FIELD OF THE INVENTION:
The present invention relates generally to the field of disinfectant(s) useful for surface wash
and sterilization of worm eggs. More particularly the invention relates to a disinfectant(s) for
cleaning and surface sterilization of Tasar silkworm, Antheraea mylitta Drury eggs.
The Invention further extends to provide a process for surface sterilization of oak tasar
silkworm worm eggs by utilizing said disinfectant.
BACKGROUND AND PRIOR ART:
Diseases accounts for more than 40% loss of silk cocoon yield and thus pose big problem in
tasar culture. In severe infection some times complete failure of cocoon crop was also
noticed. All diseases of tasar silkworm mainly transmit through the trans ovum. Therefore,
the surface of the tasar silkworm eggs should be cleaned and disinfected properly to avoid
transmission of various diseases through the trans ovum transmission.
Various kinds of diseases are serious threat to tasar silkworm, Antheraea mylitta D. It is a
wild insect and reared in outdoor for production of tasar silk cocoons. Disease damage is a
serious problem in tasar culture and loss due to diseases is about 40%. All diseases of tasar
silkworm mainly transmit through the trans ovum therefore, the surface of the tasar silkworm
eggs should be cleaned and disinfected properly to avoid transmission of various diseases.
The conventionally existing process utilizes multiple steps and different chemical(s) in
washing silkworm eggs, like sodium hydroxide solution, hydrochloric acid, formaldehyde
solution etc. The process is multi stepped, lengthy, cumbersome, more time taking,
moderately effective, demands more chemicals and physical apparatus, includes consumption
of high man power, with moderate cocoon yield. Now a days, the cleaning and sterilization of
egg surface using hazardous and band chemicals with cumbersome process is being
practiced.
At present, surface cleaning and sterilization of tasar silkworm eggs by the eco-user friendly
and single step methods are not available.
Following drawbacks are found in the currently existing processes being used for sterilization
of silkworm eggs:

1. Chemicals involved in the existing processes are not eco-user friendly and
emits pungent smell and therefore causes disease in users.
2. Most of the processes are lengthy, cumbersome process and takes about 1 hour
for cleaning the egg surface.
3. The conventional processes have multiple requirements for egg washing like,
physical apparatus i.e. multiple container(s), measuring jar(s), nylon net along
with different chemicals and large quantity of water.
4. The disinfectant solution is not a ready-made for immediate use, so each
chemical is needed to be added /mixed each time, when the disinfectant
solution is required to be used for cleaning the surface.
5. Further, it is also noticed that if dipping duration of the Tasar silkworm eggs
in the chemical solution being used currently i.e. organic acids, peracid etc.,
exceeds even few seconds, then the embryo inside the egg(s) is adversely
affected and which results into poor hatching.
6. The chemical cost for cleaning and sterilization of 100000 Dfls was as high as
Rs. 4000/- and hatching % is comparatively very less (83.15%).
7. The conventional process needs trained persons to perform tasar silkworm
eggs washing and sterilization.
Patent document like CN102125003(A) discloses an electric sterilizing and powder-spraying
device for breeding silkworms. The electric sterilizing and powder-spraying device for
breeding the silkworms comprises a powder discharging pipe, a fan, a power device of the
fan, an operating handle, a powder storage tank, a flow adjusting plate, operating handle, the
powder storage tank is arranged on the upper part of the powder discharging pipe and is
communicated with the powder discharging pipe. The Invention in this document provides
physical apparatus setup for sterilizing eggs shell. However, it is noted that some of the
impurity associated with egg shell does not get off by merely a physical process.
WO2009151912 discloses an antimicrobial composition comprising a pharmaceutically
acceptable surfactant, a monoprotic organic acid comprising a carbon backbone of 4 to 10
carbons.
US 5753493 discloses an egg wash decontaminating solution. The decontaminating solution is
a colloidal emulsion employing saponin capable of reducing the surface tension of a biofilm

