
wherein the nanoparticles of bone cement comprise size ranging from 100 to 500nm and the gold nanoparticles comprise size less than 50nm; and
wherein the hybrid nanocomposite comprises the nanoparticles of bone cement and the gold (Au) nanoparticles in a percentage ratio of 3:0.5.

2. A biocompatible scaffold, comprising:
nanoparticles of polymeric calcium phosphate doped with noble metal gold (Au) nanoparticles forming a hybrid nanocomposite; and
at least one nano additive, wherein the nano additive comprises collagen and chitosan,
wherein the nanoparticles of polymeric calcium phosphate comprise size ranging from 100 to 500nm,
wherein the gold nanoparticles comprise size less than 50nm.

3. A process for synthesizing a biocompatible bone cement, comprising:

generating a hybrid nanocomposite by doping nanoparticles of bone cement with gold (Au) nanoparticles using in-situ microwave technique,
wherein the nanoparticles of bone cement comprise size ranging from 100 to 500nm;
wherein the gold nanoparticles comprise size less than 50nm.
4. The process as claimed in claim 3, wherein the nanoparticles of bone cement is prepared using polymeric calcium phosphate.

5. The process as claimed in claim 3, wherein the hybrid nanocomposite comprises the nanoparticles of bone cement and the gold (Au) nanoparticles in a percentage ratio of 3: 0.5.

6. The process as claimed in claim 3, wherein the in-situ microwave technique being carried at a temperature ranging between 80 to 100°C for a duration of 10 to 30 minutes.

Dated this 20th day of February 2020

Signature

Vidya Bhaskar Singh Nandiyal
Patent Agent (IN/PA-2912)
Agent for the Applicant
, Description:FIELD OF INVENTION

[0001] Embodiment of the present disclosure relates to a tissue engineering and bone graft, more particularly it relates to a biocompatible scaffold and bone implant for tissue engineering, and process for synthesizing a biocompatible implant, bone cement composition.

BACKGROUND

[0002] In healthcare an artificial implant having huge requirement for betterment of patient’s health conditions. An implant is a manufactured to replace support a damaged biological structure, a missing biological structure, or enhance an existing biological structure. Currently, implants are manufactured using solid metal, most often titanium, stainless steel and cobalt chromium alloy. These implants are fixed in a body using a bone cement.

[0003] Polymethyl methacrylate (PMMA) is acclaimed as the bone cement and is widely used for implant fixation in various Orthopaedic and trauma surgery. The bone cement is used primarily as a filler to cement the implant to bone in hip, knee and other joint replacements. The bone cement is prepared by mixing methyl methacrylate powder with liquid methyl methacrylate monomer, which exhibits an exothermic polymerization reaction i.e. formation of polymethylmethacrylate. The polymerization reaction causes intramedullary hypertension which can cause embolization of marrow, fat and cement. The bone cement implantation syndrome causes arrhythmias, hypotension, hypoxia and has led to death.

[0004] Recent studies, using hydroxyapatite for preparing the bone cement. This improves connection to the bone as hydroxyapatite is a main inorganic constituent of bone tissue, although this compromises the mechanical strength of the cement. The bone cement prepared using hydroxyapatite being coated onto the implant in order to enhance the osseointegration and surface properties of the implant. Currently, there are wide range of bioactive materials and coating techniques for modifying implant surfaces.

[0005] However, there is need for a simple, convenient and time saving process for synthesizing a biocompatible implant.

[0006] Present disclosure provides a simple, convenient and time saving process for synthesizing a biocompatible bone cement and scaffolds based on a biocompatible bone cement composition suitable for bone graft and tissue engineering.

SUMMARY

[0007] In accordance with an embodiment of the present disclosure, a process for synthesizing a biocompatible bone cement is provided. The process includes generating a hybrid nanocomposite by doping nanoparticles of bone cement with gold (Au) nanoparticles using in-situ microwave technique.

[0008] In accordance with another embodiment of the present disclosure, a biocompatible implant is provided. The biocompatible implant includes a metallic implant coated with a hybrid nanocomposite. The hybrid nanocomposite comprises nanoparticles of bone cement doped with gold (Au) nanoparticles.

