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A Composition Of Compressed Ingredients With Enhanced Bioactivity For Cleaning And Disinfection And Method Thereof

Abstract: The present invention provides a chemical free, non-toxic composition for cleaning and disinfection comprising a synergistic composition having essential oils and terpenes as active ingredients and preservatives, emulsifiers, stabilizers and binders etc. as excipients in a right proportion, contributing to the technical advancement and synergistic activity. Due to this combination, the products shows enhanced antibacterial and antifungal properties, ensures effective cleaning on different kind of surfaces along with reduction in degree of reappearance of pigeons at their specific nesting sites, thereby reducing microbial load on the surroundings.

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Patent Information

Application #
Filing Date
25 April 2023
Publication Number
44/2024
Publication Type
INA
Invention Field
CHEMICAL
Status
Email
Parent Application

Applicants

CLENSTA INTERNATIONAL PRIVATE LIMITED
3rd Floor, Vatika Tower B, Sector 54, Golf Course Road, Gurugram-122003, Haryana, India

Inventors

1. GUPTA, Puneet
3rd Floor, Vatika Tower B, Sector 54, Golf Course Road, Gurugram-122003, Haryana, India

Specification

Description:FIELD OF INVENTION
[001] The present invention relates to a chemical-free, non-toxic, compressed ingredient based composition for cleansing and disinfection. Particularly, the present invention provides a composition for cleaning bird faeces and other animal excreta. More particularly, the present invention relates to a synergistic composition comprising essential oils and terpenes as active ingredients and excipients that ensures enhanced cleaning efficacy and reduction in microbial load in the surroundings. The composition of the present invention has antibacterial and antifungal properties.
BACKGROUND OF THE INVENTION
[002] Cleaners, disinfectants, sanitizers and other such products can be easily found in every household these days. Cleaners, disinfectants etc. are well-known for their cleaning effects, killing germs and for an inevitable role they play in controlling several communicable diseases and other infections, thereby improving human health.
[003] There are more than sixty diseases that are carried by birds and their droppings, which is a concern among humans. Residential areas are particularly vulnerable to this issue because many of the diseases are airborne and can be contracted by people just by being near birds’ droppings.
[004] Pigeons are the oldest domesticated birds in existence. They have contributed significantly to humanity by serving as messengers in the ancient times. Pigeons frequently establish their nests in older houses or other tall residential buildings, where their nests are conveniently located on building balconies, window ledges, beams, etc. However, in the current times pigeons are mainly regarded as a civic nuisance due to their droppings and are criticized for defacing the properties and monuments which lowers the aesthetic aspects of the building along with increasing the cost of maintenance manifolds.
[005] The dried droppings of pigeons are the source of spread of many deadly bacterial and fungal diseases and infections, which has a direct impact on human health. Various diseases associated with pigeon droppings includes Cryptococcosis, Histoplasmosis and Psittacosis. Humans get infected with these diseases by breathing in the dust that is created while cleaning pigeon droppings. Therefore, there is a need of an effective way to get rid of birds/pigeon infestation by warding off these species from their specific nesting sites without physically harming them or without utilizing any lethal method.
[006] Currently, several bird/ pigeon repellent products are available in the market. However, these products comprise of large quantity of harmful chemicals which are hazardous for both pigeons and human health.
[007] Reference is made to a Chinese patent application numbered CN102860300B that relates to an automatic aiming laser bird repelling device and a bird repelling method based on pattern recognition. The present application utilizes components such as camera, controller, lasers etc. which renders this system to be expensive along with energy consumption.
[008] Reference is made to a United States patent application US7867479B2 which relates to a formulation provided as a concentrated emulsion for dilution with water to repel birds wherein the formulation comprises methyl anthranilate, fatty acid and an amine such as but not limited to, monoethanolamine or triethanolamine. The fatty acid and at least some of the amine forms a surfactant to stabilize the concentrated emulsion. Further, this patent application utilizes antifoam agent. Most of these chemicals are expensive and harmful for human and animal/birds health.
[009] Thus, the existing technologies fail to disclose a chemical free, more effective, non-toxic composition for repelling bugs, insects and birds with effective cleaning efficacy and long lasting effect.
[010] Therefore, there remains a need to provide an effective and cheap chemical free, non-toxic cleaning and disinfectant composition for cleaning bird’/animals’ droppings, having antibacterial and antifungal properties so that it can provide long lasting cleaning and disinfection and also can repel bird’/animals’ from that specific area.
