FIELD OF INVENTION
[001] The present disclosure relates to the field of hair growth compositions in general andhair growth compositions from natural extracts, in particular. There is provided a composition comprising scopoletin and quercetin.
BACKGROUND OF INVENTION
[002] The hair follicle is a skin appendage which is responsible for producing hair.
The growth of hair from the follicle occurs broadly in three phases: anagen- or the
growth phase; catagen- or the end growth phase; and telogen- or the rest phase. The
duration of these phases varies greatly among individuals as it is influenced by the
shape and the nature of the hair follicle of a person.
[003] The hair follicle forms early in embryonic development through interactions
between epithelial-mesenchymal cells. It is one of the few organs which undergoes
continuous cycles of regeneration and degeneration throughout an individual's
lifetime, as it is associated with stem cells.
[004] Gradual hair loss is associated with the decreasing regeneration capacity of
the hair follicle. This is largely due to extrinsic and intrinsic factors that affect the
hair follicle stem cell population. For instance, damage by UV and pollutants are
among the major extrinsic factors affecting the follicular-stem cells, while damage by
reactive oxygen species is a major intrinsic factor affecting stem cells.
[005] Several strategies are available to enhance hair growth through follicle
maintenance and stimulation. Some of these include herbal compositions to stimulate
hair follicle cells, anti-aging serums and hair follicle replacement therapies.
[006] US6030948A discloses a composition containing Tal, Tp4, or a combination
thereof for treating alopecia on the scalp of a patient.
[007] US5753263A describes a method to deliver a composition selectively
to hair follicles using a liposomal formulation.

[008] US5877209 A describes hair protective formulations which can be
administered to a subject in a manner so as to protect hair follicles against oxidative
stress.
[009] Thus, despite the plethora of available formulations and therapies available,
the maintenance of the hair follicle to enhance and sustain hair growth still remains a
problem.
SUMMARY OF INVENTION
[0010] In an aspect of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w
ratio in said composition is in the range of 1:10-10:1.
[0011] In an embodiment of the present disclosure, there is provided a method of
preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method
comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting
scopoletin, and quercetin to obtain said composition, wherein scopoletin to quercetin
w/w ratio in said composition is in the range of 1:10-10:1.
[0012] In an embodiment of the present disclosure, there is provided a composition
for use in preparing formulations to promote hair growth, said composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w
ratio in said composition is in the range of 1:10-10:1.
[0013] In an embodiment of the present disclosure, there is provided a method of
promoting hair growth, said method comprising: (a) obtaining a composition
comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition
with hair follicles, wherein scopoletin to quercetin w/w ratio in said composition is in
the range of 1:10-10:1, and said composition promotes hair growth.
[0014] These and other features, aspects, and advantages of the present subject matter
will be better understood with reference to the following description and appended
claims. This summary is provided to introduce a selection of concepts in a simplified

form. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.
BRIEF DESCRIPTION OF ACCOMPANYING DRAWINGS
[0015] The following drawings form a part of the present specification and are
included to further illustrate aspects of the present disclosure. The disclosure may be
better understood by reference to the drawings in combination with the detailed
description of the specific embodiments presented herein.
[0016] Figure 1 shows the work-flow used for processing of hair follicles and
obtaining proteomic data, in accordance with an embodiment of the present
disclosure.
[0017] Figure 2 shows the unique proteins identified in each of the hair follicle
treatments, in accordance with an embodiment of the present disclosure.
[0018] Figure 3 shows the fold change in protein levels in each of the hair follicle
treatments, in accordance with an embodiment of the present disclosure.
[0019] Figure 4 shows the fold change in protein levels of Galectin-7 in each of the
hair follicle treatments, in accordance with an embodiment of the present disclosure.
DETAILED DESCRIPTION OF THE INVENTION
[0020] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions, and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features.

