CLIAMS:1. A sunscreen composition comprising 0.001 to 5 % by weight avobenzone and 0.001 to 5 % by weight Leucas plant extract, wherein the Avobenzone and the Leucas plant extract are in a weight ratio from 1:1 to 1:100.
2. A sunscreen composition comprising 0.001 to 5 % by weight avobenzone and 0.001 to 5 % by weight Leucas plant extract, wherein the Avobenzone and the Leucas plant extract are in a weight ratio from 1:5 to 1:100.
3. The sunscreen composition as claimed in any of claims 1 or 2, wherein said composition preferably comprises 1 to 5 % by weight avobenzone and preferably 1 to 5 % by weight Leucas plant extract.
4. The sunscreen product composition as claimed in any of the preceding claims, wherein said composition is photo stable.
5. The sunscreen product composition as claimed in any of the preceding claims, wherein said composition provides the broad spectrum protection against UVC, UVB and UVA radiation.
6. The sunscreen product composition as claimed in claim 2, wherein said composition has improved protection against UV rays.
7. The sunscreen product composition as claimed in any of the preceding claims, wherein said composition further comprises cosmetically acceptable excipients selected from silicones, vitamin and/or derivatives thereof, polyols, vehicles, structurants, emollient/s, gelling agent/s, a thickening agent/s, a hydrophilic or hydrophobic polymer, an emulsifying agent/s, alcohol/s and mixtures thereof.
8. The sunscreen product composition as claimed in any of the preceding claims, wherein said composition is prepared in the form of lotions, serums, oils, sprays, gels and gel-creams.
,TagSPECI:Field of the invention
The present invention relates to a sunscreen compositions such as lotions or creams applied to the surface of human skin to prevent ultraviolet light and short wave visible light induced damage. More particularly, relates to the sunscreen composition containing Avobenzone and Leucas extract.

Background and the Prior Art
Use of sunscreen products is essential to ensure protection from the ultraviolet (UV) light of the sun, which can be divided into UVC (100–290 nm), UVB (290–320 nm), UVA I (320–340 nm), and UVA II (340–400 nm) regions. UVC rays are filtered by the atmosphere and therefore do not reach the earth’s surface. However, exposure to UVB light, which although only penetrates the upper layers of the skin, can cause DNA damage and sunburn, while the penetration of UVA light to the deeper layers of the skin causes photo-aging and DNA damage, due to the generation of reactive oxygen species (Kockler J, et al., Journal of Photochemistry and Photobiology C: Photochemistry Reviews 13: 91– 110(2012)).
The broad spectrum sunscreen products containing UV filters are thus required which offer protection from both UVB and UVA rays of the sun so that it can protect the skin from sunburn as well as aging. To combat the harmful effects of UV radiation 23 sunscreens filters comprising of 8UVA and 15 UVB filters are approved by EU and are available in the market. The Butyl methoxydibenzoylmethane or more commonly known as Avobenzone is a UVA filter approved by EU, owing to its absorption capabilities in both UVAI and UVAII regions with ?max of 357nm, combined with high specific extinction coefficient of 1100 and its compatibility with most of the organic UVB sunscreen filters. It is most prevalently used in commercial preparations which are marketed as ‘broad spectrum’ sunscreens.
Avobenzone is highly sensitive to both light and heat. The active enol form of Avobenzone absorbs UVA radiation and converts to singlet diketo form which has absorption maxima in UVC range. This singlet diketo form produces triplet diketo form which further reacts with Oxygen to form singlet state Oxygen. The triplet diketo form further produces photo-degradation products. The singlet Oxygen also reacts with enol form and produces photo-degradation products (KocklerJ,etal., Profiles of Drug Substances, Excipients, and Related Methodology, 38:87-111(2013)).
Data presented to the Food and Drug Administration by the Cosmetic, Toiletry and Fragrance Association indicates a -36% change in Avobenzone’s UV absorbance following one hour of exposure to sunlight. Apart from rapid photo-degradation of Avobenzone the transient triplet diketo form interacts with key building blocks of biomolecules by triplet–triplet energy transfer (in the case of thymidine) or electron transfer processes (for 2’-deoxyguanosine, tryptophan and tyrosine) thereby degrading them (See Cecilia Paris et al., Photochemistry and Photobiology, 85: 178–184(2009)).