OBJECTIVES OF THE INVENTION
[011] The primary objective of the present invention is to provide chemicals free, non-toxic composition for cleaners and disinfectants for removing bird faeces and other animal excreta.
[012] Another objective of the present invention is to provide a synergistic composition comprising essential oils and terpenes as active ingredients and preservatives, stabilizer and emulsifiers as excipients.
[013] Yet another objective of the present invention is to provide a chemicals free, non-toxic composition for cleaners and disinfectants, ensuring personal hygiene and sanitation.
[014] Another objective of the present invention is to provide a chemical free, non-toxic composition for cleaners and disinfectants with antibacterial and antifungal properties.
[015] Another objective of the present invention is to provide a composition for cleaners and disinfectants with enhanced bioactivity.
[016] Another objective of the present invention is to provide a chemical free, non-toxic composition for cleaners and disinfectants with enhanced cleansing effect.
[017] Another objective of the present invention is to provide a chemical free, non-toxic composition for cleaners and disinfectants, ensuring reduced microbial load in the surroundings.
[018] Yet another objective of the present invention is to provide a chemical free and non-toxic bird’/animals’ repellent composition, ensuring effective cleaning along with reducing the degree of reappearance of bird’/animals’ and other domestic birds to their specific nesting sites.
[019] Another objective of the present invention is to provide a repellent solution for insects, bugs and other domestic birds and not limited to pigeons.
[020] Another objective of the present invention is to provide a ready-to-use composition for cleaning and disinfection.
[021] Another objective of the present invention is to provide a composition ensuring effective cleaning activity on different kind of surfaces.
[022] Other objects and advantages of the present invention will become apparent from the following description taken in connection with the accompanying examples to disclose the aspects of the present invention.
SUMMARY OF THE INVENTION
[023] The present invention relates to a composition for Enhanced Bioactivity of the ingredients to Disinfect the microorganisms along with the Cleansing technology (EBDC). The present invention provides a chemical-free, non-toxic composition for cleaners and disinfectants for cleaning animal droppings including bird faeces and other animal excreta from a surface. Particularly, the present invention relates to a synergistic composition comprising essential oils and terpenes as active ingredients along with preservatives, stabilizer and emulsifiers as excipients, ensuring enhanced cleaning efficacy, reduction in microbial load in the surroundings and decrease in degree of reappearance of pigeons and other domestic birds at their specific nesting sites. The composition of the present invention has antibacterial and antifungal properties.
SOURCES OF THE MATERIALS USED IN THE PRESENT INVENTION
[024] All the ingredients were procured commercially. Lemongrass oil and Eucalyptus oil were procured from AG Industries, Noida. Alpha Terpinene was purchased from TCI Chemicals and Polysorbate 80 and Potassium Sorbate were purchased from SRL Chemicals.
DETAILED DESCRIPTION OF THE INVENTION
[025] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure.
[026] Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope of the invention. In addition, descriptions of well-known functions and constructions are omitted for clarity and conciseness.
[027] Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.
[028] The terms and words used in the following description and claims are not limited to the bibliographical meanings but are merely used to enable a clear and consistent understanding of the invention. Accordingly, it should be apparent to those skilled in the art that the following description of exemplary embodiments of the present invention are provided for illustration purpose only and not for the purpose of limiting the invention.
[029] It is to be understood that the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise.
[030] It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, integers, steps, or components but does not preclude the presence or addition of one or more other features, integers, steps, components, or groups thereof.
[031] The present invention relates to a chemical-free, compressed, non-toxic cleaning and disinfectant composition for cleaning bird faeces and other animal excreta from a surface. Particularly, the present invention relates to a synergistic composition having Enhanced Bioactivity of the ingredients to Disinfect the microorganisms along with the Cleansing technology (EBDC). The synergistic composition comprises essential oils and terpenes as active ingredients along-with preservatives, stabilizer and emulsifiers as excipients, ensuring enhanced cleaning efficacy, reduction in microbial load in the surroundings and decrease in degree of reappearance of pigeons and other domestic birds at their specific nesting sites. The composition of the present invention has antibacterial and antifungal properties and is completely sage and non-toxic.
[032] The chemicals free, non-toxic composition for cleaners and disinfectants comprises of:

a. a plurality of essential oils 30-50%
b. at least one terpene 5% - 15%
c. at least one preservative 1% - 21.11%
d. at least one emulsifier 17.78% - 37.78%
e. at least one binder 1% - 21.11%

[033] In an exemplary embodiment, the essential oil is a compound selected from a group comprising of lemongrass oil, eucalyptus oil or a combination thereof.
[034] In an exemplary embodiment, terpene is alpha terpinene.
[035] In an exemplary embodiment, the preservative is potassium sorbate.
[036] In an exemplary embodiment, the emulsifier is polysorbate 80.
[037] In an exemplary embodiment, the binder is water.
[038] In an exemplary embodiment, the solvent used in the present invention is water.
[039] In a preferred embodiment, the composition of the present invention provides antibacterial and antifungal properties, thereby ensuring personal hygiene and sanitation.
[040] The composition of the present invention shows enhanced cleaning effect on different kind of surfaces along with reduction in degree of reappearance of pigeons and other domestic birds to their nesting sites, thereby reducing microbial load in the surroundings.
[041] The composition of the present invention may be used as repellent to other insects, bugs and other birds such as, but not limited to pigeons.
[042] The present invention also provides a process of preparation of the chemical free, non-toxic composition for cleaners and disinfectants comprising the steps of:
a. preparing a solution of preservative in luke warm water;
b. preparing a mixture of one or more active ingredients comprising one or more essential oil and terpene , wherein the weight ratio of the essential oils and terpene is in a ratio in the range of 8:1;
c. adding emulsifier to the mixture of active ingredients of step 1 in a ratio of 2:9;
d. adding the solution formed in step (a) to the solution formed in step (c), forming the desired composition such that amount of preservative: active ingredients is in a ratio of 2:9 .
[043] The composition of the present invention is used as spray over the areas affected by animal droppings. The area is first scrapped off and thereafter the composition is sprayed all over the area where the droppings are found, for instance.
[044] In an exemplary embodiment, Table 1 illustrates the ingredients of the chemical free, non-toxic composition along with the amount of each ingredient

Ingredients
% Composition (w/w)
Lemongrass oil 22.22%
Eucalyptus oil 22.22%
Alpha Terpinene 5.56%
Polysorbate 80 27.78%
Potassium sorbate 11.11%
Water 11.11%

[045] The following embodiment provides examples of preparing the composition and should not be construed to be limiting the scope of the present invention.
[046] EXAMPLE 1
[047] Table 2 illustrates the compounds and amount thereof for preparing a chemical free, non-toxic composition for cleaners and disinfectants

Ingredients
% Composition (w/w) Quantity (in g) for 30 ml Sachet Function
Lemongrass oil 22.22% 6.66 Active Ingredient
Eucalyptus oil 22.22% 6.66 Active Ingredient
Alpha Terpinene 5.56% 1.66 Active Ingredient
Polysorbate 80 27.78% 8.33 Emulsifier
Potassium sorbate 11.11% 3.33 Preservative
Water 11.11% 3.33 Binder
Total 100.00% 30

