DESC:] In describing and claiming the invention, the following terminology will be used in accordance with the definitions set forth below. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are described herein. As used herein, each of the following terms has the meaning associated with it in this section. Specific and preferred values listed below for individual process parameters, substituents, and ranges are for illustration only; they do not exclude other defined values or other values falling within the preferred defined ranges.
[015] As used herein, the singular forms "a," "an," and "the" include plural reference unless the context clearly dictates otherwise.
[016] The terms “preferred” and “preferably” refer to embodiments of the invention that may afford certain benefits, under certain circumstances. However, other embodiments may also be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, and is not intended to exclude other embodiments from the scope of the invention
[017] As used herein, the term “disinfect” shall mean the elimination of many or all pathogenic microorganisms on surfaces with the exception of bacterial endospores.
[018] As used herein, the term “sanitize” shall mean the reduction of contaminants in the inanimate environment to levels considered safe.
[019] The term “surfactant”, as used herein refers to a substance that reduces surface tension when dissolved in water or water solutions, or that reduces interfacial tension between two liquids, or between a liquid and a solid.
[020] The present invention is directed to composition for disinfectant and cleaning spray comprises of a high stable bleach product, pH adjuster, a buffering agent, a primary surfactant, a secondary surfactant, at least one fragrance ingredient, dispersion of nano-particle suspension and water. The present innovation overcomes the problems of the prior art and also incorporates nano particles to enhance the disinfecting ability and long lasting fragrance.
[021] According to an embodiment of the invention, there is provided a cleaning disinfectant composition for spraying on surfaces, said composition comprising:
- a hypochlorite of an alkali metal or a hypochlorite of an alkaline earth metal in a range of 0.25 to 5% by weight,
- a primary surfactant in a range of 0.1 to 0.5% by weight,
- a secondary surfactant in a range of 0.1 to 0.5% by weight,
- a nanoparticle suspension in a range of 0.05 to 0.2% by weight,
- a pH booster in a range of 0.1 to 0.5% by weight, and
- a buffering agent in a range of 0.1 to 0.5% by weight,
of the total composition in water, wherein,
the primary surfactant is an anionic organic surfactant or a cationic surfactant having cleaning ability, and the secondary surfactant is a non-ionic or amphoteric organic surfactant having foam boosting ability.
[022] The hypochlorite of an alkali metal or a hypochlorite of an alkaline earth metal is a high stable bleach product which is preferably sodium hypochlorite, calcium hypochlorite, lithium hypochlorite or a combination thereof. The sodium hypochlorite used in the composition of the invention is commercially available. In an embodiment, the amount of sodium hypochlorite is in the range of 2- 5%, more preferably 3% to the total weight of the composition.
[023] The compositions according to the present invention includes a primary surfactant and a secondary surfactant. These surfactants may include anionic, non-ionic, cationic or combinations thereof. Non-limiting examples of surfactants are discussed below.
[024] Suitable secondary surfactants i.e. non-ionic or amphoteric surfactants include, but are not limited to, ethoxylated alcohols, ethoxylated phenols, ethoxylated fatty acid esters, ethoxylated glycerol fatty acid esters, sorbitan fatty acid esters, glycerol fatty acid esters, coconut monoethanolamide ethoxylates, ethoxylated tall oil, ethoxylated polypropylene glycol, fatty acid alkanolamides such as coconut mono- and diethanolamide, amine oxides such as lauryl amine oxide, myrsityl amine oxide, amido-amine oxides like lauryl di-methyl amidoamine oxide, alkylamidopropylamine N-oxide, n-alkyl pyrrolidones, alkyl polysaccharides such as sucrose esters and alkyl polyglycosides, alkyl phenol ethoxylates, ethoxylated castor oil, fatty acid ethoxylates, fatty amine ethoxylates, polyglycerol fatty acid esters, and alkyl adducts of ethylene-oxide-propylene oxide copolymers, betaines such as cocamido propyl betaine, and combinations thereof. In one embodiment, the nonionic surfactant comprises an ethoxylated alcohol such as a C12-C15 ethoxylated alcohol available commercially. In another embodiment, the surfactant comprises an alkyl polyglycoside like a mixture of D-glucopyranoside or a similar C10-C16 alkyl oligomer and D-glucose, or a D glucopyranoside or a similar C10-C16 alkyl oligomer. In another embodiment, the surfactant comprises an alkyl polyglycoside like a mixture comprising a C10-C16 alkyl polyglycoside and a C8-C10 alcohol ethyoxylate propoxylate plus decene epoxide. In another embodiment, the surfactant comprises an alkyl polyglycoside like a mixture comprising a C10-C16 alkyl polyglycoside, and reaction products of epichlorohydrin and isodecyl alcohol-4EO. In yet another embodiment, the nonionic surfactant comprises an iso-C10-ethylene oxide-propylene oxide copolymer adduct. Another suitable nonionic surfactant is an ethoxylated nonylphenol available as a mixture with another surfactant.
[025] Suitable primary anionic surfactants include, but are not limited to, linear and/or branched chain alkylbenzene sulfonates, alkyl sulfates, ether sulfates, secondary alkyl sulfates, a-olefin sulfonates, phosphate esters, sulfosuccinates, isethionates, carboxylates, and combinations comprising one or more of the foregoing anionic surfactants. Specific anionic surfactants include, for example, sodium lauryl sulfate, sodium lauryl ether sulfate, triethanolamine lauryl sulfate, magnesium lauryl sulfate, sulfosuccinate esters, ammonium lauryl sulfate, alkyl sulfonates, sodium lauryl sulfate, sodium alpha olefin sulfonates, alkyl sulfates, sulfated alcohol ethoxylates, sulfated alkyl phenol ethoxylates, sodium xylene sulfonate, alkylbenzene sulfonates such as triethanolamine dodecylbenzene sulfonate, sodium dodecylbenzene sulfonate, calcium dodecylbenzene sulfonate, xylene sulfonic acid, dodecylbenzene sulfonic acid, N alkoyl sarcosinates such as sodium lauroyl sarcosinate, dialkylsulfosuccinates, N alkoyl sarcosines such as lauroyl sarcosine, alkyl ether carboxylates, soaps including sodium, potassium, magnesium, calcium, alkanolamine, and amine soaps, and combinations comprising one or more of the foregoing anionic surfactants. In one embodiment, the anionic surfactant is sodium lauryl sulfate available commercially.
[026] Suitable primary cationic surfactants include, but are not limited to, quaternary ammonium compounds including alkyl dimethyl benzyl ammonium chloride, dialkyl dimethyl ammonium chloride, alkyl trimethyl ammonium chloride or bromide, salts of organic or inorganic acids with fatty amines, fatty amine ethoxylates, and combinations comprising one or more of the foregoing cationic surfactants.
[027] In one embodiment, preferably said primary surfactant is sodium lauryl sarcosinate, in the range of 0.1 to 0.5 wt%. In one embodiment, preferably, said secondary surfactant is lauryl amine oxide in the range of 0.1 – 0.5 wt% .
