Claims:We Claim:

1. A botanical pesticide composition for controlling the pests comprising synergistically effective combination of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized; dry fruit part of Piper nigrumor phytoconstituents such as ß-caryophyllene, 3-carene, eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized; dried seed kernels of Azadirachtaindicaor phytoconstituents such as Azadirachtin, nimbin, nimbolide, salanin or limonoids extracted from botanical source or chemically synthesized; dried seeds of Anonnasquamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomumcamphoraor phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; bud extract of Syzygiumaromaticumor phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized; non-ionic emulsifier, anionic emulsifier and carrier oil at defined concentrations.

2. The botanical pesticide composition according to claim 1, wherein the aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized ranges from 0.001% to 35%; dry fruit part of Piper nigrumor phytoconstituents such as ß-caryophyllene, 3-carene, eugenol, D-limonene or zingiberene extracted from botanical source or chemically synthesized ranges from 0.001% to 35%; dried seed kernels of Azadirachtaindicaor phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized ranges from 0.001% to 40%; dried seeds of Anonnasquamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesizedranges from 0.001% to 25%; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized ranges from 0.001% to 35%; leaf and bark extract of Cinnamomumcamphoraor phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized ranges from 0.001% to 35%; bud extract of Syzygiumaromaticumor phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized ranges from 0.001% to 25%; non-ionic emulsifier ranges from 0.001% to 25%; anionic emulsifier ranges from 0.001% to 25%; and carrier oil ranges from 0.001% to 75% respectively.

3. The botanical pesticide composition according to claim 1, wherein the carrier oil includes but not limited to seed oil of Anethumgraveolens, Pongamaiapinnata oil, Azadirachtaindica oil, castor oil, fish oil, Sesamumindicumoil, Brassica junceaoil and the like.

4. The botanical pesticide composition according to claim 1, wherein the pests includes Thrips, preferably thrips of the order Thysanoptera, which consists of large number of genera and species.
5. The botanical pesticide composition according to claim 1, wherein the said composition is used to control different species of thrips such as but not limited to Western flower thrips (Frankliniellaoccidentalis ), Onion thrips (Thripstabaci ), Melon thrips (Thripspalmi ), Chillithrips and yellow tea thrips (Scirtothripsdorsalis Hood), Black plague thrips (Thrips imagines), European flower thrips (Frankliniellaintonsa ), Tobacco thrips (Frankliniellafusca), Blueberry thrips (Frankliniellavaccinia), and Gladiolus thrips ( Thrips simplex).

6. The botanical pesticide composition according to claim 1, wherein the said composition exhibiting synergism at specific concentration is useful for controlling pests and to protect agriculture,olericulture, floriculture, horticulture, medicinal and economic plants.

7. The botanical pesticide composition according to claim 1, wherein the mode of application of said composition consist of spraying such as foliar sprays and sprays to be applied to the plant shoots.

8. The botanical pesticide composition according to claim 1, wherein the plant-based chemicals including alkaloids, phytosterols, polyphenols, terpenoids, organosulfur compounds, glycosides are not isolated and hence a holistic approach of phytochemicals from plant to control the pests in particular thrips is thus employed.

9. A method of preparation of botanical pesticide composition for controlling the pests comprising:

a. Extracting 0.001 to 35% of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized;0.001 to 35% of dry fruit part of Piper nigrumor phytoconstituentssuch as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized;0.001% to 40% of dried seed kernels of Azadirachtaindicaor phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized;0.001% to 25% of dried seeds of Anonnasquamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomumcamphoraor phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; and 0.001% to 25% of bud extract of Syzygiumaromaticumor phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized by using plurality of extraction techniques;
b. mixing the obtained extracts at given proportion along with 0.001% to 25% of anionic emulsifier and 0.001% to 25% of non-ionic emulsifier;
c. blending the obtained mixture with 0.001% to 75% of carrier oils;
d. stirring the obtained mixture at 200RPM to 800 RPM at 350 C to 650 C in closed mixing vessel made up of stainless steel 316 grade with outer jacket for maintaining temperature, stirrersuch as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring, vessel homogenizer towards the bottom with the speed of 2800 RPM to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make more stable and effective;
e. passing the obtained mixture through in line shear pump with the speed of 1800RPM to 3600 RPM to reduce the particle size to nano, thereby achieving nano emulsion with particle size ranging from 10 to 900 nm;
f. passing the material thus obtained through the filter consisting seven layers of membrane filters with pore size less than 10 µm in order to achieve proper filtration; and
g. Finally, the product thus obtained is used for commercialization and field trails.

10. The method of preparation of botanical pesticide composition according to claim 9, wherein the extraction techniques includes such as not limited to solvent extraction, oleoresin, steam distillation, super critical carbon-dioxide extraction and the like.

11. The method of preparation of botanical pesticide composition according to claim 10, wherein the solvent used in the solvent extraction consist of methanol, Hexane, Acetone, Ethyl acetate, butanol and ethanol.

DATED THIS 05thday of June, 2020

GARGI AMOL PHADATARE (IN/PA/2209)
PATENT AGENT OF APPLICANT
, Description:
FORM – 2

THE PATENTS ACT, 1970
(39 of 1970)

&

THE PATENTS RULES, 2003

COMPLETE SPECIFICATION
(See section 10 and rule 13)

A BOTANICAL PESTICIDE COMPOSITION AND MANUFACTURING METHOD THEREOF

KAY BEE BIO ORGANICS PVT LTD
An Indian Company of
At Post- Sastewadi, Khunte Road,
Taluka:Phaltan, District: Satara, State: Maharashtra,
Country: India, Pin Code: 415523

The following specification particularly describes the invention and the manner in which it is to be performed.