allowing for the removal and control of bacteria. This patent utilizes saponin as the active
chemical, which may harm worm's egg during the cleaning and sterilization process.
It is observed that most of the existing processes used for sterilization of egg shell involve
toxic chemicals like peracid, saponin, organic acid etc. which can cause harm to eggs while
performing the wash and sterilization activities. The disinfectant solution disclosed in the
above patent documents are useful for the higher class of vertebrates especially birds like hen
eggs, poultry products, and is active against bacterial pathogens.
Thus, there exists a need in the art for a broad spectrum disinfectant composition which is
user friendly, cost effective and which can be useful for cleaning and sterilization of eggs of
lower class animals, particularly of tasar silkworm which kills/ suppress the Microspridea,
Viral, Bacterial and fungal pathogens.
Also, there is a requirement of a single step and a simple process of cleaning and sterilization
of egg of lower class animals, based on a user friendly and cost effective disinfectant
solution, which is less time taking, cheap, effective in pathogen killing, and which can be
utilized at a commercial scale.
OBJECTIVE(S) OF THE INVENTION:
An object of the present invention is to overcome the drawback / disadvantages of the present
invention.
Another object of the invention is to provide a user friendly, economical and effective
disinfectant for surface sterilization.
Yet another object of the invention is to provide a user friendly, economical and effective
disinfectant for surface sterilization for worm egg surface.
Yet another object of the invention is to provide a disinfectant for surface sterilization of tasar
silkworm, Antheraea mylitta Drury egg.
Yet another object of the invention is to provide a disinfectant with improved hatching
percentage, cocoon production and productivity.
Yet another object of the invention is to provide a disinfectant with shelf life of about 3 years.
Yet another object of the invention is to provide a process for sterilization of a surface.

Yet another object of the present invention is to provide a process for surface sterilization of
tasar silkworm, Antheraea mylitta Drury egg.
Yet another object of the present invention is to provide a single step and a simple process for
surface sterilization.
Summary of the Invention:
Accordingly, in an embodiment of the invention there is a disinfectant solution for surface
sterilization. The disinfectant solution comprises components selected from a group
comprising or any combination(s) thereof:
a) a first antimicrobial agent present in an amount ranging from 5.00 to 8.00%,
b) a second antimicrobial agent present in an amount ranging from 1.50 to
2.00%,
c) a catalyst present in an amount ranging from 1.50 to 2.00%,
d) a solvent present in an amount ranging from 2.00 to 4.00%,
e) an anionic surfactant present in an amount ranging from 5.00 to 8.00%,
f) a nonionic emulsifier present in an amount ranging from 1.00 to 2.00%,
g) an amphoteric detergent present in an amount ranging from 5.00 to 8.00%,
h) a perfume present in an amount ranging from 1.00 to 2.00%), and fresh water
present in an amount ranging from 68 to 83%.
In another aspect of the invention, there is provided a single step process for a surface
washing and sterilization by the above mentioned disinfectant solution. The process
comprises step(s) of treating a surface intended to be sterilized with about 3 to 8%> of said
disinfectant for about 10 minutes with frequent stirring followed by rinsing the treated
surface in running water for about 1 to 2 minutes to obtain the sterilized surface.
BRIEF DESCRIPTION OF THE DRAWINGS:
Figure 1: Schematic illustrates of preparation of Disinfectant solution.

Figure 2: Schematic illustrates of cleaning and surface sterilization of Tasar silkworm eggs
with the disinfectant solution
DETAILED DESCRIPTION OF THE INVENTION:
The Invention in accordance with the object(s), hereby provides a disinfectant solution for
sterilization and cleaning of an egg surface. The egg particularly comprises Tasar silkworm
eggs i.e. Antheraea mylitta drury eggs. The disinfectant solution helps in preventing the risk
of the transmission of all kinds of diseases of Tasar silkworm through the trans ovum.
The disinfectant solution of the present Invention has been named as "Depuratex" henceforth.
The present disinfectant solution is useful for the surface cleaning muchonium strain and
sterilization of tasar silkworm eggs.
In the present embodiment, the invention has been described with reference to the Tasar
silkworm, Antheraea mylitta Drury eggs, however such description should not be considered
as restricting the scope of the present invention. Further, it would be possible for a person
skilled in the art to practice the present Invention considering the disinfectant to be useful for
the sterilization of eggs of any other worm or sterilization of similar kind of object as well
without departing from the scope of the present invention.
The Invention also provides a single step process for sterilizing a surface. The process is user
friendly, simple, single step, easy, economical, and takes only around 10 minutes to complete
egg(s) washing and surface sterilization.
The disinfectant solution comprises active component(s) selected from a group comprising or
any combination(s) thereof:
a) a first antimicrobial agent present in an amount ranging from 5.00 to 8.00%,
b) a second antimicrobial agent present in an amount ranging from 1.50 to
2.00%,