[028] In one embodiment, the composition comprises of at least one fragrance imparting ingredient. In one embodiment, the fragrance may mask the pungent order of chlorine and/ or modify an unpleasant odour to a pleasant odour. The fragrance may be natural or synthetic, which is volatile and compatible with hypohalites. Natural fragrance may comprise, but is not limited to, essential oils derived from lemon, lime, eucalyptus, Pine, Jasmine, Water Lily, Tea-tree, etc. Synthetic sources may comprise camphor, Menthol, 2-Octanol etc. The concentration of fragrance may be 0.1 to 1% or even 0.001 to 2.5 %, More preferably 0.05 to 2.5 % and most preferably 0.1 to 2.5 %.
[029] Suitable fragrances include, but not limited to, for example, anethol, methyl heptine carbonate, ethyl aceto acetate, para cymene, nerol, decyl aldehyde, para cresol, methyl phenyl carbinyl acetate, undecylenic aldehyde, undecyl aldehyde, 2,6-nonadienal, nonyl aldehyde, octyl aldehyde, phenyl acetaldehyde, anisic aldehyde, benzyl acetone, ethyl-2-methyl butyrate, damascenone, damascone alpha, damascone beta, flor acetate, frutene, fructone, herbavert, iso cyclo citral, methyl isobutenyl tetrahydro pyran, isopropyl quinoline, 2,6-nonadien-1-ol, 2-methoxy-3-(2-methylpropyl)- pyrazine, methyl octine carbonate, tridecene-2-nitrile, allyl amyl glycolate, cyclogalbanate, cyclal C, melonal, gamma nonalactone, cis 1,3-oxathiane-2-methyl-4- propyl, benzaldehyde, benzyl acetate, camphor, camphene, carvone, borneol, bornyl acetate, decyl alcohol, eucalyptol, linalool, hexyl acetate, iso-amyl acetate, thymol, carvacrol, limonene, menthol, iso-amyl alcohol, phenyl ethyl alcohol, alpha pinene, a terpineol, citronellol, alpha thujone, benzyl alcohol, beta gamma hexenol, dimethyl benzyl carbinol, phenyl ethyl dimethyl carbinol, adoxal, allyl cyclohexane propionate, beta pinene, citral, citronellyl acetate, citronellal nitrile, dihydro myrcenol, geraniol, geranyl acetate, geranyl nitrile, hydroquinone dimethyl ether, hydroxycitronellal, linalyl acetate, phenyl acetaldehyde dimethyl acetal, phenyl propyl alcohol, prenyl acetate, triplal, tetrahydrolinalool, verdox, cis-3-hexenyl acetate, ethyl methyl phenyl glycidate, ethyl vanillin, heliotropin, indol, methyl anthranilate, vanillin, amyl salicylate, coumarin, ambrox, bacdanol, benzyl salicylate, butyl anthranilate, cetalox, ebanol, cis-3-hexenyl salicylate, lilial, gamma undecalactone, gamma dodecalactone, gamma decalactone, calone, cymal, dihydro iso jasmonate, iso eugenol, lyral, methyl beta naphthyl ketone, beta naphthol methyl ether, para hydroxyl phenyl butanone, 8-cyclohexadecen-1-one, oxocyclohexadecen-2-one/habanolide, florhydral, intreleven aldehyde, amyl cinnamic aldehyde, hexyl cinnamic aldehyde, hexyl salicylate, methyl dihydro jasmonate, sandalore, veloutone, undecavertol, exaltolide/cyclopentadecanolide, zingerone, methyl cedrylone, sandela, dimethyl benzyl carbinyl butyrate, dimethyl benzyl carbinyl isobutyrate, triethyl citrate, cashmeran, phenoxy ethyl isobutyrate, iso eugenol acetate, helional, iso E super, ionone gamma methyl, pentalide, galaxolide, phenoxy ethyl propionate, and combinations comprising one or more of the foregoing fragrances.
[030] In one embodiment, preferably said fragrance ingredient is pine oil, in the range of 0.01 – 0.05 wt%.
[031] In an embodiment, said dispersion of nano-particle suspension is a suspension of metals or oxides thereof capable of disinfecting a surface for a longer period of time. The said metal or metal oxide may be a precious metal like Au, Ag etc. or other metal like Cu, Ti. In an embodiment, said nano-particle suspension is in the range of 0.05 – 0.2%.
[032] In an embodiment, the present invention also provides a process for preparation of the disinfecting cum cleaning composition.
[033] The pH booster of the composition is in the form of an alkali metal hydroxide or alkaline earth metal hydroxide. Preferably, the pH booster is caustic soda in the range of 0.1 – 0.5% to the weight of composition
[034] The pH buffering agent of the composition is an alkali metal chloride or alkaline earth metal chloride. The silicates of the said metals may also be used as buffers with an additional advantage of cleaning and abrasive properties for better surface cleaning. Preferably said buffering agent is potassium chloride in the range of 0.1 – 0.5%.
[035] A partially ionized alkali source in the composition can act as a pH buffer and stabilizer. Suitable pH buffering components for use herein can also be selected from the group consisting of alkali metal salts of carbonates, polycarbonates, sesquicarbonates, silicates, polysilicates, boron salts, aluminates and mixtures thereof. More preferably alkali metal salts of carbonate, silicate and borate. The preferred alkali metal salts for use herein are sodium and potassium. The concentration of the pH buffering agent is in the range of 0.1 – 5 % , more preferably 0.1 to 3%, and most preferably 0.1 – 2.5 %.
[036] The present invention discloses a stable cleaning composition for a range of surfaces, like glass, veneer, plastic, granite and other non-porous surfaces. The said composition containing a hypochlorite where available chlorine concentration is not less than 0.2 % at the time of packing while other commercially available spray cleaning solutions have lower amounts of Av. Cl, and this gives the composition of the invention a longer shelf life. The composition is a ready to use disinfectant formulation with stable chlorine up to 2000 ppm. The composition of the invention was found to be stable and effective for at least 9 months after preparation. This gives a longer shelf life to the composition of the invention so that less volume of product is required to be used to give the same level of disinfection as compared to commercially available cleaning solutions. The cleaning is attributed to a combination of surfactants, ionic and non-ionic, and fragrance is added to mask chlorine odour. The use of a dual surfactant system helps to solubilize the nano-particles and keep them stable in the composition given the high pH of the composition. An active nano-particle i.e. nano-silver is added to enhance its anti-microbial properties and long lasting effectiveness. Thus, the dual action of the hypochlorite to disinfect and the nano-silver to sanitize surfaces is a significant benefit of the composition. Further, after disinfection by hypochlorite, the nanoparticles deposited on the cleaned surface serve to resist reinfection of the surface.
[037] Also, after cleaning surfaces with the composition, the surface shows a lustre due to the use of the dual surfactant system highlighting that the composition not only disinfects, but also cleans dirt and grime. The primary surfactant provides the cleaning activity while the secondary surfactant is a foam booster, as shown in Fig 1 wherein Fig.1A shows a composition lacking the secondary surfactant while Fig. 1B shows the composition of the invention comprising the secondary surfactant. The secondary surfactant also serves to solubilize a fragrance. This is needed because organic fragrances are ordinarily insoluble in disinfectant formulations, and hence cause the formulations to appear hazy as shown in the Fig. 2 wherein Fig. 2A shows a composition lacking the secondary surfactant while Fig. 2B shows the composition of the invention comprising the secondary surfactant. The product is already diluted with water and hence does not need to be further diluted at the time of use. The product is packed in a spray bottle and used directly onto surfaces. Since the composition is an aqueous formulation, it needs no aerosol, pressurized container, or propellants for spraying.