Lhiaubet-Vallet et al, explored several strategies to improve photo-stability of Avobenzone in the sunscreen compositions, one of them being triplet state quenchers as exemplified by Avobenzone–Octocrylene association. Octocrylene is one of the most effective stabilizing agents of Avobenzone, but this organic compound can penetrate into the skin where it acts as a photosensitizer. It results in increased production of free radicals under illumination. Organic sunscreens such as Tinosorb M, Tinosorb S, Mexoryls or other chemicals are also effective in photo-stabilizing Avobenzone, but are expensive to be incorporated in the amounts required to photo-stabilize Avobenzone.
US20090232754 discloses enhanced photo-stability of avobezone in a sunscreen composition by menthyl anthranilate. However chemicals such as menthyl anthranilate have been found to be skin irritants.
Accordingly there is a need to provide a photo-stable composition of avobezone which does not contain chemical stabilizer which causes skin irritation. One of the research (www.cansa.org.za/sunscreen-research/ ) discloses use of plant polyphenol as potential stabilizer of photo-unstable sunscreen absorbers including Avobenzone. However there is no composition known where the photo stabilizer not only stabilises Avobenzone but also synergistically increases the UV protection ability of the composition.

Object of the present invention
It is an object of present invention to overcome the drawbacks of the prior art.
It is another object of present invention to improve the photo-stability of Avobenzone using Leucas extract in the sunscreen products.
It is yet another object of the present invention to develop a photo-stable sunscreen product using Avobenzone and Leucas extract.
It is a further object of the present invention to provide broad spectrum UVC, UVB and UVA protection with the said Avobenzone and Leucas extract sun screen product.

Summary of the invention
According to one aspect of the present invention there is provided a sunscreen composition comprising 0.001 to 5 % by weight avobenzone and 0.001 to 5 % by weight Leucas plant extract, wherein the Avobenzone and the Leucas plant extract are in a weight ratio from 1:1 to 1:100.
According to another aspect of the present invention there is provided a sunscreen composition comprising 0.001 to 5 % by weight avobenzone and 0.001 to 5 % by weight Leucas plant extract, wherein the Avobenzone and the Leucas plant extract are in a weight ratio from 1:5 to 1:100.

Brief Description of Accompanying Drawings
Fig.1 depicts the absorbance spectrum of 1part of Avobenzone, 100parts of Leucas individually and collectively in a ratio of 1:100.
Fig.1b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:100 where synergy between the molecules is observed in the means of absorption of UVA radiation from 386 to 420nm.
Fig.2 depicts the absorbance spectrum of 1part of Avobenzone, 90parts of Leucas individually and collectively in a ratio of 1:90.
Fig.2b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:90 where synergy between the molecules is observed in the means of absorption of partial UVC, whole of UVB and whole of UVA radiation from 260 to 420nm.
Fig.3 depicts the absorbance spectrum of 1part of Avobenzone, 80parts of Leucas individually and collectively in a ratio of 1:80.
Fig.3b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:80 where synergy between the molecules is observed in the means of absorption of UVA radiation from 357 to 420nm.
Fig.4 depicts the absorbance spectrum of 1part of Avobenzone, 70parts of Leucas individually and collectively in a ratio of 1:70.
Fig.4b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:70 where synergy between the molecules is observed in the means of absorption of UVA radiation from 355 to 420nm.
Fig.5 depicts the absorbance spectrum of 1part of Avobenzone, 60parts of Leucas individually and collectively in a ratio of 1:60.
Fig.5b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:60 where synergy between the molecules is observed in the means of absorption of UVA radiation from 347 to 420nm.
Fig.6 depicts the absorbance spectrum of 1part of Avobenzone, 50parts of Leucas individually and collectively in a ratio of 1:50.
Fig.6b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:50 where synergy between the molecules is observed in the means of absorption of partial UVC, whole of UVB and whole of UVA radiation from 260 to 420nm.
Fig.7 depicts the absorbance spectrum of 1part of Avobenzone, 40parts of Leucas individually and collectively in a ratio of 1:40.
Fig.7b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:40 where synergy between the molecules is observed in the means of absorption of UVA radiation from 354 to 420nm.
Fig.8 depicts the absorbance spectrum of 1part of Avobenzone, 30parts of Leucas individually and collectively in a ratio of 1:30.