c) a catalyst present in an amount ranging from 1.50 to 2.00%,
d) a solvent present in an amount ranging from 2.00 to 4.00%,
e) an anionic surfactant present in an amount ranging from 5.00 to 8.00%,
f) a nonionic emulsifier present in an amount ranging from 1.00 to 2.00%,

g) ail amphoteric detergent present in an amount ranging from 5.00 to 8.00%,
h) a perfume present in an amount ranging from 1.00 to 2.00%, and fresh water
present in an amount ranging from 68 to 83%.
The said first anti microbial agent comprises Imodium phosphate or any other chemical with
similar chemical properties. This first anti microbial agent also function(s) as a disinfectant, a
degreaser and a cleaning agent.
The second anti microbial agent comprises sodium metasilicate pentahydrate or any other
chemical with similar chemical properties. This second anti microbial agent also function(s)
as a buffering agent, softening agent and a corrosion inhibiting agent
The catalyst component present in the disinfectant solution comprises Urea or any other
chemical with similar chemical properties. This catalyst also works as a whitener and an
additive.
The solvent comprises alcohol like Isopropyl Alcohol. Any other alcohol with similar
chemical properties can also be used in the present disinfectant solution.
Further, the anionic surfactant used in the disinfectant solution is sodium laureth sulphate.
However, it is noteworthy that anionic surfactant(s) is not limited to the use of sodium laureth
sulphate and any other anionic surfactant can also be used in the disinfectant solution.
The amphoteric detergent comprises cocamidopropylbetaine. The amphoteric surfactant(s) is
not limited to the use of cocamidopropylbetaine only and any other amphoteric surfactant can
also be used in the disinfectant.
A non-toxic and non-irritant nonionic emulsifier compound is used in the disinfectant
solution.
The preferred emulsifier is poly ethylene glycol.
The surprising result achieved by this disinfectant solution is that it increases the rate of
hatching of the egg by about 10.32%) as compared to existing disinfectant. The cocoon
harvest is also increased to about 735 cocoons/100dfls. The disinfectant is highly cost
effective and has the extended shelf life of about 3 years.
Advantages of Depuratex:

1. Tasar silkworm eggs washing and sterilization process can be conducted in the same
aqueous solution of Depuratex ( 3 to 8%) and can be conducted in single step.
2. The process is very easy, simple, single step and takes only 10 minutes to complete
egg washing and sterilization.

3. Chemicals which are involved in "Depuratex" are eco-user friendly.
4. The disinfectant solution is safer to use. The employed concentration of the
"Depuratex" i.e. (3 to 8%) does not harm the egg while cleaning the surface. The
solution does not produce any adverse effects if the dipping duration of the tasar
silkworm eggs in Depuratex solution exceeds 30 minutes or so.
5. The disinfectant solution is harmless to the user during egg washing and sterilization.
6. Physical requirement(s) for the process of egg washing and sterilization are very less.
7. The amount of water used in washing egg surface is minimal.
8. Disinfectant is ready made for immediate use. This enables any person to perform the
cleaning process because of simple and easy application process and eliminate the
need of trained person(s).
9. Man power needed for the eggs washing and sterilization process is very less i.e.
100000dfls/30 days and rupees for wages required was very less i.e. 4500/- (Rs. 150 x
30 days). The amount saving in form of wages is Rs. 10,050/- over the existing
technology.
10. Hatching percent is very high which ranged between 92-95% with the average of
93.47%. Gain in hatching % over the existing technology was 10.32%.
11. Better economy

• Less water consumption
• With 1 liter of Depuratex, 20 liters of 5% working solution can be prepared.
• Less manpower needed for the eggs washing and sterilization process which is
15,000dfls/lperson/day.
Cost effectiveness:

1. Depuratex cost is only Rs. 50/- per liter. A litre of Depuratex can be utilized for
5000dfls. So, Depuratex cost for cleaning 100000 Dfls was only Rs. 1000/- and
saving in the chemical cost over the existing technology was Rs. 3500/-.
2. The technology was tested with 3,00,000 dfls in different areas of Jharkhand,
Chhattisgarh, Madhya Pradesh, Maharashtra, Uttar Pradesh and Andhra Pradesh
through the BSMTCs of that particular area. In all cases centers hatching percent was
recorded between 92-95% with the average of 93.47%.
3. In laboratory trials, average cocoons harvested per 100 Dfls were 6683 cocoons. Gain
of cocoons over the existing technology /100 Dfls was 735 cocoons.
Process of preparation of Depuratex:
The Depuratex is prepared by mixing 68 to 83% of clean and fresh water and adding
approximately 5.00 to 8.00% of a first antimicrobial agent like Tri-sodium orthophosphate,
second antimicrobial agent like Sodium metasilicate pentahydrate present in an amount ranging
from 1.50 to 2.00%, catalyst agent like Urea present in an amount ranging from 1.50 to
2.00%, solvent like Isopropyl alcohol present in an amount ranging from 2.00 to 4.00%o, an
anionic surfactant like Sodium laureth sulphate present in the range of 5 to 8%, emulsifier like
polyethylene glycol present in a range 1.00-2.00%), amphoteric detergent like cocamidopropyl
betaine present in the range of about 5.00- 8.00%, perfume like rose perfume present in the
range of about 1.00 to 2.00%, and the required quantity of rose color to make up the final
composition.
The process includes mixing of the mentioned components along with frequent stirring for
about 30 minutes. Figure 1 schematic illustrates of preparation of "Depuratex"
The detailed process for cleaning and sterilization of egg surface by Depuratex is follows:
• 50 ml Tasar Depuratex is added to 1000 ml of clean and fresh water for preparing 5%
concentration solution of Depuratex disinfectant;
• Take Tasar silkworm eggs after the mother moth examination in nylon net;
• Dip the tasar silkworm eggs in the prepared 5% Depuratex disinfectant solution;

• Allow the eggs in the solution for 10 minutes along with frequent stirring;
• Take out the eggs along with the nylon net and rinse/ smooth rub in the running water
for one or two minutes;
• Keeping the surface disinfected Tasar silkworm in thin layer on the news paper/
blotting paper and allowing eggs for drying. Figure 2 shows schematic illustratation
of cleaning and surface sterilization of Tasar silkworm eggs with Depuratex.
Test Studies;
Depuratex was tested in CTR&TI field in the first and second crops of 2011 & 2012. Field
trial was conducted with 3,00,000 Dfls in second crop of 2012 at different fields of
Jharkhand, Chhattisgarh, Madhya Pradesh, Maharashtra, Uttar Pradesh and Andhra Pradesh
through the concerned BSMTCs of that particular area.
The results revealed that, in Depuratex treated batches average hatching % was recorded
93.47% and average cocoons harvested was 6683/100 Dfls. In the case of Sodium Hydroxide
+ Hydrochloric Acid + Formalin (control-existing technology) treated batches average
hatching% was recorded as 83.15% and average cocoons harvested was 5948 per 100 Dfls.
Improvement by using Depuratex over existing Sodium Hydroxide + Hydrochloric Acid +
Formalin tasar silkworm egg washing technology was observed. The gain in hatching % was
10.32%o and cocoons gain per 100 Dfls was 735 cocoons.
Test for substantiating cost effectiveness:
In case of Depuratex chemical cost for treatment of 100000 tasar silkworm Dfls was Rs. 1000
& 30 man days are required. The cost involved for wages to treat 100000 Dfls was Rs. 4500.
Where as in the case of Sodium Hydroxide + Hydrochloric Acid + Formalin treated batches
chemical cost was Rs. 3500, Man days required for treatment was 100 and amount required
for wages was Rs. 15000. By conducting surface cleaning and sterilization of 100000 Dfls.
tasar silkworm eggs with Depuratex, grainures saves a total amount of Rs. 13000/- including
chemical cost of Rs. 2500/- and amount saving in wages Rs. 10500/-.Thus, developed
Depuratex disinfectant for the surface cleaning and sterilization of tasar silkworm eggs is
need based, cost effective, user-friendly and easily adoptable to achieve qualitative and
quantitative progress of tasar culture.
Comparative analysis with existing methods;

