FIELD OF INVENTION
[001] The present disclosure is directed to a health care formulation. Particularly, it relates to a disinfectant formulation with constituents in specific proportions to provide both cleaning and disinfectant activity along with formation of ouzo on dilution. The present disclosure is further directed to a process of preparation of said disinfectant formulation.
BACKGROUND OF THE INVENTION
[002] Almost every environment on earth accommodates a plethora of microorganisms. Some of these microorganisms, specifically termed as pathogens, cause diseases and are harmful for us. In order to prevent such pathogens from causing diseases, several chemical agents called biocides with antimicrobial properties are being used. The biocides can be divided into two categories, namely antiseptics and disinfectants, based upon their use or application. An antiseptic is a chemical agent that is used to inactivate the microbes in or on living tissues, whereas a disinfectant is used to inhibit microbial growth on inanimate objects or nonliving surfaces.
[003] Disinfectants have been used widely for sterilizing domestic and commercial spaces. An example would be health care facilities, such as hospitals. Disinfectants are used for topical and other applications in hospitals in order to prevent the spread of hospital-acquired or nosocomial infections. The use of disinfectants among the public has also increased in recent times because of the increased consumer awareness and a sharp rise in infection risks. Similar to disinfectants, antiseptics also have an important role to play in sterilization of living tissue/skin. They are generally antimicrobial substances that are applied to reduce the risk of infection, sepsis and putrefaction. Antiseptics are extensively been used in health care facilities and for personal use as well.
[004] The chemical agents employed in making a disinfectant or an antiseptic formulation with antimicrobial activity can be broadly classified into seven categories. They are chlorine containing compounds, alcohols, aldehydes, anilides, iodophores, quaternary ammonium compounds and phenolic compounds. The chlorine containing compounds such as bleaches are widely known to be corrosive and cause discoloration, alcohols evaporate rapidly and are not long-lasting, and iodophores have an unpleasant smell and can stain the surfaces on which they are used. Phenolic compounds have been extensively used as disinfectants and have been very effective against the pathogens. However, these phenolic compounds suffer from drawbacks of toxicity and have problems associated with regards to their safe disposal.

The quaternary ammonium compounds (QACs) make for a good choice as disinfectants and antiseptics as these compounds show broad spectrum antimicrobial activity. Additionally, the QACs are cationic surfactants and therefore can provide both disinfecting and cleaning properties with minimal side effects.
[005] There are a variety of antiseptics and disinfectants available in the market with predominant market share lying with phenolic compounds. These formulations containing phenolic compounds need to be diluted before use and since they contain castor oil as one of the constituents, one can observe an ouzo effect upon dilution. Ouzo effect refers to the formation of turbid (milky) dispersion of a solution on dilution in water. The phenolic disinfectant formulations have been extensively used by the consumers since decades, therefore, there is a common perception among the public that the effectiveness of a disinfectant solution is associated with the formation of cloudiness (ouzo) upon dilution. Hence the formation of ouzo upon dilution has an important aesthetic appeal among disinfectant formulations.
[006] As discussed, the main problems associated with the existing disinfectant products are corrosiveness, ephemeral nature and staining characteristics. Therefore, there is need for an efficient disinfectant formulation which is capable of overcoming these problems associated with the existing disinfectant products; along with a satisfactory formation of ouzo effect as a key aesthetic feature.
SUMMARY OF THE INVENTION
[007] In an aspect of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene and derivatives thereof; and (c) at least one solvent; wherein the disinfectant formulation forms a turbid (milky) dispersion on dilution with water.
[008] In an aspect of the present disclosure, there is provided a process for the preparation of disinfectant formulation comprising: (a) at least one antimicrobial agent; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene and derivatives thereof; and (c) at least one solvent; wherein the disinfectant formulation forms a milky dispersion on dilution with water, said process comprising the steps of (a) obtaining at least one antimicrobial agent; (b) obtaining at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene and derivatives thereof; (c) obtaining at least one solvent; and (d)