Fig.8b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:30 where synergy between the molecules is observed in the means of absorption of UVA radiation from 361 to 420nm.
Fig.9 depicts the absorbance spectrum of 1part of Avobenzone, 20parts of Leucas individually and collectively in a ratio of 1:20.
Fig.9b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:20 where synergy between the molecules is observed in the means of absorption of UVA radiation from 359 to 420nm.
Fig.10 depicts the absorbance spectrum of 1part of Avobenzone, 10parts of Leucas individually and collectively in a ratio of 1:10.
Fig.10b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:10 where synergy between the molecules is observed in the means of absorption of UVA radiation from 372 to 420nm.
Fig.11 depicts the absorbance spectrum of 1part of Avobenzone, 5parts of Leucas individually and collectively in a ratio of 1:5.
Fig.11b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:5 where synergy between the molecules is observed in the means of absorption of UVA radiation from 329 to 381nm.
Fig.12 depicts the absorbance spectrum of 1part of Avobenzone, 1parts of Leucas individually and collectively in a ratio of 1:1.

DETAILED DESCRIPTION OF PRESENT INVENTION
The present invention provides a sunscreen composition containing Avobenzone and a plant extract which is Leucas extract that mitigates the photo degradation of Avobenzone.
The present inventors have discovered that when extract of Leucas aspera is present in a sufficient amount with Avobenzone, Avobenzone’s photo stability is increased several folds even over long exposure to ultraviolet radiation. The mitigation of Avobenzone photo-degradation is achieved to an extent even by the lowest concentration of Leucas aspera within 15min of solar exposure.
The composition thus can be formed into a sunscreen composition. The sunscreen composition comprises 0.001 to 5 %, preferably 1 to 5 % by weight avobenzone and 0.001 to 5 %, preferably 1 to 5 % by weight Leucas plant extract.
According to the present invention there is provided a sunscreen product composition comprising Avobenzone and Leucas aspera plant extract that minimizes the photo degradation of Avobenzone to 34% even after 2 hours of exposure to simulated solar radiation. In present invention photo-stabilization of Avobenzone by Leucas aspera plant extract is attained from 1:1 to 1:100 ratios. The photo-stability of Avobenzone imparted by Leucas is attained as soon as 15minutes of exposure to simulated solar radiation.
In another embodiment of present invention the sunscreen product comprising combination of Leucas extract and Avobenzone provides a broad spectrum protection against UVC, UVB and UVA radiation while being synergistic in absorbing UVA radiation.
The composition of Avobenzone and Leucas aspera plant extract in ratio from 1:5 to 1:100 shows the synergistic UVA radiation absorption.
Leucas aspera plant extract employed in the present invention has been obtained from Universal Good Life Center, Coimbatore, Tamil Nadu, India.
In a preferred embodiment, the composition comprises an emulsion, suspension, or dispersion, solutions, colloids etc. Preferable forms of the compositions include solutions, nanoemulsion, micro-emulsions, encapsulates, liposomes, micelles, gels and other conventional formulation kinds as are commonly used to deliver cosmetic benefits.
The compositions of the present invention may contain a wide range of additional, optional components which are referred to herein as "cosmetically acceptable ingredients".
The cosmetically acceptable ingredients includes the cosmetically acceptable additives as may be added to the composition of the present invention include but are not limited to silicones, vitamin and/or derivatives thereof, polyols, vehicles, structurants, emollient/s, gelling agent/s, a thickening agent/s, a hydrophilic or hydrophobic polymer, an emulsifying agent/s, alcohol/s etc. Examples of these ingredients include but are not limited to such substances as binders, emollients, preservatives (such as methyl paraben), colorants, perfumes, skin-lightening agents, anti-wrinkle agents, antimicrobials and the like.
The polyols used are selected from glycerol, ethylene glycol, propylene glycol, pentaerythritol, diglycerol, polyglycerol, their derivatives and combinations thereof. In a preferred embodiment the invention relates to compositions wherein the concentrations of polyol compounds varies from about 0.1 to 10.0 percent by weight; preferably between 0.5 and 5.0 percent by weight, most preferably between 1.0 and 4.0 percent by weight.