[0009] In yet accordance with another embodiment of the present disclosure, a biocompatible scaffold being provided. The biocompatible scaffold includes nanoparticles of polymeric calcium phosphate doped with noble metal gold (Au) nanoparticles forming a hybrid nanocomposite. The biocompatible scaffold also includes at least one nano additive.

[0010] To further clarify the advantages and features of the present invention, a more particular description of the invention will follow by reference to specific embodiments thereof, which are illustrated in the appended figures. It is to be appreciated that these figures depict only typical embodiments of the invention and are therefore not to be considered limiting in scope. The invention will be described and explained with additional specificity and detail with the appended figures.

BRIEF DESCRIPTION OF THE DRAWINGS
[0011] The disclosure will be described and explained with additional specificity and detail with the accompanying figures in which:

[0012] FIG. 1 illustrates a flow diagram representing steps involved in a process for synthesizing a biocompatible implant in accordance with an embodiment of the present disclosure;

[0013] FIG. 2 illustrates a flow diagram representing steps involved in a process to fabricate a biocompatible scaffold in accordance with an embodiment of the present disclosure.

[0014] Further, those skilled in the art will appreciate that elements in the figures are illustrated for simplicity and may not have necessarily been drawn to scale. Furthermore, in terms of the construction of the device, one or more components of the device may have been represented in the figures by conventional symbols, and the figures may show only those specific details that are pertinent to understanding the embodiments of the present disclosure so as not to obscure the figures with details that will be readily apparent to those skilled in the art having the benefit of the description herein.

DETAILED DESCRIPTION
[0015] For the purpose of promoting an understanding of the principles of the disclosure, reference will now be made to the embodiment illustrated in the figures and specific language will be used to describe them. It will nevertheless be understood that no limitation of the scope of the disclosure is thereby intended. Such alterations and further modifications in the illustrated system, and such further applications of the principles of the disclosure as would normally occur to those skilled in the art are to be construed as being within the scope of the present disclosure.

[0016] The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such a process or method. Appearances of the phrase "in an embodiment", "in another embodiment" and similar language throughout this specification may, but not necessarily do, all refer to the same embodiment.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure belongs. The kit and examples provided herein are only illustrative and not intended to be limiting.

[0018] In the following specification and the claims, reference will be made to a number of terms, which shall be defined to have the following meanings. The singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise.

[0019] Embodiments of the present disclosure relate to process for synthesizing a biocompatible bone cement. Present disclosure provides a simple, convenient and time saving process for manufacturing the biocompatible bone cement using hybrid nanocomposite for medical and healthcare applications. The biocompatible implant coated with the hybrid nanocomposite enhances the osseointegration and surface properties of the implant, thereby promoting consistent cell proliferation.

[0020] As used herein the term “hybrid nanocomposite” refers to a material created by dispersing inorganic nanoparticles into organic matrix.

[0021] In an embodiment, the process for synthesizing the biocompatible bone cement includes doping nanoparticles of bone cement with gold (Au) nanoparticles using in-situ microwave technique for generating the hybrid nanocomposite. The nanoparticles of bone cement comprise size ranging from 100 to 500 nm and the gold (Au) nanoparticles comprise size less than 50 nm. The hybrid nanocomposite comprises the nanoparticles of bone cement and the gold nanoparticles in a percentage ratio of 0.5: 3. In an embodiment,1 the in-situ microwave technique being carried at a temperature ranging between 80 to 100°C for a duration of 10 to 30 minutes. In such embodiment, the nano bone cement is prepared using polymeric calcium phosphate to enhance the osseointegration and surface properties of the implant.

[0022] As used herein the term “in-situ microwave technique” refers to the process which uses a high-frequency wave to heat the materials to a high temperature in a short time.

[0023] In a further embodiment of the process, the hybrid nanocomposite is further coated onto a metallic implant to obtain the biocompatible implant coated with the hybrid nanocomposite. FIG. 1 illustrates a flow diagram representing steps involved in a process for synthesizing a biocompatible implant in accordance with an embodiment of the present disclosure.

[0024] The process for synthesizing the biocompatible bone cement includes the doping nanoparticles of bone cement with gold (Au) nanoparticles using in-situ microwave technique for generating the hybrid nanocomposite at step 102.