mixing the at least one antimicrobial agent and the at least one ingredient selected from the
group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene and
derivatives thereof, and at least one solvent to obtain the disinfectant formulation.
[009] These and other features, aspects, and advantages of the present subject matter will be
better understood with reference to the following description and appended claims. This
summary is provided to introduce a selection of concepts in a simplified form. This summary
is not intended to identify key features or essential features of the claimed subject matter, nor
is it intended to be used to limit the scope of the claimed subject matter.
BRIEF DESCRIPTION OF DRAWINGS
[0010] Figure 1 depicts the individual value plot data for the globule size calculated from
confocal microscopic experiments.
[0011] Figure 2a depicts the confocal microscopic image (at 40x magnification) of the
disinfectant formulation as described in the present invention, prior to dilution.
[0012] Figure 2b depicts the confocal microscopic image (at 40x magnification) of the
disinfectant formulation as described in the present invention, after dilution with water.
DETAILED DESCRIPTION OF THE INVENTION
[0013] Those skilled in the art will be aware that the present disclosure is subject to
variations and modifications other than those specifically described. It is to be understood
that the present disclosure includes all such variations and modifications. The disclosure also
includes all such steps, features, compositions, and compounds referred to or indicated in this
specification, individually or collectively, and any and all combinations of any or more of
such steps or features.
Definitions
[0014] For convenience, before further description of the present disclosure, certain terms
employed in the specification, and examples are collected here. These definitions should be
read in the light of the remainder of the disclosure and understood as by a person of skill in
the art. The terms used herein have the meanings recognized and known to those of skill in
the art, however, for convenience and completeness, particular terms and their meanings are
set forth below.
[0015] The articles "a", "an" and "the" are used to refer to one or to more than one (i.e., to at
least one) of the grammatical object of the article.

[0016] The terms "comprise" and "comprising" are used in the inclusive, open sense,
meaning that additional elements may be included. It is not intended to be construed as
"consists of only".
[0017] Throughout this specification, unless the context requires otherwise the word
"comprise", and variations such as "comprises" and "comprising", will be understood to
imply the inclusion of a stated element or step or group of element or steps but not the
exclusion of any other element or step or group of element or steps.
[0018] The term "including" is used to mean "including but not limited to". "Including" and
"including but not limited to" are used interchangeably.
[0019] The term "at least one" is used to mean one or more and thus includes individual
components as well as mixtures/combinations.
[0020] The term "disinfectant" used hereinafter throughout the disclosure relates to both
"disinfectant" and "antiseptic" formulations collectively. The terms may be used
interchangeably.
[0021] Ratios, concentrations, amounts, and other numerical data may be presented herein in
a range format. It is to be understood that such range format is used merely for convenience
and brevity and should be interpreted flexibly to include not only the numerical values
explicitly recited as the limits of the range, but also to include all the individual numerical
values or sub-ranges encompassed within that range as if each numerical value and sub-range
o
is explicitly recited. For example, a temperature ranges of about 25-40 C should be
o o
interpreted to include not only the explicitly recited limits of about 25 C to about 40 C, but
o o
also to include sub-ranges, such as 25-30 C, 28-38 C, and so forth, as well as individual
o
amounts, including fractional amounts, within the specified ranges, such as 25.2 C, and 38.5
o
C, for example.
[0022] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference. [0023] The present disclosure is not to be limited in scope by the specific implementations described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein.

[0024] The main problems associated with the existing disinfectant products are their abrasiveness, transient nature, awful smell and staining properties. These problems have been associated with different categories of disinfectants as already discussed. Therefore, there is a need for an antimicrobial disinfectant formulation which is non-corrosive, long-lasting, pleasant smelling and non-staining on the surfaces on which it is used. Along with the above-mentioned properties, the disinfectant formulation should also be capable of forming a milky dispersion on dilution with water.
[0025] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) at least one solvent, wherein the disinfectant formulation forms a milky dispersion on dilution with water.
[0026] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600.
[0027] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) at least one solvent, wherein the disinfectant formulation forms a milky dispersion on dilution with water and the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600. [0028] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one antimicrobial agent is selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof. [0029] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one solvent is selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof, wherein the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1:100. [0030] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one

ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) at least one solvent selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on dilution with water and the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 -1:100.
[0031] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof, having weight percentage in the range of 4 - 60% with respect to the formulation; and (c) at least one solvent having weight percentage in the range of 20 - 70% with respect to the formulation.
[0032] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof, having weight percentage in the range of 4 - 60% with respect to the formulation; and (c) at least one solvent having weight percentage in the range of 20 - 70% with respect to the formulation, wherein the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1:100. [0033] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60% with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) at least one solvent having weight percentage in the range of 20 -70%) with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on