[048] Method of preparation of formulation
[049] To prepare a concentrated formulation of a chemical free, non-toxic composition for cleaners and disinfectants, a solution of 11.11g of potassium sorbate is prepared in luke warm water. Further, a mixture of lemongrass oil (22.22g), eucalyptus oil (22.22g) and alpha terpinene (5.56g) is prepared in which polysorbate 80 is weighed 27.78g and added to prepare a batch. Lastly, the solution of potassium sorbate prepared at first step is added to this batch at room temperature and approximately 1 atm pressure of under constant stirring to form the formulation.
[050] EXPERIMETAL DATA
[051] EFFICACY OF THE COMPOSITION OF THE PRESENT INVENTION: The composition of the present invention has enhanced cleansing effect along with reducing the degree of reappearance of pigeons at their nesting sites. The composition offers enhanced antimicrobial and antifungal properties.
[052] The following tests were performed for determining efficacy of the composition of the present invention:
[053] Antibacterial study
[054] The Minimum Inhibitory Concentration (MIC) of an antibacterial is the minimum concentration that inhibits complete growth of bacteria.
[055] The method of antibacterial study is based on the inoculation of a standardized liquid culture of the test strain in a dilution series of the tested antibacterial for which the MIC is determined. The first concentration in the dilution series at which no visual growth can be determined is then considered as the MIC.
[056] Microorganisms Used
[057] The microorganisms used for this study were Escherichia coli (ATCC 8739) and Salmonella typhimurium (ATCC 23564) of bacterial strain type.
[058] Preparation of stock spore suspension of test organisms:
[059] Bacterial cultures- The working culture of test organism (E.coli and S. typhimurium) was prepared. Stock solution of the test organism was prepared as to obtain a count of at least 5 x 105 cfu/ ml by using densitometer.
[060] Test Procedure:
[061] Antibacterial activity-The concentration of formulation of the present invention taken was 100 mg/ml to 10 mg/ml. The concentration of sample was prepared in Soybean Casein Digest Medium (SCDM). After preparation of all the tubes, a 100µl of the adjusted culture (approx.105cfu/ml) was taken in to each tube and all the tubes were incubated for 24 hours. After incubation, all the tubes were visually observed for growth of microorganisms. The concentration at which the growth of test organisms is inhibited was considered as the MIC of product.
[062] Calculation
The effectiveness of the antibacterial activity of the given sample was recorded in terms of MIC. Graph was plotted with respect to log concentration of the test product and probit of mortality.
[063] Result
[064] The antibacterial activity of the formulation of the present invention was calculated in terms of LC50 against Escherichia coli and Salmonella typhimurium. It was evaluated to be 47.86 mg/ml and 50.11 mg/ml respectively.
[065] Antifungal Study
[066] The Minimum Inhibitory Concentration (MIC) of an antifungal is defined as the minimum concentration that inhibits complete growth of fungus.
[067] The method is based on the inoculation of a standardized liquid culture of the test strain in a dilution series of the tested antifungal for which the MIC is determined. The first concentration in the dilution series at which no visual growth can be determined is then considered as the MIC. Graph was plotted with respect to log concentration of the test product and Probit of mortality for calculation of LC50.
[068] Microorganisms used
[069] The microorganisms used for this study were Cryptococcus neoformans (NCIM 3378), Candida albicans (ATCC 10231) and Saccharomyces cerevisiae (ATCC 9763) of fungal strain type.
[070] Preparation of stock spore suspension of test organisms:
[071] Fungal cultures-
[072] The working culture of test organism (Cryptococcus neoformans, Candida albicans and Saccharomyces cerevisiae was prepared. Stock solution of the test organism was prepared as to obtain a count of at least 0.5-2.5 x 103 cfu /ml by using densitometer.
[073] Test Procedure:
[074] Antifungal activity-
[075] The concentration of formulation of the present invention taken was 100mg/ml to 10 mg/ml. The concentration of sample was prepared in SCDM. After preparation of all the tubes, a 100µl of the adjusted culture (approx.103 cfu/ml) in to each tube and incubate all the tubes for 72 hours. After incubation, all the tubes were visually observe for growth of microorganisms. The concentration at which the growth of test organisms is inhibited will be considered as the MIC of product.
[076] Calculation
[077] The effectiveness of the antifungal activity of given sample was recorded in terms of MIC. Graph was plotted with respect to Log concentration of the test product and Probit of mortality.
[078] Result