A) TECHNICAL FIELD
[0001] The present invention generally relates to eco-friendly botanical pesticide compounds, formulations, methods of preparation and application and utilities thereof. In particular the present invention relates to botanical pesticides, exhibiting synergism at specific concentration to control the pest and thereby to protect the agriculture, horticulture, olericulture and floricultural crops. Specifically, the present invention relates to botanical pesticides containing no level or substantially lower levels of chemicals. More specifically, the present invention provides for botanical pesticide composition, process of preparing botanical pesticides that are relatively inexpensive to prevent the pests.

B) BACKGROUND OF THE INVENTION
[0002] Pesticides are the cornerstones upon which the pest management practices are based, and are likely to remain so as long as effective and inexpensive chemicals are available. Pests are annoying to humans for a numerous reason. A pesticide is any substance or mixture of substance intended for preventing, destroying, repelling or mitigating any pest. The losses caused by pests in agricultural environments include decreased crop yield, reduced crop quality, and increased harvesting costs.Hence, the current practice to control the destructive pest is the application of pesticides containing synthetic chemical compounds.

[0003] Chemical pesticide due to features such as good drug effectiveness, high service efficiency, remarkable economic benefits, controls pest thus ensures that the agricultural production increases tremendously. Nevertheless, chemical pesticide creates a series of environment and social alarm in a large amount of use practice unreasonably of the mankind, as typical " 3R " problem, which includes pest resistance to insecticide, residue of pesticide and pest renaissance.Moreover, the chemical pesticide also causes chronic toxicity and " three cause " effect, which includes carcinogenic, teratogenesis, mutagenicity.
[0004] Furthermore, the insects have evolved resistance to all types of insecticides including inorganics, DDT, cyclodienes, organophosphates, carbamates, pyrethroids, juvenile hormone analogs, chitin synthesis inhibitors, avermectins, neonicotinoids, and microbials. Resistance occurs in thirteen orders of insects, yet more than 90 percent of the arthropod species with resistant populations are either Diptera (35%), Lepidoptera (15%), Coleoptera (14%), Hemiptera (in the broad sense, 14%), or mites (14%). Agricultural pests account for 59 percent of harmful resistant species while medical and veterinary pests account for 41 percent. Many species have numerous resistant populations, each of which resists many insecticides. Therefore, the statistical analyses suggests that for crop pests, resistance evolves most readily in those with an intermediate number of generations (four to ten) per year that feed either by chewing or by sucking on plant cell contents (Karaagag SU "Insecticide Resistance" In Insecticides - Advances in Integrated Pest Management, Dr. Farzana Perveen (Ed.) 2012; pp469-478).

[0005] In particular, Thrips, is one of the major insect pests and appears from nursery to till the harvest of the crop. They are destructive pests of plants, especially grain crops, fruits and vegetables, and ornamentals. Feeding activities result in plant deformities, scarring, loss of yield, and in some cases, transmission of plant pathogens. Hence, the risk of serious economic losses from thrips makes it important for growers and other agriculturists to be keenly aware to protect the crop from infestation which may lead to use of pesticides. Thrips are members of the order Thysanoptera, which contains a large number of genera and species. Thrips are extremely small insects < 2mm in length and therefore it is unlikely to be detected without magnification. Adults are slender, yellowish, brown in colour, having apically pointed wings. Nymphs are like adults in shape, straw yellow in colour but are wingless and smaller in size. Further, the thrips possesses piercing and sucking mouth parts which cause damage by sucking the contents of individual epidermal cell leading to necrosis of tissue. Tissue colour changes from silvery to brown or black in colour. Moreover, severe infestation of thrips makes the tender leaves and buds brittle, resulting in complete defoliation and total crop loss. In addition, the damaging symptoms caused by thrips includes upward curling of leaves as major symptom which results in boat shaped structure, elongation of petiole, stunted growth, burnt appearance, silvery strakes on pods, deformed pods, reduction in length and size of the pod.

[0006] Furthermore, the thrips can damage plants both directly and indirectly. Direct damage is caused by feeding on the leaves, flowers and fruits, resulting in the deformation, reduced plant growth, altered carbon allocation and thus reduced yield(Welter SC, Rosenheim JA, Johnson MW, Mau RFL, Gusukuma-Minuto LR (1990) Effects of Thrips palmi and western flower thrips (Thysanoptera: Thripidae) on the yield, growth, and carbon allocation pattern in cucumbers. J Econ Entomol 83:2092–2101). The very characteristic direct damage caused by the thrips is the “silvering damage”, which includes piercing the plant cells with their stylet-shaped mouth and sucking out the cell content, leaving silver leaf scars(Chisholm IF, Lewis T (1984) A new look at thrips (Thysanoptera) mouthparts, their action and effects of feeding on plant tissue. Bull Entomol Res 74:663–675). Further, the indirect damage caused by thrips includes the viruses the thrips transmit in order to cause the damage in plants. One of the major viruses the thrips transmit is the tomato spotted wilt virus (TSWV) that causes damage in the pepper plant (German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu Rev Phytopathol 30:315–348).
[0007] Therefore, it is difficult to control the thrips because of thehigh reproductive rate, short life cycle and cryptic behaviour. Hence, the monitoring the thrips population and limiting its growth to prevent an outbreak is therefore important, which can at least partly be accomplished by using cultural, mechanical, physical and biological means. Further, there are reports that the thrips have developed resistance to many molecules of chemical insecticide. In general terms, insecticide resistance occurs when insect populations are subjected to insecticide applications that kill the most susceptible individuals, leaving those that are less susceptible to reproduce and increase the population. Therefore, how quickly an insecticide resistance develops depends on many factors including the type of insecticide used, how often it is used and how capable the insect is in detoxifying the insecticide.