The silicone used in the invention is selected from linear, branched, cross linked, silicone oils, volatile and non volatile silicones such as dimethiconecopolyol, dimethylpolysiloxane, diethylpolysiloxane, high molecular weight dimethicone, mixed C1-C30 alkyl polysiloxane, phenyl dimethicone, dimethiconol, cyclopentasiloxane, dimethicone, dimethiconol, mixed C1-C30 alkyl polysiloxane, and mixtures thereof. The concentration of silicones is about 0.01 to 5.0 percent by weight; preferably about 0.1 to 3.0 percent by weight, more preferably about 0.5 to 2.0 percent by weight.
The vitamin used in the composition is selected from a group comprising vitamin A, vitamin B (1-12), vitamin C, vitamin D (2-4), vitamin E, vitamin K, their derivatives, such as acetates, propionates, palmitates, phosphates, alone on in combinations thereof. The concentrations of the vitamin and/or its derivative(s) may be about 0.01 to 5.0 percent by weight; preferably about 0.05 and 3.0 percent by weight, and more preferably between 0.5 and 2.0 percent by weight.
Emulsifying agents which may be optionally added to the compositions of the present invention include but are not restricted to oxyalkylenated fatty acid esters of polyols, for example polyethylene glycol stearates, for instance PEG-100 stearate, PEG-50 stearate and PEG-40 stearate; and mixtures thereof, mixture of glycerylmonostearate and of polyethylene glycol stearate (100 EO) (Simulsol 165), oxyalkylenated fatty acid esters of sorbitan comprising, for example, from 20 to 100 EO such as Tween 20 or Tween 60, oxyalkylenated (oxyethylenated and/or oxypropylenated) fatty alcohol ethers; alkoxylated or non-alkoxylated sugar esters, such as sucrose stearate and PEG-20 methylglucosesesquistearate; sorbitan esters such as the sorbitanpalmitate (Span 40), esters of diacid and of fatty alcohol, such as dimyristyl tartrate; mixtures of these emulsifiers, for instance the mixture of glyceryl stearate and of PEG-100 stearate (Arlacel 165), and mixtures comprising these emulsifiers, such as the mixture of dimyristyl tartrate, cetearyl alcohol, Pareth-7 and PEG-25 laureth-25, (Cosmacol PSE) , steareth – 2, steareth 21, PPG-15 stearyl ether. The concentrations of the emulsifying agents may be about 0.1 percent by weight to about 8.0 percent by weight; preferably about 0.4 percent by weight to about 4.0 percent by weight.
Thickeners which may be used in the instant invention include but are not restricted to alkyloamides, carbomer 934,940,941,960,961, cetearyl alcohol, cetyl alcohol, gelatin, gums, and magnesium aluminium silicates, ozocarite, paraffin, tragacanth, sodium alginate, Tinovis ADM, Acrylates/C10-30 Alkyl Acrylate Crosspolymer and ammonium Acryloyl - dimethyltaurate / Vinyl pyridine Copolymer/Carbomer, Hydroxyethyl Acrylate / Sodium AcryloyldimethylTaurate Copolymer (and) Isohexadecane (and) Polysorbate 60, Simugel INS 100 , CarbopolUltrez 10 and the like. The concentrations of the thickeners may be about 0.1 percent by weight to about 2 percent by weight; preferably about 0.5 percent by weight to about 1 percent by weight.
Structurants which may be used in compositions of the present invention include those materials which are well known in the art and include fatty acids, fatty alcohols, fatty acid esters, and fatty acid amides, having fatty chains of from 8 to 30 carbons atoms. Preferably the structurant used is stearic acid. The concentrations of the structurants may be about 0.1 percent by weight to about 8.0 percent by weight; preferably about 0.4 percent by weight to about 4.0 percent by weight.
Various emollients as are known to a person skilled in the art and as are available in the market can be employed as an optional constituent in the instant invention. These include but are not restricted to the group consisting of lanolin, octyldodecanol, hexyl decanol, oleyl alcohol, decyloleate, isopropyl stearate, isopropyl palmitate, isopropyl myristate, hexyl laureate, dioctyl cyclohexane, PPG-15 stearyl ether, isohexadecane, stearic acid, cetyl alcohol, mineral oil etc. The concentrations of the emollients may be about 0.1 to 10 percent by weight; preferably about 1 to 5 percent by weight.