[0021] For convenience, before further description of the present disclosure, certain
terms employed in the specification, and examples are delineated here. These
definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the meanings
recognized and known to those of skill in the art, however, for convenience and
completeness, particular terms and their meanings are set forth below.
[0022] The articles "a", "an" and "the" are used to refer to one or to more than one
(i.e., to at least one) of the grammatical object of the article.
[0023] The terms "comprise" and "comprising" are used in the inclusive, open sense,
meaning that additional elements may be included. It is not intended to be construed
as "consists of only".
[0024] Throughout this specification, unless the context requires otherwise the word
"comprise", and variations such as "comprises" and "comprising", will be understood
to imply the inclusion of a stated element or step or group of element or steps but not
the exclusion of any other element or step or group of element or steps.
[0025] The term "including" is used to mean "including but not limited to".
"Including" and "including but not limited to" are used interchangeably.
[0026] Carriers are substances that serve as mechanisms to improve the delivery and
the effectiveness of drugs.
[0027] A diluent (also referred to as filler, dilutant, or thinner) is a diluting agent.
[0028] An excipient is an inactive substance that serves as the vehicle or medium for
a drug or other active substance. Excipients include colouring agents, humectants,
preservatives, emollients, and combinations thereof.
[0029] Unless defined otherwise, all technical and scientific terms used herein have
the same meaning as commonly understood by one of ordinary skill in the art to
which this disclosure belongs. Although any methods and materials similar or
equivalent to those described herein can be used in the practice or testing of the

disclosure, the preferred methods, and materials are now described. All publications
mentioned herein are incorporated herein by reference.
[0030] The emphasis on improving one's physical appearance is, and has been, one
of societies' long-time pursuits. Among both men and women, luxuriant hair growth
is considered a desirable physical attribute. The growth of hair is regulated by the
activity of the hair follicle, which in turn is controlled by various extrinsic and
intrinsic factors. These varied factors often result in reduced or the lack of hair
growth. Although several treatments are available to remedy the issue of reduced hair
growth, the plethora of options available shows that a solution is yet to be found.
Further, many of the formulations and remedies available are often toxic and may
have side-effects. Thus, there is a need to provide non-toxic, or natural alternatives
that can enhance the growth of hair.
[0031] The present disclosure provides one such solution in the form of a
composition comprising the herbal ingredients: scopoletin and quercetin.
[0032] Scopoletin is a coumarin found in the root of plants in the genus Scopolia
such as Scopolia carniolica and Scopolia japonica. It can also be found in fenugreek
and in dandelion coffee. Studies have shown that scopoletin has anti-inflammatory
and antioxidation activity in vivo and in vitro (Dou et al., Int. Immunopharmacol.
2013, 17, 1037-1043; Witaicenis et al., Phytomedicine 2014, 21, 240-246).
[0033] Quercetin (3,3',4',5,7-pentahydroxyflavone, also called quercetine, sophretin,
or meletin) is a bioflavonoid. It is a naturally occurring polyphenol, shows
antioxidant, anti-inflammatory, and antiallergic activities. Several recent clinical and
preclinical findings suggest quercetin as a promising natural treatment for
inflammatory skin diseases.
[0034] In this disclosure, the combination of scopoletin and quercetin at particular
w/w ratios has been shown to promote hair follicle- associated proteins. Thus, the
present disclosure provides a solution to the problem of reduced or the lack of hair

growth, by providing a composition of scopoletin and quercetin, which promotes hair
growth.
[0035] The present disclosure is not to be limited in scope by the specific
embodiments described herein, which are intended for the purposes of
exemplification only. Functionally-equivalent products, compositions, and methods
are clearly within the scope of the disclosure, as described herein.
[0036] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w
ratio in said composition is in the range of 1:10-10:1.
[0037] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w
ratio in said composition is in the range of 1:2- 2:1.
[0038] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w
ratio in said composition is 1:1.
[0039] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage
in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in
said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w
ratio in said composition is in the range of 1:10-10:1.
[0040] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage
in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in
said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w
ratio in said composition is in the range of 1:2- 2:1.
[0041] In an embodiment of the present disclosure, there is provided a composition
comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage
in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in

said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0042] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%)- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1. [0043] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1. [0044] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0045] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0046] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2-2:1.

