This invention relates to a method of herbal anti-aging (antioxidant) potential extract of allium przewalskanium.
Background of the Invention
EP3847897A1 Composition for making a tea beverage or herbal and vegetable broths- the present invention relates to an infusion product for making a beverage, more specifically to a plant-based composition for making a beverage, and to a herbal and/or vegetable composition or bouquet garni. The plants are fruits, herbs, medicinal plants, tea, vegetables and/or spices. In particular, the present invention relates to a fiber-web, preferably a tea bag, made from said fruits, herbs, medicinal plants, tea, vegetable and/or spices
Research Gap: In this patent use of selected plant for making herbal tea but in our invention involves for anti-diabetic purpose No use of extract of Allium przewalskanium involved in it but
US8993008B1 Herbal composition for treating diabetes disclose the herbal composition for treating diabetes is a mixture of medicinal herbs, including gymnema (Gymnema sylvestre), bilberry (Vaccinium myrtillus), Asian ginseng (Panax ginseng), fenugreek (Trigonella foennum-graecum), marshmallow (Althaea officinalis), “true cinnamon” (also known as “Ceylon cinnamon” or “Sri Lanka cinnamon”) (Cinnamomum verum), bitter melon (also known as “bitter gourd”) (Momordica charantia), autumn crocus (also known as “meadow saffron”) (Colchicum autumnale), bay laurel (Laurus nobilis), colocynth (Citrullus colocynthis) and prickly pear (also known as “cactus pear”) (Opuntia ficus-indica). Preferably, the herbal components of the above composition are dried, ground and packaged in a teabag or the like, allowing the composition to be delivered to the patient as an aqueous extract, similar to a conventional herbal tea. Preferably, about 5 grams of the composition are contained in the teabag, which is steeped in about 150 mL of boiling water for between about three minutes and about five minutes.
Research Gap: In this they used extract and parts of gymnema (Gymnema sylvestre), bilberry (Vaccinium myrtillus), Asian ginseng (Panax ginseng), fenugreek (Trigonella foennum-graecum), marshmallow (Althaea officinalis), for antidiabetic formulation but they have not taken extract of Allium przewalskanium for antidiabetic and antioxidant purpose
Medicinal plants are the fundamental unit of traditional medicine system in Nepal. Nepalese people are rich in traditional medicine especially in folk medicine (ethnomedicine), and this system is gaining much attention after 1995. The use of medicinal plants has increased during the COVID-19 pandemic as a private behavior (not under the control of government). A lot of misinterpretations of the use of medicinal plants to treat or prevent COVID-19 have been spreading throughout Nepal which need to be managed proactively. In this context, a research was needed to document medicinal plants used, their priority of use in society, their cultivation status, and the source of information people follow to use them. This study aimed to document the present status of medicinal plant use and make important suggestion to the concerned authorities.
Research Gap: This Res paper is on use of plant extract for treatment of Covid-2019 but not for anti-diabetic purpose
None of the prior art indicate above either alone or in combination with one another disclose what the present invention has disclosed. Present invention is a method of herbal anti-aging (antioxidant) potential extract of Allium Przewalskanium.
SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts, in a simplified format, that are further described in the detailed description of the invention.
This summary is neither intended to identify key or essential inventive concepts of the invention and nor is it intended for determining the scope of the invention.
To further clarify advantages and features of the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.
Diabetes mellitus is a complex metabolic hormonal disorder associated with hyperglycemia. It is resulted from either insulin insufficiency or deficient and scientific reports revealed that diabetes cannot be cured completely. Type-1 and type-II are two types of diabetes. Type-1 is develoed due to genetically disorder while type-2 is caused mainly due to life style of people. About 10% of the population all over the world are suffering from Diabetes mellitus and it is a chief health problem to urban population.
Discloses herein A composition of herbal anti-aging (antioxidant) potential extract of Allium Przewalskanium comprises Extracts ( ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-oxidant /antigaing purpose; Extracts ( ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-diabetic purpose.; Leaves, stem, roots or flowers ,all parts of selected plant are used for antidiabetic as well as for providing antioxidant properties. Allium Przewalskanium plant parts like flower, leaves stem roots are collected from; and the plant was taxonomically identified with flora of Aerial parts of the plants were taken and washed with distilled water; Materials are first chopped into small pieces and air-dried in shade for 15 days at room temperature (28oC to 30oC); the dried materials of Allium hypsistum are ground to get a powder which was sieved with a mesh screen.