Various chelating agents may be used as optional constituents of the instant invention. These may be selected from a group consisting of but not limited to Dimercaptosuccinic acid (DMSA), Dimercapto-propane sulfonate (DMPS), Alpha lipoic acid (ALA), Calcium disodium versante (CaNa2-EDTA), Disodium EDTA, Dimercaprol (BAL). The concentrations of the various chelating agents may be about 0.1 to 1 percent by weight; preferably about 0.2 to about 0.5 percent by weight.
Various cosmetically and dermatologically suitable preservatives may be added to the instant composition. These may be selected from the group consisting of but not restricted to 2-phenoxyethanol, para-hydroxybenzoic acid esters, also known as parabens, for instance methyl para-hydroxybenzoate (methyl paraben), ethyl para-hydroxybenzoate (ethyl paraben) and propyl para-hydroxybenzoate (propyl paraben) and mixtures thereof; formaldehyde-releasing agents, for instance imidazolidinylurea or diazolidinylurea; haloalkynylcarbamates, for instance 3-iodo-2-propynyl butyl carbamate (IPBC); caprylyl glycol, also known as 1,2-octanediol; sodium benzoate; N-(3-chloroallyl)-hexaminium chloride (or Quaternium-15); polyhexamethylenebiguanide hydrochloride (CTFA name: polyaminopropylbiguanide); alkyltrimethylammonium bromides, for instance dodecyltrimethylammonium bromide, myristyltrimethylammonium bromide and hexadecyltrimethylammonium bromide, and mixtures thereof. The concentrations of the various cosmetically and dermatologically suitable preservatives may be about 0.05 percent by weight to 1.5 percent by weight; preferably about 0.1 percent by weight and 1 percent by weight.
Anti-ageing active agents which may optionally added to the compositions of the instant invention may be chosen from free-radical scavengers, keratolytic agents, vitamins, anti-elastase and anti-collagenase agents, proteins, fatty acid derivatives, steroids, trace elements, bleaching agents, algal and plankton extracts, enzymes and coenzymes, flavonoids, ceramides, tensioning agents and muscle relaxants, and mixtures thereof. The concentrations of the Anti-ageing active agents may be about 0.001 to about 10 percent by weight, preferably from about 0.01 to about 5 percent by weight.
Free-radical scavengers and antioxidants which may be optionally added to the compositions of the instant invention include but are not restricted to phosphonic acid derivatives such as (methylenephosphonic acid), methylene phosphonic acid, methylenephosphonic acid and salts thereof, in particular the sodium salts thereof; ethylenediaminetetraacetic acid and its salts, such as the sodium salt; guanosine; superoxide dismutase; tocopherol (vitamin E) and its derivatives (acetate); ethoxyquine; lactoferrin; lactoperoxidase, and nitroxide derivatives; superoxide dismutases; glutathione peroxidase; plant extracts with free-radical-scavenging activity, such as the aqueous extract of wheatgerm (Detoxiline), green tea, and mixtures thereof. The concentrations of the Free-radical scavengers and antioxidants may be about 0.001 to about 2 percent by weight, preferably from about 0.01 to about 1 percent by weight.
The composition of the present invention may further include one or more neutralizers, such as, for example, strong and weak bases. Any suitable neutralizer can be selected, as will be appreciated by one of ordinary skill in the art. Exemplary neutralizers suitable for use in the compositions of the present invention included sodium hydroxide, potassium hydroxide, ammonium hydroxide, diethanolamine, triethanolamine, 2-dimethylamino-2-methyl-1-propanol (DAMP), 2-aminomethyl-1propanol (aminomethyl propanol) (AMP), and the like, or combinations thereof. The neutralizer, if present, may be provided in any amount, e.g., an amount sufficient to achieve a desired pH for the composition. In this respect, the composition preferably has a pH of from about 4-9, more preferably, from about 5-8, and still more preferably from about 5.5-7. Typically, the neutralizer may be present in an amount of from about 0.01% -10% by weight of the composition.