[0047] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%, and scopoletin to quercetin w/w ratio in said composition is 1:1. [0048] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0049] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0050] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.
[0051] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin weight percentage in said composition is in the range of 0.0001 -0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0052] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.

[0053] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0054] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin weight percentage in said composition is in the range of 0.00034%)- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0055] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0056] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0057] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is

0.0005%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0058] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2-2:1.
[0059] In an embodiment of the present disclosure, there is provided a composition comprising: (a) scopoletin; (b) quercetin; (c) at least one carrier; (d) at least one diluent; and (e) at least one excipient, wherein scopoletin w/w percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is 1:1. [0060] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0061] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0062] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.

[0063] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1. [0064] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1. [0065] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0066] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.

[0067] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0068] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; and (c) contacting scopoletin, and quercetin to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0069] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0070] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1. [0071] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of

quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.
[0072] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1. [0073] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin w/w percentage in said composition is in the range of 0.0001 -0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1. [0074] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin w/w percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0075] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in

said composition is 0.0005%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0076] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin w/w percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0077] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein scopoletin w/w percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0078] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein said solution A is prepared in an organic solvent.
[0079] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein said solution A is prepared in an inorganic solvent.
[0080] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method

comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein said solution B is prepared in an organic solvent.
[0081] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; and (ii) quercetin, said method comprising: (a) obtaining a solution A of scopoletin; (b) obtaining a solution B of quercetin; and (c) contacting said solution A, and said solution B, wherein said solution B is prepared in an inorganic solvent.
[0082] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0083] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0084] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least

one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.
[0085] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0086] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0087] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least

one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0088] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0089] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2-2:1.
[0090] In an embodiment of the present disclosure, there is provided a method of preparing a composition comprising: (i) scopoletin; (ii) quercetin; (iii) at least one

carrier; (iv) at least one diluent; and (v) at least one excipient, said method comprising: (a) obtaining scopoletin; (b) obtaining quercetin; (c) obtaining the at least one carrier, at least one diluent, and at least one excipient; and (d) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition, wherein scopoletin weight percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%, and scopoletin to quercetin w/w ratio in said composition is 1:1. [0091] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[0092] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0093] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.
[0094] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1. [0095] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage

in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
[0096] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%), quercetin weight percentage in said composition is in the range of 0.0001-0.001%), and scopoletin to quercetin w/w ratio in said composition is 1:1.
[0097] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1. [0098] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1. [0099] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), quercetin weight percentage in said composition is in the range of 0.00034%>- 0.00068%), and scopoletin to quercetin w/w ratio in said composition is 1:1.

[00100] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%, and scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
[00101] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is in the range of 1:2-2:1.
[00102] In an embodiment of the present disclosure, there is provided a composition for use in preparing formulations to promote hair growth, said composition comprising: (a) scopoletin; and (b) quercetin, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), and scopoletin to quercetin w/w ratio in said composition is 1:1. [00103] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1, and said composition promotes hair growth. [00104] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1, and said composition promotes hair growth.

[00105] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:1, and said composition promotes hair growth.
[00106] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1, and said composition promotes hair growth. [00107] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1, and said composition promotes hair growth. [00108] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is in the range of 0.0001-0.001%, quercetin weight percentage in said composition is in the range of 0.0001-0.001%, scopoletin to quercetin w/w ratio in said composition is in the range of 1:1, and said composition promotes hair growth.
[00109] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition

comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is 0.0005%, quercetin weight percentage in said composition is 0.0005%, scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1, and said composition promotes hair growth.
[00110] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1, and said composition promotes hair growth.
[00111] In an embodiment of the present disclosure, there is provided a method of promoting hair growth, said method comprising: (a) obtaining a composition comprising: (i) scopoletin; and (ii) quercetin; and (b) contacting said composition with hair follicles, wherein scopoletin weight percentage in said composition is 0.0005%), quercetin weight percentage in said composition is 0.0005%), scopoletin to quercetin w/w ratio in said composition is in the range of 1:1, and said composition promotes hair growth.
[00112] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein said composition can be for topical application. [00113] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein said composition can be in the form of an aerosol. [00114] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein said composition can be in the form of a lotion. [00115] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein said composition can be in form of an oil.

