Claims:We claim:

1. A cosmetic formulation for enhancing moisturization, comprising:
a solvent;
a predefined quantity of a chelating agent;
a predefined quantity of a plurality of humectants;
a predefined quantity of a solubilizer;
a predefined quantity of a fragrant substance;
characterized in that
a filtrate obtained by fermenting carrot root using a bacillus culture, for enhancing the moisturization;
a first mixture obtained by stirring the solvent and the chelating agent;
a second mixture obtained by adding the humectants to the first mixture while continuously stirring;
a first phase mixture obtained by adding a predefined quantity of the filtrate to the second mixture while continuously stirring;
a second phase mixture obtained by mixing the solubilizer and the fragrant substance while continuously stirring; and
the cosmetic formulation obtained by adding the second phase mixture to the first phase mixture.
2. The cosmetic formulation as claimed in claim 1, wherein a pH adjuster added to the cosmetic formulation for adjusting pH to a range of 5.5 to 6.5.
3. The cosmetic formulation as claimed in claim 1, wherein the solvent including Aqua added based on volume of formulation required.
4. The cosmetic formulation as claimed in claim 1, wherein the chelating agent including Disodium EDTA with the predefined quantity in a range of 0.1% to 0.2% by weight of the formulation.
5. The cosmetic formulation as claimed in claim 1, wherein the humectants including Glycerin with the predefined quantity in a range of 2.5% to 3.5% by weight of the formulation and Propylene Glycol with the predefined quantity in a range of 1.5% to 2.5% by weight of the formulation.
6. The cosmetic formulation as claimed in claim 1, wherein the preservative including Sodium Benzoate with the predefined quantity in a range of 0.25% to 0.75% by weight of the formulation.
7. The cosmetic formulation as claimed in claim 1, wherein the solubilizer including PEG 40-hydrogenated castor oil with the predefined quantity in a range of 0.5% to 1.5 % by weight of the formulation.
8. The cosmetic formulation as claimed in claim 1, wherein the fragrant substance including rose fragrance with the predefined quantity in a range of 0.25% to 0.75 % by weight of the formulation.
9. The cosmetic formulation as claimed in claim 1, wherein the pH adjuster including 18% Sodium Hydroxide with the predefined quantity in a range of 0.25% to 0.75% by weight of the formulation.
10. The cosmetic formulation as claimed in claim 1, wherein the predefined quantity of the filtrate in a range of 4.5% to 5.5% by weight of the formulation.
11. The cosmetic formulation as claimed in claim 1, wherein the fermenting including solid state fermentation of the carrot root in Bacillus ginsengisoli.
12. A method for producing a cosmetic formulation for enhancing mositurization, comprising the steps of:
fermenting (101) carrot root using a bacillus culture for obtaining a filtrate;
adding (102) a solvent in a first vessel and a pre-defined quantity of a chelating agent in the first vessel;
stirring (103) the solvent and the chelating agent for obtaining a first mixture;
adding (104) a pre-defined quantity of a plurality of humectants to the first mixture and stirring for obtaining a second mixture;
adding (105) the filtrate to the second mixture while continuously stirring for obtaining a first phase mixture;
mixing (106) a predefined quantity of a solubilizer and a fragrant substance in a second vessel for obtaining a second phase mixture and adding the second phase mixture to the first phase mixture while continuously stirring for obtaining a formulation; and
adding (107) a pH adjuster for adjusting pH of the formulation, thereby producing the cosmetic formulation for enhancing moisture content on skin.
, Description:A COSMETIC FORMULATION AND A METHOD FOR PRODUCING THEREOF
FIELD
[0001] The embodiment herein generally relates to a cosmetic formulation for skin. More specifically, the disclosure relates to a cosmetic formulation for skin obtained from the natural source (bio-ferment).
BACKGROUND AND PRIOR ART
[0002] Skin damage is most common term that persists in personal care and beauty industry. These skin damages are caused due to various reasons. Skin as it ages it loses its moisture content and elasticity which over a period of time leads to skin damage.
[0003] Excess of exposure to the sun, aging, cold weather, pollution, harsh chemicals, stress, beautifying products, acne, rashes leads to skin damages. The type of damages caused differs with different persons.
[0004] Any type of damages caused to the skin cells leads to the release of the free radicals. These free radicals are responsible for the breakdown of the skin collagen resulting the premature aging, dry & dull skin.