The composition of the invention may utilize a fragrance composition comprising a blend of essential oils and synthetic aroma compounds. The blend is often diluted with a carrier like propylene glycol, vegetable oil, or mineral oil. Some examples of synthetic aroma compound that are suitable for soap bar compositions of the present invention include, but are not limited to benzaldehyde, citral, vanillin, ethyl acetate, fructone, octyl acetate, pentylbutanoate, pentylpentanoate, methyl salicylate, isoamyl acetate, limonene, citronellol, and mixtures thereof. Preferably, the fragrance containing the essential oil is present in the composition of the invention in an amount between approximately 0.1% to approximately 2% by weight.
Various cosmetically acceptable vehicles may be used for the preparation of compositions as per the instant invention. These may be selected from the group consisting of but not restricted to water, alcohols, oils and combinations thereof.
Accordingly, when the surface contemplated is skin, the composition of the present invention may contain ingredients, which are added to known creams, lotions, ointments, gels or medicaments, which are physiologically acceptable to skin and which do not contain ingredients, which will reverse or retard the action of composition of Avobenzone and Leucas extract.
The composition of the present invention can be prepared by conventional methods wherein said lotion/cream consists of a water phase, an oil phase, and a post emulsification phase consisting of the said water and oil phase. The said water phase comprises of distilled water, chelating agent, thickening agent, and an emulsifier and said oil phase comprises of Avobenzone, and an emulsifier. The said post emulsification phase comprises of neutralizer and Leucas extract.
According to a preferred embodiment of the present invention said method comprises steps of:
a) preparing a water phase by adding and mixing ingredients i) water ii) EDTA, iii) Carbopol Ultrez 10, and iv) Brij 721, and heating said mixture to 45-85ºC;
b) preparing an oil phase by melting Brij 72 and Avobenzone up to 85ºC;
c) emulsifying said water phase and oil phase to obtain an emulsion; and
d) adding triethanolamine and Leucas extract to said emulsion to obtain said formulation.
In certain embodiments of the present invention the composition Avobenzone and Leucas aspera are intended to use in the preparation of sun protection formulations, moisturizing formulations, lip care products, hair protective products, anti-aging formulations, skin lightning formulations. Useful sunscreen composition according to the present invention can be prepared in the form of lotions, serums, oils, sprays, gels, gel-creams and others, utilizing formulation parameters known in the art.
The examples, which are intended to be purely exemplary of the invention, should therefore not be considered to limit the invention in any way. Efforts have been made to ensure accuracy with respect to numbers used, but some experimental errors and deviations should be accounted for.

EXAMPLE 1
A lotion formulation in accordance with the present invention can have the composition as mentioned in Table 1 below

Table 1 : Sun protection lotion with Avobenzone and Leucas extracts:
Lotion/Cream # Ingredients % w/w
Water Phase 1 Distilled Water QS
2 Chelating agent(E.g. EDTA) 0.1
3 Thickening agent(E.g. CarbopolUltrez 10) 1.5
4 Emulsifier A – Water Phase(E.g. Brij 721) 2
Oil Phase 5 Avobenzone 1
6 Emulsifier B – Oil Phase(E.g. Brij 72) 1
Post emulsification 7 Triethanolamine 1.5
8 Leucas extract 1

The Process for the preparation of sunscreen lotion/cream comprises the steps of:
a) Preparing water Phase by adding and mixing of ingredients 1 to 4 at predetermined temperature ranges from 45ºC to 85ºC
b) Preparing oil phase by melting ingredients and maintain the temperature to 850C.
c) Emulsifying water Phase of Step 1 with the Oil Phase of Step 2 and
d) Adding ingredients 6, 7 and 8 to the emulsion to obtain cosmetic compositions of the present invention.

Example 2
A spray formulation in accordance with the present invention can have the composition as mentioned in Table 2 below

Table 2 : Sun protection spray with Avobenzone and Leucas extracts
Lotion/Cream # Ingredients % w/w
Water` Phase 1 Ethanol QS
2 Diethyl phthalate 0.1
3 Avobenzone 1
4 Leucas extract 1

The Process for the preparation of spray comprises the steps of:
a) Adding Avobenzone to the ethanol.
b) Stirring the ethanol solution until Avobenzone gets dissolved completely.
c) Adding rest of the ingredients to the Avobenzone and ethanol solution obtained in step b).
d) Stirring said mixture and compensating the loss of ethanol

Example 3
Avobenzone's photo stability was evaluated with different compositions of Leucas aspera extract and Avobenzone that are differed only in the amount of Leucas aspera extract.