[079] The antifungal activity of the given formulation was calculated in terms of LC50 against Cryptococcus neoformans, Candida albicans and Saccharomyces cerevisiae. It was observed as 50.11 mg/ml, 50.11 mg/ml and 47.86 mg/ml respectively.
[080] Cytotoxicity Test
[081] EXPERIMENTAL PROCEDURE
[082] Sterility of Test Item-
The test item was handled aseptically throughout the test procedure.
[083] Media Used-
Serum-supplemented DMEM cell culture media was used.
[084] Control Used-
1) Culture Medium containing 10% FBS (Negative control)
2) Liquefied Phenol (Positive control) - 1.6 mg/ml in culture media
[085] PREPARATION OF CELL MONOLAYER
[086] The L-929 cell lines were maintained in DMEM medium containing 10% fetal bovine serum and 1% antibiotics (Penicillin-streptomycin). When the cells were nearly 80% confluent, media was aspirated from cell culture flask and the cells were rinsed with sterile phosphate buffer saline. 1.0 ml trypsin solution was added into the flask and incubated for 2 minutes at room temperature to detach the cells. After 2 minute 10 ml media was added immediately for neutralizing the effect of trypsin and centrifugation was done at 1500 rpm for 10 minutes. The supernatant was discarded and the cell pellet was resuspended in 10 ml of fresh media (1 X 105 cells/ml). 100µl of cell suspension (1 X 104 cells/well) was transferred into each well of 96 well culture plates. The culture plates were kept at 37±10° C in an incubator containing 5±1% of carbon dioxide until a near 80% confluent cell monolayer was formed as observed by microscopic examination.
[087] Method:
[088] A multiple culture of L-929 (ATCC cell lines) mouse fibroblast cells was used. The minimum essential cell culture medium was prepared without fetal calf serum containing antibiotics. The medium was later supplemented with 10 % fetal calf serum and filtered sterilized. The medium was aspirated from cell culture flask containing a near confluent cell monolayer. The cells were rinsed twice with phosphate buffer saline & then the phosphate buffer saline was decanted. 1.0 ml of Trypsin solution was added into the flask. The cells were incubated for 2 minutes at room temperature to suspend the cells. After that 10 ml media was added for neutralizing the effect of trypsin and then cell suspension was transferred into 15 ml centrifuge tube and was centrifuged for 5 minutes at 700 rpm. The supernatant was discarded and the cell pellet was re-suspended in 10 ml of fresh medium and mixed thoroughly. 100µl Cell suspension was added in each well of 96 well plate. These plates were incubated at 37 ± 1° C in an incubator containing 5 ± 1% Carbon dioxide atmosphere until a near-confluent monolayer was formed, as observed and examined by microscopic examination. The culture medium was taken out and replaced with Fresh serum-supplemented culture medium containing four different concentrations of test item, the positive control and negative control. All the cultures were in triplicates and incubated for 24 ±1 hrs in CO2 incubator. After 24 hours, microscopic evaluation of morphological alteration were observed and the media in plate was replaced with 50 µl MTT solution (1mg/ml) and incubated for 2hr. The MTT solution was decanted from each well and 100 µl of isopropanol was added to each of test and control wells. Plate was swayed and optical density/absorption was read at 570 nm.
[089] Interpretation of Results:
[090] The confluency of the monolayer was recorded as (+) if present and (-) if absent. Each culture well was evaluated for viability percent by MTT Cytotoxicity test. To calculate the reduction of viability compared to control, Equation is used:
Viab. % = 100 x OD570e\OD570b
Where,
OD570e – Absorbance of the 100% extract of test sample
OD570b – Absorbance of the control
The Lower the viability %, the higher the cytotoxic potential of the test item.
If viability will be reduced <70% of the blank, it has a cytotoxic potential.
[091] Result:
[092] The test item “one sample of formulation of the present invention” was found to be noncytotoxic at dose concentration of 2mg/ml when viability % and cellular characteristics of cells were compared with negative control. The individual reactivity grades and viability % are shown in Table 3 & 4.
[093] Conclusion:
[094] Under the condition of this study, the viability % of L929 cells treated with test item ‘formulation of the present invention’ at dose concentration 2mg/ml, came out as > 70 % when compared to its control counterpart. Therefore, the dose concentration 2mg/ml was found to be Non- Cytotoxic.
[095] Table 3 illustrates observation of obtained scores
Sample Observation Grade Reactivity Inference
Positive
control
Cells were rounded off, their appendages were withdrawn. 4 Severe Severely-
Cytotoxic
Negative
Control Cells were well in shape and morphology with no reactivity. 0 None Non-Cytotoxic
Test
Sample
(2mg/ml) Cells were well in shape and morphology with no reactivity. 0 None Non-Cytotoxic