dilution with water and the at least one antibacterial agent to the at least one ingredient
weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at
least one solvent weight ratio is in the range of 1:0.01 - 1:100.
[0034] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, further comprising at least one excipient selected from: (a)
fragrance having weight percentage in the range of 0.1 - 20% with respect to the formulation
and selected from the group consisting of capolene, terpineol, and combinations thereof; (b)
colorant having weight percentage in the range of 0.001 - 0.5% with respect to the
formulation and selected from the group consisting of tartrazine, sunset yellow, brilliant blue,
direct blue, and combinations thereof; and (c) at least one pH adjuster having weight
percentage as much as is required to maintain the pH of the formulation in the range of 6.5 -
7.0 and selected from the group consisting of sodium hydroxide, D gluconolactone, and
combinations thereof.
[0035] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the fragrance has a weight percentage in the range
of 0.1 - 20%) with respect to the formulation and is selected from the group consisting of
capolene, terpineol, and combinations thereof.
[0036] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the fragrance is selected from the group consisting
of capolene, terpineol, and combinations thereof.
[0037] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the fragrance is capolene. In another embodiment of
the present disclosure, the fragrance is terpineol. In yet another embodiment of the present
disclosure, the fragrance is a combination of capolene and terpineol.
[0038] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the colorant has a weight percentage in the range of
0.001 - 0.5%) with respect to the formulation and is selected from the group consisting of
tartrazine, sunset yellow, brilliant blue, direct blue, and combinations thereof.
[0039] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the colorant has a weight percentage in the range of
0.001 - 0.5%) with respect to the formulation.
[0040] In an embodiment of the present disclosure, there is provided a disinfectant
formulation as described herein, wherein the colorant is selected from the group consisting of
tartrazine, sunset yellow, brilliant blue, direct blue, and combinations thereof. In another
o

embodiment of the present disclosure, the colorant is a combination of tartrazine and sunset yellow.
[0041] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one pH adjuster has a weight percentage as much as is required to maintain the pH of the formulation in the range of 6.5 - 7.0 and is selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof.
[0042] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one pH adjuster has a weight percentage as much as is required to maintain the pH of the formulation in the range of 6.5 - 7.0. [0043] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one pH adjuster is selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof. [0044] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the at least one pH adjuster is sodium hydroxide. In another embodiment of the present disclosure, the at least one pH adjuster is a combination of sodium hydroxide and D gluconolactone.
[0045] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60% with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; (c) at least one solvent having weight percentage in the range of 20 -70%) with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof; and (d) fragrance having weight percentage in the range of 0.1 -20%) with respect to the formulation and selected from the group consisting of capolene, terpineol, and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on dilution with water and the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1:100.

[0046] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60% with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; (c) at least one solvent having weight percentage in the range of 20 -70%) with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof; and (d) colorant having weight percentage in the range of 0.001 -0.5%) with respect to the formulation and selected from the group consisting of tartrazine, sunset yellow, brilliant blue, direct blue, and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on dilution with water and the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 -1:100.
[0047] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10%> with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60%> with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene, and derivatives thereof; (c) at least one solvent having weight percentage in the range of 20 -70%o with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof; and (d) at least one pH adjuster having weight percentage as much as is required to maintain the pH of the formulation in the range of 6.5 - 7.0 and selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on dilution with waterand the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01- 1:100.

[0048] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60% with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene, and derivatives thereof; (c) at least one solvent having weight percentage in the range of 20 -70%) with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof; (d) fragrance having weight percentage in the range of 0.1 - 20% with respect to the formulation and selected from the group consisting of capolene, terpineol, and combinations thereof; (e) colorant having weight percentage in the range of 0.001 - 0.5% with respect to the formulation and selected from the group consisting of tartrazine, sunset yellow, brilliant blue, direct blue, and combinations thereof; and (f) at least one pH adjuster having weight percentage as much as is required to maintain the pH of the formulation in the range of 6.5 - 7.0 and selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof, wherein the disinfectant formulation forms a milky dispersion on dilution with waterand the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1:100. [0049] In an embodiment of the present disclosure, there is provided a disinfectant formulation as described herein, wherein the formulation further comprises water having weight percentage as much as is required to make the formulation 100%. [0050] In an embodiment of the present disclosure, there is provided a disinfectant formulation comprising: (a) at least one antimicrobial agent having weight percentage in the range of 0.1 - 10% with respect to the formulation and selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride, and combinations thereof; (b) at least one ingredient having weight percentage in the range of 4 - 60% with respect to the formulation and selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene, and derivatives thereof; (c) at least one solvent having weight percentage in the range of 20 -70%) with respect to the formulation and selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms),