Avobenzone was obtained from Symrise, Mumbai, India.
Leucas extract was obtained from Universal Good Life Center, Coimbatore, Tamil Nadu, India.
Avobenzone and Leucas extract was mixed in various ratios as show below in Table 3 and evaluated for the effect of the composition on photo-degradation of Avobenzone by UV radiation. Avobenzone either alone or in combination with different ratios of Leucas extract was mixed in water and UV absorption spectrum of the solution was recorded using a spectrophotometer. Avobenzone alone and in combination with different ratios of Leucas was then exposed to simulated solar radiation for two hours (120min) wherein, at intervals of 15 minutes, 60 minutes, and 120 minutes the UV absorption spectrum of the solutions was recorded. Photo-degradation of Avobenzone (either alone or in combination with Leucas extract) was determined by comparing the initial absorbance value of Avobenzone at its ?max of 357nm to that of the absorbance values obtained after exposure to simulated solar radiation.
Table 3 depicts the photo-degradation of Avobenzone individually and in combination with increasing concentration of Leucas extract namely from 1:1 to 1:100 ratios. Avobenzone degrades to 52, 90 and 95% after 15min, 1 and 2hrs of exposure to simulated solar radiation respectively. This photo-degradation is surprisingly mitigated by Leucas aspera extract to 35, 73 and 83% at 15min, 1hour and 2hours in 1:1 ratio and to 20, 31 and 34% in 1:100 ratios of Avobenzone and Leucas respectively.
Table 3 depicts the photo-degradation of Avobenzone individually and in combination with increasing concentration of Leucas aspera.
Table 3
Percentage Photo-degradation of Avobenzone@357nm
Test sample details 15min UV Irradiation 60minUV Irradiation 120min UV Irradiation
10ppm Avobenzone 52 90 95
10ppm Avobenzone + 10 ppm Leucas 35 73 83
10ppm Avobenzone + 50 ppm Leucas 32 63 80
10ppm Avobenzone + 100 ppm Leucas 34 59 71
10ppm Avobenzone + 200 ppm Leucas 33 58 70
10ppm Avobenzone + 300 ppm Leucas 30 58 66
10ppm Avobenzone + 400 ppm Leucas 28 53 61
10ppm Avobenzone + 500 ppm Leucas 30 52 58
10ppm Avobenzone + 600 ppm Leucas 25 45 48
10ppm Avobenzone + 700 ppm Leucas 24 40 45
10ppm Avobenzone + 800 ppm Leucas 21 39 42
10ppm Avobenzone + 900 ppm Leucas 22 38 41
10ppm Avobenzone + 1000 ppm Leucas 20 31 34

It is observed from Table 3 that photo degradation of Avobenzone decreases several folds with increasing concentrations of Leucas at 15min, 1 and 2hours of exposure to simulated solar radiation.

Example 4
The UV absorbing ability of composition comprising Avobenzone and Leucas extract was evaluated with different compositions of Leucas aspera extract and Avobenzone that are differed only in the combining ratio of Leucas aspera extract and Avobenzone.
Avobenzone and Leucas extract was mixed in various w/w ratios ranging from 1:1-1:100, and evaluated for their effect on absorbing UV radiation of various wavelengths by the process mentioned in Example 3.