[038] The following experimental examples are illustrative of the invention but not limitative of the scope thereof:
Example 1:
[039] The spray disinfectant composition of the invention was prepared by mixing 3% by weight sodium hypochlorite with 0.21% by weight caustic soda, 0.35% by weight potassium chloride, 0.2% by weight lauryl amine oxide, 0.1% by weight sodium lauryl sarcosinate, 0.05% by weight 32% pine oil, 0.1% by weight nano-silver solution in water Q.S. The composition thus prepared was tested in subsequent examples.
Example 2:
[040] The following experimental conditions were used to assess various parameters for the composition prepared in Example 1.
1. Experimental conditions:
1.1 Organisms Used: Escherichia coli ATCC 10536
Staphylococcus aureus ATCC 6538
Enterococcus hirae ATCC 10541
Pseudomonas aeruginosa ATCC 15442
1.2 Test Temperature: 24oC ± 1oC
1.3 Concentration of the Sample: Neat, 0.5%, 50%
1.4 Contact Time: 1min ± 5s, 5mins ± 10s
1.5 Interfering substance: Clean Condition (BSA)
1.6 Replicates: n=1
1.7 Neutralizer: In-house neutralizer 3
2. Culture media and reagents:
2.1 Culture medium: Tryptone Soya Agar (TSA)
2.2 Dilution fluid used: Tryptone sodium chloride (TSC) solution
2.3 Preparation Hard Water:
Preparation of stocks solutions: Solution A: 19.84g of Magnesium chloride & 46.24g of Calcium chloride was dissolved in 1000ml
glass distilled water. Solution B: 35.02g of Sodium bicarbonate was dissolved in 1000ml glass distilled water. Both the solutions were filter sterilized.
Preparation of freshly prepared hard water: 6ml solution A was added to 1000ml volumetric flask containing approximately 500-600ml glass distilled water, followed by addition of 8ml of Solution B. After adequate mixing, the volume was made up to 1000 ml with glass distilled water. pH of the freshly prepared solution was adjusted to 7.00 (±0.2) using 1N Hydrochloric acid or 1N Sodium hydroxide and was filter sterilized before use.
2.4 Interfering substance: Bovine Albumin Solution was prepared using Bovine serum albumin fraction-V (BSA): For Dirty conditions, 0.6g of BSA was dissolved in 100ml glass distilled water and filter sterilized while for clean conditions, 0.06g of BSA was dissolved in 100ml glass distilled water and filter sterilized using membrane filter.
3 Preparation of test organism suspension:
3.1 Working culture of test organisms: For first subculture, test microorganism was inoculated from glycerol stock on a sterile TSA plate and incubated at 37 ºC ±1ºC for approximately 24hrs- 48 hrs. For second subculture, growth obtained in first subculture plate was streaked on a sterile TSA plate & incubated at 37ºC ±1ºC for approximately 24hrs.
3.2 Test suspension: From second subculture, a loopful of test culture was suspended in sterile diluent (TSC) & dislodged using mechanical vortex. The optical density of test culture suspension was adjusted at 620 nm such that the initial bacterial test suspension contained 1.5 x 108 to 5 x 108 cfu/ml for E.coli, S.aureus and E.hirae. For Pseudomonas aeruginosa the test suspension contained 1.5 x 108 to 5 x 108 cfu/ml under dirty condition and 1.5 x 109 to 5 x 109 cfu/ml under clean condition. Cell strength of the suspensions was confirmed by carrying out tenfold serial dilution and plating 1 ml of the appropriate dilutions in duplicates using TSA by pour plate technique. After solidification, plates were incubated at 37 ºC ± 1ºC for 48 hrs. The test suspension was used within 2 hours of preparation.
4. Preparation of sample:
Table 1