In this invention extract of selected nepali jimbu plany i.e. Allium hypsistum have been used for controlling and treating type-II diabetes and found effective in mice experiment.
DETAILED DESCRIPTION OF THE INVENTION
The detailed description of various exemplary embodiments of the disclosure is described herein with reference to the accompanying drawings. It should be noted that the embodiments are described herein in such details as to clearly communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present disclosure as defined by the appended claims.
It is also to be understood that various arrangements may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, are intended to encompass equivalents thereof.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms “a",” “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “comprises,” “comprising,” “includes” and/or “including,” when used herein, specify the presence of stated features, integers, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groups thereof.
It should also be noted that in some alternative implementations, the functions/acts noted may occur out of the order noted in the figures. For example, two figures shown in succession may, in fact, be executed concurrently or may sometimes be executed in the reverse order, depending upon the functionality/acts involved.
In addition, the descriptions of "first", "second", “third”, and the like in the present invention are used for the purpose of description only, and are not to be construed as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include at least one of the features, either explicitly or implicitly.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
These and other advantages of the present subject matter would be described in greater detail with reference to the following figures. It should be noted that the description merely illustrates the principles of the present subject matter. It will thus be appreciated that those skilled in the art will be able to devise various arrangements that, although not explicitly described herein, embody the principles of the present subject matter and are included within its scope.
This invention involves explore of extracts of various parts of Allium Przewalskanium for antioxidant as well as antidiabetic purpose. Allium Przewalskanium is a species of high altitude in himalyan regions, it is in the Amaryllis family found in hill areas of India and Nepal.
Allium Przewalskanium is a herbal plant which is less explored for medicinal values directly however in in rural villages of Nepal it is being used for some local medicine..
Researcher has not done work on extracts of Allium Przewalskanium for antioxidant as well as for antidiabetic potential in its extract, no work done reported on alcoholic extract extraction from leaves or flowers or roots and use for antioxidant and antidiabetic purpose.
In this work Allium przewalskanium was chosen for antioxidant and antidiabetic activity in streptozotocin induced diabetic mice. Streptozotocin is an alkylating antineoplastic agent which selectively destructs insuling producing beta cells of the pancrease in mammals. As a result, blood glucose level is raised causing chronic hyperglycaemia,
Leaves, stem and root samples were collected from Nepal region and after airdrying extracts were taken by solvent extraction technique in different polar, non polar and semi polar solvents like aquesous extract, alcoholic extract, n-hexane extract and other extracts.
Different Extractsof of Allium Przewalskanium and other two allium species were r tested for antidiabetic and antioxidant activities in streptozotocin induced diabetic mice with the approval of animal house. Streptozotocin is an alkylating antineoplastic agent which selectively destructs insuling producing beta cells of the pancrease in mammals. As a result, blood glucose level is raised causing chronic hyperglycaemia. Alcoholic extract of this plant has shown a much better imact on selected mice as antidiabetic agent. Which is comparably as good as synthetic antidiabetic agent.
Best Method of working:
Material and Methods
Plant extracts
Allium Przewalskanium plant parts like flower, leaves stem roots were collected from Mustang district and Gorkha district of Nepal. The plant was taxonomically identified with flora of Nepal provided by the Department of Plant Resources, National Herbarium and Plant Laboratories, Govt. of Nepal. Aerial parts of the plants were taken and washed with distilled water. Materials were first chopped into small pieces and air-dried in shade for 15 days at room temperature (28oC to 30oC) The dried materials of Allium hypsistum were ground to get a powder which was sieved with a mesh screen. After that, extraction of the powdered materials was carried out separately by the soxhlet method using ethanol, n-hexane, and distilled water. Then, tinctures were fully dried and free from solvents by evaporating at 400C and kept in separate containers with proper labels. They were stored in the refrigerator at 4oC until further use.
Antioxidant( Anti-aging) Method
Total phenolics content and DPPH free radical scavenging assay
Total phenolics content (TPC) and DPPH free radical scavenging assay in selected plants were summarized in table 2. From the table, it is evident that A przewalskanium had higher TPC in each of the solvent extracts compared to that of and A wallichii . More precisely, the highest phenolics content was observed in ethanol extraction
Animals
Healthy and adult albino mice weighing 29 to 38 g and age of 8 to 10 weeks were taken for the study. They were fed with normal diet and kept in standard condition at room temperature of 25±10C following 12 hours light and 12 hour dark cycle. Animal caring and handling were carried out throughout the experiment according to guide for the care and use of laboratory animals (13).