and combinations thereof; (d) fragrance having weight percentage in the range of 0.1 - 20% with respect to the formulation and selected from the group consisting of capolene, terpineol, and combinations thereof; (e) colorant having weight percentage in the range of 0.001 - 0.5% with respect to the formulation and selected from the group consisting of tartrazine, sunset yellow, brilliant blue, direct blue, and combinations thereof; (f) at least one pH adjuster having weight percentage as much as is required to maintain the pH of the formulation in the range of 6.5 - 7.0 and selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof; and (g) water having weight percentage as much as is required to make the formulation 100%), wherein the disinfectant formulation forms a milky dispersion on dilution with water and the at least one antibacterial agent to the at least one ingredient weight ratio is in the range of 1:0.4 - 1:600 and the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1:100. [0051] In an embodiment of the present disclosure, there is provided a process for the preparation of disinfectant formulation comprising: (a) at least one antimicrobial agent; (b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) at least one solvent, said process comprising the steps of: (a) obtaining at least one antimicrobial agent; (b) obtaining at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof; and (c) obtaining at least one solvent; and (d) mixing the at least one antimicrobial agent, the at least one ingredient selected from the group comprising polysorbate 80, isopropyl myrisate, terpineol, span 80, capolene, and derivatives thereof, and at least one solvent to obtain the disinfectant formulation, wherein the disinfectant formulation forms a milky dispersion on dilution with water. [0052] In an embodiment of the present disclosure, there is provided a process for the preparation of the disinfectant formulation as described herein, wherein the said process comprising the steps of: (a) mixing at least one solvent and at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, to obtain a first mixture; (b) mixing the first mixture with at least one ingredient selected from terpineol and capolene to obtain a second mixture; (c) mixing the second mixture with at least one antimicrobial agent to obtain a third mixture; (d) mixing at least one colorant with water to obtain a fourth mixture; (e) mixing the third mixture with the fourth mixture to obtain a fifth mixture; (f) mixing the fifth mixture with water to obtain a sixth mixture; (g) mixing the sixth mixture with at least one pH adjuster to obtain a seventh mixture; and (h) mixing the seventh mixture with water to obtain the formulation.

[0053] In an embodiment of the present disclosure, there is provided a process for the preparation of the disinfectant formulation as described herein, wherein the said process comprising the steps of: (a) mixing at least one solvent and at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a first mixture; (b) mixing the first mixture with at least one ingredient selected from terpineol and capolene, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a second mixture; (c) mixing the second mixture with at least one antimicrobial agent followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a third mixture; (d) mixing at least one colorant with water at a temperature in the range of 20 - 35 °C to obtain a fourth mixture; (e) mixing the third mixture with the fourth mixture followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a fifth mixture; (f) mixing the fifth mixture with water at a temperature in the range of 20 - 35 °C to obtain a sixth mixture; (g) mixing the sixth mixture with at least one pH adjuster at a temperature in the range of 20 - 35 °C to obtain a seventh mixture; and (h) mixing the seventh mixture with water at a temperature in the range of 20 - 35 °C to obtain the formulation.
[0054] In an embodiment of the present disclosure, there is provided a process for the preparation of the disinfectant formulation as described herein, wherein the said process comprising the steps of: (a) mixing at least one solvent with at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, terpineol, capolene to obtain a first mixture; (b) mixing the first mixture with at least one antimicrobial agent to obtain a second mixture; (c) mixing at least one colorant with water to obtain a third mixture; (d) mixing the second mixture with the third mixture to obtain a fourth mixture; (e) mixing the fourth mixture with water to obtain a fifth mixture; (f) mixing the fifth mixture with at least one pH adjuster to obtain a sixth mixture; and (g) mixing the sixth mixture with water to obtain the formulation.
[0055] In an embodiment of the present disclosure, there is provided a process for the preparation of the disinfectant formulation as described herein, wherein the said process comprising the steps of: (a) mixing at least one solvent with at least one ingredient selected from the group comprising polysorbate 80, terpineol, capolene isopropyl myristate, span 80,

followed by stirring at a temperature in the range of 20 - 35 °C to obtain a first mixture; (b) mixing the first mixture with at least one antimicrobial agent, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a second mixture; (c) mixing at least one colorant with water at a temperature in the range of 20 - 35 °C to obtain a third mixture; (d) mixing the second mixture with the third mixture, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a fourth mixture; (e) mixing the fourth mixture with water at a temperature in the range of 20 - 35 °C to obtain a fifth mixture; (f) mixing the fifth mixture with at least one pH adjuster at a temperature in the range of 20 - 35 °C to obtain a sixth mixture; and (g) mixing the sixth mixture with water at a temperature in the range of 20 - 35 °C to obtain the formulation. EXAMPLES
[0056] The working examples of the present disclosure would now be illustrated. These examples are neither restrictive nor intended to be limiting to the scope of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
[0057] The present invention describes a disinfectant formulation that renders strong antimicrobial activity along with furnishing properties not provided by existing disinfectant products, such as, being non-toxic, being non-abrasive on the surface applied, having pleasant fragrance and also forming a milky dispersion upon dilution with water. The process of obtaining the formulation and its characterization is discussed below in detail. Example 1
Experiments with Confocal microscopy to test the formation of ouzo [0058] Confocal microscopy (carried out on Laser Scanning Confocal Microscope 710 (CLSM-710) [Make: Carl Zeiss] instrument) was used to study and establish the stability of the ouzo formation upon dilution of the formulation with water. For the purpose, the disinfectant globules and their sizes were studied before and after dilution. The data in Table 1 indicates the dispersion of large sized globules into smaller sized globules upon dilution.

The associated individual value plot of the globule size (in micrometer) is provided in Figure 1. The formation of small sized globules is indicative of the onset of ouzo effect.
[0059] The data shown in figures 2a and 2b, provide contrasting confocal microscopic images of the formulation before dilution (2a) and after dilution (2b). Typical dilution involved a 100-fold addition of water to the disinfectant formulation. The images were obtained at the same magnification (40x) in order to provide accurate comparison of the data. As is clear, the formulation exists as an emulsion prior to dilution (2a) and the emulsion starts disintegrating upon dilution (2b) giving rise to the milky ouzo. Antimicrobial disinfectant formulation providing ouzo effect:
[0060] Table 2 below displays the ingredients used in the disinfectant formulation having antimicrobial activity along with formation of ouzo as described hereinabove in the specification along with the working range of the various ingredients.

[0061] Generally, decontaminant formulations need to be diluted with water before use. The phenolic disinfectant formulations form a milky dispersion when diluted with water (referred to as the ouzo effect). Market research has revealed that consumers relate this formation of milky dispersion to enhanced antimicrobial activity associated with the phenolic disinfectants. Hence, in addition to providing enhanced antimicrobial activity, the formation of ouzo is necessary to obtain consumer acceptance. The examples in Table 2 show various formulations that worked as disinfectant formulations rendering long-lasting broad spectrum

antimicrobial activity without having the drawbacks of unpleasant smell and staining tendency. All the examples illustrated in Table 2 formed a milky dispersion (ouzo) upon dilution with water. All formulations mentioned in Table 2 contained benzalkonium chloride (having weight percentage of 8%) and isopropyl alcohol (having weight percentage of 35%) as constituents, while the weight percentage of other constituents were varied. It was observed that the example El which contained terpineol (8%), polysorbate (8%) and isopropyl myristate (8%) managed to form a stable milky dispersion on dilution with water. The other two examples which also formed stable milky dispersions upon dilution, were E8 and E9. E8 contained terpineol (8%), polysorbate (8%), isopropyl myristate (8%) and capolene (8%) as least one ingredient and E9 constituted capolene (8%), polysorbate (8%) and isopropyl myristate (8%). All other examples, that is, E2, E3, E4, E5, E6, E7, E10, Ell and E12 formed non-prominent or unstable ouzo upon dilution. These examples which formed non-prominent milky dispersion on dilution with water either lacked polysorbate 80 (as in E10 and Ell) or isopropyl myristate (as in E3, E6 and E7) or both (as in E2, E4, E5 and E12). Thus, an inference can be drawn that for the formation of a stable milky dispersion on dilution, both polysorbate 80 and isopropyl myristate should be present in the formulation as ingredients.
[0062] Table 3 below illustrates further examples that were attempted in order to test the compatibility of the antimicrobial agent with commonly used castor oil based disinfectant additives.

sulfate
Observations
Ouzo formation - No ouzo No ouzo No ouzo
* values given in weight percentages
[0063] Traditional ouzo forming formulations have employed a combination of castor oil with phenols. The effect of aqueous dilution with such formulations leads to the preferred milky cloudiness. However, as observed from the Table 3, none of the formulations E13-E15 provide an acceptable ouzo formation despite the use of castor oil and castor oil-based soaps. An inference regarding the high aqueous solubility of benzalkonium chloride (a quaternary ammonium salt) may be drawn to explain the observed ineffectiveness of these formulations towards ouzo formation. The importance of additives such as isopropyl myristate and polysorbate 80 can be appreciated on comparison of the performance of samples El, E8 and E9 versus the samples presented in Table 3.
[0064] Table 4 below shows a specific example that fulfilled all the requirements of the disinfectant formulation.
[0065] The formulation as presented in Table 4, contained a 1:1 (weight by weight) mixture of the antimicrobial agent (benzalkonium chloride) to terpineol. The formulation was further stabilized by the presence of isopropyl alcohol. The formulation was found to be effective in formation of milky ouzo though the ouzo so formed was not prominent. Furthermore, the presence of quaternary ammonium surfactant provides the formulation disinfectant as well cleansing properties. Process for preparation of the disinfectant formulation as described in Table 4

[0066] The formulation having the composition as described in Table 4, was prepared by first weighing the desired quantity of isopropyl alcohol into a cleaned vessel equipped with provision for stirring. Terpineol was dissolved into the alcohol under stirring (500 rpm) over a period of 15 min. until clear solution of the first mixture was obtained. Second mixture was obtained by dissolving benzalkonium chloride into the first mixture under stirring (500 rpm) for a period of about 15 min. until clear solution was obtained. The third mixture was obtained by dissolving the colours (tartrazine and sunset yellow) in water under stirring (500 rpm) for about a period of 15 min. until a clear solution was obtained. The aqueous solution of colours was then dissolved into the second mixture to obtain a fourth mixture. The fourth mixture was further diluted with water to make up 90% of the total weight of the formulation to obtain a fifth mixture. The pH of the fifth mixture was adjusted within a range of 6.5-7.0 by adding a 1% solution of sodium hydroxide to obtain a sixth mixture. Finally, water was added to the sixth mixture to make up the total weight percentage to 100% to obtain the disinfectant formulation.
[0067] The formulation as presented in Table 5, contained a 1:1 (weight by weight) mixture of the cationic surfactant (benzalkonium chloride) to terpineol. The addition of isopropyl myristate and polysorbate 80 assists in stabilizing the ouzo formation, as well as enhancing the antimicrobial ability of the disinfectant formulation.

Process for preparation of the disinfectant formulation as described in Table 5 [0068] The formulation having the composition as described in Table 5, was prepared by first weighing the desired quantity of isopropyl alcohol into a cleaned vessel equipped with provision for stirring. Polysorbate 80, isopropyl myrstate and span 80 were added to the vessel and the first mixture was obtained by stirring the mixture at a speed of 500 rpm for about a period of 15 min until clear solution was obtained. Terpineol was dissolved into the first mixture under stirring (500 rpm) over a period of 15 min until clear solution of the second mixture was obtained. Third mixture was obtained by dissolving benzalkonium chloride into the second mixture under stirring (500 rpm) for a period of about 15 min until clear solution was obtained. The fourth mixture was obtained by dissolving the colours (tartrazine and sunset yellow) in water under stirring (500 rpm) for about a period of 15 min until a clear solution was obtained. The aqueous solution of colours was then dissolved into the third mixture to obtain a fifth mixture. The fifth mixture was further diluted with water to make up 90% of the total weight of the formulation to obtain a sixth mixture. The pH of the sixth mixture was adjusted within a range of 6.5-7.0 by adding a 1% solution of sodium hydroxide or D-gluconolactone to obtain a seventh mixture. Finally, water was added to the seventh mixture to make up the total weight percentage to 100% to obtain the disinfectant formulation.

10. Purified water qsto 100%
[0069] The formulation as presented in Table 6, contained a 1:1 (weight by weight) mixture of the cationic surfactant (chlorhexidine gluconate) to terpineol. The addition of isopropyl myristate and polysorbate 80 assists in stabilizing the ouzo formation, as well as enhancing the antimicrobial ability of the disinfectant formulation.
Process for preparation of the disinfectant formulation as described in Table 6 [0070] The formulation having the composition as described in Table 6, was prepared by first weighing the desired quantity of isopropyl alcohol into a cleaned vessel equipped with provision for stirring. Polysorbate 80, isopropyl myristate and span 80 were added to the vessel and the first mixture was obtained by stirring the mixture at a speed of 500 rpm for about a period of 15 min until clear solution was obtained. Terpineol was dissolved into the first mixture under stirring (500 rpm) over a period of 15 min until clear solution of the second mixture was obtained. Third mixture was obtained by dissolving chlorhexidine gluconate into the second mixture under stirring (500 rpm) for a period of about 15 min until clear solution was obtained. The fourth mixture was obtained by dissolving the colours (tartrazine and sunset yellow) in water under stirring (500 rpm) for about a period of 15 min until a clear solution was obtained. The aqueous solution of colours was then dissolved into the third mixture to obtain a fifth mixture. The fifth mixture was further diluted with water to make up 90% of the total weight of the formulation to obtain a sixth mixture. The pH of the sixth mixture was adjusted within a range of 6.5-7.0 by adding a 1% solution of sodium hydroxide or D-gluconolactone to obtain a seventh mixture. Finally, water was added to the seventh mixture to make up the total weight percentage to 100% to obtain the disinfectant formulation.