5 Test surface preparation:
a) Prior to use, stainless steel discs were placed in (2cm diameter discs) in a beaker containing 20 ml of 5% Triton X-100 for 60mins.
b) After 60 mins, discs were rinsed under running sterile glass distilled water for 10sec.
c) The discs were again rinsed under sterile glass distilled water for 10secs to ensure complete removal of surfactant.
d) For sterilization, discs were placed in a beaker containing 70% (v/v) isopropanol for 15mins.
e) After 15 mins, discs were removed and air dried under laminar air flow for 10 mins.
f) Completely dried steel discs were used for test and validation purpose.
g) Microbial Test suspension: 1ml of the test suspension was added to tube containing to 1ml of the interfering substance, mixed and kept for 2 mins ± 10s at specified test temperature.
Immediately before addition, the microbial test suspension was mixed well to fully re-suspend the organisms.
h) In a sterile petri plate, sterile discs were placed and 0.05ml (50µl) of microbial test suspension was added and dried until they were visibly dry not exceeding 60 minute.
6. Evaluation of Antibacterial efficacy:
6.1 Neutralizer Control “NC”- verification of the absence of toxicity of the neutralizer:
a) 0.1ml of hard water was added to the sterile container containing 10 ml of neutralizer and sufficient glass beads. Mix & kept for 5 min + 10s.
b) At the end of neutralization time, the inoculated test surface was transferred into the container.
c) The inoculated surface was placed downwards in contact with the beads and container was shaken vigorously for 1 min.
d) 1ml of above neutralized mixture was serially 10-fold diluted up to 10-6 (up to 10-7 for Pseudomonas aeruginosa under clean conditions) and 1ml of appropriate dilutions were plated in duplicates using TSA by pour plate technique.
e) After solidification, plates were incubated at 37 ºC ± 1ºC for 48 hours.
6.2 Method Validation “NT”-dilution-neutralization validation:
a) 0.1ml of highest product concentration used in test was added into the sterile container containing 10 ml of neutralizer and sufficient glass beads. Mix & kept for 5 min + 10s.
b) At the end of neutralization time, the inoculated test surface was transferred into the container.
c) The inoculated surface was placed downwards in contact with the beads and container was shaken vigorously for 1 min.
d) 1ml of above neutralized mixture was serially 10-fold diluted up to 10-6 (up to 10-7 for Pseudomonas aeruginosa under clean conditions) and 1ml of appropriate dilutions were plated in duplicates using TSA by pour plate technique.
e) After solidification, plates were incubated at 37 ºC ± 1ºC for 48 hours.
6.3 Determination of Water Control (“Nc”):
a) 0.1ml of sterile hard water was added on to dried inoculated test surface ensuring that the dried inoculum is totally covered for specified contact time (Point 1.4).
b) At the end of contact time, the disc was transferred to a container containing 10ml of neutralizer together with sufficient glass beads to support the surface.
c) The surface was placed with the inoculated surface downwards in contact with the beads and container was shaken vigorously for 1 min.
d) After the neutralization time of 5 mins ± 10s, this mixture was serially 10-fold diluted up to 10-6 (up to 10-7 for Pseudomonas aeruginosa under clean conditions) and 1ml of appropriate dilutions were plated in duplicates using TSA by pour plate technique.
e) After dilution, the test surface (Nts) was recovered by draining off the neutralizer. It was rinsed with 10 ml of glass distilled water and then placed on a petri-dish containing solidified TSA ensuring that the test side faces upward. The test surface was covered by pouring TSA on it.
f) After solidification, plates were incubated at 37 ºC ± 1ºC for 48 hours.
6.4 Determination of Microbicidal Concentrations (“Nd”):
a) 0.1ml of test sample was added on to dried inoculated test surface ensuring that the dried inoculum is totally covered for specified contact time (Point 1.4).
b) At the end of contact time, the disc was transferred to a container containing 10ml of neutralizer together with sufficient glass beads to support the surface.
c) The surface was placed with the inoculated surface downwards in contact with the beads and container was shaken vigorously for 1 min.
d) After the neutralization time of 5 mins + 10s, this mixture was serially 10-fold diluted up to 10-5. 1ml from neutralized mixture and each dilution was plated in duplicates using TSA by pour plate technique.
e) After dilution, the test surface (Nts) was recovered by draining off the neutralizer. It was rinsed with 10 ml of glass distilled water and then placed on a petri-dish containing solidified TSA ensuring that the test side faces upward. The test surface was covered by pouring TSA on it.
f) After solidification, plates were incubated at 37oC ± 1ºC for 48 hours.
6.5 Counting and calculations of the test mixture and validation mixture:
Post incubation, plates having colonies in range of 14 to 330 were counted and considered for calculation. If one or both of the duplicate values for Nd are either below the lower or above the upper limit, results are expressed as “less than” or “more than”. In particular, in the assay, where the number of cfu on every plate counted is < 14, the number of cfu/ml should be recorded as <1.40 x 102 (<2.15 Log10).Where the number of cfu on every plate counted >330 number of cfu/ml should be recorded as > 3.3 x 105 (>5.52 Log10).
If both of the duplicate values for Nd are zero, <0.10 Log10 would be considered.
Numbers of cfu/ml in the test suspension “N” along with Numbers of cfu/ml in the test mixture “Nd” and in the validation mixtures NC, NT was using the following formulae:
N = Log10 0.025 x c/(n1+0.1n2) d
Where, c = sum of all counted colonies taken into account
d= dilution factor corresponding to the lower dilution
n1 = number of plates in lower dilution counted
n2 = number of plates in higher dilution counted
Nd, Nc, NC, NT= log(c x 10/ n x d)
Where, c = sum of all counted colonies taken into account
n = number of counted plates taken into account as described for N
d = dilution taken into account
6.6 Expression of results:
a) The test results were expressed as log reduction values. For each test organism, the number of cfu/ml in the water control “Nc” and the test mixture “Nd” was calculated. For each test sample concentration and each experimental condition, the decimal log reduction value was calculated and recorded.
Reduction in viability= R (Log10 Reduction) = Log10 Nc – Log10 Nd
Where, Nc - Number of cells per ml in the Water Control
Nd- Number of survivor per ml in the Test Mixture
[041] Table 2 shows results of bactericidal efficacy testing of the composition of Example 1:
Table 2