The present disinfectant has been compared with already existing methods and the
comparative analysis is following:
Method No. 1:
Soap Treatment:
In this method, tasar silkworm eggs were used to clean with simple soap. The eggs were
taken in nylon net and then soap was applied and rubbed for 15 to 20 minute till the eggs
were cleaned.
Observations:
1. Partial removal of muchonium strains and cleaning was observed.
2. Pathogens were not killed by this method, hence low level of results was observed.
3. In this method, since the eggs are being rubbed with soap physical damage of the eggs
was also observed.
4. Hatching percentage of tasar silkworm eggs was only 73.52%.
5. Chemical cost for cleaning 100000 Dfls was Rs. 3000/-
6. Number of man days required for washing was 80 days and rupees for wages required
was Rs. 12,000/-
7. An average cocoon harvested per 100 Dfls was 5123 cocoons.
Advantages of Depuratex of the present Invention over Soap Treatment:
1. The process is easy, simple, single step and takes only 10 minutes to complete
eggmwashing and sterilization.
2. Excellent cleaning of tasar silkworm eggs obtained by removal of grime and
maintenance of 100% germ free with rapid disinfection with in 10 minutes.
3. Hatching percent is very high which ranged between 92 to 95% with average of
93.47% and gain is over 21% than the soap treatment.

4. Depuratex cost is only Rs. 50/- per liter. A litre of Depuratex can be utilized for
5000dfls. So, Depuratex cost for cleaning 100000 Dfls was only Rs. 1000/- and
amount gain in chemical cost is Rs. 2000/-
5. The manpower needed for the egg washing and sterilization process is very less i.e.
100000dfls/30 days and rupees for wages required was very less i.e. 4500/- (Rs. 150 x
30 days). The amount saving in form of wage is Rs. 10,500/- than the Soap treatment
method.
6. Average cocoons harvested per 100 Dfls were 6683 cocoons. Gain of cocoons over
Soap Treatment A00 Dfls was 1560.
Method No. 2:
Soap cleaning + Formalin:
In this method, tasar silkworm eggs were used to take in nylon net and soap will be applied
and rubbed for 15 to 20 minutes till the eggs were cleaned. There after, tasar silkworm eggs
will be dipped in 3% formalin solution for 10 minutes and allowed for dry in shade.
Observations:
1. Partial removal of muchonium strains and cleaning was observed.
2. Tasar silkworm eggs washing and sterilization process are different and conducted
one'by one in two steps.
3. The process is lengthy, cumbersome process and takes 1 hour for the eggs washing
and sterilization process.
4. Formalin is not eco-user friendly, emits pungent smell and causes cancer disease in
users with which users can not perform optimum level of eggs washing and
sterilization.
5. If the dipping duration of the tasar silkworm eggs in formalin will exceed even few
seconds also then the embryo inside the eggs will die and causes poor hatching.
6. This technology needs trained personals to perform tasar silkworm eggs washing and
sterilization

7. In this method since the eggs are being rubbed with soap physical damage of the eggs
was also observed.
8. Hatching percentage of tasr silkworm eggs was less with 78.62%.
9. Chemical cost for cleaning and sterilization of 100000 Dfls was Rs. 4000/-
10. Number of man days required for washing was 100 days and rupees for wages
required was Rs. 15,000/-
11. An average cocoon harvested per 100 Dfls was only 5514 cocoons.
Advantages of Depuratex over Soap cleaning + Formalin treatment:
1. The process is easy, simple, single step and takes only 10 min to complete eggs
washing and sterilization.
2. Excellent cleaning of tasar silkworm eggs obtained by removal of grime and
maintenance of 100% germ free with rapid disinfection with in 10 minutes.
3. Hatching percent is very high which ranged between 92 to 95% with the average of
93.47% and gain over Soap cleaning + Formalin treatment is 15%.
4. Depuratex cost is only Rs. 50/- per liter. A liter of Depuratex can be utilized for
5000dfls. Depuratex cost for cleaning 100000 Dfls was only Rs. 1000/- and amount
gain in chemical cost is 3000 rupees.
5. The manpower needed for the eggs washing and sterilization process is very less i.e.
100000dfls/30 days and rupees for wages required was very less i.e. 4500/- (Rs. 150 x
30 days). The amount saving in form of wage is Rs. 10,050/- than the Soap cleaning +
Formalin method.
6. Average cocoons harvested per 100 Dfls were 6683 cocoons. Gain of cocoons over
Soap Treatment /100 Dfls was 1169.
Product Evaluation:
Dissolution of Polyhedra of AmCPV and Microsporidea:
In vitro inactivation studies were conducted against disease causing pathogens of tasar
silkworm like Cytoplasmic polyhedrosis virus and Microsporidia with Depuratex and results