[096] Table 4 illustrates calculation of viability percentage
S. No. Sample Mean O.D % viability
1. Positive control 0.050 4.07
2. Negative control 1.228 100.00
3. Test sample(8mg/ml) 0.22 17.92
4. Test sample(4mg/ml) 0.392 31.92
5. Test sample(2mg/ml) 1.055 85.91
6. Test sample(1mg/ml) 1.169 95.20

[097] Different variations of ingredients were prepared with different percentage composition and checked for the Pigeon repellence.
[098] The study was performed at the Pigeon roosting sites, where an area of around 1000 sq. inches was selected. The average temperature is 31.4°C with humid conditions as 64% and pressure as 921 millibar. The following protocol was used to calculate the percentage of pigeon repellency:
a. The Pigeon roosting sites were cleaned thoroughly.
b. The prescribed composition was sprayed on the site, around 18 to 20 sprays or 8-9 mL was sprayed.
c. It was sprayed daily till first 15 consecutive days, then thrice a week, then twice a week and then once a week for a total of 5 weeks.
d. The appearance of Pigeons or the Pigeon dropping was observed and compared to the control samples.
e. Based on the raw data a correlation is been established between the number of days and the Pigeon appearance (or pigeon droppings) as shown in Table 3.
[099] Table 5 illustrates Pigeon Repellency data
Area of spray (sq. inch) 1000 Volume of repellent used 8-10 mL
No. of Day Date No. of spray Temperature Pressure (mb) Humidity Time of observation No. of dropping
Day 0 16-09-19 11:30 AM 17
Day 0 16-09-19 0 32.8 1001 61% 6:30 PM 18