[0005] When a person uses cosmetics, their skin absorbs chemicals, which can then enter the bloodstream. People might also inhale powders or ingest some cosmetics. Certain chemicals present in makeup and other cosmetic products can contain ingredients that researchers have linked to serious health concerns. Some of these health concerns include cancer, endocrine disorders, which affect the production of hormones in the body developmental delays neurological problems.
[0006] People can avoid exposure to the potentially harmful chemicals in makeup by reducing their use or switching to safer, nontoxic alternatives.
[0007] Fermentation has been used in food for thousands of years. For example, yeast is used to ferment sugar in grapes to make wine, and bacteria are used to produce vinegar. In India too, we find in our mythological treatises about its application. Food like Idli and Dosa are prepared using fermentation. It’s also an effective method to preserve food, which were very useful centuries ago, before the first fridges!
[0008] Recently, fermented foods such as kombucha, kimchi, and kefir, became popular again thanks to the public awareness of the importance of intestinal flora for general well-being and health. The virtues of fermentation within the human body are therefore well known. At the moment, the interest in fermentation has also started to reach skincare and cosmetics. The cosmetics industry is always looking for innovation, and fermentation products, will likely become the world trend of tomorrow.
[0009] Therefore, there is a need for an improved cosmetic formulation using fermentation. Further, there is a need for a method for producing a cosmetic formulation using fermentation for enhancing moisture content on skin.
OBJECTS
[00010] Some of the objects of the present disclosure are described herein below:
[00011] A main object of the present disclosure is to provide a cosmetic formulation.
[00012] Another object of the present disclosure is to provide a method for producing a cosmetic formulation enhancing moisture content on skin.
[00013] Yet another object of the present disclosure is to provide a method for producing a cosmetic formulation using fermentation exhibiting high antioxidant activity.
[00014] Still another object of the present disclosure is to provide a method for producing a cosmetic formulation for delaying aging and reducing free radical scavenging activities on the skin.
[00015] The other objects and advantages of the present disclosure will be apparent from the following description when read in conjunction with the accompanying drawings, which are incorporated for illustration of preferred embodiments of the present disclosure and are not intended to limit the scope thereof.
SUMMARY
[00016] In view of the foregoing, an embodiment herein provides a cosmetic formulation and method for producing the cosmetic formulation.
[00017] In accordance with an embodiment, the cosmetic formulation for enhancing moisturization, comprises a solvent, a predefined quantity of a chelating agent, a predefined quantity of a plurality of humectants, a predefined quantity of a solubilizer, a predefined quantity of a fragrant substance and a filtrate obtained by fermenting carrot root using a bacillus culture, for enhancing the moisturization. A first mixture is obtained by stirring the solvent and the chelating agent and a second mixture is obtained by adding the humectants to the first mixture while continuously stirring. A first phase mixture is obtained by adding a predefined quantity of the filtrate to the second mixture while continuously stirring and a second phase mixture obtained by mixing the solubilizer and the fragrant substance while continuously stirring. The cosmetic formulation is obtained by adding the second phase mixture to the first phase mixture.
[00018] In accordance with an embodiment, the method comprises the steps of fermenting carrot root using a bacillus culture for obtaining a filtrate, next adding a solvent in a first vessel and a pre-defined quantity of a chelating agent in the first vessel and stirring the solvent and the chelating agent for obtaining a first mixture. Next, a pre-defined quantity of a plurality of humectants is added to the first mixture and stirring for obtaining a second mixture and the filtrate is added to the second mixture while continuously stirring for obtaining a first phase mixture. A predefined quantity of a solubilizer and a fragrant is added in a second vessel for obtaining a second phase mixture and the second phase mixture is added to the first phase mixture while continuously stirring for obtaining a formulation. A pH adjuster is added for adjusting pH of the formulation, thereby producing the cosmetic formulation for enhancing moisture content on skin.
[00019] In an embodiment, the solvent includes Aqua added based on volume of formulation required. In an embodiment, the chelating agent includes Disodium EDTA with the predefined quantity in a range of 0.1% to 0.2% by weight of the formulation. The humectants include Glycerin with the predefined quantity in a range of 2.5% to 3.5% by weight of the formulation and Propylene Glycol with the predefined quantity in a range of 1.5% to 2.5% by weight of the formulation.
[00020] In an embodiment, the preservative includes Sodium Benzoate with the predefined quantity in a range of 0.25% to 0.75% by weight of the formulation. In an embodiment, the solubilizer includes PEG 40-hydrogenated castor oil with the predefined quantity in a range of 0.5% to 1.5 % by weight of the formulation.