are represented in Table 1. The result indicated that Depuratex is very effective in dissolution
of polyhedra of AmCPV and Microsporidea at 5% consentration even at 5 minutes exposer
time also. Where as in case of control (Sodium Hydroxide + Hydrochloric Acid + Formalin)
at 45 seconds dip in Sodium Hydroxide and 30 minutes dip in Hydrochloric Acid + Formalin
solution, partial dissolution of AmCPV and complete dissolution of Microsporidea was
observed.

Growth of Bacteria and Fungi in invitro after sterilization:
Below table 2 represents growth of Bacteria and Fungi in invitro after sterilization with
different disinfectants. The result indicated that Depuratex is very effective in suppression of
Bacteria and Fungi at all tested concentrations at all tested exposure times. Where as in case
of control (Sodium Hydroxide + Hydrochloric Acid + Formalin) at 45 seconds dip in Sodium
Hydroxide and 30 min. dip in Hydrochloric Acid + Formalin solution, partial suppression of
Bacteria and complete suppression of Fungi was observed.



Invivo inactivation studies:
Tasar silkworm eggs surface was contaminated with disease causing pathogens, AmCPV (1 x
104 PIB/ml), Microsopiridia (1 x 104 Spores/ml), Bacteria (10"4 Cells/ml) and P.Citrinum (lx
104 Sp/ml). Cleaning and surface disinfection was conducted as per the process of the Jeevan
Suddi and Sodium Hydroxide + Hydrochloric Acid + Formalin technology (existing
technology) separately and reared up to the cocoon formation. An inoculated control batch
was also maintained and data was recorded.
The results reveled that the Depuratex treated batch hatching % was high with 94.12%
whereas existing technology treated batch hatching % was 83.46%. Hatching % in case of
inoculated control was recorded less (60.13%). The mortality due to Virosis, Bacteriosis and
Fungi was nil in Depuratex treated batch whereas in case of pebrine only 1% of pebrine
incidence was observed (Table 3). In the case of existing technology treated batch mortality
due to Virosis, Microsporidiosis, Bacteriosis and Fungi was 6.33%, 2.00%, 4.80% and 1.15%
respectively. Inoculated control batch mortality due to Virosis, Microsporidiosis, Bacteriosis
and Fungi was 98.83%, 80.60% 58.8% and 53.00, respectively.



Field Test;
Evaluation of Depuratex disinfectant technology was conducted with 3,00,000 Dfls in
different areas of Jharkhand, Chhattisgarh, Madhya Pradesh, Maharashtra, Uttar Pradesh and
Andhra Pradesh through BSMTCs of that particular area (Table 4). In all places hatching
percent was recorded between 92-95% with the average of 93.47%. In the case of control
83.15% average hatching percent was recorded.


Economics for 100000 layings washing and surface sterilization at grainages with different
egg washing technologies was also studied and results are represented in the Table 5. In the
case of all treatments, Depuratex is more economical in all respects. Depuratex treatment
needs 1000 rupees worth of chemicals, 30 man days and 4500 rupees towards wages. In case
of remaining all treatments chemical cost, number of man days and wage amount were very
high.



Improvement of Depuratex over existing technology
Depuratex is more effective as well as more economical than the existing technology. By
adopting newly developed Depuratex tasar silkworm egg washing and surface sterilization
technology both farmers and grainures were benefited (Table 6).
Gain at farmer's level:
The average gain of hatching % was 10.32%, gain in cocoon harvest per 100 dfls was 735
cocoons and extra amount for these gained cocoons was 1103 rupees (@ Rs. 1.50/ cocoon).
Gain at grainage level: (Savings in treatment of 100000 Dfls.)
The Depuratex composition saved a total of 13000 rupees including 2500 rupees chemical
cost and 10500 rupees in saving 70 man days.