Sunset Yellow

0.002

Sodium hydroxide/ D gluconolactone (pH adjuster)

qs

10.

Purified water

qstol00%

[0071] The formulation as presented in Table 6, contained a 1:1 (weight by weight) mixture of the cationic surfactant (Cetrimide) to terpineol. The addition of isopropyl myristate and polysorbate 80 assists in stabilizing the ouzo formation, as well as enhancing the antimicrobial ability of the disinfectant formulation.
Process for preparation of the disinfectant formulation as described in Table 7 [0072] The formulation having the composition as described in Table 7, was prepared by first weighing the desired quantity of isopropyl alcohol into a cleaned vessel equipped with provision for stirring. Polysorbate 80, isopropyl myrstate and span 80 were added to the vessel and the first mixture was obtained by stirring the mixture at a speed of 500 rpm for about a period of 15 min until clear solution was obtained. Terpineol was dissolved into the first mixture under stirring (500 rpm) over a period of 15 min until clear solution of the second mixture was obtained. Third mixture was obtained by dissolving cetrimide into the second mixture under stirring (500 rpm) for a period of about 15 min until clear solution was obtained. The fourth mixture was obtained by dissolving the colours (tartrazine and sunset yellow) in water under stirring (500 rpm) for about a period of 15 min until a clear solution was obtained. The aqueous solution of colours was then dissolved into the third mixture to obtain a fifth mixture. The fifth mixture was further diluted with water to make up 90% of the total weight of the formulation to obtain a sixth mixture. The pH of the sixth mixture was adjusted within a range of 6.5-7.0 by adding a 1% solution of sodium hydroxide or D-gluconolactone to obtain a seventh mixture. Finally, water was added to the seventh mixture to make up the total weight percentage to 100% to obtain the disinfectant formulation. [0073] All other examples described hereinabove (Tables 2-3) were prepared using the procedure described in the preceding paragraphs.
[0074] Although the subject matter has been described in considerable detail with reference to certain examples and implementations thereof, other implementations are possible. Advantages gained in the example illustrative process in this subject matter: [0075] The cationic disinfectant-based formulation disclosed herein achieves a milky or turbid dispersion (ouzo) upon dilution with water.

[0076] The present disinfectant formulation is advantageous in terms of having a number of desirable properties such as being microbicidal, non-staining, pleasant smelling and non-corrosive, while providing an acceptable ouzo formation upon dilution with water.

I/We Claim:
1. A disinfectant formulation comprising:
(a) at least one antimicrobial agent;
(b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene and derivatives thereof; and
(c) at least one solvent
wherein the disinfectant formulation forms a milky dispersion on dilution with water.
2. The formulation as claimed in claim 1, wherein the at least one antimicrobial agent to the at least one ingredient weight ratio is in the range of 1: 0.4 - 1: 600.
3. The formulation as claimed in claim 1, wherein the at least one antimicrobial agent is selected from the group consisting of benzalkonium chloride, cetrimide, cetrimide along with chlorhexidine gluconate, cetyl trimethyl ammonium chloride and combinations thereof.
4. The formulation as claimed in claim 1, wherein the at least one solvent is selected from the group consisting of isopropyl alcohol, ethanol, propylene glycol, glycerin, petroleum ether, polyethylene glycol (all forms), and combinations thereof, wherein the at least one antimicrobial agent to the at least one solvent weight ratio is in the range of 1:0.01 - 1: 100.
5. The disinfectant formulation as claimed in any of the claims 1-4, comprising:

(a) at least one antimicrobial agent having weight percentage in the range of 0.1 -10% with respect to the formulation;
(b) at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene and derivatives thereof, having weight percentage in the range of 4 - 60% with respect to the formulation.
(c) at least one solvent having weight percentage in the range of 20 - 70% with respect to the formulation.
6. The formulation as claimed in claim the 5, further comprising at least one excipient
selected from: (a) fragrance having weight percentage in the range of 0.1 - 20% with
respect to the formulation and selected from the group consisting of capolene, terpineol
and combinations thereof; (b) colorant having weight percentage in the range of 0.001 -
0.5%) with respect to the formulation and selected from the group consisting of
tartrazine, sunset yellow and combinations thereof; (c) at least one pH adjuster having
weight percentage as much as is required to maintain the pH of the formulation in the

range of 6.5 - 7.0 and selected from the group consisting of sodium hydroxide, D gluconolactone, and combinations thereof.
7. The formulation as claimed in any of claims 5-6, further comprising water having weight percentage as much as is required to make the formulation 100%.
8. A process for the preparation of formulation as claimed in claim 1, comprising the steps of: (a) obtaining at least one antimicrobial agent; (b) obtaining at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene and derivatives thereof; (c) obtaining at least one solvent; and (d) mixing the at least one antimicrobial agent, the at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, terpineol, span 80, capolene and derivatives thereof, and at least one solvent to obtain the formulation.
9. A process for the preparation of formulation as claimed in any of the claims 1-7, comprising the steps of: (a) mixing at least one solvent and at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, to obtain a first mixture; (b) mixing the first mixture with at least one ingredient selected from terpineol and capolene to obtain a second mixture; (c) mixing the second mixture with at least one antimicrobial agent to obtain a third mixture; (d) mixing at least one colorant with water to obtain a fourth mixture; (e) mixing the third mixture with the fourth mixture to obtain a fifth mixture; (f) mixing the fifth mixture with water to obtain a sixth mixture; (g) mixing the sixth mixture with at least one pH adjuster to obtain a seventh mixture; and (h) mixing the seventh mixture with water to obtain the formulation.
10. The process as claimed in claim 9, comprising the steps of: (a) mixing at least one solvent and at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, followed by stirring at a temperature in the range of 20 -35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 -700 rpm to obtain a first mixture; (b) mixing the first mixture with at least one ingredient selected from terpineol and capolene, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a second mixture; (c) mixing the second mixture with at least one antimicrobial agent followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a third mixture; (d) mixing at least one colorant with water at a temperature in the range of 20 - 35 °C to obtain a fourth mixture; (e) mixing the third

mixture with the fourth mixture followed by stirring at a temperature in the range of 20 -35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 -700 rpm to obtain a fifth mixture; (f) mixing the fifth mixture with water at a temperature in the range of 20 - 35 °C to obtain a sixth mixture; (g) mixing the sixth mixture with at least one pH adjuster at a temperature in the range of 20 - 35 °Cto obtain a seventh mixture; and (h) mixing the seventh mixture with water at a temperature in the range of 20 - 35 °Cto obtain the formulation.
11. A process for the preparation of formulation as claimed in any of the claims 1-7, comprising the steps of: (a) mixing at least one solvent with at least one ingredient selected from the group comprising polysorbate 80, isopropyl myristate, span 80, terpineol, capolene to obtain a first mixture; (b) mixing the first mixture with at least one antimicrobial agent to obtain a second mixture; (c) mixing at least one colorant with water to obtain a third mixture; (d) mixing the second mixture with the third mixture to obtain a fourth mixture; (e) mixing the fourth mixture with water to obtain a fifth mixture; (f) mixing the fifth mixture with at least one pH adjuster to obtain a sixth mixture; and (g) mixing the sixth mixture with water to obtain the formulation.
12. The process as claimed in claim 11, comprising the steps of: (a) mixing at least one solvent with at least one ingredient selected from the group comprising polysorbate 80, terpineol, capolene isopropyl myristate, span 80, followed by stirring at a temperature in the range of 20 - 35 °Cto obtain a first mixture; (b) mixing the first mixture with at least one antimicrobial agent, followed by stirring at a temperature in the range of 20 - 35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 - 700 rpm to obtain a second mixture; (c) mixing at least one colorant with water at a temperature in the range of 20 - 35 °C to obtain a third mixture; (d) mixing the second mixture with the third mixture, followed by stirring at a temperature in the range of 20 -35 °C for a period in the range of 10 - 30 minutes at a stirring speed in the range of 300 -700 rpm to obtain a fourth mixture; (e) mixing the fourth mixture with water at a temperature in the range of 20 - 35 °C to obtain a fifth mixture; (f) mixing the fifth mixture with at least one pH adjuster at a temperature in the range of 20 - 35 °Cto obtain

a sixth mixture; and (g) mixing the sixth mixture with water at a temperature in the range of 20 - 35°C to obtain the formulation.