[0008]In view of the foregoing, there is anongoing need for an alternative measure which are safe to the eco-system, capable of bio-degradable, having multi-mechanism action and are less prone to the development of resistance. There is also a need for an alternative pesticide which comprises reduced utilization of synthetic chemical compounds and is eco-friendly, safe for agriculture and humans.

[0009] The above-mentioned shortcomings, disadvantages and problemsare addressed herein and which will be understood by reading and studying thefollowing specification.

C) OBJECTS OF THE INVENTION
[0011] The primary object of the present invention is to provide a synergistic botanical acaricide composition which obviates the limitations as stated above.

[0012] Another object of the present invention is to provide an uniquebotanical acaricide composition consisting of synergistically effective various combination of Mentha piperita or related biomarkers thereof, Cymbopogon citratus or related biomarkers thereof, Brassica napus or related biomarkers thereof, Piper nigrum or related biomarkers thereof, Acorus calamus or related biomarkers thereof, Allium sativum or related biomarkers thereof, adjuvant, non-ionic emulsifier, anionic emulsifier and carrier oil at defined concentrations.

[0013] Yet another object of the present invention is to provide a botanical acaricide composition comprising of various plants extracts exhibiting synergism at specific concentration thereby controls the plant acari and protects agriculture, horticulture, olericulture and floricultural crops.

[0014] Yet another object of the present invention is to provide botanical acaricide, wherein the plant-based chemicals including alkaloids, phytosterols, polyphenols, terpenoids, organosulfur compounds and glycosides are not isolated and have used the holistic approach of phytochemicals from plant to control the plant acari in particular spider mites, red mites, yellow mites more specifically mites from the Tetranychus genera which can be disastrous if not controlled.

[0015] Yet another object of the present invention is to provide botanical acaricide, wherein the botanical extract used as ingredients in formulation are standardized and phyto profile has been explored. Thus, the resultant formulation is been standardized for selected phytochemicals in order to assure quality, as most of the botanical pesticides lacks the standardization.

[0016] Yet another object of the present invention is to provide method of preparation of botanical acaricide and the mode of application of the botanical acaricide.

[0017] Yet another object of the present invention is to provide botanical acaricide comprising natural ingredients such as extracts of plants, used in single or combination or modified to be used as an alternative to synthetic chemical pesticides.

[0018] Yet another object of the present invention is to provide an organic botanical acaricide formulation having a holistic defense approach to control Tetranychidae (mites) family. However, the risk of resistance development is minimized wherein the resistance development is high for synthetic chemical pesticides.

[0019] Yet another object of the present invention is to provide an eco-friendly, non-toxic, bio-degradable and residue free botanical acaricide safe for agriculture and humans.

[0020] These and other objects and advantages of the present inventionwill become readily apparent from the following detailed description taken inconjunction with the accompanying drawings.

D) SUMMARY OF THE INVENTION
[0018] The present invention relates to botanical pesticide composition for controlling the pests specifically thrips. In particular the invention relates to botanical pesticide composition of organic origin prepared by using various botanical extracts from different plant sources, which provide synergistic control against sucking pest specifically thrips. Thrips are members of the order Thysanoptera, which consists of large number of genera and species. The present invention is more particularly related to the botanical pesticide compositions, method of making the botanical pesticide compositions, and methods of applying such compositions to plants to control the pests in particular thrips.

[0019] According to one embodiment of the present invention provides a botanical pesticide composition for controlling pests comprising synergistically effective combination of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized; dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized; dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized; dried seeds of Anonna squamosa or phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized; non-ionic emulsifier, anionic emulsifier and carrier oil at defined concentrations.

[0020] According to one embodiment herein, the botanical pesticide composition comprises by volume approximately from 0.001% to 35% of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized;0.001% to 35% of dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized;0.001% to 40% of dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized;0.001% to 25% of dried seeds of Anonna squamosa or phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; 0.001% to 25% of bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized; 0.001% to 25% of non-ionic emulsifier, 0.001% to 25% of anionic emulsifier and 0.001% to 75% of carrier oilrespectively.

[0021] According to one embodiment of the present invention, the botanical pesticide is used against pests which includes Thrips, preferably thrips of the order Thysanoptera, which consist of large number of genera and species.

[0022] According to one embodiment of the present invention, the botanical pesticide is used against different species of thrips such as but not limited to Western flower thrips (Frankliniella occidentalis ), Onion thrips (Thrips tabaci ), Melon thrips (Thrips palmi ), Chilli thrips and yellow tea thrips (Scirtothrips dorsalis Hood), Black plague thrips (Thrips imagines), European flower thrips (Frankliniella intonsa ), Tobacco thrips (Frankliniella fusca), Blueberry thrips (Frankliniella vaccinia), and Gladiolus thrips ( Thrips simplex).

[0023] According to one embodiment of the present invention, the botanical pesticide exhibiting synergism at specific concentration is useful for controlling pests and to protect agriculture, olericulture, floriculture, horticulture, medicinal and economic plants.

[0024] According to one embodiment of the present invention, the mode of application of botanical pesticide incudes spraying such as foliar sprays and sprays to be applied to the plant shoots.