[0025] The hybrid nanocomposite is further coated onto a metallic implant using thermal fusion to obtain the biocompatible implant coated with the hybrid nanocomposite at step 104. The thermal fusion is carried at the temperature ranging from1000 to 1500°C. As used herein the term “thermal fusion” refers to the process which fuses more than one material or substrate via a thermal cycle i.e. the process is carried out at higher temperature than the glass transient temperature of the material.

[0026] In another embodiment of the present disclosure, a biocompatible implant is provided. The biocompatible implant is configured to perform as a substrate that will support the cellular activity including the facilitation of molecular and mechanical signaling systems in order to optimise tissue regeneration without eliciting any undesirable local or systemic responses in the eventual host.

[0027] The biocompatible implant includes, a metallic implant coated with a hybrid nanocomposite. The hybrid nanocomposite comprises nanoparticles of bone cement doped with gold (Au) nanoparticles. The hybrid nanocomposite enhances the osseointegration and surface properties of the implant.

[0028] In yet another embodiment of the present disclosure, a biocompatible bone cement composition is provided. The biocompatible bone cement composition includes nanoparticles of polymeric calcium phosphate doped with noble metal gold (Au) nanoparticles forming a hybrid nanocomposite. The nanoparticles of polymeric calcium phosphate comprise size ranging from 100 to 500nm and the gold nanoparticles comprise size less than 50 nm. The hybrid nanocomposite comprises the polymeric calcium phosphate and the gold nanoparticles in a percentage ratio of 0.5: 3.

[0029] In an embodiment, the biocompatible bone cement composition further includes at least one nano additive, wherein the nano additive comprises collagen and chitosan. The biocompatible bone cement composition enhances the osseointegration and surface properties of the implant. The biocompatible bone cement composition may be used for tissue engineering application. The nano additives collagen, and chitosan also contributes to faster healing time, enhanced bone formation, firmer implant-bone attachment, and enables strong adhesion and good fixation to bone.

[0030] In an embodiment of the present disclosure, a biocompatible scaffold being provided. The biocompatible scaffold may be used as a bone graft and for tissue engineering. The biocompatible scaffold includes nanoparticles of polymeric calcium phosphate doped with noble metal gold (Au) nanoparticles forming a hybrid nanocomposite. The biocompatible scaffold also includes at least one nano additive, wherein the nano additive comprises collagen and/or chitosan. The nanoparticles of polymeric calcium phosphate comprise size ranging from 100 to 500nm and the gold nanoparticles comprise size less than 50 nm. The nano additives collagen, and chitosan also contributes to faster healing time, enhanced bone formation, firmer implant-bone attachment, and enables strong adhesion and good fixation to bone.

[0031] FIG. 2 illustrates a flow diagram representing steps involved in a process for fabricating a biocompatible scaffold in accordance with an embodiment of the present disclosure. In an embodiment, the process for fabricating the biocompatible scaffold is provided. The process includes generating the hybrid nanocomposite at step 202. The process for generating hybrid nanocomposite is same as descried in the step 102. The process further includes mixing a biocompatible binder and one of dispersing agent and additive along with the hybrid nanocomposite to fabricate the biocompatible scaffold at step 204. The biocompatible scaffold is suitable for tissue regeneration.

[0032] The present disclosure provides the simple, convenient and time saving process for synthesizing a biocompatible implant for medical and healthcare applications using in-situ microwave technique and thermal fusion. The process provides the biocompatible implant coated with the hybrid nanocomposite which enhances the osseointegration and surface properties of the implant, thereby promoting consistent cell proliferation.

[0033] While specific language has been used to describe the invention, any limitations arising on account of the same are not intended. As would be apparent to a person skilled in the art, various working modifications may be made to the method in order to implement the inventive concept as taught herein.

[0034] The figures and the foregoing description give examples of embodiments. Those skilled in the art will appreciate that one or more of the described elements may well be combined into a single functional element. Alternatively, certain elements may be split into multiple functional elements. Elements from one embodiment may be added to another embodiment. For example, order of processes described herein may be changed and are not limited to the manner described herein. Moreover, the actions of any flow diagram need not be implemented in the order shown; nor do all of the acts need to be necessarily performed. Also, those acts that are not dependent on other acts may be performed in parallel with the other acts. The scope of embodiments is by no means limited by these specific examples.