Grouping and dosing of animals
Total 72 albino mice were grouped into twelve groups including six mice in each group. They were categorized as; one normal control, one diabetic control, one diabetic reference and nine test groups. After overnight fasting, freshly prepared 150mg/kg streptozocin (STZ) in 0.1M citrate buffer (pH 4.5) dose were administered via intraperitoneal route to all mice except normal control group. After three days (72 hours), the mice were evaluated for diabetes mellitus by estimating blood glucose level piercing lateral tail vein using Glucometer (Dr. Morepen, India).
Normal control group was designed as negative control (no diabetes) and given distilled water; the next diabetic control group was a positive control (diabetic induced) and normal diet; the third group served as diabetic reference i.e. diabetic induced and administered orally with 500mg/kg dose of reference drug metformin; the remaining diabetic induced groups (group 4th to 12th) were orally administered with 500mg/kg dose of plant extracts- ethanol, water and hexane extracts of selected plant; A. hypsistum, The blood glucose level were estimated after overnight fasting, at 72hours (baseline) after STZ administered and after 7th and 14th day of baseline
Statistics
The obtained data were analyzed statistically in Microsoft Excel (windows 10) and SPSS (version 25). The result of experiment was expressed as mean ± SD.
Results and Discussion
Change in body weight of diabetic mice
Loss of body weight and increase in blood glucose level indicate induction of diabetes which was seen in this study. Diabetes was induced by streptozotocin (STZ) in albino mice. From Table1, the diabetic control (DC) group showed reduction in body weight of albino mice after 72hrs (baseline day) of STZ administration and gradually reduced the body weight from baseline average 31.93±1.04g to 30.75±0.78g at 7th and 28.85±1.46g at 14th day. In contrast, the normal control (NC) group showed slight increased in body weight of mice from baseline average 36.15±0.92g to 37.52±0.77g at 7th day and 37.52±0.77g at 14th day which group did not receive the STZ. The average body weight of all group mice were found to be decreased significantly after 72hrs of STZ administration. Then, repeated daily exposure of metformin (reference drug) dose showed increment in body weight at 7th and 14th days gradually. Among all plant extracts, repeated daily dose exposure of ethanol extracts of A. hypsistum (DHe) showed remarkable increment in the body weight from baseline average 30.82±0.17g to 33.43±0.23g at 7th and 35.08±0.47g at 14th days. Moreover, percent increase in body weight was 8.47% at 7th day and 13.82% at 14th day for ethanol extract of A. hypsistum which are more weight reduction than showed by that of reference metformin. Similarly, water and hexane extracts of A. hypsistum were found to be effective gaining the weight of exposed diabetic mice. Comparatively, extracts of A hypsistum showed more effective in weight reduction of diabetic mice than other extracts.
Alcoholic extract was found more potent in weight reduction of diabetic mice than water extracts and hexane extract.
Streptozotocin induced diabetic mice are extensively used laboratory animal models to evaluate the antidiabetic properties of herbs or plants. Glibenclamide or metformin is often used standard antidiabetic drug to compare antidiabetic activity of medicinal plants. Body weight loss after STZ administration is a major symptom of diabetic mammals which could be due to insufficient supply of energy fuel to the body resulting excessive burning of fat and tissue protein for energy.. In present invetnion, body weight of mice was reduced after STZ administration which was then improved after repeated daily dose of selected plant extracts and metformin.
Anti-diabetic activities of selected plant extracts
The effect of plant extracts in STZ induced diabetic mice is shown in attached results of animal house. The fasting glucose levels in the diabetic mice were in the range of 290- 350 mg/dl. In the study, NC group was considered as normal control mice since they were not exposed with STZ and were non-diabetic group. The fasting glucose levels were markedly dropped gradually at 7th and 14th day with exposure of repeated daily dose of plant extracts. Comparing with reference drug group (DR), ethanol extract of this plant showed closed percent reduction in blood glucose level than showed by other plant extracts. DHe ( i,e ethanolic extract) has shown antidiabetic property comparable to standard reference.
