DESC:A COMPOSITION COMPRISING EUCALYPTUS EXTRACT AND USES THEREOF ,CLAIMS:1. A composition comprising eucalyptus plant extract, wherein said extract promotes hair growth.
2. A composition comprising eucalyptus plant extract, wherein said extract inhibits intracellular reactive oxygen species formation.
3. The composition as claimed in claim 1 or 2, further comprising suitable carriers, diluents, and excipients.
4. The composition as claimed in any of the claims 1-3, wherein said extract is prepared by a method selected from a method consisting of lyophilization, liquid preparation, and solvent extraction.
5. The composition as claimed in claim 4, wherein
a. said extract prepared by lyophilization method is obtained by:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. freeze drying said paste using a mixture of liquid nitrogen and acetone to obtain a frozen paste followed by lyophilization to obtain said extract, or
b. said extract prepared by liquid preparation method is obtained by:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with soy lecithin, glycerin, phenoxyethanol, xanthan gum, and deionized water to obtain said extract, wherein paste weight percentage in said extract is in the range of 7-12%, said soy lecithin weight percentage in said extract is in the range of 0.1-0.2%, said glycerin weight percentage in said extract is in the range of 0.2-0.6%, said phenoxyethanol weight percentage in said extract is in the range of 1-2%, said xanthan gum weight percentage in said extract is in the range of 0.3-0.6%, and water weight percentage is to make up to 100%; or
c. said extract prepared by solvent extraction is prepared by:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with dichloromethane under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iii. drying said filtrate to obtain said extract; or
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with methanol under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iii. drying said filtrate to obtain said extract; or
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with dichloromethane under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate, and a retentate;
iii. contacting said retentate with methanol under continuous stirring for at least 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iv. drying said filtrate to obtain said extract; or
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with methanol under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate, and a retentate;
iii. contacting said retentate with dichloromethane under continuous stirring for at least 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iv. drying said filtrate to obtain said extract.
6. The composition as claimed in any of the claims 1-5 , wherein said eucalyptus plant extract weight concentration in said composition is in the range of 0.001-0.01%.

7. A method of promoting hair growth, said method comprising:
a. obtaining a composition as claimed in any of the claims 1, and 3-6; and
b. contacting said composition with hair follicles,
wherein said method promotes hair growth.
8. A method of inhibiting intracellular reactive oxygen species formation, said method comprising:
a. obtaining a composition as claimed in any of the claims 2, and 3-6; and
b. contacting said composition with a cell,
wherein said method inhibits reactive oxygen species formation in said cell.
9. A method of preparing a composition as claimed in any of the claims 1-6.
10. A composition as claimed in any of the claims 1, and 3-6 for use preparing topical formulations in promoting hair growth.
11. A method of preparing eucalyptus callus extract, said method selected from the group consisting of:
a. lyophilization method comprising the steps of:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. freeze drying said paste using a mixture of liquid nitrogen and acetone to obtain a frozen paste followed by lyophilization to obtain said extract; OR
b. liquid preparation method comprising the steps of:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with soy lecithin, glycerin, phenoxyethanol, xanthan gum, and deionized water to obtain said extract, wherein paste weight percentage in said extract is in the range of 7-12%, said soy lecithin weight percentage in said extract is in the range of 0.1-0.2%, said glycerin weight percentage in said extract is in the range of 0.2-0.6%, said phenoxyethanol weight percentage in said extract is in the range of 1-2%, said xanthan gum weight percentage in said extract is in the range of 0.3-0.6%, and water weight percentage is to make up to 100% to obtain said extract; OR
c. solvent extraction method comprising the steps of:
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with dichloromethane under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iii. drying said filtrate to obtain said extract; OR
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with methanol under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iii. drying said filtrate to obtain said extract; OR
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with dichloromethane under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate, and a retentate;
iii. contacting said retentate with methanol under continuous stirring for at least 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and
iv. drying said filtrate to obtain said extract; OR
i. obtaining eucalyptus calli and contacting with at least one preservative, and at least one anti-oxidant to obtain a paste, wherein said at least one preservative weight percentage in said paste is in the range of 0.2-0.3%, and said at least one anti-oxidant weight percentage in said paste is in the range of 0.1-0.3%;
ii. contacting said paste with methanol under continuous stirring for 18-24 hours at ambient temperature followed by filtration to obtain a filtrate, and a retentate;
iii. contacting said retentate with dichloromethane under continuous stirring for at least 18-24 hours at ambient temperature followed by filtration to obtain a filtrate; and