Example 3:
[042] Next, microbicidal efficacy of the composition on fungi was assessed using experimental conditions similar to Example 2, unless stated otherwise below:
1 Test micro-organism Candida albicans ATCC 10231 and Aspergillus brasilensis ATCC 16404.
2 Culture medium used was Malt Extract Agar (MEA) comprising Malt Extract 30.0g, Agar 15.0g and Water 1000ml.
3 Preparation of test organism suspension:
3.1 Working culture of test organisms:
a) Candida albicans (yeast): For first subculture, test microorganism was inoculated from glycerol stock on a sterile MEA plate and incubated at 30oC ±1ºC for approximately 48hrs. For second subculture, growth obtained in first subculture plate was streaked on a sterile MEA plate and incubated at 30oC ±1ºC for approximately 48hrs.
b) Aspergillus brasiliensis (mould): Test microorganism was inoculated from glycerol stock on a sterile MEA plate and incubated at 30oC ±1ºC for approximately 7-9days
3.2 Test suspension:
a) Candida albicans (yeast): From second subculture, a loopful of test culture was suspended in sterile diluent (TSC) and dislodged using mechanical vortex. The optical density of test culture suspension was adjusted at 620 nm such that the initial bacterial test suspension contained 1.5 x 107 to 5 x 107cfu/ml under dirty condition and 1.5 x 108 to 5 x 108 cfu/ml under clean condition. Cell strength of the suspensions was confirmed by carrying out tenfold serial dilution and plating 1 ml of the appropriate dilutions in duplicates using MEA by pour plate technique. After solidification, plates were incubated at 30ºC ± 1ºC for 48 hrs. The test suspension was used within 2 hour of preparation.
b) Aspergillus brasiliensis (mould): Sterile water containing polysorbate 80 (Tween 80) (0.05%) was added onto the growth obtained from subculture and using sterile loop, the spores were gently scraped from the surface. The spore suspension was filtered through glass wool and then the strength was adjusted to 1.5 x107 to 5.0 x107 cfu/ml. The prepared spore suspension was enumerated by haemocytometer device and by viability count method wherein successive tenfold dilution of the suspension was carried out to confirm the spore strength. Cell strength of the suspensions was confirmed by carrying out tenfold serial dilution and plating 1 ml of the
appropriate dilutions in duplicates using MEA by pour plate technique. After solidification, plates were incubated at 30oC ± 1ºC for 48 hrs.
[043] Preparation of sample and test surface preparation were done as per Example 2. Similarly, NC, NT and Nc were prepared according to Example 2, except that for Nd, 1ml of the neutralized mixture was serially 10-fold diluted up to 10-5 (up to 10-6 for Candida albicans under clean conditions) and for Nd, serial dilution was up to 10-4 and for all, after solidification, plates were incubated at 30oC ± 1oC for 48 hours.
[044] Table 3 is a summary of the results of the microbicidal efficacy testing of the composition of the invention:
Table 3

Example 4
[045] Next, microbicidal efficacy of the composition on viruses was assessed using experimental conditions similar to Example 2, unless stated otherwise below:
1 Test micro-organism
MS2 Bacteriophage ATCC 15597 - B1, Bacterial Host – Escherichia coli ATCC 15597
Concentration of the Sample: 0.5%, 0.75%, 1%, 2%, 50%, Neat
Culture medium: Luria Agar (LA)
Preparation of test organism suspension: MS2 Bacteriophage: Bacteriophage was propagated using E. coli host culture to achieve virus particle strength of 1.5 x 108 to 5 x 108 pfu/ml. Particle strength of the suspensions was confirmed by carrying out tenfold serial dilution and plating 1 ml of the appropriate dilutions in duplicates using overlay method. After solidification, plates were incubated at 37oC ± 1ºC for 24 hrs.
Preparation of sample:
Table 4

[046] Preparation of sample and test surface preparation were done as per Example 2. Similarly, NC, NT and Nc were prepared according to Example 2, except that for Nd, 1ml of the neutralized mixture was serially 10-fold diluted up to 10-5 and 1ml of 10-3, 10-4, 10-5 dilutions were plated in duplicates using overlay method. For all, after solidification, plates were incubated at 37ºC ± 1ºC for 24 hrs.
[047] Table 5 is a summary of the results of the microbicidal efficacy testing of the composition.
Table 5

[048] The above examples are non-limiting. The invention is defined by the claims that follow:
,CLAIMS:1. A cleaning disinfectant composition for spraying on surfaces, said composition comprising:
- a hypochlorite of an alkali metal or a hypochlorite of an alkaline earth metal in a range of 0.25 to 5% by weight,
- a primary surfactant in a range of 0.1 to 0.5% by weight,
- a secondary surfactant in a range of 0.1 to 0.5% by weight,
- a nanoparticle suspension in a range of 0.05 to 0.2% by weight,
- a pH booster in a range of 0.1 to 0.5% by weight, and
- a pH buffering agent in a range of 0.1 to 0.5% by weight,
of the total composition in water, wherein,
the primary surfactant is an anionic organic surfactant or a cationic surfactant having cleaning ability, and the secondary surfactant is a non-ionic or amphoteric organic surfactant having foam boosting ability,
wherein the composition optionally comprising a fragrance in a range of 0.01 to 0.05% by weight of the total composition.

2. The composition as claimed in claim 1, wherein the hypochlorite is selected from sodium hypochlorite, calcium hypochlorite, lithium hypochlorite or a combination thereof.

3. The composition as claimed in claim 1, wherein the fragrance is selected from the group consisting of anethol, methyl heptine carbonate, ethyl aceto acetate, para cymene, nerol, decyl aldehyde, para cresol, methyl phenyl carbinyl acetate, undecylenic aldehyde, undecyl aldehyde, 2,6-nonadienal, nonyl aldehyde, octyl aldehyde, phenyl acetaldehyde, anisic aldehyde, benzyl acetone, ethyl-2-methyl butyrate, damascenone, damascone alpha, damascone beta, flor acetate, frutene, fructone, herbavert, iso cyclo citral, methyl isobutenyl tetrahydro pyran, isopropyl quinoline, 2,6-nonadien-1-ol, 2-methoxy-3-(2-methylpropyl)-pyrazine, methyl octine carbonate, tridecene-2-nitrile, allyl amyl glycolate, cyclogalbanate, cyclal C, melonal, gamma nonalactone, cis 1,3-oxathiane-2-methyl-4-propyl, benzaldehyde, benzyl acetate, camphor, camphene, carvone, borneol, bornyl acetate, decyl alcohol, eucalyptol, linalool, hexyl acetate, iso-amyl acetate, thymol, carvacrol, limonene, menthol, iso-amyl alcohol, phenyl ethyl alcohol, alpha pinene, a terpineol, citronellol, alpha thujone, benzyl alcohol, beta gamma hexenol, dimethyl benzyl carbinol, phenyl ethyl dimethyl carbinol, adoxal, allyl cyclohexane propionate, beta pinene, citral, citronellyl acetate, citronellal nitrile, dihydro myrcenol, geraniol, geranyl acetate, geranyl nitrile, hydroquinone dimethyl ether, hydroxycitronellal, linalyl acetate, phenyl acetaldehyde dimethyl acetal, phenyl propyl alcohol, prenyl acetate, triplal, tetrahydrolinalool, verdox, cis-3-hexenyl acetate, ethyl methyl phenyl glycidate, ethyl vanillin, heliotropin, indol, methyl anthranilate, vanillin, amyl salicylate, coumarin, ambrox, bacdanol, benzyl salicylate, butyl anthranilate, cetalox, ebanol, cis-3-hexenyl salicylate, lilial, gamma undecalactone, gamma dodecalactone, gamma decalactone, calone, cymal, dihydro iso jasmonate, iso eugenol, lyral, methyl beta naphthyl ketone, beta naphthol methyl ether, para hydroxyl phenyl butanone, 8-cyclohexadecen-1-one, oxocyclohexadecen-2-one/habanolide, florhydral, intreleven aldehyde, amyl cinnamic aldehyde, hexyl cinnamic aldehyde, hexyl salicylate, methyl dihydro jasmonate, sandalore, veloutone, undecavertol, exaltolide/cyclopentadecanolide, zingerone, methyl cedrylone, sandela, dimethyl benzyl carbinyl butyrate, dimethyl benzyl carbinyl isobutyrate, triethyl citrate, cashmeran, phenoxy ethyl isobutyrate, iso eugenol acetate, helional, iso E super, ionone gamma methyl, pentalide, galaxolide, phenoxy ethyl propionate, and combinations thereof.