Fig.1 depicts the absorbance spectrum of 1part of Avobenzone, 100parts of Leucas individually and collectively in a ratio of 1:100. The graph of ‘Avobenzone and Leucas at 1:100’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 261 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 261 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.1b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:100 where synergy between the molecules is observed in the means of absorption of UVA radiation from 386 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:100’ and ‘Calculated additive value of Avobenzone and Leucas at 1:100’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.2 depicts the absorbance spectrum of 1part of Avobenzone, 90parts of Leucas individually and collectively in a ratio of 1:90. The graph of ‘Avobenzone and Leucas at 1:90’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 260 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 259 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.2b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:90 where synergy between the molecules is observed in the means of absorption of partial UVC, whole of UVB and whole of UVA radiation from 260 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:90’ and ‘Calculated additive value of Avobenzone and Leucas at 1:90’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.3 depicts the absorbance spectrum of 1part of Avobenzone, 80parts of Leucas individually and collectively in a ratio of 1:80. The graph of ‘Avobenzone and Leucas at 1:80’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 259 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.3b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:80 where synergy between the molecules is observed in the means of absorption of UVA radiation from 357 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:80’ and ‘Calculated additive value of Avobenzone and Leucas at 1:80’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.4 depicts the absorbance spectrum of 1part of Avobenzone, 70parts of Leucas individually and collectively in a ratio of 1:70. The graph of ‘Avobenzone and Leucas at 1:70’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 260 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.4b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:70 where synergy between the molecules is observed in the means of absorption of UVA radiation from 355 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:70’ and ‘Calculated additive value of Avobenzone and Leucas at 1:70’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.5 depicts the absorbance spectrum of 1part of Avobenzone, 60parts of Leucas individually and collectively in a ratio of 1:60. The graph of ‘Avobenzone and Leucas at 1:60’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 261 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 261 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.5b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:60 where synergy between the molecules is observed in the means of absorption of UVA radiation from 347 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:60’ and ‘Calculated additive value of Avobenzone and Leucas at 1:60’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.6 depicts the absorbance spectrum of 1part of Avobenzone, 50parts of Leucas individually and collectively in a ratio of 1:50. The graph of ‘Avobenzone and Leucas at 1:50’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 261 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.6b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:50 where synergy between the molecules is observed in the means of absorption of partial UVC, whole of UVB and whole of UVA radiation from 260 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:50’ and ‘Calculated additive value of Avobenzone and Leucas at 1:50’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.7 depicts the absorbance spectrum of 1part of Avobenzone, 40parts of Leucas individually and collectively in a ratio of 1:40. The graph of ‘Avobenzone and Leucas at 1:40’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 261 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 260 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.7b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:40 where synergy between the molecules is observed in the means of absorption of UVA radiation from 354 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:40’ and ‘Calculated additive value of Avobenzone and Leucas at 1:40’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.8 depicts the absorbance spectrum of 1part of Avobenzone, 30parts of Leucas individually and collectively in a ratio of 1:30. The graph of ‘Avobenzone and Leucas at 1:30’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 261 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 258 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.8b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:30 where synergy between the molecules is observed in the means of absorption of UVA radiation from 361 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:30’ and ‘Calculated additive value of Avobenzone and Leucas at 1:30’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.9 depicts the absorbance spectrum of 1part of Avobenzone, 20parts of Leucas individually and collectively in a ratio of 1:20. The graph of ‘Avobenzone and Leucas at 1:20’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 261 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 259 to 405nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.9b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:20 where synergy between the molecules is observed in the means of absorption of UVA radiation from 359 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:20’ and ‘Calculated additive value of Avobenzone and Leucas at 1:20’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.10 depicts the absorbance spectrum of 1part of Avobenzone, 10parts of Leucas individually and collectively in a ratio of 1:10. The graph of ‘Avobenzone and Leucas at 1:10’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 256 to 396nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.10b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:10 where synergy between the molecules is observed in the means of absorption of UVA radiation from 372 to 420nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:10’ and ‘Calculated additive value of Avobenzone and Leucas at 1:10’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.11 depicts the absorbance spectrum of 1part of Avobenzone, 5parts of Leucas individually and collectively in a ratio of 1:5. The graph of ‘Avobenzone and Leucas at 1:5’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 256 to 396nm and Avobenzone alone absorbs UVA radiation from 309-415nm.
Fig.11b depicts the synergy demonstrated by the combination of Avobenzone and Leucas in a ratio of 1:5 where synergy between the molecules is observed in the means of absorption of UVA radiation from 329 to 381nm. (The area of the graphs ‘Experimental value of Avobenzone and Leucas at 1:5’ and ‘Calculated additive value of Avobenzone and Leucas at 1:5’ where the Experimental value is higher than the calculated additive value represents the area where synergy is observed between the molecules).
Fig.12 depicts the absorbance spectrum of 1part of Avobenzone, 1parts of Leucas individually and collectively in a ratio of 1:1. The graph of ‘Avobenzone and Leucas at 1:1’ shows that this combination provides broad spectrum protection by absorbing UV radiation from 262 to 420nm encompassing partial UVC and whole of UVB and whole of UVA radiation, whereas Leucas alone absorbs partial UVC, UVB and UVA radiation from 256 to 396nm and Avobenzone alone absorbs UVA radiation from 309-415nm.