[0025] According to one embodiment of the present invention, provides a method of preparation of botanical pesticide for controlling the pests comprising extracting 0.001% to 35% of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized;0.001% to 35% of dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized;0.001% to 40% of dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized;0.001% to 25% of dried seeds of Anonna squamosa or phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; 0.001% to 25% of bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized;by using plurality of extraction techniques; mixing the obtained extracts at given proportion along with 0.001% to 25% of anionic emulsifier and 0.001% to 25% of non-ionic emulsifier; followed by blending the obtained mixture with 0.001% to 75% of carrier oils; stirring the obtained mixture at 200 to 800 RPM at 350 C to 650 C in closed mixing vessel made up of stainless steel 316 grade with outer jacket for maintaining temperature, stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring, vessel homogenizer towards the bottom with the speed of 2800 RPM to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make more stable and effective; passing the obtained mixture through in line shear pump with the speed of 1800 RPM to 3600 RPM to reduce the particle size to nano, thereby achieving nano emulsion with particle size ranging from 10 to 900 nm. Further the material obtained is passed through the filter consisting seven layers of membrane filters with pore size less than 10 µm in order to achieve proper filtration. Finally, the product obtained is used for commercialization and field trails.
[0026] According to one embodiment of the present invention, the extraction techniques of the various parts of plurality of plant extracts are carried out by using various extraction techniques such as not limited to solvent extraction, oleoresin, steam distillation and super critical carbon-dioxide extraction. Further, the solvent extraction includes various solvents such as but not limited to methanol, Hexane, Acetone, Ethyl acetate, butanol and ethanol.

[0027] According to one embodiment of the present invention, the carrier oil used in the preparation of botanical pesticide includes but not limited to Seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil and Brassica juncea oil.

[0028] According to one embodiment of the present invention, the botanical pesticide consisting of plant-based chemicals such as phytochemicals which includes alkaloids, phytosterols, polyphenols, terpenoids, organosulfur compounds and glycosides are not isolated. Hence a holistic approach of phytochemicals from plant to control the pests in particular thrips is thus employed. Thrips belonging to the order Thysanoptera, which contains of large number of genera and species, which can be disastrous if not controlled. Hence, the plant extracts used as ingredients in composition are standardized, as well as phyto profile is thus explored. Moreover, the composition is standardized for selected phytochemicals in order to assure the quality, as most of the botanical based pesticides lack the standardization.

[0029] According to one embodiment of the present invention, the in vitro and in vivo trials have been conducted in order to conform the potential outcome of the botanical pesticide to control the thrips attack, in competing with the leading commercial synthetic chemical insectisides which are toxic and not safe to the environment and humans.

[0030] These and other aspects of the embodiments herein will be betterappreciated and understood when considered in conjunction with the followingdescription and the accompanying drawings. It should be understood, however,that the following descriptions, while indicating the preferred embodiments andnumerous specific details thereof, are given by way of an illustration and not of alimitation. Many changes and modifications may be made within the scope ofthe embodiments herein without departing from the spirit thereof, and theembodiments herein include all such modifications.

E) BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The other objects, features, and advantages will occur to thoseskilled in the art from the following description of the preferred embodiment andthe accompanying drawings in which:
[0032] FIG. 1 illustrates a bio-efficacy of botanical pesticide againstthrips under laboratory condition, according to an embodimentof the present invention;
[0033] FIG. 2 illustrates a bio-efficacy of botanical pesticide against thrips infestation on cotton crop under field condition, according to an embodiment of thepresent invention;
[0034] FIG. 3 illustrates an effectiveness of botanical pesticide against thrips infestation on Cotton crop in terms of yield, according to an embodiment of the present invention;
[0035] FIG. 4 illustratesa bio-efficacy of botanical pesticide against thrips infestation on Onion crop under field condition, according to anembodiment of the present invention; and
[0036] FIG. 5 illustrates the effectiveness of botanical pesticide against thrips infestation on Onion crop in terms of yield, according to an embodiment of the presentinvention.

[0037] The specific features of the present invention are shownin some drawings and not in others. This is done for convenience only as eachfeature may be combined with any or all of the other features in accordance withthe present invention.

F) DETAILED DESCRIPTION OF THE INVENTION
[0038] The present invention relates to botanical pesticide composition for controlling the pests specifically thrips. In particular the invention relates to botanical pesticide composition of organic origin prepared by using various botanical extracts from different plant sources, which provide synergistic control against sucking pest specifically thrips. Thrips are members of the order Thysanoptera, which consists of large number of genera and species. The present invention ismore particularly related to the botanical pesticide compositions, method of making the botanical pesticide compositions, and methods of applying such compositions to plants to control the pests in particular thrips.

[0039] Before the present invention is disclosed and described, it is to be understood that this invention is not limited to the particular process steps and materials disclosed herein, as such process steps and materials may vary to some degree. It is also to be understood that the terminology used herein is used for the purpose of describing particular embodiments only and is not intended to be limiting as the scope of the present invention will be limited only by appended claims and equivalents thereof.

[0040] In order to more clearly and concisely describe and point out the subject matter of the claimed invention, the following definitions are provided for specific terms which are used in the following written description.

[0041] The term “pest” when used herein refers to insects, rodents, fungi, bacteria and unwanted plants (weeds) that are harmful to useful plants.

[0042] The term “botanical” when used herein refers to substance obtained from plants.

[0043] The term “pesticide” and “insecticide” as employed in the specification and claims of the application is confined to the means, which adversely affects the existence, growth of the target organism. Such means may comprise a complete killing action, eradicate, arresting growth, inhibition, reducing in number or any combination thereof.

[0044] The term “emulsion” when used herein refers to a stable mixture of two or more immiscible held in suspension.

[0045] The term “emulsifier” when used herein refers to a substance that stabilizes an emulsion.

[0046] The term “control” and “controlling” when used herein refers to alleviating or abrogating a disorder, disease, or condition, or one or more of the symptoms associated with the disorder, disease, or condition; or alleviating or eradicating the cause(s) of the disorder, disease, or condition itself.

[0047] According to one embodiment of the present invention provides a botanical pesticide composition for controlling pests comprising synergistically effective combination of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized; dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized; dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized; non-ionic emulsifier, anionic emulsifier and carrier oil at defined concentrations.

[0048] According to one embodiment herein, the botanical pesticide composition comprises by volume approximately from 0.001% to 35% of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized;0.001% to 35% of dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized;0.001% to 40% of dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized;0.001% to 25% of dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; 0.001% to 25% of bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized; 0.001% to 25% of non-ionic emulsifier, 0.001% to 25% of anionic emulsifier and 0.001% to 75% of carrier oilrespectively.

[0049] According to one embodiment of the present invention, the botanical pesticide is used against pests which includes Thrips, preferably thrips of the order Thysanoptera, which consist of large number of genera and species.

[0050] According to one embodiment of the present invention, the botanical pesticide is used against different species of thrips such as but not limited to Western flower thrips (Frankliniella occidentalis ), Onion thrips (Thrips tabaci ), Melon thrips (Thrips palmi ), Chilli thrips and yellow tea thrips (Scirtothrips dorsalis Hood), Black plague thrips (Thrips imagines), European flower thrips (Frankliniella intonsa ), Tobacco thrips (Frankliniella fusca), Blueberry thrips (Frankliniella vaccinia), and Gladiolus thrips ( Thrips simplex).

[0051] According to one embodiment of the present invention, the botanical pesticide exhibiting synergism at specific concentration is useful for controlling pests and to protect agriculture, olericulture, floriculture, horticulture, medicinal and economic plants.

[0052] According to one embodiment of the present invention, the mode of application of botanical pesticide incudes spraying such as foliar sprays and sprays to be applied to the plant shoots.

[0053] According to one embodiment of the present invention, provides a method of preparation of botanical pesticide for controlling the pests comprising extracting 0.001% to 35% of aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized;0.001% to 35% of dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesized;0.001% to 40% of dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized;0.001% to 25% of dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized; 0.001% to 35% of leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized; 0.001% to 25% of bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized;by using plurality of extraction techniques; mixing the obtained extracts at given proportion along with 0.001% to 25% of anionic emulsifier and 0.001% to 25% of non-ionic emulsifier; followed by blending the obtained mixture with 0.001% to 75% of carrier oils; stirring the obtained mixture at 200 to 800 RPM at 350 C to 650 C in closed mixing vessel made up of stainless steel 316 grade with outer jacket for maintaining temperature, stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring, vessel homogenizer towards the bottom with the speed of 2800 RPM to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make more stable and effective; passing the obtained mixture through in line shear pump with the speed of 1800 RPM to 3600 RPM to reduce the particle size to nano, thereby achieving nano emulsion with particle size ranging from 10 to 900 nm. Further the material obtained is passed through the filter consisting seven layers of membrane filters with pore size less than 10 µm in order to achieve proper filtration. Finally, the product obtained is used for commercialization and field trails.

[0054] According to one embodiment of the present invention, the extraction techniques of the various parts of plurality of plant extracts are carried out by using various extraction techniques such as not limited to solvent extraction, oleoresin, steam distillation and super critical carbon-dioxide extraction. Further, the solvent extraction includes various solvents such as but not limited to methanol, Hexane, Acetone, Ethyl acetate, butanol and ethanol.

[0055] According to one embodiment of the present invention, the carrier oil used in the preparation of botanical pesticide includes but not limited to Seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil and Brassica juncea oil.

[0056] According to one embodiment of the present invention, the botanical pesticide consisting of plant based chemicals such as phytochemicals which includes alkaloids, phytosterols, polyphenols, terpenoids, organosulfur compounds and glycosides are not isolated. Hence a holistic approach of phytochemicals from plant to control the pests in particular thrips is thus employed. Thrips belonging to the order Thysanoptera, which contains of large number of genera and species, which can be disastrous if not controlled. Hence, the plant extracts used as ingredients in composition are standardized, as well as phyto profile is thus explored. Moreover, the composition is standardized for selected phytochemicals in order to assure the quality, as most of the botanical based pesticides lack the standardization.

[0057] According to one embodiment of the present invention, the in vitro and in vivo trials have been conducted in order to conform the potential outcome of the botanical pesticide to control the thrips attack, in competing with the leading commercial synthetic chemical insecticides which are toxic and not safe to the environment and humans.

[0058] According to one embodiment of the present invention, the FIG.1 illustrates a bio-efficacy of botanical pesticide againstthrips under laboratory conditions. Further the Table 1 provides the percentage of mortality of thrips against botanical pesticide vs various pesticides available in the market. The Table 1 provides that the botanical pesticide offers 78% to 90% mortality after 48 hours to 72 hoursof treatment at dosage of 0.5ml and 1 ml respectively when compared to other pesticides available in the market.

Table 1: In-vitro efficacy of botanical pesticide against Thrips
Treat. No. Treatment Dose (per L) Per cent Mortality
After 48 hrs. After 72 hrs.
T1 Botanical pesticide 25% EC 0.5 ml 78 85
T2 Botanical pesticide 25% EC 1 ml 85 90
T3 Acetamiprid 20% SP 1 g 62 67
T4 Cypermethrin 10% EC 1.5 ml 72 77
T5 Deltamethrin 11% EC 2 ml 65 69
T6 Spinosad 45% SC 0.3 ml 75 79
T7 Lambda Cyhalothrin 5% EC 1 ml 68 73
T8 Profenophos 50% EC 1 ml 65 71
T9 Cyantraniliprole 10.26% EC 1.8 ml 68 74
T10 Profenophos 40% + Cypermethrin 4% EC 1 ml 70 75
T11 Emamectin Benzoate 5% SG 0.5 g 69 73
T12 Control (Water spray) - 0 0

[0059] According to one embodiment of the present invention, the FIG.2 illustrates a bio-efficacy of botanical pesticide against thrips infestation on Cotton crop under field condition. Further the Table 2 provides the percentage mortality of thrips against botanical pesticide vs various pesticides available in the market. The Table 2 provides that the botanical pesticide offers 72% to 78% of thrips mortality after 3days of treatment at dosage of 1 ml to 1.5 ml respectively; 68% to 76% of thrips mortality after 5 days of treatment at dosage and 1 ml to 1.5 ml respectively; and 68% to 71% of thrips mortality after 7 days of treatment at dosage 1 ml to 1.5 ml respectively when compared to other pesticides available in the market.

[0060] According to one embodiment of the present invention, the FIG. 3 illustrates an effectiveness of botanical pesticide against thrips infestation on Cotton crop in terms of yield. Further the Table 2 provides the effectiveness of botanical pesticide against thrips infestation on Cotton crop in terms of yield. The Table 2 provides that the botanical pesticide provides 12.4 to 12.85 quintals/ha-1 against various pesticides available in the market.
Table 2: In-vivo bio-efficacy of botanical pesticide against thrips infestation on Cotton crop
Treat. No. Treatment Dose (per L) Pre-count Per cent Mortality Yield
(quintals/ ha-1)
3rd DAS 5th DAS 7th DAS
T1 Botanical pesticide 25% EC 1 ml 23 72 68 68 12.4
T2 Botanical pesticide 25% EC 1.5 ml 22 78 76 71 12.85
T3 Acetamiprid 20% SP 1 g 24 56 54 50 6.95
T4 Cypermethrin 10% EC 1.5 ml 21 63 60 58 8.75
T5 Deltamethrin 11% EC 2 ml 23 52 52 50 6.85
T6 Fipronil 5% SC 1 ml 22 53 50 48 5.95
T7 Imidachloprid 70% WG 0.17 g 25 50 48 46 5.35
T8 Spinosad 45% SC 0.3 ml 21 64 61 58 7.35
T9 Lambda Cyhalothrin 5% EC 1 ml 23 56 52 54 6.75
T10 Profenophos 50% EC 1 ml 24 52 50 48 6.05
T11 Cyantraniliprole 10.26% EC 1.8 ml 21 52 50 48 5.95
T12 Profenophos 40% + Cypermethrin 4% EC 1 ml 23 54 52 45 5.15
T13 Emamectin Benzoate 5% SG 0.5 g 22 62 59 56 7.45
T14 Control (Water spray) - 23 0 0 0 4.85
* Days after spray

[0061] According to one embodiment of the present invention, the FIG.4 illustrates a bio-efficacy of botanical pesticide against thrips infestation on Onion crop under field condition. Further the Table 3 provides the percentage mortality of thrips against botanical pesticide vs various pesticides available in the market. The Table 3 provides that the botanical pesticide offers 77% to 81% of thrips mortality after 3days of treatment at dosage of 1 ml to 1.5 ml respectively; 74% to 78% of thrips mortality after 5 days of treatment at dosage and 1 ml to 1.5 ml respectively; and 69% to 73% of thrips mortality after 7 days of treatment at dosage 1 ml to 1.5 ml respectively when compared to other pesticides available in the market.

[0062] According to one embodiment of the present invention, the FIG. 5 illustrates an effectiveness of botanical pesticide against thrips infestation on Onion crop in terms of yield. Further the Table 3 provides the effectiveness of botanical pesticide against thrips infestation on Onion crop in terms of yield. The Table 3 provides that the botanical pesticide provides 33.15 to 35.05quintals/ha-1 against various pesticides available in the market.
Table 3: In-vivo bio-efficacy of botanical pesticide against Thrips infestation on Onion crop under field condition
Treat. No. Treatment Dose (per L) Pre-count Per cent Mortality Yield
(t/ ha-1)
3rd DAS 5th DAS 7th DAS
T1 Botanical pesticide 25% EC 1 ml 23 77 74 69 33.15
T2 Botanical pesticide 25% EC 1.5 ml 22 81 78 73 35.05
T3 Acetamiprid 20% SP 1 g 24 57 54 50 16.65
T4 Cypermethrin 10% EC 1.5 ml 21 67 62 58 25.35
T5 Deltamethrin 11% EC 2 ml 23 54 51 47 17.15
T6 Profenophos 50% EC 1 ml 22 52 48 46 16.45
T7 Cyantraniliprole 10.26% EC 1.8 ml 25 53 48 45 16.35
T8 Spinosad 45% SC 0.3 ml 21 69 64 59 25.65
T9 Lambda Cyhalothrin 5% EC 1 ml 23 61 57 52 22.75
T10 Profenophos 40% + Cypermethrin 4% EC 1 ml 24 62 58 53 23.65
T11 Emamectin Benzoate 5% SG 0.5 g 21 58 54 51 16.35
T12 Fipronil 5% SC 1 ml 23 54 52 45 17.05
T13 Imidachloprid 70% WG 0.17 g 22 59 56 52 17.25
T14 Control (Water spray) - 23 0 0 0 13.15