[00021] In an embodiment, the fragrant substance includes rose fragrance with the predefined quantity in a range of 0.25% to 0.75 % by weight of the formulation. In an embodiment, the pH adjuster includes 18% Sodium Hydroxide with the predefined quantity in a range of 0.25% to 0.75% by weight of the formulation. In an embodiment, pH of the formulation is adjusted to a range of 5.5 to 6.5 by the pH adjuster. The fermentation is solid state fermentation of the carrot root in Bacillus ginsengisoli.
[00022] These and other aspects of the embodiments herein will be better appreciated and understood when considered in conjunction with the following description and the accompanying drawings. It should be understood, however, that the following descriptions, while indicating preferred embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.

BRIEF DESCRIPTION OF DRAWINGS
[00023] The detailed description is set forth with reference to the accompanying figures. In the figures, the left-most digit(s) of a reference number identifies the figure in which the reference number first appears. The use of the same reference numbers in different figures indicates similar or identical items.
[00024] Fig.1 illustrates a flow chart of a method for producing a cosmetic formulation, according to an embodiment herein;
[00025] Fig.2 illustrates a graph representing antioxidant activity of non-fermented carrot roots and fermented carrot roots, according to an embodiment herein;
[00026] Fig.3 illustrates a graph representing antioxidant activity of a placebo, formulation including 5% filtrate of the fermented carrot roots and a conventional product including 5% of antioxidant ingredient, according to an embodiment herein; and
[00027] Fig. 4 illustrates a graph representing skin moisture content; according to an embodiment herein.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[00028] The embodiments herein and the various features and advantageous details thereof are explained more fully with reference to the non-limiting embodiments and detailed in the following description. Descriptions of well-known components and processing techniques are omitted so as to not unnecessarily obscure the embodiments herein. The examples used herein are intended merely to facilitate an understanding of ways in which the embodiments herein may be practiced and to further enable those of skill in the art to practice the embodiments herein. Accordingly, the examples should not be construed as limiting the scope of the embodiments herein.
[00029] As mentioned above, there is a need for a cosmetic formulation using a ferment filtrate. In particular, there is a need for producing a formulation exhibiting high antioxidant activity and enhancing moisture content on the skin. The embodiments achieve this by providing “A method for producing a cosmetic formulation”. Referring now to the drawings, and more particularly to FIGS. 1 through 4, where similar reference characters denote corresponding features consistently throughout the figures, there are shown preferred embodiments.
[00030] In an embodiment, a cosmetic formulation for enhancing moisturization, comprises a solvent, a chelating agent, a plurality of humectants, a solubilizer, a fragrant substance.
[00031] In an embodiment, a first mixture is obtained by stirring the solvent and the chelating agent. In an embodiment, the solvent includes but not limited to Aqua. In an embodiment, the chelating agent includes but not limited to Disodium EDTA. The pre-defined quantity of the chelating agent includes a weight in a range of 0.1% to 0.2% by weight of formulation, preferably 0.15%. Quantity of the solvent is Q.S to total volume.
[00032] A second mixture obtained by adding the humectants to the first mixture while continuously stirring. The humectants include but not limited to Glycerin and Propylene Glycol. The pre-defined quantity of Glycerin including a range of 2.5% to 3.5% by weight of formulation, preferably 3%. The pre-defined quantity of Propylene Glycol including a range of 1.5% to 2.5% by weight of formulation, preferably 2%.
[00033] In an embodiment, a filtrate is obtained by fermenting carrot root using a bacillus culture, for enhancing the moisturization. The carrot root is fermented by solid state fermentation in Bacillus ginsengisoli. A first phase mixture is obtained by adding a predefined quantity of the filtrate to the second mixture while continuously stirring. The predefined quantity of the filtrate including a range of 4.5% to 5.5% by weight of formulation, preferably 5%.
[00034] A second phase mixture obtained by mixing the solubilizer and the fragrant substance while continuously stirring. In an embodiment, the solubilizer includes but not limited to PEG 40- hydrogenated castor oil. The predefined quantity of the solubilizer including a range of 0.5% to 1.5% by weight of formulation, preferably 1%. In an embodiment, the fragrant substance includes but not limited to rose fragrance. The predefined quantity of the solubilizer including a range of 0.25% to 0.75% by weight of formulation, preferably 0.5%.