The following description is of exemplary embodiments only and is not intended to limit the
scope, applicability or configuration of the invention in any way. Rather, the following
description provides a convenient illustration for implementing exemplary embodiments of
the invention. Various changes to the described embodiments may be made in the function
and arrangement of the elements described without departing from the scope of the invention.
In an embodiment of the present invention, the disinfectant is useful for the sterilization of
surface of an egg shell.
In another embodiment of the present invention, the disinfectant is useful for the sterilization
of surface of an oak tasar silkworm.
In another embodiment of the present invention, the first anti microbial agent comprises
Trisodium phosphate.
In another embodiment of the present invention, the second anti microbial agent
comprisessodium metasilicate pentahydrate.
In another embodiment of the present invention, the solvent comprises Isopropyl Alcohol.
In another embodiment of the present invention, the anionic surfactant comprises sodium
laureth sulphate.
In another embodiment of the present invention, the amphoteric detergent comprises
cocamidopropylbetaine.
In another embodiment of the present invention, the catalytic agent comprises Urea.
In another embodiment of the present invention, emulsifier is poly ethylene glycol.
The invention will now be explained with the help of following examples. However, the
scope of the invention should not be limited to these examples as the person skilled in the art
can easily vary the proportion of the ingredients and combinations.
Example 1:
In an experiment "Depuratex" composition with following components has been prepared:


Results;
At the concentration of the 5% of above mentioned combination chemicals, partial
dissolution of AmCPV was observed at 10 and 15 minutes exposure time (Table 7). No
growth of bacteria and fungi was observed in all tested exposure timings (Table 8). Further,
the surface sterilized eggs showed 85% of hatching (Table 9).




Example 2:
In another experiment "Depuratex" composition with following components has been
prepared:


Results:
At the concentration of the 5% of above mentioned combination chemicals, partial
dissolution of AmCPV and microsporidea was observed at 10 and complete dissolution of
AmCPV and microsporidea was observed 15 minutes exposure time (Table 10). No growth
of bacteria and fungi was observed in all tested exposure timings (Table 11). Further, the
surface sterilized eggs showed 88% of hatching (Table 12).



Example 3:
In another experiment "Depuratex" composition with following components has been
prepared:

Results:
At the concentration of the 5% of above mentioned combination chemicals, partial
dissolution, of AmCPV and Microsporidea was observed at 5 minutes duration where as
complete dissolution was observed at 10 and 15 minutes exposuretime (Table 13). No growth
of bacteria and fungi was observed in all tested exposure timings (Table 14). Further, the
surface sterilized eggs showed 91.5% of hatching (Table 15).



Example 4:
In another set of experiment, Depuratex composition with following components was tested
for efficacy:



At the concentration of 5% of above mentioned combination chemicals, partial dissolution of
AmCPV and microsporidea was observed at 5 minutes exposure time where as complete
dissolution of AmCPV was observed at 10 and 15 minutes exposure time (Table 16). No
growth of bacteria and fungi was observed in all tested exposure timings (Table 17). When
the eggs are surface sterilized, 90% of hatching % was observed (Table 18).



Example 5:
In another experiment, 5% of the Depuratex solution was taken for sterilizing egg surface.
The egg surface was treated with 5% Depuratex solution for about 10 minutes with frequent
stirring. The treated surface is then rinsed with running water for about 2 minutes to obtain
the sterilized surface.
Example 6;
In another set of experiment, Depuratex composition with following components was tested
for efficacy:

At the concentration of the 5% of above mentioned combination chemicals, no
dissolution of AmCPV and microsporidea was observed at 5 and 10 minutes exposure time
where as partial dissolution of AmCPV and microsporidea was observed at 15 minutes
exposure time (Table 19). Partial growth of bacteria and fungi was observed in 5 minutes

exposure but no growth was observed in 10 and 15 minutes exposure (Table 20). When the
eggs are surface sterilized, 80% of hatching % was observed (Table 21).