* Days after spray

[0063] The present invention may be more clearly understood with reference to the following examples of the invention which are given by way of example only. One has to consider that the following examples are included to demonstrate certain non-limiting aspects of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention. However, those of skilled in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
[0064] Example 1
Representative botanical pesticide composition 1 and the corresponding method of preparation
An exemplary composition 1 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Piper nigrum 5 0.001 to 35 %
2. Azadirachta Indica 5 0.001 to 40 %
3. Anonna squamosa 6 0.001 to 25 %
4. Cinnamomum cassia 7.5 0.001 to 35 %
5. Cinnamomum camphora 7 0.001 to 35 %
6. Syzygium aromaticum 2 0.001 to 25 %
7. Non-Ionic Emulsifier 7 0.001 to 25 %
8. Anionic Emulsifier 5 0.001 to 25 %
9. Carrier oil 55.5 0.001 to 75 %

Method of preparation of botanical pesticide composition 1 comprises the following steps:
1. Extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0065] Example 2
Representative botanical pesticide composition 2 and the corresponding method of preparation

Sr No. Ingredient Actual Percent Percent Range
1. Piper nigrum 5 0.001 to 35 %
2. Azadirachta Indica 5 0.001 to 40 %
3. Anonna squamosa 7 0.001 to 25 %
4. Cinnamomum camphora 7 0.001 to 35 %
5. Syzygium aromaticum 7 0.001 to 25 %
6. Non-Ionic Emulsifier 7 0.001 to 25 %
7. Anionic Emulsifier 5 0.001 to 25 %
8. Carrier oil 57 0.001 to 75 %

Method of preparation of botanical pesticide composition 2 comprises the following steps:
1. Extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0066] Example 3
Representative botanical pesticide composition 3 and the corresponding method of preparation
An exemplary composition 3 in accordance with the principle of the present invention comprises:

Sr No. Ingredient Actual Percent Percent Range
1. Thymus vulgaris 4 0.00 1 to 35 %
2. Piper nigrum 4 0.001 to 35 %
3. Azadirachta Indica 6 0.001 to 40 %
4. Anonna squamosa 8 0.001 to 25 %
5. Cinnamomum camphora 10 0.001 to 35 %
6. Non-Ionic Emulsifier 7 0.001 to 25 %
7. Anionic Emulsifier 5 0.001 to 25 %
8. Carrier oil 56 0.001 to 75 %

Method of preparation of botanical pesticide composition 3 comprises the following steps:
1. Extracting aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized of percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35%; dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; and leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0067] Example 4
Representative botanical pesticide composition 4 and the corresponding method of preparation
An exemplary composition 4 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Thymus vulgaris 2 0.00 1 to 35 %
2. Piper nigrum 3 0.001 to 35 %
3. Azadirachta Indica 6 0.001 to 40 %
4. Anonna squamosa 6 0.001 to 25 %
5. Cinnamomum cassia 7 0.001 to 35 %
6. Syzygium aromaticum 7 0.001 to 25 %
7. Non-Ionic Emulsifier 7 0.001 to 25 %
8. Anionic Emulsifier 5 0.001 to 25 %
9. Carrier oil 57 0.001 to 75 %

Method of preparation of botanical pesticide composition 4 comprises the following steps:
1. Extracting aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized of percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35%; dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0068] Example 5
Representative botanical pesticide composition 5 and the corresponding method of preparation
An exemplary composition 5 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Azadirachta Indica 5 0.001 to 40 %
2. Anonna squamosa 4 0.001 to 25 %
3. Cinnamomum camphora 4 0.001 to 35 %
4. Syzygium aromaticum 2 0.001 to 25 %
5. Non-Ionic Emulsifier 7 0.001 to 25 %
6. Anionic Emulsifier 5 0.001 to 25 %
7. Carrier oil 55.5 0.001 to 75 %

Method of preparation of botanical pesticide composition 5 comprises the following steps:
1. Extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting the dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0069] Example 6
Representative botanical pesticide composition 6 and the corresponding method of preparation
An exemplary composition 6 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Piper nigrum 2.5 0.001 to 35 %
2. Azadirachta Indica 2.5 0.001 to 40 %
3. Anonna squamosa 4 0.001 to 25 %
4. Cinnamomum cassia 4 0.001 to 35 %
5. Syzygium aromaticum 6 0.001 to 25 %
6. Non-Ionic Emulsifier 7 0.001 to 25 %
7. Anionic Emulsifier 5 0.001 to 25 %
8. Carrier oil 69 0.001 to 75 %

Method of preparation of botanical pesticide composition 6 comprises the following steps:
1. Extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.

[0070] Example 7
Representative botanical pesticide composition 7 and the corresponding method of preparation
An exemplary composition 7 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Piper nigrum 5 0.001 to 35 %
2. Anonna squamosa 8 0.001 to 25 %
3. Cinnamomum camphora 10 0.001 to 35 %
4. Syzygium aromaticum 10 0.001 to 25 %
5. Non-Ionic Emulsifier 7 0.001 to 25 %
6. Anionic Emulsifier 5 0.001 to 25 %
7. Carrier oil 55 0.001 to 75 %

Method of preparation of botanical pesticide composition 7 comprises the following steps:
1. Extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extracts with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.
[0071] Example 8
Representative botanical pesticide composition 8 and the corresponding method of preparation
An exemplary composition 8 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Thymus vulgaris 3 0.00 1 to 35 %
2. Cinnamomum cassia 5 0.001 to 35 %
3. Cinnamomum camphora 15 0.001 to 35 %
4. Syzygium aromaticum 15 0.001 to 25 %
5. Non-Ionic Emulsifier 7 0.001 to 25 %
6. Anionic Emulsifier 5 0.001 to 25 %
7. Carrier oil 50 0.001 to 75 %