[00116] In an embodiment of the present disclosure, there is provided a composition as described herein, wherein said composition can be in the form of a serum. [00117] Although the subject matter has been described with reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the subject matter, will become apparent to persons skilled in the art upon reference to the description of the subject matter. It is therefore contemplated that such modifications can be made without departing from the spirit or scope of the present subject matter as defined.
EXAMPLES
[00118] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
Example 1
Method work-flow for processing hair follicles for proteomic analysis
[00119] In order to determine the efficacy of the composition of the present
disclosure, in promoting hair growth, hair follicles were obtained from the scalp of
healthy donors under the supervision of medical doctors at MSCR Pvt. Ltd., Banglore
after obtaining their consent, treated with and without the actives/composition,

processed and then analysed using Liquid Chromatography/Mass Spectrometry to assess the effect on hair follicle- associated proteins by the active constituents under consideration. The work- flow and methodology followed has been illustrated in Figure 1, and the methods followed at each step have been described in further detail in the following sections.
[00120] Hair follicle collection and treatment: Human hair follicles from the scalp of healthy donors were plucked using a standardized protocol by medical doctors at MSCR Pvt. Ltd., Bangalore, after obtaining their consent. Hair follicles were collected and maintained in William's E medium (Invitrogen, CA, USA) till use. Following a wash with Phosphate Buffer Saline (Phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M, pH 7.4) (Sigma) wash, the hair follicles were trimmed to about 1cm from the bulb using scissors without harming the root. Hair follicles with an intact bulb and root sheath (RS) were selected. Hair follicles were cultured in 35mm petri dish and were treated with quercetin (lOug/ml) Scopoletin (lOug/ml) and combination Quercetin + Scopoletin (5+5) ug/ml. The control was treated with dimethyl sulfoxide (DMSO). 30 follicles were selected for each set of treatment. Following treatment, the petri-dishes were incubated in a standard cell culture incubator for 48 hours. Post incubation, the follicles were washed in PBS) and collected in RNA Later (Sigma Aldrich / R0901) and samples were processed for hair follicle proteomics profiling. [001211 Hair follicle lysate preparation, In-solution protein digestion, and Desalting: The hair follicles were lysed by water bath sonication for 1 hour at 37 °C, and their proteins extracted using Mike's special lysis buffer [ 25mM Tris ( pH-7.4), 150mM Sodium chloride, 1% Triton X-100, ImM EDTA (pH-8.0), ImM Dithiothreitol, ImM PMSF (Just prior to use)] supplemented with Roche protease cocktail inhibitor complex (Catalogue Number 04693159001). The protein content in the samples were determined using Pierce BCA protein estimation kit. Equal amounts of protein were taken for in-solution protein digestion.

