1. A circuit (102) for detecting fluorescence signals in multiplex Polymerase Chain Reactions
(PCR), comprising:
a plurality of light sources (112) configured to generate light signals;
a photo detector (106) configured to detect the light signals;
one or more oscillators (114) configured to generate a reference waveform of a defined
10 frequency;
one or more filters (116) configured to:
modulate the reference waveform to generate a modulated reference waveform;
and
wherein the reference waveform and the modulated reference waveform are selectively
15 coupled with the light signals;
and
a detector circuit (108) configured to demodulate the light signals detected by the
photo detector (106) using the reference waveform and the modulated reference waveform.
20 2. The circuit (102) as claimed in claim 1, wherein the one or more filters (116) are configured
to phase modulate or frequency modulate the reference waveform, wherein the phase
modulated reference waveform is a quadrature phase waveform.
3. The circuit (102) as claimed in claim 1, wherein each of the plurality of light sources (112)
25 are configured to generate different wavelength of light signal, wherein the reference waveform
and modulated reference waveform are selectively coupled with the light signals.
4. The circuit (102) as claimed in claim 1, wherein the photo detector (106) is configured to
detect the light signals refracted by one or more samples, upon passing the light signals
30 generated from the plurality of light sources 112, through the one or more samples.
5. The circuit (102) as claimed in claim 1, wherein the detector circuit (108) is further
configured to resolves the light signals detected by the photo detector (106) into different
fluorescence signals based on the reference waveform and the modulated reference waveform.
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20
6. A device (100) for detecting fluorescence signals in multiplex Polymerase Chain
Reactions (PCR), the device (100) comprising;
a sample holder (110);
one or more bays, wherein each of the one or more bays comprising one or more light
5 sources (112) for generating light signals; and
a circuit (102) comprising:
a plurality of light sources (112) configured to generate light signals;
a photo detector (106) configured to detect the light signals;
one or more oscillators (114) configured to generate a reference waveform of a
10 defined frequency;
one or more filters (116) configured to:
modulate a reference waveform to generate modulated reference
waveform; and
wherein the reference waveform and the modulated reference waveform are
15 selectively coupled with the light signals;
and
a detector circuit (108) configured to demodulate the light signals detected by
the photo detector (106) using the reference waveform and the modulated reference waveform;
and
20 wherein the circuit (102) is connected with the one or more bays.
7. The device as claimed in claim 6, further comprising a computing system (104) for analysing
demodulated light signals received from the circuit (102).
25 8. The device as claimed in 6, wherein the sample holder (110) is placed between the plurality
of light sources (112) and a photo detector (106) of the circuit (102).
9. A method for detecting fluorescence signals in multiplex Polymerase Chain Reactions
(PCR), the method comprises;
30 generating, by a plurality of light sources (112), light signals;
generating, by one or more oscillators (114), a reference waveform of a defined
frequency;
21
modulating, by one or more filters (116), a reference waveform to generate modulated
reference waveform;
wherein the reference waveform and the modulated reference waveform are
selectively coupled with the light signals;
5 detecting, by a photo detector (106), the light signals; and
demodulating, by a detector circuit (108), the light signals detected by the photo detector
(106) using the reference waveform and the modulated reference waveform.
10. The method as claimed in claim 9, further modulates the reference waveform to phase
10 reference modulated waveform or frequency modulate reference waveform, wherein the phase
modulated reference waveform is a quadrature phase waveform.