Fasting blood glucose level (mg/dl) % Reduction
Groups Baseline 7th Day 14th day 7th day 14th day
NC 84.20 ± 4.1 82.10 ± 3.30 81.20 ± 2.03 2.10 3.00
DC 295.10 ± 9.25 310.1 ±11.40 316.80 ± 8.20 -4.60 -6.70
DR 320.10 ± 10.20 165.66 ±9.10 145.5 ± 7.20 48.4 54.50
Table 2: Anti-hyperglycemic activity of repeated daily doses of the plant extracts in diabetic mice.
Values are mean ± SD; n=6 for each group.

Table- Antioxidnat Propoerty In Allium species ( comparative)
; Result of total phenolics content and DPPH free radical scavenging assay in selected plants

Plants Total phenolic content (mgGAE/100g) DPPH free radical scavenging assay (% RSA)
ethanol extract n-hexane extract aqueous
extract ethanol extract n-hexane extract aqueous
extract
A. przewalskanium 122 44 82 39.53 39.44 49.26
A. wallichii 102 12 72 55.20 35.02 48.11
*Ascorbic acid was used as a reference compound which % RSA was found to be 75.11
ADVANTAGES OF THE INVENTION:
New extracts of selected plant Allium Przewalskanium can be produced in ethanolic solvent and its extract can be used for controlling and treatment of diabeties and also as anti-aging ( antioxidant) agent.
1. Extracts ( ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-oxidant /antigaing purpose
2. Extracts ( ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-diabetic purpose.
3. Leaves, stem, roots or flowers ,all parts of selected plant are used for antidiabetic as well as for providing antioxidant properties.

We Claim:

1. A composition of herbal anti-aging (antioxidant) potential extract of Allium Przewalskanium comprises Extracts (ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-oxidant /antigaing purpose; Extracts (ethanolic, aquesous and other solvent) of Allium Przewalskanium is used for anti-diabetic purpose.; Leaves, stem, roots or flowers, all parts of selected plant are used for antidiabetic as well as for providing antioxidant properties.
2. The composition as claimed in claim 1, wherein Allium Przewalskanium plant parts like flower, leaves stem roots are collected from; and the plant was taxonomically identified with flora of Aerial parts of the plants are taken and washed with distilled water; Materials are first chopped into small pieces and air-dried in shade for 15 days at room temperature (28oC to 30oC); the dried materials of Allium hypsistum are ground to get a powder which is sieved with a mesh screen.
3. The composition as claimed in claim 1, wherein after that, extraction of the powdered materials was carried out separately by the soxhlet method using ethanol, n-hexane, and distilled water. Then, tinctures were fully dried and free from solvents by evaporating at 400C and kept in separate containers with proper labels; they are stored in the refrigerator at 4oC until further use.
4. The composition as claimed in claim 1, wherein Allium przewalskanium is chosen for antioxidant and antidiabetic activity in streptozotocin induced diabetic mice; and Streptozotocin is an alkylating antineoplastic agent which selectively destructs insuling producing beta cells of the pancrease in mammals; as a result, blood glucose level is raised causing chronic hyperglycaemia.
5. The composition as claimed in claim 1, wherein leaves, stem and root samples are collected and after airdrying extracts are taken by solvent extraction technique in different polar, non-polar and semi polar solvents like aquesous extract, alcoholic extract, n-hexane extract and other extracts.
6. The composition as claimed in claim 1, wherein Different Extractsof of Allium Przewalskanium and other two allium species were r tested for antidiabetic and antioxidant activities in streptozotocin induced diabetic mice with the approval of animal house.
7. The composition as claimed in claim 1, wherein Streptozotocin is an alkylating antineoplastic agent which selectively destructs insuling producing beta cells of the pancrease in mammals; aAs a result, blood glucose level is raised causing chronic hyperglycaemia. Alcoholic extract of this plant has shown a much better imact on selected mice as antidiabetic agent.
8. A method of preparation of composition as claimed in claim 1-7, wherein the method comprising the steps of: Allium Przewalskanium plant parts like flower, leaves stem roots are collected from; and the plant is taxonomically identified with flora of Aerial parts of the plants were taken and washed with distilled water; Materials are first chopped into small pieces and air-dried in shade for 15 days at room temperature (28oC to 30oC); the dried materials of Allium hypsistum are ground to get a powder which was sieved with a mesh screen.