[00035] In an embodiment, the cosmetic formulation is obtained by adding the second phase mixture to the first phase mixture. A pH adjuster is added to the cosmetic formulation for adjusting pH to a range of 5.5 to 6.5. In an embodiment, the pH adjuster includes but not limited to 18% Sodium Hydroxide (NaOH).
[00036] Fig.1 illustrates a flow chart of a method for producing a cosmetic formulation, according to an embodiment. In an embodiment, the method includes the steps of fermenting 101 carrot root using a bacillus culture for obtaining a filtrate. The fermentation is solid state fermentation of carrot roots in Bacillus ginsengisoli. Introduction of Bacillus ginsengisoli into rich matrix of carrot roots, mobilizes hard core bio-actives into the solution which is stabilized into a ready to use Active Ingredient. The filtrate is water soluble and effective over a pH range of 4 to 8.
[00037] Next, a solvent is added 102 in a first vessel and a pre-defined quantity of a chelating agent in the first vessel. The solvent and the chelating agent are stirred 103 for obtaining a first mixture.
[00038] Next, adding 104 a pre-defined quantity of a plurality of humectants to the first mixture and stirring the humectants with the first mixture for obtaining a second mixture.
[00039] Next, adding 105 a predefined quantity of the filtrate to the second mixture while continuously stirring for obtaining a first phase mixture.
[00040] Then, mixing 106 a predefined quantity of a solubilizer and a fragrant substance in a second vessel for obtaining a second phase mixture. The second phase mixture is added to the first phase mixture and mixed while continuously stirring using a stirrer for obtaining a formulation.
[00041] Next, a pH adjuster is added 107 for adjusting pH of the formulation. In an embodiment, the pH adjuster includes but not limited to 18% Sodium Hydroxide (NaOH).
[00042] In a preferred embodiment, the method for producing the cosmetic formulation includes providing the first vessel of 1000ml capacity. Purified water is weighed and 880.3 grams of the purified water is added in the first vessel. Next, 1.5 grams of Disodium EDTA and 5 grams of Sodium Benzoate powder are added to the purified water and mixed well for forming the first mixture. Then, 30 grams of Glycerin and 20 grams of Propylene Glycol are added to the first mixture and stirred for obtaining the second mixture. The filtrate is obtained by fermenting carrot root in Bacillus ginsengisoli. 50 grams of the filtrate is added to the second mixture while continuously stirring for obtaining the first phase mixture. 10 grams of PEG-40 hydrogenated castor oil and 5 grams of rose fragrance is added to the first phase mixture while continuously stirring for obtaining the formulation. Finally, 18% Sodium Hydroxide solution is added to the formulation for adjusting pH to a range in between 5.5 to 6.5. The formulation is stored in an air tight container at 25°C.
[00043] Fig.2 illustrates a graph representing antioxidant activity of non-fermented carrot roots and fermented carrot roots. The graph shows Radical Scavenging Activity in % of carrot without fermentation and % and Radical Scavenging Activity in % of carrot with fermentation.
[00044] . Non-fermented carrot roots exhibit around 75% Radical Scavenging Activity corresponding to the antioxidants. Fermented carrot roots exhibit around 120% Radical Scavenging Activity corresponding to higher amount of antioxidants. Fermented carrot roots include potent antioxidants. During skin damage, skin cells release free radicals responsible for breakdown of skin collagen and resulting in premature aging, dryness and dullness of skin. The high antioxidant activity exhibited by the fermented carrot roots attack the released free radicals thereby improving skin dullness and reducing aging. The antioxidants exhibit tyrosinase inhibiting activity providing radiance and glow to the skin.
[00045] Fig. 3 illustrates a graph representing antioxidant activity of a placebo, formulation including 5% filtrate of the fermented carrot roots and a conventional product including 5% of antioxidant ingredient. FRAP (Ferric Reducing Antioxidant Power) assay is used for measuring antioxidants (or reductants) in a provided sample. On interacting with the FRAP assay, the antioxidants present in the sample reduce ferric ion- TPTZ (2,4,6-tri(2-pyridyl)-1,3,5-triazine), thereby formed Fe+2-ligand producing intense blue color. This blue colour is correlated with the antioxidant content of the sample.
[00046] Procedure: One gram of the formulation was extracted with 9 ml of 50% methanol, centrifuged and filtered to obtain a clear extract. Antioxidant activity of the formulation was determined using the FRAP assay protocol. The formulation including 5% of the filtrate of fermented carrot root, a placebo and a conventional product including 5% of its antioxidant ingredient were compared and represented in the graph alongside percentage of exhibited Radical Scavenging Activity (RSA). Clearly, the formulation with 5% of the filtrate of fermented carrot root as sole antioxidant active ingredient provides higher antioxidant activity when compared to the conventional product.