4. The composition as claimed in claim 1, wherein the primary surfactant is an anionic surfactant selected from the group consisting of linear and/or branched chain alkylbenzene sulfonates, alkyl sulfates, ether sulfates, secondary alkyl sulfates, a-olefin sulfonates, phosphate esters, sulfosuccinates, isethionates, carboxylates, sodium lauryl sulfate, sodium lauryl ether sulfate, triethanolamine lauryl sulfate, magnesium lauryl sulfate, sulfosuccinate esters, ammonium lauryl sulfate, alkyl sulfonates, sodium lauryl sulfate, sodium alpha olefin sulfonates, alkyl sulfates, sulfated alcohol ethoxylates, sulfated alkyl phenol ethoxylates, sodium xylene sulfonate, alkylbenzene sulfonates such as triethanolamine dodecylbenzene sulfonate, sodium dodecylbenzene sulfonate, calcium dodecylbenzene sulfonate, xylene sulfonic acid, dodecylbenzene sulfonic acid, N-alkoyl sarcosinates such as sodium lauroyl sarcosinate, dialkylsulfosuccinates, N-alkoyl sarcosines such as lauroyl sarcosine, alkyl ether carboxylates, soaps including sodium, potassium, magnesium, calcium, alkanolamine, amine soaps, and combinations thereof.

5. The composition as claimed in claim 1, wherein the primary surfactant is a cationic surfactant selected from the group comprising quaternary ammonium compounds including alkyl dimethyl benzyl ammonium chloride, dialkyl dimethyl ammonium chloride, alkyl trimethyl ammonium chloride or bromide, salts of organic or inorganic acids with fatty amines, fatty amine ethoxylates, and combinations thereof.

6. The composition as claimed in claim 1, wherein the secondary surfactant is selected from the group consisting of ethoxylated alcohols, ethoxylated phenols, ethoxylated fatty acid esters, ethoxylated glycerol fatty acid esters, sorbitan fatty acid esters, glycerol fatty acid esters, coconut monoethanolamide ethoxylates, ethoxylated tall oil, ethoxylated polypropylene glycol, fatty acid alkanolamides such as coconut mono- and diethanolamide, amine oxides such as lauryl amine oxide, myrsityl amine oxide, amido-amine oxides like lauryl di-methyl amidoamine oxide, alkylamidopropylamine N-oxide, n-alkyl pyrrolidones, alkyl polysaccharides such as sucrose esters and alkyl polyglycosides, alkyl phenol ethoxylates, ethoxylated castor oil, fatty acid ethoxylates, fatty amine ethoxylates, polyglycerol fatty acid esters, and alkyl adducts of ethylene-oxide-propylene oxide copolymers, betaines such as cocamido propyl betaine etc, and combinations thereof.

7. The composition as claimed in claim 1, wherein the primary surfactant is sodium lauryl sarcosinate and the secondary surfactant is lauryl amine oxide.

8. The composition as claimed in claim 1, wherein the nanoparticle suspension is a suspension of metals or oxides thereof.

9. The composition as claimed in claim 1, wherein the pH booster is an alkali metal hydroxide, an alkaline earth metal hydroxide or a combination thereof.

10. The composition as claimed in claim 1, wherein the pH buffering agent is selected from the group consisting of alkali metal chlorides or alkaline earth metal chlorides, alkali metal salts of carbonates, polycarbonates, sesquicarbonates, silicates, polysilicates, boron salts, aluminates and combinations thereof.