Day 1 17-09-19 11:30 AM 18
Day 1 17-09-19 20 34.4 918 56% 6:20 PM 16

Day 2 18-09-19 11:15 AM 10
Day 2 18-09-19 24 33.4 918 55% 6:15 PM 9

Day 3 19-09-19 11:15 AM 8
Day 3 19-09-19 20 30.8 917 63% 6:15 PM 7

Day 4 20-09-19 11:15 AM 4
Day 4 20-09-19 18 32.4 922 61% 6:15 PM 5

Day 5 21-09-19 11:15 AM 4
Day 5 21-09-19 22 31.7 926 58% 6:15 PM 3

Day 6 22-09-19 11:15 AM 3
Day 6 22-09-19 20 31.4 920 56% 6:15 PM 2

Day 7 23-09-19 11:15 AM 1
Day 7 23-09-19 20 30.2 922 66% 6:15 PM 2

Day 8 24-09-19 11:15 AM 1
Day 8 24-09-19 21 32.6 924 68% 6:15 PM 0

Day 9 25-09-19 11:15 AM 0
Day 9 25-09-19 22 29.6 923 65% 6:15 PM 1

Day 10 26-09-19 11:15 AM 0
Day 10 26-09-19 20 29.8 922 66% 6:30 PM 0

Day 11 27-09-19 11:15 AM 0
Day 11 27-09-19 24 30.2 920 72% 6:30 PM 0

Day 12 28-09-19 11:15 AM 0
Day 12 28-09-19 24 29.9 922 70% 6:30 PM 0

Day 13 29-09-19 11:20 AM 0
Day 13 29-09-19 20 30.6 920 68% 6:15 PM 0

Day 14 30-09-19 11:10 AM 0
Day 14 30-09-19 18 30.4 922 70% 6:30 PM 0
Average 31.4 926.8 64%

[100] Conclusion
[101] The present invention is effective in repelling pigeons within 10 days when sprayed daily. The pigeon droppings were observed daily before and after spraying and it has been observed that the number of droppings decreased day by day and there were no droppings on 9th day, therefore no pigeon reappeared at the nesting site.
[102] The present invention provides the following advantages:
(a) The composition of the present invention comprises active ingredients in combination with an effective amount of preservative and other excipients, ensuring enhanced bioactivity.
(b) The formulation exhibits a surprisingly good antibacterial activity against widely known disease-causing bacteria and fungi found in the excretion of birds.
(c) The composition is utilized to repel bugs, insects and other domestic birds including pigeons and to avoid diseases caused by pigeon droppings.
, Claims:WE CLAIM:
1. A composition with enhanced bioactivity for cleaning and disinfection comprising of:
a. a plurality of essential oils from 12.22% to 32.22% by weight of the total composition (wt/wt);
b. At least one terpene from 5% to 15% (wt/wt);
c. at least one preservative from 1% to 21.11% (wt/wt);
d. at least one emulsifier from 17.78% to 37.78% (wt/wt); and
e. at least one binder from 1% to 21.11% (wt/wt);
2. The composition as claimed in claim 1, wherein the essential oil is selected from a group comprising of lemongrass oil and eucalyptus oil or a combination thereof.
3. The composition as claimed in claim 1, wherein the terpene is alpha terpinene.
4. The composition as claimed in claim 1, wherein the preservative is potassium sorbate.
5. The composition as claimed in claim 1, wherein the emulsifier is polysorbate 80.
6. The composition as claimed in claim 1, wherein the binder is a solvent.
7. The composition as claimed in claim 1, wherein the solvent is water.
8. The composition as claimed in claim 1, wherein the composition is chemical free, non-toxic, with enhanced antibacterial and antifungal properties, ensuring effective cleaning on different kind of surfaces along with reduction in degree of reappearance of pigeons at their specific nesting sites, thereby reducing microbial load on the surroundings.

9. The composition as claimed in claim 1 is utilized to repel bugs, insects and other domestic birds.

Documents

Application Documents

# Name Date
1 202311029852-STATEMENT OF UNDERTAKING (FORM 3) [25-04-2023(online)].pdf 2023-04-25
2 202311029852-POWER OF AUTHORITY [25-04-2023(online)].pdf 2023-04-25
3 202311029852-OTHERS [25-04-2023(online)].pdf 2023-04-25
4 202311029852-FORM FOR STARTUP [25-04-2023(online)].pdf 2023-04-25
5 202311029852-FORM FOR SMALL ENTITY(FORM-28) [25-04-2023(online)].pdf 2023-04-25
6 202311029852-FORM 1 [25-04-2023(online)].pdf 2023-04-25
7 202311029852-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [25-04-2023(online)].pdf 2023-04-25
8 202311029852-DECLARATION OF INVENTORSHIP (FORM 5) [25-04-2023(online)].pdf 2023-04-25
9 202311029852-COMPLETE SPECIFICATION [25-04-2023(online)].pdf 2023-04-25
10 202311029852-Proof of Right [02-05-2023(online)].pdf 2023-05-02