[00047] Fig.4 illustrates a graph representing skin moisture content. An in-vivo study was conducted for 8 hours for evaluating moisturization (hydration capability) of the formulation.
[00048] A multi skin test center MC 750 Corneometer was used as an equipment for measuring moisture content of skin. The equipment includes a moisture module for providing information about moisture content present in the skin by measuring conducting properties of the upper skin layers. Presence of moisture in the skin improves conductance thereby resulting in higher readings than dry skin with minimal amount of moisture. Higher levels of moisture content in the skin results in higher Corneometer readings.
[00049] 10 volunteers (Male/Female) between ages of 25 and 50 and known to be free of any skin pathologies participated. MC 750 Corneometer was used to measure moisture content on the subject’s (volunteer’s) volar forearms. Baseline moisturization readings were taken on day one of the study.
[00050] 50mg of test materials including a base liquid of the formulation without the filtrate of the fermented carrot root, the formulation with 5% of the filtrate of fermented carrot root, and a conventional product with 5% of antioxidant ingredient were applied on volar forearms in 4×4cm. Readings was taken immediately after application of the test materials, after 2 hours, after 8 hours and after 24 hours. Measurements of an untreated test site on the skin was also recorded.
[00051] The table.1 below shows Percentage (%) change of moisture content present on the skin.
Percentage change (%) T=0 T=2 hrs T=8 hrs T=24hrs
Untreated site 30 % 30 % 30 % 30 %
Base liquid 32 % 33 % 40 % 40 %
Formulation with 5% filtrate of fermented carrot root 35 % 40 % 45 % 50 %
Conventional product with 5% antioxidant ingredient 34 % 39 % 40 % 40 %
Table .1
[00052] Each value of the moisture content is an average of three consecutive readings per test site. The graph 400 shows a representation of the above values of change in moisture content on the skin.
[00053] Clearly based on the 8 hours efficacy study, using the formulation with 5% concentration of filtrate of fermented carrot root increased moisture content of the skin by 45%, when compared to 30% moisture content of untreated site, 40% moisture content of conventional product with 5% antioxidant ingredient and 40% moisture content of the base liquid of formulation without filtrate of fermented carrot root. Therefore, the formulation provides higher moisture content to the skin when compared to untreated site, base liquid and conventional product.
[00054] The formulation maintained moisturization at 50% after 24hours whereas the conventional product maintained at 40% and base liquid of the formulation maintained at 40%.
[00055] Therefore, the result confirms that a cosmetic formulation with 5.0% concentration of the filtrate enhances the skin moisture content.
[00056] An advantage of the present disclosure is that the method produces a cosmetic formulation for improving moisturization of skin.
[00057] Another advantage of the present disclosure is that the method produces a cosmetic formulation for revitalizing and toning the skin
[00058] Yet another advantage of the present disclosure is that the method uses bio-ferment filtrates which are water soluble and easier to incorporate in wide range of formulations for skin and hair.
[00059] Still another advantage of the present disclosure is that the method produces a cosmetic formulation rich in antioxidants, thereby reducing free radical scavenging activities, and delaying the aging of the skin.
[00060] Yet another advantage of the present disclosure is that the method produces a cosmetic formulation using bio-ferment having natural vitamins for improving texture of skin and hair.
[00061] Still another advantage of the present disclosure is that the method produces a cosmetic formulation rich in minerals for maintaining texture, elasticity, firmness of the skin, revitalizing the skin and balancing cell activity.
[00062] Yet another advantage of the present disclosure is that the method produces a cosmetic formulation for reducing photo damage, inflammation, oxidative stress and breakdown of extra cellular matrix of the skin.
[00063] Still another advantage of the present disclosure is that the method produces a cosmetic formulation for preventing wrinkles, repairs skin tissues, and protects skin against sun rays.
[00064] Yet another advantage of the present disclosure is that the method produces a cosmetic formulation for aiding collagen production and maintaining skin elasticity.
[00065] Still another advantage of the present disclosure is that the method produces a cosmetic formulation rich in antioxidants for purifying acne prone skin, helping to rebuild the skin barrier of the sensitized skin and protect the skin from pollution.
[00066] The foregoing description of the specific embodiments will so fully reveal the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments herein have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein.