CLIAMS:1. A composition for enhanced microbial vitamin B12 production, said composition comprising:
a. fructo-oligosaccarides (FOS);
b. inulin; and
c. lactulose.
2. A method of enhancing microbial production of vitamin B12, said method comprising:
a. obtaining a vitamin B12 producing microbe; and
b. contacting (a) with a composition comprising:
i. fructo-oligosaccaride;
ii. inulin; and
iii. lactulose,
wherein, said method enhances vitamin B12 production.
3. A composition for enhanced probiotic bacterial growth, said composition comprising:
a. fructo-oligosaccarides (FOS);
b. inulin; and
c. lactulose.
4. A method of enhancing probiotic bacterial growth, said method comprising:
a. obtaining a probiotic microbe; and
b. contacting (a) with a composition comprising:
i. fructo-oligosaccaride;
ii. inulin; and
iii. lactulose,
wherein, said method enhances probiotic microbe growth.
5. A composition for enhanced short chain fatty acids production by bacteria, said composition comprising:
a. fructo-oligosaccarides (FOS);
b. inulin; and
c. lactulose.
6. The composition as claimed in claim 1 or 3 or 5, wherein the concentration of fructo-oligosaccarides or inulin or lactulose is in the range of 0.09mg/ml-0.99mg/ml.
7. The composition as claimed in claim 1, or 3, or 5, wherein the w/w ratio of fructo-oligosaccarides:inulin:lactulose is 1:1:1 or 2:4:1 or 2:2:1 or 4:2:1.
8. A method of enhancing short chain fatty acids production by bacteria, said method comprising:
a. obtaining a microbe; and
b. contacting (a) with a composition comprising:
i. fructo-oligosaccaride;
ii. inulin; and
iii. lactulose,
wherein, said method enhances short chain fatty acid production.
9. The method as claimed in claim 2, or 4 or 8, wherein the bacteria is selected from the group consisting of: Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus helveticus, L. coryniformis, L. crispatus, L. delbrueckii subsp. bulgaricus, L. delbrueckii subsp. delbrueckii, L. dellbrueckii subsp. Lactis, L. gasseri, L. jensenii, L. johnsonii, L. paraplantarum, L. reuteri, L. sakei, L. salivarius, Bacillus subtilis, Streptococcus thermophilus, Streptococcus salivarius, Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium infantis, and Lactococcus lactis, preferably Lactobacillus casei.
10. The method as claimed in claim 2, or 4 or 8, wherein the bacteria is The method as claimed in claim 2, or 4, or 8, wherein the concentration of fructo-oligosaccarides or inulin or lactulose is in the range of 0.09mg/ml-0.99mg/ml.
11. The method as claimed in claim 2, or 4, or 8, wherein the w/w ratio of fructo-oligosaccarides:inulin:lactulose is 1:1:1 or 2:4:1 or 2:2:1 or 4:2:1.
12. A formulation comprising:
a. food product;
b. probiotic;
c. fructo-oligosaccaride (FOS);
d. inulin; and
e. lactulose,
wherein said formulation has enhanced vitamin B12 content.
13. The formulation as claimed in claim 12, wherein the food product is yogurt/Dahi.
14. The formulation as claimed in claim 12, wherein the probiotic is selected from the group consisting of: Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus helveticus, L. coryniformis, L. crispatus, L. delbrueckii subsp. bulgaricus, L. delbrueckii subsp. delbrueckii, L. dellbrueckii subsp. Lactis, L. gasseri, L. jensenii, L. johnsonii, L. paraplantarum, L. reuteri, L. sakei, and L. salivarius, preferably Lactobacillus casei.
15. The formulation as claimed in claim 12, wherein the concentration of fructo-oligosaccarides or inulin or lactulose is in the range of 0.09mg/ml-0.99mg/ml.
16. The formulation as claimed in claim 12, wherein the ratio of fructo-oligosaccarides:inulin:lactulose is 1:1:1.
17. The formulation as claimed in claim 12, wherein the w/v ratio of food product: a mixture of fructo-oligosaccarides, inulin, and lactulose is in the range of 1% to 10 %, preferably 1.5%.
18. A food supplement comprising the composition as claimed in claim 1, or 3, or 5.
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