CLIAMS:1. A method of preparing a cell free bacterial supernatant for cosmetic inhibition of oily skin, said method comprising of:
a. obtaining at least one bacterial species and culturing in a first culture medium;
b. removing bacterial cells from said first culture medium to obtain said cell free bacterial supernatant,
wherein said first culture medium comprises of bacterial growth media, and at least one prebiotic fiber selected from the group consisting of pectin, glucomannan, arabinogalactan, Konjac gums, carrube gums, guar gums, PHGG, or tara gums), fructo-oligosaccharide (“FOS”), soy -oligosaccharide, inulin, pectin or pectic polysaccharide, xanthan gum, beta-glucan, galactomannans, and combinations thereof.
2. The method as claimed in claim 1, wherein said at least one bacterial species is selected from the group consisting of Lactobacillus casei, Lactobacillus fermentum, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus curvatus, Lactobacillus bulgaricus, Lactobacillus grasseri, Lactobacillus caveasicus, Lactobacillus helveticus, Lactobacillus lactis, Lactobacillus salivarius, Lactobacillus rhamnosus, Lactobacillus brevis, Lactobacillus leichmanni, Lactobacillus plantarum, Lactobacillus cellobiosus, Lactobacillus buchneri, Bifidobacterium laterosporus, Bacillus breve, Bacillus subtilus, Lactobacillus sporogenes, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus faecium, Bifidobacterium adolescentis, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermophilum, Bifidobacterium animalis, Bifidobacterium bifidum, Lactococcus lactis cremoris, Streptococcus diacetylactis and Sterptococcus intermedius, and combinations thereof.
3. The method as claimed in claim 1, wherein said at least one prebiotic fiber is a combination of pectin and glucomannan.
4. The method as claimed in claim 3, wherein pectin to glucomannan w/w ratio in said at least one prebiotic combination is in the range of 1:10 to 10:1.
5. The method as claimed in claim 1, wherein said at least one prebiotic fiber weight percentage in said first culture medium is in the range of 0.01-5%.
6. The method as claimed in claim 1, wherein said culturing is carried out at 35-40 ºC for 8-48 hours under constant shaking conditions.
7. A cell free bacterial supernatant for cosmetic inhibition of oily skin, said cell free bacterial supernatant prepared by a method as claimed in any of the claims 1-6.
8. A method of cosmetically inhibiting oily skin, said method comprising of:
a. obtaining a cell free bacterial supernatant as claimed in claim 7; and
b. contacting said cell free bacterial supernatant with skin,
wherein said method cosmetically inhibits oily skin.
9. A cell free bacterial supernatant as claimed in claim 7, prepared by a method as claimed in any of the claims 1-6 for use in inhibition of lipogenesis.
Dated this 24 day of March 2015
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