[00122]The proteins obtained from the previous step were subjected to an in-solution protein digestion protocol. For this, 10 ug of cell protein extract was dissolved in 47 ul of 6 M urea, 50 mM Ammonium bicarbonate solution by gentle mixing. To this, lul of 250 mM tris (2-carboxyethyl) phosphine (TCEP) was added and incubated at 37 C for 30 minutes. Further 2 ul of 625 mM iodoacetamide added and incubated in dark at room temperature for 45 minutes. Finally, in-solution digestion was performed by addition of 450 ul trypsin in 50 mM Ammonium bicarbonate with protein digest ratio of 50:1. The digested peptides were purified with OASIS 1 ml Cartridge system form Waters Scientific (WAT094225).
[00123]Nano-FtPLC & Mass Spectrometry: The purified / enriched samples were dried and re-suspended in 0.1% formic acid with 5% acetonitrile in MS water. The peptides were loaded onto an EASY-nano LC system (Proxeon). Peptide mixtures were separated on a C18 reverse phase column (PicoFrit capillary column, 75umxl0cm, New Objective) using a linear gradient of solvent A (0.1% formic acid in 5% acetonitrile) and Solvent B (0.1% formic acid in 95% acetonitrile) at a flow rate 250 nL/min directly into a LTQ-OrbitrapVelos mass spectrometer (Thermo Scientific, San Jose, CA, USA) with nano-ESI source with spray voltage was set at 1.6 KV. Stable spray condition maintained prior to sample loading. The instrument was operated in data-dependent mode with dynamic exclusion enabled. The MS/MS spectra on the top 20 most abundant peptide ions in full MS scan were obtained. The normalized collision-induced dissociation (CUD) was set at 35% for MS/MS. [00124]Raw Data Analysis: The data were processed using Proteome Discoverer, version 1.4 (Thermo Scientific TM). The files were subsequently submitted to an in-house Sequest server for database searching through the Proteome Discoverer 1.4 program. The data were searched against the human sequence database of Uniprot. The search was performed choosing trypsin as the specific enzyme. A maximum of one missed cleavage was allowed. Peptide (parent ion) tolerance of 2.5 Da and

fragment ion tolerance of 1 Da were allowed, Oxidation (M) as variable modifications and fixed modification of carbamidomethylation (C) were used.
Example 2
Results of Proteomic Analyses
[00125] A total of 1452 [Control- 389, Quercetin treated- 354, Scopoletin treated- 404 and combination (Quercetin+ Scopoletin)- 305] proteins were identified across all four samples tested as shown in Figure 2. Out of these, 912 unique proteins were identified across the set of samples, and 135 proteins were identified common across all of the samples. The common 135 proteins were further taken for quantitative data analysis to find protein markers associated with hair growth. In this set, 29 proteins were identified as significant, based on observing at least a two-fold change in the combination treatment compared to the control. Out of these, 4 proteins were of considerable interest owing to their role in hair growth modulation and also, due to the effect of the composition and actives under study. These are: Profilaggrin, Myosin-9 Superoxide dismutase [Cu-Zn], and Galectin-7, all of which are known to play key role in maintaining healthy hair structure.
[00126] Profilaggrin is encoded by the filaggrin (FLG) gene. It is involved in epidermal differentiation. It is a major component of the keratohyalin granules within epidermal granular cells.
[00127] Myosin-9 protein is a conventional non-muscle myosin. In hair and vibrissal follicles, non-muscle myosin and actin have been shown to co-localize in the outer root sheath and some hair matrix cells adjoining dermal papillae. This actin-myosin system may play a role in cell movement, differentiation, and other key functions of skin and hair cells (Kiyokazu et al., 2006, Acta Histochem. Cytochem. 39 (4): 113-123).
[00128] The Superoxide Dismutase [Cu-Zn] (SOD) protein binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the

body. Free radical-mediated damage to melanocytes of the hair follicle has been considered as a mechanism for aging of the hair. It has been shown that SOD prevents damage to melanocyte DNA by dismutating superoxide (Photochem Photobiol., 2004, 80(3):579-82).
[00129] Galectin-7 is almost exclusively expressed in stratified epithelia,
notably in the epidermis. A critical level of this protein is required for maintaining proper epidermal homeostasis.
Example 3
Effect of the Combination of Scopoletin and Quercetin on Hair Follicle Proteins
[00130] The effect of the actives scopoletin and quercetin on the four proteins identified from the proteomic dataset was analysed further. As seen in Figure 3 and Figure 4, the individual actives did not have not have any significant effect on the levels of Profilaggrin, Myosin-9, and Superoxide Dismutase, and Galectin-7 protein in hair follicles. The levels of the proteins were comparable to those seen in the control samples treated with DMSO. Therefore, it can be inferred that neither scopoletin, nor quercetin at individual concentrations of 10 ug/ml can modulate hair-follicle specific proteins.
[00131] Surprisingly however, when scopoletin and quercetin are used in combination, a highly significant increase is observed in the levels of three of the proteins identified (Figure 3). A four- fold increase in the levels of Profilaggrin, Myosin-9, and Superoxide Dismutase was observed when scopoletin and quercetin were used in combination. Each of the actives were used at individual concentrations of 5 ug/ml, which is half of the concentration of the actives used in isolation, however, the combination of the two could significantly increase protein levels, far more than the additive effect of the two.
[00132] This synergistic effect is also observed on Galectin-7 levels (Figure 4). By themselves, scopoletin and quercetin showed Galectin-7 levels comparable to those