Method of preparation of botanical pesticide composition 8 comprises the following steps:
1. Extracting aerial parts of Thymus vulgaris or phytoconstituents such as thymol, carvacrol, cymene, terpinene or linalool extracted from botanical source or chemically synthesized of percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extract with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.
[0072] Example 9
Representative botanical pesticide composition 9 and the corresponding method of preparation
An exemplary composition 9 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Piper nigrum 3 0.001 to 35 %
2. Azadirachta Indica 3 0.001 to 40 %
3. Cinnamomum camphora 5 0.001 to 35 %
4. Syzygium aromaticum 10 0.001 to 25 %
5. Non-Ionic Emulsifier 7 0.001 to 25 %
6. Anionic Emulsifier 5 0.001 to 25 %
7. Carrier oil 67 0.001 to 75 %

Method of preparation of botanical pesticide composition 9 comprises the following steps:
1. Extracting dry fruit part of Piper nigrum or phytoconstituents such as ß-caryophyllene, 3-carene , eugenol , D-limonene or zingiberene extracted from botanical source or chemically synthesizedof percentage range about 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40%; leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and bud extract of Syzygium aromaticum or phytoconstituents such as Eugenol, caryophyllene, eugenyl acetate or chavicol extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extract with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.
[0073] Example 10
Representative botanical pesticide composition 10 and the corresponding method of preparation
An exemplary composition 10 in accordance with the principle of the present invention comprises
Sr No. Ingredient Actual Percent Percent Range
1. Azadirachta Indica 6 0.001 to 40 %
2. Anonna squamosa 12 0.001 to 25 %
3. Cinnamomum cassia 12 0.001 to 35 %
4. Cinnamomum camphora 15 0.001 to 35 %
5. Non-Ionic Emulsifier 7 0.001 to 25 %
6. Anionic Emulsifier 5 0.001 to 25 %
7. Carrier oil 43 0.001 to 75 %

Method of preparation of botanical pesticide composition 10 comprises the following steps:
1. Extracting dried seed kernels of Azadirachta indica or phytoconstituents such as Azadirachtin, nimbin, nimbolide , salanin or limonoids extracted from botanical source or chemically synthesized of percentage range about 0.001% to 40% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
2. Similarly extracting dried seeds of Anonna squamosaor phytoconstituents such as annonacin extracted from botanical source or chemically synthesized of percentage range of 0.001% to 25%; leaf and bark extract of Cinnamomum cassia or phytoconstituents such as cinnamaldehyde, cinnamyl acetate, benzaldehyde, camphor or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35%; and leaf and bark extract of Cinnamomum camphora or phytoconstituents such as D-camphor, 1,8-cineole, terpineol, limonene or camphene extracted from botanical source or chemically synthesized of percentage range of 0.001% to 35% by using various extraction techniques such as but not limited to solvent (methanol, hexane, acetone, ethyl acetate, butanol, ethanol) extract, oleoresin, steam distillation or super critical carbon dioxide extraction.
3. Mixing the obtained extract with anionic emulsifier in percentage range of 0.001% to 25% and non-ionic emulsifier in percentage range 0.001% to 25% respectively.
4. Further blending the obtained mixture with carrier oil such as but limited to seed oil of Anethum graveolens, Pongamaia pinnata oil, Azadirachta indica oil, castor oil, fish oil, Sesamum indicum oil or Brassica juncea oil in a percentage range 0.001% to 75%.
5. Stirring the obtained mixture at 200RPM to 800RPM speed and subjecting to 350C to 650C temperature in closed mixing vessel, wherein the vessel is made up of stainless steel 316 grade consisting of outer jacket for maintaining temperature, connected with stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer or universal stirrer for continuous stirring of the mixture. Further the vessel also comprises homogenizer towards the bottom which runs at a speed of 2800 RPM is attached to reduce the particle size under very high pressure, sheer, turbulence, acceleration and impact to make the particles more stable and effective.
6. Further the obtained mixture is then subjected to line shear pump working at a speed of 1800 RPM to 3600 RPM in order to reduce the particle size of the mixture to nano, so as to achieve the nano emulsion with particle size ranging from 10 nm to 900 nm.
7. After shearing the material is then passed through the filter consisting of seven layers of membrane filters with pore size less than 10 µm to achieve proper filtration.
8. Finally, the product is recovered and is used for commercialization and field trails.
[0074] From the foregoing discussion, it is apparent that the botanical pesticide composition prepared from the various plant extracts have a holistic defense approach to control the pests in particular thrips, members of the order Thysanoptera which consists of large number of genera and species which can be disastrous if not controlled. However, the risk of resistance development of botanical pesticide against the thrips is very low when compared to the synthetic chemical insecticides. Therefore, the botanical pesticide composition thus prepared is safe and non-toxic for the environment, bio-degradable and residue free.
[0075] The foregoing description of the specific embodiments will sofully reveal the general nature of the embodiments herein that others can, byapplying current knowledge, readily modify and/or adapt for various applicationssuch specific embodiments without departing from the generic concept, and,therefore, such adaptations and modifications should and are intended to becomprehended within the meaning and range of equivalents of the disclosedembodiments.

[0076] It is to be understood that the phraseology or terminologyemployed herein is for the purpose of description and not of limitation.Therefore, while the embodiments herein have been described in terms ofpreferred embodiments, those skilled in the art will recognize that theembodiments herein can be practiced with modifications.