Example 7:
In another set of experiment, Depuratex composition with following components was tested
for efficacy:


Results:
At the concentration of the 5% of above mentioned combination chemicals, no dissolution of
AmCPV and microsporidia was observed 5 min duration whereas partial dissolution of
AmCPV was observed at 10 minutes and 15 minutes exposure time (Table 22). No growth of
bacteria and fungi was observed in all tested exposure timings (Table 23). When the eggs are
surface sterilized, 86.5% of hatching % was observed (Table 24).

WE CLAIM:
1) A disinfectant solution for surface wash and sterilization, said solution comprises
component(s) selected from a group comprising or any combination(s) thereof:
a) a first antimicrobial agent present in an amount ranging from 5.00 to 8.00%,
b) a second antimicrobial agent present in an amount ranging from 1.50 to
2.00%,
c) a catalyst present in an amount ranging from 1.50 to 2.00%,
d) a solvent present in an amount ranging from 2.00 to 4.00%,
e) an anionic surfactant present in an amount ranging from 5.00 to 8.00%,
f) a nonionic emulsifier present in an amount ranging from 1.00 to 2.00%,
g) an amphoteric detergent present in an amount ranging from 5.00 to 8.00%,
h) an additive(s) present in an amount ranging from 0.002% to 2.00%;
i) fresh water present in an amount ranging from 68 to 83%).
2. The disinfectant solution as claimed in claim 1, wherein said surface comprises but
not limited to surface of an egg.
3. The disinfectant solution as claimed in claim 2, wherein said egg is ofAntheraea
mylitta.
4. The disinfectant solution as claimed in claim 1, wherein said first antimicrobial agent
comprises Trisodium phosphate.
5. The disinfectant solution as claimed in claim 1, wherein said second antimicrobial
agent comprises sodium metasilicate pentahydrate.
6. The disinfectant solution as claimed in claim 1, wherein said solvent comprises
Isopropyl alcohol.

7. The disinfectant solution as claimed in claim 1, wherein said anionic surfactant
comprises sodium laureth sulphate.
8. The disinfectant solution as claimed in claim 1, wherein said amphoteric detergent
comprises cocamidopropylbetaine.
9. The disinfectant solution as claimed in claim 1, wherein said nonionic emulsifier is
poly ethylene glycol.
10. The disinfectant solution as claimed in claim 1, wherein said additive(s) is selected
from a group comprising of perfume present in an amount ranging from 1.00 to
2.00%, rose color present in an amount of about 0.002%, or combination(s) thereof;
11. The disinfectant solution as claimed in claim 1, wherein said disinfectant solution
increases the rate of hatching of said egg by about 10.32% as compared to existing
disinfectant(s).
12. The disinfectant solution as claimed in claim 1, wherein said disinfectant increases the
coccon harvestment to about 735 coccoons/100dfls (7.35%/ldfl).
13. The disinfectant solution as claimed in claim 1, wherein said disinfectant has shelf life
of about 3 years.
14. The disinfectant solution as claimed in claim 1, wherein said disinfectant is
economical by 300%) as compared to the existing disinfectant.
15. A single step process for a surface wash and sterilization by a disinfectant solution,
said process comprising step(s) of:
a) treating said surface with about 3 to 8% of said disinfectant solution for about 10
minutes with frequent stirring:
b) rinsing said treated surface resulting from step (a) in running water for about 1 to 2
minutes to obtain the sterilized surface.
16. The process as claimed in claim 14, wherein said disinfectant solution is of claim(s) 1
to 1.3.
17. The process as claimed in claim 14, wherein said surface comprises but not limited to
surface of an egg.

18. The process as claimed in claim 16, wherein said egg is of Antheraea mylitta.

ABSTRACT

The invention relates to a disinfectant solution for cleaning and surface sterilization of Tasar
silkworm, Antheraea mylitta Drury eggs. The disinfectant solution comprises anti microbial
agent(s), catalyst, solvent, surfactant, emulsifier, detergent etc. The solution also increases the
rate of hatching of eggs and coccon harvestment.
The Invention further extends to provide a process for surface sterilization of oak tasar
silkworm worm eggs by utilizing the disinfectant solution.