seen in the control sample. However, in combination, scopoletin and quercetin could reduce Galectin-7 levels almost 2.5 times. Thus, the combination of the two actives could synergistically decrease hair follicle protein levels, even though half the concentration of the actives was used in the combination as compared to the individual concentrations of actives.
[00133] Thus, the combination of scopoletin and quercetin at a 1:1 ratio of 5 ug/ml scopoletin and 5 ug/ml quercetin, demonstrate a distinct synergistic effect in modulating the levels of key hair follicle- associated proteins.
[00134] Advantages of the present disclosure: Overall, the present disclosure relates to a composition comprising a combination of scopoletin and quercetin which when used individually, do not demonstrate any significant effect in the modulating hair-follicle growth factors. However, when used at a 1:1 ratio of scopoletin to quercetin, at a concentration of 5 ug/ml each, this combination can, surprisingly, significantly modulate the protein levels of these hair-follicle growth factors. This result is far more than the additive effect obtained by a mere admixture of the two actives, and therefore, the combination of scopoletin and quercetin at this particular ratio functions synergistically in augmenting the proteins involved in hair growth. Further, this specific combination of the constituents cannot be arrived at without undue experimentation and is therefore not obvious to person skilled in the art. Thus, the 1:1 combination of scopoletin and quercetin can be used in formulations for the enhancement and maintenance of the hair follicle.

I/We Claim:
1. A composition comprising:
(a) scopoletin; and
(b) quercetin,
wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:10-10:1.
2. The composition as claimed in claim 1, wherein scopoletin to quercetin w/w ratio in said composition is in the range of 1:2- 2:1.
3. The composition as claimed in claim 1, wherein scopoletin to quercetin w/w ratio in said composition is 1:1.
4. The composition as claimed in claim 1, wherein scopoletin w/w percentage in said composition is in the range of 0.0001-0.001%, and quercetin w/w percentage in said composition is in the range of 0.0001-0.001%.
5. The composition as claimed in claim 1, wherein scopoletin w/w percentage in said composition is in the range of 0.00034%)- 0.00068%), and quercetin w/w percentage in said composition is in the range of 0.00034%>- 0.00068%).
6. The composition as claimed in claim 1, wherein scopoletin w/w percentage in said composition is 0.0005%), and quercetin w/w percentage in said composition is 0.0005%.
7. The composition as claimed in any of the claims 1-6, further comprising: (a) at least one carrier; (b) at least one diluent; and (c) at least one excipient.
8. A method of preparing a composition as claimed in any of the claims 1-6, said method comprising:

(a) obtaining scopoletin;
(b) obtaining quercetin; and
(c) contacting scopoletin and quercetin to obtain said composition.
9. A method of preparing a composition as claimed in any of the claims 1-7, said
method comprising:
(a) obtaining scopoletin;
(b) obtaining quercetin;
(c) obtaining the at least one carrier;
(d) obtaining the at least one diluent;

(e) obtaining the at least one excipient; and
(f) contacting scopoletin, quercetin, the at least one carrier, the at least one diluent, and the at least one excipient to obtain said composition.
10. A method of promoting hair growth, said method comprising:
(a) obtaining a composition as claimed in any of the claims 1-7; and
(b) contacting said composition with hair follicles, wherein said composition promotes hair growth.
11. A composition as claimed in any of the claims 1-7 for use in preparing
formulations to promote hair growth.